HTA HT2800T User manual

HT2800T_en_c.doc
User Guide
HT2800T
All-in-ones GC Autosampler:
Headspace, Liquid and SPME
www.hta-it.com

HT2800T_en_c.doc 06/03/2017
This manual contains information about the product(s) indicated on the cover of this document.
The information contained in this document is subject to change without notice.
This document is for information only, the manufacturer accepts no liability for errors
contained herein or for incidental or consequential damage with the furnishing, performance, or
use of this material.
Unless otherwise specified, each reference to names or belongings is purely casual and has
the sole purpose of illustrating the product described here. Contents of this publication may
not be reproduced in any form or by any means (including electronic storage and retrieval or
translation into a foreign language), without prior agreement and written consent from the
copyright owner.
All rights are reserved.
Manufacturer: HTA s.r.l. - 77-79, via del Mella, I-25131 Brescia (BS), Italy - Tel: [phone] -
E-mail:[e-mail]

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Summary
1 INTRODUCTION........................................................................................................................9
1.1 I NTENDED U SE AND R ESTRICTIONS .........................................................................................9
1.2 S YMBOL T ABLE ...................................................................................................................10
1.3 W ARNING ...........................................................................................................................11
1.4 S TANDARDS C OMPLIANCE ...................................................................................................14
1.5 G ENERAL S PECIFICATION ....................................................................................................14
1.6 F UNCTIONS AVAILABLE ONLY THROUGH HTA A UTOSAMPLER M ANAGER ....................................15
1.6.1 Vial Leakage Check (Headspace Mode) .........................................................................................15
1.6.2 Method development option (Headspace Mode).............................................................................15
1.7 V ERSIONS ..........................................................................................................................15
1.7.1 Description......................................................................................................................................15
1.7.2 Options............................................................................................................................................16
1.8 T ECHNICAL S PECIFICATIONS ................................................................................................17
1.9 PC S OFTWARE R EQUIREMENTS (HTA A UTOSAMPLER M ANAGER )............................................20
2 EQUIPMENT DESCRIPTION...................................................................................................21
2.1 P ARTS D EFINITION ..............................................................................................................21
2.1.1 HT2800T/HT2850T Overview.........................................................................................................21
2.1.2 Syringe location ..............................................................................................................................27
2.1.3 Connection panel ............................................................................................................................35
2.2 M OVING PARTS ...................................................................................................................36
2.3 T OOL KIT ............................................................................................................................37
2.4 T OUCH SCREEN DISPLAY DESCRIPTION ..................................................................................38
2.4.1 Touchscreen use..............................................................................................................................38
2.4.2 Limited Warranty............................................................................................................................42
3 INSTALLATION .......................................................................................................................44
3.1 U NPACKING AND P RELIMINARY O PERATIONS .........................................................................44
3.2 A UTOSAMPLER A SSEMBLY ...................................................................................................45
3.2.1 How to lift and carry the autosampler............................................................................................49
3.3 I NSTALLATION .....................................................................................................................49
3.3.1 Autosampler installation on the mounting plate .............................................................................51
3.3.2 Safety Lock setting ..........................................................................................................................56
3.3.3 Electrical connections.....................................................................................................................57
3.3.4 Purge line connections (for Headspace and Fiber Cleaning Device).............................................58
3.3.5 Preparing the solvent and waste vials (liquid mode)/ Preparing the washing vial (SPME mode) .59

