NanoEnTek ADAM CellT User manual

Instruction Manual
A New Standard of Automated Cell Counter
Copyright NanoEntek, Inc. All rights reserved.
ADAM

All the materials in this instruction manual are protected by Korean and international copyright laws.
They cannot be reproduced, translated, published or distributed without the permission of the copyright
owner.
ADAM-CellT Instruction Manual
Website : www.nanoentek.com
E-mail : [email protected]
Manufactured by
NanoEntek, Inc.
851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel. [phone]
Fax. [phone]
NanoEntek America, Inc.
220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA
Tel: [phone]
Fax: [phone]
The information in this manual is described as accurately as possible.
Firmware and software changes and updates may change without prior consent or notification.
Copyright © 2019 by NanoEntek Inc.
All rights reserved. Published in Korea.
Documentation: NESMU-ACT-001E
Revision history: V.0.0 OCT 2019
V.0.1 DEC 2021
V.0.5 AUG 2022
V.0.6 SEP 2022

Table of contents
Introduction
General description
Technology
Basic principle of counting
Setting
Setting
Wifi
Remote support
Update
Auto logout
Backup
Recovery
Image
Save
Mail
Approval
User
User
User manage
Privilege
Password option
Log manage
Document manage
Deleted list
Product Contents
ADAM-CellT
AccuChip kit
Upon receiving the instrument
Maintenance and cleaning
Trouble shooting
Warranty
Technical specifications
Product list
Safety precautions
Safety symbols
Warnings
Technical Support
General Operation
Introduction
Sample preparation
Counting cell
Measure
Run sample
Result analysis
Result analysis -Error code
Data
QC mode
QC slide edit
QC slide result edit
QC slide result
Data list
Edit
Power off
Lock
Power off
Getting Started
Environmental requirements
Power on and initial display
Error messages during booting
Count setting
Product Description
Front view of ADAM-CellT
Rear view of ADAM-CellT
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7
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56

6
Introduction
General
Description
The ADAM-CellT is a benchtop automated cell counter designed to
perform cell counting and viability measurements using AccuStain Solution.

ADAM-CellT, A New Standard of Automated Cell Counter 7
Introduction
Technology Until now, cell counting and viability measurement for many types of cells
have been performed manually using hemocytometer with Trypan Blue
exclusion method, which is to distinguish viable cells from non-viable cells.
One drawback of this method, however, is the propensity for the staining
of artifacts; another drawback is that the naked eye can only differenti-
ate between cells in a limited concentration range in the hemocytometer
chamber. This combined with the potential problem of cell aggregation
and limited sample volume leads to the common variation of counts nor-
mally associated with this method.
To address these problems, NanoEntek has developed the ADAM-CellT,
which is based on a fluorescent microscopy technique for counting cells.
The ADAM-CellT utilizes sensitive fluorescence dye staining, LED optics and
CMOS detection technologies to make the cell analysis more accurate and
reliable.
To count cells using ADAM-CellT, the cells are mixed with Propidium Iodide
(PI) stain/ Acridine Orange (AO) and directly pipetted on to a disposable
plastic chip. The chip is then loaded onto a precision stage. An ADAM-CellT
system is automatically focused onto the chip and cells that have been
stained are recorded by a sensitive CMOS camera. The image results are
automatically processed generating the cell count which is displayed on
the front of the instrument. Simple. Fast. Accurate. Reliable.
AccuChip
CMOS Camera
Emission Filter
Dichroic Filter
Objective Lens
Excitation filter
Lenses
Green LED Mirror

8
Introduction
Basic principle
of counting
ADAM-CellT is an instrument which counts mammalian cell DNA by staining
with a fluorescent dye, Propidium Iodide (PI) or Acridine Orange (AO).
PI does not enter cells with intact membranes or active metabolism.
In contrast, cells with damaged membranes or cells with inactive metabolism
are unable to prevent PI entering the cell. As a result, the nuclei of cell
membrane-damaged normal cells or non-viable cells will be stained.
Solution TPI is composed of the PI and cell membrane lysis buffer.
Since lysis buffer in Solution TPI changes intact cell membrane to damaged cell
membrane condition, both viable cells and non-viable cells can be stained. AO
included in Solution TAO is permeable dye which permeates cell membrane
and stains DNA. Regardless of the condition of the cell membrane or active
metabolism, AO can stain both viable cells and non- viable cells.
The ADAM-CellT provides two kinds of staining solutions: AccuStain Solution
T for the total cell counting and AccuStain Solution N for the non-viable cell
counting. AccuStain Solution T is categorized into SolutionTPI and Solution TAO.
AccuStain Solution N for the non-viable cell counting is composed of the PI
alone. After staining samples, the prepared cells will be loaded into the chip.
The viability will be automatically calculated in the ADAM-CellT software after
each measurement of the total cells and the non-viable cells.
T Channel
A: Total cell / B: Non-viable cell
N Channel
AccuChip 4 Channel
AccuChip 2 Channel
*Viability(%)= X 100
A
(A-B)
T1 N1 T2 N2
.

