Labomed L POL Series User manual

Part No: 9151000-795
Issue 1.3
Printed on April,2019
Lx POL
Research Microscopy
User Manual
To ensure proper use of this instrument as well as to avoid injury while operating
Instrument, understanding this manual completely before use is highly recommended.

TABLE OF CONTENTS
Lx POL
1. INTRODUCTION 1
2. SAFETY INFORMATION 2-5
a. General Instructions
b. Symbol Used
c. Maintenance & Care
d. Health Risks
e. Electric Data
3. Lx POL BINOCULAR 6
4. Lx POL TRINOCULAR 7
5. UNPACKING YOUR MICROSCOPE 8
6. STANDARD COMPONENTS 9
7. OPTIONAL ACCESSORIES & INSTALLATION 10-11
8. DETAILED INTRODUCTION OF ASSEMBLIES 12-14
a. Abbe Condenser
b. Round Stage
c. Centerable revolving nose piece
d. Objectives
e. Eye pieces
f. Bertrand Lens
9. INITIAL SETUP 15-16
a. Observation Head
b. Eye Pieces
c. Mounting the Day Light (Blue) Filter
d. Connecting Power Cords
10. CENTRATION 17-18
a. Preparation for centration
b. Kohler illumination centration
c. Abbe Condenser centration
d. Objective centration
e. Extinction adjustment
f. Conoscopical adjustment
11. SUMMARY OF POLARISED OBSERVATION PROCEDURE 20

Lx POL
12. SUMMARY OF POLARISED OBSERVATION PROCEDURE 21-24
a. Placing the specimen on stage
b. Adjusting the focus
c. Adjusting the Interpupillary Distance
d. Adjusting the Diopter
e. Adjusting the Condenser position and Aperture Iris Diaphragm
f. Switching the objectives
g. Using the 100x Immersion Objective
13. TROUBLESHOOTING GUIDE 25-26
14. SPECIFICATIONS 27

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Lx POL
1INTRODUCTION
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12. SUMMARY OF POLARISED OBSERVATION PROCEDURE 21-24
a. Placing the specimen on stage
b. Adjusting the focus
c. Adjusting the Interpupillary Distance
d. Adjusting the Diopter
e. Adjusting the Condenser position and Aperture Iris Diaphragm
f. Switching the objectives
g. Using the 100x Immersion Objective
13. TROUBLESHOOTING GUIDE 25-26
14. SPECIFICATIONS 27
The Lx POL is a research polarizing microscope reflecting a modern design as well as the latest in optical
and mechanical advancements. Designed for professionals, this microscope offers many features and
functions for a diverse set of applications. It is a truly professional polarizing Microscope that meets, and
indeed exceeds the quality of some of the competing Microscopes. Here are a few points highlights
the benefits of the Lx POL:
•Extra clarity and contrast is provided through a 360 degree rotatable viewing body inclined at 30
degree with IPD adjustments.
•The pressure die cast stand consists of ball bearing, frictionless sideways focusing to avoid any loss
motion.
•The sturdy new stylish design provides a high degree of comfort as well as stability.
•The high powered objectives are spring loaded to prevent accidental damage to specimen slides.
•The highly precision Parfocalized & Parcentered reverse angle quadruple nose piece has the provision
of centering for all objectives.
•The round ball bearing Rotatable Stage has smooth 360degree travel and has 1 degree graduations
on the scale which provides accurate location of the specimen.
•High power illumination is delivered through our well crafted Universal Power Supply and operates
on 100V- 240V AC constant input.
•Halogen bulb (6V-20W) has an average life span of upto 2,000hours.
•The Lx POL is equipped with a Abbe Condenser N.A. 1.25 to attain for brighter illumination levels. An
iris diaphragm is also provided for better resolution and contrast control.
•High quality Polarize & Analyzer filters for perfect extinction level.
•Polarizer: This is provided below the condenser and is 360 degree graduated and lockable in any
desired position.
•Analyzer: The advances Analyzer module is located below the viewing tube and provides specific
cross polarization through the full 90 degree quadrant.
•A center adjustable focusable Bertrand lens is a standard feature in the module which provides
conoscopic observation.
•A set of high quality compensator for advanced polarized observation include Gypsum full wave
length Quartz Wedge and A Mica 1/4 wave plate.

