Lumencor RETRA User manual

Lumencor Light Engine Manual
RETRA (L) !
Light Engine"
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Regulatory Models
Lumencor utilizes regulatory model names for all certified and CE marked products. The regulatory model names are
traceable to all regulatory documentation, third party reports and certifications.
“Regulatory Model: Retra III L” is used as a representative model for all certified and CE marked RETRA (L) laser products.
Emissions
This equipment has been tested and found to comply with the limits of EMC directive 2014/30/EU and FCC part 15 (CISPR
11:+A1:2016). These limits are designed to provide reasonable protection against harmful interference when the equipment is
operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful interference to radio communications.
Safety Certifications
TUV SUD America, CB Certification (IEC 61010-1:2010)
TUV SUD America, NRTLus Certification (UL 61010-1:2012-05)
TUV SUD America, cNRTL Certification (CAN/CSA-C22.2 No. 61010-1:2012)
TUV SUD America, EN Certification (EN 61010-1:2010)
Underwriters Laboratories (UL), CB Certification (IEC/EN 60825-1:2014 Safety of laser products)
CE Marking
Low Voltage Directive (2014/35/EU)
EMC Directive (2014/30/EU)
RoHS Directive (2011/65/EU+2015/863/EU)
REACH Regulation (EC) No. (1907/2006/EC)
EU Declarations of Conformity can be found at https://lumencor.com/company/compliance
For EU customers discarding end-of-life Lumencor electrical and electronic equipment: Please submit an RMA request with
“Recycle product under WEEE” in the Description of Issues field.
For disposal in countries outside of the European Union: This symbol is only valid in the European Union (EU). If you wish to
discard this product, please contact your local authorities or dealer and ask for the correct method of disposal.
Lumencor, Inc. | 14940 NW Greenbrier Parkway | Beaverton, OR 97006 USA | 503.213.4269 | www.lumencor.com!
Revision D!
011822!
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Lumencor Light Engines as supplied, and as represented in this manual, meet safety and regulatory requirements!For
Research Use Only.!If the light engine is incorporated into an instrument or system for a specific end-use application,
it is the responsibility of the system integrator to verify that the light engine, and the system into which it is
incorporated, meet all safety and regulatory requirements of that end-use application.

Table of Contents!
1. Introduction
2. Precautions and Warnings
3. Installation and Operating Instructions
4. Light Output Characteristics
5. Operational Specifications
6. Routine Maintenance and Trouble Shooting
7. Customer Support
8. Warranty
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1. Introduction
The RETRA (L) Light Engine consists of 1 or 2 individually addressable solid-state light sources with integrated
electronic control systems. The constituent light sources may be LEDs, luminescent light pipes or lasers. Optimal
internal operating temperature is maintained by negative pressure air cooling with the air intake at the front of the
light engine and the exhaust fan at the the rear. The outputs of the constituent light sources are refined by
bandpass filters and merged into a common optical train directed to the light output port on the front panel. The
light output port has a built-in adapter for connection to an SMA-terminated optical fiber or a liquid light guide
(LLG). An onboard photodiode continuously monitors the light output and generates a reference signal that is
applied to the constituent sources in a feedback loop to maintain constant light output over time. The light
sources within the RETRA (L) Light Engine®are controlled by software via one of two serial interfaces, USB/
RS-232 or TCP, to the on board computer operating Lumencor firmware. The user can enable or disable each
source independently by serial commands as well as change the intensity of each source independently. The
RETRA (L) Light Engine can either be controlled by a Lumencor GUI, by third party microscopy data acquisition
software or by a LAN connection to an onboard computer with an embedded command library. Alternatively, the
light sources may be turned on and off by TTL inputs from a trigger device such as a camera. The only manual
control is the master power switch on the front panel to turn on/off the electrical power into the unit.
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2. Precautions and Warnings {Précautions et mises en garde}
A few simple practices will ensure trouble-free operation for the life of the light engine.
Les quelques règles simples suivantes permettront d’assurer un fonctionnement fiable pendant toute la durée de
service de la source lumineuse.
Safety Instructions:
Please read and follow all safety instructions provided BEFORE using the RETRA (L) Light Engine®. Failure to
comply with the safety instructions may result in fire, electrical shock, or personal injury and may damage or impair
protection provided by equipment. Please save all safety instructions.
