Lumedica OQ LabScope User manual

User Manual
Version 1.2

Original Instructions Lumedica OQ LabScope 1.2 | Page 1 of
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Contents
Contents ....................................................................................................................................................... 1
Introduction ................................................................................................................................................. 2
Safety ............................................................................................................................................................ 3
General Safety ......................................................................................................................................... 3
Foreseeable Misuse ................................................................................................................................ 3
Care and Maintenance ........................................................................................................................... 3
Residual Risk ........................................................................................................................................... 4
Service ...................................................................................................................................................... 4
Components ................................................................................................................................................ 5
Quick Start (Basic System Only) ................................................................................................................ 6
Quick Start (with Workstation Package) .................................................................................................. 8
Software ..................................................................................................................................................... 10
Main Tab ................................................................................................................................................. 11
Advanced Tab .......................................................................................................................................... 15
Configuration Tab ................................................................................................................................... 17
Hardware ................................................................................................................................................... 18
System Specifications .............................................................................................................................. 18
Mechanical Drawings .............................................................................................................................. 19
Customized OCT System Configuration ................................................................................................ 20
Regulatory ................................................................................................................................................. 22
Warranty .................................................................................................................................................... 23
Customer Support .................................................................................................................................... 24
Certifications and Compliance ................................................................................................................ 25

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Introduction
Please read this instruction manual before operating the OCT imaging
system. All statements regarding safety and any technical specifications only
apply when the system is operated correctly.
This system is intended for laboratory use only and is NOT cleared or
certified for medical applications.
This system contains a superluminescent diode (SLD) which is considered a
Class 1 laser device.
There is laser emission from the lens at the end of the scanner.
The Lumedica OQ Labscope OCT Imaging system is an optical coherence tomography system
for general laboratory, research, and industrial use. A 840 nm superluminescent diode (SLD)
provides the light source. When combined with an interferometer and high-resolution
spectrometer, the system generates optical depth images of samples. System is configured
with a scanner that may be used in either a handheld configuration or mounted into a scanner
stand. An embedded Windows PC provides the user interface for controlling the system and
generating the OCT images. The system will image any samples that can be placed about 1 cm
from the end of the scanner
Thank you for purchasing an OQ LabScope system from Lumedica. Our goal at Lumedica is to
build great OCT systems at affordable prices. We believe that OCT imaging should be available
to all who wish to use it in education, research, and industry.
The team at Lumedica is available to support your work. If you have questions or ideas, please
contact us at the email address or phone number below.
Contact: Lumedica, Inc., 701 West Main Street, Suite 200, Durham, NC 27701 USA
Email: [email protected]
Phone: [phone]
The version number of this manual is 1.2 and it covers the OQ LabScope hardware version 1.1
with software version 1.2.
LASER RADIATION

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Safety
General Safety
 Check the supply voltage and cords before plugging in the system. System power supply must
be plugged in to a grounded outlet (100 -240 VAC, 50 – 60 Hz). If the provided cords do not
match your outlet, please contact Lumedica for a replacement.
 The main unit (or system chassis) requires a supply voltage of 12Vdc and has a maximum power
rating of 60W. This power is supplied by a removable 12Vdc mains supply with power cord.
 If the system has been recently shipped; do not operate until the unit temperature has
equilibrated to room temperature.
 System must be on a flat, dry, and stable surface for operation.
 There are no user serviceable parts inside the scanner or the computer. Operators may make
adjustments to the reference arm inside the OCT engine. Instructions are provided in the section
titled ‘Customized OCT System Configuration’.
 Do not obstruct the air vents on the bottom or top of the system.
 The safety of any system incorporating this system is the responsibility of the assembler of the
combined system.
 If the system is used in a manner not specified by the manufacturer, then protection provided by
the system may be impaired.
 Noise Levels for this equipment were recorded at less than 70 db(A).
Foreseeable Misuse
 Do not use this product outdoors.
 Do not store or operate the system in a damp or closed environment.
 Do not use the equipment in a potentially explosive atmosphere.
 Do not use solvents on or near the equipment.
 Do not use equipment unless trained to do so.
 Do not open any of the sealed electrical enclosures.
 Ensure that the equipment is disconnected from the mains power prior to re-locating, any
maintenance, and/or cleaning.
Care and Maintenance
Please handle the system with care during packing, transportation, and unpacking. Banging or dropping
the system can damage the unit or reduce system performance.
 Do not store or operate the system on surfaces that are susceptible to vibrations.
 Keep away from dust, dirt, and air-borne pollutants.
 The system is not designed for outdoor use. Protect the system from rain, snow, and humidity.
 Do not expose the system to mechanical or thermal extremes.
 The system has a degree of ingress protection of IP32.
 The acceptable environment use conditions for this equipment are:
o Indoor use only.
o Altitude up to 2000 m.
o Temperature range of 5C to 40C.

