Beckman Coulter JA-25.50 User manual

J-TB-056F
October 2007
JA-25.50 Fixed Angle Rotor
Used In Beckman Coulter J2 and
Avanti®J Series Centrifuges
© 2007 Beckman Coulter, Inc.
Printed in the U.S.A.

2
JA-25.50 Rotor
SAFETY NOTICE
This safety notice summarizes information basic to the safe use of the rotor described in this
manual. The international symbol displayed above is a reminder to the user that all safety
instructions should be read and understood before operation or maintenance of this equip-
ment is attempted. When you see the symbol on other pages throughout this publication, pay
special attention to the specific safety information presented. Observance of safety precau-
tions will also help to avoid actions that could damage or adversely affect the performance of
the rotor. This rotor was developed, manufactured, and tested for safety and reliability as part
of a Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if
used in a centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centri-
fuge that has been modified without Beckman Coulter’s approval.
Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that such fluids are free of micro-organisms. Some of the most
virulent—Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria, and certain
systemic fungi—further emphasize the need for aerosol protection. Handle other infectious
samples according to good laboratory procedures and methods to prevent spread of disease.
Because spills may generate aerosols, observe proper safety precautions for aerosol contain-
ment. Do not run toxic, pathogenic, or radioactive materials in this rotor without taking
appropriate safety precautions. Biosafe containment should be used when Risk Group II
materials (as identified in the World Health Organization Laboratory Biosafety Manual) are
handled; materials of a higher group require more than one level of protection.
The rotor and accessories are not designed for use with materials capable of developing
flammable or explosive vapors. Do not centrifuge such materials in nor handle or store them
near the centrifuge.
Although rotor components and accessories made by other manufacturers may fit in the
JA-25.50 rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of
other manufacturers’ components or accessories in the JA-25.50 rotor may void the rotor
warranty and should be prohibited by your laboratory safety officer. Only the components
and accessories listed in this publication should be used in this rotor.
Do not run an empty rotor. Place filled tubes in at least two opposing cavities. Make sure that
filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to
the same level with liquid of the same density.
If disassembly reveals evidence of leakage, you should assume that some fluid escaped the
rotor. Apply all appropriate safety and decontamination procedures to the centrifuge and
accessories.
Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section
on RUN SPEEDS.
Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion
begins in scratches and may open fissures in the rotor with continued use.
!
!
!
!
!
!
!
!

3
*Avanti J-25 I and Avanti J-25, 50-Hz models
at 4°C (at 24°C ambient) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 000 rpm (69 700 ×g)
at 7°C (at 24°C ambient) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 000 rpm (75 600 ×g)
NOTE: At ambient temperatures above 24°C, the temperatures listed above may not be
achievable.
†The critical speed range is the range of speeds over which the rotor shifts so as to rotate about
its center of mass. Passing through the critical speed range is characterized by some vibration.
‡Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius
and speed (r
ω
2) to the standard acceleration of gravity (g) according to the following formula:
where ris the radius in millimeters,
ω is the angular velocity in radians per second
(2
π RPM /60), and gis the standard acceleration of gravity (9807 mm/s2). After substitution:
RCF rω2
g
---------=
RCF 1.12rRPM
1000
------------
⎝⎠
⎛⎞
2
=
JA-25.50 Rotor
JA-25.50 FIXED ANGLE ROTOR
SPECIFICATIONS
Maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 000 rpm*
Critical speed range†. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 600 to 800 rpm
Density rating at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Maximum allowable imbalance of opposing loads. . . . . . . . . . . . . 2 grams
Relative Centrifugal Field‡at maximum speed
at rmax (108 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 600 ×g
at rav (73.2 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 200 × g
at rmin (38.5 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 000 ×g
kfactor at maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 418
Conditions requiring speed reduction . . . . . . . . . . . . . . . . .see RUN SPEEDS
Number of tube cavities (round-bottom). . . . . . . . . . . . . . . . . . . . . . . . . . 8
Available bottles and tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 3
Nominal tube dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 ×104 mm
Nominal tube capacity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 mL
Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 400 mL
Approximate acceleration time to maximum speed
(rotor fully loaded)
in an Avanti®J centrifuge (to 25 000 rpm) . . . . . . . . . . . . . . 3 1/2min
in a J2 series centrifuge (to 21 000 rpm) . . . . . . . . . . . . . . . . 1 3/4min
Approximate deceleration time from maximum speed
(rotor fully loaded, max. brake)
in an Avanti®J centrifuge (from 25 000 rpm). . . . . . . . . . . . 1 3/4min
in a J2 series centrifuge (from 21 000 rpm). . . . . . . . . . . . . . 2 1/2min
Rotor weight, empty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.1 kg (17.8 lb)
Rotor weight, maximum sample mass . . . . . . . . . . . . . . . . 8.7 kg (19.1 lb)
Rotor material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . aluminum
Rotor entry code for microprocessor-controlled J2 series
instruments. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25.5
rmin
rav
rmax
34°
Axis of Rotation

