MiCo BioMed Veri-Q PCR 316 QD-P100 User manual

Veri-Q PCR 316
QD-P100
User Manual
Apr. 2020
Cat No. 9R501

User Instructions
Common name : Gene amplication device
Brand name : Veri-Q PCR 316
Model name : QD-P100
For safe use, please use the device after reading the manual thoroughly.
Please furnish this document at a designated location so it can be used at all times.
MiCo BioMed Co., Ltd.
3rd and 4th Floor , 54 Changeop-ro, Sujeong-gu,
Seongnam-si, Gyeonggi-do, Korea, 13449
TEL: [phone]
FAX: [phone]~2

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Veri-Q PCR 316 QD-P100 User Manual
6. Warranty Policy 58
7. Return Policy 58
8. Classication of Symbols 59
9. Disposal of Electrical & Electronic Equipment 60
10. EMC & Safety Test Results 60
11. Purchase of Reagents and Consumables 61
12. Maintenance Checklist 62
Table of Contents
1. Before you begin 5
1-1. About the manual 5
1-2. General Requirements for Installation 5
1-3. How to Obtain More Information 5
1-4. About Customer Support 5
1-5. Precautions on distribution or duplication of the manual 5
1-6. Laboratory Biosafety 5
2. Precautions when using QD-P100 6
2-1. Purpose of use 6
2-2. Principles of operation 6
2-3. General Precautions 6
3. Product Component and Specication 14
3-1. Product Composition and Characteristic 14
3.2 How to Use 20
3-3 Preparation for Experiment 27
4. How to Install and Use the Operating Program 30
4-1. Execution of Distribution CD and Installation of Operating Program 30
4-2. How to Use the Operating Program 37
4.3. PCR Chip Setting 42
4.4. Operation 48
4-5. Data Analysis 50
5. Troubleshooting 56
5.1. Error Message 56
5.2. Maintenance 57

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Veri-Q PCR 316 QD-P100 User Manual
1. Before You Begin
1.1 About the manual
This manual is prepared for researchers or related staff for using and conducting research using Veri-Q
PCR 316 - QD-P100 and for related staff who installs and maintains the product.
1-2. General Requirements for Installation
If you wish to use this manual, the following is required.
- Pure DNA/RNA suitable for QD-P100 must be used,-When used in connection to a computer, there
should be no problem in using Windows 7 or XP Operating System.-You must have basic knowledge
in handling DNA and RNA samples for PCR.
- You must be familiar with general method of use such as saving, copying, and pasting data on the
hard drive.
※While this equipment can be used as a stand alone without a computer, there should be no
problem in using Windows7 operating system when connected to a computer.
1-3. How to Obtain More Information
To obtain information related to QD-P100, please visit Mico Biomed website(www.micobiomed.com)
or send an e-mail to [email protected].
1-4. About Customer Support
Mico Biomed is using 070-5227-6000 for prompt customer support of the product and to minimize
inconvenience. We are also prepared to listen to our customer’s questions and comments at all times
from the customer’s voice(Q&A) bulletin board at Mico BioMed Co., Ltd. website(www.micobiomed.
com).
1-5. Precautions on distribution or duplication of the manual
The content of this document cannot be altered and duplicated without the permission of the
copyright holder, MiCo BioMed Co., Ltd.
1-6. Laboratory Biosafety
Non-propagative diagnostic laboratory work (e.g. sequencing, NAAT) should be conducted at facilities
and procedures equivalent to BSL-2.

