Metrohm ProfIC Vario 6 Anion User manual

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Application Bulletin 376
Installation Instruction for ProfIC Vario 6 Anion
The ProfIC Vario 6 Anion is a Professional IC Vario system
with Inline Dilution and Inline Ultrafiltration for the fully
automatic determination of anions or cations with sequential
suppression in very high concentrations in matrices
containing particles. This system enables users to dilute the
sample and eliminate bacteria or inorganic particles prior to
the analysis.
Table of contents
1. Delivery Package...................................................2
IC...........................................................................2
Detector.................................................................2
Sample processor..................................................2
Accessories...........................................................2
Optional.................................................................2
2. Installation..............................................................2
2.1. Installation of the software.....................................2
2.2. Accessory Kit: Vario/Flex Basic (6.5000.000)........2
2.3. Accessory Kit: Vario/Flex ONE (6.5000.010).........2
2.4. 858 Professional Sample Processor......................2
2.4.1. Liquid Handling Station (LQH Station)...................3
2.4.1.1. Rinsing unit............................................................3
2.4.1.2. Dilution unit and 800 Dosino..................................3
2.4.2. Ultrafiltration cell ....................................................4
2.5. Interconnection of devices.....................................4
2.6. 940 Professional IC Vario......................................5
3. MagIC Net..............................................................5
3.1 Configuration..........................................................5
3.2 Method adjustment ................................................6
3.3. Purge of the system...............................................7
3.4. User defined determination....................................7
4. Exemplary measurement.......................................8
5. Optional equipment................................................9
5.1. Eluent Production Module......................................9
5.2. Alternative MSM rinsing and
regeneration methods............................................9

Application Bulletin 376
Installation Instruction for ProfIC Vario 6 Anion
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1. Delivery Package
Delivered with ProfIC Vario 6 Anion package:
2. Installation
Following is a detailed description of how to install ProfIC
Vario 6 Anion.
We strongly recommend that the individual steps are carried
out in the order given below.
2.1. Installation of the software
All programs must be shut down first. Make sure no Metrohm
instrument is connected to the PC. Install MagIC Net with the
help of the MagIC Net - CD. The Microsoft InstallationWizard
is accepted and executed. All the standard directories
proposed by the program should be accepted. Restart
windows.
Now as soon as you connect a new Metrohm instrument via
USB connection to the PC, the driver is installed and a
window will pop up in MagIC Net, asking you if you’d like to
store this device in your configuration. If you do, please
answer with yes. The names will be checked later in this
installation instruction.
2.2. Accessory Kit: Vario/Flex Basic (6.5000.000)
Using the Accessory Kit Vario/Flex Basic, install the
Professional IC Vario. Remove handle, place the detector
block in the instrument and connect the detector cable,
remove the transport locking screws, connect the leak sensor
cable and connect the drainage tubing.
In continuation, set up the waste collector by assembling the
cap and screwing it onto the vessel. Then hang the waste
collector holder on the side of the IC, so you can observe the
droplets coming out of the capillaries later connected to the
collector. Attach the waste tube to the vessel and lead it to
the waste canister.When the tube is too long, please shorten
it, because it is important to have a high level difference for
the liquid to drain.
The power cable and USB cable (6.2151.020) are plugged
into the rear of the Professional IC Vario. Please don’t switch
on the instrument yet. This step will follow after the completed
installation.
2.3. Accessory Kit: Vario/Flex ONE (6.5000.010)
In the box with the Accessory Kits ONE, you will find all the
accessories for setting up the Eluent bottle. Please lead the
aspiration tube for the Eluent through the M8 stopper, the
earring and the eluent cap. Then fix the white weight
(6.2744.210), the adaptor (6.2744.210) and the aspiration
filter (6.2821.090) on the eluent aspiration tube, all the while
being careful not to touch the filter and its connections with
bare hands in order to avoid cross contamination. Also fix the
filled adsorbertube on the eluentcap. Please refer to the 940
Professional IC Vario manual for a detailed description.
2.4. 858 Professional Sample Processor
For a detailed description, please refer to the 858
Professional Sample Processor manual. In general, you will
have to do the following: Plug in the Swing Head connection
Nr Article no. Article designation
IC
1 2.940.1500 Professional IC Vario ONE/SeS/PP
Detector
1
2.850.9010
IC Conductivity Detector
Sample processor
1 2.858.0020 Professional Sample Processor -
Pump
Accessories
1 2.741.0010 741 Magnetic Stirrer
1 2.800.0010 800 Dosino
1 6.5330.120 IC Equipment: Inline Dilution
1
6.5330.110
IC Equipment: Inline Ultrafiltration
Optional
1 6.6059.302 MagIC Net 3.0 Professional CD –
1 license
1
6.6059.303
MagIC Net 3.0 Multi – 3 licenses
1 6.2041.760 Sample rack 54 x 11 mL +
1 x 300 mL
1 6.2041.440 Sample rack 148 x 11 mL +
3 x 300 mL
1 6.2743.050 Sample tubes 11 mL
1 6.2743.070 Stopper with perforation
1
6.1030.420
Metrosep A Supp 15 - 150/4.0
1
6.1030.500
Metrosep A Supp 15 Guard/4.0
1
6.1052.420
Metrosep C Supp 1 – 150/4.0
1
6.1052.500
Metrosep C Supp 1 Guard/4.0
1 6.2842.200 MSM-HC Rotor C
1 6.2832.000 MSM Rotor A
1 6.2842.000 MSM-HC Rotor A
1 6.2844.000 MSM-LC Rotor A
1
6.2842.020
Adapter Vario to MSM and MSM-LC
1
2.941.0010
Eluent Production Module
1
2.800.0010
800 Dosino
1
6.3032.150
Dosing Unit 5 mL
1 6.2744.080 M6 thread / UNF 10/32 coupling

