Nippon Genetics Europe FastGene User manual

FastGene
®
High Speed Mini Centrifuge
Cat.No.: NG003
2013 August / Rev. 01

HighSpeed Mini Centrifuge
2
CONTENTS
Page
1
.1
General Information 2
1.2
First QC Check 2
2.1
Packaging Content 2
3.1
Meanings of Symbols 3
3.2
Safety Precautions 3
4
Product Description & Technical Specifications 5
5
Installation 6
6
Operation 8
7
Maintenance 11
8
Transportation 11
9
Trouble Shooting 12
9.1
Error Codes 13
10
Rotor and Accessories 14
11
Ordering Information 14
12
Contact Information 15
1.1 GENERAL INFORMATION
Thank you for purchasing FastGene
®
High Speed Mini Centrifuge. This Manual includes the operation procedure. In order to
use the instrument properly, please read this manual carefully before using the instrument.
This centrifuge is designed for workstations in research laboratories in the fields of bioscience, medicine and chemistry. Twelve
1.5 ml or 2.0 ml micro test tubes can be centrifuged simultaneously in a 45°fixed-angle rotor.
1.2 FIRST QC CHECK
Please check the instrument and the packing list when you open the package. If you find there is something wrong with the
instrument or the packaging content, please contact Nippon Genetics Europe or your distributor in your country.
2.1 PACKAGING CONTENT
FastGene
®
High Speed Mini Centrifuge NG003
12 x 1.5/2.0 ml tube rotor 1
Rotor nut 1
Rotor lid 1
Adaptors for 0.5 ml tubes 12
Adaptors for 0.2 ml tubes 12
Operation Manual 1
Power Cord 1

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3.1. MEANINGS OF SYMBOLS & SAFETY PRECAUTIONS
Symbols on the device
Symbol Meaning Symbol Meaning
Indicate a hole for
manual door opening in
case of emergency
Attention and warning for
electric shock
Attention and warning for
rotor coupling
Attention and warning for
door opening and closing
Symbols in this document
Symbol Meaning Symbol Meaning
This symbol refers to safety
relevant warnings and indicates
possible dangerous outcomes
☞ Note.
This symbol refers to the important
reminder
3.2. SAFETY PRECAUTIONS
Before using the instrument, please read this operation manual to ensure correct usage. Incorrect handling of the instrument
could possibly result in personal injury or physical damage on the instrument or its accessories.
1. ALWAYS locate the instrument on a flat, rigid and stable table capable of withstanding the weight of the instrument and its
spinning operation.
2. ALWAYS make a safety zone of 30 cm around the centrifuge to indicate that neither hazardous materials nor persons
should be permitted within the area during operation. ALWAYS position the instrument with enough space on each side of
instrument to ensure proper air circulation.
3. ALWAYS install the instrument within a temperature and humidity controlled environment. (Permissible ambient
temperature: +5°C ~ +35 °C, Relative humidity: ