Metrohm 761 SD Compact IC User manual

761 SD Compact IC
8.761.1043
Instructions for Use
CH-9101 Herisau/Schweiz
E-Mail [email protected]
Internet www.metrohm.com

CH-9101 Herisau/Schweiz
E-Mail [email protected]
Internet www.metrohm.com
761 SD Compact IC
8.761.1043 Instructions for Use
8.761.1043 02.2004 / chs
POWER
761 SD Compact IC

Teachware
Metrohm AG
Oberdorfstrasse 68
CH-9101 Herisau
[email protected]
1. Edition 2004
These instructions are protected by copyright. All rights reserved.
Although all the information given in these instructions has been checked with great care, errors
cannot be entirely excluded. Should you notice any mistakes please inform the author at the
address given above.

Table of contents
761 SD Compact IC / Instructions for Use 8.761.1043
I
Table of contents
1Introduction ................................................................ 1
1.1 Instrument description ................................................................................1
1.2 Parts and controls .......................................................................................3
1.2.1 Front view ........................................................................................................... 3
1.2.2 Rear view ............................................................................................................ 4
1.2.3 Connection schematic ....................................................................................... 5
1.3 Information on the Instructions for Use .....................................................7
1.3.1 Organisation ....................................................................................................... 7
1.3.2 Notation and pictograms.................................................................................... 8
1.4 Safety notes .................................................................................................9
1.4.1 Electrical safety................................................................................................... 9
1.4.2 General precautionary rules ............................................................................... 9
2Installation ............................................................... 10
2.1 Flow chart ..................................................................................................10
2.2 Setting up the instrument..........................................................................11
2.2.1 Packaging......................................................................................................... 11
2.2.2 Check................................................................................................................ 11
2.2.3 Location ............................................................................................................ 11
2.3 Description of the connections.................................................................12
2.3.1 Connection of capillaries/tubing ...................................................................... 12
2.3.2 Connection between capillaries/tubing............................................................ 13
2.3.3 Filter unit PEEK ................................................................................................. 14
2.4 Connection of the detector block .............................................................15
2.5 Installation of the MPak cabinet and connection of the drain tubes ......16
2.5.1 Installing the MPak cabinet .............................................................................. 16
2.5.2 Drain tube for inner compartment.................................................................... 16
2.5.3 Drain tube for MPak cabinet............................................................................. 16
2.6 Installing the eluent path ..........................................................................17
2.6.1 High-pressure pump – Removing the transport security screws .................. 17
2.6.2 Connection MPak →high-pressure pump ...................................................... 17
2.6.3 Connection high-pressure pump →injection valve ........................................ 18
2.6.4 Connection injection valve →suppressor ....................................................... 19
2.6.5 Connection suppressor →detector................................................................. 19
2.6.6 Connection detector →suppressor................................................................. 19
2.6.7 Connection suppressor →waste..................................................................... 20
2.7 Installing the regenerant path...................................................................20
2.7.1 Fitting the pump tubing for regenerant ............................................................ 20
2.7.2 Connection regenerant-MPak →pump tubing →suppressor →waste ........ 21
2.8 Installing the sample path.........................................................................23
2.8.1 Fitting the pump tubing for sample.................................................................. 23
2.8.2 Connection sample vessel →pump tubing →injection valve →waste ........ 24
2.9 Connecting the 766 IC Sample Processor ...............................................26
2.9.1 Installing the 766 IC Sample Processor ........................................................... 26
2.9.2 Connecting the 766 IC Sample Processor....................................................... 26
2.10 Fitting the rear panel .................................................................................27
2.11 Mains connections ....................................................................................28
2.11.1 Setting the mains voltage................................................................................. 28
2.11.2 Fuses ................................................................................................................ 29
2.11.3 Mains cable and mains connection ................................................................. 29
2.11.4 Switching the instruments on/off...................................................................... 29

