Misonix SONICATOR XL2020 User manual

SONICATOR®
ULTRASONIC LIQUID PROCESSOR
OPERATION MANUAL
M I S O N I X
MISONIX INCORPORATED
MODELS
XL2020
XL2015
XL2010
MISONIX INCORPORATED
1938 New Highway, Farmingdale, NY 11735 U.S.A.
Telephone: [phone] Fax: [phone]
Toll Free: 1-800-645-9846
www.misonix.com

TABLE OF CONTENTS
1. Safety - Warnings and Precautions 1-1
2. Getting Started 2-1
Inspection 2-1
Power Requirements 2-1
Placement of Equipment 2-1
System Components Diagram 2-2
3. Specifications for XL2020, XL2015 & XL2010 3-1
4. Description of Major Components 4-1
Generator (Power Supply) 4-1
Process Control Features of Model XL2020 4-1
Process Control Features of Model XL2015 4-1
Convertor (Transducer) 4-2
Horn (Probe) 4-2
Replacement Flat Tips 4-2
5. Function of Controls and Indicators 5-1
XL2020 Functions 5-1
XL2020 Controls and Indicators Diagram 5-2
XL2010 & XL2015 Functions 5-3
XL2010 & XL2015 Controls and Indicators Diagram 5-4
6. Preparation for Use 6-1
Convertor Mounting Diagram 6-2
Removal and Attachment of Horns and Tips 6-3
Removal and Attachment of Horns and Tips Diagram 6-4
7. Tuning Instructions 7-1
XL2020 Tuning Instructions (Probes & Horns) 7-1
XL2010 & XL2015 Tuning Instructions (Probes & Horns) 7-2
MicrotipTM and Cup Horn Instructions 7-3
8. Programming the Microprocessor of XL2020 8-1
Review of Keys 8-1
Keypad Diagram 8-1
Continuous Mode 8-2
Timer Mode Only 8-3
PulsarTM Mode Only 8-4
Timer and PulsarTM Mode 8-5
Storing Programs into Memory 8-6
9. Accessories 9-1
Cup Horn 9-1
Installation 9-1, 9-2
Operation 9-2
Output Setting Parameters 9-2
Maintenance 9-3
Continuous Flow Cell 9-3, 9-4
MicrotipTM 9-5

Stepped MicrotipTM 9-5
Extender Tip 9-6
Sapphire Tipped Horn 9-6, 9-7
Sonabox 9-7
Dual Horn 9-7
Tray Horn 9-7
Sonabox Diagram 9-8
10. Troubleshooting 10-1
Overload Indicator Instructions 10-1
Troubleshooting Guide 10-1
11. Maintenance 11-1
General Maintenance 11-1
Cleaning Instructions 11-1
Tip Care 11-2
12. Applications 12-1
Principle of Ultrasonics 12-1
Processing Tissue 12-1
Processing Difficult Cells 12-2
13. Techniques 13-1
Probe Depth 13-1
Foaming and Aerosoling 13-1
Viscosity Limitations 13-1
Keeping Samples Cool 13-1
Free Radical Formation 13-1
14. Warranty 14-1
15. Return of Equipment 15-1
16. Literature Request 16-1
Bibliographies 16-1
Application Notes 16-1
Published Papers 16-2
Technical Notes 16-2
Technical Bulletins 16-2

Operation Manual ____________________Safety – Warnings and Precautions
_________________________________________________________________________________________________
1-1
1. SAFETY - WARNINGS AND PRECAUTIONS
Read ALL Instructions Before Installing or Using Equipment
Your new Ultrasonic Liquid Processor has been designed, built and tested to assure
maximum operator safety. However, no design can completely protect against improper
usage which may lead to bodily injury and/or property damage. For total safety and
equipment protection, read the instruction manual carefully before attempting to operate
this equipment. Observe the following WARNINGS:
•High voltage is present in the generator (power supply), convertor and high frequency cable.
There are no user-serviceable parts inside any of these devices. Do NOT attempt to
remove the generator cover or convertor case.
•Do NOT touch any open cable connections on the unit while the power is turned ON.
•Do NOT operate generator with convertor disconnected from high voltage cable. High
voltage is present in the cable and may pose a shock hazard.
•The generator must be properly grounded with a 3-prong plug. Test electrical outlet for
proper grounding before plugging in unit.
•Install the SONICATOR®in an area free from excessive dust, dirt, explosive or corrosive
fumes and protected from extremes in temperature and humidity.
•NEVER immerse the convertor in liquids of any kind, or let condensed moisture or liquid drip
into the convertor.
•NEVER grasp an activated horn or touch the tip of a vibrating probe. It can cause severe
burns and tissue damage.
•NEVER hold or clamp the convertor by the front driver (black portion) or by the horn itself.
This can cause permanent damage to the system. Support the convertor by clamping only
around the broad (chrome) section.
•Do NOT allow the tip of a vibrating horn or probe touch the counter top or any other hard
surface. It could damage the probe or overload the generator.
•Avoid touching the bottom or sides of a glass or plastic container with a vibrating probe. It
could crack or shatter the glass or melt the plastic.
•Turn OFF the power switch, unplug the generator and disconnect the power cord from the
back of the generator before attempting to replace the fuse.