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Veri-Q PCR 316 QD-P100 User Manual
2. Safety Guideline
2-1. Purpose of use
QD-P100 is an instrument for performing real-time polymerase chain reaction (Real-time PCR) that can
rapidly and accurately amplify target nucleic acid for disease diagnosis.
This instrument can be used in the area of in vitro diagnosis, and should be used by a trained
personnel with professional skills. This product is for In Vitro Diagnostic device.
2-2. Principles of operation
This product is the Instrument to amplify nucleic acids (DNAand/orRNA) of specific genes in human
cells by using the principle of Polymerase Chain Reaction (PCR), enabling confirmation of the
amplification and accumulation of genes real time. By using the complementary primer for specific
target genes and the probe labeled with fluorescence dyes, the Instrument measures the fluorescence
generated during PCR amplification, and then measures whether or not the target gene is present in
samples and the amount of the initial template.
The value of fluorescence generated to amplify the genes with PCR is recorded in Photodiode.The
fluorescent chromosomes mainly used are FAM, HEX, TEX, and CY5, and it is possible to measure them
entirely or selectively by using the power LED light sources photodiode, and light filters.
2-3. General Precautions
QD-P100 System must be used by a trained personnel with specialized skills related to genetic analysis.
Please read this manual before operating the device. If the usage method and precautions in the
manual is not followed, it may threaten the safety of the personnel using the device. Please read and
follow the warning indicated in the sign.
WARNING – It may cause the risk of physical injury or death or damage to the device
if the guideline or procedure is not followed.
Waste – Do not dispose of the product with general household waste. Local waste
collection regulation for electronic and electric products must be followed.
2-3-1. Warning and Prevention
WARNING
QD-P100 System is an electronic and mechanical device. Since there is a risk of electric shock or
physical injury, you must operate the device according to the user manual. The manufacturer is not
held responsible for problems arising from not observing the safety precautions. Follow the safety
precautions attached to the device.
1. Please comply with general safety requirements applied to the electronic device.
2. Do not access the internal part of the equipment while it is turned on.
3. Do not move the device while the PCR is under operation.
4. When cleaning the device, perform cleaning after turning off the power and unplugging the
power cord.
5. Do not use the device near water.
6. Prep reagent, solvent, etc. can affect the PCR results.
7. Purified or concentrated nucleic acid (DNA/RNA) can affect the PCR results.
8. Do not touch the switch, power cable or device with wet hands.
9. Do not randomly dismantle or remodel the device. It may cause fire, electric shock, and
breakdown, and you will not be able to receive after service.
10. Only a designated engineer can provide repair and services.
11. Do not overload the power outlet. It may cause fire or electric shock.
12. Please install the device on a clean and level surface. Corrosive gas or high density magnetic
location can damage the device. Do not install on an unstable or sloped location. If the device
is wet, immediately unplug the power cable.
13. Do not use the device for purposes other than gene amplification or real-time quantitative
analysis. It may cause malfunction of the product or injury.
14. Do not use components other than the ones provided by the manufacturer. It may cause the
malfunction or breakdown of the product.
15. In case of any one of the following conditions, unplug the power cable and make an inquiry to
the manufacturer.
▶If power cord or plug is damaged
▶If the device is exposed to water or liquid
16. Do not place items on the device or sit on it.
17. This equipment has been designed according to CISPR11, Class A. In a domestic environment,
it may receive interference, and in such a case, you may be required to take measures for
alleviating the interference.
18. Evaluation on the electromagnetic environment should be conducted prior to the operation
of the device.
19. Do not use this device near sources of powerful electromagnetic radiation (e.g. Unshield
intentional RF sources). These may interfere with normal operation.

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Veri-Q PCR 316 QD-P100 User Manual
● In case of an emergency, immediately unplug the power cable.
Or turn o the power switch.
WARNING–Chemical Safety
Refer to MSDS before handling chemical substances, and comply with all related precautions.
The waste generated while using the device may cause injury, disease or death. Refer to the MSDS
and local regulations for handling, storing, and disposal of wastes.
WARNING – Chemical Waste Disposal
Please wear appropriate protective gear such as goggles, garment, gloves, etc. when disposing
reagents and chips. You must wear safety gear when handling toxic substance and substance which
cause infection. Store, transport and dipose the wastes according to all local, regional or national
regulations.
Caution –LED Safety
You may be exposed to LED if you do not comply with safety precautions.
2-3-2. Shipping and Transport
QD-P100 System is packaged in a hard case, and is shipped in a box.
The hard case includes QD-P100 System and its components. The product componentss, specification,
PCR installation, and usage method are explained in detail later on. Even when warranty period is
valid, abnormality that has occurred due to inappropriate packaging and transport is excluded from
the warranty.
1. Condition during transport and storage
Allowable temperature Room temperature
Relative humidity 20% to 90% (without condensation)
2. Shipping method
Use the hard case included as a component when moving or transporting the device. The device
must be transported in a hard case.
1) Open the case. 2) Place the device in the hard case.
3) Close the hard case. 4) Lock the case.