Table of contents
761 SD Compact IC / Instructions for Use 8.761.1043
II
2.12 Connection to the PC ................................................................................30
2.12.1 Connecting cable 6.2134.100.......................................................................... 30
2.12.2 Software installation ......................................................................................... 30
2.12.3 Basic settings «IC Net»..................................................................................... 31
2.12.4 Basic settings «IC Cap» ................................................................................... 33
2.13 Deaerating the pump and rinsing the pulsation dampener ....................34
2.13.1 Deaerating the pump ....................................................................................... 34
2.13.2 Rinsing the pulsation dampener...................................................................... 35
2.14 Rinsing before fitting the column .............................................................37
2.15 Precolumn and separating column ..........................................................39
2.15.1 Metrosep RP Guard ......................................................................................... 39
2.15.2 Metrosep A Supp 1 HS separating column..................................................... 40
2.16 Attaching tubing to side panels................................................................42
3«IC Net» ..................................................................... 43
3.1 «IC Net» – User interface for the 761 SD Compact IC..............................43
3.1.1 Systems - Methods .......................................................................................... 43
3.1.2 Opening a system ............................................................................................ 43
3.1.3 Opening a method ........................................................................................... 44
3.1.4 Connect a system ............................................................................................ 44
3.1.5 Instrument icon................................................................................................. 45
3.1.6 System parameters for linked system ............................................................. 45
3.1.7 Hardware settings ............................................................................................ 50
3.2 Systems supplied ......................................................................................54
3.2.1 System "startup.smt" ........................................................................................ 54
3.2.2 System "manual.smt"........................................................................................ 55
3.2.3 System "auto.smt" ............................................................................................ 56
3.2.4 System "shutdown.smt".................................................................................... 61
4«IC Cap» .................................................................... 64
4.1 «IC Cap» introduction ................................................................................64
4.1.1 Login................................................................................................................. 64
4.1.2 User interface ................................................................................................... 64
4.2 «IC Cap» - Configuration ...........................................................................66
4.2.1 Predefined configurations ................................................................................ 66
4.2.2 Configuration "manual.cfg"............................................................................... 66
4.2.3 Configuration "auto.cfg" ................................................................................... 72
5Operation .................................................................. 77
5.1 Operation with manual sample change....................................................77
5.1.1 Control with «IC Net» (manual operation) ........................................................ 77
5.1.2 Control with «IC Cap» (manual operation) ....................................................... 81
5.2 Operation with automated sample change ..............................................84
5.2.1 Control with «IC Net» (automated operation)................................................... 84
5.2.2 Control with «IC Cap» (automated operation) ................................................. 86
6Notes – Maintenance – Faults .................................. 88
6.1 Practical notes on ion chromatography ...................................................88
6.1.1 Separating columns ......................................................................................... 88
6.1.2 High-pressure pump ........................................................................................ 88
6.1.3 Eluents.............................................................................................................. 89
6.1.4 Peristaltic pump................................................................................................ 89
6.1.5 Suppressor module.......................................................................................... 89
6.1.6 Connections ..................................................................................................... 90

Table of contents
761 SD Compact IC / Instructions for Use 8.761.1043
III
6.2 Maintenance and servicing .......................................................................91
6.2.1 General information .......................................................................................... 91
6.2.2 Passivation........................................................................................................ 91
6.2.3 Shutdown.......................................................................................................... 91
6.2.4 Changing separating columns......................................................................... 92
6.2.5 Maintenance work on the pump head ............................................................. 92
6.2.6 Regeneration of the suppressor module ......................................................... 98
6.2.7 Cleaning the suppressor ................................................................................ 100
6.2.8 Replacing the suppressor .............................................................................. 102
6.2.9 Replacing the pump tubing............................................................................ 103
6.3 Faults and malfunctions..........................................................................104
6.3.1 Error messages .............................................................................................. 104
6.3.2 Malfunctions and their rectification ................................................................ 104
6.4 Diagnostic tests / Validation / GLP.........................................................106
7Appendix ................................................................. 107
7.1 Technical data .........................................................................................107
7.1.1 Conductivity measurement ............................................................................ 107
7.1.2 Conductivity detector ..................................................................................... 107
7.1.3 Injection valve ................................................................................................. 108
7.1.4 High-pressure pump ...................................................................................... 108
7.1.5 Peristaltic pump.............................................................................................. 109
7.1.6 Suppressor module........................................................................................ 109
7.1.7 Leak detector.................................................................................................. 109
7.1.8 RS232 interface .............................................................................................. 109
7.1.9 Remote interface ............................................................................................ 110
7.1.10 Mains connection ........................................................................................... 110
7.1.11 Safety specifications....................................................................................... 111
7.1.12 Electromagnetic compatibility (EMC)............................................................. 111
7.1.13 Ambient temperature...................................................................................... 111
7.1.14 Housing .......................................................................................................... 111
7.2 Standard equipment................................................................................112
7.3 Optional accessories ..............................................................................118
7.3.1 6.5328.000 SD Spare Part Set ....................................................................... 118
7.3.2 Other optional accessories ............................................................................ 120
7.4 Warranty and conformity.........................................................................121
7.4.1 Warranty.......................................................................................................... 121
7.4.2 Declaration of Conformity............................................................................... 122
7.4.3 Quality Management Principles ..................................................................... 123
7.5 Index.........................................................................................................124

