Motic AE2000 BIN User manual

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We are constantly endeavouring to improve our instruments and to adapt them to the requirements of
modern research techniques and testing methods. This involves modification to the mechanical
structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction manual, including all specifications are
subject to change without notice.

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Content
I. Nomenclature 4
1. Application 4
2. Nomenclature 4
3. Technical data 7
II. Setting-up the Instrument 8
1. Working Environment 8
2. Input voltage and power 8
III. Assembling the Microscope 10
1. Installing the bulb 10
1.1 Halogen bulb 12
1.2 LED module 12
2. Filter Holder 12
3. Mounting the Condenser 13
4. Installing the Objectives 13
5. Mechanical Stage 14
6. Inserting the Eyepieces 15
IV. Microscope Handling 16
1. Interpupillary Distance Adjustment 16
2. Diopter Adjustment 16
3. Coarse and fine focusing 17
4. Coarse focus torque adjustment 17
5. Changing between Halogen and LED illumination 18
6. Brightfield Microscopy 19
7. Phase Contrast Microscopy 19
8. Filter selection 22
9. Auto/Off/On switch function 22
V. Photomicrographic Procedure (Only on AE2000 TRI) 23

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VI. Troubleshooting Table 24
1. Optical and Operating Problems 24
2. Electrical 24
VII. Care and Maintenance 25
1. Lenses and Filters 25
2. Cleaning of Painted or Plastic Components 25
3. When Not in Use 25
4. Warning Label 26

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I. Nomenclature
1. Application
The new Inverted microscope AE2000 is representing the ideal instrument for living cells
observation and other microbiological samples. Due to our Colour Corrected Infinity Optics (CCIS)
the AE2000 offers superior image quality combined with excellent mechanical performance. Daily
work is facilitated by a number of ergonomic features.
2. Nomenclature
Model AE2000

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Model AE2000

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Model AE2000 TRI
Model AE2000 Ergo

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3. Technical data
Technical data AE2000 BIN AE2000 TRI AE2000 Ergo
Optical system Color Corrected Infinity Optics (CCIS)
Total Magnification 40X - 400X
Eyepiece tubes
360° Swiveling Siedentopf with upper and lower position: upper position
offers approx. 40 mm extra viewing height
Interpupillary distance: 48 mm to 75 mm
Binocular 45°Inclined Trinocular 45°Inclined Ergo Inclined: 45°± 15°
Eyepiece N-WF 10X(ø20), wide field, high eye point
Nosepiece Quadruple, inclined, sidewards
Stage Fixed, Dimensions (width x depth): 200 x 239mm
Stage height 192mm
Object guide
Verniers with numerical and alphabetic scale
X direction: numerical scale, readable from right to left
Y direction: alphabetic scale
Objectives
Plan-Achromat 4x, N.A. 0.1, W.D. 12.6mm
Plan-Achromat 10x, N.A. 0.25, W.D. 16.8mm
LWD Plan-Achromat 20x, N.A. 0.3, W.D. 4.7mm
LWD Plan-Achromat 40x, N.A. 0.5, W.D. 3mm
Plan-Achromat PH 4x Ph0, N.A. 0.1, W.D. 12.6mm
Plan-Achromat PH 10x Ph1, N.A. 0.25, W.D. 4.1mm
LWD Plan-Achromat PH 20x Ph2, N.A. 0.3, W.D. 4.7mm
LWD Plan-Achromat PH 40x Ph2, N.A. 0.5, W.D. 3mm
Condenser
N.A. 0.3, W.D. 72mm
N.A. 0.4, W.D. 53mm
N.A. 0.5, W.D. 28mm (in prep.)
Coarse focus 42mm/rot.
Fine focus 0.2mm/rot.
Transmitted
illumination
6V/30W Halogen
3W LED
Dimension: W x D x H 217.5 x 556 x 497mm

