LI-COR C-DiGit User manual

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2
1
*Used for alternative connection methods, described in detail in the C-DiGit
Blot Scanner User Guide on the Resource CD.
Unpack C-DiGit® Blot Scanner
Place the C-DiGit Blot Scanner away from heat sources (furnaces, windows,
etc.) and out of direct sunlight.
Place C-DiGit Blot Scanner in Lab
Install Image Studio™Software
Windows® 7, 8, 8.1, or 10:
Important: You must be logged in to Windows using an Administrator
account. If necessary, log out and back in using an Administrator account.
1. Place installation CD into drive.
2. If installation does not start immediately, browse to the CD and open
Win_Image Studio Digits_5.x.x.exe.
3. Click Next and follow the instructions in the Setup Wizard.
NOTE: If you are using Windows 10, a separate driver must be installed to
connect your C-DiGit Blot Scanner to Image Studio Software via USB.
Learn more at licor.com/c-digit-driver
Mac OS X 10.9, 10.10, 10.11, or 10.12:
1. Place installation CD into drive.
2. If installation does not start immediately,
browse the desktop and double-click on
MacImageStudioDigits_5.x.x.dmg
3. Click Agree to accept the license agreement.
4. To install, drag the Image Studio icon onto
the Applications icon (right).
LAN USB
Amphenol
12VDC 1A
Remove the yellow warning label on the C-DiGit Blot Scanner back panel.
Remove Warning Label
N. America, Japan
United Kingdom
Australia
Europe
P
U
S
H
LOCK
OPEN
Turn clockwise
to lock
Push button and
turn counterclockwise
to release
The power supply has interchangeable AC plugs for use in North America and
Japan, Australia, Europe, and the United Kingdom. Install the appropriate plug
on the power supply as shown.
Install Power Supply Plug
The power supply has the
North America/Japan plug
pre-installed.
P
U
S
H
LOCK
OPEN
AC Power
C-DiGit Blot Scanner
Computer
USB Connection
C-DiGit
USB Connection
C-DiGit
Power Connection
BSU
12VDC 1A
Connect the larger of the two connectors on the USB cable to your computer,
and the smaller end to the C-DiGit Blot Scanner back panel at the connector la-
beled ‘USB’. Connect the power supply plug to the jack on the rear panel la-
beled ‘12VDC 1A’.
Connect USB Cable and Power
Power the C-DiGit Blot Scanner on with the power switch on the back panel.
The blue power light on the instrument front panel (below) illuminates when
the instrument is powered on.
Turn on C-DiGit Blot Scanner
When the C-DiGit Scanner is powered on and connected
to the computer for the first time, drivers are automati-
cally installed on the computer. Allow up to 5 minutes
for the drivers to install before starting the Image
Studio Software in Step 9 below. NOTE: You may have
to dismiss any ‘Found New Hardware’ messages.
Wait While USB Drivers Install
Double-click the Image Studio icon on the desktop to start
the Image Studio Software.
Start Image Studio Software
PC: Click Create New. Navigate to where the folder will reside and name
the folder. Click Save.
Create New Work Area Folder
The Work Area is simply a folder on the hard drive or network where
all of the images, analyses, and settings are stored.
Mac: Click OK to use the default work area, or click Create New. Name the
folder and navigate to where the folder will reside. Click Save.
Power Supply
and Plugs
Ethernet
Cable*
USB
Cable C-DiGit
Target
Image Studio™
and Resource
CDs

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13
12
11
If there is only one C-DiGit Scanner detected by the computer, it will connect
automatically; if there are multiple C-DiGit Scanners detected, you will have to
choose the desired C-DiGit Blot Scanner from a list of available instruments
(below, listed by serial number).
Connect to C-DiGit® Blot Scanner
You can find the serial number
on a label on the underside of
the instrument.
When a connection is established, the green indicator light will illuminate on
the instrument front panel, and the Status panel in the Image Studio™win-
dow will display ‘Ready’.
NOTE: C-DiGit Blot Scanner uses IPv6 and IPv4 Link-Local Addressing to auto-
matically create an IP network without any user configuration or additional
servers. If you experience connection problems, be sure that IPv6 is enabled
on the computer.
Open the Target envelope and expose the
side of the Target with the gray back-
ground to room light for 30 seconds. Place
the Target face down (gray background)
on the C-DiGit Blot Scanner scanning sur-
face.
Expose C-DiGit Blot Scanner Target
1. Expose to room
light for 30 seconds
2. Place exposed
side down
Expose this side
Acquire Target Image
Make sure that Sensitivity is set to Standard ( ). If Sensitivity is not set to
Standard, click on the Sensitivity button and choose Standard from the pull-
down menu. Image acquisition using Standard sensitivity takes approximately
6 minutes to complete.
Click the Start button ( ) on the Acquire tab ribbon.
Image acquisition will start, and the target image will be displayed as a series of
six images of various brightness/contrasts (Step 14).
Adjust Target Image
Verify that your target image is similar to that shown below. If it matches, your
C-DiGit Scanner is functioning correctly.
The highlighted icons at
right can also be found in
the rightmost pane of the
main Image Studio window,
and will open the Chooser
(right) and Adjust Display
(below) dialogs.
Click on the best image; that image will open in a new window (below), allow-
ing for further adjustment, if desired. Click on Signal,Background, or Midtones
to adjust the brightness for these channels. Click Done.
Click the other ribbon tabs (above) for additional features, including:
Image - Adjust the image appearance, crop, rotate, view image array.
Analysis - Draw shapes to quantify data.
Annotation - Add text annotations to image.
Lab Book - Create a .pdf file containing images, data tables, and charts.
Click on the Shapes and/or Images tabs at the bottom of the window to view:
Shapes - Table of quantification data for selected image.
Images - Table of acquired images, settings, and comments.
Congratulations!
You are now ready to image your own sample. Additional resources can be
found in the C-DiGit Blot Scanner User Guide, on the Resource CD.
www.licor.com/cdigit Doc # 984-19132 05/20
LI-COR, C-DiGit and Image Studio are trademarks or registered trademarks of LI-COR, Inc.
Mac and OS X are trademarks of Apple Inc. All other trademarks belong to their
respective owners.