Knauer Azura pH 2.1S User manual

pH Monitor pH 2.1S
Instructions
Document No. V6733
HPLCHPLC

Note: For your own safety, read the instructions and observe the
warnings and safety information on the device and in the instructions.
Keep the instructions for future reference.
Note: In case you require this instruction in another language, please
submit your request including the corresponding document number via
e-mail or fax to KNAUER.
Do you have questions about the installation or the operation of your
instrument or software?
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Contact your local KNAUER partner for support:
www.knauer.net/en/Support/Distributors-worldwide
Support in Germany
(Austria & Switzerland on case-to-case basis):
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KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Germany
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-mail: [e-mail]
Document number: V6733
Version number: 1.0
Release date: February 07, 2023
Translation of the original edition
These instructions apply to devices with the product number:
EDG31
The information in this document is subject to change
without prior notice. For the latest version of the instructions, visit our
website: www.knauer.net/library
The printed versions of our instructions are printed according to
Blue Angel standards (www.blauer-engel.de/en/uz195).
This document contains confidential information and may not be
reproduced without written consent of KNAUER Wissenschaftliche
Geräte GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2023
All rights reserved.
AZURA® is a registered trademark of
KNAUER Wissenschaftliche Geräte GmbH.
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AZURA® pH Monitor pH 2.1S Instructions, V6733
Table of contents
1. General ..............................................................................1
1.1 About these instructions..........................................................1
1.2 Signal words ....................................................................1
1.3 Additional typographical conventions ..............................................1
1.4 Legal information................................................................2
1.4.1 Liability limitation .........................................................2
1.4.2 Transport damage ........................................................2
1.4.3 Warranty conditions .......................................................2
1.4.4 Warranty seal .............................................................2
1.4.5 Declaration of conformity ..................................................2
2. Basic safety instructions ................................................................3
2.1 Intended use....................................................................3
2.1.1 Operating ranges .........................................................3
2.1.2 Foreseeable misuse .......................................................3
2.2 User qualification ................................................................3
2.3 Operator responsibility ...........................................................4
2.4 Personal safety equipment ........................................................4
2.5 Specific environments............................................................4
2.5.1 Earthquake-endangered areas..............................................4
2.5.2 Explosive environment.....................................................5
2.5.3 Cooling room ............................................................5
2.5.4 Wet room ................................................................5
2.6 Maintenance, care and repair .....................................................5
2.7 Service request form and decontamination report ...................................5
3. Product information ...................................................................6
3.1 Features ........................................................................6
3.2 Device views ....................................................................6
3.3 Symbols and signs ...............................................................7
3.4 Scope of delivery ................................................................7
3.5 Eluents/buers ..................................................................8
3.5.1 Suitable eluents/buers....................................................8
3.5.2 Unsuitable eluents/buers .................................................8
4. Installation............................................................................9
4.1 Setup ..........................................................................9

AZURA® pH Monitor pH 2.1S Instructions, V6733
4.1.1 Location requirements .....................................................9
4.1.2 Unpacking ..............................................................10
4.1.3 Integration into the FPLC/HPLC System .....................................10
4.2 AZURA® pH sensor .............................................................11
4.2.1 Implementing the AZURA® pH flow cell intothechromatography system........11
4.2.2 Assembling pH sensor and attaching to pH monitor AZURA® pH 2.1S...........12
4.3 Connecting the device to the computer ...........................................13
4.3.1 Connecting the device to the computer via LAN .............................13
4.3.2 Configuring the LAN settings ..............................................13
4.3.3 Connecting devices to the LAN ............................................14
4.3.4 Configuring the router ....................................................15
4.3.5 Integrating the LAN into a company network ................................15
4.3.6 Controlling several systems separately in a LAN..............................15
4.4 Setting IP addresses via software .................................................16
4.4.1 Firmware Wizard: Setting a static IP address .................................16
4.4.2 Firmware Wizard: Setting a dynamic IP address ..............................17
4.5 Power connection ..............................................................17
5. Operation ...........................................................................19
5.1 Initial start-up ..................................................................19
5.2 Switch on ......................................................................19
5.2.1 Operating elements ......................................................19
5.2.2 Menu items and factory settings ...........................................20
5.3 Selecting the interface ..........................................................20
5.4 Checking the operating hours....................................................21
5.5 Calibrating the pH electrode .....................................................21
5.6 Switching o device ............................................................23
6. Functionality tests ....................................................................24
6.1 Installation Qualification (IQ) .....................................................24
6.2 Operation Qualification (OQ) ....................................................24
7. Troubleshooting......................................................................25
7.1 LAN...........................................................................25
7.2 Possible problems and solutions..................................................26
8. Maintenance and care ................................................................27
8.1 Maintenance contract ...........................................................27
8.2 Cleaning the device.............................................................27
8.3 Flushing and cleaning the pH sensor ..............................................27