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o Maximum Relative Humidity (RH) at 80% for temperatures up to 31C, decreasing linearly
to 50% RH at 40C.
o Mains supply voltage fluctuations up to +/- 10% of nominal voltage.
o Transient over-voltages up to the level of overvoltage category II.
o Temporary over-voltages occurring on the mains supply.
o Applicable pollution degree of intended environment is PD2.
 Cleaning optional monitor – monitor screen may be cleaned with an oil-free, lint free type cloth.
 Cleaning the system chassis – the chassis may be wiped down with an oil free, lint free cloth.
Canned air duster may be used to blow dust off of main unit.
 Cleaning scanner – scanner may be wiped down with oil free, lint free cloth. Canned air duster
may be used to blow dust off of scanner including optics. If optics becomes dirty or smudged,
such as with fingerprints, please contact Lumedica customer service.
Residual Risk
Please note the following residual risks associated with this equipment.
 Possibility of electrical shock if/when working inside electrical enclosures.
 Possibility of ergonomic injury when moving the equipment. The main unit (or system chassis)
weighs 4.0 kg, the scanner weighs 0.5 kg, and the optional scanner stand weighs 3.0 kg.
Service
Only trained and approved Lumedica personnel are allowed to service the system. Please contact
Lumedica support for more information.

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Components
These are your OQ LabScope system hardware components.
Power Cable
Embedded Computer
System Chassis
Scanner

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Quick Start (Basic System Only)
Carefully open the shipping box and unpack all items. Contents include:
 User Manual
 OQ LabScope system with handheld scanner
 Power supply with 12Vdc cord
 Appropriate country cord for 12Vdc power supply (US, EU, AU, IN, and UK)
 Roll of Scotch™ tape
If any items are missing, please contact us directly at [e-mail].
1. Set up OQ LabScope system within a meter of an electrical outlet.
2. Select country cord for your location and plug into 12Vdc power supply.
3. Plug power supply into wall outlet.
4. Plug 12 Vdc power cord into back of OQ LabScope box.
5. You will need to supply a monitor with a resolution of 1920 x 1080 and an HDMI
interface with cord. The software interface is designed for this resolution and may not
work at other resolutions.
6. You will need a keyboard and mouse. We recommend using a combination wireless or
Bluetooth keyboard/mouse so that only one USB port is required.
7. Make sure that the handheld scanner cable is laid flat and not twisted or kinked.
8. Make sure scanner is in secure location and will not fall off of lab bench.
9. Power up OQ LabScope by pushing the button on top of the box.
10. Windows 10 OS will boot up and take you to the login screen.
11. Login username is: oqlabscope
12. Login password is: oqlabscope
13. Start OQ LabScope software by double clicking on Lumedica OQ LabScope icon.

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14. Software will come up on “Main” tab.
15. Push “Start Scan” button on “Main” tab.
16. OCT image will begin updating.
17. Push “Update Background” to minimize horizontal noise in image.
18. Place Scotch™ tape under handheld scanner and position scanner and tape so that top
of tape is near to the top of the OCT image.
19. Adjust the focus slider on the left side of the image to optimize.