Operation Manual __________ Getting Started
_________________________________________________________________________________________________
2-1
2. GETTING STARTED
INSPECTION
Your new SONICATOR®Ultrasonic Liquid Processor was thoroughly inspected, tested and
carefully packed before leaving the factory. Prior to unpacking, carefully inspect the
shipping carton for any evidence of damage. Claims for loss or damage sustained in
transit, must be made to the shipping company.
Unpack the unit from its shipping carton and check the contents against the packing list.
Before disposing of the packing material, check it carefully for small items. Report any
missing components to Misonix immediately.
Visually inspect all external controls, indicators and surfaces to detect any damage in
transit. If damage has occurred, contact your carrier within 48 hours of delivery date. DO
NOT OPERATE DAMAGED EQUIPMENT. Retain all packing materials for possible future
shipments.
POWER REQUIREMENTS
For power requirements, check the label on the back of the unit.
1. For 110V operation, the generator requires a single phase, three wire, 110V, 50/60 Hz
line supply, unless otherwise specified.
2. For 220V operation, the generator requires a single phase, three wire, 220V, 50/60 Hz
line supply, unless otherwise specified.
WARNING!!
The electrical line cord is equipped with a 3-prong grounding plug. Do not, under any
circumstances, remove the grounding prong. The plug must be plugged directly into
a mating 3-prong grounding type outlet.
PLACEMENT OF EQUIPMENT
Place the Generator in an area that is free from excessive dust, dirt and
explosive or corrosive fumes. A fan maintains safe operating temperature in the
generator by circulating air over the electronic components. Therefore, the generator must
be placed so that the air intake on the rear panel is not blocked. Clean and inspect the air
intake grill periodically, to insure it is free from dust and debris.

Operation Manual _________________________Specifications for XL2020, XL2015 & XL2010
______________________________________________________________________________________________
3-1
3. SPECIFICATIONS FOR XL2020, XL2015 & XL2010
Generator 110 Vac 220 Vac
Input Voltage 100-130 Vac @ 50/60 Hz 200-260 Vac @ 50/60 Hz
Full Load Current 10 Amps 7.5 Amps
Fuse Rating 10 Amps (GDB10)* 8 Amps (GDB8)*
Weight 14 lbs. (6.3Kg) 16.5 lbs. (7.4Kg)
Dimensions 7.5"x18.5"x11.6" (WxLxH)
19cm x 47cm x 29.5cm
7.5"x18.5"x11.6" (WxLxH)
19cm x 47cm x 29.5cm
Output Voltage 1500 V rms (max.) 1500 V rms (max.)
Output Frequency 20 KHz (nom.) 20 KHz (nom.)
Convertor
Weight 2 lbs. (0.9 Kg) 2 lbs. (0.9 Kg)
Dimensions 8" L x 2.5" Dia.
(20.3cm x 6.4cm)
8" L x 2.5" Dia.
(20.3cm x 6.4cm)
Materials Aluminum Aluminum
Standard Horn
Weight 0.5 lbs. (0.45Kg) 0.5 lbs. (0.45Kg)
Dimensions 5" L x1.5" Dia.
(12.7cm x 3.8cm)
5" L x1.5" Dia.
(12.7cm x 3.8cm)
Materials Titanium Alloy Titanium Alloy
Environmental
Pollution Degree Two (2) Two (2)
Installation Category II II
Temperature Limits 50 F to 110 F
(10 C to 43 C)
50 F to 110 F
(10 C to 43 C)
Barometric Pressure Unlimited** Unlimited**
* Only use IEC approved fuses, model GDB10 for 110V and GDB8 for 220V. Do NOT use “slow
blow” fuses or fuses rated above the amperage noted.
** In a Vacuum area, additional cooling provisions may be needed.
ACCESSORIES
Use only accessories and probes listed in the Misonix catalog as suitable for use with this
equipment. Do NOT attempt to fabricate ultrasonic tooling or accessories unless approval has
been obtained from Misonix in advance.