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3.4 S TART -U P ..........................................................................................................................60
3.5 PC CONNECTION AND SOFTWARE INSTALLATION .....................................................................61
3.5.1 Autosampler controlled by LAN-connected PC ..............................................................................61
3.5.2 Autosampler connected directly to the PC......................................................................................70
3.6 A CCESSORIES INSTALLATION ................................................................................................71
3.6.1 External pressure regulator installation (only for Headspace and SPME mode)....................71
3.6.2 Swagelok Adapter installation (only forHeadspace and SPME mode).......................................72
3.6.3 System integrity tool installation (only for Headspace mode).........................................................73
3.6.4 Vial Locator: Installing and removing (Liquid mode) ....................................................................75
3.7 P REPARING SAMPLE VIAL .....................................................................................................78
3.7.1 Vial filling in liquid mode ...............................................................................................................79
3.7.2 Vial capping (Screw Cap)...............................................................................................................79
3.7.3 Vial capping (Crimping Cap)..........................................................................................................79
3.8 F INAL INSTALLATION OPERATIONS .........................................................................................83
3.9 B ASIC TRAINING ..................................................................................................................83
4 SET UP OPERATIONS............................................................................................................84
4.1 V IEW P ARAMETERS .............................................................................................................84
4.2 S ETUP ...............................................................................................................................85
4.2.1 Setup: Alignment.............................................................................................................................85
4.2.2 Setup: Syringe warmer assembly installation/ replacement (Headspace mode)............................92
4.2.3 Syringe installation/ replacement (liquid mode)...........................................................................101
4.2.4 SPME Fiber holder installation/ replacement ..............................................................................111
4.2.5 Setup: Manual operations.............................................................................................................119
4.2.6 Setup: Configuration.....................................................................................................................123
4.2.7 Setup: Service................................................................................................................................135
5 PROGRAMMING METHOD...................................................................................................136
5.1 M ETHOD (H EADSPACE MODE ) ............................................................................................136
5.1.1 Method Menu................................................................................................................................136
5.1.2 Method X: Setting method parameters..........................................................................................136
5.1.3 Method tools..................................................................................................................................140
5.1.4 Example ........................................................................................................................................140
5.2 M ETHOD (L IQUID MODE ) ....................................................................................................141
5.2.1 Method Menu................................................................................................................................141
5.2.2 Method X: Setting method parameters..........................................................................................142
5.2.3 Method tools..................................................................................................................................146
5.2.4 Example ........................................................................................................................................146
5.3 M ETHOD : SPME MODE .....................................................................................................147

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5.3.1 Method Menu................................................................................................................................147
5.3.2 Method X: Setting method parameters..........................................................................................147
5.3.3 Method tools..................................................................................................................................153
5.3.4 Example ........................................................................................................................................153
6 PROGRAMMING SEQUENCE...............................................................................................155
6.1 S EQUENCE : H EADSPACE MODE ..........................................................................................155
6.1.1 Sequence Menu .............................................................................................................................155
6.1.2 Setting sequence step parameters .................................................................................................155
6.1.3 Sequence tools...............................................................................................................................156
6.2 S EQUENCE : L IQUID MODE ..................................................................................................157
6.2.1 Sequence Menu .............................................................................................................................157
6.2.2 Setting sequence step parameters .................................................................................................157
6.2.3 Sequence tools...............................................................................................................................159
6.3 S EQUENCE : SPME MODE ..................................................................................................159
6.3.1 Sequence Menu .............................................................................................................................159
6.3.2 Setting sequence step parameters .................................................................................................160
6.3.3 Sequence tools...............................................................................................................................161
7 OPERATIONS.......................................................................................................................162
7.1 H OME SCREEN ..................................................................................................................162
7.1.1 Home screen: Standard User Interface.........................................................................................162
7.1.2 Home screen: Quick Start User Interface.....................................................................................164
7.2 S INGLE INJECTION .............................................................................................................165
7.3 A UTOMATIC RUN ...............................................................................................................166
7.3.1 Interrupting an automatic run.......................................................................................................167
7.3.2 Immediate Injection.......................................................................................................................167
7.3.3 Skip Next Vial option.....................................................................................................................167
7.3.4 Priority injection...........................................................................................................................168
7.4 S YRINGE PURGE ...............................................................................................................169
7.5 S YRINGE WASH .................................................................................................................169
7.6 F IBER C LEANING /C ONDITIONING .........................................................................................170
7.7 L OAD AND U NLOAD S AMPLE V IAL .......................................................................................170
7.8 L OAD AND U NLOAD S OLVENT V IALS ....................................................................................171
7.9 S ETTINGS M ENU ...............................................................................................................171
7.9.1 Change tray/rack type...................................................................................................................171
7.9.2 Eco Savings...................................................................................................................................173
7.10 C HANGE M ODE .................................................................................................................174

