Knauer BlueShadow 50D Operating instructions

Detector 50D
Service Instructions
Document Number: V6700
Document No. VSM-021
HPLCHPLC
BlueShadow

Note: For your own safety, read the instructions and follow the
warnings and safety information on the device and in the instructions.
Keep the instructions for future reference.
Telefon: [phone] (9-17h, Central European Time)
Fax: [phone]
E-Mail: [e-mail]
Languages: German, English
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Telefon: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [e-mail]
Document number: VSM-021
Version number: 1.0
Release date: 2021/02/24
Original edition
The information in this document is subject
to change without prior notice.
For the latest version of the service instruction, visit the
partner area on our website (login required):
www.knauer.net/Service-instructions
This document contains confidential information and may not be
reproduced without written consent of KNAUER Wissenschaftliche
Geräte GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2021
All rights reserved.
Customer
Support:
Publisher:
Version information:
Copyright:

i
BlueShadow® Detector 50D Service Instructions, VSM-021
Table of Contents
1. General ..............................................................................2
1.1 About these service instructions ...................................................2
1.2 Signal words ....................................................................2
1.3 Additional typographical conventions ..............................................3
1.4 Legal information ................................................................3
1.4.1 Liability limitation......................................................3
1.4.2 Warranty conditions ...................................................3
1.4.3 Declaration of conformity...............................................3
2. Safety instructions and authorization for service technicians ................................4
2.1 Qualification of the service technician ..............................................4
2.2 Service request form and decontamination report ...................................5
3. Protective measures ...................................................................6
4. What is maintained or repaired? ........................................................7
5. Disassembling the housing .............................................................8
5.1 Removing the device hood .......................................................8
5.2 Overview of the device ...........................................................9
6. Replacing the display .................................................................10
7. Replacing the keypad .................................................................12
7.1 Removing the keypad ...........................................................12
7.2 Installing a new keypad..........................................................13
8. Replacing the lamps ..................................................................14
9. Replacing the fan.....................................................................15
9.1 Removing the fan...............................................................15
9.2 Inserting a new fan..............................................................16
10. Replacing the mainboard..............................................................18
10.1 Procedure for replacement of the mainboard ......................................19
10.1.1 Configurating the plug connectors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
10.1.3 Unlocking flexible flat cable (signal and reference channel) ................20
10.1.4 Replacing fuses on the mainboard ......................................21

BlueShadow® Detector 50D Service Instructions, VSM-021
Table of Contents ii
11. Repairing the optical bench ...........................................................22
11.1 Repair measures................................................................22
11.1.1 Components of the optical bench ......................................22
11.1.2 Entrance diaphragm ..................................................23
11.2 Adjusting grating unit ...........................................................23
11.3 Optical light path ...............................................................24
11.4 Replacing the filter wheel drive...................................................25
11.4.1 Removing cable plug from the mainboard ...............................26
11.4.2 Removing filter wheel drive ............................................27
11.4.3 Installing filter wheel drive .............................................28
11.4.4 Inserting the filter wheel drive and reconnecting the cable plug
onto the mainboard ..................................................28
11.5 Removing timing belt ...........................................................30
11.5.1 Installing timing belt ..................................................30
11.6 Tightening the timing belt .......................................................32
11.7 Replacing the signal board ......................................................33
11.7.1 Removing the signal board ............................................35
11.7.2 Installing signal board.................................................36
11.7.3 Installing the flow cell .................................................37
11.7.4 Installing the flexible flat cable .........................................38
11.8 Replacing the reference board ...................................................40
11.8.1 Preparatory actions ...................................................40
11.8.2 Removing optical bench...............................................40
11.8.3 Removing the reference board .........................................44
11.8.4 Installing the reference board ..........................................45
11.9 Replacing the mirrors ...........................................................46
11.9.1 Removing the plane mirror (halogen lamp housing).......................46
11.9.2 Inserting the plane mirror (halogen lamp housing) ........................47
11.9.3 Cleaning lens of the halogen and deuterium lamp housing ................48
11.9.4 Replacing the plane concave mirror of the collimator .....................49
11.9.5 Replacing the concave mirror ..........................................50
11.9.6 Replacing plane mirror (main part of the optical bench) ...................51
11.9.7 Replacing concave mirror (main part of the optical bench) .................51
12. Removing the D2 power supply .......................................................52
13. Troubleshooting......................................................................53
13.1 Possible problems and repair solutions ............................................53
13.2 System messages...............................................................54
13.3 Error list .......................................................................55

