Beckman Coulter Allegra 21 Series User manual

GS15-IM-8
Allegra 21 Series, GS-15 Series,
and Spinchron 15 Series Centrifuges
Instruction Manual
IMBALANCE
ENTER
PULSE
ACCEL
DECEL
TEMP
RPM RCF
ROTOR
TIME
STOP
START
FAST
STOP
OPEN
DOOR
SPEED
TIME TEMP °C ACC/DEC
®

Symbol
Symbol
Symbole
Símbolo
Title / Titel / Titre / Titulo / Titolo /
Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione
Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate
On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)
Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)
Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra
Earth (ground)
Erde
Terre
Tierra
Scarica a terra
!
/
Simbolo

SAFETY NOTICE
This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions shouldbe read and understood before installation, operation,maintenance,
or repair of this instrument. When you see the symbol on other pages, pay special attention
to the safety information presented. Observance of safety precautions will also help to avoid
actions that could damage or adversely affect the performance of the instrument.
Other symbols may also be displayed on the equipment. These are reproduced and described
on the reverse side of the cover page.
Safety During Installation and/or Maintenance
These centrifuges each weigh 50 kg/110.3 lb (nonrefrigerated model) or 70 kg/154.4 lb
(refrigerated model). DO NOT attempt to lift or move one of them without assistance from
another person.
Be sure to use the anchoring system to secure the centrifuge in place. The anchoring system
is designed to reduce the possibility of injury or damage which could result from instrument
movement in the event of a major rotor mishap.
Any servicing of this equipment that requires removal of any covers can expose parts which
involve the risk of electric shock or personal injury. Make sure that the power switch is turned
off and the instrument is disconnected from the main power source, and refer such servicing
to qualified personnel.
Do not replace any centrifuge components with parts not specified for use on this instrument.
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and
plug to connect this equipment to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed
to the instrument.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may
get into the instrument and damage electrical or mechanical components.
Safety Against Risk of Fire
Certain electrical circuits within this equipment are protected by fuses against overcurrent
conditions. For continued protection against the risk of fire, replace only with the same type
and rating specified.
This centrifuge is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (chloroform or ethyl alcohol for example)
!

in this instrument nor handle or store them within the 30-cm (1-ft) clearance envelope surrounding
the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this centrifuge.
• Before starting the centrifuge, make sure that the rotor tie-down screw is securely fastened.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop the rotor by hand.
• Do not lift or move the centrifuge while the rotor is turning.
• If a glass tube breaks inside the chamber bowl, be careful when examining or cleaning the
gasket or chamber, as sharp glass fragments may be embedded in their surfaces.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge (secured with antirotation
kit) while it is running. During operation you should come within the envelope only to adjust
instrument controls, if necessary. Never bring any flammable substances within the 30-cm (1-ft)
area surrounding the centrifuge. Never lean on the centrifuge or place items on the centrifuge
while it is operating.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument, however, unless all necessary
safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to their use.
• Because spills may generate aerosols, observe proper safety precautions for aerosol
containment.
• Handle all infectious samples according to good laboratory practices and methods to prevent
the spread of disease. Ask your laboratory safety officer to advise you about the level of
containment required for your application and about the proper decontamination or sterilization
procedures to follow if fluids escape from containers. Biosafe containment should be used when
Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety
Manual) are handled; materials of a higher group require more than one level of protection.
Because spills may generate aerosols, observe proper safety precautions for aerosol
containment.
• Other infectious samples must also be handled according to good laboratory procedures and
methods to prevent spread of disease.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before requesting
service by our Field Service representative.

GS15-IM-8
April 2009
Allegra 21 Series, GS-15 Series,
and Spinchron 15 Series Centrifuges
Instruction Manual
IMBALANCE
ENTER
PULSE
ACCEL
DECEL
TEMP
RPMRCF
ROTOR
TIME
STOP
START
FAST
STOP
OPEN
DOOR
SPEED
TIMETEMP°C ACC/DEC
© 2009 Beckman Coulter, Inc.