SAFETY INFORMATION
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Lx POL
2
AGENERAL INSTRUCTIONS
9151000-795 Lx POL
Issue 1.3
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!
BSAFETY SYMBOLS
CMAINTENANCE AND CARE
1. A microscope is a precision instrument with delicate glass components, please handle with care.
2. Do not use the microscope where it is subjected to direct sunlight, high temperature, humidity, dust and
vibrations.
3. The microscope is ventilated by natural convention. Be sure to leave enough space (10 cm or more)
around body when installing the unit.
4. Arm handle is provided for carrying the microscope.
To prevent damage, do not hold the microscope by the stage or observation tube. Be sure to remove the
specimen from the stage clip while transporting unit to avoid damage to the speciemen slide.
The following symbols are found on the microscope. For optimal use, it is recommended that users
understand these symbols and always use the eqipment as prescribed:
Symbols Explanation
The surface has a tendency to heat up and should not be touched unless
system has completely cooled down.
Before use, carefully read the instruction manual. Improper use could
result in injury to the user and/or damage to the euipment.
Warning against risk of electric shock.
Main switch is ON.
Main switch is OFF.
If the microscope is used in a manner not specified by this manual, the safety of the user may not be
warranted. In addition, the equipment may also suffer damage. Always use the equipment as outlined in
this instruction manual.
I) GENERAL CLEANING
Your Microscope has been engineered for a long and safe operational life with the least amount of mainte-
nance required. In general, routine maintenance is limited to keeping the microscope working parts lubri-
cated and optics clean.
•Clean all glass components by wiping gently with cleaning cloth provided. To remove fingerprints or oil
smudges, wipe with cleaning cloth slightly moistened with a mixture of petroleum (85%) and isopropa-
nol (15%).
Since solvents such as petroleum and isopropanol are highly flammable, they must be handles carefully. Be
sure to keep these chemicals away from open flames or potential sources of electrical sparks- for example,
electrical equipment that is being switched “ON” or “OFF”. Also remember to always use these
chemicals only in a well-ventilated room.
•Do not attempt to use organic solvents to clean the microscope components other than the glass compo
-nents. To clean non-glass components, use a lint-free, soft cloth slightly moistened with a diluted neut
-ral detergent.

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Wipeusingaspiralmovement Do notusea zig-zagmotion
3. Do not disassemble any part of the microscope as this could result in malfunction or mitigated perfor-
mance.
4. When not using the microscope, ensure that the frame is fully cooled before storing the unit in a dry
locker or covering with dust cover (provided).
5. To clean the Condenser, fully loosen the securing thumb screw (1) and remove the condenser then,
wipe the front lens of the condenser with optical cleaning solution (mixture suggested above) and lens
tissue.
The Condenser Abbe can be re-attached in its seat, by tightening securing thumb screw, and raising
condenser bracket to desired position. (As shown in picture)
6. Always cover the Microscope with the provided dust cover when not in use
II) OPTICAL CLEANING
1. The objective have been adjusted for a tight fit to prevent any damage during transportation. To
remove an objective, rotate it counterclockwise while gripping it with a rubber sheet, etc. to avoid any
slip page.
2. To clean the lens surfaces, remove dust using a soft brush or gauze (compressed air dust cans are
ideal). For removing finger marks or grease, soft cotton cloth or lens tissue lightly moistened
with cleaning solution ( 85% petroleum ether and 15% isopeopanol) should be used. For cleaning the
optics, use Meathanol. Observe sufficient caution in handling Methanol. Place the Objective and/ or
eyepieces on a dust free surfaces (e.g. aluminum foil). All other optical components to be cleaned
should be as accessible as possible.
3. Blow all loose dust particles away with a dust blower.
4. Remove all water-soluble dirt with distilled water. If this is unsuccessful repeat using a solution of
diluted hand soap liquid. Remove any remaining residue with a dry cotton swab.
5. To remove oil, use a solution of diluted hand-soap liquid initially. If this does not produce a satisfac-
tory result, repeat the cleaning using a solvent (Optical Cleaning Solution 85% petroleum ether and
15% isopropanol).
6. Grease must always be removed using a solvent.
7. Cleaning is achieved by using a spiral motion from the center to the rim. Never wipe using zig-zag
movements as this will only spread the dirt. With larger optical surfaces (e.g. tube lenses) the spiral
motion starts initially at the rim before to the middle and is only then followed by a center to rim
cleaning motion. Normally several spiral wipes are recommended.
We recommend pure, volatilepetroleum ether or Optical Cleaning Solution as explained in point 3
above.
III). CLEANING OF PAINTED SURFACES
Avoid the use of any organic solvent (e.g. thinner, xylene, ether, alcohol etc.) for cleaning of painted
surfaces of the instrument. Painted surfaces can be cleaned with a very lightly moistened micro fiber
cloth. Loose dust and dirt can be removed using a brush of soft hair used exclusively for this purpose.

