Jouan SH 105 User manual

MEDIA PREPARATORS
SH105/110
USER’S MANUAL
25529080-b

CAREFULLY READ THIS MANUAL BEFORE OPERATING YOUR INSTRUMENT.
INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF JOUAN ;
IT MAY NOT BE DUPLICATED OR DISTRIBUTED WITHOUT THE OWNER’S AUTHORIZATION.
THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF THE INSTRUCTIONS
AND PRECAUTIONS DESCRIBED IN THIS DOCUMENT
MEDIA PREPARATORS
SH105/110
USER’S MANUAL
25529080-b

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REVISION STATUS
INDEX DATE AMENDED PAGES NOTES
-11/89 Initial release
-01/90 5, 9, 12, 19, Fig. 3 Handle and F5
a06/93 6.3, Figs. 4 & 5 Safety valve
b08/02 Revised edition

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GUARANTEE TERMS
JOUAN guarantees that this unit is free from defects in materials and workmanship when it leaves the factory, and
undertakes to replace or repair the unit if it proves defective in normal use or during servicing for a period of
ONE YEAR from the delivery.
Our liability under this guarantee is limited to repairing the defective unit or any part of the unit providing it is
sent, carriage paid, to an authorized service centre or the SAINT-HERBLAIN office.
This guarantee is invalidated if the unit is incorrectly used, poorly serviced or neglected, mis-used or
accidentally damaged.
There is no explicit guarantee other than as stated above.
FOR FURTHER INFORMATION, ASSISTANCE OR SERVICING :
USA/ Canada : JOUAN Inc.: JOUAN Global Center, 170 Marcel Drive, WINCHESTER, VIRGINIA
Tel. [phone] - Fax. [phone] - e-mail : [email protected]
U.K. : JOUAN Ltd: Merlin Way, Quarry Hill Road, ILKESTON, DERBYS, DE7 4RA
Tel. [phone] - Fax. [phone] - e-mail : [email protected]
France : JOUAN S.A.: 10, rue Duguay Trouin, 44807 SAINT HERBLAIN Cedex
Tel. [phone] - Fax. [phone] - e-mail : [email protected]
Germany : JOUAN GmbH: Kapellenstrasse 22, 82008 UNTERHACHING
Tel. [phone] - Fax. [phone] - e-mail : [email protected]
Netherlands : JOUAN Nederland B.V.: Frans Halslaan 23d, 1231 BB LOOSDRECHT
Tel. [phone] - Fax. [phone] - e-mail : [email protected]
Nordic Region : JOUAN Nordic A/S: Gydevang 17-19, 3450 ALLERØD, Denmark
Tel. [phone] - Fax. [phone] - e-mail: [email protected]
Italy : JOUAN Italia: Via Carlo Porta 3, 20093 COLOGNO MONZESE (MI)
Tel [phone] - Fax [phone] - e-mail : [email protected]
Russia : JOUAN Moscow: CGSEN JOUAN Office, 5/14 Timiryazevskaya Str. Office 50-52, 125422 MOSCOW
Tel. [phone] - Fax. [phone] - e-mail : [email protected]
Ukraine : JOUAN Ukrainia: Leontovitch Str. 9, KIEV 30, 01030 UKRAINE
Fax/Tel [phone] - e-mail : [email protected]
China : JOUAN China: JOUAN Suzhou Office, Room 404-405 SIBI, 233# Binhe road, SUZHOU, P.C. 215011
Tel [phone] - Fax [phone] - e-mail : [email protected]
Other Countries: JOUAN S.A.: Service Assistance Technique
10, rue Duguay Trouin, 44807 SAINT HERBLAIN Cedex
Tel. [phone] - Fax. [phone] - e-mail : [email protected]
Website : www.jouan.com

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CHAPTER 1 - USE AND FUNCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.1. Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.2. Fluids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.3. Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
CHAPTER 2 - INSTALLATION PROCEDURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.1. Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2. Lifting and transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.3. Work surface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.4. Environmental conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.5. Electrical connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.6. Water connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
CHAPITRE 3 - SPECIFICATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1. Weight and dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2 Mains electricity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.3 Water supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.4 Capacity by volume of media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.5 Mixing speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.6 Chamber specification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.7 Timing of standard processing cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.8 Temperature control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.9 Cycle timing control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.10 Overpressure valve settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
CHAPITRE 4 - INSTRUCTIONS FOR USE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1. Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.2. Setting up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
4.3 Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
4.4 Voluntary or involuntary interruption of the process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
4.5 Maintenance after use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
CHAPITRE 5 - HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1. Risks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.2. Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.3 Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
CHAPITRE 6 - SERVICING AND PREVENTATIVE MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.1. Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.2. Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.3. Spare parts list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-2
CONTENTS