Instructions de sécurité:
Veiller à lire et à respecter toutes les instructions de sécurité fournies AVANT d’utiliser le RETRA (L) Light Engine®
afin d’écarter les risques d’incendie, de décharge électrique, de blessure corporelle et de possibles dommages ou
défaillance de la protection offerte par l’appareil. Conserver toutes les instructions de sécurité.
Safety Definitions {Définitions relatives à la sécurité}:
Warning: Statements identify conditions or practices that could result in personal injury.
Avertissement: déclarations qui identifient des situations ou des pratiques susceptibles d’entraîner
des blessures corporelles.
Caution: Statements identify conditions or practices that could result in damage to your equipment.
Attention: déclarations qui identifient des situations ou des pratiques susceptibles d’endommager le matériel.
Safety Items {Mesures de sécurité}:
Warning: ONLY use the power supply provided by Lumencor. The Lumencor-supplied 24 VDC, 9.2 A
external power supply is required for use with the RETRA (L) Light Engine. The light engine is required to be
supplied by an approved/certified DC power source meeting the minimum electrical ratings of the product. The
DC power supply must have the AC power cord connected to a receptacle with a protective safety (earth) ground
terminal.
Avertissement: utiliser uniquement l'alimentation fournie par Lumencor. Le Lumencor fourni 24 VDC/9.2 A
alimentation externe est recommandé pour une utilisation avec le moteur de lumière RETRA (L) . Le moteur léger
doit être alimenté par une source d'alimentation CC approuvée/certifiée répondant aux caractéristiques
électriques minimales du produit. L'alimentation CC doit avoir le cordon d'alimentation CA connecté à une prise
avec une borne de terre de sécurité (terre).
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Warning: DO NOT look into the output of the light engine. The brightness of this light source is higher than
most commercial lighting fixtures and is intended to couple directly into a microscope or other bioanalytjcal
instrument.
Avertissement: NE PAS regarde directement la sortie de la source lumineuse. L’intensité lumineuse de
cette source est supérieure à celle de la majorité des appareils d’éclairage disponibles dans le commerce et est
conçue pour un raccordement direct à un microscope ou autre appareil de bioanalyse.!
Warning: DO NOT turn on the light without!the output end of the light guide safely!directed!into an
enclosed optical path. #DO NOT point the light output directly onto any flammable or burn-susceptible
material.#This includes all animal or vegetable tissues, plastics, fabrics, paper and liquids.!
Avertissement: NE PAS allumer la lumière sans l'extrémité de sortie du guide de lumière dirigée en
toute sécurité dans un chemin optique fermé. NE PAS pointer la sortie de lumière directement sur un matériau
susceptible d'être inflammable ou susceptible de brûler. Cela comprend tous les tissus, les plastiques, les tissus, le
papier et les liquides animaux ou végétaux.
RISK GROUP 3
Warning:
Possibly
hazardous
optical radiation
emitted from this
product. Do not
look at operating lamp. Eye injury may result.
Warning: Infrared (IR) emitted from this product. Do not look at operating lamp.
Warning: UV emitted from this product. Avoid eye and skin exposure to unshielded product.
GROUPE DE RISQUE 3
Avertissement: Infrarouge (IR) émise par ce produit. Ne regardez pas la lampe d'exploitation.
Avertissement: Rayonnement optique Peut-être dangereux émis par ce produit . Ne regardez pas la lampe
d'exploitation. Une blessure oculaire peut entraîner.
Avertissement: UV émis par ce produit . Évitez les yeux et la peau exposition au produit non blindé.
Caution: Use of controls or adjustments or performance of procedures other than this specified herein may result
in hazardous radiation exposure.
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Attention: L'utilisation de commandes ou de réglages ou l'exécution de procédures autres que celles spécifiées
dans le présent document peuvent entraîner une exposition à des radiations dangereuses.
Class 4 Laser Warning
Warning: This product contains Class 4 laser sources. Avoid eye and
skin exposure to direct or scattered visible and invisible laser radiation.
Avertissement: Ce produit contient des sources laser de classe 4.
Évitez l'exposition des yeux et de la peau au rayonnement laser visible
ou dispersé visible et invisible.