Application Bulletin 376
Installation Instruction for ProfIC Vario 6 Anion
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cable and the power supply cable. The controller cable
(6.2151.000) is plugged into the plug “Contr.” on the 858 and
connected to the IC device via USB. The sample processor
initializes and lifts its Swing Head, once the IC device it is
connected to is recognizedby the software. For this, you have
to plug the USB cable of the IC into the PC and turn it on.
Afterwards, it is possible to mount the retaining plate, the
needle and the safety shield (for detailed instructions please
refer to the 858 Professional Sample Processor manual).
Please remove one element of the energy chain close to the
swing head by using a screwdriver to unfix it. In its place,
connect the transfer tubing with holder 2 x M6 / 10 mL
(6.1562.130). Then connect one end of the transfer tubing
onto the swing arm, where the aspiration tip is connected with
the help of the adapter UNF 10/32 outer / M6 inner
(6.2744.200).
For the following installations, please shut down the IC again
and disconnect the 858 from the power supply.
2.4.1. Liquid Handling Station (LQH Station)
The Liquid Handling Station consists of two function units:
The rinsing unit is equipped with a two-pipe system and is
used for rinsing the sample tube from the inside and outside.
The secondunit is used for dilutionof samplesand standards.
In the present case, both units will be used.
Before adding the Liquid Handling Station onto the Sample
Processor insert the stirrer into the socket of the LQH Station.
This is done according to the description in the Liquid
Handling Station manual (chapter 2). Subsequently, it is
mounted onto the left side of the Sample Processor. For
installation instructions, please refer to the Liquid Handling
Station manual. All parts are included in the IC Equipment:
Inline Dilution (6.5330.120). Please make sure that the Liquid
Handling Station (LQH Station) is aligned properly before
continuing the setup. All angle settings will be configured in a
later step.
2.4.1.1. Rinsing unit
The waste tubing (6.1801.120) is fixed at the bottom of the
rinsing station to drain the waste water.
Ultra pure water for rinsing will be supplied by the dilution
Dosino. Please fill the 2 Liter bottle with ultra pure water. The
water will be needed for dilution and rinsing purpose and the
installation of the Dosino and its connecting parts will be
mentioned in the next paragraph.
Please close the hole at bottom of the LQH Station (No.2 in
the image) with a stopper.
2.4.1.2. Dilution unit and 800 Dosino
At port 1 of the 10 mL Dosing Unit, please connect the loose
end of the transfer tubing (6.1841.000). Port 2 is used for
ultrapure water supply; therefore please screw the FEP
aspiration tubing (6.1819.110) into Port2 on the bottom of the
Dosing Unit. Then fix the Dosing Unit onto the 2 liter bottle
with ultra pure water. Adjust the Dosino on top and connect
its cable on the backside of the 858 Sample Processor on
MSB1. Please make sure that the 858 is switched off!
The stirrer cable is plugged in at the tower into the -
socket. Capillary connections are carried out according to the
following scheme.
1. Transfer Tubing with holder 2 x M6 / 10 mL
(6.1562.130) to be connected to Dosino port 1. The
holder can be fixed as a chain element at the Tower.
The other end is fixed to Adapter UNF 10/32 outer /
M6 inner (6.2744.200) (see 2.)

