Knauer Axura DAD 6.1L User manual

Detector DAD 6.1L, DAD 2.1L, MWD 2.1L
User Manual
V6700

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Table of Contents 3
Table of Contents
Note For your own safety, read the manual and always observe the warnings and
safety information on the device and in the manual!
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Device Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Rear View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Side View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Suitable Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Less Suitable Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Unsuitable Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Safety for Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Contact Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Operating Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Inserting the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Inserting the Flow Cell with Fiber Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Connecting the Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Using the PEEK Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Connecting the Leak Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Connecting the Device to the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Setting a Static IP Address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Using Remote Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Connections to the Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Connecting Cables to the Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Integrator Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Integrating the Detector into the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Pre-Installed Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
4TableofContents
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Initial Start Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Switch On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Operating with Chromatography Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Operating with Control Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Operating with Mobile Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Meaning of the LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Default Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Optimizing the Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Warm Up Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Selecting the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Flow Cell Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Path Length . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Wetted Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Pressure Stability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Remote Operation (Fiber Optics) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Selecting the Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Bandwidth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Spectral Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Time Constant & Data Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Integration Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Subtraction of the Baseline Chromatogram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Extended Linear Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Functionality Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Possible Problems and Rectifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Maintenance and Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Contacting the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Maintenance Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Checking the Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Decommissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Disconnecting the Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Cleaning the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Basic Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Advanced Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Table of Contents 5
Replacing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Replacing the Lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Removing the Deuterium or Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Installing the Deuterium or Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Removing the Leak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
DAD 6.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
DAD 2.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
MWD 2.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Conditions for Specification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Legal Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Warranty Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Repeat Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Device and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Flow Cells with Fiber Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Fiber Optic Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
HPLC Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
To whom it may concern In case you prefer a French language user manual for this product, submit
your request including the corresponding serial number via email or fax to
KNAUER:
[email protected]
[phone]
Thank you.
A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit contacter
KNAUER par email ou par fax avec le no. de série:
[email protected]
[phone]
Merci beaucoup.

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
6 Intended Use
Intended Use
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the device
could fail.
Description The detector can be used for analytical, semi-preparative and preparative
applications.
Operating Ranges The device can be used in the following areas:
biochemical analysis
chemical analysis
food analysis
pharmaceutical analysis
environmental analysis
Device Overview
Front View
On the front of the device, there are the flow cell and the removable lamp
cover for lamp replacement.
Rear View
On the back of the detector, the connectors for LAN and power plug are
located, as well as the serial number of the device and the power switch
(on/off).
RS-232, Service Interface Please note that the service interface is solely used for repair and mainte-
nance tasks performed by service technicians.
Legend
1leak tray
2capillary guide
3lamp cover
4serial number
5leak management
6switch/standby key
7status LED
8flow cell
Fig. 1 front view (example DAD 6.1L)
8
7
2
3
2
2
4
1
6
5
Legend
1device information
2integrator outlets
3LAN connector
4service interface
5multi-pin connector
6fan
7connection and
power switch
Fig. 2 rear view (example DAD 6.1L)
2345 671

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Intended Use 7
Side View
On the side of the detector, you find the connector for the operating panel
Control Unit in chapter ’Operating with Control Unit’ on page 26.
Features
In an HPLC system, the detector serves to detect substances in liquids and
show their concentration. The sensitivity of the detector depends on the
installed flow cell.
Optical Module The detectors measure the light absorption of the sample in the ultraviolet
and visual spectra. The entire spectrum permeates the sample and after-
wards is split up by a grate. The split light falls onto a geometric array of
1024 (DAD 6.1L) or 256 (DAD 2.1L, MWD 2.1L) separate photodiodes –
the photodiode array.
The optical system of the DAD 6.1L is depicted in figure 4. An active tem-
perature control with 0.5 °C sensitivity ensures baseline stability for minimal
baseline drift.
Lamps The lamps can be exchanged quickly and easily without the need of a ser-
vice technician.
DAD 6.1L Two light sources, a deuterium lamp and a halogen lamp, cover the entire
UV-Vis wavelength range.
DAD 2.1L, MWD 2.1L One deuterium lamp covers the UV wavelength range.
Mirror
DAD 6.1L
Maximum light intensity is provided by effectively mixing the light sources
from the deuterium and halogen lamp with a mirror using Polka Dot tech-
nology.
Legend
1USB port for the
Control Unit
Fig. 3 USB port for the Control Unit
1
Legend
1deuterium lamp
2halogen lamp
3flow cell
4diode array sensor
5diffraction grating
6mirror
Fig. 4 optical system DAD 6.1L
3
5
1
2
4
6