Laser Aperture Warning
or
Warning: Avoid exposure - laser
radiation is emitted from this
aperture. Do not turn on light engine without first connecting a light guide to the output aperture. !The distal end
of this light guide must be coupled into an enclosed optical path prior to operation. Each operational control and
laser aperture that can be separated by 2m or more from a radiation warning device shall itself be provided with a
radiation warning device. Do not exceed 2m without providing a radiation warning device in accordance with EN
60825-1:2014.
Avertissement: Évitez l'exposition - le rayonnement laser est émis à partir de cette ouverture. L'ouverture de
sortie est interverrouillée et un guide de lumière doit être connecté et couplé dans un chemin optique inclus avant
l'opération. Chaque commande opérationnelle et l'ouverture laser qui peuvent être séparées de 2 m ou plus à
partir d'un dispositif d'avertissement de rayonnement doivent être munies d'un dispositif d'avertissement de
Laser Sources
Color
Center Wavelength (nm)
Power
Beam Divergence
See label located on
product.
300-1150nm. See label located
on Product.
See label located on
product.
Beam divergence shall be
calculated at the end-system.
Refer to CoC for the light
engine beam divergence (NA)
at the output aperture.
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rayonnement. Ne dépassez pas 2m sans fournir un dispositif d'avertissement de rayonnement conformément à la
norme EN 60825-1:2014.
Warning: DO NOT open the unit. There are no serviceable parts inside and opening the light engine enclosure
will void the manufacturer’s warranty.
Avertissement: NE PAS ouvrir l’appareil. Il ne contient aucune pièce réparable et l’ouverture de son boîtier a
pour effet d’annuler la garantie.
Caution: DO NOT set liquids on the light engine. Spilled liquids may damage your light engine.
Attention: NE PAS placer de liquide sur la source lumineuse. Les liquides renversés peuvent endommager la
source lumineuse.
Caution: DO NOT drop the light engine. It contains glass optical components that could be damaged or
misaligned by the shock produced by a drop onto a hard surface.
Attention: NE PAS laisser tomber la source lumineuse. Elle contient des composants optiques en verre
susceptibles d’être endommagés ou désalignés par le choc résultant d’une chute sur une surface dure.!
DISCLAIMER: Lumencor shall not be liable for injury to the user or damage to the product resulting
from the RETRA (L) Light Engine®being used in a way for which it was not intended and in complete
disregard for any posted safety precautions and warnings.
AVIS DE NON-RESPONSABILITÉ: Lumencor décline toute responsabilité pour les blessures corporelles
ou les dommages au produit résultant d’une utilisation du RETRA (L) Light Engine®autre que celle
prévue et du mépris total de les mesures de sécurité et mises en garde affichées.
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3. Installation and Operating Instructions
3.1 Contents!
The RETRA (L) Light Engine ships with the following list of standard components:!
1. The RETRA (L) Light Engine®engine, configured with 1 to 2 output channels (colors) and an
output adapter for connection to an SMA-terminated optical fiber or a liquid light guide (LLG)
as documented on the certificate of conformance (Figure 1).!
2. A 24 V/9.2 A DC power supply (Lumencor part number
27-10019).!
3. A region-specific AC power cord for the power supply (see
adjacent table).!
4. RJ45 ethernet cable.!
5. Control key, external gate jumper and remote interlock jumper
(Figure 2).!
6. Quickstart Guide instruction document (85-10049).!
!
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AC Power Cords
Region
Part Number
North America
29-10002
Europe
29-10005
United Kingdom
29-10004
Israel
29-10008
Australia/New Zealand
29-10024
Figure 1. Example certificate of conformance (C of C)
for RETRA (L) Light Engine. The C of C identifies the
color channels installed in the light engine and the
bandpass filters associated with each channel. Full
(100%) power outputs measured at the terminus of the
liquid light guide (LLG) or optical fiber are recorded in the
third column,
Figure 2. External gate jumper, remote interlock jumper, and
control keys. These item are packed in a plastic bag inside the
shipping box. Their functions are described in Section 3.3.1.
EXAMPLE

The model name, unique 4- or 5- digit serial number and
certification markings of the light engine are carried on a
label affixed to the rear panel. Performance specifications
for individual light engines are listed on the certificate of
conformance included with the shipping documents e-
mailed to the customer (Figure 1).!
