Koehler K13560 Guide

Operation and Instruction Manual
REV B
Koehler Instrument Company, Inc.
1595 Sycamore Avenue • Bohemia, New York 11716-1796 • USA
Toll Free: 1-800-878-9070 (US only) • Tel: [phone] • Fax: [phone]
http://www.koehlerinstrument.com • e-mail: [e-mail]
Petroleum Testing & Analysis Instrumentation • Custom Design & Manufacturing
K13560 / K13270
Automatic Colorimeter

CERTIFICATE OF CONFORMANCE
Portable Automatic Colorimeter
K13560 / K13270
This certificate verifies that part number K13560 / K13270, Portable
Automatic Colorimeter, was manufactured in conformance with the
applicable standards set forth in this certification.
Specifications: ASTM D156
ASTM D1209
ASTM D1500
ASTM D1544
ASTM D6045
ISO 4630
ISO 6271
DIN 6162
NF M 07-003
NF T 60-104
JIS K2580
This unit is tested before it leaves the factory, to ensure total functionality
and compliance to the above specifications and ASTM standards. Test and
inspection records are on file for verification.
Jesse Kelly
Application Engineer
Koehler Instrument Company

3
Table of Contents
Section 1 Specifications ........................................................................................................................ 7
Section 2 General Information............................................................................................................... 9
2.1 Safety information............................................................................................................................... 9
2.1.1 Precautionary labels .................................................................................................................. 9
2.1.2 Safety around source lamps .................................................................................................... 10
2.1.3 Chemical and biological safety ................................................................................................ 10
2.2 Overview of product.......................................................................................................................... 10
Section 3 Installation ............................................................................................................................ 13
3.1 Unpacking the instrument ................................................................................................................. 13
3.2 Operating environment ..................................................................................................................... 13
3.3 Front and back view.......................................................................................................................... 14
3.4 Power connections ........................................................................................................................... 15
3.5 Interfaces .......................................................................................................................................... 16
3.6 Cuvette compartments and cuvette adapters ................................................................................... 16
3.6.1 Cuvette compartments and adapter......................................................................................... 16
3.6.2 Installation of cuvette adapter Z............................................................................................... 17
Section 4 Start Up ................................................................................................................................. 19
4.1 Switch on the instrument, startup process........................................................................................ 19
4.2 Language selection........................................................................................................................... 19
4.3 Self-check ......................................................................................................................................... 19
4.4 Sleep mode....................................................................................................................................... 20
4.5 Power off the instrument................................................................................................................... 20
Section 5 Standard programs.............................................................................................................. 21
5.1 Overview........................................................................................................................................... 21
5.1.1 Tips for using the touch screen................................................................................................ 21
5.1.2 Use of the alphanumeric keypad .............................................................................................21
5.1.3 Main menu ............................................................................................................................... 22

4
Table of Contents
5.2 Instrument Setup...............................................................................................................................23
5.2.1 Operator ID...............................................................................................................................23
5.2.2 Sample ID.................................................................................................................................24
5.2.2.1 Sample ID with hand-held scanner method 1..................................................................25
5.2.2.2 Sample ID with hand-held scanner method 2..................................................................26
5.2.2.3 Import sample ID list........................................................................................................26
5.2.3 Security settings .......................................................................................................................27
5.2.3.1 Assign operator security level..........................................................................................28
5.2.3.2 Deactivate password .......................................................................................................30
5.2.4 Date and time...........................................................................................................................30
5.2.5 Sound settings..........................................................................................................................32
5.2.6 PC and printer ..........................................................................................................................32
5.2.6.1 Printer setup ....................................................................................................................33
5.2.6.2 Print data .........................................................................................................................34
5.2.6.3 Print data continuously ....................................................................................................34
5.2.6.4 Network setup..................................................................................................................35
5.2.7 Power Management .................................................................................................................38
5.3 Save, recall, send and delete data....................................................................................................38
5.3.1 Color values .............................................................................................................................38
5.3.1.1 Automatic/manual color value storage ............................................................................39
5.3.1.2 Recall color values from the measured data memory .....................................................39
5.3.1.3 Send color values from the measured data memory.......................................................41
5.3.1.4 Delete color values from the measured data memory.....................................................42
5.3.2 Control charts for data from the AQA Log................................................................................42
5.3.3 Data log (only K13560).............................................................................................................43
5.3.3.1 Automatic/manual saving of single and multi-wavelength measurements ......................44
5.3.3.2 Recall single and multi-wavelength measurements from the measured data memory ...44
5.3.3.3 Send single and multi-wavelengths from the measured data memory............................46
5.3.3.4 Delete single and multi-wavelengths from the measured data memory..........................47
5.3.4 Wavelength scan and time course (K13560 only)....................................................................47
5.3.4.1 Data storage from wavelength scan or time course ........................................................48
5.3.4.2 Recall stored data from wavelength scan or time course................................................48
5.3.4.3 Send data from wavelength scan or time course ............................................................49
5.3.4.4 Delete stored data from wavelength scan or time course ...............................................50
5.3.5 Trends ......................................................................................................................................50
5.4 Take and prepare samples................................................................................................................53