BlueShadow® Detector 50D Service Instructions, VSM-021
Table of Contents iii
14. Spare parts ..........................................................................57
14.1 Caution Labels .................................................................57
14.2 Fan ...........................................................................57
14.3 Fuses .........................................................................58
14.4 Housing .......................................................................58
14.5 Lamps.........................................................................59
14.6 Mainboard and cabling..........................................................59
14.7 Optical bench..................................................................60
14.8 Power supply for the deuterium lamp .............................................63
14.9 Display and keypad .............................................................63

BlueShadow® Detector 50D Service Instructions, VSM-021
2
1. General
1.1 About these service instructions
This document is an addition to the user instruction of the device. It inclu-
des all work that requires the opening of the device, installation of certain
components and maintenance work.
The service instructions enable the safe and ecient operation of the
device. The service technician must have carefully read and understood
this service instructions before starting any work.
The basic prerequisite for safe operation is compliance with all safety
instructions (see chapter „2. Safety instructions and authorization for
service technicians“ on page 4). In addition to the safety and warning
instructions in these service instructions, the local accident prevention
regulations and the national industrial safety regulations apply.
Observe the following:
All safety instructions in the service instructions
All safety instructions in the user instructions
The environmental, installation and connection specifications in the
user instructions
Only use spare parts, tools and cleaning agents recommended or
prescribed by KNAUER.
Observe the RoHS conformity for mainboards! Never perform mainte-
nance on the mainboards independently. Always replace the mainboards
and return the defective boards to KNAUER technical support, otherwise
the warranty will lapse.
1.2 Signal words
Possible dangers related to the device are distinguished in personal and
material damages.
Symbol Meaning
DANGER (red) indicates a highly hazardous situa-
tion. If not avoided, it will result in death or serious
injury.
WARNING (orange) indicates a hazardous situation.
If not avoided, it could result in death or serious
injury.
CAUTION (yellow) indicates a moderate hazardous
situation. If not avoided, it could result in minor or
moderate injury.
NOTICE (blue) is used to address issues which are
not related to physical injury.
What must be taken
into account?
Do not perform repairs
on the mainboard

General 3
BlueShadow® Detector 50D Service Instructions, VSM-021
1.3 Additional typographical conventions
General equal treatment: When persons are described, this document
uses the male grammar form to keep the text easy to read. The form
has a neutral sense and speaks to people of any gender in the same
way.
Note: Specific information are prefixed with the word “Note” and an
information icon.
Note: This is an example.
1.4 Legal information
1.4.1 Liability limitation
The manufacturer is not liable for the following issues:
Non-compliance of these instructions
Non-observance of necessary safety precautions
Improper use
Operation of the device by unqualified personnel (see chapter
„2.1 Qualification of the service technician“ on page 4)
Use of non-approved spare parts
Technical changes by the service technician such as unauthorized
modifications
Violations of General Terms and Conditions (GTC)
1.4.2 Warranty conditions
For information on warranty please refer to our general terms and
conditions on the website: https://www.knauer.net/terms.
1.4.3 Declaration of conformity
The declaration of conformity is enclosed as a separate document with
the product and can be obtained online:
www.knauer.net/en/Support/Declarations-of-conformity.

