Labomed Lx 500 User manual

To ensure proper use of this instrument as well as to avoid injury while operating
instrument,understandingthismanualcompletelybeforeuseishighlyrecommended.
LABOMED
User Manual
Lx 500
Research Microscopy

CONTENTS
9 ASSEMBLY 12
12 TROUBLESHOOTING GUIDE 19-20
13 SPECIFICATIONS 21
11 DETAILED OBSERVATION PROCEDURE 14-18
10 SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE 13
8 INITIAL SETUP 11
7 OPTIONALACCESSORIES 9-10
6 STANDARD COMPONENTS 8
5 Lx 500 ERGONOMIC 7
4 Lx 500 TRINOCULAR 6
3 Lx 500 BINOCULAR 5
2 SAFETY INFORMATION 2-4
1 INTRODUCTION 1

The Lx 500 is a research microscope reflecting a modern design as well as the latest in optical and mechanical
advancements. Designed for professionals as well as clinicians, this microscope offers many features and functions for a
diversesetofapplications.HereareafewpointshighlightingthebenefitsoftheLx500:
-Extraclarityandcontrastisprovidedthrougha360°rotatableBinocularbodyinclinedat30°withIPDadjustments.
-Thepressurediecaststandconsistsofballbearing,frictionbasedfocusingmechanismtoavoidanylossinmotion.
-Thesturdynewstylishdesignprovidesahighdegreeofcomfortaswellasstability.
-Thehighpoweredobjectivesarespringloadedtopreventaccidentaldamagetospecimenslides.
-Thereverseanglequadruplenosepiecehasacomfortableribbedgripforeasyrotationthatalsosafeguardstheturret
systemagainstanydamage. Allpositionsarepar-centeredandpar-focalisedensuringthehighestlevelofaccuracy.
-Theballbearingmechanicalstageallowssmoothtravelovera76x50mmareawithspringloadedstageclampsforretaining
thespecimenattheexactpositiondesired. A 0.1mmvernierscaleprovidesaccuratelocationofspecimenarea.
- HighpowerilluminationisdeliveredthroughourwellcraftedUniversalPowerSupplyoperatablewithinrangeof100V-240V
ACinput. Thisensurescontinuousoperationevenunderfluctuatingvoltages.
- OurHalogenbulb(6V-30W)hasanaveragelifespanofupto500hours.
- TheLx500comesequippedwitharemovablefliptop N.A.1.25Abbecondenserwithasphericlensforbrighterillumination
levels,andanirisdiaphragmforbetterresolutionandcontrastcontrol.
INTRODUCTION
1
1

1. After the microscope has been used for observation of a specimen
containing bacteria, clean all parts coming in contact with the specimen
topreventinfection.
¡Besuretoremovethespecimenbeforemovingthisproduct.
¡Incase thespecimen isdamaged byerroneous operation,it isimportant
tocleanallsurfacesthatmayhavecomeincontactwiththespecimen.
Applicable bulb 6V30W Halogen bulb P/N EL-455
3. Installmicroscopeonasturdy,leveltableorbenchandavoidanyrestrictionof airventsinthebaseoftheunit.
Donotplacemicroscopeonaflexiblesurface,asthiscouldresultinblockingtheairventsandcauseoverheating.
4. Always use the power cord provided by LABOMED. If the proper power cord is not used, product safety
performancecannotbewarranted.
5. When installing the microscope, route the power cord away from the microscope frame. Should the power cord
comeincontactwiththemicroscopesbase,thepowercordcouldmeltduetooverexposuretoheat.
6. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If
theunit isnotgrounded,LABOMEDcannotwarrantelectricalsafety.
7. Neverallowmetallicobjectstopenetratetheairventsofthemicroscopeframeasthiscouldresultinuserinjuryand
damagetothemicroscope.
8. After operationofmicroscope,be sure todisconnectpower cord fromconnectorsocketof themicroscope or from
thewallpoweroutlet.
Fig. 1
Safety Symbols
Symbol Explanation
This surface has a tendency to heat up and should not be touched unless
system has completely cooled down.
Before use, carefully read the instruction manual. Improper use could result in
injury to the user and/or damage to the equipment.
Main switch is ON.
Warning against risk of electric shock.
Main switch is OFF.
The following symbols are found on the microscope. For optimal use, it is recommended that users understand
these symbols and always use the equipment as prescribed.
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SAFETY INFORMATION
2
2
Lx 500