Contents
iii
Page
INTRODUCTION
Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Scope of Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
CFC-Free Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Nonrefrigerated Model Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . xi
Refrigerated Model Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
SECTION 1: DESCRIPTION
Instrument Function and Safety Features. . . . . . . . . . . . . . . . . . . . . . . 1-1
Instrument Function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Safety Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Door. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Temperature Sensing and Control. . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Power Switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Control Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
SECTION 2: INSTALLATION
Installing the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Test Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

iv
Contents
Page
SECTION 3: OPERATION
Run Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Preparation and Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Entering Run Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Changing Parameters During a Run . . . . . . . . . . . . . . . . . . . . . . 3-11
Stopping a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Unloading. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Summary of Run Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13
SECTION 4: TROUBLESHOOTING
User Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Other Possible Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Emergency Access . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
SECTION 5: CARE AND MAINTENANCE
Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Preventive Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Replacing the Rotor Chamber Gasket . . . . . . . . . . . . . . . . . . . . . . .5-2
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Tube Breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Sterilization and Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Storage and Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Returning a Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
APPENDIX: PROGRAM LIBRARY

Illustrations
v
Tables
Page
Figure 1-1. The Allegra 21 and Allegra 21R Centrifuges. . . . . . . . . . . . . . . . . . . . . 1-2
Figure 1-2. Interior View of the Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Figure 1-3. The Power Switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Figure 1-4. The Control Panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Figure 2-1. Dimensions of the Nonrefrigerated and Refrigerated
Centrifuges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Figure 3-1. Tapered Sleeve Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Figure 3-2. Installing a Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Figure 3-3. Default Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Figure 4-1. Emergency Door Release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Figure 5-1. Replacing the Rotor Chamber Gasket . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Page
Table 3-1. Acceleration/Deceleration Times (in minutes:seconds) . . . . . . . . . . . . . 3-9
Table 4-1. Error Message Chart. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Table 4-2. Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

Introduction
vii
CERTIFICATION
To ensure full system quality, Beckman Coulter Allegra 21 series, GS-15
series, and Spinchron 15 series centrifuges are manufactured in a registered
ISO 9001 or 13485 facility. They have been designed and tested to be
compliant (when used with Beckman rotors) with the laboratory equipment
requirements of applicable regulatory agencies. Declarations of conformity
and certificates of compliance are available at www.beckmancoulter.com.
International symbols that may be displayed on the centrifuge are illustrated
and described on the reverse side of the cover page.
SCOPE OF MANUAL
This manual is designed to familiarize you with the Beckman Coulter
Allegra 21 series, GS-15 series, and Spinchron 15 series benchtop centri-
fuges, their functions, specifications, operation, and routine operator care
and maintenance.
• The following introductory pages contain the instrument specifications,
as well as space, electrical, and temperature conditions required for
optimal centrifuge performance. A list of available rotors is also
included.
• Section 1 provides a brief physical and functional description of the
centrifuge and the operating controls and indicators.
• Section 2 contains instructions for installing and connecting the
centrifuge.
• Procedures for operating the centrifuge are summarized in Section 3.

Introduction
viii
• Section 4 lists possible error messages and/or malfunctions, together
with probable causes and corrective actions required.
• Procedures for operator care and maintenance are presented in
Section 5, as well as a brief list of supplies and replacement parts.
• A Program Library chart is provided at the back of this manual. You
can use this chart to record the parameters of a run for later duplication
of the run conditions.
We recommend that you read this entire manual, especially the SAFETY
NOTICE and all safety-related information, before operating the centrifuge
or performing instrument maintenance.
➠NOTE
If the centrifuge is used in a manner other than spec-
ified in this manual, the safety and performance of
this equipment could be impaired. Further, the use of
any equipment other than that intended for use by
Beckman Coulter has not been evaluated for safety.
Use of any equipment not specifically recommended
in this manual is the sole responsibility of the user.
CONVENTIONS
NOTES, CAUTIONS,
AND WARNINGS Certain symbols are used in the manual to call out safety related and other
important information. These are reproduced and described below and/or
on the inside of the front cover.
➠NOTE
Used to call attention to information that should be
followed during installation, use, and/or servicing of
the equipment.

Introduction
ix
!CAUTION
Used to indicate a potential hazardous situation
which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It is
also used to alert against unsafe practices.
WARNING
!Used whenever an action or condition may poten-
tially cause serious personal injury or loss of life.
Mechanical damage may also result.
WARNING
!
or Indicates high voltage or risk of electric shock.
Turn the power switch off and disconnect the
equipment from the main power source. Refer
servicing of all areas displaying either symbol to
a service personnel.
TYPOGRAPHIC
CONVENTIONS Certain typographic conventions are used throughout this manual to
distinguish names of user interface components, such as keysand displays.
•Key names (for example, [START] or [ENTER]) appear in brackets.
•Display names (for example, TEMP°C or SPEED) appear in bold
type.
•Cursor keys, used to increment values up or down when setting
parameters, are shown as up and down arrows (▲or ▼).

