MJ Research PTC-100 User manual

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PTC-100 Operations Manual
Version 8.1
MJ Research™, Incorporated
Boston • San Francisco • Tahoe • Copenhagen • Seoul
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Copyright ©1997, MJ Research™, Incorporated. All rights reserved.
P C-100, P C-100HB, 16MS Slide Block, Chill-out, Frame-Seal, Hot Bonnet, Microseal, Self-Seal,
Slide Griddle, MJ Research marks and helix logo are trade and/or service marks belonging to MJ
Research, Incorporated.
hermal cyclers can be used for a number of purposes, including the polymerase chain reaction
(PCR). PCR is covered by patents owned by Hoffmann-La Roche, Inc., and F. Hoffmann-La Roche,
Ltd., who have granted exclusive and nonexclusive licenses for some types of applications. Roche
and its licensees provide end-user licenses within their respective fields. hese licenses have dif-
ferent terms depending on the particular application of PCR, and different rules may apply in
different countries. Anyone who intends to use MJ RESEARCH equipment to do PCR is encouraged
to contact Hoffmann-La Roche for more information, at one of the addresses below:
In the United States: In other nations:
Licensing Manager PCR Licensing Manager
Roche Molecular Systems, Inc. F. Hoffmann-La Roche Ltd.
1145 Atlantic Avenue Building 222/350
Alameda, CA 94501 USA CH-4002 Basel, Switzerland
[phone] 41-61-687-3031
Fax: [phone] Fax: [phone]
06107-AA
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Contents
Documentation Conventions ............................................................................................... iv
1. Introduction ................................................................................................................. 1-1
2. Layout and Specifications .......................................................................................... 2-1
3. Installation.................................................................................................................... 3-1
4. Operation...................................................................................................................... 4-1
5. Running Protocols....................................................................................................... 5-1
6. Programming ............................................................................................................... 6-1
7. Editing Programs ........................................................................................................ 7-1
8. Maintenance ................................................................................................................. 8-1
9. roubleshooting ........................................................................................................... 9-1
Appendix A: he 16MS Slide Block ................................................................................ A-1
Appendix B: Safety Warnings .......................................................................................... B-1
Appendix C: Factory-installed Protocols ....................................................................... C-1
Appendix D: Warranties ................................................................................................... D-1
Appendix E: Shipping Instructions for US Residents .................................................. E-1
Index ..................................................................................................................................... In-1
Declaration of Conformity ............................................................................................... DoC-1
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Documentation Conventions
Typographic Conventions
he names of keyboard keys are in sans serif type and placed within
double angle brackets:
E ample: «Proceed»
Items in programming menus are italicized:
E ample: Select Edit from the Main Menu.
Graphic Conventions
he programming screens displayed in the LCD window are represented
by a box containing two lines of text:
E ample:
Terminology
A programming option is termed “selected” when the cursor is positioned
in front of it. Use the «Select» keys (see fig. 2-3) to move the cursor. In
some screens selected items are also displayed in all-capital letters.
RUN Enter
Program Program
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1-11-1
1-11-1
1-1
Introduction
1
Meet the PTC-100......................................... 1-2
How to Use This Manual .............................. 1-2
Important Safety Information ..................... 1-2
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1-21-2
1-21-2
1-2
PTC-100 Operations Manual
Meet the PTC-100
hank you for purchasing an MJ RESEARCH P C-100 thermal cycler. De-
signed by a team of molecular biologists and engineers, the P C-100 will
meet your needs for an easy-to-use, reliable, compact programmable ther-
mal cycler:
• Easy-to-read programming interface for quick and painless program-
ming, editing, file management, and much more
• Choice of block or probe control
• Space-saving design for easy setup and transportation
• Instant Incubate feature for continuous-temperature incubations
• Hot Bonnet heated lid for oil-free cycling
• Customizable factory-installed protocols
How to Use This Manual
his manual contains all the information you need to operate the P C-100
safely and productively:
• Chapter 2 describes the physical characteristics and specifications
of the P C-100.
• Chapters 3–5 describe installing and operating the P C-100.
• Chapters 6 and 7 describe programming the P C-100.
• Chapter 8 describes proper maintenance of the P C-100.
• Chapter 9 offers advice on troubleshooting the P C-100.
Note: Information on installing, operating, and programming the 16MS
Slide Block cycler may be found in appendix A.
Important Safety Information
Safe operation of the P C-100 begins with a complete understanding of
how the machine works. Please review this entire manual before attempt-
ing to operate the P C-100. Do not allow anyone who has not reviewed
this manual to operate the machine.
he P C-100 can generate enough heat to inflict serious burns and can
deliver strong electrical shocks if not used according to the instructions
in this manual. Please read the safety warnings and guidelines in appen-
dix B, and exercise all precautions outlined in them.