Operation Manual __________________________________Description of Major Components
______________________________________________________________________________________________
4-1
4. DESCRIPTION OF MAJOR COMPONENTS
GENERATOR (POWER SUPPLY)
Includes all operating controls and indicators, detachable three-wire grounded line cord,
fuse, external power control jack and high frequency cable connector.
PROCESS CONTROL FEATURES OF MODEL XL2020
•Microprocessor Controller - allows precise control of output power, elapsed processing
time, and cyclical rates of power pulsing. It encompasses the functions of the Digital
Integrating Timer and the Multi-Range PULSAR™ Cycle Timer.
•Digital Integrating Timer - permits precise timing of ultrasonic energy applied in one
second increments from one second to ten hours. The total desired processing time is set
on a pressure-sensitive keypad. Both the processing time selected and actual total elapsed
processing time is indicated by an LCD readout. A start/stop control initiates or reinitiates
timed sonication. A pause control allows manual pulsing or delay. The total preset time of
active sonication is unaffected by pauses or pulsing, whether by hand or with the Multi-
Range PULSAR™ Cycle Timer.
•Multi-Range PULSAR™ Cycle Timer - permits the application of ultrasonic energy to the
sample on a pulsed basis. Pulse on and off times are adjustable in 0.1 second increments
from 0.1 seconds to 1 hour each. This intermittent operation permits high intensity
sonication while avoiding heat build-up in the processed sample. It also allows particulate
material being processed to settle back under the tip.
•OVERLOAD INDICATOR - under certain extraordinary conditions, the amount of power
supplied to the convertor/horn assembly might endanger the circuitry of the generator to the
point of failure. In order to prevent unnecessary downtime, an overload protection feature
has been built into the generator. When the output power exceeds design specifications,
the overload light will illuminate and the output will be disabled. The following prompt will
appear: "OVERLOAD REFER TO MANUAL". (See 10-1 for instructions)
PROCESS CONTROL FEATURES OF MODEL XL2015
•PULSAR™ Cycle Timer - permits the application of ultrasonic energy to the sample on a
pulsed basis. Energy is automatically set for one pulse per second. Pulse width adjusts
smoothly from 0.05 of a second (5% duty) to 0.95 of a second (95% duty) every second.
This intermittent operation permits high intensity sonication while avoiding heat build-up in
the processed solution. It also allows material being processed to settle back under the tip.
•OVERLOAD INDICATOR - under certain extraordinary conditions, the amount of power
supplied to the convertor/horn assembly might endanger the circuitry of the generator to the
point of failure. In order to prevent unnecessary downtime, an overload protection feature
has been built into the generator. When the output power exceeds design specifications,
the overload light will illuminate and the output will be disabled. (If this should occur, follow
instructions in 10-1)

Operation Manual _____________________Description of Major Components
_________________________________________________________________________________________________
4-2
CONVERTOR (TRANSDUCER)
Includes the electroplated housing, and black front driver (first stage of acoustic
amplification) with a ½ - 20 threaded hole for horn attachment. A separate high-frequency
cable is also furnished.
HORN (PROBE)
A 5" L x 1½" W resonant body that steps down to a ½” diameter probe tip. The horn serves
as the second stage of acoustic amplification. It includes a removable or solid tip, a ½ - 20
stud for attachment to the convertor and external threads around the body for attachment
of accessories (e.g. Flow Cells). The Horn is available in three standard tip diameters of
½”, ¾” or 1". In addition, each horn comes in three different tip configurations. Tapped
horns have a replaceable Flat Tip; Solid horns do not have a replaceable tip; and Sapphire
tipped horns have a 1/16" thick sapphire disk bonded to the tip of a solid horn.
REPLACEABLE FLAT TIPS
A ¼” thick, radiating surface attached to the end of the horn. The flat tips will erode with use
and are replaceable. The tips are matched to horns of equal diameter (i.e. ½”, ¾” or 1"
diameter).

