Biobase BBS-V800 User manual

Vertical Laminar Flow Cabinet
BBS-V800
User Manual
BIOBASE GROUP
Version 2021.12

1
Preface
Thank you very much for purchasing our vertical laminar flow cabinet.
Please read the “Operating Instructions” and “Warranty” before operating this unit to assure proper
operation. After reading these documents, be sure to store them securely together with the
“Warranty” within reach for future reference.
Warning: Before operating the unit, be sure to read carefully and fully understand important
warnings in the operating instructions.

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Content
Preface.................................................................................................................................................... 1
Content................................................................................................................................................... 2
1.Unpacking, Installation and Debugging..............................................................................................4
1.1 Unpacking................................................................................................................................. 4
1.2 Accessories Checking................................................................................................................6
1.3 Installation Conditions and Operating Environment.................................................................6
1.4 Installation (Picture 4)............................................................................................................... 6
1.5 Checking after Installation........................................................................................................ 7
2. User Instructions.................................................................................................................................8
2.1 Functions................................................................................................................................... 8
2.1.1 Product Concept.............................................................................................................. 8
2.1.2 Working theory/Air flow Pattern and Protected Area Figure (Picture 5)....................... 8
2.1.3 Protected Objects.............................................................................................................8
2.1.4 Technical Parameters...................................................................................................... 8
2.2 Product Structure.......................................................................................................................9
2.2.1 Structural Composition of BBS-V800 (Picture 6).......................................................... 9
2.2.2 Structure Composition...................................................................................................10
2.3 Instructions for Operation....................................................................................................... 11
2.3.1 Normal Operation Notice.............................................................................................. 11
2.3.2 Operation Process ........................................................................................................12
2.4 Daily Maintenance.................................................................................................................. 12
2.4.1 Clean the Surface of Working Zone..............................................................................12
2.4.2 Clean the External Surface and Front Window.............................................................12
2.4.3 Overall Maintenance Period..........................................................................................13
2.4.4 Maintenance Methods................................................................................................... 13
2.4.5 Storage Conditions........................................................................................................ 13
2.5 Replacement Parts List............................................................................................................13
2.6 Wiring Diagram (Picture 8).....................................................................................................15

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3. Trouble Shooting and Solutions.......................................................................................................16
3.1 Common Failures and Solutions............................................................................................. 16
3.2 Replacement of Fuse............................................................................................................... 17
3.3 Replacement of LED Lamp.....................................................................................................17
3.4 Replacement of UV Lamp.......................................................................................................18
3.5 Label Description.................................................................................................................... 19
4. Warranty...........................................................................................................................................19

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1.Unpacking, Installation and Debugging
Please firstly check if the packing box is in good condition. If the packing box is damaged, please
take photos.
1.1 Unpacking
Choose the proper unpacking method and tools as shown in the below picture
For wooden box:
1) Method 1 Necessary tools for unpacking: Electric drill with hexagon dead M8 (Picture 1).
Picture 1

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2) Method 2 Use M8 Wrench to unpack (Picture 2).
Picture 2
The following diagram demonstrates quick unpacking procedures (Picture 3). Remove the screws as
shown in the below diagram, then remove the wooden sheet to right and wooden box to left.
Picture 3