Table of contents
761 SD Compact IC / Instructions for Use 8.761.1043
IV
List of figures
Figure 1: Front of the 761 SD Compact IC ............................................................... 3
Figure 2: Rear panel 761 SD Compact IC ................................................................ 4
Figure 3: Connection schematic for 761 SD Compact IC ........................................ 5
Figure 4: Connectors for capillaries........................................................................ 13
Figure 5: PEEK couplings....................................................................................... 13
Figure 6: Connecting the filter unit PEEK ............................................................... 14
Figure 7: Position of the detector block 40 ............................................................ 15
Figure 8: Pump tubing for regenerant path ............................................................ 21
Figure 9: Flow schematic regenerant flow.............................................................. 22
Figure 10: Pump tubing for sample path.................................................................. 24
Figure 11: Flow schematic sample stream............................................................... 25
Figure 12: Rear panel ............................................................................................... 27
Figure 13: Setting the mains voltage........................................................................ 29
Figure 14: Connection of precolumn and separating column.................................. 41
Figure 15: Components of the pump head .............................................................. 94
Figure 16: Replacement of the piston seal 76.......................................................... 94
Figure 17: Components of inlet valve 77 and outlet valve 78 .................................. 97
Figure 18: Assembling the suppressor .................................................................. 101

1.1 Instrument description
761 SD Compact IC / Instructions for Use 8.761.1043
1
1 Introduction
1.1 Instrument description
The 761 SD Compact IC is a version of the time-proven 761 Compact
IC which has been developed specifically for soft drink analysis. The
761 SD Compact IC is designed for determining phosphoric acid and
its intermediate products in soft drinks but also continues to offer all
functionalities of the original version.
The advantages of the 761 SD Compact IC over the conventional
methods of phosphoric acid analysis in soft drinks are its short analysis
times, its ease of operation and the option for automated sampling.
There is no need for time-consuming sample preparation and only
small quantities of chemicals are consumed (more environmentally
friendly).
The 761 SD Compact IC has a very compact housing accommodating
all equipment required for ion-chromatographic determination:
• Injection valve – this supplies the sample to the eluent stream by
switching over the flow paths.
• High-pressure pump – extremely low-pulsation dual piston pump
with a flow range of 0.2 to 2.5 mL/min and a maximum pressure
of 25 MPa (250 bar).
• Pulsation dampener – the pulsation dampener protects the sepa-
rating column reliably against damage even in the case of low-level
pressure fluctuations.
• Column chamber – perfect insulation of the housing not only cre-
ates thermally stable conditions for the separating column, but also
shields the system against electromagnetic interference.
• Column – Metrosep A Supp 1 HS anion column as IC separating
column; Metrosep RP Guard as protective precolumn.
• Suppressor – the Metrohm-Suppressor-Module (MSM) which is
already integrated features stable pressure characteristics, auto-
matic regeneration, high performance and optimum reproducibility.
• Peristaltic pump – integrated, two-channel peristaltic pump with a
flow rate of 0.5 to 0.6 mL/min for regeneration of the suppressor
module and aspiration of the sample for manual operation.
• Detector – conductivity detector with excellent temperature stabil-
ity. The detector temperature fluctuates by less than 0.01°C and
can be matched optimally to ambient conditions.

1Introduction
761 SD Compact IC / Instructions for Use 8.761.1043
2
All components coming into contact with eluent and sample are metal-
free. Ready-to-use eluents in certified MPaks are supplied for soft drink
analysis.
The 761 SD Compact IC is operated by means of a PC connected to
the RS232 interface. The 761 SD Compact IC can be controlled by the
«IC Net» software or by the “master” «IC Cap» software.
The systems required for soft drink analysis (and the methods linked to
them) are supplied on the installation CD.
The following instrument versions are available:
• 2.761.0420 761 SD Compact IC
• 2.761.0520 761 SD Compact IC with IC Sample Processor 766
for automated sample change
Principle:
The eluent flows on the eluent path through the injection valve into the
separating column and then into the detector. The sample flows on the
sample path also through the injection valve and the red sample loop
visible at the centre of the injection valve. At a specific instant (<In-
ject>; controlled by the program), the flow paths in the injection valve
are switched and the sample loop is reintegrated in the eluent path (it is
now no longer a part of the sample path). The sample quantity which is
located in the sample loop at the switchover instant is then entrained
with the eluent stream, thus entering the separating column (where the
actual chromatographic separation of the components occurs) and, fi-
nally, chromatographically separated, into the conductivity detector.