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Veri-Q PCR 316 QD-P100 User Manual
5) Transport while carrying it with a hand.
Please proceed in reverse order when unpacking.
2-3-3. Operating Environment and Installation
If precautions are not followed when installing the device, it may cause risk. Please follow the
guideline.
1. This device is a precise optical device. Please install the device away from direct sunlight and
strong light.
2. Check the power condition and use the device. The product can use both 220V/110V. For
stable use of the product, use of automatic voltage regulator (AVR) or UPS (Uninterrupted
Power Supply with AVR function is recommended.
3. Use grounded power supply. If the device is not grounded, you can incur physical injury by
lightning, and cannot use the product stably.
4. Do not use the power plug when it is loose. The plug becomes damaged due to overheating
and may cause fire or electric shock.
5. Wipe dust off the power plug, and plug it firmly so it does not shake. If the connection is
unstable, it becomes the cause of fire.
6. Do not use multiple devices on a power outlet at the same time. Abnormally generated
heating of the power outlet due to overload becomes the cause of fire.
7. When installing the product, install the system body at least over 10 cm away from the wall
and over 20 cm away from other items. If the product is installed close to the wall, it becomes
the cause of product breakdown.
8. Do not place items that can interfere with the operation around the product. This may interfere
with the operation of the product, and abnormality may occur when operating the door.
9. Install the device away from a place with a lot of dust. It becomes the cause of malfunction or
breakdown of the product.
10. Install away from heating devices such as electric heating appliance. It becomes the cause of
fire.
11. Install the device away from a place where water splashes or a place with much moisture. It
becomes the cause of electric shock, fire, and breakdown.
12. Do not install the device in a place where flammable or corrosive gas is generated. In case of
gas leakage, do not operate the power plug of the device but open the window for ventilation.
Sparks may fly and cause an explosion and fire.
13. Do not randomly dismantle or remodel the device. It may cause fire, electric shock, and
breakdown, and you will not be able to receive after service.
CLASS 1 LED PRODUCT
< QD-P100 System is an LED Grade Class 1 product. >
CAUTION - CLASS 2M LED
RADIATION WHEN OPEN
DO NOT STARE INTO THE BEAM OR
VIEW DIRECTLY WITH OPTICAL
INSTRUMENTS
< Class 2M LED module is used inside QD-P100 System. >
1. Conditions
- Operating temperature: 15 ~ 32°C
- Operating humidity: 20 ~ 80 %
- Max. 80% without condensation (under 32°C conditions)
- Min. 20% (under 15 ~ 32°C conditions)
2. LED Module
- Please refer to page 15 for product specification.
- Frequency: LED is turned on about every 4 seconds per cycle. It does not light up if the device
is not operating.