3.2 Installation
NOTE: Any end-product/system incorporating or coupled
to a Lumencor Light Engine shall be fully evaluated to
verify all applicable safety and regulatory compliance
requirements prior to use. !
When setting the RETRA (L) Light Engine up for use, place
the unit on a hard surface and avoid blocking or restricting
airflow at the air inlet (front panel; Figure 3) or exhaust
ports (rear panel) on the enclosure. Restricting the airflow
will cause the unit to operate at elevated temperatures and
will result in decreased product life and/or premature
failure. !
The RETRA (L) Light Engine must be operated in a non-
condensing environment (dew point <10ºC with controlled
ambient temperature <30º C). Thermal overload protection
is provided by the on-board computer in conjunction with
an on-board temperature sensor. If the internal
temperature registered by the sensor exceeds 50°C OR the
fan rotor is stopped, all light output channels automatically
turn OFF and are locked in this state until the internal
temperature is below 50°C and/or the fan restarts. The
current reading of the on-board temperature sensor is
displayed on the front panel status display (Figure 3) and in
the GUI (Figure 5).!
Connect the DC power supply to a grounded AC wall outlet
using the power cord supplied with the light engine.
Connect the DC output to the 6-pin receptacle on the front
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Figure 3. RETRA (L) Light Engine front panel.
Figure 4. AURA (L) Light Engine rear panel.
Remote
interlock
connector (with
jumper installed)

of the light engine (Figure 3). Turn the light engine on using the master power switch. The
embedded green indicator will illuminate (if applicable, ensure that the liquid light guide is inserted
and secured; see below).!
The RETRA (L) Light Engine®may be configured for light delivery via a 3 mm liquid light guide or an
SMA-terminated optical fiber. The output couplers are built into the light engine front panel and are
not field-exchangeable (Figure 3). The 3 mm liquid light guide output coupler incorporates an
electrical power interlock switch in the back of the receptacle. The liquid light guide must be fully
inserted in the receptacle to activate the interlock. Electrical power supply to the light engine cannot
be turned on via front panel power switch until the interlock is activated. After fully inserting the
liquid light guide, lock it in position using the set screw on the side of the receptacle to prevent
inadvertent disconnection of the light guide during use.!
3.3 Operation
3.3.1 Control and Interlock Keys
The Master Power Switch button on the front panel (Figure 3) turns the electrical power to the unit
on or off. A green power indicator embedded in the button is lit when the power supply is
connected to the light engine and the power button is in the on position. Initialization of the onboard
computer takes about 30 seconds after the master power switch is turned on. When initialization is
complete, the status indicator display (Figure 3) will activate.!
The Key Control (Figure 3) must be in the on position before light output can be turned on. The key
must be removed and stored in a secure location when the product is not in use. ONLY trained
individuals should use and have access to the key. The Master Power Switch button, Key control
and Remote interlock can be used to shut offlight output.!
The Source Enabled indicator LED (below the status indicator display; Figure 3) provides a warning
indication that one or more light sources are active and emitting invisible and/or visible radiation.!
The Remote Interlock Connector: (Figure 4) is provided to allow for connection of a remote
interlock. When this interlock is open it will shut offlight output. After the interlock has been
opened, the Manual Reset button will need to be pushed to resume light output.!
WARNING:#The Remote Interlock relies on a passive continuity circuit for proper operation.#It does
not provide a voltage source for external circuitry, nor can external voltage sources be applied to it.
UNDER NO!CIRCUMSTANCES should a voltage or voltage source be applied to the Remote
Interlock circuit.##APPLYING A VOLTAGE OF EITHER POLARITY MAY RESULT IN ANOMALOUS
OPERATION AND/OR DAMAGE THE PRODUCT, AND COULD IMPACT SAFETY.
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Customers planning to connect multiple Light Engine remote interlocks together will need to contact
Lumencor prior to integration.#INTERCONNECTING LIGHT ENGINES OR USING THE REMOTE
INTERLOCK INCORRECTLY CAN RESULT IN ANOMALOUS BEHAVIOR AND/OR DAMAGE TO
THE PRODUCTS.!