5
Table of Contents
5.5 Color measurement .......................................................................................................................... 54
5.5.1 Take a color measurement ...................................................................................................... 55
5.5.1.1 Touch-sensitive areas in measurement mode ................................................................ 55
5.5.1.2 Parameter setup options................................................................................................. 56
5.5.1.3 Change the color scale after a measurement ................................................................. 57
5.5.1.4 Change the measuring range after a measurement ....................................................... 58
5.5.2 Determination of the iodine color value....................................................................................58
5.5.3 Determination of the Hazen color value (Pt-Co or APHA method) .......................................... 58
5.5.4 Determination of the Gardner color value................................................................................ 59
5.5.5 Determination of the Mineral oil color value (ASTM D 1500 and ISO 2049)............................ 59
5.5.6 Determination of the Saybolt color number (ASTM D 156)...................................................... 59
5.5.7 Color determinations only by K13560 ...................................................................................... 60
5.5.7.1 Color determination according to the European Pharmacopoeia (EP) ........................... 60
5.5.7.2 Color determination according to the US Pharmacopoeia (USP) ................................... 63
5.5.7.3 Color determination according to the Chinese Pharmacopoeia (CP) ............................. 64
5.5.7.4 Determination of the Klett color value ............................................................................. 64
5.5.7.5 Determination of the AOCS Cc 13 e (Lovibond®) scale.................................................. 65
5.5.7.6 Determination of the Yellowness Index (ASTM D 1925)................................................. 65
5.5.7.7 Determination of the Hess-Ives color number................................................................. 65
5.5.7.8 Determination of the ADMI color number........................................................................ 65
5.5.7.9 Determination of the Acid wash color ............................................................................. 66
5.5.7.10 Determination of the ICUMSA color index .................................................................... 66
5.6 Measurement of color differences (K13560 only) ............................................................................. 67
5.6.1 Take a color difference measurement ..................................................................................... 67
5.6.1.1 View graph/table/values..................................................................................................69
5.6.2 Take a color difference measurement with stored reference values ....................................... 70
5.6.3 Add a reference to the reference list........................................................................................ 70
5.7 Photometry (K13560 only) ................................................................................................................ 71
5.7.1 Single Wavelength (absorbance, concentration and transmittance readings)......................... 71
5.7.1.1 Set up Single Wavelength mode..................................................................................... 71
5.7.1.2 Take single wavelength readings (single reading).......................................................... 73
5.7.2 Multi Wavelength mode – readings with more than one wavelength....................................... 73
5.7.2.1 Set the Reading mode at different wavelengths ............................................................. 73
5.7.2.2 Complete a reading in the Multi Wavelength mode ........................................................ 76
5.7.3 Time course of absorbance/transmittance............................................................................... 76
5.7.3.1 Time course setup parameters ....................................................................................... 76
5.7.3.2 Time course scan reading............................................................................................... 78
5.7.3.3 Analysis of time course data ........................................................................................... 78
5.7.4 Wavelength Scan mode – recording of absorbance and transmission spectrums .................. 79
5.7.4.1 Set up the wavelength scan............................................................................................ 80
5.7.4.2 Perform a wavelength scan ............................................................................................ 82
5.7.4.3 Work with reference scans.............................................................................................. 83