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2-12-1
2-12-1
2-1
Layout and Specifications
2
PTC-100 with Cold Lid .................................. 2-2
Models Available ..................................................... 2-2
Front View (Figure 2-1 ............................................. 2-2
Back View (Fig. 2-2 ................................................. 2-3
Control Panel (Fig. 2-3 ............................................ 2-3
PTC-100 with Hot Bonnet ............................. 2-4
Models Available ..................................................... 2-4
Front View (Fig. 2-4 ................................................. 2-4
Back View (Fig. 2-5 ................................................. 2-5
PTC-100 with 16MS Slide Block (Fig. 2-6) ...... 2-6
Accessories ................................................. 2-7
Temperature Probe (Fig. 2-7 .................................... 2-7
Slide Griddle Adapter (Fig. 2-8 ............................... 2-7
Specifications .............................................. 2-8
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2-22-2
2-22-2
2-2
PTC-100 Operations Manual
PTC-100 with Cold Lid
Front View (Fig. 2-1)
Models Available
60-well block: holds 60 x 0.5ml tubes
96-well block: holds 96 x 0.2ml tubes or one 96-well microplate
96Ag gold/silver block: holds 96 x 0.2ml tubes or one 96-well microplate; block is gold-
plated sterling silver for faster ramping speed
16MS Slide Block: holds sixteen 25 x 75mm slides and 24 x 0.2ml tubes (see p. 2-6)
Lid
Air exhaust vents
Control panel
Air intake vents
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2-32-3
2-3
Layout and Specifications
Back View (Fig. 2-2)
Control Panel (Fig. 2-3)
Air intake vents
Parallel printer port
Hot Bonnet jack (unused
in this model
Power cord jack
On-off switch
123
456
70
89
LCD window
Left and right
selection keys
Proceed key
Cancel key
LED bar graph
Chart recorder jack
Probe jack
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2-42-4
2-4
PTC-100 Operations Manual
Models Available
60-well block: holds 60 x 0.5ml or 0.6ml microcentrifuge tubes
96-well block: holds 96 x 0.2ml tubes or one 96-well microplate
96Ag gold/silver block: holds one 96-well microplate or 96 x 0.2ml tubes; block is gold-
plated sterling silver for faster ramping speed
Front View (Fig. 2-4)
PTC-100 with Hot Bonnet
Control panel
Hot Bonnet
Thumbwheel
Lid release button
Air exhaust vents
Air intake vents
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2-52-5
2-52-5
2-5
Layout and Specifications
Back View (Fig. 2-5)
Power cord jack
On-off switch
Air intake vents
Parallel printer port
Hot Bonnet jack (with
cord plugged in
Hot Bonnet cord
Chart recorder jack
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2-62-6
2-62-6
2-6
PTC-100 Operations Manual
PTC-100 with 16MS Slide Block
(Fig. 2-6)
Plastic pin
Slide block
Air exhaust vents
Control panel
Air intake vents
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2-72-7
2-72-7
2-7
Layout and Specifications
Accessories
Temperature Probe (Fig. 2-7)
Allows machine to control reactions according to a representative sample’s temperature. Avail-
able for the 60-well model of the P C-100, with or without the Hot Bonnet.
Slide Griddle Adapter (Fig. 2-8)
Allows machine to thermally cycle up to four 25 x 75mm glass slides. wo models are available:
the actively controlled adapter and the passive adapter. Available for 60-well and 96-well models
of the P C-100, with or without the Hot Bonnet.