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Veri-Q PCR 316 QD-P100 User Manual
CAUTION
The computer should have a communication port for USB data transfer. Do not install a memory stick
when the device is operating. A problem may occur in the communication between the device and
the computer.
2-3-4. Consumable
A disposable consumable is required for the operation of the device. Labchip is produced only for the
QD-P100. Do not use the chip more than 2 times. It may bring about unreliable results.
Disposable Description
QD-P100 Labchip
Pour a set amount of reagent per channel in
the Labchip and close the chip case.
2-3-5. Warning and Caution for Use and Management
The QD-P100 System does not require regular cleaning.
1. Warning and Caution for Use and Management
- Do not use the device for purposes other than gene amplification or real-0time quantitative
analysis. It may cause malfunction of the product or injury.
- Manage the LabChip part so that it is not stained with foreign substances. It may cause
product breakdown or injury, and accurate experiment data cannot be obtained.
- Do not use the device while placing paper or bottom board made out of synthetic resin
underneath the device. It may cause fire.
- Do not cover the product with paper, vinyl, etc. when the device is in operation. It becomes
the cause of fire and breakdown of the device.
- When using the device in connection to a computer, set “Turn off monitor”, “Turn off hard disk”,
“System standby mode”, and“System hibernate mode”in power option as“Not used.”
-
If the QD-P100 is operated in connection to a computer, since it communicates a large amount
of data in real-time, an error may occur during operation if the monitor or hard disk is turned off.
- If the device malfunctions, an error indication lamp lights up and an error code is displayed on
the LCD screen. If an error occurs, do not operate the device by force and ask the head office
for assistance.
- If the product is randomly dismantled, the user can be exposed to the LED, and if the user’s
eyes are exposed to it, an injury may be incurred.
- Since high temperature conditions are maintained inside this product, the user can be burned
when exposed. Do not touch the thermal block. You must be careful since the thermal block is
hot when the device is operating.
- Do not recharge the coin battery. If the battery is replaced in a wrong manner, there is a risk of
an explosion.
WARNING
The power cable must be unplugged when cleaning the device.
WARNING
Do not pour liquid into the chip door.
Transport, Storage and Preservation Method
Under operation In storage In transport
Temperature (℃)15 ~ 32 Room temperature (RT) Room temperature (RT)
Relative humidity (%) 20 ~ 80 20 ~ 90 20 ~ 90
Pressure (kPa) 70 ~ 100 70 ~ 100 70 ~ 100
- Store the device at a location with no moisture.
- Do not store at a location adversely affecting the device with pressure, temperature, humidity,
ventilation, dust, and air containing salt, etc.
- Be careful of safety conditions such as slope, vibration, shock, etc. (including when driving a car)
- Regular inspection must be performed on the device and its components.
- When re-using the device that has not been used for a while, make sure the device is clean and
check whether it works safely before use.
2-3-6. Limitation
The QD-P100 is optimized to use with Veri-Q PCR 316 reagent kit made by Mico BioMed and it couldn’t
guaranteed performance excepting the system.

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Veri-Q PCR 316 QD-P100 User Manual
3. Product Component and Specication
3-1. Product Composition and Characteristic
The warranty period of the product is 1 year from the date of purchase. However, in case of a
breakdown due to the user’s negligence, free warranty is not available. If a breakdown occurs after the
warranty period, a fee will be charged for the repair. Before installing the product, check whether all
items in the following product composition is included in the product package.
3-1-1. Product Composition
Component Quantity Check (O,X)
QD-P100 Instrument 1
Real-time PCR QD-P100
- software
- Guides
- Drivers
1
AC Adapter
- Model designation : SNP-A127-M
- Manufacturer : SKYNET ELECTRONIC CO LTD,
- Input : 100-250V, 3-1,5 A, 50 / 60 Hz
- Output : 12 V dc, 9 A
1
Main power cable (EU) 1
USB cable A to B 1
Labchip case 1
Labchip 1
Rubber 1
Optional Components
Chip cradle 1
SW CD 1
Common name Gene amplification device
Brand name Veri-Q PCR 316
Model name QD-P100
Software QD-P100 RTPCR_2.00 Ver
Temperature range 40 ~ 120 ℃
Temperature accuracy ±0.3℃at 90℃setting condition
Temperature uniformity ±0.3℃within 30 seconds at 90°C setting
Input rating 12 V dc
Input current 9A
Detection Photo diode
Ports USB
Weight 4.7Kg
Dimension 274x170x206mm
Fluorescence Wavelength
(Excitation)
FAM – 483±18nm
HEX – 534±12.5nm
Cy5 – 628±19nm
Texas Red – 586±13nm
Fluorescence Wavelength
(Emission)
FAM – 535±24nm
HEX – 572±16.5nm
Cy5 – 692±23.5nm
Texas Red – 623±15nm
Fluorescence type FAM, HEX, Cy5, Texas Red,
Battery Coin battery (CR2032)

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Veri-Q PCR 316 QD-P100 User Manual
3-1-3. Device Structure and Name
1. Front View
2. Top View
3. Rear View
4. Side View
3-1-4. How to Install Product
1. Device Connection
1) Open the hard case.
2) Check whether nothing is wrong
with the components and the
device.
3) Connect the adapter to the power
port.
4) Connect the AC inlet of the AC
adapter to the main power cable.
5) Connect to the power outlet.

