NanoEnTek ADAM-MC User manual

USER MANUAL
NESMU-AMC-001E (V.6.1)
ADAM-MC
A New Standard of
Automated Cell Counter
Explore the Cellular Universe!

2
All the materials in this user guide are protected by Korean and international copyright
laws. They cannot be reproduced, translated, published or distributed without the
permission of the copyright owner.
ADAM-MC, User’s Manual
Website : www.nanoentek.com
E-mail : [email protected]
Manufactured by
NanoEntek Inc.
851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel: [phone]
Fax: [phone]
NanoEntek America, Inc.
220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA
Tel: [phone]
Fax: [phone]
The information in this manual is described as correctly as possible and is applicable
to the latest firmware and software versions, but it may be changed without prior
consent or notification.
Copyright 2008, by NanoEntek, Inc.
All rights reserved. Published in Korea.
Documentation : NESMU-AMC-001E (V.6.1)
Revision History :
V.0.0 October 2008
V.1.5 February 2009
V.2.0 June 2009
V.3.0 September 2009
V.3.5 January 2010
V.4.0 November 2010
V.5.0 February 2012
V.5.1 March 2014
V.6.0 June 2020
V.6.1 July 2021

ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. 3
Table of contents
Product contents 4
Safety information 5-7
Product specifications 8
Description of ADAM-MC 9-10
Introduction 11-12
Overview 11
Basic principals of counting 12
Getting started 13-19
Environmental requirement 13
Installation 14
Start-up Screen 15
Error message during booting 16
Menu setting 17
System information 17
Chip selection 18
Counting method 19
General operation 20-25
Introduction 20
Preparing cell 20
Counting cell 20
Operating ADAM-MC 21
Icon function 22
Result analysis 23
Error code 24
Maintenance and cleaning 25
Software installation 26-33
Introduction 26
Getting started 26
Installation 27-28
Function guide 29
Function buttons 30
Data list 31
Information 31
Graph 32
Sample image of error message 33
Trouble shooting 34
Warranty 35
Product list 36

4
Product Contents
ADAM-MC
AccuChip kit
Upon
receiving the
instrument
The contents of the ADAM-MC are listed below:
The contents of the ADAM AccuChip Kit are listed below :
Item
Main device
User manual
USB Cable
Installation CD
Key Pad
Power Cord
Fuse
External video monitor (Optional)
Quantity
1
1
1
1
1
1
2
1
Item
Disposable Chip
Solution T
Solution N
Available test Q'ty
Accuchip2x Kit
(Cat. No:AD2K-200)
200pcs (2 channel)
2 x 12.5ml
1 x 12.5ml
Min.200 test/kit
Max.400 test/kit
( Only total cell count )
Accuchip4x Kit
(Cat. No: AD4K-200)
200pcs (4 channel)
2 x 12.5ml
1 x 12.5ml
Min.400 test/kit
Max.800 test/kit
( Only total cell count)
AccuStain solution
(Cat. No:ADR-1000)
N/A
4 x 12.5ml
2 x 12.5ml
• Examine the instrument carefully for any damage incurred during transit.
• Ensure that all parts of the instrument including accessories listed above
are included with the product.
• Any damage claims must be filed with the carrier.
• The warranty does not cover in-transit damage.
• See the 13 page to install the instrument.
• Upon receipt, store AccuStain solution at room temperature.

ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. 5
Safety information
Safety
precautions
1. Always ensure that the power supply input voltage matches
the voltage available in your location.
2. For operation environment, See page 12.
3. This machine is air-cooled so its surfaces become hot during
operation. When installing it, leave a space of more than 10 cm
(4 inches) around it.
4. Never insert metallic objects into the air vents of the instrument
as this could result in electrical shock, personal injury and
equipment damage.
5. Always set the main switch on the power supply unit to off before
connecting the power cord to the wall outlet.
6. Always ensure that the grounding terminal of the instrument
and that of the wall outlet are properly connected. The power
cord should be connected to a grounded, 3-conductor power
outlet.
7. To avoid potential shock hazard, make sure that the power
cord is properly grounded.
8. Do not position the equipment in a position that is difficult to
disconnect the equipment.
9. Be sure to set the main switch to off, unplug the power cord
and lock the stage before moving.
10. If the instrument is broken or dropped, disconnect the cord
and contact an authorized service person. Do not disassemble
the instrument.
11. Use only authorized accessories.
12. Use this equipment only as specified in this manual and as
specified in any documentation associated with its components.
Any use of the equipment in an unspecified manner is strongly
discouraged and may result in damage or injury as cautioned
by signed warnings.