Probe jack
Probe tube, with sensor inside
Active slide griddle Passive slide griddle
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2-82-8
2-82-8
2-8
PTC-100 Operations Manual
Specifications
Thermal range: 0–100°C
Accuracy: ±0.5°C of programmed target at 60°C
Thermal homogeneity: ±0.4°C well to well within 30 seconds of arrival at 60°C
16MS slide bloc : ±0.4°C within 30 seconds of arrival at 90°C
Ramping rate: 60-well block: up to 1°C/sec
96-well: up to 1.2°C/sec
96AgV gold/sil er block: up to 2.5°C/sec
16MS Slide Block: up to 0.5°C/sec
Sample capacity: 60-well block: Sixty x 0.5ml tubes
96-well block, 96AgV gold/sil er block: 96 x 0.2ml tubes or
one 96-well plate
16MS Slide Block: Sixteen 25 x 75mm slides and 24 x 0.2ml
tubes
Line oltage: 100–240VAC rms (no adjustment needed among voltages
within these ranges)
Frequency: 50/60Hz single phase
Power: 350W maximum (momentary 4.5 A)
Fuses: Two 4A, 5 x 20mm (4 ampere)
Displays: One 2 x 16 LCD alphanumeric display
Ports: One 25-pin 8-bit parallel interface printer port
Memory: 360 typical programs, in nonvolatile memory; programs may
contain up to 100 steps; maximum of 1600 steps among all
programs
Weight: 7.0 g
Size: PTC-100 with cold lid, 24cm x 28cm x 23cm; PTC-100 with
Hot Bonnet, 23cm x 28cm x 26cm
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3-13-1
3-13-1
3-1
Installation
3
Packing List ................................................. 3-2
Setting Up the PTC-100 ................................ 3-2
Environmental Requirements....................... 3-2
Power Supply Requirements ....................... 3-3
Air Supply Requirements ............................. 3-3
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3-23-2
3-23-2
3-2
PTC-100 Operations Manual
Packing List
After unpacking the P C-100, make sure you have received the following:
• One P C-100, with Hot Bonnet if ordered
• One power cord
• wo fuses
• One sponge rubber pad (with 96-well block, for cold lid machines
only)
•The PTC-100 Operations Manual (this document)
If any of these components are missing or damaged, contact MJ RESEARCH
or the authorized distributor from whom you purchased the P C-100 to
obtain a replacement. Please save the original packing materials in case
you need to return the P C-100 for service. See appendix E for shipping
instructions.
Setting Up the PTC-100
Insert the power cord plug into its three-pronged jack at the back of the
machine (see fig. 2-2), then plug the cord into an electrical outlet (see
“Power Supply Requirements,” below).
For the P C-100 with Hot Bonnet, screw the Hot Bonnet power cord into its
jack at the back of the machine (see fig. 2-5; finger-tightness is adequate).
Situate the machine according to the instructions below.
Environmental Requirements
Ensure that the area where the P C-100 is installed meets the following
conditions, for reasons of safety and performance:
• Indoor use, nonexplosive environment
• Normal air pressure (altitude below 2000m)
• Ambient temperature 4–32°C
• Humidity10–90%
• Protection from excessive heat (e.g., radiators) and accidental spills
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3-33-3
3-33-3
3-3
Installation
Power Supply Requirements
he P C-100 requires 100–240VAC, 50–60Hz, 850W, and a grounded out-
let. he machine can use current in the specified range without adjust-
ment, so there is no voltage-setting switch.
Air Supply Requirements
Air is taken in from vents at the rear and sides and on the bottom of the
machine and exhausted from vents at both sides of the machine. If the ma-
chine does not get enough air or gets air that is too warm, it can overheat.
Overheating can cause the machine to display a warning message, “H[eat]
S[ink] Overheating, Check Air Flow,” or even to shut itself off.
o prevent overheating, position the machine at least 10cm from other
thermal cyclers or walls. If the ambient air temperature exceeds 32°C,
use fans or adjust the air conditioning to cool the air that the machine
receives. Keep the air intake vents clean and free of obstructions such as
loose sheets of paper.
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4-14-1
4-14-1
4-1
Operation
4
Turning the PTC-100 On ............................... 4-2
Using the Control Panel ............................... 4-2
Operation Keys ........................................................ 4-2
Status Indicator Lights ............................................... 4-3
Opening and Closing the Lid ........................ 4-3
Selecting the Correct Sample essel ............ 4-3
0.5ml ubes .............................................................. 4-3
0.2ml ubes .............................................................. 4-3
Microplates .............................................................. 4-4
hin-Walled Vs. hick-Walled ubes .......................... 4-5
Sealing Sample essels ............................... 4-5
Sealing with Oil or Wax ........................................... 4-5
Sealing with the Hot Bonnet and Caps or Film.......... 4-6
Adjusting the Hot Bonnet’s Lid Pressure............. 4-7
Loading Sample essels .............................. 4-8
Using the Sponge Rubber Pad with the 96V Block ... 4-9
Using Oil to Improve hermal Contact ...................... 4-9
Using the Slide Griddle ................................ 4-10
Using the Probe .......................................... 4-10
Customizing the Probe Vessel ................................... 4-10
Adding the Oil .......................................................... 4-11
Loading and Connecting the Probe .......................... 4-12
Running a Protocol under Probe Control .................. 4-12
Calibrating the Probe ............................................... 4-12
Appendix 4-A: Tube, Microplate, and Sealing
System Selection Chart ................................ 4-14
Appendix 4-B: Safety Warning Regarding
Use of 35S Nucleotides ................................. 4-15
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