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
8 Intended Use
Flow Cell Various types of flow cells are available to the user and allow a variety of
applications. Flow cell cartridges with KNAUER PressureProof and KNAUER
LightGuide technology are available (see “Flow Cells” on page 52).
KNAUER LightGuide
Flow Cell Cartridges
KNAUER LightGuide flow cell cartridges combine a maximum light trans-
mission (using total reflection) with a minimum cell volume to offer an ideal
signal-to-noise ration.
KNAUER PressureProof
Flow Cell Cartridges
The KNAUER PressureProof flow cell cartridges are optimized for conven-
tional and high flow HPLC and FPLC applications. These flow cells feature an
increased pressure stability (up to 300 bar) and extended flow rate range
(up to 20 and 50 ml/min).
KNAUER Fiber Optics
Technology
Adapters for fiber optics are optionally available. Fiber optics allow separat-
ing the flow cell from the detector. This way, working with dangerous,
explosive, or toxic materials becomes safer. Remote flow cells are recom-
mended for preparative applications (high flow rates), in order to protect
sensitive optical components from potential leakages.
Data Rate The detector measures with a maximum data rate of 100 Hz. During the
measurement, approx. 100 spectra per second are recorded.
Leak Management Integrated leak management drains liquids from leaks.
GLP Data The Mobile Control, the Control Unit and the different software products
(ClarityChrom®or OpenLAB®) can be used to display or read GLP data like
operating hours, lamp operating hours, number of lamp ignitions, etc. You
find a detailed description on how to display or read out GLP data in the
respective user manuals.
Upgrade MWD 2.1L The multiwavelength detector can be upgraded to a diode array detector.
Optional Accessories The following optional accessories are available:
Mobile Control
Control Unit
Eluents
Even small quantities of other substances, such as additives, modifiers, or
salts can influence the durability of the materials.
Note: The list of selected solvents was compiled based on research in the
pertinent literature and is only a recommendation by KNAUER. If there is
any doubt, contact the Technical Support of the manufacturer.
Suitable Eluents
acetate buffer solutions
acetone at 4–25 °C (39.2–77.0 °F)1
acetonitrile2
benzene
chloroform
ethyl acetate
ethanol
formiate buffer solution
isopropanol
carbon dioxide (liquid 99.999 % CO2)
methanol
phosphate buffer solutions (0.5 M)
toluol
ammoniated dilute solution
acetic acid (0.1–1 %), at 25 °C
1. valid for the specified temperature range
2. not recommended in combination with PEEK small parts and PEEK capillaries

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Scope of Delivery 9
sodium hydroxide (1 M)
water
Less Suitable Eluents
diethylamine (0.1 %) (DEA)
dimethyl sulfoxide (DMSO)
slightly volatile eluents
methylene chloride1
tetrahydrofuran (THF)1
phosphoric dilute acid
triethylamine (0.1 %) (TEA)
trifluoroacetic acid (0.1 %) (TFA)
Unsuitable Eluents
halogenated hydrocarbons, e.g. Freon®
concentrated mineral and organic acids
concentrated bases
eluents containing particles
perfluorinated eluents, e.g. Fluorinert® FC-75, FC-40
perfluorinated polyether, e.g. Fomblin®
Scope of Delivery
Note: Only use original parts and accessories made by the manufacturer or
a company authorized by the manufacturer.
1. not recommended in combination with PEEK small parts and PEEK capillaries
DAD 6.1L detector with test cell
power cable
user manual German/English
Installation Qualification
Accessories Kit DAD 6.1L, DAD 2.1L, MWD 2.1L
AZURA
DAD 2.1L detector with test cell
power cable
user manual German/English
Installation Qualification
Accessories Kit DAD 6.1L, DAD 2.1L, MWD 2.1L
AZURA
MWD 2.1L detector with test cell
power cable
user manual German/English
Installation Qualification

