Lumencor Spectra FIA 5-NKJ-SA User manual

Emissions Certifications
This equipment has been tested and found to comply with the limits of EMC directive 2014/30/EU. These limits are
designed to provide reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful interference to radio
communications. !
Safety Certifications
Models: !
Spectra FIA 5-NKJ-SA!
Spectra FIA 5-NKJ-SH!
Spectra III 5-NKJ-SA!
TUV SUD America, CB Certification (IEC 61010-1:2010)!
TÜV SÜD America, NRTLus Certification (UL 61010-1:2012/R:2016-04)!
TÜV SÜD America, cNRTL Certification (CAN/CSA-C22.2 No. 61010-1:2012/U2:2016-04)"!
TÜV SÜD America, EN Certification (EN 61010-1:2010)"!
CE Marking
Low Voltage Directive (2014/35/EU)!
EMC Directive (2014/30/EU)!
RoHS Directive (2011/65/EU + 2015/863/EU)!
REACH (Regulation (EC) No. (1907/2006/EC)!
EU Declarations of Conformity can be found at https://lumencor.com/company/compliance !
Revision History
Lumencor, Inc. | 14940 NW Greenbrier Parkway | Beaverton, OR 97006 USA | 503.213.4269 | www.lumencor.com!
Revision C!
011822!
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Revision
Number
File Name
Date
Revised
Page
Revision Summary
4
57-10008 Rev. C Spectra FIA 092121
Sept. 21st, 2020
Cover, 1-12
New logo and style, updated
regulatory information, updated
hyperlinks, swapped sections 6
and 7, formatting
3
57-10008 Rev. B Spectra FIA
052220
May 22nd, 2020
1
Modify revision history
2
57-10008 Rev. B Spectra FIA
042320
April 23rd, 2020
1
Add revision history
11
Addition of call out symbols “ “ to
Light Output Control
1
57-10008 Spectra FIA 042020
April 20th, 2020
11
add Light Output Control (in 7.
Operational Specifications)
0
57-10008 Spectra FIA 033118
March 31st, 2020
-
Create a new entry
1