1. Amicroscope isaprecisioninstrumentwith delicateglasscomponents.
Pleasehandlewithcare.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature,humidity, dust and vibrations. (For the operating
conditions,seechapter14,"SPECIFICATIONS")
3. The microscope is ventilated by natural convection. Be sure to leave
enoughspace(10cmormore)aroundbodywheninstallingtheunit.
4. Armhandleisprovidedforcarryingthemicroscope.
To prevent damage, do not hold the microscope by the stage or
observation tube.
Be sure to remove the specimen from the stage clip while
transporting unit to avoid damage to the specimen slide.
1
2
Getting Started
1. Clean all glass components by wiping gently with cleaning cloth provided. To remove fingerprints or oil smudges,
wipewithcleaningclothslightlymoistenedwithamixtureofpetroleum(85%)andisopropanol(15%).
Since solvents such as petroleum and isopropanol are highly flammable, they must be handled carefully.
Be sure to keep these chemicals away from open flames or potential sources of electrical sparks - for
example, electrical equipment that is being switched “ON” or “OFF”. Also remember to always use these
chemicals only in a well and ventilated room.
2. Donotattemptto use organic solventstoclean the microscope componentsotherthanthe glass components.To
cleannon-glasscomponents,usealint-free,softclothslightlymoistenedwithadilutedneutraldetergent.
3. Do not disassemble any part of the microscope as this could result in
malfunctionormitigatedperformance.
4. When not using the microscope, ensure that the frame is fully cooled
before storing the unit in a dry locker or covering with a dust cover
(provided).
5. Tocleanthecondenser,fullyloosenthesecuringknobsandremovethe
condenser by lowering condenser bracket then, wipe the front lens of
thecondenserwithopticalcleaningsolution(mixturesuggestedabove)
andlenstissue.
The condenser can be re-attached by replacing the condenser in its
seat, tightening securing screws, and raising condenser bracket to
desiredposition.
Fig. 2
Fig. 3
Maintenance and Storage
!
3
Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may not be warranted. In
addition, the equipment may also suffer damage.Always use the equipments as outlined in this instruction manual.
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4Care & Maintenance
Your microscope has been engineered for a long and safe operational life with the least amount of maintenance
required. In general, routine maintenance is limited to keeping the microscopes working parts lubricated and optics
clean.Alwayscoverthemicroscopewiththeprovideddustcoverwhennotinuse.
Optical Cleaning:
1. The objectives have been adjusted for a tight fit to prevent any damage during transportation. To remove an
objective,rotateitcounterclockwisewhilegrippingitwitharubbersheet,etc.toavoidanyslippage.
2. To clean the lens surfaces, remove dust using a soft brush or compressed air (cans available at your local
electronics store). For removing finger marks or grease, soft cleaning cloth or lens tissue lightly moistened with
cleaning solution (85% petroleum ether and 15% isopropanol) should be used. For cleaning the objective optics,
use Methanol. Observe sufficient caution in handling Methanol. Place the objectives and/or eyepieces on a dust-
freesurface(e.g.aluminumfoil).Allotheropticalcomponentstobecleanedshouldbeasaccessibleaspossible.
3.. Blowallloosedustparticlesawaywithcompressedairorminidustblower.
4. Remove all water-soluble dirt with distilled water. If this is unsuccessful repeat using a solution of diluted hand
soapliquid.Removeanyremainingresiduewithadrycottonswab.
5. To remove oil, use a solution of diluted hand-soap liquid initially. If this does not produce a satisfactory result,
repeatthecleaningusingasolvent(OpticalCleaningSolution85%petroleumetherand15%isopropanol).
6. Greasemustalwaysberemovedusingasolvent.
7. Cleaning is achieved by using a spiral motion from the center to the rim. Never wipe using zig-zag movements as
thiswillonlyspreadthedirt.Withlargeropticalsurfaces(e.g.tubelenses)thespiralmotionstartsinitiallyattherim
beforemovingto themiddle andisthen followedbya centertorim cleaningmotion.Normally severalspiralwipes
arerecommended.
Werecommendpure,volatilepetroleumetherorOpticalCleaningSolutionasexplainedinpoint3above.
Wipeusingaspiralmovement.Donotuseazig-zagmotion!
2. Cleaning of painted surfaces :
Avoid the use of any organic solvent ( e.g. thinner, xylene, ether, alcohol etc.) for cleaning of painted surfaces of
the instrument. Painted surfaces can be cleaned with a very lightly moistened micro fiber cloth. Loose dust and
otherdirtparticlescanberemovedusingasoftbristlebrushusedexclusivelyforthispurpose.
zig-zag motion (X) spiral motion ( )
4
Lx 500