ADAM-CellT, A New Standard of Automated Cell Counter 9
Introduction
ADAM-CellT
AccuChip kit
Upon receiving
the instrument
The contents of the ADAM-CellT are listed below:
The contents of the ADAM-CellT’s AccuChip Kit are listed below:
• Examine the instrument carefully for any damage incurred during transit.
•Ensure that all parts of the instrument including accessories listed above
are included with the product.
• Any damage claims must be filed with the carrier.
• The warranty does not cover in-transit damage.
• Upon receipt, store AccuChip at room temperature. AccuStain Solution
should be stored at 2~8°C
Item Quantity
Main device 1
Power cord 1
USB hub 1
Adapter 1
User’s manual 1
Support for 21 CFR Part 11 Compliance 1
AccuChip Kit 1
USB Wifi dongle 1
External Hard disk 1
Calibration Bead 1
Labeling 1
Inspection Sheet 1
Printer (optional) 1
QC slide (optional) 1
Item AccuChip2x Kit
(Cat. No: AD2K-200)
AccuChip4x Kit
(Cat. No: AD4K-200)
AccuStain Solution
(Cat. No: ADR-1000)
Disposable
Chip 200pcs (2 channel) 200pcs (4 channel) N/A
Solution T 12.5 mL x 2ea 12.5 mL x 2ea 12.5 mL x 4ea
Solution N 12.5 mL x 1ea 12.5 mL x 1ea 12.5 mL x 2ea
Available
test Q’ty
Min. 200 test/kit Min. 400 test/kit
Max. 400 test/kit
(Only total cell count)
Max. 800 test/kit
(Only total cell count)
Product Contents

10
Introduction
Front view of
ADAM-CellT
The front view of ADAM-CellT is shown below:
Control buttons Description
① Door Slide holder is inserted and ejected.
② Power Power on / off.
③ LCD Display processes and results.
④ START Performs all procedures of automatic counting.
⑤ LOCK
Protects the alignment of stage from external shock when
ADAM-CellT is being moved.
Lock ADAM-CellT before turning it off or moving it.
⑥ EJECT Ejects the slide holder from ADAM-CellT. Functions as unload.
Product Description
Door
Power
LCD
⑥
④
⑤
①
②
③

ADAM-CellT, A New Standard of Automated Cell Counter 11
Introduction
Rear view of
ADAM-CellT
The rear view of ADAM-CellT is shown below:
Port Description
①USB Port Port for software update and save the data.
②PC port Connects with PC
③Power Plug Connects ADAM-CellT power cord to wall outlet
Product Description
①USB Port ②PC Port ③Power plug
Do not use the PC port. This port does not recognize USB.
!CAUTION
②

12
Introduction
Power on and
Initial Display
Getting Started
Environmental
requirements
To ensure correct operation and stable performance,
install the ADAM-CellT in a location which meets the following conditions:
1. Use at room temperature between 20 and 35 °C
• Not recommended for cold room use (≤ 4 °C).
2. Do not expose the device to direct sunlight.
3. Do not subject the device to direct or continuous vibration.
4. Do not subject the device to intense magnetic or electromagnetic fields.
5. Do not install the device in high-humidity environment.
6. Location of device should be free from corrosive gases or other corrosive
substances.
7. Ensure minimal contact with dust or other airborne particles.
8. Allow a 10 cm (4 inches) minimum space around the device for proper
airflow.
9. Do not place any objects on the device.
1. Check the connection of ADAM-CellT and power cord.
2. Press the power button for 2~3 seconds.
If you get an error message,
please contact your local distributor or [email protected].
If booting is successful and no errors are detected,
the home screens will be displayed as below.
At low temperature (≤10 °C), allow the device to warm up for 10 minutes at
ambient temperature before use.
!CAUTION
•Do not tilt the device too much in the forward when connecting the power cord.
•Do not move the device after connecting power cord.
- When you connect the power cord to ADAM-CellT even without power on the device,
it will go through self diagnostic tests.
!CAUTION

ADAM-CellT, A New Standard of Automated Cell Counter 13
Introduction
Error Messages
during booting
[System State]
Error code Cause
0x00000C00 Failure of X-axis sensor
0x00007000 Failure of Y-axis sensor
0x00008000 Failure of Z-axis sensor
0x06000000 Failure of Locking module sensor
Getting Started
It appears when booting is not working properly.
Turn off main power and restart device.
If this message still appears after restarting,
contact your local distributor or [email protected].