AVERTISSEMENT!:#Le verrouillage à distance repose sur un circuit de continuité passif pour un
fonctionnement correct. Il ne fournit pas de source de tension pour les circuits externes, et aucune
source de tension externe ne peut lui être appliquée. EN AUCUN CAS, une tension ou une source
de tension ne doit être appliquée au circuit de verrouillage à distance. L'APPLICATION D'UNE
TENSION DE POLARITÉ OU DE POLARITÉ PEUT ENTRAÎNER UN FONCTIONNEMENT
ANOMAL ET/OU ENDOMMAGER LE PRODUIT, ET POURRAIT AVOIR UN IMPACT SUR LA
SÉCURITÉ.
Les clients prévoyant de connecter plusieurs verrouillages à distance Light Engine ensemble devront
contacter Lumencor avant l'intégration. L'INTERCONNEXION DE MOTEURS LÉGERS OU
L'UTILISATION INCORRECTE DU VERROUILLAGE À DISTANCE PEUT ENTRAÎNER UN
COMPORTEMENT ANOMAL ET/OU ENDOMMAGER LES PRODUITS.
WARNING: Prior to turning the light output on, be sure the output end of the liquid light guide or
optical fiber is safely directed into an enclosed optical path (e.g. a beam dump).!
AVERTISSEMENT!: avant d'activer la sortie de lumière, assurez-vous que l'extrémité de sortie du
guide de lumière liquide ou de la fibre optique est dirigée en toute sécurité dans un chemin optique
fermé (par exemple, une décharge de faisceau).!
Note: In the event of ANY normal or abnormal interlock fault condition (including high ESD/EMP/EFT
conditions ~2kV) you MUST clear the latch fault condition, either by pressing the manual reset
button or by cycling the power switch.!
3.3.2 Start Up
1. Insert the light guide and secure it with the set screw .!
1
2. Insert the external gate jumper (Figure 2) in the labeled socket on the front panel (Figure 3). Insert
the interlock jumper in the labeled socket in the lower left corner of the rear panel .!
2
3. Insert the control key, turn it to the ON position.!
4. Connect the isolated DC power supply to the light engine.!
5. Connect the AC power cord to the DC power supply.!
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6. As soon the DC power supply is energized, the master power button (top right) will automatically
light up.#The light engine automatically starts when the power is connected; there is no need to
push the master power button .!
3
7. Wait 30–45 seconds for the initiation sequence (onboard microprocessor boot-up) to complete.#
Do not press any buttons or insert any plugs during this time.!
8. When the initiation sequence completes, “LUMENCOR” will flash on front display panel and then
be replaced by a display showing the current light engine IP address, the internal temperature
and the fan status.#At the same time, the fan will come on at HI for about 2 seconds and then
shut offautomatically. The light engine is now ready for use. Prior to turning the light output on,
be sure the output end of the liquid light guide or optical fiber is safely directed into an enclosed
optical path (e.g. a beam dump).!
Notes:
1Ensure that the distal end of the light guide or optical fiber is safely directed into an enclosed
optical path (e.g. microscope epilluminator or a beam dump) before turning the light output on. If
the light engine is equipped with an output adapter for an SMA-terminated optical fiber, connect the
fiber to the output adapter using the integral threaded sleeve. !
2To enable light output of the RETRA (L) Light Engine the external gate jumper, control key, remote
interlock jumper, and Liquid light guide must be inserted. !
3For subsequent start ups, use the master power button to start or shut down the light engine.
Shut down can also be accomplished using the “Shut Down” button in the GUI (Figure 5, left).!
3.3.3 Ethernet Connection and Control GUI
The Web GUI provides a quick and easy way to control the light engine, providing the ability to turn
each source on/off(Figure 4, Control Tab [left], 2), adjust the power of each source independently
from offto full power (Figure 5, Control Tab [left], 3). The GUI also tracks the total on time (Figure 5,
Settings tab [right], 13) and the power sensor readings (Figure 5, Control tab [left], 4). To set up the
Web GUI, follow the follow protocol below:!
1. Go to Start menu > Control Panel > Network & Internet and/ or Network & Sharing Center!
2. Click Change Adapter Settings!
3. Right-click on Local Area Connection!
4. Click on Properties (in pop-up)!
5. Select Internet Protocol Version 4 (TCP/IPv4)!
6. Click Properties button!
7. Use the following IP addresses:!
•IP Address: 192.168.201.201!