Introduction
x
CFC-FREE CENTRIFUGATION
To ensure minimal environmental impact, no CFCs are used in the
manufacture or operation of Allegra 21, GS-15, or Spinchron 15 series
centrifuges.
RADIO INTERFERENCE
This instrument has been tested and found to comply with the limits for a
Class A digital device, pursuant to Part 15 of FCC Rules. These limits are
designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial environment. This equip-
ment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with this instruction manual, may cause
interference to radio communications. Operation of this equipment in a
residential area may cause interference, in which case the user will be
required to correct the interference at his or her own expense.
CANADIAN
REGULATIONS This digital apparatus does not exceed the Class A limits for radio noise
emissions from digital apparatus as set out in the radio interference regu-
lations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques
dépassant les limites applicables aux appareils numériques de Classe A
prescrites dans le reglement sur le brouillage radioelectrique édicté par le
Ministère des Communications du Canada.
RECYCLING LABEL
This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates:
1) the device was put on the European market after August 13, 2005 and
2) the device is not to be disposed via the municipal waste collection
system of any member state of the European Union.
It is very important that customers understand and follow all laws regard-
ing the proper decontamination and safe disposal of electrical equipment.
For Beckman Coulter products bearing this label please contact your
dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery,
recycling and safe disposal of the device.
CFC
A28219-AA
Note: On the instrument, the
triangle background is yellow
rather than gray.

Introduction
xi
NONREFRIGERATED MODEL SPECIFICATIONS
Only values with tolerances or limits are guaranteed data. Values without
tolerances are informative data, without guarantee.
Speed
Set speed. . . . . . . . . . . . . . . . . . . . . 0 to 14 500 rpm (in 100-rpm increments)
or equivalent RCF
Time
Set time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . to 9 hr 59 min or continuous
Time display. . . . . . . . . . . . . . . . . . . . . . . . time remaining in run (timed run)
or ∞and elapsed time (continuous run)
Acceleration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 acceleration profiles
Deceleration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 deceleration profiles
Ambient temperature range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10to35°C
Humidity restrictions . . . . . . . . . . . . . . . . . . . . . . . . . . . <95% (noncondensing)
Dimensions
Width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 cm (16.6 in.)
Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 cm (19.3 in.)
Height, door closed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 cm (15.0 in.)
Height, door open. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 cm (31.5 in.)
Weight. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 kg (110.3 lb)
Clearances (sides and rear) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.6 cm (3.0 in.)
Electrical requirements. . . . . . . . . 50 Hz, 230 VAC, 3 A; 60 Hz, 120 VAC, 5 A;
50/60 Hz, 100 VAC, 15 A
Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 340 W
Electrical supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Class I
Maximum heat dissipation into room
under steady-state conditions . . . . . . . . . . . . . . . . . . . . 1160 Btu/h (0.34 kW)
Noise level 0.91 m (3 ft) in front of instrument . . . . . . . . . . . . . . . . . . . ≤64 dBa
Installation (overvoltage) category. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . II
Pollution degree . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2*
_______________
*Normally only nonconductive pollution occurs, occasionally, however, a temporary conductivity caused by condensation must be expected.

Introduction
xii
REFRIGERATED MODEL SPECIFICATIONS
Only values with tolerances or limits are guaranteed data. Values without
tolerances are informative data, without guarantee.
Speed
Set speed. . . . . . . . . . . . . . . . . . . . . 0 to 15 300 rpm (in 100-rpm increments)
or equivalent RCF
Time
Set time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . to 9 hr 59 min or continuous
Time display. . . . . . . . . . . . . . . . . . . . . . . . time remaining in run (timed run)
or ∞and elapsed time (continuous run)
Temperature
Temperature setting . . . . . . . . . . . . . . . . . . –20 to +40°C (in 1°C increments)
Operating range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 to 40°CF*
Ambient temperature range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 to 30°C
Humidity restrictions . . . . . . . . . . . . . . . . . . . . . . . . . . . <95% (noncondensing)
Acceleration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 acceleration profiles
Deceleration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 deceleration profiles
Dimensions
Width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 cm (16.6 in.)
Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 cm (25.2 in.)
Height, door closed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 cm (15.0 in.)
Height, door open. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 cm (31.5 in.)
Weight. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 kg (154.4 lb)
Clearances (sides and rear) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7.6 cm (3.0 in.)
Electrical requirements. . . . . . . . 50 Hz, 230 VAC, 5 A; 60 Hz, 120 VAC, 12 A;
50/60 Hz, 100 VAC, 15 A
Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 746 W
Electrical supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Class I
Maximum heat dissipation into room
under steady-state conditions . . . . . . . . . . . . . . . . . . . . 2546 Btu/h (0.75 kW)
Noise level 0.91 m (3 ft) in front of instrument . . . . . . . . . . . . . . . . . . . ≤64 dBa
Installation (overvoltage) category. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . II
Pollution degree . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2†
*Temperature range depends on rotor in use and speed (see applicable rotor manual).
†Normally only nonconductive pollution occurs, occasionally, however, a temporary conductivity caused by condensation must be expected.