5
Lx 500 Binocular
3Lx 500
Eyepieces
Revolving nosepiece
Objectives
Specimen holder
Aperture iris
diaphragm ring
Abbe condensor
Filter holder
X-Y movement control knob
Coarse and
fine focus knob
Mechanical
stage
Observation tube
clamping screw
Binocular viewing tube, 30° inclined

6
Lx 500 Trinocular
4Lx 500
Eyepieces
Revolving nosepiece
Objectives
Specimen holder
Aperture iris
diaphragm ring
Abbe condensor
Filter holder
Trinocular viewing tube, 30° inclined
Trinocular Port
Observation tube
clamping screw
Mechanical
stage
Coarse and
fine focus knob
X-Y movement control knob

7
Lx 500
Lx 500 Ergonomic Binocular
5
Eyepieces
Revolving nosepiece
Objectives
Specimen holder
Aperture iris
diaphragm ring
Abbe condensor
Filter holder
Observation tube
clamping screw
Mechanical
stage
Coarse and
fine focus knob
Ergonomic Binocular viewing tube,
0° to 25° inclined
X-Y movement control knob

8
Daylight (blue) filter
Power Cord
Allen Wrench 3mm
Paired Eyepieces
¡After removing your microscope from its packaging, make sure that all of the following contents are present.
“Please note that the contents of your microscope may vary as the optional configuration, contrasting method or
viewing bodyoptedformaynotbeofthestandardconfigurationhighlightedhere”
STANDARD COMPONENTS
6Lx 500
Eyepieces
Revolving nosepiece
Objectives
Specimen holder
Aperture iris
diaphragm ring
Abbe condensor
Filter holder Coarse and
fine focus knob
Mechanical
stage
Observation tube
clamping screw
Binocular viewing tube, 30° inclined

9
System Diagram of Optional Accessories
Installation and Operation of Optional Accessories
1.Mount the Video adapter 1/2” (part # 3143300-912) on Trinocular
observation head.
2. Mount iVu Camera Module System on video adapter.
1iVu Camera Module System
Fig. 4
OPTIONAL ACCESSORIES
7
Flip top condenser
Trinocular head
Polarizer Mount Analyzer Mount
WF 10x WF 20x
Video adapter1/2"&1/3" Digital camera adapter
Binocular head WF 16xErgonomic head
Blue filter Green filter
iVu 1500 iVu 3000 iVu 5000 iVu 7000
Halogen bulb
RP 4x RP 10x RP 20x RP 40x (SL) RP 50x (SL, Oil) RP 100x (SL, Oil) RP PH 10x RP PH 20x RP PH 40x (SL) RP PH 100x (SL, Oil)
RP FL 4x RP FL 10x RP FL20x RP FL 40x (SL) RP FL 50x (SL, Oil) RP FL 100x (SL, Oil) RP 100x,(Oil, Iris)

1. Remove the condenser from its position by loosening the two thumb
screws(1)onbothsidesofthecondenserasshowninfigure6.
2. Place the phase annulus turret in position from where the condenser
hasbeenremoved.
3. The long “push in to engage” thumbscrews (2) on both sides of the
phase turret are provided for proper alignment of the phase ring. By
disengaging these screws, the phase turret can be adjusted at any
desired phase annulus (10x, 20x, 40x & 100x) by turning the
thumbwheel to the desired position. There is also an ‘0’setting on the
thumbwheelforbrightfieldapplication
Note: Refer to the user manual provided with the Phase Contrast
kit for centering and alignment.
10Xeyepiecesareprovided.Toreplace:
1.Pulloutthe10xeyepiecesout fromtheobservationheadsoculartube.
2.Insertdesired eyepiecesinemptyoculartube.
3
2
Phase Turret
Optional Eyepieces
Fig. 5
Fig. 6
10
Lx 500
1
2

