Molecular Devices ImageXpress Confocal HT.ai User manual

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March 2021
ImageXpress Confocal HT.ai
High-Content Imaging System
User Guide

ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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Contents
Safety Information 7
Safety Symbols in This Guide 7
Safety Labels on the Instrument 8
Protective Housing and Safety Interlocks 9
Laser Safety 10
Electrical Safety 12
Moving Parts Safety 13
Lifting Hazard 13
Chemical and Biological Safety 14
Cleaning and Maintenance 14
Water Immersion Option Safety 15
Chapter 1: ImageXpress Confocal HT.ai System 17
ImageXpress Confocal HT.ai System Features 18
MetaXpress Software Features 21
Theory of Operation 22
Chapter 2: Using the ImageXpress Confocal HT.ai System 27
Starting the System 27
Understanding Status Lights 33
Maintaining the Instrument 34
Shutting Down the System 34
Chapter 3: System Installation and Testing 35
Verifying Device Settings in the Meta Imaging Series Administrator Software 36
Verifying Camera Settings in the Meta Imaging Series Administrator Software 41
Verifying and Backing Up Settings in the MetaXpress Software 42
Chapter 4: Preparing for Acquisition 53
Assay Design 53
Plate Selection 55
Sample Preparation 57
Plate Acquisition Settings 58
Instrument Maintenance 61
Chapter 5: Configuring Plate Acquisition Options 63
Accessing the Plate Acquisition Setup Dialog 64
Plate Acquisition Setup Dialog Layout 65

ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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Plate Acquisition Setup Dialog: Configure Tab, Objective and Camera Tab 69
Plate Acquisition Setup Dialog: Configure Tab, Plate Tab 72
Plate Acquisition Setup Dialog: Configure Tab, Sites to Visit Tab 77
Plate Acquisition Setup Dialog: Configure Tab, Acquisition Tab 90
Specifying the Number of Acquisition Wavelengths 95
Configuring Acquisition Wavelengths 96
Configuring Series Acquisition Options 106
Configuring Journals to Run During Acquisition 115
Configuring Post-Acquisition Analysis Options 118
Plate Acquisition Setup Dialog: Configure Tab, Display Tab 121
Viewing the Summary Panel 123
Saving Plate Acquisition Protocols 124
Running Plate Acquisition Protocols 125
Chapter 6: Customizing the MetaXpress Software 127
Users and Groups in the Meta Imaging Series Administrator Software 128
Custom Toolbars and Taskbars 131
Default Paths for Data 134
Chapter 7: Expansion Solution Options 135
Environmental Control Option 135
Transmitted Light Option 162
Water Immersion Option 179
Chapter 8: Maintenance 189
Maintenance Precautions 189
Routine Maintenance 190
Cleaning the Instrument 190
Light Source Maintenance 192
Replacing the Confocal Disk Box 192
Creating Hardware Profiles 198
Filter Maintenance 200
Objective Maintenance 209
Updating Legacy Shading Correction Settings 233
Turning Off the Status Indicator Lights 236
Obtaining Support 237

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Appendix A: Instrument Specifications 241
Appendix B: Compatible Objectives 243
Appendix C: Filter Specifications and Calibration Compatibility 247
Appendix D: Optional Parts and Accessories 249
Appendix E: File Privileges for the MetaXpress Software 251
Appendix F: Robotic Plate Handling 253
Verifying External Control Settings 254
Appendix G: Electromagnetic Compatibility 255