HTA HT2800T User manual

HT2800T_en_c.doc
User Guide
HT2800T
All-in-ones GC Autosampler:
Headspace, Liquid and SPME
www.hta-it.com

HT2800T_en_c.doc 06/03/2017
This manual contains information about the product(s) indicated on the cover of this document.
The information contained in this document is subject to change without notice.
This document is for information only, the manufacturer accepts no liability for errors
contained herein or for incidental or consequential damage with the furnishing, performance, or
use of this material.
Unless otherwise specified, each reference to names or belongings is purely casual and has
the sole purpose of illustrating the product described here. Contents of this publication may
not be reproduced in any form or by any means (including electronic storage and retrieval or
translation into a foreign language), without prior agreement and written consent from the
copyright owner.
All rights are reserved.
Manufacturer: HTA s.r.l. - 77-79, via del Mella, I-25131 Brescia (BS), Italy - Tel: [phone] -
E-mail:[e-mail]

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Summary
1 INTRODUCTION........................................................................................................................9
1.1 I NTENDED U SE AND R ESTRICTIONS .........................................................................................9
1.2 S YMBOL T ABLE ...................................................................................................................10
1.3 W ARNING ...........................................................................................................................11
1.4 S TANDARDS C OMPLIANCE ...................................................................................................14
1.5 G ENERAL S PECIFICATION ....................................................................................................14
1.6 F UNCTIONS AVAILABLE ONLY THROUGH HTA A UTOSAMPLER M ANAGER ....................................15
1.6.1 Vial Leakage Check (Headspace Mode) .........................................................................................15
1.6.2 Method development option (Headspace Mode).............................................................................15
1.7 V ERSIONS ..........................................................................................................................15
1.7.1 Description......................................................................................................................................15
1.7.2 Options............................................................................................................................................16
1.8 T ECHNICAL S PECIFICATIONS ................................................................................................17
1.9 PC S OFTWARE R EQUIREMENTS (HTA A UTOSAMPLER M ANAGER )............................................20
2 EQUIPMENT DESCRIPTION...................................................................................................21
2.1 P ARTS D EFINITION ..............................................................................................................21
2.1.1 HT2800T/HT2850T Overview.........................................................................................................21
2.1.2 Syringe location ..............................................................................................................................27
2.1.3 Connection panel ............................................................................................................................35
2.2 M OVING PARTS ...................................................................................................................36
2.3 T OOL KIT ............................................................................................................................37
2.4 T OUCH SCREEN DISPLAY DESCRIPTION ..................................................................................38
2.4.1 Touchscreen use..............................................................................................................................38
2.4.2 Limited Warranty............................................................................................................................42
3 INSTALLATION .......................................................................................................................44
3.1 U NPACKING AND P RELIMINARY O PERATIONS .........................................................................44
3.2 A UTOSAMPLER A SSEMBLY ...................................................................................................45
3.2.1 How to lift and carry the autosampler............................................................................................49
3.3 I NSTALLATION .....................................................................................................................49
3.3.1 Autosampler installation on the mounting plate .............................................................................51
3.3.2 Safety Lock setting ..........................................................................................................................56
3.3.3 Electrical connections.....................................................................................................................57
3.3.4 Purge line connections (for Headspace and Fiber Cleaning Device).............................................58
3.3.5 Preparing the solvent and waste vials (liquid mode)/ Preparing the washing vial (SPME mode) .59