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
10 Safety for Users
Safety for Users
Professional Group The user manual addresses persons who are qualified as chemical laboratory
technicians or have completed comparable vocational training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a limited
extent for use in liquid chromatography
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by the com-
pany KNAUER or an authorized company.
If you do not belong to this or a comparable professional group, you may
not perform the work described in this user manual under any circum-
stances. In this case, please contact your superior.
Safety Equipment When working with the device, take measures according to lab regulations
and wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
What must be taken into
account?
All safety instructions in the user manual
The environmental, installation, and connection specifications in the user
manual
National and international regulations pertaining to laboratory work
Original spare parts, tools, and solvents made or recommended by
KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident insurance
companies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once they have been used, never re-use capillaries in other areas of the
HPLC system.
Only use a given PEEK fitting for one specific port and never re-use it for
other ports. Always install new PEEK fittings on each separate port.
Follow KNAUER or manufacturer's instructions on caring for the columns
More safety-relevant information is listed below:
flammability: Organic solvents are highly flammable. Since capillaries can
detach from their screw fittings and allow solvent to escape, it is prohib-
ited to have any open flames near the analytical system.
solvent tray: Risk of electrical shock or short circuit if liquids get into the
device's interior. For this reason, place all bottles in a solvent tray.
solvent lines: Install capillaries and tubing in such a way that liquids can-
not get into the interior in case of a leak.
leaks: Regularly check if any system components are leaking.
power cable: Defective power cables are not to be used to connect the
device and the power supply system.
self-ignition point: Only use eluents that have a self-ignition point higher
than 150 °C under normal ambient conditions.
Accessories Kit DAD 6.1L, DAD 2.1L, MWD 2.1L
AZURA

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Safety for Users 11
power strip: If several devices are connected to one power strip, always
consider the maximum power consumption of each device.
power supply: Only connect devices to voltage sources, whose voltage
equals the device's voltage.
toxicity: Organic eluents are toxic above a certain concentration. Ensure
that work areas are always well-ventilated! Wear protective gloves and
safety glasses when working on the device!
Where is use of the device
prohibited?
Never use the system in potentially explosive atmospheres without appro-
priate protective equipment. For further information, contact the Technical
Support of KNAUER.
Secure decommissioning At any time, take the device completely out of operation by either switching
off the power switch or by pulling the power plug.
Opening the Device The device may be opened by the KNAUER Technical Support or any com-
pany authorized by KNAUER only.
Signal Words
Possible dangers related to the device are divided into personal and material
damage in this user manual.
Lethal injuries will occur.
Serious or moderate injuries can occur.
Minor injuries can occur.
Device defects can occur.
Decontamination
Contamination of devices with toxic, infectious or radioactive substances
poses a hazard for all persons during operation, repair, sale, and disposal of
a device.
Life-threatening injuries
Health danger if getting in contact with toxic, infectious or radio-active sub-
stances.
Before disposing of the device or sending it away for repair, you are
required to decontaminate the device in a technically correct manner.
All contaminated devices must be properly decontaminated by a specialist
company or the operating company before they can be recommissioned,
repaired, sold, or disposed of. All materials or fluids used for decontamina-
tion must be collected separately and disposed of properly.
Decontamination Report Devices without a completed Decontamination Report will not be repaired.
If you would like to return a device to KNAUER, make sure to enclose a com-
pleted Decontamination Report with the device: http://www.knauer.net/
en/downloads/service.html

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
12 Symbols and Signs
Symbols and Signs
The following symbols and signs can be found on the device:
Installation
This chapter describes all preparatory steps prior to the start-up. If you
encounter difficulties during installation, contact the Technical Support.
Contact Data
Transport
Carefully prepare the device for transport or storage. If you want to return
your device to KNAUER for repairs, enclose the Service Request Form which
can be downloaded from our website.
Device Data For a secure transport, note the weight and dimensions of the device (see
Technical Data).
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
Lift the device on the side of the housing only.
Lifiting Clasp the device at its side panels and lift it out of the packaging. Do not
hold onto front cover or leak tray.
Symbol Meaning
Electric shock hazard
Electrostatic discharge hazard, damages to sys-
tem, device, or components can occur.
Obey maximum load for leak tray during trans-
portation, installation and operation.
A device or system marked with CE fulfills the
product specific requirements of European direc-
tives. This is confirmed in a Declaration of Confor-
mity.
Testing seals in Canada and the USA at nationally
recognized testing centers (NRTL). The certified
device or system has successfully passed the qual-
ity and security tests.
Phone [phone]
Fax [phone]
E-mail [email protected]

