Lonza CytoSMART 2 User guide

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CytoSMART™ 2 System Manual
Bioscience Solutions

Content
1 The CytoSMART™ 2 Technology 4
2 Getting Started 5
2.1 What’s in the Box 5
3 Installing the CytoSMART™ 2 Device 6
4 Setting up Your Tablet 8
4.1 Setting up the Internet Connection 8
4.2 Starting a Project 10
4.3 Ending a Project 11
4.4 Running an Offline Project 11
4.5 Cell Counting 12
5 CytoSMART™ Connect Cloud 13
5.1 Opening Project Email 13
5.2 CytoSMART™ Connect Project Page 13
5.3 Image Analysis Settings 16
6 Troubleshooting 18
6.1 No Internet Connection Available 18
6.2 Cannot Connect with CytoSMART™ 2 Device 18
6.3 Cannot Charge Tablet 19
6.4 Video is Shaking 19
6.5 No Project Link Received 19
6.6 Cleaning the CytoSMART™ 2 Device 19
6.7 Removing Dust Particles from Cover Glass 19
7 Technical Specifications 20
8 Warnings 21

WiththeCytoSMART™2System,aneweraofpersonal andaffordablelive
cell imaging systems begins. With the integrated cloud functionality,
cell culture monitoring can occur outside of the lab, in your office or at
home, outside a clean-room or an incubator with defined (e.g. hypoxic)
conditions. Simply view your cell culture online in real-time through
the cloud with any browser-capable device, including your computer,
smartphone, or tablet.
How it Works
Setting up your CytoSMART™ 2 System does not require intensive
training, and can be set up in minutes. The CytoSMART™ 2 System
contains a mini-microscope roughly the size of a cell culture plate
fitting into any incubator. This device is linked to an accompanying
tablet that is fixed outside the incubator. Via this tablet, you can start
CytoSMART™ Projects and capture images of your cell culture. The data
is then transmitted to the CytoSMART™ Connect Cloud via wireless
transmission (WiFi) or Ethernet (LAN).
For each CytoSMART™ Project started, you will receive an email link that
allows you to monitor your cell culture remotely and in real-time.
All image files are intermediately stored on the tablet, in case
your internet access is interrupted, to assure full data safety. The
CytoSMART™ Connect Cloud is hosted via a German cloud hosting
service that currently fulfills some of the world’s strictest and most
secure data storage criteria.
Images and time-lapse videos of your cell culture can easily be
downloaded via the CytoSMART™ Connect Cloud as single images (jpg
files) or as movie files (avi files).
Applications for the CytoSMART™ 2 System
With the CytoSMART™ 2 System your cells can remain in their defined
conditions inside the incubator while you monitor the culture online.
You can also set automatic email alerts so you only need to physically
tend to your cells once they have reached a specified confluency.
Examples of Applications
– Migration assays such as scratch assays
– Documentation of stem cell culture such as reprogramming into
iPSC or for mesenchymal stem cells
– Monitoring and documentation of cell culture under restricted
conditions such as in clean-rooms or under hypoxic cell culturing
conditions
– Determination and documentation of cell growth curves
– Studies to determine influences of various factors on cell culture
performance (e.g. compound addition, screening of culture media
or growth factors)
1 The CytoSMART™ 2 Technology
www.lonza.com/cytosmart
Support Teams
Europe
Scientific Support: [phone]
[e-mail]
North America
Scientific Support: + 1 800 521 0390 (toll free)
[e-mail]
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2.1 What’s in the Box
Ensure the following items are included:
– Manual
– Tablet
– CytoSMART™ 2 Device
– Power adaptor
– Power cord
– Cable clips (3 pieces)
– License key
– USB Ethernet hub
License Key
123456789
Tablet hybrid
USB Ethernet hubLicense key
Power adaptor and
power cord
CytoSMART™2 Device
(incl. attached cable)
2 Getting Started
5
Cable clips (3 pieces)

Items required for installation:
– Incubator
– CytoSMART™ 2 Device
– Tablet
– Power adaptor
– Power cord
– Cable clips
Cable Placement
Check the incubator for a cable opening. If your incubator has one,
thread the CytoSMART™ Cable through the hole. See Figure 1. If no
opening is present, place the CytoSMART™ Cable between the door
and the frame of the incubator. See Figure 2.
3 Installing the CytoSMART™ 2 Device
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Figure 1: Placement in incubator with cable opening Figure 2: Placement in incubator without cable opening