Lx POL
DHEALTH RISKS
EELECTRICAL DATA
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This microscope has an ergonomic design that ensures minimum exertion of the user. However, some of
the risks that the user should keep in mind are:
I) RISK OF INFECTION:
After the microscope has been used for observation of a specimen containing bacteria, clean all parts
coming in contact with the specimen to prevent infection.
1. Be sure to remove the specimen before moving this product.
2. In case the specimen is damaged by erroneous operation, it is important to clean all surfaces that may
come in contact with the specimen.
II) ELECTRICAL HAZARDS:
To avoid potential electrical hazards when replacing halogen bulb turn the microscope’s main switch to
the OFF position and disconnect power cord from wall outlet in advance. Whenever you replace your
microscope bulb, allow lamp socket and bulb to cool before touching.
I) GENERAL INSTRUCTIONS
1. Install microscope on a sturdy, level table or bench and avoid any restriction of air vents in the base of
the unit. Do not place microscope on a flexible surface as this could result in blocking the air vents and
cause overheating.
2. Always use the power cord provided by LABOMED. If the proper power cord is not used, product safety
performance cannot be warranted.
3. When installing the Microscope, route the power cord away from the microscope frame, Should the
power cord come in contact with the Microscope base, the power cord could melt due to overexposure
heat.
4. Always ensure that the grounding terminal of the Microscope and that of the wall outlet are properly
connected. If the unit is not grounded. LABOMED can not warrant electrical safety.
5. Never allow metallic objects to penetrate the air vents of the Microscope frame as this could result in
user injury and damage to the Microscope.
6. After operation of Microscope, be sure to disconnect power cord from connector socket of the Micro-
scope or from the wall power outlet.

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Fig. 1
Fig. 2
1
23
1
2
3
II) BULB REPLACEMENT
III) INSTALLING OR REPLACING THE FUSE
1. Before attaching the lamp bulb, remove the parts that may drop
such as the filter and specimen from the Microscope frame, and
place the Microscope on its back so that the bottom plate is
exposed.
2. Pull the lock knob (1) on the bottom to open lamp housing door
(fig.1).
3. Hold the Halogen bulb (2) without taking it out of the polyeth-
ylene bag so as not to taint the bulb with fingerprints and push
the bulb into the pin holes on the socket (3). After attaching,
remove the polyethylene bag.
4. With the lock knob pilled out, close the lamp housing door, then
push the lock knob back to lock the cover. Always use the desig-
nated bulb. Using a bulb other than those specified by LABOMED
may lead to a fire hazards. Fingerprints or stains on the lamp
bulb reduce its life. If contamination occurs, wipe bulb surface
with a cloth slightly moistened with alcohol.
Applicable Bulb: 6V20W Halogen Bulb P/N CX-013
Caution: For Fuse Replacement
Set the main switch to “O” (OFF), disconnect the power cord from
the wall outlet.
Before replacing the fuse, remove the parts that may drop such as
the filter and specimen from the microscope frame. Turn around
the microscope to its back so that the AC inlet is visible.
1. Use a flat head screw driver to open the fuse holder (1).
2. The fuse tray will come out with (2) live fuse and (3)spare fuse.
Do not pull out the fuse tray with force as it is locked and will
not be out completely.
3. Replace the primary fuse (2) with the spare fuse.
4. Engage the fuse tray back in.
Always use the designated Fuse. Using a fuse other than those
specified by LABOMED may lead to a fire hazards.