4
JA-25.50 Rotor
DESCRIPTION This rotor has been manufactured in a registered ISO 9001 or 13485
facility for use with the specified Beckman Coulter centrifuges.
The JA-25.50 fixed angle rotor, rated for 25 000 rpm, is designed
to hold up to eight 50-mL tubes at a 34-degree angle (see Figure 1).
Used in Beckman Coulter Avanti®J and J2 series centrifuges, the
JA-25.50 rotor develops centrifugal forces that can efficiently pellet
cells from large volumes, or cell particles from tissue homogenates.
Short column methods (i.e., partially filled tubes) may also be used to
purify large quantities of virus in a cushion gradient.
The dual-locking lid assembly allows the rotor to be loaded into and
removed from the centrifuge with the lid attached. The rotor may be
placed under a safety hood before the lid is attached or removed. The
rotor is also available with a single-locking lid assembly. The rotor
has been tested1with both lid configurations to demonstrate contain-
ment of microbiological aerosols under normal conditions of the
associated Beckman Coulter centrifuge when used and maintained
as instructed.
The JA-25.50 rotor has a patented fluid-containment annulus, located
below the O-ring sealing surface (see Figure 2). If tubes are overfilled
or if leakage occurs during centrifugation, the annulus holds enough
volume that all of the liquid is kept inside the rotor—even if all eight
tubes leak at the same time. This feature virtually eliminates the
escape of liquid into the centrifuge chamber. Finger-grip grooves in
the area around and between each tube cavity (see Figure 2) provide
extra room for fingers as tubes are loaded and unloaded.
Figure 1. The JA-25.50 Fixed Angle Rotor (dual-locking lid shown)
1Validation of microbiological containment was done at an independent third-party testing facility (CAMR, Porton Down, UK, or USAMRIID,
Ft. Detrick, MD, U.S.A.). Improper use or maintenance may affect seal integrity and thus containment.
Lid
Daisy Knob
Small O-Ring
(011757)
Large O-Ring
(870612)
Rotor Body
Dual-locking lid shown
Rotor
Labware
Lid

5
JA-25.50 Rotor
The rotor and lid are made of anodized aluminum. Two O-rings made
of Buna N rubber, both located in the rotor lid, help to maintain
atmospheric pressure inside the rotor during centrifugation when they
are properly lubricated. Vertical pins in the rotor drive hole mate with
the centrifuge drive hub to prevent the rotor from slipping during
acceleration and deceleration.
See the Warranty at the back of this manual for warranty information.
Figure 2. Fluid-Containment Annulus and Finger-Grip Grooves
PREPARATION AND USE
Specific information about the JA-25.50 rotor is given here. Use the
Rotors and Tubes for Beckman Coulter J Series Centrifuges manual
(publication JR-IM) and the centrifuge instruction manual along with
this manual for complete rotor and accessory operation.
➠NOTE
Although rotor components and accessories
made by other manufacturers may fit in the
JA-25.50 rotor, their safety in this rotor cannot
be ascertained by Beckman Coulter. Use of
other manufacturers’ components or accessories
in the JA-25.50 rotor may void the rotor
warranty and should be prohibited by your
laboratory safety officer. Only the components
and accessories listed in this publication should
be used in this rotor.
Fluid
Containment
Annulus
O-Ring
Sealing Surface
Finger-Grip
Grooves