Table of Contents!
1. Introduction!
2. Precautions and Warnings!
3. Installation and Operating Instructions!
4. Spectral Distribution and Power Output!
5. Temporal Response!
6. Operational Specifications!
7. Routine Maintenance and Trouble Shooting!
8. Customer Support!
9. Warranty !
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1. Introduction!
The Spectra FIA light engine® consists of 5 individually addressable light sources with 4 individual LEDs and 1
luminescent light pipe. The output of these individual sources are combined with dichroics such that the light from
each channel illuminates the entrance port of the two fiber bundles. These light sources are precisely mounted on a
common heat sink. The circuit boards are mounted below the heat sink. The exhaust fan pulls fresh air from the
front of the light engine across the heat sink fins and exhaust the heated air out the rear panel.!
The light sources within the Spectra FIA light engine are controlled by software; via one of two serial interfaces,
RS-232 or TCP, to the onboard computer operating Lumencor firmware. The user can enable or disable each
source independently by serial commands as well as change the intensity of each source independently. The only
manual control is a power switch on the front panel to turn on/offthe power into the unit. The Spectra FIA light
engine can either be controlled by a Lumencor GUI or by a LAN connection to an onboard computer with an
embedded command library.!
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2. Precautions and Warnings {Précautions et mises en garde}!
A few simple practices will ensure trouble-free operation for the life of the light engine.!
Les quelques règles simples suivantes permettront d’assurer un fonctionnement fiable pendant toute la durée de
service de la source lumineuse.!
Safety Instructions:
Please read and follow all safety instructions provided BEFORE using the Spectra FIA light engine®. Failure to
comply with the safety instructions may result in fire, electrical shock, or personal injury and may damage or impair
protection provided by equipment. Please save all safety instructions.!
Instructions de sécurité:
Veiller à lire et à respecter toutes les instructions de sécurité fournies AVANT d’utiliser le Spectra FIA light engine®
afin d’écarter les risques d’incendie, de décharge électrique, de blessure corporelle et de possibles dommages ou
défaillance de la protection offerte par l’appareil. Conserver toutes les instructions de sécurité.!
Safety Definitions {Définitions relatives à la sécurité}:
Warning: Statements identify conditions or practices that could result in personal injury.!
Avertissement: déclarations qui identifient des situations ou des pratiques susceptibles d’entraîner
des blessures corporelles. !
Caution: Statements identify conditions or practices that could result in damage to your equipment. !
Attention: déclarations qui identifient des situations ou des pratiques susceptibles d’endommager le matériel.!
Safety Items {Mesures de sécurité}:
Warning: DO NOT use an unapproved power supply. An external power supply is required for use with the
Spectra FIA light engine®. The power supply used must be capable of supplying 24VDC, 14A. The equipment is
required to be supplied by an approved/certified DC power source meeting the minimum electrical ratings of the
Spectra FIA Light Engine.!
Avertissement": NE PAS utiliser une alimentation non approuvée . Une alimentation externe est nécessaire pour
une utilisation avec le Engine® de lumière Spectra FIA . L'alimentation utilisée doit être capable de fournir 24VDC ,
14A . L'équipement doit être fourni par un certifié DC réunion approuvé / source d'énergie les notes électriques
minimales de la FIA Lumière Engine Spectra FIA .!
Warning: DO NOT look into the output of the light engine. The brightness of this light source is higher than most
commercial lighting fixtures and is intended to couple directly into a microscope or other bioanalytjcal instrument.!
Avertissement: NE PAS regarde directement la sortie de la source lumineuse. L’intensité lumineuse de cette
source est supérieure à celle de la majorité des appareils d’éclairage disponibles dans le commerce et est conçue
pour un raccordement direct à un microscope ou autre appareil de bioanalyse.!
Warning: DO NOT turn on the light without"the output end of the light guide safely"directed"into an enclosed
optical path. "DO NOT point the light output directly onto any flammable or burn-susceptible material."This
includes all animal or vegetable tissues, plastics, fabrics, paper and liquids.!
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Avertissement: NE PAS allumer la lumière sans l'extrémité de sortie du guide de lumière dirigée en toute
sécurité dans un chemin optique fermé. NE PAS pointer la sortie de lumière directement sur un matériau
susceptible d'être inflammable ou susceptible de brûler. Cela comprend tous les tissus, les plastiques, les tissus, le
papier et les liquides animaux ou végétaux.!
RISK GROUP 2
Caution: Possibly hazardous optical radiation emitted from this product. Do not stare at operating
lamp. May be harmful to the eyes.!
!
Caution: Infrared (IR) emitted from this product. Do not stare at operating lamp.!
GROUPE DE RISQUE 2
Avertissement: Rayonnement optique Peut-être dangereux émis par ce produit . Ne fixez pas la lampe
d'exploitation. Une blessure oculaire peut entraîner.!
Avertissement: Infrarouge (IR) émise par ce produit. Ne fixez pas la lampe d'exploitation.!
Caution: DO NOT open the unit. There are no serviceable parts inside and opening the light engine enclosure will
void the manufacturer’s warranty.!
Attention: NE PAS ouvrir l’appareil. Il ne contient aucune pièce réparable et l’ouverture de son boîtier a pour effet
d’annuler la garantie.!
Caution: DO NOT set liquids on the light engine. Spilled liquids may damage your light engine.!
Attention: NE PAS placer de liquide sur la source lumineuse. Les liquides renversés peuvent endommager la
source lumineuse.!
Caution: DO NOT drop the light engine. It contains glass optical components that could be damaged or
misaligned by the shock produced by a drop onto a hard surface.!
Attention: NE PAS laisser tomber la source lumineuse. Elle contient des composants optiques en verre
susceptibles d’être endommagés ou désalignés par le choc résultant d’une chute sur une surface dure.!
DISCLAIMER: Lumencor shall not be liable for injury to the user or damage to the product resulting from the
Spectra FIA light engine®being used in a way for which it was not intended and in complete disregard for
any posted safety precautions and warnings.
AVIS DE NON-RESPONSABILITÉ: Lumencor décline toute responsabilité pour les blessures corporelles ou
les dommages au produit résultant d’une utilisation du Spectra FIA light engine®autre que celle prévue et du
mépris total de les mesures de sécurité et mises en garde affichées.!
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3. Operating Instructions!
3.1 Contents!
The Spectra FIA light engine ships with a 24 V/14 A DC Power Supply and DC power cable as follows:!
The model name, unique 4- or 5- digit serial number and
certification markings of the light engine are carried on a label
affixed to the rear panel (Figure 2). Performance specifications for
individual light engines are listed on the certificate of conformance
included with the shipping documents e-mailed to the customer.
Figure 3 shows the external dimensions of the Spectra FIA light
engine and the location of the air intake and exhaust. Also shown
are the locations of the light output ports A and B and the power
supply and communications port connectors.!
3.2 Installation!
NOTE: Any end-product/system incorporating or coupled to a
Lumencor Light Engine shall be fully evaluated to verify all
applicable safety and regulatory compliance requirements prior to use.!
Spectra FIA Model
Light Engine Part Number
DC Power Supply
DC Power Cable
Spectra FIA 5-NKJ-SA
90-10176
27-10024
29-10098
Spectra FIA 5-NKJ-SH
90-10293
27-10026
29-10103
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Figure 1. DC power cable (Part Number 29-10098)
Lead
Wire length
(mm)
+
3000
–
3000
GND
350
Figure 2. Example identification label located
on rear panel of the Spectra FIA light engine.