14
IntroductionGetting Started
Count setting Set the conditions in the ‘Setting’tap before counting.
[AccuChip]
Set the AccuChip according to you are using.
[Cell size]
Set the minimum and maximum size of cell.
[Dilution factor]
When diluting sample, set the Dilution factor.
Factor values for the AccuStain Solution is already applied.
!CAUTION
2Ch4Ch
Accuchip
4Ch 2Ch
Accuchip
Cell size Min
5
Max 8
0
Dilution factor
T1 N1 T2 N2
1.0
[Solution type]
Set the AccuStain Solution type (Propidium iodide (PI) or Acridine orange (AO))
TPI/N TAO/N

ADAM-CellT, A New Standard of Automated Cell Counter 15
IntroductionGeneral Operation
Introduction Instruction is provided in this section for preparing the sample with
AccuStain Solution for use with disposable AccuChip for automated cell
count using the ADAM-CellT.
Please check the procedure of sample preparation and testing below.
For more detailed information, please refer to the next page.
2. Load the mixed sample. Then, wait 1 minute for the sample settling.
1. Mix the sample with AccuStain Solution.
3. Insert AccuChip. Get the result.
AccuStain Solution
50 μL
Sample
50 μL
OR
- Result -
2 channel: 23μL
4 channel: 13μL
T channel: Total cell
N channel: Non-viable cell

16
Introduction
T1 N1 T2 N2
When you load of the sample mixture to the AccuChip, please be careful
not to make bubbles.
!NOTE
Concentration out of this range will result in errors.
Refer to page 21 for more information about errors.
!NOTE
Counting cell
General Operation
Sample
preparation
3. Thoroughly mix the cell pellet by vortexing.
4. Check visually if any cell clumps or agglomerates are remained.
[AccuChip 2x]
[AccuChip 4x]
Total Cell Non-viable Cell
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution T.
2) Voltex the tube vigorously.
3) Load 23 µL sample mixture to the
AccuChip on Tchannel. Then, wait
1 minute for the sample settling.
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution N.
2) Voltex the tube vigorously.
3) Load 23 µL sample mixture to the
AccuChip on Nchannel. Then, wait
1 minute for the sample settling.
Total Cell Non-viable Cell
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution T.
2) Voltex the tube vigorously.
3) Load 13 µL sample mixture to the
AccuChip on T1 or T2 channel.
Then, wait 1 minute for the sample
settling.
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution N.
2) Voltex the tube vigorously.
3) Load 13 µL sample mixture to the
AccuChip on N1 or N2 channel.
Then, wait 1 minute for the sample
settling.
Total
Total
T/N1 T/N2
Non-viable
Non-viable
1. Cultivate the required number of cells.
2. Add an appropriate volume of growth media or PBS to dilute to a final
concentration of 5 x 104cells/mL to 4 x 106cells/mL (TPI/N solution).
When using TAO/N solution, prepare to a final concentration of 5 x 104
cells/mL to 2 x 107cells/mL.

ADAM-CellT, A New Standard of Automated Cell Counter 17
Introduction
T1 N1 T2 N2
T1 N1 T2 N2
T1 N1 T2 N2
General Operation
Counting cell
Correct volume
Low volume
[Sample loading error]
Be cautious of loading the correct volume of the sample into AccuChip.
The instrument will not detect low or high sample volumes.
!WARNING
Avoid bubbles which may negatively aect the result.
!CAUTION

18
IntroductionGeneral Operation
Counting cell
[AccuChip insert error]
Completely insert AccuChip face up, in the direction of the arrow on the
slide. The instrument will not detect if slides are inserted incorrectly.
See pictures below for proper insertion.
!WARNING
• Please insert or remove the AccuChip when the slide holder is fully ejected.
• When the test is finished, please remove the AccuChip from the slide holder.
!CAUTION
(O )
(X )
(X )
(X )
Correctly inserted
inserted upside down
Not fully inserted
Inserted in opposite direction

ADAM-CellT, A New Standard of Automated Cell Counter 19
IntroductionMeasure
Run Sample Start counting process by pressing‘START’.
It may take about 2 minutes longer for auto focus at the initial test.
While the test is in progress, you can check the cell images of each channel.

20
Introduction
Measure
Result Analysis The result will be displayed after being automatically calculated by ADAM-
CellT software.
Title Number of
Total cell
Number of
Non-Viable cell Viability
Viability 01 T1 (1.10E6) N1 (5.50E5) 50%
Viability 02 T2 (2.20E6) N2 (5.50E5) 75%
•The viability will be automatically calculated by the ADAM-CellT software after
each measurement of the total cells and the non-viable cells.
• First, the total cell number and second, non-viable cell number were measured
and then the cell viability is calculated as subtracting non-viable cell counting
numbers from total cell counting.
* 1.10E6 = 1.10 X 10 cells/mL
6
‘PRINT’ button will be automatically activated when portable printer
(optional) is connected.
!NOTE