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1.2 Accessories Checking
Refer to the packing list and check the accessories.
The spare parts as back up, it can be normally used after unpacking.
Packing list (BBS-V800)
Items
Quantity
Position
Main body
1 unit
Wooden box
Base stand
1 set
Connected with cabinet body
Power cord
1 pc
Packaging Bag（On top of main body）
Fuse (5A)
1 pc
Packaging Bag（On top of main body）
UV Lamp（T6 20W）
1 pc
Paper Packing（On top of main body）
User manual
1 pc
File Packet（On top of main body）
Inspection report
1 pc
File Packet（On top of main body）
Certification of quality
1 pc
File Packet（On top of main body）
Warranty Card
1 pc
File Packet（On top of main body）
1.3 Installation Conditions and Operating Environment
Laminar Flow Cabinet shall be placed in the protective area of an air stream, and the working area of
the Laminar Flow Cabinet cannot be right opposite to the door or window, and should be away from
the air outlet of air conditioner to prevent the air flow from the ventilation system, air conditioning,
door, window and personnel movement may cause airflow impact on the LAF.
At least 300mm gap must be kept in each side of the Laminar Air Flow for inspection.
Working environment:
(1) Only applicable for indoor operation
(2) Ambient temperature: 15° C～35° C
(3) Relative Humidity: ≤75%;
(4) Atmospheric pressure range: 70 kPa～106 kPa;
(5) Electrical parameters: Adequate power supply to the laminar flow cabinet (See 2.1.4 Technical
Parameters);
(6) Power supply need to be grounded; (Judging method: Test the live wire and the neutral wire of
the socket with multimeter. The voltage between live and ground should equal to the voltage of local
electrical grid, and the voltage between neutral and ground should equal to 0. Otherwise the power
supply is not grounded correctly).
1.4 Installation (Picture 4)
a. Remove all the packing materials;
b. Check for Physical damage or scratch the surface of main body during transit. Make sure there is
no damage, scratch, deformation on main body;
c. Confirm the complement of accessories according to the list;
d. The height of leveling feet can be adjusted. Counterclockwise the leveling feet, cabinet (or base
stand)can be moved when the height of leveling feet is less than the height of caster; clockwise the
leveling feet, cabinet (or base stand)can be moved when the height of leveling feet is larger than the
height of caster;
e. The whole LAF Instrument must be moved to the final working place where it can be Installed.

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Picture 4
1.5 Checking after Installation
First, check the electrical power supply, make sure the voltage and frequency match with the
Instrument power supply rating, then check the follows items after power on：
Checking Items
Normal working status
Fan
Runs normally
LED Lamp
Lamp lights up after pressing button
UV Lamp
Lamp lights up after pressing button
Screen Buttons
All buttons work effectively
If you have any questions, please contact BIOBASE or our agent to debug. Debugging
method in the After-sale service manual.

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2. User Instructions
2.1 Functions
2.1.1 Product Concept
Laminar Flow Cabinet –LAF Cabinet is only for sample protection.
Laminar Flow Cabinet is a work bench or similar enclosure, which creates a particles-free working
environment by taking air though a filtration system and exhausting it across a work surface in a
laminar or unidirectional air stream. The laminar flow cabinet is enclosed on the sides and kept under
constant positive pressure in order to prevent the infiltration of contaminated room air. BBS-V800 is
vertical type Laminar Flow Cabinet.
2.1.2 Working theory/Air flow Pattern and Protected Area Figure (Picture 5)
Picture 5
2.1.3 Protected Objects
Laminar flow cabinets are designed to protect samples and create partial particles-free environment.
The main function is to make sure the accuracy of experiment at the operating area. However, it
could not protect the environment or operators.
2.1.4 Technical Parameters
Model
Parameters
BBS-V800
External Size
(W*D*H)
802*650*1550 mm
Internal Size
(W*D*H)
800*530*540 mm
Power Supply AC
220V±10% 110V±10％
Frequency
50Hz 60Hz

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Consumption
≤350 W
Airflow Velocity
0.3～0.5m/s
UV Lamp
Consumption
20W
LED Lamp
Consumption
8W (LED)
HEPA Filter
Efficiency
99.999％efficiency at 0.3um
Noise
<65dB
Note: the company reserves all the rights to changes in product design , if there are any
design change, we will not inform in advance.
Vibration Amplitude:
The net vibration amplitude between frequency 10Hz and 10KHz should not exceed 5μm(rms).
1) Illumination
The average illumination is equal or more than 350 lux,
2) Electrical properties
The voltage increases to 1390V (AC) in 5s and keep for another 5s without breakdown.
Grounding resistance ≤0.1Ω.
2.2 Product Structure
2.2.1 Structural Composition of BBS-V800 (Picture 6)
Picture 6
1. Socket
7. Handle
2. Control panel
8. Work table
3. LED light
9. Base stand