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II. Setting-up the Instrument
1. Working Environment
The location should be free from dust, moisture, chemical vapours and from mechanical vibrations.
Don't locate the instrument in bright or direct ambient light, in front of a lamp, or a will-lit bright wall. Best
image will be achieved without significant ambient light.
Environmental specification:
yIndoor use
yAltitude: Max 2000m
yAmbient temperature: 15°C~ 40°C;
yMaximum relative humidity: 75% for temperature up to 31°C decreasing linearly to 50% relative
humidity at 40°C
ySupply voltage fluctuations: Not to exceed ±10% of the normal voltage
yPollution degree: 2 ( in according with IEC60664)
yInstallation/Overvoltage category: 2 (in according with IEC60664)
yAir Pressure of 75kPa to 106 kPa
2. Input voltage and power
Automatic voltage selection works with electrical outlets worldwide. It is advised to always use a power
cord that is rated for the voltage used in your area and that has been approved to meet local safety
standards. Using the wrong power cord could cause fire or equipment damage.
In order to prevent electric fluctuation to the instrument electrics, always turn the power switch on the
instrument off before connecting the power cord.
This equipment must be used with UL60950-1 Listed power supply (E246759), MFR: LI TONG
ELECTRONICS CO., LTD. Model: LTE50E.S2.3.
yInput Voltage: 12VDC
yInput Power: 48W
yPower adaptor input rating: 100-240Vac, 47-63Hz, 1A

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Attention: The plug of the power adaptor is the "disconnect device" for whole unit. To save energy we
recommend to unplug the instrument when not in use.
This device complies with Part 15 of the FCC Rules.
Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and (2) this device must accept any interference
received, including interference that may cause undesired operation.

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III. Assembling the Microscope
1. Installing the bulb
yIn order to prevent electric shock always turn the power switch off (Fig.1) and unplug the power
cord (Fig.2) before replacing the bulb.
yTo remove lamphouse cover, press down lightly (1) and rotate counter clockwise (2). (Fig.3)
Power Switch (Fig.1) Power Cord (Fig.2)
Lamp house Cover (Fig.3)

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yFirmly insert the bulb into the socket pinholes (Fig.4) until it reaches the limit, be careful not to tilt
the lamp when mounting. (Fig.5)
(Fig.4) (Fig.5)
(Halogen Bulb)
yWhen installing the halogen bulb, be careful not to touch the glass surface of the bulb with bare
fingers to avoid fingerprints, grease, etc. Surface pollution can burn the bulb and reduce the
illumination provided by bulb. If surface is contaminated, wipe it clean using lens tissue or
soft cotton.
(Fig.6) (Fig.7)
(LED module)
yFirmly insert the LED module into the socket pinholes until it reaches the limit. This is a Motic patent
design to exchange LED module and halogen bulb on the same socket directly.
yAfter the LED module installation (Fig.6), secure it with the clamp screw by 1.5mm hexagonal
screwdriver supplied with the microscope. (Fig.7)
yReturn lamphouse cover to original position and rotate clockwise to lock into place. The white paint
marked on the cover should face the user. (Fig.8)

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(Fig.8)
1.1 Halogen bulb
The quartz halogen bulb, used as a light source, has higher luminance and colour temperature than
conventional tungsten lamps. The luminance of halogen bulb is approximately four times brighter than the
conventional tungsten lamps.
As long as the lamp voltage is kept constant, the halogen lamp maintains the same level of brightness
and colour temperature regardless of whether it is new or nearing the end of its life span.
1.2 LED module
The LED module is specially designed to be inserted into halogen bulb socket directly converting
halogen illumination to LED illumination. LED is more economical and environmental friendly and
combines the advantages of low heat and long life span.
2. Filter Holder
(Fig.9) (Fig.10)
yFilter holder is located under the lamphouse (Fig.9) for easy replacement of the filter. (Fig.10)

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3. Mounting the Condenser
yMount the condenser on the dovetail mount of the condenser holder (Fig.11) with the aperture
diaphragm lever facing the front. Secure it with the clamp screw by the 2.5mm hexagonal
screwdriver (supplied with the microscope; Fig.12)
(Fig.11) (Fig.12)
yIf Phase contrast is to be used, insert the Phase slider into the slot of the condenser.
The centering screws of Phase slider have to face the front side. (Fig.13)
(Fig.13)
4. Installing the Objectives
yRemove the stage insert from the stage. (Fig.14)
yScrew in the objectives in the nosepiece thread holes in a manner that the magnification increases
with clockwise rotation of the revolving nosepiece. (Fig.15)
yPlace back the stage insert.