Operation Manual ____________________Function of Controls and Indicators
_________________________________________________________________________________________________
5-1
5. FUNCTION OF CONTROLS AND INDICATORS
XL2020 FUNCTIONS
POSITION
NO.
CONTROLS FUNCTION
1. Process Time Displays total pre-set time sonication is applied to sample
(1 sec to 10 hours maximum).
2. PULSARTM ON Time Indicates pulse ON time from 0.1 seconds to 1 hour.
3. Power Output Display Indicates relative percentage of maximum available
ultrasonic power delivered to the horn (i.e. 40% output = 0.4
x 600 Watts available = 240 Watts delivered).
4. PAUSE Indicator Indicates that unit is being temporarily interrupted during
processing.
5. TIMER Indicator Indicates that timer is set for processing.
6. CLEAR Key Clears previous or erroneous entries. Exits programming
mode.
7. ENTER Key Enters data during program mode.
8. Power Switch ON Position (I) - energizes generator.
OFF Position (O) - de-energizes generator.
9. LCD Display Screen Displays items 1, 2, 3, 10 & 11 listed below and prompts.
10. Elapsed Time Display Displays actual elapsed time of sonication.
11. PULSARTM OFF Time Indicates pulse OFF time from 0.1 seconds to 1 hour.
12. Overload Indicator Indicates a load condition greater than the maximum output
power of the generator or that the unit requires tuning or
other readjustments.
13. PULSARTM Indicator Indicates application of pulsed energy.
14. PAUSE Key Interrupts or Resumes processing cycle.
15. TUNE Key Activates or Deactivates tuning circuit.
16. PROG/DATA Key Initiates Timer and PULSARTM entry program and displays
entries on the LCD screen during run.
17. START/STOP Key Starts or Stops output in all modes of operation. Resets
timer in timed mode.
18. Amplitude Control Knob Controls the amplitude of ultrasonic vibration at the tip of
the horn.
19. Tuning Control Knob Allows adjustment of generator frequency to optimize
performance of convertor/horn assembly. Also used to
verify condition of horn and/or tip.
20. SHV Cable Connector Connects convertor cable to generator.
21. Footswitch Jack Connects external ON/OFF control (Foot Switch or Timer).
22. Power Receptacle Connects line cord to generator.
23. Fuse (10 amp 115V)
( 8 amp 220V)
Protects against electrical overload.

Operation Manual ____________________Function of Controls and Indicators
_________________________________________________________________________________________________
5-3
XL2010 & XL2015 FUNCTIONS
POSITION
NO.
CONTROLS FUNCTION
1. Overload Indicator Indicates a load condition greater than the maximum output
power of the generator or that the unit requires tuning or
other readjustments.
2. Tuning Switch Activates tuning circuit.
3. Power Switch ON Position (I) – energizes generator.
OFF Position (O) – de-energizes generator.
4. Power Output Display Indicates a RELATIVE percentage of maximum available
ultrasonic power delivered to the horn (i.e. 40% output = 0.4
x 550 Watts available = 220 Watts delivered).
5. PULSARTM Cycle Timer
(XL2015 Only)
Activates pulsed energy. Pulse width adjusts from 0.05 of a
second (5% duty) to 0.95 of a second (95% duty) every
second.
6. Amplitude Control Knob Controls the amplitude of ultrasonic vibration at the tip of
the horn.
7. Tuning Control Knob Allows adjustment of generator frequency to optimize
performance of convertor/horn assembly. Also used to
verify condition of horn and/or tip.
8. SHV Cable Connector Connects convertor cable to generator.
9. Footswitch Jack Connects external ON/OFF control (Foot Switch or Timer).
10. Power Receptacle Connects line cord to generator.
11. Fuse (10 amp 115V)
( 8 amp 220V)
Protects against electrical overload.

Operation Manual ________________________________Preparation for Use
_________________________________________________________________________________________________
6-1
6. PREPARATION FOR USE
1. If the Ultrasonic Processor has been left in a very cold environment for a prolonged
period of time, do not operate the unit until it has reached room temperature. Turn
the power switch ON, with the Amplitude Control Knob at zero, for 20 minutes before
using, to warm-up the electronics faster.
2. To safeguard the fuse against failure, always switch the power supply off before
connecting or disconnecting the foot switch plug.
3. Check to make sure that the Power Switch on the generator is turned OFF.
4. Turn the Amplitude Control Knob counter clockwise to setting zero.
5. Clean the mating surfaces of the convertor and probe or stepped microtip as well as
the threaded stud and hole. Check that the stud is tight. Do NOT apply grease to
mating surfaces or threads of the convertor, horn, replaceable flat tip or microtip
probe.
6. Connect the Power Cord to the receptacle on the rear of the generator and plug it
into a grounded power outlet.
7. The horn and its flat tip were assembled and attached to the convertor at the factory,
but it is recommended that you check the tightness of the horn and flat tip by using
the wrenches provided and referring to 6-3 & 6-4 of this manual. An insufficiently
tightened horn or tip will inhibit tuning and might cause damage to the generator
circuitry or mating parts of the convertor and horn. NEVER assemble or
disassemble the probe by holding the convertor in a vise. ALWAYS use the
wrenches supplied with the unit.
8. Mount the convertor and horn into the sleeve on the top of the SONABOXTM Acoustic
Enclosure (Part # 432B) or use the Convertor Stand and Positioner (Part # 438) to
hold the convertor and horn in position. Do NOT hold or clamp the convertor by the
front driver (black portion) or by the horn itself. Support the convertor only by
clamping around the broad (chrome) section. (See 6-2)
9. Before operation, be sure to tune the system. Refer to the tuning instructions, 7-1
through 7-3, of this manual. CAUTION! Improper tuning can cause damage to the
generator circuitry.
10. Connect either end of the coaxial HIGH FREQUENCY cable to the rear of the
chassis and the other end to the receptacle on the convertor. Push the connectors in
and turn the chrome rings clockwise ¼ turn to secure the connectors.
11. If a foot switch or timer switch is used, plug it into the FOOT SWITCH CONNECTOR.
Make sure that the plug is inserted properly.
12. If you will be using a MICROTIP or Extender, remove the Flat Tip on the end of the
horn, then attach the MICROTIP or Extender in its place. (See 6-3 & 6-4)