•Subnet Mask: 255.255.255.0!
•Default gate way and DNS Server are OK to leave blank!
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8. Connect the RJ45 cable (supplied with the light engine) between the LAN port on the light engine
and an Ethernet port on the computer.!
9. Type the Light Engine IP address (Figure 3) into any web browser address bar to access the
resident control GUI. The factory default IP address is 192.168.201.200.!
RETRA (L) Light Engines that are operating on software version 2.1.19 and above have a standby
mode (Figure 5, Control Tab [left], 6) to conserve power during periods when no active light output
generation is required. The light engine automatically switches into standby mode after a latency
period )i.e. after the last light output = OFF command was issued). The default latency period is 300
seconds (5 minutes), this can be temporarily reset by the user by using the “WAKEUP” command in
the command line (Figure 5, Setting Tab [right], 10). Standby mode is also marked by an automatic
shut-offof the main cooling fan. Standby mode automatically terminates when the next light output
= ON command is issued.!
Output power regulation allows users to eliminate variations in light output due to temperature
fluctuations and other environmental factors in photometric and quantitative imaging applications
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Figure 5. RETRA (L) GUI, Control Tab (Left) and Settings Tab (Right). The GUI is accessed by typing the light engine IP
address (198.168.201.200 in this example) into a web browser. 1) Lumencor light engine model number and software
version, 2) ON/OFF control of source channel, 3) Intensity slider, 4) Live power read out in mW, 5) power regulation controls:
locked in when padlock and input box are grey, 6) Count down timer to Standby mode, 7) Control mode setting, 8) serial
number of light engine, live temperature read out, and live power draw read out, 9) Status indicator buttons, 10) Command

where reproducibility and accuracy are essential. To use power regulation, a desired power reference
value in milliwatts is entered in the onboard control GUI (Figure 5, Control tab [left]). To activate
power regulation, simply click the padlock icon next to the reference power value (Figure 5, Control
Tab (Left), 5). Gray shading of the padlock icon and the reference power value shows that power
regulation is active for the selected output channel. When power regulation is active, the intensity
setting for the channel is controlled by the onboard microprocessor, based on feedback from the
light engine’s reference photodiode array. The microprocessor continuously adjusts the intensity
setting so that the output power matches the power reference value set by the user.!
3.3.4 Control via Serial ports
RETRA (L) Light Engines have two serial ports, labeled USB and RS-232 (Figure 3), which can be set
to receive either LEGACY or STANDARD mode commands. Connection to the computer requires a
USB-A-to-USB B cable (29-10058) or USB-to-RS-232 cable
(29-10011). LEGACY commands are limited to controlling on/off
switching and intensity adjustment of individual color channels. Only
one of the two ports can be set to LEGACY mode at one time. The
STANDARD mode command set gives access to an extensive panel of
operating status reports and configuration settings in addition to the
the basic control functions of the LEGACY command set. A complete
listing of STANDARD mode commands is provided in Lumencor Light
Engine Command Reference (Document number 57-10018). Note that
LEGACY and STANDARD mode communications use different serial
protocols (9600,8,N,1 and 115200,8,N,1 respectively). Changes to the
command mode setting for a serial port can be made via the Settings
screen of Web GUI (Figure 5, right). Changes are applied
instantaneously and are retained between power cycles.!
Select the command mode setting for the serial port that is compatible
with the light engine device driver in the control software. This
selection is typically found under the “Devices” tab. The COM port
address assigned by the computer to the light engine USB serial port
must be correctly registered in control software.!
3.3.5 Control Light Engine Control Pod
1. Connect the RETRA (L) Light Engine to pod using USB A-to-USB B
cable (29-10058).!
2. Open the Web GUI control interface as described in Section 3.3.2.!
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Figure 6. BNC breakout cable to
enable TTL triggering
TTL Connector Pin Definitions
OUTPUT
COLOR
Pin#
OUTPUT
COLOR
Pin#
RED
1
BLUE
12
GREEN
2
VIOLET
13
CYAN
3
nIR
14
TEAL
11
YELLOW
15
Gnd
6,7,8,10
Vihigh (min) = 2.0V, Vihigh (max) = 5.5V
Vilow (min) = 0.0V, Vilow (max) = 0.8V

3. Go to the SETTINGS page (Figure 5, right) in the Web GUI. Make sure that the USB port
configuration is set to LEGACY mode and USB 5V is set to ENABLED.!
