MiCo BioMed Veri-Q PCR 316 QD-P100 User manual

Veri-Q PCR 316
QD-P100
User Manual
Apr. 2020
Cat No. 9R501

User Instructions
Common name : Gene amplication device
Brand name : Veri-Q PCR 316
Model name : QD-P100
For safe use, please use the device after reading the manual thoroughly.
Please furnish this document at a designated location so it can be used at all times.
MiCo BioMed Co., Ltd.
3rd and 4th Floor , 54 Changeop-ro, Sujeong-gu,
Seongnam-si, Gyeonggi-do, Korea, 13449
TEL: [phone]
FAX: [phone]~2

2 3
Veri-Q PCR 316 QD-P100 User Manual
6. Warranty Policy 58
7. Return Policy 58
8. Classication of Symbols 59
9. Disposal of Electrical & Electronic Equipment 60
10. EMC & Safety Test Results 60
11. Purchase of Reagents and Consumables 61
12. Maintenance Checklist 62
Table of Contents
1. Before you begin 5
1-1. About the manual 5
1-2. General Requirements for Installation 5
1-3. How to Obtain More Information 5
1-4. About Customer Support 5
1-5. Precautions on distribution or duplication of the manual 5
1-6. Laboratory Biosafety 5
2. Precautions when using QD-P100 6
2-1. Purpose of use 6
2-2. Principles of operation 6
2-3. General Precautions 6
3. Product Component and Specication 14
3-1. Product Composition and Characteristic 14
3.2 How to Use 20
3-3 Preparation for Experiment 27
4. How to Install and Use the Operating Program 30
4-1. Execution of Distribution CD and Installation of Operating Program 30
4-2. How to Use the Operating Program 37
4.3. PCR Chip Setting 42
4.4. Operation 48
4-5. Data Analysis 50
5. Troubleshooting 56
5.1. Error Message 56
5.2. Maintenance 57

4 5
Veri-Q PCR 316 QD-P100 User Manual
1. Before You Begin
1.1 About the manual
This manual is prepared for researchers or related staff for using and conducting research using Veri-Q
PCR 316 - QD-P100 and for related staff who installs and maintains the product.
1-2. General Requirements for Installation
If you wish to use this manual, the following is required.
- Pure DNA/RNA suitable for QD-P100 must be used,-When used in connection to a computer, there
should be no problem in using Windows 7 or XP Operating System.-You must have basic knowledge
in handling DNA and RNA samples for PCR.
- You must be familiar with general method of use such as saving, copying, and pasting data on the
hard drive.
※While this equipment can be used as a stand alone without a computer, there should be no
problem in using Windows7 operating system when connected to a computer.
1-3. How to Obtain More Information
To obtain information related to QD-P100, please visit Mico Biomed website(www.micobiomed.com)
or send an e-mail to [email protected].
1-4. About Customer Support
Mico Biomed is using 070-5227-6000 for prompt customer support of the product and to minimize
inconvenience. We are also prepared to listen to our customer’s questions and comments at all times
from the customer’s voice(Q&A) bulletin board at Mico BioMed Co., Ltd. website(www.micobiomed.
com).
1-5. Precautions on distribution or duplication of the manual
The content of this document cannot be altered and duplicated without the permission of the
copyright holder, MiCo BioMed Co., Ltd.
1-6. Laboratory Biosafety
Non-propagative diagnostic laboratory work (e.g. sequencing, NAAT) should be conducted at facilities
and procedures equivalent to BSL-2.

6 7
Veri-Q PCR 316 QD-P100 User Manual
2. Safety Guideline
2-1. Purpose of use
QD-P100 is an instrument for performing real-time polymerase chain reaction (Real-time PCR) that can
rapidly and accurately amplify target nucleic acid for disease diagnosis.
This instrument can be used in the area of in vitro diagnosis, and should be used by a trained
personnel with professional skills. This product is for In Vitro Diagnostic device.
2-2. Principles of operation
This product is the Instrument to amplify nucleic acids (DNAand/orRNA) of specific genes in human
cells by using the principle of Polymerase Chain Reaction (PCR), enabling confirmation of the
amplification and accumulation of genes real time. By using the complementary primer for specific
target genes and the probe labeled with fluorescence dyes, the Instrument measures the fluorescence
generated during PCR amplification, and then measures whether or not the target gene is present in
samples and the amount of the initial template.
The value of fluorescence generated to amplify the genes with PCR is recorded in Photodiode.The
fluorescent chromosomes mainly used are FAM, HEX, TEX, and CY5, and it is possible to measure them
entirely or selectively by using the power LED light sources photodiode, and light filters.
2-3. General Precautions
QD-P100 System must be used by a trained personnel with specialized skills related to genetic analysis.
Please read this manual before operating the device. If the usage method and precautions in the
manual is not followed, it may threaten the safety of the personnel using the device. Please read and
follow the warning indicated in the sign.
WARNING – It may cause the risk of physical injury or death or damage to the device
if the guideline or procedure is not followed.
Waste – Do not dispose of the product with general household waste. Local waste
collection regulation for electronic and electric products must be followed.
2-3-1. Warning and Prevention
WARNING
QD-P100 System is an electronic and mechanical device. Since there is a risk of electric shock or
physical injury, you must operate the device according to the user manual. The manufacturer is not
held responsible for problems arising from not observing the safety precautions. Follow the safety
precautions attached to the device.
1. Please comply with general safety requirements applied to the electronic device.
2. Do not access the internal part of the equipment while it is turned on.
3. Do not move the device while the PCR is under operation.
4. When cleaning the device, perform cleaning after turning off the power and unplugging the
power cord.
5. Do not use the device near water.
6. Prep reagent, solvent, etc. can affect the PCR results.
7. Purified or concentrated nucleic acid (DNA/RNA) can affect the PCR results.
8. Do not touch the switch, power cable or device with wet hands.
9. Do not randomly dismantle or remodel the device. It may cause fire, electric shock, and
breakdown, and you will not be able to receive after service.
10. Only a designated engineer can provide repair and services.
11. Do not overload the power outlet. It may cause fire or electric shock.
12. Please install the device on a clean and level surface. Corrosive gas or high density magnetic
location can damage the device. Do not install on an unstable or sloped location. If the device
is wet, immediately unplug the power cable.
13. Do not use the device for purposes other than gene amplification or real-time quantitative
analysis. It may cause malfunction of the product or injury.
14. Do not use components other than the ones provided by the manufacturer. It may cause the
malfunction or breakdown of the product.
15. In case of any one of the following conditions, unplug the power cable and make an inquiry to
the manufacturer.
▶If power cord or plug is damaged
▶If the device is exposed to water or liquid
16. Do not place items on the device or sit on it.
17. This equipment has been designed according to CISPR11, Class A. In a domestic environment,
it may receive interference, and in such a case, you may be required to take measures for
alleviating the interference.
18. Evaluation on the electromagnetic environment should be conducted prior to the operation
of the device.
19. Do not use this device near sources of powerful electromagnetic radiation (e.g. Unshield
intentional RF sources). These may interfere with normal operation.