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4. UV lamp
10. Caster
5. Front window
11. Leveling feet
6. Rear window
2.2.2 Structure Composition
1）Driving system of front window
Driving system consists of constant spring and front window.
2) Air filtration system
Air filtration system is the core system of laminar flow cabinet. It consists of blower and HEPA filter
system. The main function of air filtration system is to transfer filtered air to work area, ensure the
airflow velocity, and keep cleanness of work area.
3) UV lamp
The UV lamp located at the top of the work zone effectively disinfects the entire work zone by
emitting a wavelength of 253.7nm
4) LED light
The laminar flow cabinet is equipped with LED light, which ensures the standard requirement of
average illumination is met.
5) Control panel (Picture 7)
Picture 7
1.Gears Indicator
6.UV Indicator
2.Increase Air Velocity
7.UV
3.Decrease Air Velocity
8.Light Indicator
4.Fans Indicator
9.Light
5.Fans
a. Gear indicator
To see air speed gears of the equipment through gears indicator.
b. Soft touch buttons
Laminar flow cabinet’s main functions are executed by touch-buttons.
：To control LED lamp.

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：To control UV lamp(UV is interlocked with blower and LED light,
which means that the UV lamp will auto off when blower or LED light is opened)
：To control blower working status.
Air velocity control( )：During working, press to increase air velocity; press to
decrease air velocity.
6) Fuse
The cabinet is equipped with main power fuse. Fuse label is corresponding to the relevant
specifications, please refer to 3.5.1 Fuse Label. And refer to 3.2 Replacement of Fuse when replace
it.
7) Structure
a) Cabinet body is made up of 1.2mm cold-rolled steel with anti-bacteria powder coating. The
structural strength and stability are enhanced.
b) Work table is made up of 304 stainless steel which provides corrosion resistance as well as
attractive appearance.
c) Base stand is made up of cold-rolled steel with anti-bacteria powder coating.
d) Control panel with soft touch buttons is user-friendly and multifunctional.
2.3 Instructions for Operation
2.3.1 Normal Operation Notice
1) Make sure input voltage is correct and stable. The rated load of main power socket should be
higher than laminar flow cabinet consumption. Plug must be well grounded；
2) Moving principles of different samples inside cabinet: When two or more samples need to be
moved in, be sure that low-polluting samples should be moved first then high-polluting samples.
Movement of items should also follow the principles of moving slowly and steadily.
3) The weight of items placed in the cabinet should not be more than 23Kg/25×25cm2；
4) Avoid vibration: avoid using vibration equipment (eg centrifuges, vortex oscillator, etc.) inside the
cabinet. Vibration would cause lower cleanliness of operating area and affect operator protection.
5) NO FLAME: No flame is allowed inside the Laminar flow cabinet. Using of fire will lead to
airflow disorder, and filter damage. If sterilization is required during the experiment, infrared
sterilizer is highly recommended.
6) HEPA filter life: With the usage time increasing, dust and bacteria accumulate inside HEPA filter.
Filter Resistance is getting bigger, when it reaches the maximum point, the speed requirements can’t
be met. Then need contact BIOBASE service department to get a new one. The used filter should be
processed as medical waste.
7) The fan and its underside is mute bellows, which is sealed strictly in the factory. The operator is
not allowed to remove or loose screws of those parts. If necessary, please contact service personal or
agent.
8) The maximum storage period is one year. If the period is more than one year, performance test
should be done.