Application Bulletin 376
Installation Instruction for ProfIC Vario 6 Anion
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2. Adapter UNF10/32 outer / M6 inner (6.2744.200) for
the connection between the needle and the transfer
tubing
3. PTFE capillary 0.97 mm i.d. / 40 cm (6.1803.070)
connected to the grey/grey pump tubing with a
coupling nozzle (6.2744.034). Its other end is
connected to position 1 on the side of the LQH
Station with a PEEK pressure screw (6.2744.010)
4. Pump tubing LFL (gray/gray), 3 stoppers
(6.1826.380)
5. The PTFE capillary 0.97 mm i.d. / 1 m (6.1803.080)
is connected to the IC valve position 1. The other
end is attached to the gray/gray pump tubing with
the help of a nozzle coupling with security device
(6.2744.160)
2.4.2. Ultrafiltration cell
In this setup,an ultrafiltration cell is mounted after the dilution
of the sample (side opening of Liquid Handling Station) and
before the IC system. For this, please mount the cell holder
which is included in the IC Equipment: Ultrafiltration
(6.5330.110) onto the Sample Processor on the right side of
the tower. For detailed instructions, please refer to the
Ultrafiltration Cell Manual.
The filter membrane is soaked in ultrapure water for
preconditioning. After inserting the filter membrane and the
sealing ring, the cell is tightly screwed together.
Capillaryconnections are shown in the illustration below. The
flow must be higher at the sample side than at the filtrate side.
That is why it is important to use a bigger pump tubing
(gray/gray) and 0.97 mm i.D. capillaries on the sample side,
and thinner pump tubing (yellow/orange) and the shortest
possible 0.5 mm i.D. capillaries on the filtrate side. Only use
the PVDF screws in combination with the UF cell as the
material of the cell is prone to break with PEEK screws.
1. PTFE capillary 0.97 mm i.d. / 40 cm (6.1803.070)
connected to the side of the Liquid Handling Station
(Nr. 4 of chapter 2.4.1.1)
2. PEEK pressure screw (6.2744.070)
3. Coupling olive (6.2744.030)
4. Pump tubing (6.1826.380) with gray-gray stoppers
for conveying the sample
5. PTFE capillary (6.1803.060) for conveying the
sample from the peristaltic pump to the ultrafiltration
cell (0.97 mm i.D.)
6. PVDF pressure screw (6.2744.000) for connecting
the capillaries to the ultrafiltration cell
7. PTFE capillary (6.1803.080) for conveying the
sample from the ultrafiltration cell to the waste
collector (0.97 mm i.D.)
8. PTFE capillary (6.1803.050) for conveying the
filtrate from the ultrafiltration cell to the peristaltic
pump
9. PEEK capillary (6.1831.060) for conveying the
filtrate from the peristaltic pump to the injection valve
for anion analysis in the IC instrument
10. Pump tubing (6.1826.320) with yellow-orange
stoppers for conveying the filtrate
11. Pump tubing connection with safety device
(6.2744.160)
2.5. Interconnection of devices
The whole setup of the ProfIC Vario 6 Anion packet is
depicted here:

