Amos AEM 480 User manual

AEM 480 （Version：AMOSAEM480OP20180306）
Fully-automatic Microtome
Operation Manual
www.amos-scientific.com
AMOS SCIENTIFIC PTY.LTD. ABN37 159 778 140

This Operation manual introduces the instruments component, key
features and using notes. Be sure to read these instructions before using
the instrument.
Smooth-turning
handwheel
Lever for activating the
handwheel brake
Handwheel Locking
Mechanism
Knife holder
Section Waste tray
Separate control panel
Display Panel

Foreward
AEM480 Motorized Microtome is
designed for the worldwide user. It can meet
the different requirement in the fields of
biology, medicine and industry.
It is necessary to read this technical
manual to ensure that the instrument is snuff,
long haul and safely used.
Our company is responsible for service
after the microtome have been used, our
agents have ability to service, the client all of
world can connect with our agent in order to
get timely service.
Attention: our company products
will be mended and updated continually
along with developing of the technology.
Regarding of being updated of the
technical data and instruments
component, we will not inform.

Contents
1、Safety Notes………………………………………………………1
1. 1 O v er v ie w …… …… … … …… …… … … …… … …… … …… … … …… …… 1
1.2 Safety Warning……………………………………………………………..1
1.3 Safety Devices………………………………………………………………3
2、Performance & Parameters……………………………………..4
2.1 Overview-Instrument Components……………………………………… .4
2.2 Performance Index…………………………………………………………5
2.3 Technical Data………………………………………………………………5
3、Preparing before using…………………………………………..6
3.1 Installation Site Requirement…………………………………………… ...6
3.2 Standard Delivery………………………………………………………… ..6
3.3 Ins tall ation……… ……………………… ………………………………… ..7
3.4 Electrical Connection……………………………………………………….8
4、Operation…………………………………………………………9
4.1 Control Panel Function &Control…………………………………………9
4.2 Knife Holder inserting…………………………………………………… ..13
4.3 Clamping The Specimen………………………………………………… ...14
4.4 Specimen Clamps/Knife Inserting……………………………………… ...15
4.5 Trimming ……………………………………………………………………1 6
4.6 Sectioning…………………………………………………………………...18
5、Clearance & Maintenance………………………………………19
5.1 Cleaning The Instrument………………………………………………… ..19
5.2 Maintenance……………………………………………………………… ...21
6、Trouble Shooting…………………………………………………22
7、Instrument Diagram……………………………………………..25

AEM480 Fully-automatic Microtome
AMOSAEM480OP20180306
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1、Safety Notes
1.1 Overview
The Instruction Manual contains important safety instructions and information. The operation
manual is an important part of the instrument, which the operator must be read carefully prior to
startup and use in order to running safely. It will at a critical moment to ensure personal safety
and avoid instrument damage. Please keep the Manual near the instrument to easy read.
This instrument was built and test in accordance with the safety regulations as specified below:
GB4793.1-2007 Medical Electrical Equipment First Part: Current Requirements for safety.
▲ Notes：The safety marks and protective device on both instrument and accessories may neither
be removed nor modified, so as to not injury body or instrument.
1.2 Safety Warning
The following safety notes relating to transport, installation, calibration, operation, maintenance,
cleaning and all aspects of the instrument. Every principal person must be read carefully and
carry out this mentioned strictly.
1.2.1 Warnings-Transport and Installation
● The instrument may only be transported or moved in an upright position always, the
tilt angle should not exceed 45℃!
● After installation, please remove the section waste tray and knife holder before
transporting or movement!
● The input voltage has been preset at the factory, please check this setting complies
with your local power requirement before connecting the instrument to the mains
power!
● Please using the power cord provided. If changing the power cord, must ensure the
power cord with earth wire!
● Don’t operate in rooms with explosion hazard !
● The protective devices on both instrument and accessories must neither be removed
nor modified, in order to avoid injury to instrument or person body !