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(Fig.14) (Fig.15)
5. Mechanical Stage
yAn attachable mechanical stage is available as an option. This stage allows the precise X/Y
movement of common vessels for cell culture. For choosing the respective vessel carrier, please
contact your local Motic supplier.
ySecure the mechanical stage to the AE2000 plain stage using the two mounting screws (Fig.16)
located beneath the stage on the right side (user facing the front of the instrument).
(Fig.16)

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6. Inserting the Eyepieces
yRemove the dust caps from the eyepiece tubes.
yUse the same magnification eyepieces for both the eyes.
yInsert each eyepiece into the eyepiece sleeve by a twisting movement. (Fig.17)
yShould the rubber eye guards are to be used,fit them in the groove around the eyepiece.
(Fig.17)

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IV. Microscope Handling
1. Interpupillary Distance Adjustment
yBefore adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.
yAdjust the interpupillary distance so that both the right and left field of view become one. This
adjustment will enable the user to observe the specimen with both eyes.
2. Diopter Adjustment
yDiopter adjustment compensates for the differences in vision between the left and right eyes. In
addition to making observation through both eyes easier, this adjustment also reduces the extent to
which focusing is lost when the objective magnification is changed. In particular, this occurs when a
low magnification objective is used.
ySet the diopter on both eyepieces to the “0” position.
ySwing in the 10x objective, focus with one eye only (as everybody has got a “strong” eye, use this
one for this first focussing).
(Fig.18) (Fig.19)
yWhen in focus, close this eye and use the other one. Focus by only using the diopter ring on the
respective eyepiece, do not use the coarse/fine knob!
yChange to a higher magnification and repeat the complete procedure
yAs the focus depth is less in high magnification lenses, a precise adjustment of the diopters here is
easier. Keep this final diopter position for all lenses.

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3．Coarse and fine focusing
yFor focusing of the instrument please use the coarse and fine focus knobs on the left and right side
of the microscope stand.
yThe direction of vertical movement of the revolving nosepiece corresponds to the turning direction
of the focus knobs.
yOne full rotation of the fine focus knob moves the nosepiece 0.2mm in z-direction. One scale unit
on the fine focus knob equals 2 microns.
Please avoid following action:
yNever attempt either of the following actions, since doing so will damage the focusing mechanism.
yRotate the left or right knob while holding the other.
yTurning the coarse and fine focus knobs further than their limit.
4. Coarse focus torque adjustment
yTo increase the torque, turn the torque adjustment ring located behind the left-hand coarse focus in
the direction of the arrow. To reduce the torque, turn the ring in the direction opposite.
(Fig.20)
1. Coarse Focus Torque Adjustment Ring 2. Coarse Focus 3. Fine Focus

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5. Changing between Halogen and LED illumination
yAttention:
Unplug the plug-in power unit from the microscope to avoid any risk of electric shock. Before
replacing the bulb/changing to LED please wait for a sufficient cool-down time of the bulb.
yPress down lightly the lamp house cover and rotate it counter clockwise for removal. (Fig.21)
(Fig.21)
yRemove the bulb from the socket pinholes.
Halogen bulb (Fig.22) LED (Fig.23)
yInstalling the new bulb into the socket pinholes, until it reaches the limited, be careful not to tilt it
when mounting.
yIf installing the halogen bulb, do not touch the glass surface of the lamp with bare fingers. Doing so
will cause fingerprints, grease, etc., to burn onto the lamp surface, reducing the illumination
provided by the bulb. If surface is contaminated, wipe it clean using lens or soft cotton.
yReturn lamphouse cover to original position and rotate clockwise to lock into place. The white paint
marked on the cover should face the user.
yThe halogen bulb holder can be used also for the LED module without modification.

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6. Brightfield Microscopy
ySet the Phase slider to the centre position (in standard configuration this position does not contain
an illumination annulus) (Fig 24). In case all 3 positions of the slider are occupied due to personal
setup of the microscope, pull out the complete slider.
yBring the specimen into focus.
yThe aperture diaphragm of the condenser is needed for adjusting the numerical aperture (N.A.) of
the illumination.
yClosing the aperture diaphragm will lower the resolution and brightness but increase the contrast
and depth of focus. An image with appropriate contrast in most cases can be obtained with an
aperture diaphragm closed down to 2/3.
(Fig.24)
7. Phase Contrast Microscopy
yPhase contrast objectives are labelled “Ph”: Ph0; Ph1; Ph2.
yFor using phase contrast, be sure to use the annular ring in the slider has the same phase label as
the objective:
Ph0: Objective 4x
Ph1: Objective 10x/20x/40x
Ph2: Objectives 20x/40x
yAlways open the aperture diaphragm completely. If the aperture diaphragm is closed, it will obstruct
the annular diaphragm and the phase contrast effect cannot be obtained.
yBring the 10x (Ph1) objective into optical path.
yPosition the Phase annular diaphragm slider to Ph1.