4. The pod must be set in the “RETRA III” Light Engine control mode. The light engine control mode
setting is shown in green letters at the bottom of the pod display screen. If the pod is not in the
RETRA (L) Light Engine control mode, change the setting by holding down the MODE button on
the pod until the light engine selection menu appears. Move the cursor to “RETRA III” in the
selection menu by turning the pod control knob. Press the MODE button again to select the
RETRA (L) Light Engine control mode and return to the main control screen.!
5. Follow instructions on Lumencor’s Light Engine Control Pod Operation sheet (54-10036). In brief,
press the COLOR button to select output light output channel, press the MODE button to toggle
light output on and off, turn the control knob to adjust intensity for the current light output
channel selection.!
3.3.6 TTL Control
The TTL Interface provides users with a faster method of switching color channel outputs on and off.
Individual TTL inputs are provided for each color channel, as shown in the adjacent table. As a
safeguard against unintended light output when the inputs are initially connected, the light engine
TTL port is disabled and must be enabled by a serial command. To enable the TTL input, click the
Enabled button next to “TTL inputs” in the GUI under the Settings tab (Figure 5, Settings Tab [right],
12) or send the standard mode command SET TTLENABLE to the LAN port. Input TTL signals can
be conveniently addressed using an accessory BNC breakout cable (Lumencor part no. 29-10156 or
29-10216; Figure 6) connected to the front panel TTL port. The breakout cable also provides a
global shutter input (labeled “shutter”). TTL signals input to the global shutter will synchronously
toggle all currently enabled source channels on and off.!
4. Light Output Characteristics
RETRA (L) Light Engines may incorporate different numbers and types of solid state light sources.
Constituent light sources may include LEDs, luminescent light pipes or lasers. The number of light
sources installed and the bandpass filters associated with each are shown on the Certificate of
Conformance included with the shipping documents e-mailed by Lumencor to the customer. The
certificate of conformance also shows full (100%) power outputs for each source measured at the
terminus of the liquid light guide (LLG) or optical fiber (Figure 1). Not all sources may be turned on
simultaneously. To prevent exceeding the capacity of the DC power supply, power consumption is
tracked by the on-board computer. If a set limit (see Governor, Figure 5, Settings tab [right], 11) is
exceeded, either by increasing intensity settings for sources that are already on, or by turning on
additional sources, commands will be rejected. To clear the error condition, reduce intensities of
sources that are on, or turn offone or more sources.!
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Light source outputs are refined by bandpass filters. The specifications of these bandpass filters are
recorded on the certificate of conformance as CWL/FWHM where CWL =center wavelength and
FWHM = full width at half-maximum transmission, both measure in nanometers (nm). Bandpass
filters are not user-exchangeable and changes require return of the light engine to Lumencor’s
factory for service (see Section 7). Since changing bandpass filters will result in source output
power changes, a new certificate of conformance will be provided as part of this service.!
5. Operational Specifications
The RETRA (L) Light Engine®must be operated and stored within the environmental conditions
specified in the table below. Performance specifications for individual light engines are listed on the
certificate of conformance included with the shipping documents e-mailed to the customer (see
example shown in Figure 1). It is important to retain the certificate of conformance for reference. In
the event that the light engine is sold, the certificate of conformance should be transferred to the
new owner. Certificates of conformance are also recorded in Lumencor’s database and copies can
be requested by e-mail to [e-mail]. The request message must include the 4- or
5-digit serial number of the light engine.!
Specification
Detail
Temperature
Operating
32 to 95° F (0 to 35° C)
Non-operating
-4 to 158° F (-20 to 70° C)
Humidity
Operating and non-operating
0 to 80% relative humidity, non-condensing¹
Dew Point
Operating
32 to 59° F (0 to 15° C)
Altitude
Operating
0 to 10,000 feet (3,048 meters)
Non-operating
0 to 45,000 feet (13,176 meters)
Dimensions
Size (W x L x H)
145 mm x 190 mm x 203 mm (5.7 in x 7.5 in x 8.0 in)
Weight
4.3 kg /9.4 lbs
Lifetime
Time for light engine output to decrease to 70% of the values recorded on the
original certificate of conformance𝟤
Power supply
24VDC / 9.2A
Warm-up Period
1 s
Protection
Thermal overload (Section 3.2). Power overdraw (Section 4)
Sound Level
Sound level at 1 meter < 65db(A)
Control Interfaces
USB, RS-232, TCP, TTL
Warranty
24 months parts and labor
¹The RETRA (L) light engine must be operated in a non-condensing environment.