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Veri-Q PCR 316 QD-P100 User Manual
6) Install QDInstallManager.exe
2. Computer Connection
In the back of QD-P100 system, there is a power connection port, and a data transmission cable
connection (USB port) for sending and receiving data to and from the computer.
Follow the following procedure to install the QD-P100 system and connect it to the computer.
7) Connect the cable to the USB port at
the back of QD-P100 system.
8) Connect the opposite side of the
USB cable to the computer.
3. Precautions upon Installation
Please install the product according to the manual. If the location of the product installation is
wrong, the device can be damaged.
- Install the device away from direct sunlight.
- Unplug the cable and turn off the power before installation. It may damage the computer or
the device.
- Check if the USB is properly connected. If the connection is unstable, an error may occur upon
data transmission.
- Please be careful to prevent damage of the device due to static.
- Installation Location
The backside should be installed more than 10cm away from the wall.
The side of the device should be installed more than 20cm away from the wall or other items.
4. Product Installation
The QD-P100 System is a Stand alone device that can be operated without a computer.
The product’s operating program should be installed on the computer in order to operate
QD-P100 system in connection to a computer. Please refer to this chapter if you are installing for
the first time or if you need to inevitably reinstall the program on the computer installed upon
purchasing the product. For re-installation, you must follow the order by referring to this chapter.
You must first prepare a desktop or laptop in order to use the QD-P100 system in connection
to the computer. The recommended requirement for the computer suitable for using the QD-
P100 system is as follows. For other inquiries, please contact the customer support center of MiCo
BioMed. If the computer does not satisfy the recommended requirement, the QD-P100 system
will not work properly or a problem may arise when the device is operated.
Recommended Requirement of Computer
1) Intel Dual Core E2160 (1.8GHz) or higher
2) Window 7 for Korean or English Version
3) 2.0GB RAM or higher
4) Screen resolution: 1920 * 1280 or higher
5) USB 2.0 high speed port
6) 20GB hard disk drive minimum
7) Microsoft Excel, Word (Option)
For matters regarding the computer and installation of OS not directly related to QD-P100, please
make an inquiry to the computer dealer or computer after service support center.

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Veri-Q PCR 316 QD-P100 User Manual
3.2 How to Use
3-2-1. Device Operation and Experiment Preparation
If the device and the computer are installed and connected according to the product installation
method, check the normal operation of the device and start the experiment.
3-2-2. Power Supply and Self Test Method
1. Connect the adapter cable to the power connector at the back side of the QD-P100 body.
(**Caution: Based on the direction of viewing the power connector, the direction of the arrow of
the adapter cable should face upwards. If the adapter cable is inserted in the opposite direction,
even if it does not fit, it can be forced in with strong force, and in this case, the electronic circuit
can be critically damaged.)
2. If you connect the adapter cable and turn on the device, it boots up and initializes. The LCD
screen displays the company logo until initialization is completed, and a blue light will light up
on Run LED (status LED). If the initialization is finished, it switches over to the main menu screen,
and a green light turns on. The green light coming on means the device is working properly.
3-2-3. Description of Program Screen
■The main screen is displayed several minutes after the device is connected to the power.

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Veri-Q PCR 316 QD-P100 User Manual
■Select the light source of the sample to be measured.
■Select a number of protocol steps.
■Set the number of cycles of the sample to be measured.
■Set the temperature and time of the sample to be measured.

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Veri-Q PCR 316 QD-P100 User Manual
■The door of the device can be opened and closed by pressing Chip In/Out button.
■The device can be operated by pressing RUN START button.
■Progress can be checked in the View tab while PCR is in progress.

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Veri-Q PCR 316 QD-P100 User Manual
■When PCR is finished, a pop-up window informing that the PCR is finished pops up.
■ The completed PCR data is saved inside, and the saved data can be checked immediately
by printing it through the printer built into the device.
3-3 Preparation for Experiment
For sample preparation, see the instructions for use of Veri-Q PCR 316 reagent kit made by Mico
BioMed
3-3-1. Sample Insertion
Insert the prepared sample into the chip.
3-3-2. Preparation of LabChip Case and Rubber Cover
Prepare the chip case and rubber cover as shown in the photo below.