Lx POL BINOCULAR
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Lx POL
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Binocular viewing tube 30° inclined
Eyepieces
Intermediate Analyzer Kit
Bertrand Lens centering screw
Centerable Revolving Nosepiece
Polarizing Objectives
Specimen Holder
Vernier Scale
Polarizer
Koehler mount
Coarse and
fine focus knob
Polarizer locking screw
Bertrand lens focusing knob
Bertrand lens on/off
Allen keys for
objective centering
Parking for additional
wave plates (2)
Analyzer on/off
Stage locking screw
Circular stage
with vernier
Port of wave plate
Day light blue filter
Abbe Condenser
Condenser Centering Screw

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Lx POL
Lx POL TRINOCULAR
4
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Eyepieces
Centerable Revolving Nosepiece
Polarizing Objectives
Koehler mount
Coarse and
fine focus knob
Stage locking screw
Circular stage
with vernier
Port of wave plate
Analyzer on/off
Parking for additional
wave plates (2)
Allen keys for
objective centering
Trinocular viewing tube, 30° inclined
Bertrand lens focusing knob
Bertrand lens on/off
Intermediate Analyzer Kit
Bertrand Lens centering screw
Specimen Holder Vernier
Scale
Abbe Condenser
Polarizer
Day light blue filter
Polarizer locking screw
Condenser Centering Screw

UNPACKING YOUR MICROSCOPE
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Lx POL
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Power Cord
Microscope Arm
Observation Head
Eye Pieces

STANDARD COMPONENTS
After removing your microscope from its packaging, make sure that all of the following contents are present.
“Please note that the contents of your microscope may vary as the optional configuration, contrasting
method or viewing body opted for may not be of the standard configuration highlighted here”.
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Lx POL
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Daylight (blue) filter
Power Cord
Allen Wrench 3mm
Polarizing attachment
Paired Eyepieces
6V 20W Halogen bulb
Binocular viewing tube 30° inclined
Eyepieces
Intermediate Analyzer Kit
Bertrand Lens centering screw
Centerable Revolving Nosepiece
Polarizing Objectives
Specimen Holder
Vernier Scale
Polarizer
Koehler mount
Coarse and
fine focus knob
Polarizer locking screw
Bertrand lens focusing knob
Bertrand lens on/off
Allen keys for
objective centering
Parking for additional
wave plates (2)
Analyzer on/off
Stage locking screw
Circular stage
with vernier
Port of wave plate
Day light blue filter
Abbe Condenser
Centering Screw

SYSTEM DIAGRAM OF OPTIONAL ACCESSORIES
ABBE CONDENSER 0.6
ABBE CONDENSER 1.25 POLARIZING KIT
WF 16xWF 10x WF 20x
TRINOCULAR HEADBINOCULAR HEAD
RP 4x RP 10x RP 40x (SL)
RP 20x RP 50x (SL, OIL) RP 100x (SL, OIL) RP PH 10x RP PH 20x RP PH 40x (SL) RP PH 100x (SL, OIL)
RP FL 4x RP FL 10x RP FL 40x (SL)
RP FL 20x RP FL 50x (SL, OIL) RP FL 100x (SL, OIL) RP 100x (OIL, IRIS)
HALOGEN BULB LED MOUNT RECHARGEABLE BATTERY
BLUE FILTER GREEN FILTER
ATLAS CMOS CAMERACMB CAMERA ADAPTER IVU TOUCH SCREEN VEGA CMOS CAMERA
PHASE TURRET
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Lx POL
OPTIONAL ACCESSORIES
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Fig. 3
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Lx POL
OPTIONAL EYEPIECES
2
INSTALLATION AND OPERATION OF OPTIONAL ACCESSORIES
CAMERA MODULE SYSTEM
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1. Mount the Video adapter 1/2” on Trinocular observation head.
2. Mount Camera Module System on Video adapter.
10X eyepieces are provided. To replace:
1. Pull out the 10x eyepieces out from the observation heads ocular
tube.
2. Insert desired eyepieces in empty ocular tube.
Fig. 6
Fig. 5
Fig. 4

