Motic AE2000 Ergo User manual

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We are constantly endeavouring to improve our instruments and to adapt them to the requirements of
modern research techniques and testing methods. This involves modification to the mechanical
structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction manual, including all specifications are
subject to change without notice.

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Content
I. Nomenclature 4
1. Application 4
2. Nomenclature 4
3. Technical data 7
II. Setting-up the Instrument 8
1. Working Environment 8
2. Input voltage and power 8
III. Assembling the Microscope Input Voltage 10
1. Installing the bulb 10
1.1 Halogen bulb 12
1.2 LED module 12
2. Filter Holder 13
3. Mounting the Condenser 13
4. Installing the Objectives 14
5. Mechanical Stage 14
6. Mounting the Eyepieces 15
IV. Microscopic Procedure 16
1. Interpupillary Distance Adjustment 16
2. Diopter Adjustment 16
3. Coarse and fine focusing 17
4. Coarse focus torque adjustment 17
5. Change the illumination between Halogen and LED 18
6. Brightfield Microscopy 19
7. Phase-Contrast Microscopy 19
8. Filter selection 21
9. Auto power off function 21
V. Photomicrographic Procedure (Model AE2000 trinocular) 23
1. Brightfield photomicrography 23

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VI. Troubleshooting Table 24
1. Optical and Operating Problems 24
2. Electrical 25
VII. Care and Maintenance 25
1. Lenses and Filters 25
2. Cleaning of Painted or Plastic Components 25
3. When Not in Use 26
4. Warning lable 26

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I. Nomenclature
1. Application
AE2000 incorporates the Color Corrected Infinity Optical System (CCIS) that offers superior image
quality for the transmitted and reflected illumination in the application of Living organism and tissue
observation, Petri dish observation, Live cell in culture observation.
2. Nomenclature
Model AE2000

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Model AE2000

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Model AE2000 TRI
Model AE2000 Ergo

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3. Technical data
Technical data AE2000 AE2000 TRI AE2000 Ergo
Optical system Color-corrected infinity optics
Total Magnification 40X - 400X
Eyepiece tubes
360° rotatable Siedentopf with upper and lower position: upper position
offers approx. 40 mm extra viewing height
Interpupillary distance: 48 mm to 75 mm
Binocular 45° incline Trinocular 45°incline Ergo incline: 45°± 15°
Eyepiece N-WF 10X(ø20), wide field, high eye point
Nosepiece 4x, inclined backwards
Stage Fixed, Dimensions (width x depth): 200 x 239mm
Stage height 192mm
Object guide Verniers with numerical and alphabetic scale X direction: numerical scale, readable from right to left
Y direction: alphabetic scale
Objectives
Plan-Achromat 4x, N.A. 0.1, W.D. 12.6mm
Plan-Achromat 10x, N.A. 0.25, W.D. 16.8mm
LWD Plan-Achromat 20x, N.A. 0.3, W.D. 4.7mm
LWD Plan-Achromat 40x, N.A. 0.5, W.D. 3mm
Plan-Achromat PH 4x Ph0, N.A. 0.1, W.D. 12.6mm
Plan-Achromat PH 10x Ph1, N.A. 0.25, W.D. 4.1mm
LWD Plan-Achromat PH 20x Ph2, N.A. 0.3, W.D. 4.7mm
LWD Plan-Achromat PH 40x Ph2, N.A. 0.5, W.D. 3mm
Condenser N.A. 0.3, W.D. 72mm N.A. 0.4, W.D. 53mm
N.A. 0.5, W.D. 28mm
Coarse drive 42mm/rev.
Fine drive 0.2mm/rev.
Transmitted
illumination 6V/30W Halogen
3W LED
Dimension: W x D x H 217.5 x 556 x 497mm

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II. Setting-up the Instrument
1. Working Environment
The location should be free from dust, moisture, chemical vapours and from mechanical vibrations.
Don't locate the instrument in bright or direct ambient light, in front of a lamp, or a will-lit bright wall. Best
image will be achieved without significant ambient light.
Environmental specification:
yIndoor use
yAltitude: Max 2000m
yAmbient temperature: 15°C~ 40°C;
yMaximum relative humidity: 75% for temperature up to 31°C decreasing linearly to 50% relative
humidity at 40°C
ySupply voltage fluctuations: Not to exceed ±10% of the normal voltage
yPollution degree: 2 ( in according with IEC60664)
yInstallation/Overvoltage category: 2 (in according with IEC60664)
yAir Pressure of 75kPa to 106 kPa
2. Input voltage and power
Automatic voltage selection works with electrical outlets worldwide. It is advised to always use a power
cord that is rated for the voltage used in your area and that has been approved to meet local safety
standards. Using the wrong power cord could cause fire or equipment damage.
In order to prevent electric fluctuation to the instrument electrics, always turn the power switch on the
instrument off before connecting the power cord.
This equipment must be used with UL60950-1 Listed power supply (E246759), MFR: LI TONG
ELECTRONICS CO., LTD. Model: LTE50E.S2.3.
yInput Voltage: 12VDC
yInput Power: 48W
yPower adaptor input rating: 100-240Vac, 47-63Hz, 1A