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7.10.1 Change from Headspace to Liquid mode..................................................................................175
7.10.2 Change from SPME to Liquid mode .........................................................................................182
7.10.3 Change from Liquid to Headspace mode..................................................................................189
7.10.4 Change from Liquid to SPME mode .........................................................................................195
7.10.5 Change from Headspace to SPME mode..................................................................................201
7.10.6 Change from SPME to Headspace mode..................................................................................204
8 TROUBLESHOOTING...........................................................................................................207
8.1 I NTRODUCTION .................................................................................................................207
8.2 S TART UP ERRORS ............................................................................................................208
8.2.1 Safety lock error............................................................................................................................208
8.2.2 Vial locator error..........................................................................................................................208
8.2.3 Memory error................................................................................................................................209
8.3 M ETHOD ERRORS .............................................................................................................209
8.3.1 Fill Volume/Pull Up Strokes setting incompatibility.....................................................................209
8.3.2 Fill volume/Syringe Pre-Fill setting incompatibility.....................................................................210
8.3.3 Internal Standard setting error.....................................................................................................210
8.3.4 “Sample washes” setting error.....................................................................................................211
8.4 T OUCH S ENSOR PROBLEMS ...............................................................................................211
8.5 F ALLING VIALS ..................................................................................................................211
8.6 O BSTACLE FOUND .............................................................................................................212
8.7 O BSTACLE FOUND DURING COVER MOVEMENT .....................................................................213
8.8 L ACK OF A V IAL ( WASTE OR SOLVENT VIAL FOR LIQUID MODE , WASHING VIAL FOR SPME MODE ) 213
8.9 M ISMATCH ERRORS ...........................................................................................................214
8.9.1 Syringe wash mismatch error........................................................................................................214
8.9.2 Single injection mismatch error....................................................................................................214
8.9.3 Automatic run mismatch errors ....................................................................................................215
8.10 M ISSING ERRORS ..............................................................................................................217
8.10.1 Missing: rear injector alignment ..............................................................................................217
8.11 T IMEOUT ERROR ...............................................................................................................218
8.11.1 Peripheralcommunicationtimeout............................................................................................218
8.12 S YRINGE ID TIMEOUT .........................................................................................................218
8.13 M ECHANICAL ERRORS .......................................................................................................219
8.14 H EATING ERRORS .............................................................................................................219
8.15 S YSTEM INTEGRITY TEST FAIL .............................................................................................220
8.16 D AMAGE TO SYRINGE NEEDLE ............................................................................................220
8.16.1 Syringe inspection.....................................................................................................................221
8.17 T RAY EMERGENCY RELEASE ...............................................................................................222

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8.17.1 Tray opening by hand ...............................................................................................................222
8.18 “HTA A UTOSAMPLER M ANAGER ” TROUBLESHOOTING ...........................................................224
8.18.1 Autosampler and PC do not communicate (connection by LAN) ......................................224
8.19 A NALYTICAL TROUBLESHOOTING .........................................................................................226
8.19.1 Analytical troubleshooting (Headspace Mode).........................................................................226
8.19.2 Analytical troubleshooting (Liquid Mode)................................................................................228
8.20 B AR C ODE R EADER T ROUBLESHOOTING .............................................................................230
9 MAINTENANCE.....................................................................................................................232
9.1 O RDINARY MAINTENANCE ..................................................................................................232
9.1.1 Cleaning of vial rack/tray .............................................................................................................232
9.1.2 External cleaning of cabinet .........................................................................................................233
9.1.3 Incubation oven cleaning..............................................................................................................233
9.1.4 Touch screen display cleaning......................................................................................................234
9.1.5 Syringe replacement in the syringe warmer assembly (Headspace mode)...................................234
9.1.6 Fiber replacement in the Fiber holder (SPME mode)...................................................................241
9.1.7 Septum of the System integrity tool replacement (Headspace mode)............................................243
9.1.8 Preventive maintenance pack........................................................................................................243
9.2 E XTRAORDINARY MAINTENANCE .........................................................................................244
10 UNINSTALLING AND DISPOSAL ......................................................................................245
10.1 A UTOSAMPLER UNINSTALLING ............................................................................................245
10.2 A UTOSAMPLER SHIPPING ...................................................................................................246
10.3 A UTOSAMPLER DISPOSAL ...................................................................................................250
10.4 D ISPOSAL INSTRUCTIONS ...................................................................................................251
11 APPENDIX A – GLOSSARY ..............................................................................................252
11.1 H EADSPACEMODE ............................................................................................................252
11.2 L IQUIDMODE ....................................................................................................................253
11.1 SPME MODE ....................................................................................................................254
12 APPENDIX B – CONSUMABLES.......................................................................................256
12.1 C ONSUMABLES FOR H EADSPACE MODE ...............................................................................256
12.1.1 Syringes.....................................................................................................................................256
12.1.2 Sample vials..............................................................................................................................256
12.2 C ONSUMABLES FOR L IQUID MODE .......................................................................................260
12.2.1 Syringes.....................................................................................................................................260
12.2.2 Sample vials..............................................................................................................................263
12.2.3 Solvent and waste vials.............................................................................................................266