Lx POL
DETAILED INTRODUCTION OF ASSEMBLIES
8
ABBE CONDENSER
1
POLARIZER
2
ROUND STAGE
2
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Fig. 7
Fig. 9
Fig. 8
LAB OMED
U SA
RP POL PLAN ACHRO
71.0/56.0/X04
9124040
2
1
3
Top Lens
Iris Shifter
Lx POL Abbe condenser is has following important basic require-
ments i.e.
1. Stain free optical system.
2. Rotate knurled ring to open and close IRIS Diaphragm for
respective Objective magnification in order to match numerical
aperture.
This high quality primary polarizer (1) is 360 degree rotatable
with fiducial marking (2) for quick identification of Polarized/
Cross Polarized positions fig.8
It is ideally located below condenser and with a lockable mecha-
nism (3) fig. 8(b) to stop polarizer filter at desired position for
comfortable observation experience.
Lx POL Polarizer is also equipped with a mechanism of swing
in/out the polarizer from the light path
Lx POL round stage has both the key feature of rotatability &
centerability (Fig.9). Lx POL round stage is equipped with the key
features of rotatability and centerability (Fig 9). It provides a 360
degree circular rotation which allows the user to study the orien-
tation by coinciding the objective centration with the microsc-
cope’s optical axis. This makes the center of rotation coincide
with the center of field of view. Rotatibility allows the user to
observe the specimen in the diagonal position (the brightest
position of anisotropy).
It is equipped with a vernier scale to measure the accuracy of 0.1
degree and locking provision to stop the stage at desired location.
Lx POL round mechanical stage is based on hard steel ball bearing
which provides even and smooth jerk free motion across 360
degree.

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Lx POL
CENTERABLE REVOLVING NOSE PIECE
OBJECTIVES
4
5
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Fig. 11
Fig. 10
1
Fig. 10-a
3
2
Quartz Wedge
1-4 Order
4
LABO MED
US A
RP POL PLA NA CHRO
40 X / 0. 6 5 / 0 .17
9124 040
Lx POL comes with a centerable revolving nose piece to compen-
sate optical axis of each objective as they vary from one assembly to
another. Lx POL Nose Piece is fully equipped with centering mecha-
nism for each microscopic optical axis so that the specimen remains
in the center when the stage is rotated. Movement of the turret is
based on hard steel ball bearing which provides smooth and jerk
free motion around 360 degree.
This nose piece is highly precision parfocalized and par centered.
Fig. # 10
1. Objective Centering Allen Screws
2. Nose Piece Cover
3. Port for Lamda Plate
Centerable revolving turret is also equipped with a Nose Piece
cover which provides a click stop lockable port for Gypsum Lambda
wave plates fig. 10-a. This feature provides comfort while using
different Lambda Wave Plates. It is ideally located in between
specimen & the Analyzer. It allows to introduce compensator &
retardation plates between the cross polarizer which can enhance
optical path differences in the specimen details.
Lx POL objectives are free from stress & both types of ‘strain’ i.e.
glass characteristics during various stages of the assembly like
cementing or mounted in close proximity with tightly fitted
frames. Lx POL objectives are assembled only after passing the
strict testing. Lx POL comes with standard 4x, 10x, 40x objectives
that solve the purposes of viewing specimen in conoscopic &
orthoscopes modules. These objectives are anti-refraction coated.
Some other objectives like 20x & 100x are also available.

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Lx POL
6
7
EYE PIECES
BERTRAND LENS
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Fig. 13
Fig. 14
1
2
3
4
5
6
2
3
Fig. 12
Lx POL comes with a pair of Eye Pieces. One of the eyepieces has a
crosshair reticule to mark the center of the field of view and the
second for normal viewing. Orientation of the Eye Piece with
respect to the Polarizer and Analyzer is ensured by the eye tube
lower that slides into the observation Bino Body tube. These eye
pieces also have a focusing mechanism for diopter correction &
foldable eye guards to prevent ambient light from entering into
your line of vision.
Lx POL comes with a Bertrand Lens that projects an interference
pattern formed at the objective rear focal plane into focus at the
microscope image plane. It is capable of examining the objective
rear focal plane, to ensure exact adjustment of the illumination
aperture diaphragm and to view interference images. These images
form in the objective rear focal plane when an optically anisotropic
specimen is viewed between cross polarize using a high numerical
aperture objective / condenser combination. It is ideally located
between analyzer and eye pieces for an easy in and out movement
from the light path. Lx POL Bertrand Lens is also centerale with
respect to the optical axis of the microscope and has focusable
mechanism for a comfortable viewing experience.
Fig. # 13 & 14
1. Bertrand Lens Focusing Knob
2. Bertrand Lens Operating Lever
3. Analyzer Operating Lever
4. Gypsum wave Plate
5. Allen Wrench for Objective centering
6. Allen screw for Bertrand Lens Centering.
Fig. 13: Bertrand Lens lever (2) in out “O” position i.e. away from
light path.
Analyzer Lever (3) in out “O” position i.e. away from light
path.
Fig. 14: Bertrand Lens lever (2) in “BL” position i.e. in light path.
Analyzer Lever (3) in “A” position i.e. in light path.