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Installation 13
Operating Environment
Only if the requirements for ambient conditions of the operating environ-
ment are met, can the intended use be ensured. Details on the operating
conditions can be found in the Technical Data section.
Device defect
The device overheats at exposure to sunlight and insufficient air circulation.
Device failures are very likely.
Set up the device in such a way that it is protected against exposure to
direct sunlight.
Keep at least 15 cm clear at the rear and 5–10 cm at each side for air cir-
culation.
Space Requirements At least 5 cm if another device is set up on one side
At least 10 cm if further devices are set up on both sides
At least 15 cm on the rear panel for the fan.
Leave the power plug on the rear of the device accessible to be able to
disconnect the device from the mains.
General requirements Position the device on a level and even surface.
Protect the device against direct exposure to sunlight.
Set up the device at a location not exposed to air drafts (A/C systems).
Do not set up the device in the vicinity of other machines that cause floor
vibrations.
Avoid sources of high frequencies near the device. High-frequency
sources may compromise measuring values.
Earthquake Areas If you are located in an earthquake area, use the bore holes 1in the side
panels to secure the device. The bore holes are located on either right or left
side panel.
Setup
Prerequisite Check packaging for damage caused during transportation. If necessary, put
forward any claim for damages to the carrier.
Tools Utility knife
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
Lift the device on the side of the housing only.
Fig. 5 bore holes of device
1

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
14 Installation
Process 1. Set up the package in such a way that you can read the label. Using the
utility knife, cut the adhesive tape and open the packaging.
2. Remove the foam insert. Take out the accessory kit and the manual.
3. Open the accessory kit and check the scope of delivery. In case any parts
are missing, contact the Technical Support.
4. Clasp the device from below, lift it out of the packaging and place it on
its feet. Do not hold onto the front cover.
5. Check the device for signs of damage that occurred during transport. In
case you notice any damage, contact the Technical Support.
6. Place the device in its site of operation and remove protective foil.
Next steps Store packaging and keep the included packing list for repeat orders.
Note: The test cell is used during operation qualifications and must be
stored.
Inserting the Flow Cell
Before taking a flow cell filled with solvent into operation, please make cer-
tain that the used eluent is miscible with that one used previously. Other-
wise purge the flow cell with a medium miscible with both the eluents.
Prerequisites No flow cell or test cell is installed.
The device is switched off.
Note: Pay attention to the compatibility of the flow cells.
Practical Tip: Unscrew the capillary for easier handling.
Eye injury
Irritation of retina through UV light. Concentrated UV light can leak out
from the flow cell or the fiber optic connectors.
Switch off the device or the lamps.
Performance decrease
Any components in the light path like fiber optic ends become contami-
nated, if being touched with the fingers.
Avoid touching the ends without gloves.
Use alcohol and a cotton bud for cleaning.
Next Steps Connect the capillaries.
Inserting the Flow Cell with Fiber Optics
KNAUER fiber optic cables are extremely robust. Nevertheless fiber optic
cables are sensitive to bending. The minimum bending radius is 100 mm
(short term: 70 mm).
Observe the following regarding the use of fiber optics:
UV light will cause the fiber optic to become blind with time (solariza-
tion), making them no longer suitable for use.
Process Process Figure
Place the flow cell into the opening
and slide to the back until it clicks
into place inside the device.
Fig. 6 flow cell

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Installation 15
Do not touch the ends of the fiber optic with your fingers, as this could
falsify the measurement.
Handle the fiber optic with care, avoid impacts or hard actions.
Move the fiber optic carefully without using pressure or bending it.
The flow cells with fiber optics are connected via an adapter.
Prerequisites No flow cell or test cell is installed.
Auxiliary Material Screw-type cap fittings for the fiber optics
Eye injury
Irritation of retina through UV light. Concentrated UV light can leak out
from the flow cell or the fiber optic connectors.
Switch off the device or the lamps.
Note: Fiber optical connectors, like all other optical parts, should never be
touched without wearing gloves because otherwise contaminations may
reduce light intensity. Contaminated parts may be cleaned with a soft cloth
and isopropanol.
Next Steps Connect the capillaries.
Connecting the Capillaries
Capillaries connect the detector with the column and guide liquids. For the
flow cell connectors, we recommend PEEK capillaries and PEEK fittings.
Prerequisites The flow cell was inserted.
Screw-type cap fittings and cap fittings were removed.
Note: The KNAUER LightGuide flow cell cartridges have unisex input and
output connections.
Component defect
Damage to the ports caused by strongly tightened fittings.
Use 5 Nm torque for stainless steel fittings.
Use 0.5 Nm torque for PEEK fittings.
Process Process Figure
1. Remove the cap fittings 1from
the adapter.
2. Place the adapter into the open-
ing and slide to the back until it
clicks into place inside the
device.
Fig. 7 fiber optics adapter
3. Remove the screw-type cap fit-
tings 2from the fiber optics.
4. Manually, screw the fiber optics
to the adapter. Fig. 8 cap fitting on the fiber optics
5. Remove the cap fittings from
the flow cell.
6. Manually, screw the fiber optics
3to the flow cell.
Fig. 9 fitting of the fiber optics on
the flow cell
1
2
3