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Quick Start (with Workstation Package)
The Workstation Package contains the following additional contents:
 Monitor
 HDMI cable for monitor
 Appropriate country power cord for
monitor
 Wireless keyboard
 Wireless mouse
 USB dongle for keyboard and mouse
(inside mouse battery cover)
 Scanner stand
(includes base, plate for base,
vertical stage with travel, Allen keys,
and holder for scanner)
 Adapter for integrating handheld
scanner into scanner stand
If any items are missing, please contact us directly at [e-mail].
1. Set up OQ LabScope box within a meter of an electrical outlet.
2. Select country cord for your location and plug into 12Vdc power supply.
3. Plug power supply into wall outlet.
4. Plug 12Vdc power cord into back of OQ LabScope box.
5. Set up monitor.
6. Connect HDMI cable to back of monitor and then to back of OQ LabScope box.
7. Connect country power cord to monitor.
8. Plug power cord into mains outlet.
9. Plug USB dongle into either USB port on top of OQ LabScope box.
10. Set up scanner stand.
11. Vertical stage is attached to baseplate with two screws.

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12. Holder for scanner attaches to vertical stage with one screw. Note that holder should be
orientated with arm at the bottom, otherwise scanner will be too high above the sample.
13. Place plate in base.
14. Insert scanner into scanner adapter and secure with the provided screws.
15. Insert scanner with adapter into holder on scanner stand.
16. Power up monitor by pressing power switch.
17. Power up OQ LabScope by pushing the button on top of the box.
18. Windows 10 OS will boot up and take you to the login screen.
19. Login username is: oqlabscope
20. Login password is: oqlabscope
21. Start OQ LabScope software by double clicking on Lumedica icon.
22. Software will come up on “Main” tab.
23. Push “Start Scan” button on “Main” tab.
24. OCT image will begin updating.
25. Push “Update Background” to minimize horizontal noise in image.
26. Place Scotch™ tape under handheld scanner and position scanner and tape so that top
of tape is near to the top of the OCT image.
27. Adjust the focus slider on the left side of the image to optimize.
28. Image should look like this:

Original Instructions Lumedica OQ LabScope 1.2 | Page 10 of 26
Software
The software is started by clicking on the L icon on the taskbar.

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Main Tab
This tab provides the interface needed to run the software. Software functionality is organized
by tabs: Main, Processing, Advanced and Configuration
Buttons:
Start Scan/Stop Scan — Used to start and stop the acquisition of OCT images.
Update Background — Available when the system is acquiring OCT images, i.e. the
Start/Stop button says “Stop Scan”. When this button is pressed the system will acquire a
background frame which is then subtracted from all subsequent frames. As the system drifts
over time, horizontal lines will appear in the OCT image. Updating the background will minimize
these horizontal lines. Remove the sample before updating the background so that the OCT
image does not become part of the background.
Save B-Scan Image — Available when the system is not acquiring OCT images, i.e. the
Start/Stop button says “Start Scan”. When this button is pressed, the system will save the
displayed image to a .tif and a .jpg file. The files are saved to C:\Users\Public\Documents\
Lumedica\OctEngine\Data and are named “BSCAN-SGL-#date#-#time#” where #date# is the
current date and #time# is the time when the file was saved.
Capture C-Scan — Available when the system is not acquiring B-Scan OCT images, i.e. the
Start/Stop button says “Start Scan”. When this button is pressed, the system will initiate a
single C-Scan with the default value of 32 B-Scans per C-Scan. The number of B-Scans per C-
Scan is selectable on the Configuration tab. The images are stored in the image buffer and can
be saved to disk using the “Save All Images” button on the Processing tab.

Original Instructions Lumedica OQ LabScope 1.2 | Page 12 of 26
Sliders:
Focal Value — Located to the left of the OCT image, this slider controls the liquid lens in the
scanner. Moving the slider up and down sets the focus position of the OCT scanning beam.
When the slider is at the top of the screen, the focus is closest to the scanner and when the
slider is at the bottom of the screen the focus is farthest away from the scanner.