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Attention: The plug of power adaptor is the "disconnect device" for whole unit. To save energy and
environmental priciple, we recommend you to pull out the plug if you will not use the device in long time.
This device complies with Part 15 of the FCC Rules.
Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and (2) this device must accept any interference
received, including interference that may cause undesired operation.

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III. Assembling the Microscope
1. Installing the bulb
yIn order to prevent electric shock always turn the power switch off (Fig.1) and unplug the power
cord (Fig.2) before replacing the bulb.
yTo remove lamphouse cover, press down lightly (1) and rotate counter clockwise (2). (Fig.3)
Power Switch (Fig.1) Power Cord (Fig.2)
Lamp house Cover (Fig.3)

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Firmly insert the bulb into the socket pinholes (Fig.4) until it reaches the limit, be careful not to tilt the
lamp when mounting. (Fig.5)
(Fig.4) (Fig.5)
(Halogen Bulb)
yWhen installing the halogen bulb, be care not to touch the glass surface of the bulb with bare
fingers to avoid fingerprints, grease, etc. Surface pollution can burn the bulb and reduce the
illumination provided by bulb. If surface is contaminated, wipe it clean using lens tissue or soft cotton.
(Fig.6) (Fig.7)
(LED module)
yFirmly insert the LED module into the socket pinholes until it reaches the limit. This is Motic patent
design to exchange LED module and halogen bulb on the same socket directly.
yAfter the LED module installation (Fig.6), secure it with the clamp screw by 1.5mm hexagonal
screwdriver supplied with the microscope. (Fig.7)
yReturn lamphouse cover to original position and rotate clockwise to lock into place. The white paint
marked on the cover should face the user. (Fig.8)

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y
(Fig.8)
1.1 Halogen bulb
The quartz halogen bulb, used as a light source, has higher luminance and colour temperature than
conventional tungsten lamps. The luminance of halogen bulb is approximately four times brighter than the
conventional tungsten lamps.
As long as the lamp voltage is kept constant, the halogen lamp maintains the same level of brightness
and colour temperature regardless of whether it is new or nearing the end of its life span.
1.2 LED module
The LED module is specially designed to be inserted into halogen bulb socket directly converting
halogen illumination to LED illumination. LED is more economical and environmental friendly and no
heat issue with a long life span.

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2. Filter Holder
(Fig.9) (Fig.10)
yFilter holder is located under the lamphouse (Fig.9) for easy replacement of the filter. (Fig.10)
3. Mounting the Condenser
yMount the condenser on the dovetail mount of the condenser holder (Fig.11) with the aperture
diaphragm lever and index marks facing the front and secure it with the clamp screw by 2.5mm
hexagonal screwdriver supplied with the microscope. (Fig.12)
(Fig.11) (Fig.12)

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yIf Phase contrast method will be used, insert the Ph annular diaphragm slider with centering
hexagonal socket head screws facing the front. (Fig.13)
(Fig.13)
4. Installing the Objectives
yRemove the stage insert from the stage. (Fig.14)
yInstall the objectives into the nosepiece so that the magnification increases with clockwise rotation
of the revolving nosepiece. (Fig.15)
yPlace back the stage insert.
(Fig.14) (Fig.15)
5. Mechanical Stage
yWhen using a 96-well plate or other petri dish,the mechanical stage combined with suited holder should
be adopted. 3 kinds of Petri dish holder, 2 kinds of Heamacytometer holder and 96 well plates holder are
available for your opinion.
ySecure the mechanical stage to the AE2000 plain stage using the two mounting screws (Fig.16)
located beneath the stage on the right side (user facing the front of the instrument).