Operation Manual ________________________________Preparation for Use
_________________________________________________________________________________________________
6-3
REMOVAL AND ATTACHMENT OF HORNS AND TIPS
To connect a horn to the convertor or a tip to a horn:
1. Thread by hand until all joints mate smoothly.
2. Tighten using wrenches as explained below, being sure to apply even and firm
pressure.
3. If resistance is met, avoid cross threading and rethread by hand.
4. Do NOT force the threads by wrenching.
To attach or remove a horn:
1. Insert the pin of a pin spanner wrench into one of the holes at the base of the horn.
2. Insert the pin of a second pin spanner wrench into one of the holes in the front driver
(black portion) of the convertor.
3. Apply even and firm pressure.
To attach or remove a Flat Tip, MICROTIP or Extender:
1. Insert the pin of a pin spanner wrench into one of the holes at the base of the horn.
2. Place the opening of the 7/16" open end wrench on the wrench flats of the Flat Tip,
MICROTIP or Extender.
3. Apply even and firm pressure.
CAUTION!!
An insufficiently tightened horn, probe or tip will inhibit tuning and might cause damage to
the generator circuitry or mating parts of the convertor and horn. NEVER assemble or
disassemble the probe by holding the convertor in a vise. Always use the tools provided
and place a firm mat or towel underneath the convertor and horn to prevent slippage or
damage. Avoid pressing the sides of a MICROTIP or Extender down into a counter top
when tightening or removing.
A loud, high piercing sound emanating from the horn or probe indicates that one of the
components is loose or possibly cracked. STOP processing immediately. Turn off the
generator and check the tightness of the horn to the convertor, and the tip or probe to the
horn if applicable. Retune the generator and try running the unit again. If the loud noise
persists, or if you have difficulty tuning the unit, then contact MISONIX for assistance.

Operation Manual _________________________________Tuning Instructions
_________________________________________________________________________________________________
7-1
7. TUNING INSTRUCTIONS
XL2020 TUNING INSTRUCTIONS (PROBES & HORNS)
To assure proper operation, the generator must be tuned in accordance to the following procedure
each time a new probe is changed.
NOTE
I. The probe or microtip should not be immersed in the liquid or come in contact with the
work surface when tuning.
II. When operating with liquids at extreme temperatures, immerse the probe in the liquid for
a few minutes, remove from the liquid and then perform the tuning procedure.
III. See 7-3 for special tuning instructions for MICROTIPSTM, EXTENDERS, CUP HORNS and
TRAY HORNS.
1. Turn Amplitude Control Knob (see 5-2) fully counter-clockwise to zero.
2. Press Power Switch to the ON position. The switch should be illuminated.
3. Wait until the prompt “For Tuning Procedure, Refer to Manual” appears on the LCD display, then
press the TUNE key, and the LCD will display as follows:
Tuning - - - Probe Active .
4. Turn the Amplitude Control Knob slowly towards setting 3.
4.1. Pay strong attention to the bar graph of the LCD, STOP turning the Amplitude
Control Knob if the bar graph exceeds 70%.
4.2. Rotate the Tuning Knob (see 5-2) either clockwise or counter-clockwise until the
lowest possible bar graph reading (usually below 20%) is achieved.
5. Turn the Amplitude Control Knob slowly towards setting 6 and repeat steps 4.1 and 4.2.
6. Turn the Amplitude Control Knob slowly towards setting 10 and repeat steps 4.1 and 4.2.
7. Rotate knob toward setting 0 and observe that the bar graph is below 20% in all settings.
8. Press the TUNE key to end the tuning cycle.
9. Now the SONICATOR®is ready to use.