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Serious declaration:
NOTE: BIOBASE WILL NOT BE RESPONSIBLE FOR ANY RISK OR DAMAGE ARISING
FAILURE DURING USAGE BY UNTRAINED OPERATOR AND MISHANDLING THE
LAF. THE OPERATOR OF THE LAMINAR FLOW CABINET SHOULD BE TRAINED
ENOUGH BEFORE USING THE LAF.
2.3.2 Operation Process
a) Connect to a suitable power supply
b) Press relevant buttons and check if work normally(functions and operations, pls kindly refer to the
instruction of 2.2.2); Checking the fan, UV lamp and LED lamp if work normally, the air velocity if
meet standard requirements.
c) The cabinet should be sterilized by UV lamp for at least 30 minutes with the window fully closed
before any experiment.
NOTE:
1) For safety of eyes and skin, people should leave the room during UV sterilization.
2) UV lamp should be checked regularly. It should be replaced when either the total working
time reaches 600 hours or the intensity is lower than the requirement.
d) Please move up the front window to proper height above the work table and turn on the blower.
Make sure the experiment should be started after fan working for at least 30 minutes.
For operating safety, please put testing materials inside the cabinet in advance.
After finishing the experiment, please fully close the front window and make sure to sterilize the
cabinet by UV lamp for at least 30 minutes before turning off the cabinet.
2.4 Daily Maintenance
A detailed daily record of operating time is recommended, as the accumulated using time will
directly affect the plan of maintenance.
To avoid electric shock, please cut off ALL power before applying maintenance for the
cabinet!
Preparation before Maintenance: Please remove all the items which are inside the cabinet
Material needed: soap, hot water or warm water, a piece of soft cotton cloth, a piece of dry cloth or
towel, medicinal alcohol or other disinfectants.
2.4.1 Clean the Surface of Working Zone
Wipe the entire surface with a soft cotton cloth which has been soaked with concentrated liquid soap.
Afterwards, wipe off the foam with another cotton cloth or towel which has been soaked with clean
hot/warm water. At the end, wipe the entire surface with a dry cotton cloth or towel rapidly.
For the contaminated or dirty work surface and sump, use medicinal alcohol or other disinfectant to
wipe.
Disinfectants used for wiping should not damage the 304 stainless steel.
2.4.2 Clean the External Surface and Front Window
Use a piece of soft cotton cloth or towel with non-abrasive household cleanser to wipe the surface.

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2.4.3 Overall Maintenance Period
The recommended interval period for comprehensive maintenance is either one year or 1000
working hours.
2.4.4 Maintenance Methods
1) Weekly or daily maintenance
a. Disinfect and clean the operating area(Please refer to 2.4.1 Clean the Surface of Working Zone);
b. Clean the external surface and front window(Please refer to 2.4.2 Clean the External Surface and
Front Window);
c. Check the various functions of the cabinet if abnormal;
d. Record down the maintenance result;
2) Monthly maintenance
a. Clean the external surface and front window(Please refer to 2.4.2 Clean the External Surface and
Front Window);
b. Use towel with 70% medicinal alcohol or 1:100 dilution of household bleach to wipe the working
table, the inner face of front window and the inner wall surface of the working area(exclude the top
wind grid). Use another towel with sterile water to wipe those area to erase the remain of chlorine.
c. Check the various functions of the cabinet if abnormal;
d. Record down the maintenance result;
3) Annual maintenance
a. Check the front window tubular motor, make sure both of them are well connected to the motor
with same tightness.
b. Check the UV lamp and LED light.
c. Apply for overall performance test of the cabinet annually to ensure that the safety meets
requirements. User is responsible for testing costs.
d. Record down the maintenance result.
Cut off the power during maintenance
2.4.5 Storage Conditions
Laminar flow cabinet should be stored in a warehouse with relative humidity no more than 75%, and
temperature lower than 40℃. The warehouse with should have good ventilation performance,
without acid, alkali and or other corrosive gas. Storage period shall not exceed one year. Laminar
flow cabinet stored for more than one year needs to be unpacked and checked before selling and
using. Only the tested and qualified cabinet could be sold.
2.5 Replacement Parts List
BBS-V800 replacement parts list
Number
Name
Specification
BH-01
Fuse
5A
BH-02
Lamp holder T8
LG13-01A
BH-03
UV lamp
T6 20W
BH-04
LED T5 stand
T5 8W
BH-05
UV lamp ballast
1*TL8-18W
BH-06
HEPA filter
720*430*50
BH-07
Fan
FS133C

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BH-08
Control panel
Laminar flow cabinet control
panel(Ten light pillar)
BH-9
Tempered glass
Size 748*580*5
BH-10
Common glass
Size 714*570*5
BH-11
Constant spring
component
Constant spring,Nylon sleeve,Nylon
set of baffle