INITIAL SETUP
11
8
1
2
3
Objectives
Observation Head
Eyepieces
Objectives are factory set. Objectives are par-centered and par-
focalised during assembly.
All objectives have been secured for a tight fit to prevent them from
coming loose during transit. To remove an objective, rotate it
counterclockwise while holding it with a rubber grip to avoid any
slippage.
Installtheobservationheadusingthefollowingprocedure:
1. Using allen wrench 3mm (provided), loosen the Head Locking
Screw (1) and remove the dust cover cap provided in dovetail
cavityaswellasonobservationheaddovetail.
2. Mount the Observation Head by engaging the dovetail provided
at the bottom of the head into the dovetail cavity provided in the
microscopesarm.
3. Tighten the Head Locking Screw (1) after positioning the
ObservationHeadasdesired.Seefigure8.
Fig. 7
Fig. 8
Fig.9
1
Insert the eyepieces into the ocular tube of Observation Head using
followingprocedure:
1. Removetheprotectivecapsfromtheobservationtube.
2. Insert 10x eyepieces into the spring loaded and to prevent
undesiredrotationofeyepieces. Seefigure9.

12
This filter modifies the color of observation light into a natural (daylight
color).
• Fit the filter (1) into the bottom of the condenser (2) until it clicks into
place. (Figure 11).
1
2
Installing or Replacing the Lamp Bulb
Mounting the Daylight (Blue) Filter
Each standard set can be assembled by simply attaching the bulb, filter and power cord.
Fig. 10
ASSEMBLY
9Lx 500
Beforeattachingthe lampbulb,remove the partsthatmay dropsuchas the
filter and specimen from the microscope frame, and place the microscope
onitsbacksothatthebottomplateisexposed.
1.Pulloutthelamphousecover(1) todetachit.
2.Pullupthelampfromthelampholder&replacewithlamp.
3.Replacethelamphousecover.
Always use the designated bulb. Using a bulb other than those
specified by LABOMED may lead to a fire hazard and decentering of
light. Fingerprints or stains on the lamp bulb reduce its life. If
contamination occurs, wipe bulb surface with a cloth slightly
moistened with alcohol.
Caution: For Bulb Replacement During Use or Right After Use
The bulb, lamp socket and areas near these will be extremely hot during and right after use. Set the main
switch to" O" (OFF), disconnect the power cord from the wall outlet, and allow the bulb and lamp socket to
cool before replacing the bulb with a new bulb of the designated type. Cooling time may vary to users
discretion.
Fig. 11
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2
1

Flip the main switch to “ON”
Place the specimen on the stage.
Engage the 10X objective in the light path.
Bring the specimen in focus.
Adjust the aperture iris diaphragm.
Engage the objective to be used in the light
path and bring the specimen in focus.
Adjust the observation tube and
Adjust the interpupillary distance.
Adjust the dioptric setting.
Engage the required filters.
Adjust the brightness.
Observe Specimen.
A/C Inlet
On/Off
SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE
13
10
eyepieces

1. Flip ” ” asshowninFigure12.
2. Rotating the light intensity adjustment knob (Fig. 13) in the direction
of the arrow increases brightness and rotating knob in the opposite
direction decreases brightness. The intensity bar next to the knob
indicates the direction of intensity level.
themainswitch to I(ON)
1
2
Turning the Lamp ON
Placing specimen on the stage
Fig. 13
Fig. 14
Fig. 12
2
3
2
4
5
1
DETAILED OBSERVATION PROCEDURE
11 Lx 500
1. Rotatethecoarseadjustmentknob(2)inanticlockwisedirectiontofully
lowerthestage.
2. Open the bow-shaped lever (3) outward by pulling on lever handle (1),
placethe specimenbysliding thespecimen glassplate(s)onthestage
fromthefronttowardtheslideseatatthe rear.
3. After positioning your specimen slides,(2 max) return the bow-shaped
lever(3)gentlybyslowlyreleasingcontrolknob(1).
4. Rotating the upper co-axial knob controlling the Y-axis movement (4)
moves the specimen in the vertical direction. Rotating the lower knob
(5) movesthespecimenintheX-axisor horizontaldirection.
Do not adjust the specimen holder directly by hand. This will
damage the rotary mechanisms.
When the specimen holder reaches stop position, the rotation
force of the X/Y knobs become stiff. Stop rotating the at this point.
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1
14