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3.4 S TART -U P ..........................................................................................................................60
3.5 PC CONNECTION AND SOFTWARE INSTALLATION .....................................................................61
3.5.1 Autosampler controlled by LAN-connected PC ..............................................................................61
3.5.2 Autosampler connected directly to the PC......................................................................................70
3.6 A CCESSORIES INSTALLATION ................................................................................................71
3.6.1 External pressure regulator installation (only for Headspace and SPME mode)....................71
3.6.2 Swagelok Adapter installation (only forHeadspace and SPME mode).......................................72
3.6.3 System integrity tool installation (only for Headspace mode).........................................................73
3.6.4 Vial Locator: Installing and removing (Liquid mode) ....................................................................75
3.7 P REPARING SAMPLE VIAL .....................................................................................................78
3.7.1 Vial filling in liquid mode ...............................................................................................................79
3.7.2 Vial capping (Screw Cap)...............................................................................................................79
3.7.3 Vial capping (Crimping Cap)..........................................................................................................79
3.8 F INAL INSTALLATION OPERATIONS .........................................................................................83
3.9 B ASIC TRAINING ..................................................................................................................83
4 SET UP OPERATIONS............................................................................................................84
4.1 V IEW P ARAMETERS .............................................................................................................84
4.2 S ETUP ...............................................................................................................................85
4.2.1 Setup: Alignment.............................................................................................................................85
4.2.2 Setup: Syringe warmer assembly installation/ replacement (Headspace mode)............................92
4.2.3 Syringe installation/ replacement (liquid mode)...........................................................................101
4.2.4 SPME Fiber holder installation/ replacement ..............................................................................111
4.2.5 Setup: Manual operations.............................................................................................................119
4.2.6 Setup: Configuration.....................................................................................................................123
4.2.7 Setup: Service................................................................................................................................135
5 PROGRAMMING METHOD...................................................................................................136
5.1 M ETHOD (H EADSPACE MODE ) ............................................................................................136
5.1.1 Method Menu................................................................................................................................136
5.1.2 Method X: Setting method parameters..........................................................................................136
5.1.3 Method tools..................................................................................................................................140
5.1.4 Example ........................................................................................................................................140
5.2 M ETHOD (L IQUID MODE ) ....................................................................................................141
5.2.1 Method Menu................................................................................................................................141
5.2.2 Method X: Setting method parameters..........................................................................................142
5.2.3 Method tools..................................................................................................................................146
5.2.4 Example ........................................................................................................................................146
5.3 M ETHOD : SPME MODE .....................................................................................................147

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5.3.1 Method Menu................................................................................................................................147
5.3.2 Method X: Setting method parameters..........................................................................................147
5.3.3 Method tools..................................................................................................................................153
5.3.4 Example ........................................................................................................................................153
6 PROGRAMMING SEQUENCE...............................................................................................155
6.1 S EQUENCE : H EADSPACE MODE ..........................................................................................155
6.1.1 Sequence Menu .............................................................................................................................155
6.1.2 Setting sequence step parameters .................................................................................................155
6.1.3 Sequence tools...............................................................................................................................156
6.2 S EQUENCE : L IQUID MODE ..................................................................................................157
6.2.1 Sequence Menu .............................................................................................................................157
6.2.2 Setting sequence step parameters .................................................................................................157
6.2.3 Sequence tools...............................................................................................................................159
6.3 S EQUENCE : SPME MODE ..................................................................................................159
6.3.1 Sequence Menu .............................................................................................................................159
6.3.2 Setting sequence step parameters .................................................................................................160
6.3.3 Sequence tools...............................................................................................................................161
7 OPERATIONS.......................................................................................................................162
7.1 H OME SCREEN ..................................................................................................................162
7.1.1 Home screen: Standard User Interface.........................................................................................162
7.1.2 Home screen: Quick Start User Interface.....................................................................................164
7.2 S INGLE INJECTION .............................................................................................................165
7.3 A UTOMATIC RUN ...............................................................................................................166
7.3.1 Interrupting an automatic run.......................................................................................................167
7.3.2 Immediate Injection.......................................................................................................................167
7.3.3 Skip Next Vial option.....................................................................................................................167
7.3.4 Priority injection...........................................................................................................................168
7.4 S YRINGE PURGE ...............................................................................................................169
7.5 S YRINGE WASH .................................................................................................................169
7.6 F IBER C LEANING /C ONDITIONING .........................................................................................170
7.7 L OAD AND U NLOAD S AMPLE V IAL .......................................................................................170
7.8 L OAD AND U NLOAD S OLVENT V IALS ....................................................................................171
7.9 S ETTINGS M ENU ...............................................................................................................171
7.9.1 Change tray/rack type...................................................................................................................171
7.9.2 Eco Savings...................................................................................................................................173
7.10 C HANGE M ODE .................................................................................................................174