²The corresponding number of days/months/years may vary considerably depending on the duty cycle implemented by
the user and the prevailing environmental conditions during operation.
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6. Routine Maintenance and Trouble Shooting
No routine maintenance is required. There are no user-serviceable components or sub-assemblies
in the RETRA (L) Light Engine. Opening the light engine enclosure will void the manufacturer’s
warranty. In the event that the light engine fails to perform in accordance with the specifications
listed on the certificate of conformance, please contact Lumencor Technical Support for assistance,
as directed in Section 7.!
7. Customer Support
For technical support of the RETRA (L) Light Engine, please contact Lumencor by phone at
503-213-4269 or through e-, mail at [e-mail]. Please be prepared to provide
the 4- or 5-digit serial number of the light engine. Any light engine returned to Lumencor for repairs
or upgrades requires a pre-issued return material authorization (RMA) number. To request an RMA
number, fill out and submit the online request form. It is the customer’s responsibility to properly
package and safely ship products to Lumencor. Instructions for shipping will be provided in the e-
mail giving notification of the RMA number.!
Troubleshooting Procedures
Problem
Check the following
No response to serial (USB or
RS-232) commands
Check that the LEGACY or STANDARD command mode selection (GUI
Settings; Figure 5 [right]) is compatible with the software driver.
No response to TTL trigger
commands
Check that TTL inputs are ENABLED (GUI Settings; Figure 5 [right], 12) and
that the TTL polarity setting is consistent with the trigger inputs. Also
check that all serial ON/OFF controls are in the OFF state.
No light output in response to
source ON command (serial or
TTL).
There are 4 interlocks that need to be closed, check all of these:!
1. Control key must be inserted in front panel and turned to “on” position¹.!
2. Liquid light guide must be inserted in light output receptacle¹.!
3. Remote interlock jumper must be inserted in rear panel¹.!
4. External gate jumper must be inserted in front panel (Figure 3).
Unusually weak fluorescence
signals across all detection
channels
Weak fluorescence in all detection channels (DAPI, FITC, TRITC, Cy5 etc) is
likely to be due to poor light transmission by the liquid light guide, the
collimating adaptor or another distal component of the microscope optical
path and not to abnormally low light output from the light engine.
Unusually weak fluorescence
signals in a single detection
channel (e.g. DAPI)
1. Check that the light engine power output reading in the Web GUI (Figure
4) is normal.!
2. Check that the dichroic beamsplitter and emission bandpass filter in the
microscope are compatible with light engine excitation filter
specifications shown on the certificate of conformance (Figure 1)𝟤.
High, spatially uniform fluorescence
background
Check that the dichroic beamsplitter and emission bandpass filter in the
microscope are compatible with light engine excitation filter specifications
shown on the certificate of conformance (Figure 1)𝟤.
¹Open interlocks for these conditions will be indicated by a red status indicator in the lower right corner of the
GUI control screen (Figure 5). !
²Excitation filters are installed in the light engine. Excitation filters installed in the microscope cube are generally
redundant and it is recommended that they are removed.
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8. Warranty
RETRA (L) light engines ®are backed by a 24 month warranty to end users. Warranty coverage
starts on the original date of shipment from Lumencor. Light Engines qualifying for warranty service
must be verifiably delivering performance that is substantially at variance with the levels documented
in the certificate of conformance. The light engine must also have been used and maintained under
operating conditions consistent with the specifications given in Section 5, and observing all the
Precautions and Warnings notified in Section 2. This warranty does not extend to light engines that
have been subject to misuse, accident, tampering or improper installation. Accessories including
(but not limited to) liquid light guides, optical fibers, collimators, cables and control consoles are not
covered by the warranties attached to light engines. Please fill out and submit the online warranty
registration form . This will facilitate provision of warranty service should it be required.!
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