Knauer BlueShadow 50D User manual

Detector 50D
User Manual
V7654
BlueShadow
HPLC

50D user manual V7654, version 2.0
Table of Contents 3
Table of Contents
Note: For your own safety, read the manual and always observe the warn-
ings and safety information on the device and in the 
manual!
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Device Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Unpacking and Setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Contacting the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Power Supply and Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Power Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Power Plug . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Rear View of the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Controlling with the Multi-Pin Connector . . . . . . . . . . . . . . . . . . . . . . . . . 13
Pin Header Assignments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Analog Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Integrator Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Initial Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Checklist before initial operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Inserting the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Changing the optical path length of the preparative flow cell . . . . . . . . . . 16
Connecting the Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . . 19
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . 19
Operating the Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Control of the Detector with Chromatography Software . . . . . . . . . . . . . . . . 19
Switching the Detector on . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Handling the Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Overview of Touchscreen Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Graphical User Interface Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Status Display Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Main Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

50D user manual V7654, version 2.0
4TableofContents
Program Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Creating a Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Creating Program Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Deleting Program Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Running a Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Changing a Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Displaying the Program Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Delete a Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Creating a Program with a Starting Time . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Link Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Creating a Link . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Executing a Link . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Deleting a Link . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Scan Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Setup Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Setup Menu Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
GLP Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Standby . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Functionality Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Possible Problems and Rectifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Possible LAN Connection Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Maintenance and Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Contact to the Technical Support department . . . . . . . . . . . . . . . . . . . . . . . . 39
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Cleaning the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Rinsing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Cleaning the Lenses of an Analytical Flow Cell . . . . . . . . . . . . . . . . . . . . . . 41
Cleaning the Fiber Optics of a Preparative Flow Cell . . . . . . . . . . . . . . . . . 42
Replacing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Legal Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Abbreviations and Terminology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

50D user manual V7654, version 2.0
Table of Contents 5
To whom it may concern In case you prefer a French language user manual for this product, submit
your request including the corresponding serial number via email or fax to
KNAUER:
[email protected]
[phone]
Thank you.
A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit contacter
KNAUER par email ou par fax avec le no. de série:
[email protected]
[phone]
Merci beaucoup.

50D user manual V7654, version 2.0
6 Intended Use
Intended Use
Device Overview
The touchscreen, the LED light for standby mode, and the flow cell can be
found on the detector's front side.
The device can be operated from either the chromatography software at the
workstation, or the touchscreen.
The rear of the device contains the mains power connection, power switch,
connections for external devices, technical symbols and serial number,
among other things.
Remote
Control
Normally, the detector is controlled by the chromatography software
through a local network (LAN).
Automatic configuration The detector connected to the local area network (LAN) is automatically
detected by the chromatography software.
Device status When used in a local area network (LAN), the system status of the detector
can be checked using the chromatography software.
LAN setting The detector is set to DHCP (Dynamic Host Configuration Protocol) at the
factory. This means that the detector is automatically assigned an IP address
within the local network. This setting can be changed manually in the Setup
menu.
Legend
1Touchscreen
2LED light for standby
mode
3Flow cell with holder
Fig. 1 Front view of the device
123
Legend
1Serial number
2Integrator outlets
3LAN connection
4RS-232 port
5Fan opening
6Multi-pin connector
7Power switch
8Mains power connec-
tion
Fig. 2 Rear view of the device
1
8
23 45
7
6

50D user manual V7654, version 2.0
Intended Use 7
Laboratory Use Chemical analyses
Biochemical analyses
Chiral analyses
Food analyses
Pharmaceutical analyses
Environmental analyses
Features
The detector is a measuring system for fast and precise measurement in the
UV range and, with a halogen lamp (optional), also in the visible range. The
measurement principle is based on the attenuation of a monochromatic
light beam passing through liquid.
Self-calibration The self-calibration of the detector guarantees operation without external
settings. Type and combination of the installed lamps as well as the trans-
mission of the corresponding installed flow cell are analyzed and used for
the automatic setting of the integration time, in order to achieve a high
level of sensitivity.
Features Signal linearity and wavelength accuracy are verified by independent
measurements as part of a self-calibration.
The use of light sources for a specific spectral range is possible (UV: deu-
terium lamp, Vis: halogen lamp). A tandem operation with both light
sources is also possible, allowing a wavelength range of 190–900 nm to
be covered without changing the lamp.
Within a system, it is possible to measure four wavelengths simultane-
ously with the help of the software control.
Recording of a spectrum of 100 nm width in less than 100 ms.
The beam guidance is designed so that no chromatic aberration occurs
and thus the best possible useful signal is available, independent of the
selected wavelength.
Automatic recording and storage of the device-specific characteristics
that are important for GLP (Good Laboratory Practice) and OQ (Opera-
tion Qualification) or for the device service and device history. These are
operating times, operating parameters, wavelength accuracy and refer-
ence spectra.
Self-running and up-to-date device status diagnostics.
Control with HPLC software possible.
Easy integration of the detector into complex chromatography systems.
Extremely low noise level and baseline drift.
High data rates for fast chromatography.
Flexible options for use in the entire field of LC applications due to a
comprehensive range of flow cells for the detectors, from nano HPLC
cells with flow rates 100 nl/min to preparative flow cells with 10 l/min.