Operation Manual _________________________________Tuning Instructions
_________________________________________________________________________________________________
7-2
XL2010 & XL2015 TUNING INSTRUCTIONS (PROBES & HORNS)
To assure proper operation, the generator must be tuned in accordance to the following procedure
each time a new probe is changed.
NOTE
I. The probe or microtip should not be immersed in the liquid or come in contact with the
work surface when tuning.
II. When operating with liquids at extreme temperatures, immerse the probe in the liquid for
a few minutes, remove from the liquid and then perform the tuning procedure.
III. See 7-3 for special tuning instructions for MICROTIPSTM, EXTENDERS, CUP HORNS and
TRAY HORNS.
1. Turn Amplitude Control Knob (see 5-4) fully counter-clockwise to zero and turn Pulsar Duty
cycle to off for the Model XL2015.
2. Press Power Switch to the ON position. The switch should be illuminated.
3. Press and hold down the TUNE switch.
4. Turn the Amplitude Control Knob slowly towards setting 3.
4.1. Pay strong attention to the needle of the analog power meter, STOP turning the
Amplitude Control Knob if the bar graph exceeds 70%.
4.2. Rotate the Tuning Knob (see 5-4) either clockwise or counter-clockwise until the
lowest possible power meter reading (usually below 20%) is achieved.
5. Turn the Amplitude Control Knob slowly towards setting 6 and repeat steps 4.1 and 4.2.
6. Turn the Amplitude Control Knob slowly towards setting 10 and repeat steps 4.1 and 4.2.
7. Rotate knob toward setting 0 and observe that the bar graph is below 20% in all settings.
8. Release the TUNE switch.
9. Now the SONICATOR®is ready to use.

Operation Manual _________________________________Tuning Instructions
_________________________________________________________________________________________________
7-3
MICROTIP™ AND CUP HORN TUNING INSTRUCTIONS
MICROTIPSTM & EXTENDERS
1. Tuning of the horn should be done as quickly as possible. Running in air for a long
period of time may damage the horn.
2. Follow steps 1 through 3 in 7-1 for XL2020 or 7-2 for XL2010 & XL2015
3. Turn the Amplitude Control Knob (see 5-2 or 5-4) slowly towards setting 1.
3.1. Pay strong attention to the bar graph of the LCD on the XL2020 or the needle
of the analog power meter on the XL2010 & XL2015. STOP turning the
Amplitude Control Knob if the reading exceeds 70%.
3.2. Rotate the Tuning Knob (see 5-2 or 5-4) either clockwise or counter-
clockwise until the lowest possible reading (usually below 20%) is
achieved.
4. Turn the Amplitude Control Knob slowly towards setting 3 and repeat steps 3.1 and
3.2.
5. Turn the Amplitude Control Knob slowly towards setting 5 and repeat steps 3.1 and
3.2.
6. Follow steps 7 through 9 in 7-1 for XL2020 or 7-2 for XL2010 & XL2015.
CUP HORNS & TRAY HORNS
1. Do not tune horn with liquid in the cup. Drain filled cup down to the outlet fitting level,
perform the tuning procedure as outlined in 7-1 for XL2020 or 7-2 for XL2010 &
XL2015, then refill the liquid to the desired level.

Operation Manual _____________Programming the Microprocessor of XL2020
_________________________________________________________________________________________________
8-1
8. PROGRAMMING THE MICROPROCESSOR OF XL2020
REVIEW OF KEYS
Key Action
TUNE Activates or Deactivates tuning circuit.
START
STOP
Starts or Stops output in all modes of operation. Resets
timer in timed mode.
PROG.
DATA
Initiates Timer and PULSARTM entry program and displays
entries on the LCD screen during run.
ENTER Enters data during program mode.
CLEAR Clears previous or erroneous entries. Exits programming
mode.
PAUSE Interrupts or Resumes processing cycle.
60 70 9080 100%401003020 50
TM

