Next Advance Bullet Blender 50 User manual

The Bullet Blender® 50
Models: BB50-AU, BB50-DX
Congratulations!
Congratulations on your purchase
of a Bullet Blender® 50 by Next
Advance, Inc., for lysing, disrupting,
and homogenizing your samples.
which explains proper operation of the
instrument. This manual is posted on
our website, www.nextadvance.com.
Click the SUPPORT button on the left-
hand menu bar and then on the
appropriate link to the manual.
Blender will become an essential tool in
your laboratory and we wish you
success with your work.
Contents
Parts of the Bullet Blender. ................. 2
Setup. .................................................. 3
Operation. ........................................... 3
Protocols and Sample Settings. ........... 4
Cleaning. ............................................. 5
Troubleshooting. ................................. 5
Support. ............................................... 5
Specifications ...................................... 5
Warranty. ............................................ 6
Warnings and Cautions ....................... 6
Disclaimer. .......................................... 7
Contact Info. ....................................... 7
DOMINIQUE DUTSCHER SAS

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® 50
Parts of the Bullet Blender® 50
SYMBOLS USED ON THE BULLET BLENDER 50
Start Operation
This product complies with European Low Voltage and EMC Directives
Please dispose the test tubes and the BBX or BBY Bullet Blender in
accordance with local regulations
DOMINIQUE DUTSCHER SAS