6
JA-25.50 Rotor
PRERUN SAFETY CHECKS
Read the Safety Notice page at the front of this manual before using
the rotor.
1. Make sure that the rotor, lid, and all tubes or bottles and accesso-
ries are clean and show no signs of corrosion or cracking.
2. Verify that the bottles or tubes being used are listed in Table 3.
3. Refer to Appendix A in Rotors and Tubes for the chemical
compatibilities of all materials used.
ROTOR PREPARATION
For runs at other than room temperature, refrigerate or warm the
rotor beforehand for fast equilibration.
1. Be sure that metal threads in the rotor are clean and lightly but
evenly lubricated with Spinkote™ lubricant (306812). Also ensure
that O-rings are lightly but evenly coated with silicone vacuum
grease.
2. Load the filled containers symmetrically into the rotor. (Refer
to TUBES AND BOTTLES on page 12 for information about
containers.) If fewer than eight tubes are being run, they must
be arranged symmetrically in the rotor (see Figure 3). Opposing
tubes must be filled to the same level with liquid of the same
density.
Figure 3. Typical Examples of Arranging Tubes in the Rotor.
Two, four, six, or eight tubes can be centrifuged per run,
if they are arranged in the rotor as shown.

7
JA-25.50 Rotor
➠NOTE
Do not run an empty rotor. Place filled tubes in
at least two opposing cavities.
OPERATION
• Precool the rotor in the centrifuge or in a refrigerator before use—
especially before short runs—to ensure that the rotor reaches the
set temperature. A suggested precooling cycle is a minimum of
30 minutes at 2000 rpm at the required temperature.
• If fluid containment is required, use capped tubes or bottles. It is
strongly recommended that all containers carrying physiological
fluids be capped to prevent leakage.
• If you are using an Avanti J series centrifuge, select the JA-25.50
rotor.
• If you are using a microprocessor-controlled J2 series centrifuge,
enter rotor code 25.5. Refer to your centrifuge instruction manual
for additional information.
INSTALLING THE ROTOR
1. To prevent the rotor from sticking to the centrifuge drive hub,
apply a thin coat of Spinkote lubricant to the rotor drive pins and
to the lid knob threads.
2. Lightly coat the large and small lid O-rings with silicone vacuum
grease.
3. Dual-locking lid only. Place filled tubes into the rotor, then place
the lid on the rotor and turn the daisy knob to the right (clockwise)
until secure. Do not overtighten.
4. Carefully lower the rotor straight down onto the centrifuge drive
spindle hub. Do not drop the rotor onto the spindle hub.
5. Slowly turn the rotor around the drive spindle to make sure that it
is properly seated.
Tie-down Knob
Daisy
Knob
Lubricate
Threads
Dual-Locking Lid

8
JA-25.50 Rotor
6. Secure the rotor to the centrifuge drive spindle as follows:
Dual-locking lid: press the lid knob down and turn it to the right
(clockwise) until secure, no more than two full turns. Do not
overtighten.
Single-locking lid: place filled tubes into the rotor, then place the
lid on the rotor. Press the lid knob down and turn it to the right
(clockwise) until secure. Do not overtighten.
WARNING
!If the rotor is left in the centrifuge between
runs, make sure that the rotor is seated on the
drive hub and that the tie-down knob is tight
before each run.
TEMPERATURE
To ensure that the JA-25.50 rotor reaches the required temperature
during the run, follow the appropriate instructions below for the
model of J centrifuge being used.
Avanti J Series Centrifuges
Enter the run temperature according to the instructions in your
centrifuge instruction manual. No additional input is required.
J2 Series Centrifuges
Enter the required run temperature and the appropriate temperature
compensation units (see Tables 1 and 2) on the centrifuge control
panel as follows.
Tie-down Knob
Lubricate
Threads
Single-Locking Lid