BlueShadow® Detector 50D Service Instructions, VSM-021
4
2. Safety instructions and authorization for
service technicians
The device has been developed and constructed in such a way that
hazards arising from its intended use are largely excluded. Nevertheless,
the following safety instructions must be observed in addition to the
basic safety instructions in the user instructions in order to avoid residual
hazards.
The following applies for all repairs on the device:
Electric shock
Danger of electric shock from voltage-carrying parts inside the
device. The housing serves as a protective cover against voltages
inside the device.
ÎSwitch the device o before opening the device.
ÎPull the power plug.
2.1 Qualification of the service technician
These service instructions are intended for the following professional
groups:
Task Authorized
Maintenance KNAUER service technicians
Service technicians authorized by KNAUER
who have completed a service training
Level 2
Repair KNAUER service technicians
Service technicians authorized by KNAUER
who have completed a service training
Level 3
If you do not belong to one of these professional groups, you must
under no circumstances carry out the work described in this instruction.
The service technician is qualified to handle the device if all of the
following points apply:
Fluent English to be able to use the English-language service software
and online help.
Knowledge about the properties of the used solvents and their health
risks.
Protective measures
against any dangers
Electronic defect
Electrostatic discharge can destroy the electronics.
ÎWear a protective bracelet against electrostatic discharge and ground.
Who may perform
repairs?

Safety instructions and authorization for service technicians 5
BlueShadow® Detector 50D Service Instructions, VSM-021
Due to technical training and experience, the service technician can
understand and carry out all the work described in the service instruc-
tions and recognize and avoid possible dangers independently.
The ability of the technician to react is not aected by the consumption
of drugs, alcohol or medication.
2.2 Service request form and decontamination
report
We advise you not to repair devices which are not accompanied by the
completed document “Service request form and decontamination report”
(www.knauer.net/decontamination-report).

6
BlueShadow® Detector 50D Service Instructions, VSM-021
3. Protective measures
The following applies for all repairs of the device:
Symbols Warning instructions
DANGER!
High voltage can be lethal!
Never replace the lamps of the detector during operation!
High voltages can build up in the detector.
Switch o the device and pull the power plug to completely isolate it
from the supply voltage.
Danger of electric shock!
Switch o device or power supply! Pull the power plug!
WARNING!
Potential contamination of device through toxic, corrosive or radioacti-
ve substances.
The customer must ensure that the device has been thoroughly decon-
taminated before repairs are performed.
WARNING!
Danger of burns caused by hot (200 °C) deuterium or halogen lamps
inside detectors!
Allow the device to cool down for at least 15 minutes before opening!
WARNING!
Irritation of eye cornea through UV light! Exposure to concentrated
UV light from optic fibers can lead to irritation of the cornea.
Switch o the device and pull the power plug!
Caution!
Micro-electronic device components that could be damaged by
electro-static discharge (ESD) when touched.
Take precautions against electrostatic discharge.

BlueShadow® Detector 50D Service Instructions, VSM-021
7
4. What is maintained or repaired?
Hours of
device
operation
Procedures
1 000 Check the operating time and light intensity of the halogen lamp and
replace the lamp if necessary.
2 000 Check the operating time and light intensity of the D2 lamp and replace
the lamp if necessary.
Check the installation of the flow cell and clean the window of the flow
cell.
Check the tension of the tooth belt.
Check motor and axial tolerance.
Check the ratio of the light path for the signal and reference using the
service tool using a test cell, except for optical fiber detectors.
5 000 Check that the filter wheel is free of dust; order sorting filter and hol-
mium filter.
Check the tension of the tooth belt.
Check motor and axial tolerance.
Check that the silica glass plate of the beam splitter is clean.
10 000 Check that the mirror is free of dust.
Check the linearity with the service tool.
Check motor and axial tolerance.
Check the settings of all parameters with the service tool.
Check the actual number of operating hours of the motor using the ser-
vice tool. If 10 000 operating hours have actually been reached by the
motor, then it is important that you replace the tooth belt.