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7.10.1 Change from Headspace to Liquid mode..................................................................................175
7.10.2 Change from SPME to Liquid mode .........................................................................................182
7.10.3 Change from Liquid to Headspace mode..................................................................................189
7.10.4 Change from Liquid to SPME mode .........................................................................................195
7.10.5 Change from Headspace to SPME mode..................................................................................201
7.10.6 Change from SPME to Headspace mode..................................................................................204
8 TROUBLESHOOTING...........................................................................................................207
8.1 I NTRODUCTION .................................................................................................................207
8.2 S TART UP ERRORS ............................................................................................................208
8.2.1 Safety lock error............................................................................................................................208
8.2.2 Vial locator error..........................................................................................................................208
8.2.3 Memory error................................................................................................................................209
8.3 M ETHOD ERRORS .............................................................................................................209
8.3.1 Fill Volume/Pull Up Strokes setting incompatibility.....................................................................209
8.3.2 Fill volume/Syringe Pre-Fill setting incompatibility.....................................................................210
8.3.3 Internal Standard setting error.....................................................................................................210
8.3.4 “Sample washes” setting error.....................................................................................................211
8.4 T OUCH S ENSOR PROBLEMS ...............................................................................................211
8.5 F ALLING VIALS ..................................................................................................................211
8.6 O BSTACLE FOUND .............................................................................................................212
8.7 O BSTACLE FOUND DURING COVER MOVEMENT .....................................................................213
8.8 L ACK OF A V IAL ( WASTE OR SOLVENT VIAL FOR LIQUID MODE , WASHING VIAL FOR SPME MODE ) 213
8.9 M ISMATCH ERRORS ...........................................................................................................214
8.9.1 Syringe wash mismatch error........................................................................................................214
8.9.2 Single injection mismatch error....................................................................................................214
8.9.3 Automatic run mismatch errors ....................................................................................................215
8.10 M ISSING ERRORS ..............................................................................................................217
8.10.1 Missing: rear injector alignment ..............................................................................................217
8.11 T IMEOUT ERROR ...............................................................................................................218
8.11.1 Peripheralcommunicationtimeout............................................................................................218
8.12 S YRINGE ID TIMEOUT .........................................................................................................218
8.13 M ECHANICAL ERRORS .......................................................................................................219
8.14 H EATING ERRORS .............................................................................................................219
8.15 S YSTEM INTEGRITY TEST FAIL .............................................................................................220
8.16 D AMAGE TO SYRINGE NEEDLE ............................................................................................220
8.16.1 Syringe inspection.....................................................................................................................221
8.17 T RAY EMERGENCY RELEASE ...............................................................................................222

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8.17.1 Tray opening by hand ...............................................................................................................222
8.18 “HTA A UTOSAMPLER M ANAGER ” TROUBLESHOOTING ...........................................................224
8.18.1 Autosampler and PC do not communicate (connection by LAN) ......................................224
8.19 A NALYTICAL TROUBLESHOOTING .........................................................................................226
8.19.1 Analytical troubleshooting (Headspace Mode).........................................................................226
8.19.2 Analytical troubleshooting (Liquid Mode)................................................................................228
8.20 B AR C ODE R EADER T ROUBLESHOOTING .............................................................................230
9 MAINTENANCE.....................................................................................................................232
9.1 O RDINARY MAINTENANCE ..................................................................................................232
9.1.1 Cleaning of vial rack/tray .............................................................................................................232
9.1.2 External cleaning of cabinet .........................................................................................................233
9.1.3 Incubation oven cleaning..............................................................................................................233
9.1.4 Touch screen display cleaning......................................................................................................234
9.1.5 Syringe replacement in the syringe warmer assembly (Headspace mode)...................................234
9.1.6 Fiber replacement in the Fiber holder (SPME mode)...................................................................241
9.1.7 Septum of the System integrity tool replacement (Headspace mode)............................................243
9.1.8 Preventive maintenance pack........................................................................................................243
9.2 E XTRAORDINARY MAINTENANCE .........................................................................................244
10 UNINSTALLING AND DISPOSAL ......................................................................................245
10.1 A UTOSAMPLER UNINSTALLING ............................................................................................245
10.2 A UTOSAMPLER SHIPPING ...................................................................................................246
10.3 A UTOSAMPLER DISPOSAL ...................................................................................................250
10.4 D ISPOSAL INSTRUCTIONS ...................................................................................................251
11 APPENDIX A – GLOSSARY ..............................................................................................252
11.1 H EADSPACEMODE ............................................................................................................252
11.2 L IQUIDMODE ....................................................................................................................253
11.1 SPME MODE ....................................................................................................................254
12 APPENDIX B – CONSUMABLES.......................................................................................256
12.1 C ONSUMABLES FOR H EADSPACE MODE ...............................................................................256
12.1.1 Syringes.....................................................................................................................................256
12.1.2 Sample vials..............................................................................................................................256
12.2 C ONSUMABLES FOR L IQUID MODE .......................................................................................260
12.2.1 Syringes.....................................................................................................................................260
12.2.2 Sample vials..............................................................................................................................263
12.2.3 Solvent and waste vials.............................................................................................................266