9
JA-25.50 Rotor
Table 1. Temperature Compensation Settings for Analog-
and Microprocessor-Controlled Centrifuges
(Models J2-HS, J2-MI, and J2-MC).
Interpolate if intermediate values are required.
An “N” indicates that the rotor cannot achieve the required temperature at this speed.
Table 2. Temperature Compensation Settings for the Model J2-HC Analog
Centrifuge. Interpolate if intermediate values are required.
An “N” indicates that the rotor cannot achieve the required temperature at this speed.
Analog J2 Series Centrifuges (Models J2-HS and J2-HC)
1. Turn the SET knob on the centrifuge control panel to the required
sample temperature.
2. Find the compensation value in Table 1 for Models J2-HS or
Table 2 for Model J2-HC that corresponds to the required temper-
ature and run speed. Set the COMP dial to that setting. (Interpolate
if intermediate values are required.)
➠NOTE
Temperature settings for J-21 and J2-21 series
centrifuges must be empirically determined.
Rotor
Speed
(rpm)
Required Sample Temperature (°C)
–20 –10 2 5 10 20 40
21 000 N N –7 –5 –6 –5 –9
18 000 N –5 –5 –5 –5 –4 –7
15 000 0 –2 –3 –3 –2 –2 –6
10 000 0 0 –1 0 –1 0 –3
Rotor
Speed
(rpm)
Required Sample Temperature (°C)
–20 –10 2 5 10 20 40
18 000 N –8 –6 –6 –6 –6 –5
15000–7–6–5–5–5–4–4
10000–5–3–2–3–4–3–2

10
JA-25.50 Rotor
Microprocessor-Controlled J2 Series Centrifuges
(Models J2-MI and J2-MC)
1. Press the [TEMP] key on the centrifuge control panel and then use
the keypad to enter the sample temperature.
2. Find the compensation value in Table 1 that corresponds with the
set temperature and run speed.
3. Press [COMP ADJ]. The word “COMP” flashes below the
TEMPERATURE display and the display flashes.
4. Use the keypad to enter the required compensation value. Press
the [±.] key to enter a minus sign; pressing it again will remove the
minus sign.
5. Check the temperature display. (If the entry is incorrect, press [ce]
and reenter the digits.)
6. When the entry is correct, press [ENTER/RECALL].
➠NOTE
To clear a COMP ADJ entry, press
[COMP ADJ], [0], and [ENTER/RECALL].
RUN PROCEDURE
When the rotor is properly loaded and installed in the centrifuge, you
are ready to perform the run. Refer to the applicable centrifuge
instruction manual for operating instructions.
WARNING
!Operator error or tube failure may generate
aerosols. Toxic, pathogenic, or other
hazardous materials must not be run in
this rotor unless all appropriate safety
precautions are taken. Also see the Safety
Notice at the beginning of this manual.

11
JA-25.50 Rotor
!CAUTION
Make sure that the rotor is properly seated on
and securely fastened to the drive hub before
each run.
REMOVAL AND SAMPLE RECOVERY
!CAUTION
If disassembly reveals evidence of leakage, you
should assume that some fluid escaped the rotor.
Apply all appropriate safety and decontamina-
tion procedures to the centrifuge and
accessories.
Dual-Locking Lid
1. Place one hand on the rotor to keep it from turning. Grasp the
round lid knob with your other hand and turn the knob to the left
(counterclockwise) until the stem disengages from the drive hub
threads.
2. Hold the knob or place your hands on the rotor and lift the rotor
straight up off the drive hub.
3. Place the rotor on a counter or bench top under a safety hood.
4. Grasp the daisy knob and turn it to the left (counterclockwise)
until it disengages.
5. Remove the lid and set it aside.

12
JA-25.50 Rotor
Single-Locking Lid
1. Unscrew the lid knob.
2. Remove the lid and set it aside.
3. Lift the rotor straight up off the drive hub to avoid bending or
damaging the hub.
TUBES AND BOTTLES
The JA-25.50 rotor uses the tubes and bottles listed in Table 3.
Be sure to use only those items listed, and to observe the maximum
speed limits and fill volumes shown. (Maximum fill volume is the
maximum amount that can be centrifuged in the container listed.)
Refer to Appendix A in Rotors and Tubes for information on the
chemical compatibilities of tube and accessory materials.
Temperature Limits
• Plastic containers have been centrifuge tested for use at
temperatures between 2 and 25°C. For centrifugation at other
temperatures, pretest containers under anticipated run conditions.
• If plastic containers are frozen before use, make sure that they are
thawed to at least 2°C prior to centrifugation.
Thickwall Tubes
Thickwall polyallomer, polypropylene, and polycarbonate tubes can
be run partially filled (at least half filled) with or without caps, but all
opposing tubes for a run must be filled to the same level with liquid of
the same density. Do not overfill capless tubes.

