Knauer Axura DAD 6.1L User manual

Detector DAD 6.1L, DAD 2.1L, MWD 2.1L
User Manual
V6700

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Table of Contents 3
Table of Contents
Note For your own safety, read the manual and always observe the warnings and
safety information on the device and in the manual!
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Device Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Rear View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Side View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Suitable Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Less Suitable Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Unsuitable Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Safety for Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Contact Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Operating Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Inserting the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Inserting the Flow Cell with Fiber Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Connecting the Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Using the PEEK Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Connecting the Leak Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Connecting the Device to the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Setting a Static IP Address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Using Remote Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Connections to the Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Connecting Cables to the Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Integrator Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Integrating the Detector into the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Pre-Installed Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
4TableofContents
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Initial Start Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Switch On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Operating with Chromatography Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Operating with Control Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Operating with Mobile Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Meaning of the LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Default Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Optimizing the Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Warm Up Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Selecting the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Flow Cell Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Path Length . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Wetted Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Pressure Stability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Remote Operation (Fiber Optics) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Selecting the Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Bandwidth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Spectral Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Time Constant & Data Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Integration Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Subtraction of the Baseline Chromatogram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Extended Linear Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Functionality Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Possible Problems and Rectifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Maintenance and Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Contacting the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Maintenance Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Checking the Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Decommissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Disconnecting the Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Cleaning the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Basic Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Advanced Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Table of Contents 5
Replacing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Replacing the Lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Removing the Deuterium or Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Installing the Deuterium or Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Removing the Leak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
DAD 6.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
DAD 2.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
MWD 2.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Conditions for Specification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Legal Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Warranty Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Repeat Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Device and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Flow Cells with Fiber Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Fiber Optic Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
HPLC Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
To whom it may concern In case you prefer a French language user manual for this product, submit
your request including the corresponding serial number via email or fax to
KNAUER:
[email protected]
[phone]
Thank you.
A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit contacter
KNAUER par email ou par fax avec le no. de série:
[email protected]
[phone]
Merci beaucoup.

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
6 Intended Use
Intended Use
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the device
could fail.
Description The detector can be used for analytical, semi-preparative and preparative
applications.
Operating Ranges The device can be used in the following areas:
biochemical analysis
chemical analysis
food analysis
pharmaceutical analysis
environmental analysis
Device Overview
Front View
On the front of the device, there are the flow cell and the removable lamp
cover for lamp replacement.
Rear View
On the back of the detector, the connectors for LAN and power plug are
located, as well as the serial number of the device and the power switch
(on/off).
RS-232, Service Interface Please note that the service interface is solely used for repair and mainte-
nance tasks performed by service technicians.
Legend
1leak tray
2capillary guide
3lamp cover
4serial number
5leak management
6switch/standby key
7status LED
8flow cell
Fig. 1 front view (example DAD 6.1L)
8
7
2
3
2
2
4
1
6
5
Legend
1device information
2integrator outlets
3LAN connector
4service interface
5multi-pin connector
6fan
7connection and
power switch
Fig. 2 rear view (example DAD 6.1L)
2345 671

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Intended Use 7
Side View
On the side of the detector, you find the connector for the operating panel
Control Unit in chapter ’Operating with Control Unit’ on page 26.
Features
In an HPLC system, the detector serves to detect substances in liquids and
show their concentration. The sensitivity of the detector depends on the
installed flow cell.
Optical Module The detectors measure the light absorption of the sample in the ultraviolet
and visual spectra. The entire spectrum permeates the sample and after-
wards is split up by a grate. The split light falls onto a geometric array of
1024 (DAD 6.1L) or 256 (DAD 2.1L, MWD 2.1L) separate photodiodes –
the photodiode array.
The optical system of the DAD 6.1L is depicted in figure 4. An active tem-
perature control with 0.5 °C sensitivity ensures baseline stability for minimal
baseline drift.
Lamps The lamps can be exchanged quickly and easily without the need of a ser-
vice technician.
DAD 6.1L Two light sources, a deuterium lamp and a halogen lamp, cover the entire
UV-Vis wavelength range.
DAD 2.1L, MWD 2.1L One deuterium lamp covers the UV wavelength range.
Mirror
DAD 6.1L
Maximum light intensity is provided by effectively mixing the light sources
from the deuterium and halogen lamp with a mirror using Polka Dot tech-
nology.
Legend
1USB port for the
Control Unit
Fig. 3 USB port for the Control Unit
1
Legend
1deuterium lamp
2halogen lamp
3flow cell
4diode array sensor
5diffraction grating
6mirror
Fig. 4 optical system DAD 6.1L
3
5
1
2
4
6