6
Safety information
Safety
symbols
The symbols used in the ADAM-MC and the manual are
explained below:
The Caution symbol denotes a risk of safety
hazard.
ON (Power)
Protective earth (Ground)
The CE mark symbolizes that the product conforms
to all applicable European Community provisions
for which this marking is required. Operation of the
ADAM automated cell counter is subject to the
conditions described in this manual.
The protection provided by the device may be
impaired if the instrument is used in a manner
not specified by the manufacturer.
Caution, Biohazard
Protective measures must be used in dealing
with biologically hazardous materials such as
carcinogenic reagents.
Disposal of your old appliance
1. When this crossed-out wheeled bin symbol
is attached to a product, it means the product
is covered by the European Directive 2012/19/EU.
2. All electrical and electronic products should
be disposed of separately from the municipal
waste stream via designated collection facilities
appointed by the government or the local
authorities.
3. The correct disposal of your old appliance
will help prevent potential negative consequences
for the environment and human health.
4. For more detailed information about disposal
of your old appliance, please contact your city
office, waste disposal service or visit our
web-site,
Meaning
Symbol
www.nanoentek.com.

ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. 7
Battery inside device
· Risk of explosion if battery is replaced by an incorrect type.
· This battery is not replaceable by a user.
· Refer to a qualified personnel.
Cover
· Do not remove a cover or dissemble a case.
· There is no adjustable components inside the instrument.
· If malfunction is found, refer to a service personnel.
Manual
· Do not attempt to service the equipment unless this manual has been
consulted and is understood.
· This manual is available in English only.
· Failure to heed this warning may result in injury to service provider, operator
from electric shock, mechanical or other hazards.
Sample handling
· Wear gloves during sampling. User's sample may have the infectious
biohazardous substance.
Waste
· After using AccuChips, appropriately dispose it asbiohazardous waste.
· Do not reuse the AccuChips.
Operator
· Must have the general knowledge of cell counting procedure and bio
safety to handle the sample that may have the infectious biohazardous
substance.
Safety information
Warnings

8
Product specifications
ADAM-MC
AccuChip
Accessories
AccuStain
Solution T,N
AccuChip 2x AccuChip 4x Measuring range
Analysis time
Loading sample
vol. per test
Measuring sample
vol. per test
Power cord
Fuse
Keypad
PI (Propidium Iodide) staining of total cells (T)
and non-viable cells (N).
5 × 104to 4×106cells/mL
45 sec.~ 2min./test
20 μL/test (AccuChip2x )
13 μL/test (AccuChip4X )
8.5 μL/test (AccuChip2X )
3 μL/test (AccuChip4X )
1.5 m
250 VAC, 3 A;
F3.15AL250V
USB Type / 1.3 m
Voltage
Current
Fuse
Objective lens
LED
CCD camera
Filter
Weight
Size (W × L × H)
Degree of protection
100-240V~, 50/60 Hz
max. 1.8A, max 100W
F3.15AL250V
4 X
Green LED
B/W CCD
Excitation filter
Dichroic filter
Emission filter
9 kg
220 × 375 × 250 mm
IPX0

ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. 9
Description of ADAM-MC
Front view of
ADAM-MC
The ADAM-MC, an automated cellcounter, is a benchtop automated
cell counter that performs cell count and viability measurements
using AccuStain solution.
The front view showing various parts of the ADAM-MC is shown
below:
Key Pad
Control Button
Door
LCD
Control buttons
Eject : Ejects the chip holder from the ADAM-MC.
Start : Performs all procedures of automatic counting
Lock : Protects the alignment of stage from external shock when
the ADAM-MC is moved to the other places.
It is strongly recommended to lock ADAM-MC before turning it off.
Door : Chip holder comes out here.
LCD : Displays the process and the result.
keypad : Inputs the sample number less than 3 characters and
press 'Enter' button.