6
Table of Contents
Section 6 Advanced Operations ..........................................................................................................87
6.1 System Checks .................................................................................................................................87
6.1.1 Instrument Information .............................................................................................................87
6.1.2 Update the instrument software ...............................................................................................87
6.1.2.1 Software update with USB memory.................................................................................87
6.1.2.2 Software update with network connection.......................................................................88
6.1.3 Optical Checks .........................................................................................................................88
6.1.4 AQA Analytical Quality Assurance ...........................................................................................90
6.1.4.1 Standard Configuration....................................................................................................90
6.1.4.2 Perform AQA Standard Measurement.............................................................................91
6.1.5 Instrument backup....................................................................................................................92
6.1.6 Service menu ...........................................................................................................................93
6.1.7 Service time..............................................................................................................................93
6.1.8 Lamps operating time...............................................................................................................93
6.2 Toolbar ..............................................................................................................................................94
6.2.1 Log on ......................................................................................................................................94
6.2.2 Sample ID.................................................................................................................................94
6.2.3 Timer ........................................................................................................................................95
6.2.4 AQA..........................................................................................................................................95
6.2.5 Trends ......................................................................................................................................95
6.2.6 K13560/K13270 Website ..........................................................................................................95
Section 7 Maintenance..........................................................................................................................97
7.1 Cleaning requirements ......................................................................................................................97
7.1.1 Spectrophotometer...................................................................................................................97
7.1.2 Screen ......................................................................................................................................97
7.1.3 Cuvettes/cells ...........................................................................................................................98
7.2 Lamp replacement.............................................................................................................................98
7.3 Cell compartment (2) replacement..................................................................................................100
Section 8 Troubleshooting .................................................................................................................103
Section 9 Replacement Parts .............................................................................................................107
9.1 Accessories.....................................................................................................................................107
9.2 Replacement Parts..........................................................................................................................107

7
Section 1 Specifications
These are subject to change without notice!
Performance specifications K13560 K13270
Display mode
Color measurement, color difference
measurement, absorbance and
concentration
Color measurement
Color measurement 26 color ratios 5 color ratios
Colorimetric evaluation
All visual color ratios are calculated for standard light chart C and 2° standard
observers in accordance with DIN 5033. Colorimetric color values can be switched
to light type A, C, D65 and 2° or 10° standard observers.
Source lamp Halogen lamp
Wavelength range 320–1100 nm
Wavelength Accuracy ± 1.5 nm (wavelength range 340–900 nm)
Wavelength reproducibility ≤0.1 nm
Wavelength resolution 1 nm
Wavelength calibration Automatic
Wavelength range for color
measurement 380–720 nm steps of 10 nm
Scanning speed ≥8 nm/sec (in steps of 1 nm)
Spectral bandwidth 5 nm
Photometric measuring range ± 3 Abs (wavelength range 340–900 nm)
Photometric accuracy 5 m Abs at 0.0–0.5 Abs,
1 % at 0.50–2.0 Ext
Photometric linearity < 0.5 % to 2 Abs
≤1 % at > 2 Abs with neutral glass at 546 nm
Stray light < 0.1 % T at 340 nm with NaNO2
Data log
3000 color measurements,
100 color reference values,
1000 photometric measurements,
20 wavelength scans, 20 time scans
400 color measurements
Physical and environmental specifications
Width 350 mm (13.78 in)
Height 151 mm (5.94 in)
Depth 255 mm (10.04 in)
Earth 4200 g (9.26 lb)
Ambient operating requirements 10–40 °C (50–104 °F), maximum 80 % relative humidity (without condensate
formation)
Ambient storage requirements –40–60 °C (–40–140 °F), maximum 80 % relative humidity (without condensate
formation)
Additional technical data
Power connector via external power
supply
Input: 100–240 V/47–63 Hz
Output: 15 V/40 VA

8
Specifications
Interfaces
Use only shielded cable with maximum length of 3 m:
2× USB type A
1× USB type B
Use only shielded cable (for example STP, FTP, S/FTP) with maximum length of
20 m:
1× Ethernet
Housing rating IP40 (excluding interfaces and power supply)
Protection class Class I
Performance specifications K13560 K13270