Application Bulletin 376
Installation Instruction for ProfIC Vario 6 Anion
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Prepare and degas an eluent suitable for the column you
want to use (see column manual).
Provide the regeneration solution for the MSM Suppressor
(100 mmol/L sulfuric acid for anions or 70 mmol/L Na2CO3,
70 mmol/L NaHCO3 for cations).
2.6. 940 Professional IC Vario
Capillaries are connected according to the following list and
the diagram below:
1. Connection to the eluent bottle
2. Capillary to Column inlet – 0.25 mm ID
3. MSM inlet capillary – labeled with in
4. MSM outlet capillary – labeled with out
can be connected to the MCS or directly to the
detector using a coupling (6.2744.040)
5. Detector inlet capillary
6. Detector outlet capillary connected to the MSM
inlet capillary – labeled with rinsing solution with
the help of a coupling (6.2744.040)
7. Regeneration solution aspiration capillary – PTFE,
0.5 mm ID, connected to the bottle with 100 mmol/L
sulfuric acid and the orange/yellow pump tubing (8).
This pump tubing is, on the other side, relied to the
regeneration solution capillary of the MSM - labeled
with regenerant - with a security lock and filter (19)
8. Peristaltic pump tubing with orange/yellow stoppers
(6.1826.420)
9. MSM regeneration inlet capillary – labeled with
regenerant
10. MSM regeneration solution outlet capillary – labeled
with waste reg. – to be connected to the waste
collector
11. MSM rinsing solution outlet capillary – labeled with
waste rins. - to be connected to the waste collector
12. MSM rinsing solution inlet capillary -labeled with
rinsing solution– connected to the detector outlet
capillary with a coupling (6.2744.040)
13. MCS air aspiration capillary – connected to the CO2-
adsorber cartridge
14. Sample aspiration capillary – PEEK, 0.97 mm ID,
coming from the Ultrafiltration Cell (chapter 2.4.2,
number 9)
15. Sample outlet capillary – PTFE, 0.97 mm ID, to be
placed into the waste collector
16. PEEK pressure screw short (6.2744.070)
17. Coupling 2xUNF 10/32 PEEK (6.2744.040)
18. Coupling nozzle - UNF 10/32 (6.2744.034)
19. Pump tubing connector with security lock and filter
(6.2744.180)
20. PEEK pressure screw long (6.2744.090)
21. Luer coupling – connect with short PEEK pressure
screw to CO2-adsorber cartridge
22. The UNF 10/32 coupling (6.2744.040) is installed
instead of the column to rinse the system with
eluent. After rinsing the column is installed.
The sample injection is at position 1 of the valve. A counter
current flow of the sample with regard to the eluent flow is
recommended in order to minimize diffusion and carry over.
Make sure that all outlet capillaries are put into the waste
collector.
3. MagIC Net
3.1 Configuration
Please adjust the “view layout” of the configuration. Please
configure it in the following way (the order of the sub windows
is up to you):

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Installation Instruction for ProfIC Vario 6 Anion
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Now please connect the USB cables from the instruments
and turn their power on. Connected USB devices are
automatically recognized when MagIC Net is started. After
confirmation of the automatically generated requests, the
devices and columns are stored in the configuration. The
devices are predefined as “940 Professional IC Vario 1” and
“858 Professional Sample Processor 1”. Name them
accordingly, if other names appear in your configuration (e.g.
due to changed settings on your computer).
Accept the request to add the Dosino connected to the 940,
name the Dosino solution “UPW” (for ultra pure water) and
chose “Dosino” in the “Use” dropdown menu.
In the window configuration, the 940 Professional IC Vario,
the 858 Professional Sample Processor and the column are
visible. Add and define the eluent, the suppressor solution
and the MSM Rotor.
The settings for the rack require a “work position” fitting to the
respective rack (e.g. 125 mm for rack 6.2041.760).
For using the Liquid Handling Station, it is necessary to define
external positions for the needle. This is done in the
configuration of the Sample Processor in the subtab “Tower”.
Please define the external positions 1, 2 and 3 according to
the picture below. Attention: The angles are only approximate
values, for fine tuning, please use the manual control to find
the correct values for this system and adjust them then in the
configuration.
For the Dosino, the exact lengths and diameters of the
tubings used need to be added for it to work properly. This
can be done in the configuration as follows:
Please add a common variable called: “end volume” and set
its value to 9 mL.
3.2 Method adjustment
The method for this configuration can be found in the
example method folder on your MagIC Net - CD. In the
window method, import the method for ProfIC Vario 6 Anion
from the installation CD: Go to File → method manager and
choose your method group. Afterwards, click on edit → import