1.2 Parts and controls
761 SD Compact IC / Instructions for Use 8.761.1043
3
1.2 Parts and controls
In this section you will find the numbers and designations of the parts
and controls of the 761 SD Compact IC. The numbering applies
throughout the instructions for use, i.e. bold numbers in the text (e.g.
4 ) refer to the parts and controls illustrated here.
1.2.1 Front view
Figure 1: Front of the 761 SD Compact IC
1Door to inner compartment
2Feedthrough for aspirating tubing
3Aspirating tubing
for sample
4Feedthrough for capillaries
5Connection for drain tube
for discharging escaped fluid from the
inner compartment
6Connection purge valve
7Pilot lamp
This is on when the instrument is
switched on
POWER
761 SD Compact IC
1
2
3
4
7
6
5

1Introduction
761 SD Compact IC / Instructions for Use 8.761.1043
4
1.2.2 Rear view
Figure 2: Rear panel 761 SD Compact IC
8Opening for outlet capillaries
for discharge of eluent, regeneration
solution and sample solution
9Opening for inlet capillaries
for supply of eluent, regeneration solu-
tion and sample solution
10 Opening for detector cable
11 Knurled screw
for fastening the rear panel
12 Detachable rear panel
Access to the inner compartment
13 Transport security screws
to secure the pump head when the in-
strument is transported
14 Mains switch
to switch instrument on and off:
I = ON 0 = OFF
15 Mains connection plug
Mains connection, see section 2.11
16 Fuse holder
Changing the fuses, see section 2.11.2
17 Serial number
18 Analogue output
output for analogue signal
19 Remote interface
Remote-I/O lines for connection of ex-
ternal devices
20 Connection for detector block
21 RS232 interface
PC connection
WARNING - Fire Hazard -
with the same type and rating of fuse
For continued protection replace only
Type 1.761.
Fuse
100-120V:
220-240V:
1,0A(T)
0,5A(T)
Made by Metrohm Herisau Switzerland
RS 232
Detector Block
Remote
Waste B Waste A
Transport security screws
Analog Output
8
9
10
11 11
13
14
15
16
21
20
19
18
17
12

1.2 Parts and controls
761 SD Compact IC / Instructions for Use 8.761.1043
5
1.2.3 Connection schematic
Figure 3: Connection schematic for 761 SD Compact IC
3 Aspirating tubing
for sample
22 MPak cabinet
for suspending eluent and regener-
ant bag
23 Tubing connection to MPak
6.1837.000
24 Pump head 6.2824.100
25 Connection capillary
Connection between pump head and
purge valve, permanently mounted
26 Purge valve
27 Connection capillary
PEEK capillary 6.1831.010 for
deaerating,
length L= 15 cm
28 Connection capillary
PEEK capillary 6.1831.010,
length L= 13 cm
29 Connection capillary
PEEK capillary 6.1831.010,
length L= 13 cm
30 Pulsation dampener 6.2620.150
31 Inlet capillary for injector
PEEK capillary 6.1831.010,
length L= 24 cm
3
29
30
48
22
23
55
24
31 32 33
34 36
35 37 38
54
56
42
44
23
55 61 56
45
46
49
48
25
26
28 56
27
39
41
64
63 65
50
50
43
47
40