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
8 Intended Use
Flow Cell Various types of flow cells are available to the user and allow a variety of
applications. Flow cell cartridges with KNAUER PressureProof and KNAUER
LightGuide technology are available (see “Flow Cells” on page 52).
KNAUER LightGuide
Flow Cell Cartridges
KNAUER LightGuide flow cell cartridges combine a maximum light trans-
mission (using total reflection) with a minimum cell volume to offer an ideal
signal-to-noise ration.
KNAUER PressureProof
Flow Cell Cartridges
The KNAUER PressureProof flow cell cartridges are optimized for conven-
tional and high flow HPLC and FPLC applications. These flow cells feature an
increased pressure stability (up to 300 bar) and extended flow rate range
(up to 20 and 50 ml/min).
KNAUER Fiber Optics
Technology
Adapters for fiber optics are optionally available. Fiber optics allow separat-
ing the flow cell from the detector. This way, working with dangerous,
explosive, or toxic materials becomes safer. Remote flow cells are recom-
mended for preparative applications (high flow rates), in order to protect
sensitive optical components from potential leakages.
Data Rate The detector measures with a maximum data rate of 100 Hz. During the
measurement, approx. 100 spectra per second are recorded.
Leak Management Integrated leak management drains liquids from leaks.
GLP Data The Mobile Control, the Control Unit and the different software products
(ClarityChrom®or OpenLAB®) can be used to display or read GLP data like
operating hours, lamp operating hours, number of lamp ignitions, etc. You
find a detailed description on how to display or read out GLP data in the
respective user manuals.
Upgrade MWD 2.1L The multiwavelength detector can be upgraded to a diode array detector.
Optional Accessories The following optional accessories are available:
Mobile Control
Control Unit
Eluents
Even small quantities of other substances, such as additives, modifiers, or
salts can influence the durability of the materials.
Note: The list of selected solvents was compiled based on research in the
pertinent literature and is only a recommendation by KNAUER. If there is
any doubt, contact the Technical Support of the manufacturer.
Suitable Eluents
acetate buffer solutions
acetone at 4–25 °C (39.2–77.0 °F)1
acetonitrile2
benzene
chloroform
ethyl acetate
ethanol
formiate buffer solution
isopropanol
carbon dioxide (liquid 99.999 % CO2)
methanol
phosphate buffer solutions (0.5 M)
toluol
ammoniated dilute solution
acetic acid (0.1–1 %), at 25 °C
1. valid for the specified temperature range
2. not recommended in combination with PEEK small parts and PEEK capillaries

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Scope of Delivery 9
sodium hydroxide (1 M)
water
Less Suitable Eluents
diethylamine (0.1 %) (DEA)
dimethyl sulfoxide (DMSO)
slightly volatile eluents
methylene chloride1
tetrahydrofuran (THF)1
phosphoric dilute acid
triethylamine (0.1 %) (TEA)
trifluoroacetic acid (0.1 %) (TFA)
Unsuitable Eluents
halogenated hydrocarbons, e.g. Freon®
concentrated mineral and organic acids
concentrated bases
eluents containing particles
perfluorinated eluents, e.g. Fluorinert® FC-75, FC-40
perfluorinated polyether, e.g. Fomblin®
Scope of Delivery
Note: Only use original parts and accessories made by the manufacturer or
a company authorized by the manufacturer.
1. not recommended in combination with PEEK small parts and PEEK capillaries
DAD 6.1L detector with test cell
power cable
user manual German/English
Installation Qualification
Accessories Kit DAD 6.1L, DAD 2.1L, MWD 2.1L
AZURA
DAD 2.1L detector with test cell
power cable
user manual German/English
Installation Qualification
Accessories Kit DAD 6.1L, DAD 2.1L, MWD 2.1L
AZURA
MWD 2.1L detector with test cell
power cable
user manual German/English
Installation Qualification