When setting the Spectra FIA light engine up for use, place the unit on a hard surface and avoid blocking or
restricting airflow at the air inlets (front panel) or exhaust ports (rear panel) on the enclosure. The light engine must
be operated in a non-condensing environment (dew point <10ºC with controlled ambient temperature <30º C.
Restricting the airflow will cause the unit to operate at elevated temperatures and will result in decreased product life
and/or premature failure. Thermal overload protection is provided by the on-board computer in conjunction with an
onboard temperature sensor. If the internal temperature registered by the sensor exceeds 50°C OR the fan rotor is
stopped, all light output channels automatically turn OFF and are locked in this state until the internal temperature is
below 50°C and/or the fan restarts. The current reading of the on-board temperature sensor is displayed in the GUI
(Figure 5) or can be accessed using the GET TEMP software command. Normal exhaust airflow (with all light
sources operating at 100%) has been measured at 52.7 m3/hour with a temperature 6°C above ambient.!
Connect the DC power supply to the power input port (front panel, lower left corner) using the DC power cable
(Lumencor part number 29-10098 or 29-10103). See Figure 2 for pin connections. Position the unit in such an
orientation that allows unrestricted access to the DC power connector. In an emergency, you may ned to
disconnect power to the unit quickly. Connect the DC power supply to the AC utility power.!
!
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Figure 3. Spectra FIA light engine schematic

The Spectra FIA light engine®has two identical light output ports A and B built directly into the light engine front
panel (Figure 3). The ports are custom designed for connection to Nikon’s fiber bundles. Each fiber bundle should
be inserted all the way into the correct port and then 3 screws are used to mechanically attached the fiber bundle to
the light engine. The insertion depth is set at the factory for optimum performance.!
3.3 Operation!
The only manual control is a power switch on the front panel to turn the electrical power to the unit on or off. A
green power indicator on the front panel is lit (“ON”) when the power supply is connected to the Spectra FIA light
engine®and the power switch is in the on position. Prior to turning the light output on, be sure the output end of the
fiber bundle is safely directed into an enclosed optical path (e.g. a beam dump). The Spectra FIA light engine®can
either be controlled by a Lumencor GUI or via the built in library of software commands. A complete listing of the
commands is provided in the Lumencor Light Engine Command Reference (Document Number 57-10018). The
software commands can be transmitted via the RS-232 port or the LAN input. The default IP address for LAN
communications is 192.168.2.1. The current IP address can be determined using the GET IP command sent over
RS-232. The RS-232 port conforms to the standard RS-232 interface protocol using standard RS-232 connector
pin definitions. The default RS-232 port parameters are 9600,8,N,1. The RJ-45 port enables communication using
Transmission Control Protocol (TCP). The GUI provides a quick and easy way to control the light engine providing
the ability to turn each source on/off, adjust the power of each source independently from offto full power. The GUI
also tracks the total on time and the power sensor readings. The GUI is accessed by typing in the IP address into a
web browser (Figure 4).!
!
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Figure 4. Spectra FIA light engine control GUI screenshot

4. Light Output!
Spectral distribution and power measurements are measured at the distal end of the Nikon–supplied fiber bundle.
Guaranteed minimum output A power values are given in the adjacent table along with an output spectrum
measured from one of the first production light engines (Figure 5). The two peaks in the red output band have an
intensity ratio of 0.7 or more relative to the intensity of the stronger peak normalized to a value of 1.0.!
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400 500 600 700 800 900
Wavelength (nm)
0
5
10
15
20
Intensity (mW/nm)
SN8908 Output A Spectra
Cyan
Green
Red
NIR I
NIR II
Figure 5. Representative spectral output of Spectra FIA light engine
Color
Output A
Power (mW)*
Output B
Power (mW)*
Cyan
370
37
Green
886
88
Red
210
21
NIR I
188
19
NIR II
208
21
TOTAL
1862
186
*Guaranteed minimum output power. Specific output
A power measurements for individual light engines
are recorded on the certificate of conformance
included in the shipping documents e-mailed to the
customer.