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12.3 C ONSUMABLES FOR SPME MODE .......................................................................................266
12.3.1 Fibers........................................................................................................................................266
12.3.2 Washing vial .............................................................................................................................266
13 APPENDIX C – ANALYZER CONNECTOR........................................................................267
13.1 RS232C INTERFACE ( OPTIONAL ) ........................................................................................269

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1 Introduction
1.1 Intended Use and Restrictions
HT2800T is an all-in-ones GC autosampler for static Headspace analysis, liquid sample injection
and SPME.
The autosampler must be used in a professional environment, and only by properly trained
users. The autosampler must be used according to the regulations in force regarding the safety
in testing laboratories.
A list (not exhaustive) of typical applications of the autosampler is reported below:
• Environmental analysis
• Quality control in the chemical and pharmaceutical industry
• Studies related to pharmaceuticals
• Drug discovery
Other techniques or areas of application are possible.
The safety measures and precautions regarding use, maintenance and repair, are not intended
for applications that require the use of toxic or infectious substances.
Every possible mixture or omission of a component cannot be recognized or previously
prevented. The operator that will use the autosampler must be trained about the used
substances, their hazards, their use and their proper disposal. For the correct use of the used
substances, refer to chemicals safety data sheets (MSDS) and to the regulations, laws,
directives and safety decrees currently in force that regulate the behaviour inside the testing
laboratories.
The insertion and positioning of the sample (vial), the insertion of syringes and all the other
preparation activities are under the sole responsibility of the user. The user must operate in
accordance with the law, in particular the ones concerning the safety of testing laboratories and
accident prevention.

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The operator must be trained to operate the equipment in accordance with the safety standards
for the laboratories. The autosampler does not have specific protection mechanisms against
substances that are corrosive, potentially infectious, toxic and radioactive or against other
substances that can be hazardous to health. However, when the autosampler is used, because
of the handled substances, the user could need to use individual protection measures such as
gloves, masks and goggles; the use of such devices must comply with the regulations currently
in force regarding the safety of testing laboratories.
The use of the autosampler for any technique invasive for humans or animals is explicitly
prohibited. The autosampler must be used only in the manner described in the usage
instructions. Any other use may affect the autosampler and operator safety.
HTA s.r.l. is not responsible for damage caused, even only in part, by improper use of the
autosampler, by unauthorized modification of it, by different or missing execution of the
procedures described in this manual, or by the use of the autosampler violating the applicable
laws and regulations.
The autosampler has been designed and tested in accordance with current safety standards.
1.2 Symbol Table
Please carefully read this table before using your autosampler. These symbols are used in this
manual.
Do not touch: action forbidden
Warning: check the manual
High voltage
Warning: indicates pinch, crush and cut hazard
Warning: indicates the possible presence of biological or hazardous substances
Hot surface

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1.3 Warning
Read the following warnings before unpacking or using the unit.
See paragraph 1.1 “Intended Use and Restrictions “ and be sure you understand it.
Read Declaration of CE Compliance enclosed with the autosampler.
Before removing the autosampler from the box, please carefully read paragraphs 3.1 “Unpacking
and Preliminary Operations” and 3.2 “Autosampler Assembly”.
Before switching theautosampler ON check the correct voltage.
Electrical Hazards. Every analytical instrument has specific hazards, so ensure you read and
comply with the following precautions.
Use only the power supply provided with the autosampler. The use of a different system could
cause damage to the equipment and/or compromise safety. There are no operator-serviceable or
replaceable parts inside the power supply. In case of failure, contact the Technical Assistance
Centre. Violations of these directives imply the immediate invalidation of the warranty and service
contract.
The supplied power cord must be inserted into a power outlet with a protective earth contact
(ground). When using an extension cord, make sure that the cord also has an earth contact.
Do not change the external or internal grounding connections. Tampering with or disconnecting
theseconnections could endanger you and/or damage the autosampler.
The product is designed and tested in accordance with international safety standards.
You do not need to make any changes to the electrical connections or to the chassis of the
autosampler in order to ensure safe operation.
Do not turn the autosampler on if you suspect that it has incurred any kind of electrical damage or
if it shows visible signs of damage. If, for any reason, the safety protections were
compromised or the autosampler shows visible signs of damage, disconnect the power cord and
contact a HTA representative. The power cord connector is considered the disconnecting device
of the product. Electrical damage may have occurred if the autosampler shows visible signs of
damage, or has been transported under severe stress.