Original Instructions Lumedica OQ LabScope 1.2 | Page 13 of 26
Processing Tab
This tab contains the image processing controls. This functionality is only enabled when the
OCT system is not scanning. That is, the Start/Stop button on the Main tab says “Start Scan.”
The OQ LabScope software maintains a circular buffer of the last 30 images acquired by the
system. This corresponds to about 3 seconds of imaging time.
Buttons:
Average Scans — Averages all of the scans that are in the image buffer. Displays the result in
the right hand panel on this tab.
Save Single Image — Stores the selected single B-Scan image that is displayed in the left-
hand panel to disk. This image is saved in both .tif and .jpg formats. The directory where the
files are saved is C:\Users\Public\Documents\Lumedica\OctEngine\Data. The files are named
“BSCAN-SGL-#date#-#time#” where #date# is the date and #time# is the time when the file
was saved.
Save Average Image — Stores the average B-Scan image that is displayed in the right-hand
panel to disk. This image is saved in both .tif and .jpg formats. The directory where the files are
saved is C:\Users\Public\Documents\Lumedica\OctEngine\Data. The files are named “BSCAN-
AVG-#date#-#time#” where #date# is the current date and #time# is the time when the file
was saved.
Save All Images — Stores all B-Scan images in the image buffer to disk. These images are
saved in .jpg format. The files are saved to a new folder in
C:\Users\Public\Documents\Lumedica\OctEngine\Data\#date#-#time# where #date# is the

Original Instructions Lumedica OQ LabScope 1.2 | Page 14 of 26
current date and #time# is the time when the files were saved. The files are named “BSCAN-
SGL-#number#” where #number# is the number of the image in the buffer, zero-padded to
three digits (e.g. 001).
Sliders:
Single B-Scan Dynamic Range Bottom — Located to the left of the Single B-Scan OCT
image, this slider controls the bottom value of the intensity range of this image. Sliding this all
the way to the bottom will typically make the image look brighter and noisier. Sliding this
toward the top will typically make the image look blacker and less noisy, but will start to
suppress the image signal. As the slider approaches the top of the range, only the most intense
areas of the image will still be visible.
Average B-Scan Dynamic Range Bottom — Located to the right of the Average B-Scan
OCT image, this slider controls the bottom value of the intensity range of this image. The effect
is similar to the slider on the left, however, the averaged image is typically less noisy, so it may
be possible to set the slider to a lower value while still maintaining a good image.
Single B-Scan — This slider at the bottom of the Single B-Scan OCT image selects which
image from the image buffer is being displayed. The image buffer contains up to 30 images
when capturing B-Scans and all of the B-Scans captured when acquiring a C-Scan.

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Advanced Tab
This tab provides additional control over how the OCT image is acquired and processed.
Buttons:
Start Scan/Stop Scan — Start and stop the acquisition of OCT images.
Update Background — Available when the system is acquiring OCT images, i.e. the
Start/Stop button says “Stop Scan”. When this button is pressed the system will acquire a
background frame which is then subtracted from all subsequent frames. As the system drifts
over time, horizontal lines will appear in the OCT image. Updating the background will
minimize these horizontal lines. Remove the sample before updating the background so that
the OCT image does not become part of the background.
Save B-Scan Image — Available when the system is not acquiring OCT images, i.e. the
Start/Stop button says “Start Scan”. When this button is pressed, the system will save the
displayed image in both .tif and .jpg formats. The directory where the files are saved is
C:\Users\Public\Documents\Lumedica\OctEngine\Data. The files are named “BSCAN-SGL-
#date#-#time#” where #date# is the current date and #time# is the time when the file was
saved.
Capture C-Scan — Available when the system is not acquiring B-scan OCT images, i.e. the
Start/Stop button says “Start Scan”. When this button is pressed, the system will initiate a
single C-Scan acquisition with the default value of 32 B-Scans per C-Scan. The number of B-
Scans per C-Scan is selectable on the Configuration tab. The images are stored in the image
buffer and can be saved to disk using the “Save All Images” button on the Processing tab.