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
16 Installation
Next Steps Bring the device into operation.
Using the PEEK Fittings
PEEK fittings withstand a maximum pressure of 400 bar. Note the torque of
0.5 Nm.
Connecting the Leak Management
The leak management consists of the leak sensor and the drainage system
(funnels, hoses, nozzles). The drainage system ensures that escaping liquids
flow into a waste bottle. When leaks are registered by the leak sensor, the
LED flashes red. Both the device and the data acquisition via chromatogra-
phy software are stopped.
Prerequisite The front cover has been removed.
Process Process Figure
1. Push the capillary 1through
the fitting 2.
2. Slide the lock ring 3over the
tubing. Note that the tapered
end of the lock ring has to point
to the seal ring 4.
3. Attach the seal ring 4.
Fig. 10 capillary fittings
4. Fasten the capillary at the flow
cell 4fingertight.
Fig. 11 flow cell with capillary
1324
13
24
4
4
Process Process Figure
1. Carefully push the funnel 1into
the center opening of the capil-
lary guide 2.
Fig. 12 Funnel and capillary guide
2. Push the long ending of the first
nozzle 4into the hose 3.
Fig. 13 Hose and nozzle
12
3
4

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Installation 17
Next steps Attach the front cover.
Control
The detector can be operated in two ways:
via remote connector
as part of a LAN, via the LAN connector of the router
All connectors for external control are located on the rear side of the detec-
tor.
The detector can also be controlled via Control Unit or Mobile Control. The
Control Unit connector is located on the side of the device.
3. Connect the nozzle and the fun-
nel.
4. Push the other end of the hose
onto the nozzle 5of the leak
tray.
Fig. 14 Hose connected to device
5. For the bottom device, push the
short end of the nozzle 6 into
the opening in the collection
point of the leak tray.
6. Connect the hose to the nozzle
and lead the second ending to
the waste bottle.
7. Place the waste bottle below the
bottom device.
Fig. 15 Leak tray with nozzle
Process Process Figure
5
6
Legend
1integrator outlets
2LAN connector
3service interface
4multi-pin connector
Fig. 16 connectors on rear side
1423

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
18 Installation
Connecting the Device to the Computer
This section describes how to set up an HPLC system in a local area network
(LAN) and how a network administrator can integrate this LAN into your
company network. The description applies to the operating system Win-
dows and all conventional routers.
Note: To set up a LAN, we recommend to use a router. That means the fol-
lowing steps are required:
Process 1. On the computer, go to the control panel and check the LAN properties.
2. Hook up the router to the devices and the computer.
3. On the computer, configure the router to set up the network.
4. Install the chromatography software from the data storage device.
5. Switch on the device and run the chromatography software.
Configuring the LAN Settings
The LAN uses only one server (which is normally the router) from that the
devices automatically receive their IP address.
Prerequisite In Windows, power saving, hibernation, standby, and screen saver must
be deactived.
In case you use an USB-to-COM box, the option "Allow the computer to
turn off ths device to save power" in the devicemanager must be deacti-
vated for all USB hosts.
Only for Windows 7: For the network adapter, the option "Allow the
computer to turn off this device to save power" in the Device Manager
must be deactivated.
Process 1. In Windows 7 choose Start
 Control Panel  Network and Sharing Cen-
ter.
2. Double-click on LAN Connection.
3. Click on the button Properties.
4. Select Internet Protocol version 4 (TCP/IPv4).
5. Click on the button Properties.
6. Check the settings in the tab General. The correct settings for the DHCP
client are:
a) Obtain IP address automatically
b) Obtain DNS server address automatically
7. Click on the button OK.
Connecting the Cables
A router 3has several LAN ports 2and one WAN port 4that can be used
to integrate the LAN into a wide area network (WAN), e.g. a company net-
work or the Internet. In contrast, the LAN ports serve to set up a network
from devices 1and a computer 5. To avoid interference, we recommend
operating the HPLC system separately from the company network.
1USB port for the
Control Unit
Fig. 17 USB port for the Control Unit
5