SETUP
Place the Bullet Blender® 50 on a
stable, level lab bench. Carry it by
grasping the bottom sides. Plug the AC
power supply cord connector into the
AC Input Receptacle on the right side
of the Bullet Blender® and then insert
the plug into a wall outlet. You should
see lights on the time setting flash for
several seconds, indicating that the unit
is receiving power. It is now set up.
OPERATION
To use your Bullet Blender 50, lift
open the instrument cover, twist the
handle of the sample plate cover
counter clock-wise until the white
arrow is in the unlocked position (see
figure below) and lift the sample plate
cover out of the instrument.
The figure above shows labels that
indicate if the sample plate cover is in
the locked or unlocked position. Here, it
is shown in the locked position.
Insert the 50 mL sample tubes in the
protective sleeves and place the sleeves
into the sample tube plate. The threads
on the sample tubes must be dry and
the caps must be screwed on very
tightly. Use a minimum of 2 sleeves, so
that all the energy from the Bullet
Blender is not concentrated on a single
tube. For best results, the sleeves should
be evenly spaced. Replace the cover
and rotate it to lock it in place. Fold
down the handle on the cover, and then
close the lid.
Set the time and the speed to the
button. At each three minute interval an
LED will light up, indicating its
progress. The air cooling will continue
for 30 seconds after the motor times
out.
will stop the instrument.
Example:
The figure above shows the BB50-AU
model Bullet Blender set to run for 12
minutes at a speed of 8 after running for
6 minutes. To operate, press the
LED light on the number 1 will light
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up. After 2 minutes the second LED
light by the number 2 will light up. And
so on. At the setting shown above, after
12 minutes, the Bullet Blender 50 will
stop. The BB50-
light that comes on when the internal
compartment of the instrument is
refrigerated. On the BB50-DX model,
ambient air is circulating through the
inside of the instrument during
operation and for 30 seconds after the
cycle is complete.
The BB50-AU unit contains a dry
ice cooling compartment. To use, fill
the dry ice cooling compartment with at
least 2 lbs of dry ice. Always use
cryogenic gloves when handling dry
ice. Do not use the inner portion of the
dry ice cooling compartment to
transport the ice. Do not fill the dry ice
cooling compartment with water ice.
Make sure that the dry ice cooling
compartment lid is properly sealed. To
pre-cool the instrument, after filling the
compartment with dry ice, run the
instrument for 5 minutes.
PROTOCOLS AND SAMPLE
SETTINGS
The following ratio should be used
as a guideline for determining the
amount of beads and buffer to use given
a certain sample size - 1 volume/mass
of tissue: 1 volume of beads: 2 volumes
of buffer. For more specific information
regarding the use of various beads as
well as specific protocol information,
please refer to our website:
www.nextadvance.com.
For 50 mL tubes, do not operate
with more than 3.5g of sample, or a
total of 20mL of buffer, tissue, and
beads (per tube).
Cutting the tissue into smaller
pieces will generally yield better
results. Tissue with a high aspect ratio
(long, thin strips) will homogenize
better than tissue that is round or cubic.
Do not operate the Bullet Blender
50 using the same tubes for more than
30 minutes.
Protocols for many types of
samples are posted on our website, at
www.protocols.nextadvance.com
Notes:
Use Axygen® brand or Corning®
brand polypropylene, plug seal cap,
skirted (self-standing) 50 mL tubes. If
there is a different brand of 50 mL tube
you would like to use, please contact us
at [e-mail]. There
may be some discoloration of the
sleeves and of the tubes after
homogenization. This is normal and a
by product of the workings of the
Bullet Blender 50, which is designed to
impact the tubes with as much energy
as possible.
CLEANING
If you wish to clean your Bullet
Blender, com. clean the outside of the
unit only with mild detergent, water and
a soft cloth. Under normal conditions,
the Bullet Blender 50 should never need
to be disassembled for cleaning. In the
case of a large spill, please contact Next
Advance at www.nextadvance.com. Do
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not touch or tamper with the
electronics.
TROUBLESHOOTING
In addition to the tips given below,
a thorough list of troubleshooting tips is
at bbFAQ.nextadvance.com
start, make sure the Power Switch is in
the ON (I) position. Make sure that the
position. The plug of the power supply
cord might not be in a live wall outlet
or the power supply connector might
not be fully inserted in the AC Input
Receptacle of the Bullet Blender 50.
If the unit stops working, turn the
system off for 15 minutes to allow the
electronics to reset. If the Bullet
Blender 50 does not turn on after this
period, contact customer service.
If the caps on the 50 mL tubes
loosen, make sure that the screw
threads between the lids and the caps
are dry when you close the caps or
screw them on, so that there is enough
friction for the caps to remain tight.
Using recommended types of tubes will
minimize cap failure.
For best results, the tubes and
sleeves should be evenly spaced when
placed in the Bullet Blender.
If the 4°C light is not coming on,
frost from condensation may be
blocking the air passage. Remove the
inner dry ice compartment and gently
agitate it or replace the ice. If mist
coming out of the dry ice cooling
container seems excessive, make sure
that the lid of the dry ice container is
closed securely.
SUPPORT
FAQs, protocols, and other helpful
information are available on our
website, www.nextadvance.com. Click
on the Bullet Blender, then on the
appropriate link. If you cannot find an
answer there, please contact customer
service by email at
[e-mail] or by
telephone at [phone] or (800)
738-1681.
SPECIFICATIONS
Size: BB50-DX is 35 cm (14 in.) deep x
38 cm (15 in.) wide x 38 cm (15 in.)
high.
BB5050-AU is 35 cm (14 in.) deep x 46
cm (18 in.) wide x 38 cm (15 in.) high.
Weight: 33lbs (15 kg)
Power Requirement: 100-240 V, 50-60
Hz, 2.5-4.5 A
Capacity: 8 of 50 mL skirted (self-
standing) polypropylene tubes.
Relative Humidity: 5 - 90% non-
condensing
Operating Temperature: 4 - 40°C
Altitude: <2000m
Storage Temperature: -40 to 50°C
Meets
requirements (-CE
models only)
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WARRANTY
Next Advance warrants its Products
against defects in materials and
workmanship for time periods which
vary according to the Product. Within
these time periods, Next Advance will
replace or repair, without charge to the
original purchaser, any part which is
defective.
Bullet Blender Two years
The warranty is void if the Product
is defective due to product accident,
product modification, exposure to
radiation other than for sterilization,
connection to an improper electrical
supply, lack of proper maintenance,
contamination, improper installation or
misuse. If the product is used in a
manner not specified by the
manufacturer, the protection provided
by the equipment may be impaired. The
warranty shall also not apply to defects
arising from fire, flood, lightning or
other conditions unrelated to correct
operation of the Product.
price for the Product (2) repair of the
Product, or (3) replacement of the
Product or defective parts. Evidence of
purchase by the original purchaser is
required. Next Advance may also
request documentation of proper
maintenance, if applicable.
Next Advance makes no other
warranty, express or implied, with
respect to its Products. NEXT
ADVANCE MAKES NO
WARRANTY RESPECTING THE
MERCHANTABILITY OF THE
PRODUCTS OR THEIR
SUITABILITY OR FITNESS FOR
ANY PARTICULAR PURPOSE OR
USE. Next Advance shall not be liable
for, indirect, special, incidental or
consequential damages of any nature.
Any recovery for any claim shall be
limited to the original purchase price
for the product.
Provide proof of purchase and provide
normal care and maintenance.
WARNINGS AND CAUTIONS
operating.
Do not carry the instrument by the dry
ice compartment. Instead, lift from the
bottom sides of the instrument.
Do not operate Bullet Blender 50
instruments with fewer than 2 sleeves in
place.
Do not open lid or cover when the
Bullet Blender is in use.
Wear cryogenic gloves when handling
dry ice.
Do not use the inner container of the
dry ice cooling compartment to
transport ice.
Do not use regular water ice in the dry
ice cooling compartment.
Do not insert fingers or objects other
than recommended tubes into sample
tube holes.
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Use caution when closing Bullet
Blender lid- do not close on fingers.
Use recommended tubes only.
No user serviceable parts are inside.
For indoor use only.
Pollution Degree 2 per EN 61010-1.
Overvoltage Category II per EN 61010-
1.
Enclosure Protection: Not Protected
Against the ingress of Moisture.
Sound Pressure Level: Up to 90dBA.
Use hearing protective devices that
reduce exposure to below 85 dBA
during prolonged exposure.
Do not immerse in liquid.
Before touching the Bullet Blender,
touch a bare metal surface to discharge
static electricity.
DISCLAIMER
Next Advance, Inc. makes no
representations or warranties,
expressed, statutory or implied,
regarding the fitness or merchantability
of this product for any particular
purpose. In no event shall Next
Advance be liable for incidental or
consequential damages. Next Advance,
Inc. is not liable for any damages,
including but not limited to, lost profits,
lost savings, or other incidental or
consequential damages arising from
ownership or use of this product, or for
any delay in the performance of its
obligations under the warranty due to
causes beyond its control.
Next Advance, Inc. also reserves
the right to make any improvements or
modifications to the product described
in this manual at any time, without
notice of these changes. Next Advance,
Inc. products are not designed,
intended, or authorized for use in
applications or as system components
intended to support or sustain human
life, as a clinical medical device for
humans, or for any application in which
the failure of the product could create a
situation where personal injury or death
may occur.
All brand and product names used
in this manual are the trademarks of
their respective owners.
CONTACT INFO
Next Advance, Inc.
Averill Park, NY, USA
Telephone [phone]
www.nextadvance.com
[e-mail]
DOMINIQUE DUTSCHER SAS

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DOMINIQUE DUTSCHER SAS