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Installation Instruction for ProfIC Vario 6 Anion
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and choose the pathway on the installation CD:
MagICNet\examples\methods\ ProfIC Vario \ProfIC
Vario_6_Anion.imet
In the window method, open the ProfIC Vario 6 Anion method
and assign the following equipment: Assign the suppressor
solution to the IC peristaltic pump and set the speed rate to
1, add your Rotor to the MSM and activate the automatic
stepping all 10 minutes, define the rack of the connected
sample processor, assign the eluent to the IC pump and
assign the column to the analysis. Adjust the temperature of
the column oven to 45°C. Check the analytes in the
components table and adapt if necessary.
Perform a method test and save the method.
3.3. Purge of the system
Before inserting the column, disconnect the Out capillary of
the suppressor connection piece from the MCS or from the
detectorand put it into awastebeaker.Thesuppressorneeds
to be rinsed with the system first and its waste should not
pass through the sensitive MCS and/or detector in the
beginning, as loose particles could be flushed out.
Now, please flush the system for about 10 minutes and get
rid of air bubbles (by using the purge valve and syringe).
During these 10 minutes, step the MSM three times in order
to flush all three chambers.
As soon as the whole system is purged, reconnect the
suppressor Out capillary back to the MCS or the detector.
Now insert and rinse the precolumn for 10 minutes by leading
the outlet directly into the waste. Afterwards connect the
analytical column and flush it likewise for 10 minutes. Only
afterward the columnis completelyconnected to theflow path
of the eluent (see also 940 manual). Start the peristalticpump
and adjust the pressure of the lever on the tubing, in order to
see the droplets of suppressor regeneration solution drop into
the waste collector. Rinse the Dosino and fill the transfer
tubing by applying the “prepare” button under manual control.
In order tostart the equilibration, goto the windowwork place,
load the anion method, and press “Start HW”.
Equilibrate the system until the baseline is stable.
3.4. User defined determination
In the window method, under evaluation, enter the ions and
the concentrations of the required standards. You can either
calibrate with a set of calibration standards, or you can
calibrate with only one standard and diluting this one to
different calibration levels.
If you wish to calibrate by diluting one standard solution, the
standard with the highest concentration has to be prepared
and labeled as standard 1 in this method. All other standards
have to be divisors of standard 1, e.g. all components of
standard 2 have half the concentration of those in standard
1, all components of standard10 have a tenth of the standard
1 concentration.
Always use the standard number as the factor that lies
between the two solutions. Make sure to always fix the
dilution factor = 1 for all the standards in your determination
table.
Now please add the correct Database in the Evaluation
window (Evaluation - Results - Database) for the method.
In the window work place, set up a “determination series”,
describing your samples by ident, vial number, sample type
(standard, blank, or sample etc.). After putting the analyte
solutions onto the rack, press “start”.
In the field dilution, indicate the dilution you wish to apply to
your sample, e.g “2”if you wantto dilute your sample 2times.
If no dilution is required or a standard is measured, write 1.
After putting the analyte solutions onto the rack, press “start”.
For evaluation and after recording the first chromatogram,
check the retention times of your compounds. Since they
depend on the performance ofyour column, you may have to
adjust them in your method.

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Installation Instruction for ProfIC Vario 6 Anion
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4. Exemplary measurement
This data was recorded using a MSM Rotor A with the
Adapter Vario on a Metrosep A Supp 15 - 150/4.0 column.
Fluoride, chloride, nitrite, bromide nitrate, sulfate, and
phosphate were measured as exemplary standard anions
(5 ppm). With Metrosep A Supp 15 - 150/4.0, sulfate elutes
faster than phosphate.
For the calibration, the average peak areas of 3 injections
were evaluated. Within the concentration range of 1 –
20 ppm, a quadratic curve type was used for evaluation.
Correlation coefficients and standard deviations are listed in
the following table. The calibration curve for fluoride is shown
as an example.
Correlation
coefficient Percentage standard deviation
[%]
Fluoride 0.999993 0.414
Chloride 0.999979 0.739
Nitrite
0.999938
1.279
Bromide
0.999936
1.316
Nitrate
0.999942
1.254
Sulfate
0.999967
0.946
Phosphate 0.999974 0.845
The precision of 10 injections of a 2 ppm solution of standard
anions was determined. The results are shown in the
following table and an exemplary trend chart is shown for
phosphate.
Ion
Standard deviation
relative [%] absolute [ppm]
Fluoride 0.005 0.264
Chloride 0.013 0.652
Nitrite 0.014 0.709
Bromide
0.008
0.387
Nitrate
0.012
0.569
Sulfate
0.001
0.052
Phosphate
0.004
0.168

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Installation Instruction for ProfIC Vario 6 Anion
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5. Optional equipment
5.1. Eluent Production Module
The 941 Eluent Production Module creates fresh new eluent
out of eluent concentrate and ultrapure water. For installation
instructions and further information, please refer to the 941
Eluent Production Module Manual.
5.2. Alternative MSM rinsing and regeneration
methods
For alternative suppressor rinsing and regeneration methods
and setups, please refer to the AB-395.