Labnet Labpette R User manual

1. Components and functions
(1) Plunger
Depressing the plunger dispenses the set volume of liquid
(2) Volume adjustment dial
Select position 1 to 5 to set desired volume (see table 1)
(3) Fill lever
Fills tip with liquid when pushed upwards
(4) Locking lever
Secures reservoir tip in place
(5) Reservoir tip
Reservoir for holding liquid to be dispensed. For best
results use Labnet Combi-Syringes.
2. Selecting the volume
•Locate the volume to be dispensed in table 1
•Set the volume adjustment dial to the position
indicated at the top of the column.
•Choose the appropriate reservoir tip from the left
hand column of the chart.
•The maximum number of dispensing steps for the
selected tip and volume is located at the bottom of
the column.
3. Inserting reservoir tip
• Push the fill lever down until it stops.
• Push the locking lever upwards.
• Insert a reservoir tip with slight pressure to lock it in
place.
• Push the fill lever down slightly and press down on
the locking lever to secure the tip in place.
•Use the volume adjustment dial to set the required
volume.
Labpette R
Instruction Manual
Table 1
Dial position 12345
using 0.05mltip 1µl2µl3µl4µl5µl
using 0.50mltip 10µl 20µl 30µl40µl50µl
using 1.25mltip 25µl 50µl 75µl 100µl 125µl
using 2.50mltip 50µl 100µl 150µl 200µl 250µl
using 5.00mltip 100µl 200µl 300µl 400µl 500µl
using12.50mltip 250µl 500µl 750µl 1000µl 1250µl
using25.00mltip 500µl 1000µl 1500µl 2000µl 2500µl
using50.00mltip 1000µl 2000µl 3000µl 4000µl 5000µl
Steps 48 23 15 11 8
Please note :
25 ml und 50 ml tips require the use of an
adapter (adapters are supplied free with
each Labpette R repeating pipettors).
Attach the adapter by placing it on the tip
as shown in the illustration and turning
clockwise. After the tip has been used,
the adapter may be removed and reused.
0,05 ml tips should be used with a 10 µl
pipette tip on the liquid end.
4. Filling the reservoir tip
•Immerse the end of the reservoir tip into the liquid to
be drawn up.
•Slowly raise the fill lever upwards to draw liquid into
the tip.
• Dispense the first step with the plunger.
The fill lever must be raised slowly to
prevent negative pressure and air
bubbles in the liquid.
A small air bubble in the upper part of
the reservoir will not affect the
accuracy as the last step is not
carried out.
The reservoir tip does not need to be
filled completely.
The 0.5 ml, l, 1.25 ml and 2.5 ml tips
may be used with a 100 µl pipette tip
affixed to the end. This facilitates filling
from vessels with narrow necks and
provides better accuracy with small
volumes.
5. Dispensing
•Check that the volume setting is correct.
•Wipe the end of the tip with a lint free wipe or other
appropriate material.
•Place the tip at an angle to the wall of the vessel.
Press the plunger down to the stop to dispense.
After using the 25 ml and 50 ml
tips, the adapter can be removed
by turning it counter-clockwise and
reused with another tip.
Avoid touching the end of the tip
when removing it from the pipette.
When using viscous solutions,
the fit of the tip may loosen after
several pipettings.

6. Removing the tip
•Empty the tip completely.
•Push the locking lever upwards.
•Remove the tip from the dispenser.
7. Maintenance
Labpette R repeating pipettors requires no
maintenance. Potential clogs are best removed
with warm water or isopropanol.
Do not attempt to disassemble the
Labpette R repeating pipettor. Doing so will void
the warranty.
8.
Troubleshooting
Problem Possible cause Remedy
Tips will not fit in pipette Fill lever has not been pushed down completely Push fill lever down to the
stop
Locking lever cannot be pushed down Tip has not been inserted Properly Remove the tip and push
tip piston in. Be sure the
filling lever is in the down
position, replace tip and
press locking lever down
again
Fill lever cannot be moved upwards Locking lever has not been pushed Push the locking lever
down completely down to the stop
Dispensed volume is not correct Volume adjustment wheel is not locked in place Lock adjustment wheel in
place
9. Technical data
Weight : Approx. 100 grams
Accuracy and precision : see Table 2
Liquid : bidistilled water
Reference temperature : 20° - 25° C
±0.5° C constant
Measurement : according to DIN 12650
Subject to technical modifications
Made in Germany
Table 2
Dial position 1 2 3 4 5 Inpre- Inaccur-
Steps 48 23 15 11 8 cision (%) acy(%)
using 0.05 ml tip 1 µl2µl3µl4µl5µl<5 <±2,5
using 0.50 ml tip 10 µl 20 µl 30 µl 40 µl 50 µl < 0,7 < ±0,8
using 1.25 ml tip 25 µl 50 µl 75 µl 100 µl 125 µl < 0,5 < ±0,8
using 2.50 ml tip 50 µl 100 µl 150 µl 200 µl 250 µl < 0,4 < ±0,8
using 5.00 ml tip 100 µl 200 µl 300 µl 400 µl 500 µl < 0,3 < ±0,4
using 12.50 ml tip 250 µl 500 µl 750 µl 1000 µl 1250 µl < 0,3 < ±0,3
using 25.00 ml tip 500 µl 1000 µl 1500 µl 2000 µl 2500 µl < 0,3 < ±0,2
using 50.00 ml tip 1000 µl 2000 µl 3000 µl 4000 µl 5000 µl < 0,2 < ±0,2
10.Ordering information
Labnet Combi-Syringe tips may be ordered
from your Labnet dealer. All sizes are supplied
in stackable dispenser boxes and are available
in sterile or non-sterile packaging.
11.Warranty
Labpette R repeating pipettors are guaranteed
against defects in materials and workmanship
for a period of 6 months from the data of
purchase. This warranty is valid only if the
dispenser has been used in compliance with
these instructions. If a failure cannot be
remedied using the troubleshooting guide
above, contact Labnet for further assistance. P.O. Box 841 • Woodbridge, NJ 07095 • Phone: 888-LABNET1 or 732 417-0700
Fax: [phone] • email: [email protected] • http://www.labnetlink.com