AZURA® pH Monitor pH 2.1S Instructions, V6733
8.3.1 Cleaning pH electrode....................................................27
8.3.2 Cleaning pH flow cell .....................................................28
8.4 Replacing pH electrode .........................................................28
9. Transportation and storage ............................................................29
9.1 Preparing wetted components ...................................................29
9.2 Disassembling the device........................................................29
9.2.1 Disconnecting the power supply ...........................................29
9.2.2 Removing the pH sensor ..................................................29
9.3 Storing the device ..............................................................30
9.3.1 Storage of the pH monitor ................................................30
9.3.2 Storage of the pH sensor..................................................30
10. Disposal.............................................................................31
10.1 AVV marking in Germany ........................................................31
10.2 WEEE registration ..............................................................31
10.3 Solvents and other operating materials ............................................31
11. Technical data........................................................................32
12. Chemical compatibility of wetted materials..............................................33
12.1 General .......................................................................33
12.2 Plastics ........................................................................33
12.3 Non-metals ....................................................................35
12.4 Metals.........................................................................36
13. Repeat orders........................................................................37

AZURA® pH Monitor pH 2.1S Instructions, V6733
1
1. General
1.1 About these instructions
These operating instructions enable the safe and ecient operation of
the device. The user must have carefully read and understood these
operating instructions before starting any work.
The basic prerequisite for safe operation is compliance with all safety
instructions (see chapter “2 Basic safety instructions”, p. 3). In addition
to the safety and warning instructions in these operating instructions, the
local accident prevention regulations and the national industrial safety
regulations apply.
These operating instructions are an integral part of the device. It must be
kept in the immediate vicinity of the device and accessible to the user at
all times.
You can download these and other instructions from the KNAUER web-
site: www.knauer.net/library
1.2 Signal words
Possible dangers related to the device are distinguished in personal and
material damages.
Symbol Meaning
DANGER (red) indicates a highly hazardous situa-
tion. If not avoided, it will result in death or serious
injury.
WARNING (orange) indicates a hazardous situa-
tion. If not avoided, it could result in death or
serious injury.
CAUTION (yellow) indicates a moderate hazardous
situation. If not avoided, it could result in minor or
moderate injury.
NOTICE (blue) is used to address issues which are
not related to physical injury.
1.3 Additional typographical conventions
Note: Specific information are prefixed with the word “Note” and an
information icon.
Note: This is an example.

2 General
AZURA® pH Monitor pH 2.1S Instructions, V6733
1.4 Legal information
1.4.1 Liability limitation
The manufacturer is not liable for the following issues:
Non-compliance of these instructions
Non-observance of necessary safety precautions
Improper use
Operation of the device by unqualified personnel (see chapter “2.2
User qualification”, p. 3)
Use of non-approved spare parts
Technical changes by the user such as opening the device and unau-
thorized modifications
Violations of General Terms and Conditions (GTC)
1.4.2 Transport damage
The packaging of our devices provides the best possible protection
against transport damage. However, check the packaging for transport
damage. In case you notice any damage, inform the technical support
and the shipping company within three workdays.
1.4.3 Warranty conditions
For information on warranty please refer to our general terms and condi-
tions on the website: www.knauer.net/terms
1.4.4 Warranty seal
A blue or orange warranty seal is axed to some devices.
A blue seal is used by KNAUER’s Manufacturing or Customer Support
for devices to be sold.
After repair, service technicians attach an orange seal onto the identi-
cal position.
After repair, the service technician axes an orange seal in the same
place. If unauthorised persons tamper with the device or if the seal is
damaged, the warranty will lapse.
1.4.5 Declaration of conformity
The declaration of conformity is enclosed as a separate document with
the product and can be obtained online:
www.knauer.net/en/Support/Declarations-of-conformity.