13
JA-25.50 Rotor
Table 3. Available Tubes and Bottles for the JA-25.50 Rotor.
Use only the items listed here and observe fill volumes and maximum run speeds.
a Maximum speeds listed are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter, but because of
manufacturing variances no guarantee of performance or fit is expressed or implied.
bPolypropylene, package of 25.
cAbove 20°C fill polypropylene tubes at least half full. Containers may be filled less than or equal to the maximum fill volumes provided in
this table.
dUltem; package of 2. Ultem is a registered trademark of GE Plastics.
eCommercially available. Observe manufacturer’s speed and fill volume guidelines.
Tube or Bottle Required Accessory
Max Speeda/
RCF/
kFactor
Dimensions
and Volume Description Part Number
Max. Fill
Volume
(mL) Description Part
Number
29 ×104 mm
50 mL polycarbonate bottle
assy w/cap
(pkg/24)
361693 40 none — 25 000 rpm
75 600 ×g
418
29 ×104 mm
50 mL polyallomer bottle assy w/cap
(pkg/24)
361694 40 none — 25 000 rpm
75 600 ×g
418
29 ×104 mm
50 mL polycarbonate bottle, screw-cap
(pkg/25)
357002 40 none — 25 000 rpm
75 600 ×g
418
29 ×104 mm
50 mL polyallomer bottle, screw-cap
(pkg/25)
357003 40 none — 25 000 rpm
75 600 ×g
418
29 ×104 mm
50 mL polycarbonate thickwall tube,
snap-on cap
(pkg/25)
363664 36.5 natural 29-mm cap
red 29-mm cap
green 29-mm cap
yellow 29-mm cap
blue 29-mm cap
356264b
357359b
357360b
357361b
357362b
25 000 rpm
75 600 ×g
418
29 ×104 mm
50 mL polypropylene thickwall tube,c
snap-on cap
(pkg/25)
357005 36.5 natural 29-mm cap
red 29-mm cap
green 29-mm cap
yellow 29-mm cap
blue 29-mm cap
356264b
357359b
357360b
357361b
357362b
25 000 rpm
75 600 ×g
418
29 ×104 mm
50 mL polycarbonate thickwall tube,
no cap (pkg/25)
363647 35 none — 25 000 rpm
75 600 ×g
418
29 ×104 mm
50 mL polypropylene thickwall tube,
no cap (pkg/25)
357007 35 none — 25 000 rpm
75 600 ×g
418
16 ×80 mm
10 mL polycarbonate bottle, screw-cap
(pkg/25)
355672 10 adapter 361703d25 000 rpm
63 240 ×g
350
9.5 ×38 mm
1.5 mL polyallomer tube (pkg/500) 357448 1.3 adapter 361705d25 000 rpm 50 960 ×g
182
9.5 ×38 mm
1.5 mL polypropylene tube (pkg/500) 356090 1.3 adapter 361705d25 000 rpm
50 960 ×g
182
25 ×105 mm glass tubeeee adapter 870331 e

14
JA-25.50 Rotor
Polycarbonate and Polyallomer Bottles
Capped polycarbonate and polyallomer bottles may be centrifuged
completely filled, or partially filled (not less than half full).
Containers placed in opposing positions in the rotor must be filled
to the same level.
RUN TIMES
To pellet particles of known sedimentation coefficient s, calculate the
run time tusing the kfactor (395) of the rotor.2Time is in hours and s
is in Svedberg units.
(1)
For example, at 25 000 rpm, mammalian viruses (s= 700 S) can be
pelleted in
(2)
RUN SPEEDS
The centrifugal force at a given radius in a rotor is a function of run
speed. Comparisons of forces between different rotors are made by
comparing the rotors’ relative centrifugal fields (RCF). When rota-
tional speed is selected so that identical samples are subjected to the
same RCF in two different rotors, the samples are subjected to the
same force (see Table 4).
2Based on sedimentation in water at 20°C. Assumes full tubes.
tk
s
-- 25 000
actual run speed
---------------------------------------
⎝⎠
⎛⎞
2
=
t395
700
---------25 000
25 000
----------------
⎝⎠
⎛⎞
230 minutes.≈=
SPEED
RPM/RCF