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
10 Safety for Users
Safety for Users
Professional Group The user manual addresses persons who are qualified as chemical laboratory
technicians or have completed comparable vocational training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a limited
extent for use in liquid chromatography
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by the com-
pany KNAUER or an authorized company.
If you do not belong to this or a comparable professional group, you may
not perform the work described in this user manual under any circum-
stances. In this case, please contact your superior.
Safety Equipment When working with the device, take measures according to lab regulations
and wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
What must be taken into
account?
All safety instructions in the user manual
The environmental, installation, and connection specifications in the user
manual
National and international regulations pertaining to laboratory work
Original spare parts, tools, and solvents made or recommended by
KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident insurance
companies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once they have been used, never re-use capillaries in other areas of the
HPLC system.
Only use a given PEEK fitting for one specific port and never re-use it for
other ports. Always install new PEEK fittings on each separate port.
Follow KNAUER or manufacturer's instructions on caring for the columns
More safety-relevant information is listed below:
flammability: Organic solvents are highly flammable. Since capillaries can
detach from their screw fittings and allow solvent to escape, it is prohib-
ited to have any open flames near the analytical system.
solvent tray: Risk of electrical shock or short circuit if liquids get into the
device's interior. For this reason, place all bottles in a solvent tray.
solvent lines: Install capillaries and tubing in such a way that liquids can-
not get into the interior in case of a leak.
leaks: Regularly check if any system components are leaking.
power cable: Defective power cables are not to be used to connect the
device and the power supply system.
self-ignition point: Only use eluents that have a self-ignition point higher
than 150 °C under normal ambient conditions.
Accessories Kit DAD 6.1L, DAD 2.1L, MWD 2.1L
AZURA

DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
Safety for Users 11
power strip: If several devices are connected to one power strip, always
consider the maximum power consumption of each device.
power supply: Only connect devices to voltage sources, whose voltage
equals the device's voltage.
toxicity: Organic eluents are toxic above a certain concentration. Ensure
that work areas are always well-ventilated! Wear protective gloves and
safety glasses when working on the device!
Where is use of the device
prohibited?
Never use the system in potentially explosive atmospheres without appro-
priate protective equipment. For further information, contact the Technical
Support of KNAUER.
Secure decommissioning At any time, take the device completely out of operation by either switching
off the power switch or by pulling the power plug.
Opening the Device The device may be opened by the KNAUER Technical Support or any com-
pany authorized by KNAUER only.
Signal Words
Possible dangers related to the device are divided into personal and material
damage in this user manual.
Lethal injuries will occur.
Serious or moderate injuries can occur.
Minor injuries can occur.
Device defects can occur.
Decontamination
Contamination of devices with toxic, infectious or radioactive substances
poses a hazard for all persons during operation, repair, sale, and disposal of
a device.
Life-threatening injuries
Health danger if getting in contact with toxic, infectious or radio-active sub-
stances.
Before disposing of the device or sending it away for repair, you are
required to decontaminate the device in a technically correct manner.
All contaminated devices must be properly decontaminated by a specialist
company or the operating company before they can be recommissioned,
repaired, sold, or disposed of. All materials or fluids used for decontamina-
tion must be collected separately and disposed of properly.
Decontamination Report Devices without a completed Decontamination Report will not be repaired.
If you would like to return a device to KNAUER, make sure to enclose a com-
pleted Decontamination Report with the device: http://www.knauer.net/
en/downloads/service.html