10
Description of ADAM-MC
Front view of
ADAM-MC
The rear view showing various parts of the ADAM-MC.
Power Plug
Power Switch
Fan
Key Pad Port
RS232C Port
USB Port
Video Port
Fan : Cooling fan
Power switch : Main power on/off switch
Power plug : ADAM-MC power cord connection plug
USB port : Connect to computer with USB Cable
RS232C port : Not connect (Port for only QC and Service)
Key pad port : Keypad connection port
Video Port : External video monitor port

ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. 11
Introduction
Overview Until now,cell counting and viability measurement for many types
of cells have been performed manually using hemocytometer
with TrypanBlue exclusion method, which is to distinguish
viable cells from non-viable cells. One drawback of this
method, however, is the propensity for the staining of
artifacts; another drawback is that the naked eye can only
differentiate between cells in a limited concentration range in
the hemocytometerchamber. This combined with the potential
problem of cell aggregation and limited sample volume leads
to the common variation of counts normally associated with this
method.
To address these problems, NanoEntek has developed the
ADAM-MC, which is based on a fluorescent microscopy
technique for counting cells. The ADAM-MC utilizes sensitive
fluorescence dye staining, LED optics and CCD detection
technologies to make the cell analysis more accurate and reliable.
To count cells using ADAM-MC, the cells are mixed with a
Propidium Iodide (PI) stain and directly pipettedon to a disposable
plastic chip. The chip is then loaded onto a precision stage.
An ADAM-MC system is automatically focused onto the chip
and cells that have been stained are recorded by a sensitive
CCD camera. The image results are automatically processed
generating the cell count which is displayed on the front of the
instrument.
Simple. Fast. Accurate. Reliable.

12
Introduction
Basic
principals of
counting
ADAM-MC is based on staining mammalian cell DNA with a
fluorescent dye, Propidium Iodide (PI). PI does not enter cells
with intact cell membranes or active metabolism. In contrast,
cells with damaged membranes or with inactive metabolism are
unable to prevent PI entering the cell. As a result, the nuclei of
non-viable cells will only be stained. The ADAM-MC provides
two kinds of staining solutions. AccuStain Solution T for the
total cell counting is composed of the fluorescent dye (PI)
and lysis solution. AccuStain solution N for the non-viable cell
counting is composed of the fluorescent dye and PBS.
In order to measure the total concentration of cells, the plasma
membranes of all the cells must be disrupted to stain all
the Nuclei with PI. The process of disrupting and staining is
achieved by treatment with AccuStain Solution T.
In the second solution, live cells remain intact and are not
stained.
Only the non-viable cells are stained and detected. After
treatment, the prepared cells will be loaded into the chip. The
viability will be automatically calculated in the ADAM-MC
software after each measurement of the total cells and the non-
viable cells.
• A: Total cell
• B: Non-viable cell

ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. 13
Getting started
Environmental
requirements
To obtain the best results, install the ADAM-MC in a location
following conditions:
1. Room temperature between 20 and 35 °C.
· Not recommended for cold room use (≤ 4 °C).
· At low temperature (≤10 °C), warm up the ADAM-MC for 10 min.
2. Not exposed to direct sun light.
3. Not subject to direct or continuous vibration.
4. Not subject to intense magnetic or electromagnetic fields.
5. Relative humidity between 0–95 %.
6. Area free from corrosive gases or other corrosive substances.
7. Area with very little dust or other airborne particles.
8. Allow a 10cm minimum space around the instrument for
proper air flow.
9. Not allow to put heavy material on top of ADAM-MC.