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The operator is responsible for adequate decontamination in case of dangerous spills over or
inside the autosampler. The operator is also responsible for the proper use of cleaning or
decontamination agents. The agents shall not cause hazards due to a reaction with the
autosampler or other materials contained inside. If there is any doubt about the method for
proper use of cleaning or decontamination agents contact a HTArepresentative.
With regards to autosampler recycling and disposal the following operations mus be performed in
the proper order: 1) remove syringe, samples and reagents; 2) switch off the autosampler; 3)
remove any contamination of the instrument in accordance with national and international safety
regulations.
Damage can also result if the autosampler is stored for prolonged periods under unfavourable
conditions (e.g. subjected to heat, water, etc.).
Do not place the autosampler in a position which makes it difficult to handle the disconnecting
device of the autosampler.
Always disconnect the power cord before attempting any type of maintenance.
Capacitors inside the autosampler may still be charged even if the autosampler is turned off.
The autosampler includes a number of integrated circuits. These circuits may be damaged if
exposed to excessive line voltage fluctuations and/or power surges.
If, for technical reasons, it is necessary to work on autosampler parts which are only accessible
using a tool, contact the Technical Assistance Centre. Violation of this automatically invalidates
the warranty and service contract. These restrictions do not affect normal operations such as
syringe installation and removal.
Do not make contact with the autosampler while it is running.
Do not try to open or close the sample tray manually, unless it is strictly necessary. In this case,
follow the instructions of paragraph 8.17.1 “Tray opening by hand”
To avoid accidents, observe the rules of good laboratory practice while handling solvents,
reagents and consumables. Observe the safety regulations regarding the use of chemicals being
used, as indicated in their specific Material Safety Data Sheets (MSDS). Carefully check the
physical and chemical properties of all substances before use.
Do not use vials without sealing caps. Vapors from volatile solvents may be hazardous and
flammable. Acidic vapors may be corrosive when they come in contact with mechanical parts.

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If, during the start up process or while the running the machine, you notice unusual noises or
vibrations, stop the operating process and contact the TechnicalAssistance Centre.
The warning signals presented on the turret and on the oven cover indicate the presence of
moving parts, including the syringe and the fan. Be careful to avoid bumps or cuts.
The biohazard warnings presented on the turret and on the oven cover indicate the possible
presence of biological or hazardous substances, depending on the types of substances used with
theautosampler.
The autosampler can heat the vials in the oven and the sample in the syringe up to 170°C. For
this reason, the oven and the syringe holder are labelled as hot surfaces. Be careful to avoid
burns. Allow the heated syringe adapter to cool down before attempting to change or inspect the
syringe. Do not touch the vials, without adequately protected hands, for at least 30 minutes after
the vial is removed from the oven.
The autosampler can heat the vials in the oven and the sample in the syringe up to 170°C. The
autosampler is not intended to be used with a large quantity of flammable substances. Verify,
according to an analysis of the laboratory's safety standards and safety cards of chemical
substances, that substances can be used at this temperature without any risk to the operator
and/or the environment.
The oven is equipped with a fan, which is used to assist temperature stabilization and the cooling
down of the oven.
Thisfan must not be obstructed.Any obstruction may affect the operation of the unit.
The autosampler must be located in a well ventilated environment,
Environmental temperature must be from 15°C to 35°C and relative humidity must be between 5-
80% (no condensation). If these environmental conditions are not met, it may affect the
performance or damage the unit.
Do not obstruct the air outlet opening of the instrument. Keep an appropriate distance between
the rear and the side of the machine and any other items.

