Version 2017-01-19 for GI use
In case the alignment of the microscope is lost, which will for example become
evident because the beam has an oval shape (astigmatism), the original settings can
be recalled. Ask assistance. [Note for assistants: to the hardware keyboard (the one
going along with the small monitor), press ESC to make sure to start with a clean line
and type: ufc o 1 (which is ufc in small letters, space, letter o , not zero, in small font,
space and then 1). Press Enter. ]
To acquire an image, press on the right camera icon of intelligent exposure. The
entire gray levels displayed on the histogram are automatically recorded. However,
the brightness settings should be adjusted in such a way that the histogram is
centered and as broad as possible so that many gray levels are recorded, but also
the exposure time should be as short as possible, in order to prevent vibrations to
bias the image quality. Note that there is a slight difference in eveness ofillumination
for direct vision under the column or through the camera (use brightness to
compensate).
In case automatic balancing of the
camera is not suitable, for example
because the interesting parts in the
specimen are very dark or very bright,
one can apply (automatic, fixed scale or
manual) exposure on a Region of
Interest around these part (click the
green screen icon within the Intelligent
exposure pop-up window and drag a
ROI). Manual exposure, manual
minimum and maximum setting and
manual gamma adjustment is also possible. Ask Geert-Jan for the various options.
For saving images, enter the correct magnification (mag x can be read on the small
hardware monitor) and image name in the own folder (D:>Users> path). Scale bars
are automatically burned into the image. (Cancel the second pop-up screen). Images
can be uploaded to a server at the end of a session.
To finish a session, go back to small magnification (lower diaphragm handle to the
right). Finish after having taken one picture to allow the charge on the chip of the
camera to be withdrawn and switch of the filament 2.
To unload the specimen holder, always check first (once again) that the FILAMENT
IS OUT (!). Gently pull the rod to the final stop. Turn one quarter anti-clockwise.
Release the rod by holding one finger against the goniometer to counter the vacuum
sucking in a controlled manner and pull the rod out. If you are the last user of the day,
switch also the HT 1out. Exit the program.
Place the grid rod holder on the standard and release the grid cover with the wing
key. Allow the grid to fall on a clean piece of paper and lift it with tweezers to set it
back in the grid box. Close the cover and secure the wing key in its cavity.
HALIGNMENT JEOL 1010 TEM (GI)
HOW TO CHECK THE SATURATION OF THE TEM FILAMENT?
1. Start the High Tension (HT) and the filament (Fil)