Introduction
xiii
AVAILABLE ROTORS
*Values are rounded off.
Rotor Profile and
Description
Refrigerated Nonrefrigerated
Max
Capacity
(mL) Rotor Part Number/ Rotor Manual Number
Max
RPM
Max
RCF*
(×g)Max RPM Max RCF* (× g)
F2402H
Fixed Angle
45° Angle
15 300 21 460 14 500 19 280 24 ×1.0/1.5 361171
GS-TB-021
F3602
Fixed Angle
45° Angle
14 000 19 280 12 500 15 370 36 ×2.0/1.5 364600
GS-TB-006
F0630
Fixed Angle
30° Angle
15 300 20 410 14 500 18 340 6 ×30 361231
GS-TB-014
F0850
Fixed Angle
25° Angle
10 000 10 730 9 000 8 690 8 ×50 346640
GS-TB-003
F0685
Fixed Angle
25° Angle
8 000 7 010 8 000 7 010 6 ×85 364650
GS-TB-008
F1010
Fixed Angle
35° Angle
15 300 19 890 14 500 17 870 10 ×10 361221
GS-TB-007
C0650 (Conical)
Fixed Angle
25° Angle
9 500 9 380 9 000 8 420 6 ×50 364670
GS-TB-009
C1015 (Conical)
Fixed Angle
25° Angle
9 500 9 380 9 000 8 420 10 ×15 364680
GS-TB-011
S4180
Swinging Bucket
Four buckets
5 500 5 450 4 500 3 650 4 ×180 361109
GS-TB-004
S2096
Microtiter 3 000 1 100 3 000 1 100 96 ×1.5/1.8 361111 GS-TB-005
H6002
BioSafe
Bowl Rotor
12 200 12 400 12 200 12 400 60 ×1.5/1.8
72 ×600 μL
84 ×250 μL
363000
GS30-TB-002

1
Description
1-1
This section provides a brief physical and functional description of the
Beckman Coulter Allegra 21 series, GS-15 series, and Spinchron 15
series centrifuges. The operating controls and indicators are also
described; instructions for their use are in Section 3. Chemical compati-
bilities of materials listed in this manual can be found in Chemical
Resistances (publication IN-175). Refer to the applicable rotor manuals
for rotor descriptions.
INSTRUMENT FUNCTION AND SAFETY FEATURES
INSTRUMENT FUNCTION The Beckman Coulter Allegra 21 series, GS-15 series, and Spinchron 15
series compact benchtop centrifuges generate centrifugal forces required
for a wide variety of applications. Together with any of several Beckman
Coulter rotors designed specifically for use in these centrifuges, the instru-
ment applications include:
• Routine processing such as sample preparations, pelleting, extractions,
purifications, concentrations, phase separations, receptor binding, and
column centrifugations.
• Processing large numbers of small-volume samples in multiwell plates
for concentrating tissue-culture cells, cloning and replicate studies,
in-vitro cytoxicity studies, receptor binding, and genetic engineering
experimentation.
• Rapid sedimentation of protein precipitates, large particles, and cell
debris.
• Binding studies and separation of whole blood.
• Cell isolation.

Description
1-2
The centrifuges are microprocessor-controlled, providing interactive oper-
ation.The instrumentdesign featuresa brushlessthree-phasedrive system,
automatic rotor overspeed identification system, program memory that
can repeat the previously used run parameters for a given rotor, and
a choice of acceleration/deceleration rates. The refrigerated models also
have temperature control systems. User messages alert the operator to
conditions that may need attention.
MODELS The centrifuge is available in both nonrefrigerated and refrigerated
models. See Figure 1-1. See the SPECIFICATIONS listed above for
the operating differences between the refrigerated and nonrefrigerated
models. Unless indicated otherwise, information in this manual is the
same for both models.
Figure 1-1. The Allegra 21 and Allegra 21R Centrifuges. GS-15, GS-15R,
Spinchron 15, and Spinchron 15R models are similar in appearance.