9
Section 2 General Information
2.1 Safety information
Read through the entire user manual carefully before you unpack
the device, set up and put into operation. Pay attention to all
danger and caution statements. Failure to do so could result in
serious injury to the operator or damage to the equipment.
To make sure that the protection provided by this instrument is
not impaired, do not use or install this instrument in any manner
other than that specified in these operating instructions.
Note: Information that supplements points in the main text.
2.1.1 Precautionary labels
Read all labels and tags attached to the instrument. Personal
injury or damage to the instrument could occur if not observed.
For symbols attached to the device, corresponding warning notes
are found in the user manual.
DANGER
Indicates a potentially or imminently hazardous situation that, if not
avoided, results in death or serious injury.
WARNING
Indicates a potentially or imminently hazardous situation that, if not
avoided, may result in death or serious injury.
CAUTION
Indicates a potentially hazardous situation that may result in minor or
moderate injury.
NOTICE
Indicates a situation that, if it is not avoided, can lead to damage to
the device. Information that requires special emphasis.
This symbol may be attached to the device and references the operation- and/or safety notes in the user manual.
This symbol on the device is an indication of hot surfaces.
Electrical equipment marked with this symbol may not be disposed of in European domestic or public disposal
systems after 12 August 2005. In conformity with European local and national regulations (EU Directive
2002/96/EC), European electrical equipment users must now return old or end-of life equipment to the
manufacturer for disposal at no charge to the user.
Note: For return for recycling, please contact the equipment manufacturer or supplier for instructions on how to
return end-of-life equipment, manufacturer-supplied electrical accessories, and all auxiliary items for proper disposal.

10
General Information
2.1.2 Safety around source lamps
The source lamp is operated at high temperatures.
To prevent electric shock, disconnect the instrument from the
power source before replacing the lamp.
2.1.3 Chemical and biological safety
Normal operation of this device may require the use of chemicals
or samples that are biologically unsafe.
•Observe all cautionary information printed on the original
solution containers and safety data sheets prior to their use.
•Dispose of all consumed solutions in accordance with the
local and national regulations and laws.
•Select the type of protective equipment suitable to the
concentration and quantity of the dangerous material being
used.
2.2 Overview of product
The K13560 and K13270 instruments are VIS
spectral-photometers with wavelength ranges from 320 to
1100 nm. The instruments can perform a precise colorimetric
analysis in accordance with ISO/ASTM standards with a single
measurement and display the result in the form of classic color
systems such as iodine, Hazen or Gardner color values. The
instruments support multiple languages.
The K13560 is supplied with 26 color value calculations, while
the K13270 is supplied with five color value calculations (Iodine
color, Hazen color, Gardner color, Saybolt color and ASTM D
1500 color numbers).
WARNING
The manufacturer is not responsible for any damages due to
misapplication or misuse of this product including, without limitation,
direct, incidental and consequential damages, and totally excludes
such damages as permitted under applicable laws.
The user is solely responsible to identify critical application risks and
install appropriate mechanisms to protect processes during a possible
equipment malfunction.
CAUTION
Burn hazard. Allow the lamp(s) to cool for at least 30 minutes before
maintaining/replacing them.
DANGER
Potential danger with contact with chemical/biological substances.
Working with chemical samples, standards and reagents can be
dangerous.
Make yourself familiar with the necessary safety procedures and the
correct handling of the chemicals before use and read and follow all
relevant safety data sheets.

11
General Information
The K13560 contains the following programs and operating
modes in addition to the color measurement: single wavelength
mode, multi-wavelength mode, wavelength scan and time scan
mode. The digital measurements are displayed in the
dimensional units of concentration, absorbance or %
transmittance, making the K13560 universally suitable for lab
analysis.

12
General Information

13
Section 3 Installation
3.1 Unpacking the instrument
The following components are supplied as standard with the
K13560/K13270:
•K13560/K13270 spectrophotometer
•Dust cover
•USB dust cover, fitted as standard
•Table power supply with power cord
•Cuvette adapter Z, installed as standard
•Basic user manual
•CD-ROM with detailed operating instructions
Note: If any of these items are missing or damaged, please contact the
manufacturer or a sales representative immediately.
3.2 Operating environment
Observe the following points to allow the instrument to function
normally and give a long operating life.
•Position the instrument securely on a flat surface taking care
to remove any objects from under the device.
•The ambient temperature must be 10–40 °C (50–104 °F).
•The relative humidity should be less than 80 %; moisture
should not condense on the instrument.
•Leave at least a 15 cm clearance at the top and on all sides
for air circulation to avoid overheating of electrical parts.
•Do not use or store the device in extremely dusty, humid or
wet places.
•Keep the surface of the instrument, the cell compartment and
all accessories clean and dry at all times. Immediately
remove splashes or spilt materials on or in the instrument
(refer to Section 7).
WARNING
Electrical and fire hazards.
Only use the supplied benchtop power supply.
Only qualified experts may perform the tasks described in this section
of the manual, while adhering to all locally valid safety regulations.
NOTICE
Protect the instrument from extreme temperatures from heaters,
direct sunlight and other heat sources.