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Save Raw B-Scan Image — Available when the system is not acquiring OCT images, i.e. the
Start/Stop button says “Start Scan”. When this button is pressed the system will save a copy of
the raw B-scan data from the spectrometer to a file. The image format is .tif and is in the
directory C:\Users\Public\Documents\Lumedica\OctEngine\Data. The files are named “BSCAN-
RAW-#date#-#time#” where #date# is the date and #time# is the time when the file was
saved. The current background subtraction data is also saved with the name “BSCAN-BKGD-
#date#-#time#”.
Save All Raw Images — Available when the system is not acquiring OCT images, i.e. the
Start/Stop button says “Start Scan”. When this button is pressed the system will save a copy of
all the raw B-Scan data in the queue from the spectrometer to a set of files. The image format
is .tif and they are saved to a new folder in
C:\Users\Public\Documents\Lumedica\OctEngine\Data\#date#-#time# where #date# is the
current date and #time# is the time when the files were saved. The files are named “BSCAN-
RAW-#number#” where #number# is the number of the image in the buffer, zero-padded to
three digits (e.g. 001). The current background subtraction data is also saved in this folder
with the name “BSCAN-BKGD”.
Sliders:
Focal Value — Located to the left of the OCT image, this slider controls the liquid lens in the
scanner. Moving the slider up and down sets the focus position of the OCT scanning beam.
When the slider is at the top of the screen, the focus is closest to the scanner and when the
slider is at the bottom of the screen the focus is farthest away from the scanner.
Dynamic Range Bottom — This slider on the right side of the image sets the bottom value of
the intensity range of the left-hand image. Sliding this all the way to the bottom will typically
make the image look whiter and noisier. Sliding this toward the top will typically make the
image look blacker and less noisy, but will start to suppress the image signal. As the slider
approaches the top of the range, only the most intense areas of the image will still be visible.
Dispersion Compensation B — Sets the second order coefficient in the dispersion
compensation. This may be adjusted while the system is running to optimize the value.
Dispersion Compensation C — Sets the third order coefficient in the dispersion
compensation. This may be adjusted while the system is running. Typically, the value of this
coefficient is close to zero.

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Configuration Tab
Buttons:
Scan Direction — Set to either “Horizontal” or “Vertical”. The vertical scan direction is aligned
with the long side of the handheld scanner. The horizontal scan direction is aligned with the
short side of the handheld scanner. When the scanner is placed in the scanner stand, the
horizontal direction is left to right scanning and the vertical scan is front to back scanning.
Sliders:
Scan Width — Sets the relative length of the B-Scan. The longest scan is “1” and it may be
shortened down to “0”, at which point the scanner is not moving. This will give a time series at
one point, which is referred to as an M-mode scan. A scan length of 1 corresponds to 7
millimeters of scan range. All B-scans have the same number of A-scans, i.e. a shorter scan of
a curved surface will make the image appear flatter.
B-Scans Per C-Scan — Set the number of B-Scans captured in a C-Scan. Options are 32, 64,
128, 256, and 512. C-Scan capture time scales with the number of B-Scans and can be as long
as 120 seconds for a C-scan with 512 B-Scans.

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Hardware
System Specifications
Image
Size
512px x 512px A-Scan
Line Rate
8,800/second Output
Power
750 μW max.
Depth
Resolution
7 μm in air, 5 μm in tissue B-Scan
Image Rate
12/second System
Size
33w x 19d x 15h cm.
13w x 7.5d x 6h in.
Transverse
Resolution
15 um C-Scan
Image Rate
Set by number of B-
scans in C-scan
Scanner
Size
11w x 6.4d x 4.8h cm.
4.5w x 2.5d x 1.9h in.
Center
Wavelength
840 nm Linear Scan
Range
7 mm System
Weight
4.0 kg.
Sensitivity
(OSNR)
100 dB C-Scan
Range
5mm x 5mm Scanner
Weight
0.5 kg.

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Mechanical Drawings
System Chassis
Scanner