5. Temporal Response!
The time to switch all 5 channels simultaneously was measured to be approximately 1.5 msec. The oscilloscope
traces in Figure 6 show the initial trigger of the TCP pulse which is followed by the light pulse turning on or turning
off.!
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Figure 6. Temporal response to TCP commands. The oscilloscope trace marked in green
represents the light output detected by an analog photodiode.

6. Operational Specifications!
The Spectra FIA light engine®must be operated and stored within the environmental conditions specified.!
%
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Specification
Detail
Ambient Temperature
Operating
32 to 95° F (0 to 35° C)
Non-operating
-4 to 158° F (-20 to 70° C)
Humidity
Operating and non-operating
0 to 80% relative humidity, non-condensing
Altitude
Operating
0 to 10,000 feet (3,048 meters)
Non-operating
0 to 45,000 feet (13,176 meters)
Dimensions (LxWxH)
390 mm x 125.20 mm x 170.60 mm
Weight
15.5 lbs / 7.05 kg
Lifetime
Designed for > 20,000 hours light output*. ‡ Contains no components with MTBF
less than 7 years.
Light Output Control
Output light intensity commands
The light sources are turned OFF when the output light intensity value is set to 0%.
Command examples:
The light sources are turned off
SET MULCHINT 0 0 0 0 0
Command for 50% output light intensity
SET MULCHINT 2048 2048 2048 2048 2048
Command for 100% output light intensity
SET MULCHINT 4095 4095 4095 4095 4095
Definition of turning off:
Light intensity monitor value is 0[digit]
Command:
GET MULCHINT
A MULCHINT 0 0 0 0 0
Zero-cross emission point
The zero-cross point of the light output is between 0.5 and 2.5% intensity setting
(see Figure 7).
Figure 7. Diagram of light emission zero-cross point.

7. Routine Maintenance and Trouble Shooting!
Since this light engine will be used in a clean room, no maintenance will be required regarding the air intake and
exhaust. There are no user-replaceable components or sub-assemblies in Spectra FIA light engine®. Opening the
light engine enclosure will void the manufacturer’s warranty.!
8. Customer Support!
For technical support of the Spectra FIA light engine, please contact Lumencor by phone at 503-213-4269 or
through email at techsupport@lumencor.com. Please be prepared to provide the 4- or 5-digit serial number of the
light engine (see Figure 2). Any light engine returned to Lumencor for service requires a manufacturer’s issued return
material authorization (RMA) number. An RMA number must be issued before returning a unit to Lumencor. To
request an RMA number, fill out and submit the online request form. It is the customer’s responsibility to properly
package and safely ship products to Lumencor. Instructions for shipping will be provided in the e-mail giving
notification of the RMA number.!
9. Warranty!
The Spectra FIA light engine®family of light engines is backed by an 18 month warranty. Warranty coverage starts
on the original date of shipment from Lumencor. Light Engines qualifying for warranty service must be verifiably
delivering performance that is substantially at variance with the levels documented in the certificate of conformance.
The light engine must also have been used and maintained under operating conditions consistent with the
specifications given in Section 6, and observing all the Precautions and Warnings notified in Section 2. This
warranty does not extend to light engines that have been subject to misuse, accident, tampering or improper
installation. Accessories including (but not limited to) liquid light guides, optical fibers, collimators, cables and
control consoles are not covered by the warranties attached to light engines. Please fill out and submit the online
warranty registration form . An extended warranty option is available; extended warranties must be purchased in
advance of receipt of goods or within 90 days of the light engine delivery.!
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Continued from page 11
Specification
Detail
Input Power Requirements
Power supply, max voltage
24 VDC, 14 A, maximum internal voltage and current draw
Warm-up Period
use dependent
Protection
thermal overload (see Section 3.2)
Shock and Vibration Resistance
Light engine and packaging pass ISTA 3A test
Sound Level
to be determined
Connectivity
RS-232, TCP
Default IP address
192.168.2.1
Warranty
18 months parts and labor
*Pulse mode light output with one color on at a time at 100% intensity. ‡ Based on information available at the date of
publication. MTBF = mean time between failure.
1
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