14
Installation
3.3 Front and back view
Figure 1 Front view
1USB port type A 3Touch screen
2Cuvette compartment cover 4On/off switch

15
Installation
Figure 2 Back view
3.4 Power connections
1. Connect the power cable to the benchtop power supply.
2. Plug the benchtop power supply cable into the back of the
instrument (Figure 2, page 15).
3. Insert the power cable plug into a mains socket
(100–240 V~/47–63 Hz).
4. Turn on the power switch next to the screen to activate the
power supply (Figure 1, page 14).
Note: If you do not intend to use the instrument for a long period,
disconnect it from the power supply.
Note: Make sure the socket you are using is easily accessible.
1Ethernet port 3USB port type A
2USB port type B 4Connection for benchtop power supply
WARNING
Electrical and fire hazards.
Only use the supplied benchtop power supply.

16
Installation
3.5 Interfaces
The instrument has three USB ports and one Ethernet port as
standard. They are located on the front and rear of the instrument
(Figure 1 Figure 2, page 15).
The USB type A ports are used for communications with a printer,
USB memory stick or keyboard. A USB memory stick can be
used to update the instrument software.
The USB type B port is used for communications with a PC. The
optional Hach Lange Online Data software must be installed on
the PC for this use.
A USB hub may be used to connect several accessories at a
time.
Note: USB cables must not be longer than 3m.
These USB ports permit data to be exported to a printer or PC
and also allow the instrument software to be upgraded (refer to
section 6.1.2, page 87). The Ethernet port supports real-time data
transfer in local networks, LIMS systems or SC controllers. Only
use a shielded cable (e.g. STP, FTP, S/FTP) with a maximum
length of 20 m for the Ethernet port.
3.6 Cuvette compartments and cuvette adapters
3.6.1 Cuvette compartments and adapter
Open the cuvette compartments by sliding the cuvette
compartment cover to the left.
The cover lowers to the side next to the cuvette compartments.
Note: If there are long intervals between uses, close the cuvette
compartment cover to protect the optics of the instrument from dust and
impurities.
The instrument has two cuvette compartments (Figure 3). Only
one cell type at a time can be used for a reading.
Cell compartment (1) for:
•11 mm round cuvettes
Note: Insert cuvette adapter Z into the cuvette compartment (2).
Table 1 Interfaces
Interfaces Description
USB (Type A) This USB port can be used to connect a printer, a USB memory stick or a keyboard.
USB (Type B) This USB port is intended only for the connection between the instrument and a PC (when the
relevant software is installed).
Ethernet
The Ethernet port is intended for data transfer to a PC without installed software or in a local
network . Only use a shielded cable (e.g. STP, FTP, S/FTP) with a maximum length of 20 m for
the Ethernet port.

17
Installation
Cell compartment (2) for:
The following cell types can be used in cell compartment (2).
•Without cuvette adapter Z in the cuvette compartment (2),
you can insert 50 mm cuvettes.
•With cuvette adapter Z: 10 mm square cuvettes.
Note: These cuvettes must be inserted with cuvette adapter Z.
Note: In the event of severe contamination, you can replace the cuvette
compartment (2) (see chapter 7.3, page 100).
3.6.2 Installation of cuvette adapter Z
1. Open the cuvette compartment.
2. Insert cuvette adapter Z into the cuvette compartment (2) so
that the arrow on the cuvette adapter is pointing toward the
cuvette compartment (1) (Figure 3).
Note: The arrow on the cuvette adapter indicates the direction of the
light beam path.

18
Installation
Figure 3 Cuvette compartments and cuvette adapter Z
1Cuvette compartment (1) for round cuvettes 2Cuvette compartment (2) for square cuvettes, cuvette
adapter Z installed