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12.3 C ONSUMABLES FOR SPME MODE .......................................................................................266
12.3.1 Fibers........................................................................................................................................266
12.3.2 Washing vial .............................................................................................................................266
13 APPENDIX C – ANALYZER CONNECTOR........................................................................267
13.1 RS232C INTERFACE ( OPTIONAL ) ........................................................................................269

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1 Introduction
1.1 Intended Use and Restrictions
HT2800T is an all-in-ones GC autosampler for static Headspace analysis, liquid sample injection
and SPME.
The autosampler must be used in a professional environment, and only by properly trained
users. The autosampler must be used according to the regulations in force regarding the safety
in testing laboratories.
A list (not exhaustive) of typical applications of the autosampler is reported below:
• Environmental analysis
• Quality control in the chemical and pharmaceutical industry
• Studies related to pharmaceuticals
• Drug discovery
Other techniques or areas of application are possible.
The safety measures and precautions regarding use, maintenance and repair, are not intended
for applications that require the use of toxic or infectious substances.
Every possible mixture or omission of a component cannot be recognized or previously
prevented. The operator that will use the autosampler must be trained about the used
substances, their hazards, their use and their proper disposal. For the correct use of the used
substances, refer to chemicals safety data sheets (MSDS) and to the regulations, laws,
directives and safety decrees currently in force that regulate the behaviour inside the testing
laboratories.
The insertion and positioning of the sample (vial), the insertion of syringes and all the other
preparation activities are under the sole responsibility of the user. The user must operate in
accordance with the law, in particular the ones concerning the safety of testing laboratories and
accident prevention.

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The operator must be trained to operate the equipment in accordance with the safety standards
for the laboratories. The autosampler does not have specific protection mechanisms against
substances that are corrosive, potentially infectious, toxic and radioactive or against other
substances that can be hazardous to health. However, when the autosampler is used, because
of the handled substances, the user could need to use individual protection measures such as
gloves, masks and goggles; the use of such devices must comply with the regulations currently
in force regarding the safety of testing laboratories.
The use of the autosampler for any technique invasive for humans or animals is explicitly
prohibited. The autosampler must be used only in the manner described in the usage
instructions. Any other use may affect the autosampler and operator safety.
HTA s.r.l. is not responsible for damage caused, even only in part, by improper use of the
autosampler, by unauthorized modification of it, by different or missing execution of the
procedures described in this manual, or by the use of the autosampler violating the applicable
laws and regulations.
The autosampler has been designed and tested in accordance with current safety standards.
1.2 Symbol Table
Please carefully read this table before using your autosampler. These symbols are used in this
manual.
Do not touch: action forbidden
Warning: check the manual
High voltage
Warning: indicates pinch, crush and cut hazard
Warning: indicates the possible presence of biological or hazardous substances
Hot surface