AZURA® pH Monitor pH 2.1S Instructions, V6733
3
2. Basic safety instructions
The device has been developed and constructed in such a way that haz-
ards arising from its intended use are largely excluded. Nevertheless, the
following safety instructions must be observed in order to exclude residu-
al hazards.
2.1 Intended use
Note: Only use the device for applications that fall within the range of
the intended use. Otherwise, the protective and safety equipment of the
device could fail.
The pH monitor pH 2.1S can be connected to an pH electrode and fitting
pH flow cell in order to measure the pH in aqueous buers. The monitor
itself is the processing device and only functional in combination with a
pH electrode and flow cell.
The monitor is used for pH monitoring in the following areas:
Biochromatography
Process analysis
Biochemical analysis
Pharmaceutical analysis
Environmental analysis
2.1.1 Operating ranges
The device is intended to be used indoors for chromatographic
applications.
2.1.2 Foreseeable misuse
Refrain from the use of the device for the following purposes or
conditions:
Medical purposes. The device is not approved as a medical product.
Operating outdoors. Otherwise, the manufacturer does not guarantee
the functionality and safety of the device.
Operation in potentially explosive areas without special and addition-
al explosion protection. Contact the KNAUER Customer Support for
more information.
2.2 User qualification
The users are qualified to handle the device if all of the following points
apply:
They have at least a basic knowledge of liquid chromatography.
They have knowledge about the properties of the used solvents and
their health risks.
They are trained for the special tasks and activities in the laboratory
and know the relevant standards and regulations.
Due to their technical training and experience, they can under-
stand and carry out all the work described in the operating instruc-
Description
Operating Range

4 Basic safety instructions
AZURA® pH Monitor pH 2.1S Instructions, V6733
tions on the instrument and recognize and avoid possible dangers
independently.
Their ability to react is not impaired by the consumption of drugs,
alcohol or medication.
They have participated in the installation of an instrument or training
by KNAUER or an authorized company.
If users do not meet these qualifications, they must inform their
supervisors.
2.3 Operator responsibility
The operator is any person who operates the device himself or leaves it to
a third party for use and who bears the legal product responsibility for the
protection of the user or third parties during operation.
The obligations of the operator are listed below:
Know and follow the applicable work safety regulations
Identify hazards arising from the working conditions at the place of use
in a risk assessment.
Set up operating instructions for the operation of the device.
Regularly check whether the operating instructions correspond to the
current status of the regulations.
Clearly regulate and specify responsibilities for installation, operation,
troubleshooting, maintenance and cleaning and set clear rules
Ensure that all personnel who work with the device have read and
understood these operating instructions
Train the personnel who work with the device at regular intervals and
inform them about the dangers.
Provide the necessary safety equipment to the employees working
with the unit (see section below).
2.4 Personal safety equipment
The protective measures required in the laboratory must be observed
and the following protective clothing worn during all work on the device:
Safety glasses with side protection
Protective gloves in accordance with the prevailing ambient conditions
and used solvents (e.g. heat, cold, protection against chemicals)
Lab coat
Personalised protective safety equipment which is specified in the
particular laboratory.
2.5 Specific environments
2.5.1 Earthquake-endangered areas
In earthquake-endangered areas, do not stack more than 3 devices on
top of each other. Otherwise there is risk of injury due to falling devices
or loose parts.

Basic safety instructions 5
AZURA® pH Monitor pH 2.1S Instructions, V6733
2.5.2 Explosive environment
Never use the system in potentially explosive atmospheres without
appropriate protective equipment. For more information, contact the
KNAUER Customer Support.
2.5.3 Cooling room
You may operate the device in a cooling room. To prevent condensation,
note the following instructions:
Allow the device to acclimatize for min. 3 hours before taking it into
operation.
After taking into operation, the device should stay switched on.
Avoid temperature fluctuations.
2.5.4 Wet room
The device must not be operated in wet rooms.
2.6 Maintenance, care and repair
Avoiding electric shock: Before performing any maintenance and ser-
vice work, disconnect the device from the power supply.
Tools: Use only tools recommended or prescribed by the
manufacturer.
Spare parts and accessories: Only use original parts and accessories
made by KNAUER or a company authorized by KNAUER.
PEEK fittings: Use PEEK fittings only for a single port or brand-new
PEEK fittings in order to avoid dead volume or not exactly fitting
connections.
Column care: Follow KNAUER or other manufacturer’s instructions on
caring for the columns (see www.knauer.net/columncare)
Used capillaries: Do not use any used capillaries elsewhere in the
system in order to avoid dead volumes, not exactly fitting connections
and spreading contamination.
Safety features: The device may only be opened by the KNAUER Cus-
tomer Support of KNAUER or any company authorized by KNAUER
(see chapter “1.4.1 Liability limitation”, p. 2).
For more information visit the KNAUER website:
www.knauer.net/hplc-troubleshooting
2.7 Service request form and decontamination
report
Devices which are shipped without the completed document “Service
request form and decontamination report” will not be repaired. If you
would like to return a device to KNAUER, make sure to enclose the com-
pleted document: www.knauer.net/servicerequest.