AEM480 Fully-automatic Microtome
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1.2.2 Warnings-Operation
● Take care when handling microtome knives and disposable blades. The cutting edge
is extremely sharp and can cause serious injury!
● Always remove the knife/blade before detaching the knife holder from the instrument,
and put the knives back into the knife box when not in use!
● Never place knife anywhere with the cutting edge facing upwards and never try to
catch knife when you take out the knife!
● Always clamp the specimen block before clamping the knife!
● Prior to changing the specimen and knife, always lock the handwheel firstly. If
changing the specimen only, always must cover the cutting edge with the knife guard !
● Always turn the handwheel clockwise, otherwise , the effect may affect the slice!
● Always cover the knife guard when slicing!
● Don’t Clockwise and Counterclockwise rotate the handwheel frequently at top and
bottom! Slice thickness many deviations!
● Ensure that liquids don’t enter the interior of the instrument during working!
● Don’t touch the handwheel while it is running, otherwire injury the operator!
1.2.3 Warning- Maintenance and Cleaning
● Only authorized person may do the service and repair!
● Before cleaning, always switch off the instrument, disconnect the power plug,
remove the knife holder completely and clean it separately. Must remove the blade
before cleaning the knife holder!
● Lock the handwheel before each cleaning!
● Don’t use any solvents containing acetone or xylene for cleaning!
● Ensure that no liquids enter the interior of the instrument when cleaning!
● Don’t turn on the instrument before it is completely dry!
● Turn the instrument off with the mains switch and pull the mains plug before
replacing the fuses! Only use fuses of the same specification and operation in this
manual.

AEM480 Fully-automatic Microtome
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1.3 Safety Devices
Handwheel locking mechanism
As showed in the Fig, lever（1）and（2）
can be to lock the handwheel.
To clockwise push lever（1）until it lock the
handwheel while specimen place at the top
position.
And also can clockwise rotate lever（2）about
180°which it may lock handwheel in any
position.
Rotate the lever counterclockwise to unlock
the handwheel.
The Left drawing shows the status at :
Lever (1) is activated to lock and lever (2) is
not activated.
When the lock is activated, the “LOCK”
indicator will light on display panel while the
machine DON’T running.
●DON’T lock the handwheel when the
handwheel is rotating, otherwise it will damage
the instrument.
▲Attention：Before remove the instrument, change specimen and blade or cleaning and
maintenance the instrument, the handwheel must be locked.
Knife Guard on the knife holder
Turn the knife guard(3) upward to cover
the knife to avoid personal injury and damage
the knife edge.
The knife guard（3）on the upper station
(see the drawing) that is stay in protection.
●Note: Only authorized and qualified service
personnel may access the internal components
of the instrument.
Emergency Stop Function
Fig（4）is switch for Emergency Stop.
The emergency-stop function is activated with
the red switch(4) pressed while the main power
off and all actions stops.
To deactivate this function, turn the switch in
the direction of the arrow.
The function should be use on emergency status.
Correct use in order:
1
2
3
4

AEM480 Fully-automatic Microtome
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Activate Emergency switch —turn off power
switch—deactivate—turn on power switch.
2、Performance & Parameters
2.1 Overview- Instrument Structure
Handwheel
Instrument Base
Handle of handwheel
Storage Tray
Power Switch
Separate Control Panel
connection with socket
Power Socket
with fuse inside
Emergency-stop
Switch
Quick release
cassette clamp
Knife guard at knife holder
Knife holder
Section waste tray
Specimen clamp adjustor
Clamping lever for knife holder
Display panel
Separate control panel
Handwheel locking
mechanism-lock at
any position
Handwheel locking
Mechanism-lock at
fixed position

AEM480 Fully-automatic Microtome
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2.2 Performance Index
AEM480 is a motorized rotary microtome. The stepper motor operated the specimen
movement forward and back, coarse feed and sectioning in manual operation. It is better in
precision sectioning, easy operation.
⊙The crust is made in ABS plastic with fire prevention function, and streamlined
designed in artistic appearance.
⊙Spacious ingenious assembly section waste tray.
⊙Retraction function make it is better to sectioning. The retraction value can be set.
⊙Quick release cassette clamp and specimen clamp fit to internal Embedding
cassette.
⊙Locking may be activated at any position by handwheel. It ensure cassette
changing safety when the cassette located in the toppest locked.
⊙The electric coarse feed operates two speeds which is easy to record the feed.
⊙Separate control panel is more easy to operate.
⊙Three motorized sectioning modes (Cont, Single and Step) are available, user can
choose at will.
⊙Automatic memorized coarse feed and reload fast can improve efficiency during
sectioning.
2.3 Technical Data
⊙Surroundings requirements:
Working temperature：+10℃—40℃
Working humidity: <80%，defrosting
Working pressure:（86～106）kPa；
⊙Power supply：100-240 VAC±10 %
⊙Frequency：50/60 Hz
⊙Power：<60 VA
⊙Fuse：3 A
⊙Safe classify：ClassifyⅠ- type B
⊙Section thickness：0 to 600µm
0 to 2µm，0.5µm increments
2 to 10µm，1µm increment
10 to 20µm，2µm increments
20 to 100µm，5µm increments
100 to 600µm，50µm increments
⊙Trimming thickness: 0 to 600µm
0 to 2µm，0.5µm increments
2 to 10µm，1µm increment
10 to 20µm，2µm increments
20 to 100µm，5µm increments
100 to 600µm，50µm increments