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1USE AND FUNCTION
1.1. OVERVIEW
The SH105 and SH110 media preparators are automatic units for the preparation of culture media. They
mix the constituents (water - dehydrated ingredients - additives . . .) and also sterilise and cool the me-
dium according to data supplied by the operator.
Many types of agar can be prepared including delicate ones such as heated blood-agar and fresh blood-
agar.
FIG. 1.1 - VIEW OF THE SH 110

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1.2. FLUIDS
The fluids contained in the bowl must not be:
-explosive,
-comburants,
-inflamable,
-toxic.
All the fluids used must belong to the group 2 of the European Pressure Directive 97/23 CE.
Fluid used in the heating chamber (external circuit) = water (see Chapter 2.5).
1.3. ACCESSORIES
The SH105 - SH110 are supplied complete with the following accessories :
-1set spare fuses,
- 2 sets water connecting pipes,
- 2 sets Serflex hose couplings,
- 1 set seals for chamber and lid,
- 2 dispensing port nipples with silicon tubing,
- 2 dispensing port locking cap,
- 1 unlocking tool for safety cover,
- 1 chamber cover stand,
- 1 paper roll for units fitted with recorder.

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2 - 1
2.1. UNPACKING
Check that the parts on the packing list are included in the package. If any parts are missing, contact your
JOUAN agent.
Remove all accessories and paperwork.
2.2. LIFTING AND TRANSPORT
Due to the weight of the machine, all lifting and transporting must be carried out using proper handling
equipment (eg : fork lift trolley) that complies with current regulations, and by people having undergone
the necessary training.
The machine must be supported from underneath. If it has to be transported without its pallet, for example
on a staircase, professional handling assistance is required.
Carefully lift the unit by holding it at the sides.
Never attempt to lift the unit by the front, the cover or the pipes coming out of the back.
2.3. WORK SURFACE
Place the media preparator on a work surface that does not produce vibrations.
This surface must be at a height of about 0.850 m to facilitate working conditions, and close to a water and
electricity supply. It must be able to support the weight of the unit under working conditions (+8 kg for the
SH105 ; +14 kg for the SH110).
2.4. ENVIRONMENTAL CONDITIONS
This instrument is designed to operate safely under the following conditions:
-Indoor use.
-Temperature : 5° to 40°C.
-Maximum relative humidity of 80% for temperatures up to 22°C.
-Maximum altitude : 2000 m.
Maximum performance is assured across the following temperature range : 15°C to 25°C.
This instrument is not designed to resist an earthquake.
2INSTALLATION PROCEDURE

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2.5. ELECTRICAL CONNECTION
Connect the supply cable to the mains.
The electricity supply must be single-phase, 1 live, 1 neutral and 1 earth.
The SH105 requires 10 A at 220 - 240 V A.C. (50 or 60 Hz depending on type).
The SH110 requires more power : 13 A at 220 - 240 V.
The unit must be earthed for personal safety.
The SH105/SH110 media preparators have a built-in mains cable.
Remember that in order to respect the electrical safety standards related to protection against indirect
contact, the supply of power to the instrument must be via a power socket fitted with a protection device
ensuring automatic cut-off in the case of an insulation fault.
A supply fitted with a circuit breaker of the correct rating complies with this requirement.
2.6. WATER CONNECTION
The unit is supplied complete with connecting tubes and couplings. Connections are made at the rear of
the unit.
FIG. 2.1 - WATER CONNECTION

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1. Water inlet tubing
2. Water outlet tubing
3. Pressure regulator
4. Water level detector
5. Steam condensor
2.6.1 WATER INLET
The water supply pressure must be at least 2 bars. The water supply temperature must be between 10°C
and 30°C if the unit is to work properly.
The supply tube is connected to the lower port (point 1 and 3) with a hose coupling to ensure a water-tight
connection.
2.6.2 WATER OUTLET
Despite the inclusion of a steam condenser in the hydraulic circuit outlet, a water discharge connection
capable of withstanding boiling water under pressure is required. The discharge pipe is connected to the
upper port (point 5) at the rear of the unit with a clamp.
Care must be taken to ensure that the other end of the discharge pipe is located so as to prevent steam
under pressure being released into the room.