14
Getting started
1. Remove all components of ADAM-MC from their box.
2. Place the instrument in a flat level and dry surface.
3. Plug to power cord into the electrical outlet.
· Be sure to use only the power cord supplied with your instrument.
· Powering the instrument with an unapproved power cord may
damaged the instrument.
4. Plug to Keypad.
5. Turn on the power switch.
· Make sure that the main power switch is In the “ I ” (ON) position.
Installation

ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. 15
Getting started
Start-up
screen
1. System booting.
2. System will go through
self diagnostic tests.
· If you get an error message,
please contact your local
distributor or
[email protected]
3. The home screens will be
displayed as the image,
no errors are detected.

16
Getting started
Error
message
during
booting
1. It appears when turning
off without locking device.
2. Push the lock button before
turn off the device. If this
message still appears after
restart, contact your local
distributor or
[email protected]
1. It appears when booting
not working properly.
2. Turn off main power and
restart device.
3. If this message still appears
after restart, contact your
local distributor or
[email protected]
1. It appears when a chip is
inserted during start up.
2. Remove the chip from a
device, and do not turn on
the device with a chip.
3. If this message continues
to display when no chip
is inserted, contact your
local distributor or
[email protected]

ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. 17
Getting started
Menu setting
System
information
1. You can set the menu as
you press the ‘*’ button
on the keypad from the
screen for inputting sample
numbers.
2. You can select the number
from the menu.
1. Check the device versions
and date which have been
installed in the device.
2. Select number 4 key from
the menu, and press the
‘Enter’ key.
3. The screen will return to
the menu screen auto-
matically.
[System Info.]
1. Version : MC V1.21
2. Date : Sep 23, 11
CORE1 : 1.87
CORE2 : 1.52
CORE3 : 1.53

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Chip
selection
4. After pressing the ‘Enter’
key, the screen will return
to the menu screen auto-
matically.
1. Press ‘1’ for chip selection.
· You can select the number
from the menu.
2. User can select the 2 kinds
of chip type.
· ‘1’ : Two channel chip
(AccuChip2X )
· ‘2’ : Four channel chip
(AccuChip4X ).
3. Press ‘1’ or ‘2’, and Enter
key.
Getting started

ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. 19
Counting
method
3. Press ‘1’ or ‘2’ and ‘Enter’
key.
4. After pressing the ‘Enter’
key, the screen will return
to the menu screen auto-
matically.
1. Press ‘2’ for counting
method.You can change
the capture frame mode.
2. You can select the 60 or
30 frames in 2CH mode
and 22 or 11 frames in
4CH mode for counting
as well.
• 2CH
High Precision : 60 frames capture
High Speed : 30 frames capture
• 4CH
High Precision : 22 frames capture
High Speed : 11 frames capture
Getting started

20
1. Cultivate the required number of cells.
2. Add an appropriate volume of growth media or PBS to dilute
to a final concentration of 5x104cells/mL to 4x106cells/mL.
☞
Note : Concentration out of this range will result in errors.
Refer to page 23 for more information about errors.
3. Thoroughly mix the cell pellet by vortexing.
4. Check visually if any cell clumps or agglomerates are remained.
General operation
Introduction
Preparing
cell
Counting cell
Instruction is provided in this section for preparing the cell
sample with AccuStain solution for use with disposable
AccuChip for automated cell count using the ADAM-MC.
• AccuChip 2x
• AccuChip 4x
Total Cell
1. Add 50 μL of your sample to 50 μL supplied
AccuStain solution T.
2. Mix gently by pipetting up and down.
3. Load 20 μL sample mixture to the AccuChipon
T channel.
Total Cell
1. Add 50μL of your sample to 50μL supplied
AccuStain solution T.
2. Mix gently by pipetting up and down.
3. Load 13μL sample mixture to the AccuChip
on T1 or T2 channel.
Non-viable Cell
1. Add 50 μL of your sample to 50 μL supplied
AccuStain solution N.
2. Mix gently by pipetting up and down.
3. Load 20 μL sample mixture to the AccuChipon
N channel.
Non-viable Cell
1. Add 50μL of your sample to 50μL supplied
AccuStain solution N.
2. Mix gently by pipetting up and down.
3. Load 13μL sample mixture to the AccuChip
on N1 or N2 channel.
Non-Viable
Non-viable Non-viable
Total Total
Total