Attachment of Tablet to Incubator Door
The tablet can be attached to the incubator with the Velcro tape in the tablet
box. To do so, peel off the plastic strip of the loop side and stick the tape to
the back of the tablet. Peel off the plastic strip of the hook side. To remove the
tablet from the keyboard base, hold the keyboard and lift one corner of the
tablet to release it. Hold the tablet with the USB port facing the door hinges
(see Figure 3). Press the tablet firmly against the incubator door.
Alternatively, the tablet with attached keyboard can be placed beside the
incubator. Tablet can be closed during a running project as power safe mode
of tablet is disabled.
USB Connection
The cable connected to the CytoSMART™ 2 Device should be connected to the
tablet. Connect the power adaptor to the tablet and the power cord. Plug the
power cord into the electrical outlet (see Figure 4).
Secure Cables
The cables can be secured to the incubator using the three cable clips
provided. It is advised to use one cable clip to secure the connection of the
CytoSMART™ Cable to the tablet. This can be done by placing the cable clip just
behind the USB connector that goes into the tablet (see Figure 5). This will
ensure the connector stays in position when the incubator door is opened.
Indicator Light
To confirm the system is running properly turn on the tablet, open the
incubator and check to see if the light panel around the CytoSMART™ 2 Device
is illumitated:
– Blue light: no project running
– Red light: project running
T-ask Placement
The CytoSMART™ 2 Device accomodates a variety of different culture vessels,
including T-flasks, well plates, single dishes and flat tubes.
It is advised to place the CytoSMART™ 2 Device in the incubator with the LED
holder pointing to the left. This way the live image on the tablet moves in the
directions indicated by the arrows when culture vessels are moved on the
CytoSMART™ 2 Device as shown (see Figure 6).
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Ethernet / LAN
Ethernet / LAN Connection
If WiFi is not present you can connect the CytoSMART™ 2 Device to the internet
using an Ethernet hub (see Figure 7). It is important to follow a specific
connection sequence: Switch on the tablet, disconnect the power adaptor from
the tablet if it is connected, connect the power adaptor with the Ethernet hub,
then connect the Ethernet hub to the tablet. If notification “Please connect
charger” appears, repeat the above steps. Connect the Ethernet cable to your
LAN.
Important: To connect the CytoSMART™ 2 Device to the Ethernet, only use
the Ethernet hub provided with the CytoSMART™ 2 System.
Figure 3: Attachment of tablet to incubator
Figure 5: Secure cables
Figure 4: Connection
Figure 6: T-flask placement and positioning
Figure 7: Ethernet hub connection

Figure 8: Power adaptor and power cord for charging tablet
4 Setting up Your Tablet
IMPORTANT: You must have the CytoSMART™ Cable connected
to the tablet and the tablet connected to a power outlet.
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To set up your system, have the following available:
– Login details of a stable WiFi internet or LAN connection (no
guest network)
– CytoSMART™ 2 Device
– Tablet (connected to power source)
– A valid license key
4.1 Connecting to the Internet
Charging the Battery
Fully charge the tablet battery before using it.
Connect the power adaptor to the tablet and to the power cord. Then
connect the power cord to a pow er outlet.
The power cord varies by country or region. Make sure to only use the
power adaptor and power cord that is supplied in the package. Using a
different charging unit or cable may damage your tablet.
Turning on the Tablet
To turn on your tablet, hold down the power button on the top edge for
a few seconds, then release it.
The tablet manual can be downloaded from the manufacturer’s
website.
Connecting to the Internet
After the CytoSMART™ 2 Software loads, a yellow bar with the message
“Please connect to the internet.” will appear at the top of the screen. To
proceed you first need to connect the tablet to the internet via a WiFi
or LAN connection.
To connect the tablet to the internet swipe from right to left on the
outside of the right side of the screen. Select “Network” (1).
Select your WiFi network (2) and log in.
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How to Minimize the CytoSMART™ App
Use the minimize button to get to the Windows desktop, enabling
access to Windows features and applications like Edge (internet
browser). To minimize the CytoSMART™ App, select the “Menu” button
(3) followed by the “Minimize” (4). To go back to the CytoSMART™ App,
select the application in the task bar.
Starting the CytoSMART™ App
The CytoSMART™ App will launch automatically when the tablet is
turned on.
Entering the License Key
Enter the license key (5) that can be found in the CytoSMART™
Accessories box. Use either the keyboard base or the on-screen
keyboard, which can be opened by pushing the keyboard button at the
display bottom. Read terms and conditions and accept by checking
the check box.
You are now ready to start a project (6).
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4.2 Starting a Project
Place a culture vessel containing cells on top of the CytoSMART™ 2
Device. You should now see the cells on the screen.
Setting Focus
Use the slider (7) to adjust the focus. The plus and minus buttons
can be used for more precise adjustments.
Zoom Functionality
To digitally enlarge the image, use the “Zoom” function. Select “Menu”
then select “Zoom In”. To return the image to standard formatting,
select “Zoom Out”.
Start Recording
To start a recording, select “Project” (8).
Entering a Project Name
Select the “Project Name” field (9) and give your project a name.
Please note that the Project Name field is mandatory. Use either
the keyboard base or the on-screen keyboard which is opened by
pushing the keyboard button at the display bottom. You can add an
Extra Note (10) if desired (e.g. cell type, passage, etc.). Select “Next”.
Entering Your Email Address
Next, enter your email address to receive the project link (11). Select
“Next” (12).
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IMPORTANT: Please ensure the email address is correct, other-
wise you will not receive the email with a link to the project.
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