50D user manual V7654, version 2.0
8 Scope of Delivery
Eluents
Even small quantities of other substances, such as additives, modifiers, or
salts can influence the durability of the materials.
Note: The list of selected solvents was compiled based on research in the
pertinent literature and is only a recommendation. If there is any doubt,
contact the Technical Support of the manufacturer.
Scope of Delivery
Note: Only use original parts and accessories made by KNAUER or a com-
pany authorized by KNAUER
Suitable eluents Less suitable eluents Not suitable eluents
Acetone at 4°-25° C
(39.2°-77.0° F)1
Acetonitrile
Benzene
Chloroform
Ethyl acetate
Ethanol
Hexane/heptane at
4°–25° C (39.2°–
77.0° F)1
Isopropanol
Carbon dioxide
(liquid 99.999%
CO2)
Methanol
Phosphate buffer
solutions (0.5 M)
Toluol
Dilute ammonia
solution
Dilute acetic acid
(10-50%), at 25° C/
77.0° F
Dilute sodium
hydroxide (1M)
Water
1. valid for the specified temperature range
Dimethyl sulfoxide
(DMSO)
Slightly volatile elu-
ents
Methylene chloride
Tetrahydrofuran
(THF)
Dilute phosphoric
acid
Halogenated
hydrocarbons, e.g.
Freon®
Concentrated min-
eral and organic
acids
Concentrated bases
Eluents containing
particles
Perfluorinated elu-
ents, e. g. Fluori-
nert®FC-75, FC-40
Perfluorinated poly-
ether, e.g.
Fomblin®
Delivery Detector with test cell
User manual (EN/DE)
Accessory Kit
Transport protection

50D user manual V7654, version 2.0
Safety 9
Safety
Professional Group The user manual addresses persons who are qualified as chemical laboratory
technicians or have completed comparable vocational training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a limited
extent for use in liquid chromatography
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by the com-
pany KNAUER or an authorized company.
If you do not belong to this or a comparable professional group, you may
not perform the work described in this user manual under any circum-
stances. In this case, please contact your superior.
Safety Equipment When working with the device, take measures according to lab regulations
and wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
What must be taken into
account?
All safety instructions in the user manual
The environmental, installation, and connection specifications in the user
manual
National and international regulations pertaining to laboratory work
Original spare parts, tools, and solvents made or recommended by
KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident insurance
companies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once they have been used, never re-use capillaries in other areas of the
HPLC system.
Only use a given PEEK fitting for one specific port and never re-use it for
other ports. Always install new PEEK fittings on each separate port.
Follow KNAUER or manufacturer's instructions on caring for the columns
More safety-relevant information is listed below:
flammability: Organic solvents are highly flammable. Since capillaries can
detach from their screw fittings and allow solvent to escape, it is prohib-
ited to have any open flames near the analytical system.
solvent tray: Risk of electrical shock or short circuit if liquids get into the
device's interior. For this reason, place all bottles in a solvent tray.
solvent lines: Install capillaries and tubing in such a way that liquids can-
not get into the interior in case of a leak.
leaks: Regularly check if any system components are leaking.
power cable: Defective power cables are not to be used to connect the
device and the power supply system.
self-ignition point: Only use eluents that have a self-ignition point higher
than 150 °C under normal ambient conditions.
power strip: If several devices are connected to one power strip, always
consider the maximum power consumption of each device.
power supply: Only connect devices to voltage sources, whose voltage
equals the device's voltage.

50D user manual V7654, version 2.0
10 Safety
toxicity: Organic eluents are toxic above a certain concentration. Ensure
that work areas are always well-ventilated! Wear protective gloves and
safety glasses when working on the device!
Where is use of the device
prohibited?
Never use the system in potentially explosive atmospheres without appro-
priate protective equipment. For further information, contact the Technical
Support of KNAUER.
Decommissioning the
Device Securely
At any time, take the device completely out of operation by either switching
off the power switch or by pulling the power plug.
Opening the Device The device may be opened by the KNAUER Technical Support or any com-
pany authorized by KNAUER only.
Signal Words
Possible dangers related to the device are divided into personal and material
damage in this user manual.
Lethal injuries will occur.
Serious or moderate injuries can occur.
Minor injuries can occur.
Device defects can occur.
Decontamination
Contamination of devices with toxic, infectious or radioactive substances
poses a hazard for all persons during operation, repair, sale, and disposal of
a device.
Life-threatening injuries
Health danger if getting in contact with toxic, infectious or radio-active sub-
stances.
Before disposing of the device or sending it away for repair, you are
required to decontaminate the device in a technically correct manner.
All contaminated devices must be properly decontaminated by a specialist
company or the operating company before they can be recommissioned,
repaired, sold, or disposed of. All materials or fluids used for decontamina-
tion must be collected separately and disposed of properly.

50D user manual V7654, version 2.0
Symbols and Signs 11
Symbols and Signs
The following table explains symbols and labels which are used on the
device, in the chromatography software or in the user manual:
Unpacking and Setup
Contacting the Technical Support
You have various options to contact the Technical Support:
Phone [phone]
Fax [phone]
E-mail [email protected]
You can make your requests in English and German.
Location Requirements
Requirements The location for the device must meet the following requirements:
Device defect
The device overheats at exposure to sunlight and insufficient air circulation.
Device failures are very likely.
Set up the device in such a way that it is protected against exposure to
direct sunlight.
Keep at least 15 cm clear at the rear and 5–10 cm at each side for air cir-
culation.
Symbol Meaning
Electric shock hazard
Electrostatic discharge hazard, damages to system,
device, or components can occur.
A device or system marked with CE fulfills the product
specific requirements of European directives. This is
confirmed in a Declaration of Conformity.
Testing seals in Canada and the USA at nationally recog-
nized testing centers (NRTL). The certified device or sys-
tem has successfully passed the quality and security
tests.
level surface for device or system
Protect from heavy ventilation
Weight 5.3 kg
Dimensions 242 x 169 x 399 mm (W x H x D)
Power Supply 100 – 240 V; 50 – 60 Hz; 75 W
Air humidity below 90 %, non condensing
Temperature range 4 – 40 °C; 39.2 – 104 °F