15
JA-25.50 Rotor
Table 4. Relative Centrifugal Fields for the JA-25.50 Rotor.
Entries in this table are calculated from the formula
RCF = 1.12 r (RPM/1000)2
and then rounded to three significant digits.
Rotor
Speed
(rpm)
Relative Centrifugal Field (×g)
At rmax
(108 mm) At rav (73.2 mm) At rmin (38.5 mm)
25 000
23 000
21 000
75 600
64 000
53 300
51 200
43 400
36 200
27 000
22 800
19 000
19 000
17 000
15 000
43 700
35 000
27 200
29 600
23 700
18 500
15 600
12 500
9 700
13 000
11 000
9 000
20 400
14 600
9 800
13 900
9 900
6 640
7 300
5 700
3 490
7 000
5 000
3 000
1 000
5 930
3 020
1 090
121
4 020
2 050
737
82
2 110
1 080
388
43
Relative Centrifugal Fields for the JA-25.50 Rotor
Speed (rpm)
RCF (x g)
0
10 000
20 000
30 000
40 000
50 000
0 5 000 10 000 15 000 20 000 25 000
60 000
70 000
80 000
r
max
r
av
r
min

16
JA-25.50 Rotor
Do not select rotational speeds higher than the maximums you
have determined to be appropriate for your protocols (in no case
above 25 000 rpm). When solutions more dense than 1.2 g/mL are
centrifuged in this rotor, use equation (3) to calculate the reduced
maximum allowable rotor speed.
(3)
CARE AND MAINTENANCE
MAINTENANCE
➠NOTE
Do not use sharp tools on the rotor that could
cause scratches in the rotor surface. Corrosion
begins in scratches and may open fissures in the
rotor with continued use.
• Periodically (at least monthly) inspect the rotor, especially inside
cavities, for rough spots or pitting, white powder deposits
(frequently aluminum oxide), or heavy discoloration. If any
of these signs are evident, do not run the rotor. Contact your
Beckman Coulter representative for information about the Field
Rotor Inspection Program and the rotor repair center.
• Regularly lubricate the metal threads in the rotor with a thin, even
coat of Spinkote lubricant. Failure to keep these threads lubricated
can result in damaged threads.
• Regularly apply silicone vacuum grease to the O-rings. Replace
O-rings about twice a year or whenever worn or damaged.
Refer to Appendix A in Rotors and Tubes for chemical compatibili-
ties of rotor and accessory materials. Your Beckman Coulter repre-
sentative provides contact with the Field Rotor Inspection Program
and the rotor repair center.
reduced maximum speed = (25 000) 1.2 g/mL
density of tube contents
---------------------------------------------------------
Lid
Daisy
Knob
Small
O-Ring
(011757)
Large
O-Ring
(870612)
Rotor Body
Check
for
Corrosion

17
JA-25.50 Rotor
CLEANING
Wash the rotor and rotor components immediately if salts or other
corrosive materials are used or if spillage has occurred. Do not allow
corrosive materials to dry on the rotor.
Under normal use, wash the rotor frequently (at least weekly) to
prevent buildup of residues.
1. Remove the O-rings before washing.
2. Wash the rotor and lid in a mild detergent, such as Beckman
Solution 555™, that won’t damage the rotor. The Rotor Cleaning
Kit (339558) contains two special plastic-coated brushes and two
quarts of Solution 555 for use with rotors and accessories. Dilute
the detergent 10 to 1 with water.
➠NOTE
Do not wash rotor components in a dishwasher.
Do not soak in detergent solution for long
periods, such as overnight.
3. Thoroughly rinse the cleaned rotor and components with distilled
water.
4. Air-dry the rotor and lid upside down. Do not use acetone to dry
the rotor.
5. Apply a thin, even coat of silicone vacuum grease to both lid
O-rings before replacing them in the lid.
Clean metal threads every 6 months, or as necessary. Use a brush and
concentrated Solution 555. Rinse and dry thoroughly, then lubricate
lightly but evenly with Spinkote to coat all threads.
Periodically remove the O-rings and wipe clean as necessary. Clean
the O-ring grooves with a cotton-tipped swab. Reapply a light film of
silicone vacuum grease.
Rotor Cleaning
Kit (339558)
Small O-Ring
Large
O-Ring