8
BlueShadow® Detector 50D Service Instructions, VSM-021
5. Disassembling the housing
It is necessary to remove the device hood to carry out certain maintenan-
ce and repair work.
Disassembly of the device hood, suitable for certain maintenance
and repair work inside a device.
Electric shock
Danger of electric shock from voltage-carrying parts inside the
device. The housing serves as a protective cover against voltages
inside the device.
ÎSwitch the device o before opening the device.
ÎPull the power plug.
5.1 Removing the device hood
The device hood is secured with T10 Torx screws:
6 Torx screws on the back of the device
The following steps are recommended for opening the housing:
Switch o the device and pull the power plug.
Remove the Torx screws of the device hood on the rear of the device.
Tilt the housing hood up and pull it towards you in order to remove
the hood.
T10 Torx screwdriver
Process Figure
Removing the
device hood
1. Loosen the 6 Torx screws
with an screwdriver and put
them aside.
Fig. 1: Remove Torx screws of
the device hood
Procedure
Tools

BlueShadow® Detector 50D Service Instructions, VSM-021
Disassembling the housing 9
Process Figure
1. Tilt the end of the device hood
1up and pull it towards you
with both hands.
Fig. 2: Removing the device
hood
The device has been opened.
Repair work possible:
Replacing the timing belt
Replacing the display
Replacing the lamps
Replacing the fan
Replacing the mainboard
Repairing the optical bench
Replacing the D2 power supply
5.2 Overview of the device
Legend:
1
Power supply for the
deuterium lamp
2
Device power supply
3
Fan
4
Mainboard
5
Deuterium lamp
housing
6
Motor
7
Timing belt
8
Filter unit
Fig. 3: Device with removed cover (Model E6520XC)
1
Result
Next steps
12
3
4
5
6
87

10
BlueShadow® Detector 50D Service Instructions, VSM-021
6. Replacing the display
The hood has been removed.
T10 Torx screwdriver
Note: A firmware update is possible via KNAUER Service tool.
Process Figure
Remove FFC from
mainboard
1. Remove the 5 pin flexible
flat cable (FFC) 1from the
mainboard.
Fig. 4: Remove FFC of the
mainboard
Removing lamp power
supply
2. Remove the T10 Torx screw 2.
3. Remove the D2 power supply
by pulling it up 3.
Fig. 5: Removing screw and
lamp power supply
Prerequisite
Tools
1
3
2

BlueShadow® Detector 50D Service Instructions, VSM-021
Replacing the display 11
Process Figure
Open fixing bracket of
keypad and removing
the display board
1. Open slide lock of keypad FFC
socket and pull out the FFC 4.
2. Remove 4 T10 Torx screws
5and 6and gently pull out
the display communication
board from display PCB.
3. Remove the display board 7.
Fig. 6: Removing display com-
munication mainboard
Fig. 7: Removing the display
The display communication board has been removed.
Remove the membrane keypad.
4
5
6
67 5
Result
Next step

12
BlueShadow® Detector 50D Service Instructions, VSM-021
7. Replacing the keypad
The keypad is adhesive. The adhesive surface is covered by a protective
film.
The device hood has been removed.
The following steps are recommended for replacing the keypad:
1. Remove cable.
2. Remove keypad.
3. Install new keypad.
Slotted screwdriver
Cloth and isopropanol or ethanol
7.1 Removing the keypad
Process Figure
1. Remove the keypad ribbon
cable 1from the display board
2.
Fig. 8: Keypad cable
connection
2. Remove the keypad 3by
sliding the slotted screwdriver
underneath one corner.
3. Lift the keypad up and pull it
from the device.
Fig. 9: Keypad
4. Carefully remove residues of
glue from the front plate. Use
a cloth and isopropanol or
ethanol.
Fig. 10: Cleaning the front plate
The keypad has been removed.
Install a new keypad.
Prerequisite
Procedure
Tools
12
3
Result
Next step