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Veri-Q PCR 316 QD-P100 User Manual
3-3-3. LabChip Case Mounting
As shown in the photo below, the left part is the bottom board and the right part is the part that will
cover the left part. Place the rubber cover on the right part to fit the shape, and place the chip on the
left part.
Cover the right part with the left part and pull the fixing clip to either side as shown in the photo
below.
3-3-4. LabChip Insertion
Chip door will automatically open once the device is turned on. When inserting the chip case, the
circled part in the following photo must head toward the device.
Gently push and insert it until a “click” sound is heard. (Caution – While the chip case is being
completely inserted, do not forcibly push the chip case.)
3-3-5. LabChip Removal
Once the experiment is finished, the chip door will automatically open. Press the chip in the direction
of insertion until a “click” sound is heard, and then, take out the chip case and remove the rubber cover
and the chip.
※Do not re-use the Labchip since it is disposable.

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Veri-Q PCR 316 QD-P100 User Manual
4. How to Install and Use the Operating Program
4-1. Execution of Distribution CD and Installation of Operating Program
4-1-1. Execution of Distribution CD
<Figure 1> QD-P100 Distribution Program Folder and Execution File
1. If QD-P100 Install Manager distribution CD is inserted into the computer, you can check the
folders and files as shown in <Figure 1>.
2. Run the execution file,“QD-P100 Install manager.exe”file.
<Figure 2> QD-P100 Install Manager Program Execution Screen
4-1-2. CP210x Driver Install(32bit, 64bit)
<Figure 3> CP210x Driver Install Button
1. Select “CP210x 32Bit Driver Install”, or “CP210x 64Bit Driver Install”button as shown in <Figure 3>.
<Figure 4> CP210x 64Bit Driver Install Execution Screen-1
2. Select “Next”button as shown in <Figure 4>.

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Veri-Q PCR 316 QD-P100 User Manual
<Figure 5> CP210x 64Bit Driver Install Execution Screen-2
3. Select “I accept this agreement” and then “Next” button as shown in <Figure 5>.
<Figure 6> CP210x 64Bit Driver Install Execution Screen-3
4. The driver is installed as shown in <Figure 6>.
<Figure 7> CP210x 64Bit Driver Install Execution Screen-4
5. Select “Finish”button as shown in <Figure 7>.
4-1-3. QD-P100 Software Install
<Figure 8> QD-P100 Software Install Execution Screen-1
1. Select “QD-P100 Software Install” button as shown in <Figure 8>.

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Veri-Q PCR 316 QD-P100 User Manual
<Figure 9> QD-P100 Software Install Execution Screen-2
2. Select “Next” button as shown in <Figure 9>.
<Figure 10> QD-P100 Software Install Execution Screen-3
3. Select “Install”button as shown in <Figure 10>.
<Figure 11> QD-P100 Software Install Execution Screen-4
4. Select “Finish”button and finish installation as shown in <Figure 11>.
4-1-4. Manual View
<Figure 12> Manual View Execution
1. If “Manual View” button is selected as shown in <Figure 12>, you can check the Manual file in PDF format.

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Veri-Q PCR 316 QD-P100 User Manual
4-1-5. DaViewIndy(PDF Viewer)
<Figure 13> DaViewIndy Installation
1. If “DaviewIndy” button is selected as shown in <Figure 13>, you can install PDF Viewer program.
4-2. How to Use the Operating Program
4-2-1. Working Wizard
In the working wizard, you can view the list of settings previously experimented and the list of result
files.
<Figure 1> QD-P100 rtPCR Working Wizard
1. Connection Status
Generally, you should connect the communication cable between RTPCR device and PC, and run the
program while the power is turned “On.”
If properly connected:
If properly connected, you can view only the screen of <Figure 1>.
If not properly connected:
If improperly connected, you can view a pop-up window as shown in <Figure 2>.
<Figure 2> Pop-up for improper connection