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2.6 Wiring Diagram (Picture 8)
1 2 3 4
A B C D
4321
D C B A
fan
FUSE1
5A
L
N
mcu
brown（Φ1）
brown（Φ1）
blue（Φ1.0）
bl ue（Φ1）
y e llow gr een（Φ1.0）
transfor m er
light
uv
L
fan
yellow（Φ1）
bl ue（Φ1）
blue（Φ1）
1 2V/ 0. 3A
UV LAMP
UV BAL LAST
BLACK（Φ1） WHITE（Φ 0.5）
BLACK（Φ0.5）
WHITE（Φ0.5）
BLACK（Φ0.5）
BLUE（Φ1）
green（Φ1）
BLUE（ Φ1）
UP
UV
DOWN
+5v
FAN
LIGHT
y e llow gr een（Φ1.0）
LED LAMP
Picture 8

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3. Trouble Shooting and Solutions
3.1 Common Failures and Solutions
Please confirm that the power is well connected, the power cord and fuse are in good
condition(without any damage).
Failure
Checking parts
Measures
LED lamp fail to work
LED stand plug
Connect the plug and stand tightly
LED stand
Replace stand
Circuit
Check the circuit
Control panel
Replace the control panel
UV lamp fail to work
Interlock
Check the blower and LED lamp turn off or not
Lamp holder
Connect the tube and lamp holder tightly
Lamp tube
Replace the lamp tube
Ballast
Replace the ballast
Circuit
Check the circuit
Control panel
Replace the control panel
Button fail to work
Control panel
Make sure the power is well connected and the
fuse is in good condition
Check if the button is broken
Make sure the connecting wire is well
connected
Replace the control panel
Blower fail to work
Blower
Replace the blower if it is damaged
Circuit
Check the circuit
Control panel
Replace the control panel
No electricity in
equipment
Power supply
Check whether the power supply is well
connected
Power cord
Check whether power cord is in good condition
Fuse
Check whether the fuse is damaged
Potential transformer
Check whether the transformer works normally
Control panel
Replace the control panel
Display fail to work
Connection wires
Check connection wires if in good connection
Display screen
Check whether the screen is in good condition
Control panel
Replace the control panel
NOTE:

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1) The above trouble shooting methods should be done by qualified electricians under safe
conditions(cut off power supply). Other components should not be removed. Risk caused by
failing to follow those instructions would be responsible by user.;
2) Please contact Biobase or our agent if a failure could not be traced or solved. Do NOT repair
the equipment without a qualified electrician;
3) The trouble shooting and repair of this equipment only could be undertaken by trained and
recognized technicians;
Please contact Biobase or our agent to order required component or part. The model number
and the serial number of purchased cabinet need to be indicated.
3.2 Replacement of Fuse
Replace the fuse using a flat-blade screwdriver socket pulled out of the fuse holder and replace, then
press it back. (Picture 9)
Picture 9
3.3 Replacement of LED Lamp
When the LED light needs to be changed, turn off the power. Then remove the LED stand, unplug
the right side, After replacing a new LED stand, inserted into the inclined slot.

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Picture 10
3.4 Replacement of UV Lamp
Ultraviolet intensity should be tested regularly in order to achieve a good disinfection effect. We
recommend use Ultraviolet intensity test card to confirm if we need to replace UV lamp. when using
UV lamps reach to the time of 600 hours, we recommend to replace the lamp. When replacing, turn
off the power, and then screw the lamp tube 90 ° and take it off, then replace the correspondence type
of lamp, and put it to the lamp holder and and screw 90 ° in reverse direction.
Picture 11
LED stand
LED stand plug

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3.5 Label Description
3.5.1 Fuse Label (Picture 12)
Picture 12
Note: 5 A fuse label
4. Warranty
4.1 Warranty is 12 months from EX-factory date (excluding consumable accessories, UV and LED
lamp, fuse).
4.2 Biobase would not be liable for any repair of damage caused by improper operation..
4.3 If the warranty has been expired, Biobase would still responsible for repair with relative charges.
4.4 Life time of laminar flow cabinet is 8 years from production date on the label.
4.5 Biobase would provide equipment drawings and necessary technical data for maintenance
companies or personnel trained by Biobase engineers.
Warranty declaration: One-year Warranty, Life-long Maintenance