Operation Manual _____________Programming the Microprocessor of XL2020
_________________________________________________________________________________________________
8-2
CONTINUOUS MODE
Ultrasonic energy is delivered continuously without any timing or pulsing cycles. The
ultrasonic output will shut down when the total processing time reaches 9:59:59
(hr:min:sec).
Programming Steps and Descriptions Action LCD Display
Turn ON generator. Press “I” on Power
Switch
SONICATOR XL2020
MISONIX INCORPORATED
(Scrolls on screen, then switches to)
For Tuning Procedure
Refer to Manual
(alternates with)
If SONICATOR is Tuned,
Press CLEAR
Clear introduction message. Press CLEAR Key
Press PROG to Program
Timer and PULSAR or
(alternates with)
Press START for
Continuous Operation
Start sonication in continuous mode.
(Turn Amplitude Control Knob to desired
level)
Press START Key
STOP
Continuous 0:00:00
(Elapsed Time begins to count seconds)
To interrupt processing. Press PAUSE Key Continuous X:XX:XX
Press PAUSE to Continue
To continue processing. Press PAUSE Key Continuous X:XX:XX
(Timer continues to count seconds)
Stop sonication when finished. Press START Key
STOP
Press PROG to Program
Timer and PULSAR or
(alternates with)
Press START for
Continuous Operation

Operation Manual _____________Programming the Microprocessor of XL2020
_________________________________________________________________________________________________
8-2
TIMER MODE ONLY
Ultrasonic energy is delivered continuously for a specified length of time up to 9:59:59
(hr:min:sec).
Programming Steps and Descriptions Action LCD Display
Turn ON generator. Press “I” on Power
Switch
SONICATOR XL2020
MISONIX INCORPORATED
(Scrolls on screen, then switches to)
For Tuning Procedure
Refer to Manual
(alternates with)
If SONICATOR is Tuned,
Press CLEAR
Clear introduction message. Press CLEAR Key
Press PROG to Program
Timer and PULSAR or
(alternates with)
Press START for
Continuous Operation
Begin programming. Press PROG. Key
DATA
0:00:00 PROG, START, CLEAR
00:00.0 (MEM.0) 00:00.0
Select the default memory location
(MEM.0)
Press PROG. Key
DATA
0:00:00 (hr:min:sec)
Enter Total Process Time
(alternates with)
0:00:00 (hr:min:sec)
Then Press ENTER
Enter total processing time from
00:00:01 to 9:59:59.
For example: 20 minutes
Press [2] [0] [0]
[0] Keys, and then
press ENTER Key
Enter Pulse ON Time
00:00.0 (min:sec.tenths)
(alternates with)
Then Press ENTER
00:00.0 (min:sec.tenths)
Skip pulsing feature.
Enter 0 for pulse ON time.
Press [0] Key, and
then press ENTER
Key
0:20:00 PROG, START, CLEAR
00:00.0 (MEM.0) 00:00.0
Start sonication in timed mode.
(Turn Amplitude Control Knob to desired
level)
Press START Key
STOP
0:20:00 0:00:00
(Elapsed Time begins to count seconds)
To interrupt processing. Press PAUSE Key 0:20:00 X:XX:XX
Press PAUSE to Continue
To continue processing. Press PAUSE Key 0:20:00 X:XX:XX
(Timer continues to count seconds)
To display parameters of program. Press PROG. Key
DATA
0:20:00 hr:mn:sc X:XX:XX
00:00.0 min:sec 00:00.0
(Then switches back to)
0:20:00 X:XX:XX
(Timer is always counting seconds)
To abort program. Press START Key
STOP
0:20:00 PROG, START, CLEAR
00:00.0 (MEM.0) 00:00.0
When program is completed, exit
programming mode.
Press CLEAR Key Press PROG to Program
Timer and PULSAR or
(alternates with)
Press START for
Continuous Operation