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During Headspace/SPME operation the syringe is cleaned after each injection with inert, clean
gas (e.g. N2 or He). Therefore a gas supply line must be available close to the autosampler. Do
not use flammable purging gases. Do not use hydrogen as a purging gas. Hydrogen creates a
potentialexplosion hazard due to the ventingof gases.
Only if the Bar code Reader option has been purchase and it is enabled: do not view directly the
led light with optical instruments such as magnifiers, eye loupes or microscopes within a distance
of 100mm. This could cause serious eye injury. The Bar code Reader installed is a Class 1 LED
Product with Class 1 internal radiation. Maximum LED Output: 141,2 uW.
Be careful while handling the syringe holder of the SyringeID system (see 1.7.2 Options” and 11.2
“Liquid mode”). Do not touch the connector or the electronic circuit placed on the back of the
holder. The components may be damaged. If not used, the SyringeID holder must be stored in the
antistatic bag supplied with the packaging.
1.4 Standards Compliance
The applicable rules for this autosampler are the following:
• 2014/35/EU Low-Voltage Equipment Directive.
• 2014/30/EU Electromagnetic Compatibility Directive.
• 2011/65/EU Restriction of the use of certain hazardous substances in electrical and
electronic equipment (RoHS) Directive.
• 2006/42/EC Machinery Directive.
1.5 General Specification
This is an autosampler for gas chromatography.
The HT2800T combines the functions of an autosampler for liquid, static Headspace and SPME
into a single unit.
The autosampler is compatible with most Gas Chromatographs (GC).
It can be installed on GCs with either one or two injectors.In the case of GCs with two injectors,
your autosampler can be configured to inject automatically into one or the other injector.
The autosampler can be programmed by using the touch screen display or by a PC using an
Ethernet or RS232C connection (optional).

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1.6 Functionsavailable only through HTA Autosampler
Manager
1.6.1 Vial Leakage Check (Headspace Mode)
HTA Autosampler Manager allows the user to enable the Vial Leakage Check (ACCESS
SEQUENCE: Setup-Options).
If this option is enabled, the pressure inside vials is monitored by an heuristic procedure in order
to check against anomalous values that are indicative of a vial leakage problem.
The Vial leakage test can discriminate for samples which are correctly sealed vs. bad crimping
or missing septa. Each sample is marked with PASS or FAIL information in HTA Autosampler
Manager Windows.
1.6.2 Method development option (Headspace Mode)
During method development it can be useful to test methods characterized by a progressive
increment of a specific parameter in order to define which are the best settings for this
parameter.
The parameters that can be increased in this way are the syringe/oven temperature and the
incubation time.
This option is only available with “HTA Autosampler Manager”, using the “Method development”
tab. For the detailed explanation of this option, please refer to “HTA Autosampler Manager”
online help.
1.7 Versions
1.7.1 Description
The autosampler is available in the following versions:
HT2800T
This version is equipped with a touch screen interface.
It includes Headspace and Liquid kit (SPME can be sourced separately, also at a later time).
HT2850T

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This version is equipped with a touch screen interface.
It includes Headspace and Liquid kit (SPME can be sourced separately, also at a later time). It
also includes SPME cleaning device.
1.7.2 Options
Available for all versions:
1.91.716 – SPME Kit for HT28XXT
1.90.501 - HTAAutosampler Manager (Standard Version)
PC software. This may be sold as an option. Please check with your service
representative.
1.90.502 - HTAAutosampler Manager (CFR 21 Part 11)
PC software compliant with CFR 21 Part 11 regulation. This may be sold as an option. Please
check with your service representative.
1.91.740 – GLP Pack for HT2800T/HT2850T
It includes:
System integrity kit (for Headspace Mode)
The System integrity test is used to verify the syringe integrity. This test can easily be
executed at the beginning of each batch in a very convenient way. This functionality is made in
a heuristic approach (for detailed description of this function see paragraph 11 “Appendix A –
Glossary”). To see how to install this tool see paragraph 3.6.3 “System integrity tool installation
(only for Headspace mode)”.
SyringeID system (for Liquid Mode)
SyringeID is an automatic syringe recognition system. This system is based on RFID tags and
it is able to identify syringes in an unambiguos way. For detailed description of this function see
paragraph 11 “Appendix A – Glossary”.
1.91.734 - Bar Code Reader Module HT2800T (Factory installation)
This may be sold as an option. This is not compatible with RS232C interface option.
The following accessories can be supplied upon request:
HTA Part Number Description
3.23.000 External Pressure Regulator. For installation see 3.6.1
1.93.822 Swagelok adapter for HT2x00x series. For installation see 3.6.2
1.93.820 Spacers kit for 10ml vial (6pc/pk)
1.93.828 Spacers kit for 6ml vial (6pc/pk)

