Motic AE2000 BIN User manual

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We are constantly endeavouring to improve our instruments and to adapt them to the requirements of
modern research techniques and testing methods. This involves modification to the mechanical
structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction manual, including all specifications are
subject to change without notice.

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Content
I. Nomenclature 4
1. Application 4
2. Nomenclature 4
3. Technical data 7
II. Setting-up the Instrument 8
1. Working Environment 8
2. Input voltage and power 8
III. Assembling the Microscope 10
1. Installing the bulb 10
1.1 Halogen bulb 12
1.2 LED module 12
2. Filter Holder 12
3. Mounting the Condenser 13
4. Installing the Objectives 13
5. Mechanical Stage 14
6. Inserting the Eyepieces 15
IV. Microscope Handling 16
1. Interpupillary Distance Adjustment 16
2. Diopter Adjustment 16
3. Coarse and fine focusing 17
4. Coarse focus torque adjustment 17
5. Changing between Halogen and LED illumination 18
6. Brightfield Microscopy 19
7. Phase Contrast Microscopy 19
8. Filter selection 22
9. Auto/Off/On switch function 22
V. Photomicrographic Procedure (Only on AE2000 TRI) 23

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VI. Troubleshooting Table 24
1. Optical and Operating Problems 24
2. Electrical 24
VII. Care and Maintenance 25
1. Lenses and Filters 25
2. Cleaning of Painted or Plastic Components 25
3. When Not in Use 25
4. Warning Label 26

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I. Nomenclature
1. Application
The new Inverted microscope AE2000 is representing the ideal instrument for living cells
observation and other microbiological samples. Due to our Colour Corrected Infinity Optics (CCIS)
the AE2000 offers superior image quality combined with excellent mechanical performance. Daily
work is facilitated by a number of ergonomic features.
2. Nomenclature
Model AE2000

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Model AE2000

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Model AE2000 TRI
Model AE2000 Ergo

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3. Technical data
Technical data AE2000 BIN AE2000 TRI AE2000 Ergo
Optical system Color Corrected Infinity Optics (CCIS)
Total Magnification 40X - 400X
Eyepiece tubes
360° Swiveling Siedentopf with upper and lower position: upper position
offers approx. 40 mm extra viewing height
Interpupillary distance: 48 mm to 75 mm
Binocular 45°Inclined Trinocular 45°Inclined Ergo Inclined: 45°± 15°
Eyepiece N-WF 10X(ø20), wide field, high eye point
Nosepiece Quadruple, inclined, sidewards
Stage Fixed, Dimensions (width x depth): 200 x 239mm
Stage height 192mm
Object guide
Verniers with numerical and alphabetic scale
X direction: numerical scale, readable from right to left
Y direction: alphabetic scale
Objectives
Plan-Achromat 4x, N.A. 0.1, W.D. 12.6mm
Plan-Achromat 10x, N.A. 0.25, W.D. 16.8mm
LWD Plan-Achromat 20x, N.A. 0.3, W.D. 4.7mm
LWD Plan-Achromat 40x, N.A. 0.5, W.D. 3mm
Plan-Achromat PH 4x Ph0, N.A. 0.1, W.D. 12.6mm
Plan-Achromat PH 10x Ph1, N.A. 0.25, W.D. 4.1mm
LWD Plan-Achromat PH 20x Ph2, N.A. 0.3, W.D. 4.7mm
LWD Plan-Achromat PH 40x Ph2, N.A. 0.5, W.D. 3mm
Condenser
N.A. 0.3, W.D. 72mm
N.A. 0.4, W.D. 53mm
N.A. 0.5, W.D. 28mm (in prep.)
Coarse focus 42mm/rot.
Fine focus 0.2mm/rot.
Transmitted
illumination
6V/30W Halogen
3W LED
Dimension: W x D x H 217.5 x 556 x 497mm

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II. Setting-up the Instrument
1. Working Environment
The location should be free from dust, moisture, chemical vapours and from mechanical vibrations.
Don't locate the instrument in bright or direct ambient light, in front of a lamp, or a will-lit bright wall. Best
image will be achieved without significant ambient light.
Environmental specification:
yIndoor use
yAltitude: Max 2000m
yAmbient temperature: 15°C~ 40°C;
yMaximum relative humidity: 75% for temperature up to 31°C decreasing linearly to 50% relative
humidity at 40°C
ySupply voltage fluctuations: Not to exceed ±10% of the normal voltage
yPollution degree: 2 ( in according with IEC60664)
yInstallation/Overvoltage category: 2 (in according with IEC60664)
yAir Pressure of 75kPa to 106 kPa
2. Input voltage and power
Automatic voltage selection works with electrical outlets worldwide. It is advised to always use a power
cord that is rated for the voltage used in your area and that has been approved to meet local safety
standards. Using the wrong power cord could cause fire or equipment damage.
In order to prevent electric fluctuation to the instrument electrics, always turn the power switch on the
instrument off before connecting the power cord.
This equipment must be used with UL60950-1 Listed power supply (E246759), MFR: LI TONG
ELECTRONICS CO., LTD. Model: LTE50E.S2.3.
yInput Voltage: 12VDC
yInput Power: 48W
yPower adaptor input rating: 100-240Vac, 47-63Hz, 1A

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Attention: The plug of the power adaptor is the "disconnect device" for whole unit. To save energy we
recommend to unplug the instrument when not in use.
This device complies with Part 15 of the FCC Rules.
Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and (2) this device must accept any interference
received, including interference that may cause undesired operation.