“Vernier Scales”
These scales allow for easy identification of the specimen’s position
(coordinates), making it easy to return to a particular region of interest
after scanning the slide. (Figure 16.)
1. The horizontal coordinate can be read at position (1) on the specimen
holder.
2. The vertical coordinate can be read at the index line (2).
Focusing Procedure
1.Rotatethecoarseadjustmentknob(1)clockwisesothattheobjective
(3)isascloseaspossibletothespecimen(Werecommendstartingwith
10X).
2.Whileobservingthespecimenthroughtheeyepieces,slowlyrotatethe
coarseadjustmentknob(1) counterclockwisetolowerthestage.
3.Whencoarsefocusingofthespecimenisobtained(ablurrimageis
observed), rotate the fine adjustment knob (2) for fine detail focusing in
anticlockwisedirection.
Working Distance (WD)
The WD refers to the distance between each objective and the specimen,
3Adjusting the Focus
Objective
Magnification 4X 10X 40X 100X
WD (mm) 30.5 4.82 0.55 0.11
Cover glass
This is the glass plate placed on the specimen. For optimum optical
performance, the cover glass thickness, which is the distance from its
surface to the specimen surface, should be 0.17 mm.
Slide glass
This glass plate should ideally have a length of 76 mm, width of 26 mm
±1 mm and thickness between 0.9 and 1.4 mm.
Cover glass
Slide glass
Fig. 15
Fig. 16
Fig. 17
1
2
1
2
3
WD
15

The inter-pupillary distance adjustment consists of moving the two
eyepieces to align with both eyes’ pupils so that you can observe a
single microscopic image through two eyepieces in stereo vision. This
greatly helps to reduce fatigue and discomfort during observation.
While looking through the eyepieces, move both eyepieces
laterally until the left and right fields of view coincide completely. The
position of index dot (•) indicates the inter-pupiliary distance value.
Note your interpupillary distance so that it can be quickly referred to in
the future. This may need when multiple users work with the
microscope.
4
5
Adjusting the Interpupillary Distance (IPD)
Adjusting the Diopter
Using the Eye Guards
When Wearing Eyeglasses
Use with the eye guards in the normal, folded-down position. This will
prevent the eyeglasses from being scratched.
When Not Wearing Eyeglasses
Extend the folded eye guards outwards (direction of the arrow) to
prevent ambient light from entering into your line of vision.
Fig. 18
Fig. 19
Fig. 20
16
Lx 500
Procedure for adjusting the diopter:
1. Rotate the right eyepiece to match the markings of your IPD (If your
IPD is 64, rotate the eyepiece to 64 mark).
2. While looking through the right eyepiece with your right eye, rotate
the coarse and fine adjustment knobs to bring the specimen into
focus.
3. While looking through the left eyepiece with your left eye, rotate only
diopter adjustment ring on the eyepiece until specimen is at its best
possible focus.

The condenser is most often used in the highest position. If the observed
field of view is not bright enough, brightness may be improved by lowering
thecondenserslightly
1.Rotatethecondenserheightadjustmentknob(2)tomovethe
condensertothehighestordesiredposition.
2.Theapertureirisdiaphragmring(1)hasanobjectivemagnificationscale
(4X,10X,40X,100X).Rotatetheringsothatthemagnificationofthe
objectiveisclearlyvisiblewhileusingthemicroscope.
Rotatetherevolvingnosepiece(1)sothattheobjectivetobeusedisinline
above the specimen. Always use the ribbed grip(1) to rotate the objective
nosepiece.
6
7
Adjusting the Condenser Position and Aperture Iris Diaphragm
Switching the Objectives
Fig. 22
Fig. 23
17
1
Thereisapossibilityofhavingthecondenserde-centeredduringshipping.
it is mandatory to check the centering before observing the specimen as it
isrequiredtodistributeevenlighttothespecimen.
For models with Koehler illumination:
1. Bringthe10xobjective(1)inthefrontposition.
2. Movethecondensertothehighestpoint.
3. Close the koehler. (2) Focus the light spot as show in the illustration
below.
4. Use the centering screws (3) provided on left and right sides of the
condenserbrackettobringthelightspottocenteroftheviewfield.
6Centering the Condenser
Fig. 21
1
2