Operation Manual _____________Programming the Microprocessor of XL2020
_________________________________________________________________________________________________
8-4
PULSARTM MODE ONLY
Ultrasonic energy is delivered in pulsed cycles (programmable ON and OFF times), but
without a specified total processing time. The range of pulse ON and OFF times can be
entered from 00:00.1 to 59:59.1 (min:sec.tenths). Ultrasonic output will shut down when the
total processing time reaches 9:59:59 (hr:min:sec).
Programming Steps and Descriptions Action LCD Display
Turn ON generator. Press “I” on Power
Switch
SONICATOR XL2020
MISONIX INCORPORATED
(Scrolls on screen, then switches to)
For Tuning Procedure
Refer to Manual
(alternates with)
If SONICATOR is Tuned,
Press CLEAR
Clear introduction message. Press CLEAR Key
Press PROG to Program
Timer and PULSAR or
(alternates with)
Press START for
Continuous Operation
Begin programming. Press PROG. Key
DATA
0:00:00 PROG, START, CLEAR
00:00.0 (MEM.0) 00:00.0
Select the default memory location
(MEM.0)
Press PROG. Key
DATA
0:00:00 (hr:min:sec)
Enter Total Process Time
(alternates with)
0:00:00 (hr:min:sec)
Then Press ENTER
Skip timer feature.
Enter 0 for total process time.
Press [0] Key, and
then press ENTER
Key
Enter Pulse ON Time
00:00.0 (min:sec.tenths)
(alternates with)
Then Press ENTER
00:00.0 (min:sec.tenths)
Enter pulse ON time from 00:00:00.1 to
59:59:00.1.
For example: 30 seconds
Press [3] [0] [0]
Keys, and then
press ENTER Key
Enter Pulse OFF Time
(min:sec.tenths) 00:00.0
(alternates with)
Then Press ENTER
(min:sec.tenths) 00:00.0
Enter pulse OFF time from 00:00:00.1 to
59:59:00.1.
For example: 7.5 seconds
Press [7] [5] Keys,
and then press
ENTER Key
0:00:00 PROG, START, CLEAR
00:30.0 (MEM.0) 00:07.5
Start sonication in pulsed mode.
(Turn Amplitude Control Knob to desired
level)
Press START Key
STOP
Pulsing 0:00:00
(Elapsed Time begins to count seconds)
To interrupt processing. Press PAUSE Key X:XX:XX
Press PAUSE to Continue
To continue processing. Press PAUSE Key X:XX:XX
(Timer continues to count seconds)
To display parameters of program. Press PROG. Key
DATA
0:00:00 hr:mn:sc X:XX:XX
00:30.0 min:sec 00:07.5
(Then switches back to)
X:XX:XX
(Timer is always counting seconds)
To abort program. Press START Key
STOP
0:00:00 PROG, START, CLEAR
00:30.0 (MEM.0) 00:07.5
When program is completed, exit Press CLEAR Key Press PROG to Program

Operation Manual _____________Programming the Microprocessor of XL2020
_________________________________________________________________________________________________
8-4
programming mode. Timer and PULSAR or
(alternates with)
Press START for
Continuous Operation
TIMER AND PULSARTM MODE
Ultrasonic energy is delivered in pulsed cycles (programmable ON and OFF times), for a
specified total processing time. The range of pulse ON and OFF times can be entered from
00:00.1 to 59:59.1 (min:sec.tenths), with a maximum total processing time of 9:59:59
(hr:min:sec).
Programming Steps and Descriptions Action LCD Display
Turn ON generator. Press “I” on Power
Switch
SONICATOR XL2020
MISONIX INCORPORATED
(Scrolls on screen, then switches to)
For Tuning Procedure
Refer to Manual
(alternates with)
If SONICATOR is Tuned,
Press CLEAR
Clear introduction message. Press CLEAR Key
Press PROG to Program
Timer and PULSAR or
(alternates with)
Press START for
Continuous Operation
Begin programming. Press PROG. Key
DATA
0:00:00 PROG, START, CLEAR
00:00.0 (MEM.0) 00:00.0
Select the default memory location
(MEM.0)
Press PROG. Key
DATA
0:00:00 (hr:min:sec)
Enter Total Process Time
(alternates with)
0:00:00 (hr:min:sec)
Then Press ENTER
Enter total processing time from
00:00:01 to 9:59:59.
For example: 10 minutes
Press [1] [0] [0]
[0] Keys, and then
press ENTER Key
Enter Pulse ON Time
00:00.0 (min:sec.tenths)
(alternates with)
Then Press ENTER
00:00.0 (min:sec.tenths)
Enter pulse ON time from 00:00:00.1 to
59:59:00.1.
For example: 1 second
Press [1] [0] Keys,
and then press
ENTER Key
Enter Pulse OFF Time
(min:sec.tenths) 00:00.0
(alternates with)
Then Press ENTER
(min:sec.tenths) 00:00.0
Enter pulse OFF time from 00:00:00.1 to
59:59:00.1.
For example: 1.5 seconds
Press [1] [5] Keys,
and then press
ENTER Key
0:10:00 PROG, START, CLEAR
00:01.0 (MEM.0) 00:01.5
Start sonication in timed and pulsed
mode. (Turn Amplitude Control Knob to
desired level)
Press START Key
STOP
0:10:00 Pulsing 0:00:00
(Elapsed Time begins to count seconds)
To interrupt processing. Press PAUSE Key 0:10:00 Pulsing X:XX:XX
Press PAUSE to Continue
To continue processing. Press PAUSE Key 0:10:00 Pulsing X:XX:XX
(Timer continues to count seconds)
To display parameters of program. Press PROG. Key
DATA
0:10:00 hr:mn:sc X:XX:XX
00:01.0 min:sec 00:01.5
(Then switches back to)