Install the observation head using the following procedure:
Refer Fig. 15
1. Using allen wrench 3mm (provided), loosen the Head Locking
Screw (1) and remove the dust cover cap provided in dovetail
cavity as well as on observation head dovetail.
2. Mount the Analyzer by engaging the dovetail provided on the
attachment into the dovetail cavity on the microscope arm and
tighten the Head Locking Screw (1) see figure 15.
3. Remove the Dust Cover Cap from Observation Head Dovetail
and mount the Observation Head by engaging the dovetail
provided at the bottom of the head into the dovetail cavity on
on the analyzer attachment.
4. Tighten the Head Locking Screw (2) after positioning the Obser-
vation head as desired. See figure 15.
Insert the eyepieces into the ocular tube of Observation Head
using following procedure:
1. Remove the protective caps from the observation tube.(Fig. 16)
2. Insert eyepieces into the ocular sleeve for use (Fig. 17).
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Lx POL
INITIAL SETUP
9
OBSERVATION HEAD
1
EYEPIECES
3
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Fig.15
Fig.16
1
2
Fig.17

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Lx POL
STAGE CLIPS
4
CONNECTING POWER CORD
5
MOUNTING THE DAYLIGHT (BLUE) FILTER
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Fig. 19
Fig. 20
On/Off SwitchElectrical outlet
Fig. 18
This filter modifies the color of observation light into a natural (day-
light) color.
Place Blue filter on Koehler Mount.
Fix Stage Clips on round rotatable stage for holding the POL speci-
men.
Attach the Power cord and plug it into a grounded electrical outlet.
1. Flip the main switch to “I” (ON) as shown in Figure 20.
2. Rotating the light intensity adjustment knob in the direction of
the arrow increases brightness and rotating knob in the opposite
direction decreases brightness. The intensity bar next to the knob
indicates the direction of the intensity level.
Note: Never use an adapter between the power cord and the power
source; it will render the microscope grounding feature ineffective.

Lx POL
17
10 CENTRATION
PREPARATION FOR CENTRATION
1
KOEHLER ILLUMINATION CENTRATION
2
ABBE CONDENSER CENTRATION
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Step: 1
Disengage the Analyzer and Bertrand Lens by switching the operat-
ing lever to “O” position as shown in Fig. 13. Fully open the aperture
diaphragm of the condenser by rotating its ring to the extreme left.
Lower the microscope stage. Place a POL specimen on the stage.
Swing in the 10x objective into working position. Raise the Micro-
scope stage using the coarse adjustment knob until you reach its
positive stop. Use the fine adjustment knob to bring the POL speci-
men into focus by lowering down the stage.
Step: 2
Swing in the 10x objective into working position. Flip in top lens of
condenser in light path. Close the Koehler field diaphragm so that
its closed isirs leaves image is present within the field of view. Use
the condenser focusing knob to bring the image into sharp focus.
Operate the condenser centering screws simultaneously to Center
the image of the field diaphragm Ref. Fig 22 (a), (b), (c). After
centration open the field diaphragm until the Image (iris leaves)
disappears just beyond the field of view.
Step:3
Look through 10x Objective & Eye Pieces cross hair scale. Close the
condenser diaphragm until approximately 20-25% of the iris leaves
fill the field of view. Adjust the centering of the diaphragm by main-
taining condenser centering screws simultaneously with reference
to Eye Piece cross hair scale Ref. Fig.23 (a).
Note: When changing the objective, adjust the condenser field
diaphragm with respect to each objective.
Fig. 23
Fig. 21
Fig. 22
Fig. 23 (a)
(a) (b) (c)
22 22 22