LI-COR D-DiGit User manual

Gel Scanner
User Guide

CE Marking:
This product (model number 3500) is a CE-marked product. For conformity information, contact
LI-COR Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales
office or distributor.

Notes on Safety
LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety
of this product cannot be guaranteed if the product is used in any other way than is specified in this manual. The D-
DiGit™Gel Scanner is intended to be used by qualified personnel. Read this entire manual before using the D-DiGit
Gel Scanner.
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Notice
The information contained in this document is subject to change without notice. LI-COR MAKES NO
WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO
THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.
LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection with
the furnishing, performance, or use of this material.
This document contains proprietary information that is protected by copyright. All rights are reserved. No part of
this document may be photocopied, reproduced, or translated to another language without prior written consent of
LI-COR, Inc.
Printing History
Publication Number 977-16717
Published May 2017
Global Headquarters
Serving the United States • 4647 Superior Street • Lincoln, NE 68504
Toll free: 800-645-4267 • Fax: [phone] • biosal[email protected]
LI-COR GmbH, Germany
Serving Europe, Africa, and the Middle East.
LI-COR Biosciences GmbH • Siemensstraße 25A • 61352 Bad Homburg • Germany
Phone: [phone] • Fax: [phone] • [e-mail]
LI-COR Ltd., United Kingdom
Serving Denmark, Finland, Iceland, Ireland, Norway, Sweden, and UK
LI-COR Biosciences UK Ltd. • St. John’s Innovation Centre • Cowley Road
• Cambridge • CB4 0WS
United Kingdom • Phone: [phone] • Fax: [phone] • [email protected]
LI-COR Biotechnology Distributor Network: www.licor.com/bio/distributors
www.licor.com/bio
LI-COR is an ISO9001 registered company. © 2017 LI-COR Inc. All rights reserved. Specifications subject to change.
LI-COR, Image Studio, and D-DiGit are trademarks or registered trademarks of LI-COR Inc. SYBR is a registered
trademark of ThermoFisher Scientific; Diamond is a trademark of Promega; GelGreen and GelRed are trademarks of
Biotium; GreenView is a trademark of Applied BioProbes.
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Page
Chapter 1: Safety Information and Initial Setup
About the D-DiGit Gel Scanner 1
FCC Compliance Statement 1
Safety Precautions 2
Initial Setup 4
Unpack the D-DiGit Gel Scanner 4
Computer Requirements for D-DiGit Image Acquisition Software 4
Install D-DiGit Image Acquisition Software 4
Unlock Scanner 5
Connect Power and USB Cables 5
Turn on the D-DiGit Gel Scanner 5
Start D-DiGit Image Acquisition Software 5
D-DiGit Image Acquisition Software Overview 6
Chapter 2: System Overview
Scan Surface and Lid 7
Rear Panel 8
Chapter 3: Operation
Image Gel 9
Position Gel 9
Scan Gel 9
Excising or Observing a Gel 10
Modify D-DiGit Image Acquisition Software Settings 11
Image Folder 11
Gray Mode 11
Resolution 11
Image Name 11
Image Format 12
Show Warning Messages 12
Prepare Scanner for Shipment 12
Chapter 4: Maintenance
D-DiGit™ Gel
Scanner
Table of Contents
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Care and Cleaning 13
1. Turn off the scanner and unplug the power cable from the AC outlet. 13
2. Wipe the top and sides 13
3. Wipe around the scan surface and under the handle 14
4. Wipe the scan surface with deionized water 14
Chapter 5: Appendix
Troubleshooting 15
Specifications 16
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Chapter 1: Safety Information and Initial Setup
About the D-DiGit Gel Scanner
The D-DiGit Gel Scanner is designed to scan gels stained with safe alternatives to Ethidium
Bromide.
The D-DiGit Gel Scanner is intended for use with the following stains:
lSYBR®Safe DNA Gel Stain
lSYBR®Gold Nucleic Acid Gel Stain
lSYBR®Green I Nucleic Acid Gel Stain
lDiamond™ Nucleic Acid Dye
lGelGreen™ Nucleic Acid Gel Stain
lGelRed™ Nucleic Acid Gel Stain
lMidori Green Direct
lMidori Green Advance DNA Stain
lGreenView™ Nucleic Acid Gel Stain
lGreenView™ Plus Nucleic Acid Gel Stain
lGreenView™ Ultra DNA Gel Stain
FCC Compliance Statement
This equipment (model number 3500) has been tested and found to comply with the limits for a
Class B digital device, pursuant to Part 15 of the FCC rules.
These limits are designed to provide reasonable protection against harmful interference in a
residential installation. This equipment generates, uses and can radiate radio frequency energy and,
if not installed and used in accordance with the instructions, may cause harmful interference to
radio communications. However, there is no guarantee that interference will not occur in a
particular installation. If this equipment does cause harmful interference to radio or television
reception, which can be determined by turning the equipment off and on, the user is encouraged to
try to correct the interference by one or more of the following measures:
lReorient or relocate the receiving antenna.
lIncrease the separation between the equipment and receiver.
lConnect the equipment into an outlet on a circuit different from that to which the receiver is
connected.
lConsult the dealer or an experienced radio/TV technician for help.
NOTE: A shielded Hi-Speed USB interface cable with ferrite core installed on the gel scanner
connector end must be used with this equipment.
D-DiGit™ Gel
Scanner
Page 1

Chapter 1: Safety Information and Initial Setup
Caution: Changes or modifications not expressly approved by the manufacturer responsible for
compliance could void the user's authority to operate the equipment.
This device complies with Part 15 of the FCC Rules. Operation is subject to the following two
conditions: (1) This device may not cause harmful interference, and (2) this device must accept any
interference received, including interference that may cause undesired operation.
Safety Precautions
Before using your scanner, read the following safety guidelines carefully. The safety guidelines detail
the proper operation of the scanner and its accessories to prevent injury to users or damage to
equipment.
Safety Icons
This icon indicates a hazardous situation which, if not avoided, could result in injury to users or damage to
equipment.
This icon indicates a hazardous situation which, if not avoided, could result in serious injury or death to
users.
Power Supply
Use a power source with the power rating specified on the marking label on the
scanner.
Turn off the power to each device before connecting or disconnecting cables.
Insert the plug completely into the outlet, as a loose connection may cause arcing and
result in fire.
Hold the plug or connector to disconnect cables. Pulling the cable may damage the
wire and result in fire or electric shock.
Do not locate the scanner where the power cord will be walked on or exposed to water
or chemical spills.
When the scanner is left unattended and unused for long periods of time, unplug it
from the wall outlet.
Moving and Storing the Scanner
Always ensure the scanner is stored properly before shipping or moving it. Quick
stops, excessive force, and uneven surfaces may cause the product to overturn when
moving.
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