18
JA-25.50 Rotor
DECONTAMINATION
If the rotor (and/or accessories) becomes contaminated with radioac-
tive material, it should be decontaminated using a solution that will
not damage the anodized surfaces. Beckman Coulter has tested a
number of solutions and found two that do not harm anodized
aluminum: RadCon Surface Spray or IsoClean Solution (for
soaking),3and Radiacwash.4
➠NOTE
IsoClean can cause fading of colored anodized
surfaces. Use it only when necessary and
remove it promptly from surfaces.
While Beckman Coulter has tested these methods and found that they
do not damage components, no guarantee of decontamination is
expressed or implied. Consult your laboratory safety officer regarding
the proper decontamination methods to use.
If the rotor or other components are contaminated with toxic or patho-
genic materials, follow all appropriate safety and decontamination
procedures as outlined by your laboratory safety officer.
3In U.S., contact Nuclear Associates (New York); in Eastern Europe and Commonwealth States, contact Victoreen GmbH (Munich);
in South Pacific, contact Gammasonics Pty. Ltd. (Australia); in Japan, contact Toyo Medic Co. Ltd. (Tokyo).
4In U.S., contact Biodex Medical Systems (Shirley, New York); internationally, contact the U.S. office to find the dealer closest to you.

19
JA-25.50 Rotor
STERILIZATION AND DISINFECTION
• The rotor and all rotor components can be autoclaved at 121°C for
up to an hour. Remove the lid from the rotor and place the rotor,
lid, and spacers in the autoclave upside down.
•Ethanol(70%)
5or hydrogen peroxide (6%) may be used on all
rotor components, including those made of plastic. Bleach (sodium
hypochlorite) may be used, but may cause discoloration of anod-
ized surfaces. Use the minimum immersion time for each solution,
per laboratory standards.
While Beckman Coulter has tested these methods and found that they
do not damage the rotor or components, no guarantee of sterility or
disinfection is expressed or implied. When sterilization or disinfec-
tion is a concern, consult your laboratory safety officer regarding
proper methods to use.
Refer to publication IN-192, included in each box of tubes or bottles,
for tube and bottle sterilization and disinfection procedures.
STORAGE
When the rotor is not in use, store it in a dry environment (not in the
instrument) with the lid removed to allow air circulation so moisture
will not collect in the tube cavities.
5Flammability hazard. Do not use in or near operating centrifuges.

20
JA-25.50 Rotor
RETURNING A ROTOR
Before returning a rotor or accessory for any reason, prior permission
(a Returned Goods Authorization form) must be obtained from
Beckman Coulter, Inc. This RGA form, which may be obtained from
your local Beckman Coulter sales office, should contain the following
information:
• rotor serial number,
• history of use (approximate frequency of use),
• reason for the return,
• original purchase order number, billing number, and shipping
number, if possible,
• name and phone number of the person to be notified upon receipt
of the rotor or accessory at the factory, and
• name and phone number of the person to be notified about repair
costs, etc.
To protect our personnel, it is the customer’s responsibility to ensure
that the parts are free from pathogens and/or radioactivity. Steriliza-
tion and decontamination must be done before returning the parts.
Smaller items (such as tubes, bottles, etc.) should be enclosed in a
sealed plastic bag.
All parts must be accompanied by a note, plainly visible on the out-
side of the box or bag, stating that they are safe to handle and that
they are not contaminated with pathogens or radioactivity. Failure to
attach this notification will result in return or disposal of the items
without review of the reported problem.
Use the address label printed on the RGA form when mailing the
rotor and/or accessories to:
Beckman Coulter, Inc.
1050 Page Mill Road
Palo Alto, CA 94304
Attention: Returned Goods
Customers located outside the United States should contact their local
Beckman Coulter office.
RGA