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Installation 19
You will find patch cables for each device and the router in the accessories
kit. To connect the router to a WAN, an additional patch cable is required,
which is not supplied within the scope of delivery.
Prerequisite The computer has been switched off.
There is a patch cable for each device and the computer.
Process 1. Use the patch cable to connect the router and the computer. Repeat this
step to connect all devices.
2. Use the power supply to connect the router to the mains power system.
Configuring the Router
The router is preset at the factory. You will find a label at the bottom side of
the router, on which IP address, user name, and password are printed.
These information help to open the router configuration.
Process 1. To open the router configuration, start your Internet browser and enter
the IP address (not for all routers).
2. Enter user name and password.
3. Configure the router as DHCP server.
4. In the router configuration, check the IP address range and make
changes if necessary.
Result Once the router has assigned IP addresses to all devices, the chromatogra-
phy software can be used to remotely control the system.
Integrating the LAN into a Company Network
A network administrator can integrate the LAN into your company network.
In this case you use the WAN port of the router.
Prerequisite There is a patch cable for the connection.
Process 1. Check that the IP address range of the router and of the company net-
work do not overlap.
2. In case of an overlap, change the IP address range of the router.
3. Use the patch cable to connect the router WAN port to the company net-
work.
4. Restart all devices, including the computer.
Controlling Several Systems Separately in a LAN
Devices connected to a LAN communicate through ports, which are part of
the IP address. If more than one HPLC system is connected to the same LAN
and you plan on controlling them separately, you can use different ports to
avoid interference. Therefore, the port number for each device must be
changed and this same number must be entered into the device configura-
tion of the chromatography software. We recommend to use the same port
number for all devices in the same system.
Note: The port is set to 10001 at the factory. You must use the same num-
bers in the device configuration of the chromatography software as in the
device, otherwise the connection fails.
Process 1. Find out port number and change it on the device.
12
345

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
20 Installation
2. Enter the port number in the chromatography software.
Result The connection is established.
Setting a Static IP Address
A static IP address can be set via the Mobile Control.
Note: Please inform yourself about the IT safety standards valid for your lab-
oratory, before changing the LAN settings.
The device is factory set to a dynamic IP address (DHCP). To ensure a per-
manent LAN connection between the chromatography software and the
device, we recommend to set a static IP address for certain applications.
Prerequisites The device has been switched on.
Mobile Control has been installed and started.
The connection between the Mobile Control and the device has been
established.
Note: You find further information on LAN settings in the chapter Device
Settings of the Mobile Control User Manual.
Procedure 1. In the Mobile Control, choose Settings .
2. On the General tab, choose the device name.
3. Under Network Settings, choose the setting Static 1.
4. Enter the IP address into the text box IP Address 2.
5. If necessary, change the subnet mask and the gateway 3.
6. Click in the top right corner.
7. Restart the device.
Next steps In case necessary, go back to the original setting with the [Reset] button that
can be found under Settings > General > Network Settings > LAN Settings.
Using Remote Control
On the rear panel of the detectors an electrical connector socket is located
which serves to send or receive signals from other instruments. For example
start signals from an injection valve or an autosampler can be put to the
START input. All voltages have to be mounted between GROUND and the
corresponding event.
Electronic defect
Electrostatic discharge can destroy the electronics.
Wear a protective bracelet against electrostatic discharge and ground.
For test purposes or in some other cases, it can make sense to manually
enter these signals.
sending control signals (Events) to external devices
opening and closing contacts
activating 500 ms pulses
1
2
3

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Installation 21
The following remote signals can be received and sent:
for receiving start, control, and error signals from external devices
for sending start, control and error signals to external devices
Connections to the Terminal Strip
Fig. 18 relation between display and terminal strip
Connection Function
EV 1
(Event 1)
Relay Contact
The contact is on a floating basis. Its setting
depends on the settings in the Control Unit,
Mobile Control, or software.
Steady-rate signal:
passive = open relay contact
active = closed relay contact
Pulse:
Closed relay contact for at least 1000 ms
Permissible load of the relay contact: 1 A/ 24
V DC
EV 2 (Event 2) TTL Output
Levels:
passive 5 V
active 0 V
Pulse:
0 V for at least 1000 ms
Error OUT TTL Output
Levels:
passive 5 V
active 0 V
Output is active until the Error condition has
been eliminated.
Error IN TTL Input
Low active
Secure switching threshold at least 10 mA
After receiving a signal (short-circuit to ground)
from an external device, an error message appears
and the device stops.