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(Fig.16)
yThe specimen can be moved to the desired position by turning the X-axis knob and Y-axis knob.
6. Mounting the Eyepieces
yRemove the dust caps from the eyepiece tubes.
yUse the same magnification eyepieces for both
the eyes.
yInsert each eyepiece into the eyepiece sleeve,
and tighten the clamp screws. (Fig.17)
yShould the rubber eye guards are to be used,
fit them in the groove around the eyepiece.
1. Clamp screws (Fig.17)

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IV. Microscopic Procedure
1. Interpupillary Distance Adjustment
yBefore adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.
yAdjust the interpupillary distance so that both the right and left field of view become one. This
adjustment will enable the user to observe the specimen with both eyes.
2. Diopter Adjustment
yDiopter adjustment compensates for the differences in vision between the left and right eyes. In
addition to making observation through both eyes easier, this adjustment also reduces the extent to
which focusing is lost when the objective magnification is changed. In particular, this occurs when a
low magnification objective is used.
yBefore adjusting the diopter, bring a specimen into focus using the 10x objective.
yTurn the diopter compensation ring on each eyepiece until the adjustment ring is adjusted to the“0”
position.
y
(Fig.18) (Fig.19)
yPosition the 40x objective into the optical path and bring the specimen image into focus by turning
the coarse and fine focus knobs.
yPosition either the 4x or 10x objective into the optical path. Without adjusting the fine and coarse
focus knobs, turn the diopter rings on the eyepieces so that the specimen images in the left and
right eyepieces are focused individually.
yRepeat the above step twice.

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3．Coarse and fine focusing
yF
ocusing function is realized with the coarse and fine focus knobs at the left and right of the
microscope stand.
yThe direction of vertical movement of the stage corresponds to the turning direction of the focus
knobs.
yOne rotation of the fine focus knob moves the stage 0.2mm up or down movement. The graduation
on the fine focus knob is 2 microns.
Please avoid following action:
yNever attempt either of the following actions, since doing so will damage the focusing mechanism.
yRotate the left or right knob while holding the other.
yTurning the coarse and fine focus knobs further than their limit.
4. Coarse focus torque adjustment
yTo increase the torque, turn the torque adjustment ring located behind the left-hand coarse focus in
the direction of the arrow. To reduce the torque, turn the ring in the direction opposite.
1. Coarse Focus Torque Adjustment Ring 2. Coarse Focus Knob 3. Fine Focus Knob
(Fig.20)

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5. Change the illumination between Halogen and LED
yAttention:
Unplug the plug-in power unit from the power outlet to protect user from electric shock and allow for
a sufficient cool-down time of the6V 30W halogen lamp before you replace it.
yPress down lightly and rotate counter clockwise and remove lamphouse cover. (Fig.21)
(Fig.21)
yRemove the bulb from the socket pinholes.
Halogen bulb (Fig.22) LED (Fig.23)
yInstalling the new bulb into the socket pinholes, until it reaches the limited, be careful not to tilt it
when mounting.
yIf installing the halogen bulb, do not touch the glass surface of the lamp with bare fingers. Doing so
will cause fingerprints, grease, etc., to burn onto the lamp surface, reducing the illumination
provided by the bulb. If surface is contaminated, wipe it clean using lens or soft cotton.
yReturn lamphouse cover to original position and rotate clockwise to lock into place. The white paint
marked on the cover should face the user.
yThe same halogen bulb holder can be used for both LED illumination module.

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6. Brightfield Microscopy
ySet the Phase annular diaphragm slider in the centre position without phase annular diaphragm.
(Fig.24)
yBring the specimen image into focus.
yThe condenser aperture diaphragm is provided for adjusting the numerical aperture (N.A.) of the
illuminating system of the microscope. It is important
because it determines the resolution of the image,
contrast, depth of focus and brightness.
yStopping down the aperture diaphragm will lower the
resolution and brightness but increase the contrast
and depth of focus. By stopping down the N.A. of the
condenser to 2/3 of the N.A. of the objective, a good
image of suitable contrast will be obtained.
(Fig.24)
7. Phase-Contrast Microscopy
yPhase contrast objectives are labelled “Ph”: Ph0; Ph1; Ph2.
yFor phase contrast microscopy, be sure to use the annular diaphragm that has the same phase
label as the objective, despite of the magnification of the objective.
yMove the aperture diaphragm lever on the condenser to fully open position.
Always fully open the aperture diaphragm for phase contrast microscopy. If aperture diaphragm is
closed, it will obstruct the annular diaphragm and the phase contrast effect cannot be obtained.
yBring the 10x (Ph1) objective into optical path.
yPosition the Phase annular diaphragm slider to Ph1.
yPosition the Phase annular diaphragm slider to Ph0 when using 4x objective.
And position the Phase annular diaphragm slider to Ph2 when using 20x (Ph2) or 40x (Ph2)
objective.
yRemove either eyepiece from the eyepiece tube and insert the phase centering telescope instead.
(Fig.25)
yRotate the eyepiece of the centering telescope to focus on both the phase plate image of the
objective and the annular diaphragm image of the phase slider.