1Introduction
761 SD Compact IC / Instructions for Use 8.761.1043
6
32 Sample loop 1.5 µL (6.1825.240)
PEEK sample loop
33 Column connection capillary
PEEK capillary 6.1831.010,
length L= 30 cm
34 Metrosep RP Guard 6.1011.020
Precolumn for protecting the separat-
ing column
35 Connection capillary
PEEK connection capillary between
precolumn and separating column
36 Metrosep A Supp 1 HS separating
column (6.1005.350)
IC separating column
37 Suppressor inlet capillary for elu-
ent ("Eluent")
PTFE capillary, permanently mounted
on suppressor, labelled "Eluent"
38 Suppressor outlet capillary for
eluent ("Detector")
PTFE capillary permanently mounted
on suppressor, labelled "Detector"
39 Inlet capillary to detector block
PEEK capillary, permanently
mounted
40 Detector block 1.732.0420
41 Outlet capillary from detector
block
PEEK capillary, permanently
mounted
42 Suppressor inlet capillary for elu-
ent ("H2O")
PTFE capillary, permanently mounted
on suppressor, labelled "H2O"
43 Suppressor module
44 Suppressor outlet capillary for
eluent ("Waste")
PTFE capillary, permanently mounted
on suppressor, leading to the waste,
labelled "Waste"
45 Suppressor inlet capillary for re-
generant
PTFE capillary, permanently mounted
on suppressor, labelled "H2SO4"
46 Suppressor outlet capillary for
regenerant ("Waste")
PTFE capillary, permanently mounted
on suppressor, leading into the
waste, labelled "Waste"
47 Connection capillary
PTFE capillary 6.1803.030, connec-
tion between pump tubing and injec-
tion valve
48 Connection capillary
PTFE capillary 6.1803.030, connec-
tion between injection valve and
waste container
49 Pump drive of the peristaltic
pump
Drive of the two-channel-peristaltic
pump for pumping sample and re-
generant
50 Pressure screw 6.2744.010
54 PEEK coupling 6.2744.040
55 PEEK coupling 4.455.4500
Coupling for "tubing connection to
MPak"
56 Filter unit PEEK 6.2821.120
61 Pump tubing 6.1826.110 for H2SO4
63 PEEK coupling 6.2744.030
64 Pump tubing 6.1826.110 for sam-
ple
65 PEEK coupling 6.2744.160
with tubing security device
On instrument version 2.761.0520 with automated sample change
with the 766 IC Sample Processor, the aspirating tubing
3 is replaced
by the PEEK capillary tubing
18 (see Instructions for Use 766 IC Sam-
ple Processor) installed on the 766 IC Sample Processor. See Section
2.9. for installation of the 766 IC Sample Processor.

1.3 Information on the Instructions for Use
761 SD Compact IC / Instructions for Use 8.761.1043
7
1.3 Information on the Instructions for Use
Please read through these Instructions for Use carefully before you put
the 761 SD IC Compact IC into operation. The Instructions for Use
contain information and warnings to which the user must pay attention
in order to assure safe operation of the instrument.
1.3.1 Organisation
These Instructions for Use 8.761.1043 for the 761 SD Compact IC
provide a comprehensive overview of installation, startup procedure,
operation, fault rectification and technical specifications of this instru-
ment. The Instructions for Use are organised as follows:
Section 1 Introduction
General description of instrument, parts and con-
trols and safety notes
Section 2 Installation
Installation and connection of the instrument, of the ac-
cessories and of the software
Section 3 «IC Net»
Explanatory information on the user interface of the «IC
Net» control software
Section 4 «IC Cap
Explanatory information on the user interface
of the «IC Cap» control software
Section 5 Operation
Description of operation with manual and
automatic sample change, with «IC Net» and
«IC Cap» in each case
Section 6 Notes – Maintenance – Faults
Notes on ion chromatography, maintenance, fault
rectification, diagnostic tests and validation
Section 7 Appendix
Technical data, standard equipment, options, war-
ranty, declarations of conformity and index
To find the required information on the instruments, you will find it an
advantage to use either the Table of contents or the Index at the
back.

1Introduction
761 SD Compact IC / Instructions for Use 8.761.1043
8
1.3.2 Notation and pictograms
The following notations and pictograms (symbols) are used in these In-
structions for Use:
Fill Menu item, parameter or entry
value
in the software
SYSTEM STATE Program window
in the software
<OK> Button
in the software
20 Part or control of 761 SD
18 Part or control of 766
Hazard
This symbol draws attention to a
possible danger to life or of injury if
the associated directions are not
followed correctly. .
Warning
This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed cor-
rectly.
Caution
This symbol marks important in-
formation. First read the associated
directions before you continue.
Comment
This symbol marks additional in-
formation and tips.