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1.91.280 Syringe kit 1ml for Headspace autosampler
C00028 Manual Crimper for 20mm Caps
C00029 Manual De-Crimper for 20mm Caps
1.8 Technical Specifications
DIMENSIONS
HT2800T/HT2850T
Height 640mm
Width (closed oven cover) 330mm
Width (open oven cover) 350mm
Depth (closed tray) 320mm
Depth (open tray) 520mm
WEIGHT
HT2800T 10kg
HT2850T 10kg
ELECTRICAL SPECIFICATIONS
Power supply:
Voltage 100-240 ±10%Vac
Frequency 47-63Hz
Max Current 1.6 A
Autosampler HT2800T/HT2850T:
Voltage 24Vdc
Power 120W
INTERFACE SPECIFICATIONS
To GC TTL
To PC Ethernet 10/100
Optional: RS232C (available on a limited number of models)
Ethernet cable should be Category 5 (or higher)
RS232 cable: max distance PC – autosampler: 1.80m (opt. 3.50m)

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ENVIRONMENT CONDITIONS
Room temperature limits 15°C - 35°C
Ambient humidity limits 5% - 80% (not-condensable)
Maximum altitude 2000m
SOUND PRESSURE LEVEL
Maximum measured level 60dBA (below the limits of 85dBA defined by the
regulations in force)
SAFETY INFORMATION
The autosampler is classified as shown below: Pollution degree 2
Overvoltage category II
Devices for use indoors
ANALYTICAL PARAMETERS
HEADSPACE MODE
Tray capacity 42 vials (20ml); optional: 6 and 10ml
Syringe volume 2.5ml (standard); optional:1ml
Maximum pressure in the injector 2.5bar
(2.5ml syringe)
Conditioning
Oven positions 6
Oven Temperature off, from 40°C to 170°C
Incubation time from 0 to 999min step 1min
Shaking method orbital
Shaker speed from very low to very high
Shaking cycles on/off, from 0 to 9.9min
Sampling
Syringe Temperature off, from 40°C to 150°C
Sample volume 0.01ml stepwise
Pull Up Strokes up to 15 strokes
Sample speed from 0.5 to 100ml/min (40ml/min for 1ml syringe)
Injection

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Injection speed from 0.5 to 100ml/min (40ml/min for 1ml syringe)
Pre/Post injection dwell time from 0 to 99secs
Enrichment cycles up to 15
Dwell time between enrichment cycle from 0 to 100min
Syringe purge
Flush time from 0 to 99min
Purge temperature off, from 40°C to 150°C
LIQUID MODE
General
Tray capacity 121 vials (2ml)
Volume Increment/Decrement According to syringe type
Pre&Post wash
Number of washes up to 15
Wash mode each injection, each sample or step
Wash with sample
Sample wash up to 15
Bubble elimination up to 15 strokes
Sampling withdrawal
Sample Volume According to syringe type
Air Volume According to syringe type
Aspiration speed According to syringe type
Viscosity time from 0 to 15secs
Injection
Injection speed According to syringe type
Waiting time before and after injection from 0 to 99secs
Internal Standard Techniques
Mode no, post or double
SPME MODE

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Tray capacity 42 vials (20ml); optional: 6 and 10ml
Extraction Mode liquid phase/Headspace vapors
Fiber type 10mm, 20mm
Fiber Cleaning Station only for HT2850T (see dedicated appendix)
Conditioning
Oven positions 6
Oven Temperature off, from 40°C to 170°C
Incubation time from 0 to 999min step 1min
Shaking method orbital
Shaker speed from very low to very high
Shaking cycles on/off, from 0 to 9.9min
1.9 PC Software Requirements (HTA Autosampler Manager)
See HTA Autosampler Manager Online Help for the related PC specifications.
Warning
The virtual screen is available only with LAN connection. It is not available with RS232
connection.