BlueShadow® Detector 50D Service Instructions, VSM-021
13
7.2 Installing a new keypad
Process Figure
1. Bend the ribbon cable 1away
from the keypad.
2. Remove the protective film 2
from the adhesive surface.
Pay attention that the ribbon
cable does not touch the
adhesive surface.
Note: Do not bend the keypad in
order not to damage the display
or the buttons. Fig. 11: Keypad
3. Guide the ribbon cable
through the slot 3in the front
plate.
4. Stick the keyboard to the front
panel.
Note: Do not press on the display
and the menu buttons for these
are very sensitive parts of the
keypad. Only press on the edge
of the keypad.
Fig. 12: Slot for keypad cable
5. Connect the ribbon cable 1to
the display board.
Fig. 13: Keypad cable connector
The keypad has been replaced.
2
1
3
Result

14
BlueShadow® Detector 50D Service Instructions, VSM-021
8. Replacing the lamps
To allow access to the device hood, make sure that the sides of the device
are not obstructed when replacing the lamps.
Electric shock
Danger of electric shock from voltage-carrying parts inside the device.
The housing serves as a protective cover against voltages inside the
device.
ÎSwitch the device o before opening the device.
ÎPull the power plug.
The device hood has been removed by loosening the T10 Torx screws.
Allen wrench for hexagonal screws (Allen screws), 2.5 mm diameter.
Legend:
1
Halogen lamp
2
Deuterium lamp
Fig. 14: The device hood has been removed
The detector 50D UV-VIS contains a deuterium and a halogen lamp, the
detector 50D UV only contains a deuterium lamp. It is recomended to
replace the halogen lamp after 1 000 operating hours and the D2 lamp
after 2 000 operating hours. The replacement unit consists of a lamp,
lamp socket, cable and plug. These components are permanently con-
nected together and are replaced as a unit. The halogen lamp uses a
dierent type of plug than the deuterium lamp in order to prevent acci-
dental mix-up.
Burns
Burn hazard from hot lamp. The lamp needs to cool down before
removal or cleaning.
ÎSwitch o the lamp meanwhile the device remains switched on for
further 15minutes, otherwise the lamp can not cool down.
ÎAfterward cool down, switch o the device and pull the power plug.
Prerequisite
Tools
2
1

15
BlueShadow® Detector 50D Service Instructions, VSM-021
9. Replacing the fan
Note the following when repairing the fan:
The fan is fastened on the rear panel of the device witha fan sleeve.
The fan can be removed from the inside of the device without the use
of tools.
To install the new fan, insert it into the sleeve and turn it until it snaps in
place.
Always order a complete set offan sleeve and fan.
Electric shock
Danger of electric shock from voltage-carrying parts inside the
device. The housing serves as a protective cover against voltages
inside the device.
ÎSwitch the device o before opening the device.
ÎPull the power plug.
9.1 Removing the fan
The device hood has been removed.
Process Figure
1. Remove the fan cable
from the PIN connector
of the mainboard.
Fig. 15: Fan cable at the mainboard
Prerequisite
Pulling out the fan

BlueShadow® Detector 50D Service Instructions, VSM-021
Replacing the fan 16
Process Figure
2. Turn the fan sleeve
clockwise until the
knobs align with the
bigger holes.
3. Pull the fan sleeve
together with the fan
out of the rear panel of
the device. This is pos-
sible without additional
tools.
4. Pull the fan out of the
fan sleeve and replace
it. Fig. 16: Fan knobs
Insert the new fan into the rear panel of the device.
9.2 Inserting a new fan
Note: Pay attention to the direction of the arrows printed on the fan.
Onearrow indicates the direction of rotation of the fan, theother the
direction in which the air is exhausted.
Procedure for removing the fan:
1. When inserting a new fan, pay attention to the arrow onthefan indi-
cating the direction of air flow.
2. Insert the fan cable into the nearest PIN connector.
Process Figure
1. Insert the new fan into
the fan sleeve.
2. Now push the fan slee-
ve through the wholes
in the rear panel of the
device.
3. Turn the fan sleeve
anticlockwise until the
knobs are back in their
former position.
Fig. 17: Fan knobs
Next step
Procedure
Inserting new fan