1.4 Safety notes
761 SD Compact IC / Instructions for Use 8.761.1043
9
1.4 Safety notes
1.4.1 Electrical safety
While electrical safety in the handling of the 761 SD Compact IC is as-
sured in the context of the specifications IEC/EN 61010-1 (prot.class 1,
degree of protection IP20), the following points should be noted:
• Mains connection
Setting of the mains voltage, checking the mains fuse and the
mains connection must be effected in accordance with the instruc-
tions in section 2.11.
• Opening the 761 SD Compact IC
If the 761 SD Compact IC is connected to the power supply, the in-
strument must not be opened nor must parts be removed from it, oth-
erwise there is a danger of coming into contact with components
which are live. Hence, always disconnect the instrument from all volt-
age sources before you open it and ensure that the mains cable is
disconnected from mains connection
15 !
• Protection against static charges
Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 761 SD Compact IC, you should earth yourself and any
tools you are using by touching an earthed object (e.g. housing of the
instrument or a radiator) to eliminate any static charges which exist.
1.4.2 General precautionary rules
• Handling of solvents
Check all lines of the IC system periodically for possible leaks. Follow
the relevant instructions regarding the handling of flammable and/or
toxic solvents and their disposal.
• Periodic exchange of pump tubing
Pump tubing is consumable material and must be replaced from time
to time (see Section 6.2.9). Please take suitable measures to ensure
that any leakage on pump tubing or connections in unattended con-
tinuous operation does not cause damage (position the instrument at
the bottom and provide a receptacle for catching escaping fluid).

2Installation
761 SD Compact IC / Instructions for Use 8.761.1043
10
2 Installation
2.1 Flow chart
The following flow chart provides an overview of all installation work.
You will find more detailed information in the relevant section.
Setting up sect. 2.2
Installing drain tubes sect. 2.5
Installing eluent flow path sect. 2.6
Installing detektor block sect. 2.4
Installing sample flow path
Installing regenerant flow path sect. 2.7
Replace rear panel
Mains connection sect. 2.11
Installing software sect. 2.12.2
Deaerating/Rinsing sect. 2.13 / 2.14
Connecting PC sect. 2.12
Install guard and column sect. 2.15
sect. 2.10
sect. 2.8
766 IC Sample
Processor Connection to 766 IC Sample Processor
Yes
No
sect. 2.9

2.2 Setting up the instrument
761 SD Compact IC / Instructions for Use 8.761.1043
11
2.2 Setting up the instrument
2.2.1 Packaging
The 761 SD Compact IC is supplied together with the separately
packed accessories in special packagings containing shock-absorbing
foam linings designed to provide excellent protection. The instrument
itself is packed in an evacuated polyethylene bag to prevent the ingress
of dust. Please store all these special packagings as only they assure
transport of the instrument free from damage.
2.2.2 Check
After receipt, immediately check whether the shipment is complete and
has arrived without damage (compare with delivery note and list of
accessories in section 7.2). In the case of transport damage, see
instructions in section 7.4.1 "Warranty".
2.2.3 Location
Position the instrument in the laboratory at a location convenient for op-
eration, free from vibrations and protected against a corrosive atmos-
phere and contamination by chemicals.
To avoid disturbing temperature influences on the insulated column
compartment, the instrument must be protected against direct
sunlight.

2Installation
761 SD Compact IC / Instructions for Use 8.761.1043
12
2.3 Description of the connections
2.3.1 Connection of capillaries/tubing
The connections for eluent, sample and regenerant consist of:
• PEEK capillaries 6.1831.010 (inner diameter = 0.25 mm)
• PTFE microcapillaries 6.1803.030 (inner diameter = 0.5 mm)
• PTFE tubing connections to the MPaks 6.1837.000 (inner di-
ameter = 1.5 mm)
The PEEK capillaries and PTFE microcapillaries can be connected ei-
ther with PEEK pressure screws 50 6.2744.010 (long) or PEEK
pressure screws 51 6.2744.070 (short, for connection to the IC
pump).
The "PTFE tubing connection to the MPak" 23 can be connected to the
PEEK pressure screw 52 4.422.4510 (wide).
The connectors must be fitted to the capillaries as follows in this case:
Capillaries provided with new connectors must feature a flawless, flat
cut edge. In order to ensure this, it is best to use the tubing cutter
6.2621.080.
1 Fit pressure screw
Slide the corresponding pressure screw (50, 51 or 52), as
shown in Figure 4 onto the capillary 53 (23 for MPak connec-
tion).
2 Insert the capillary into the connection
Slide the end of the capillary fully into the corresponding
connector (in order to avoid dead volume).
3 Tighten the pressure screw
Firmly tighten the pressure screw (50, 51 or 52) by hand (do not
use tools).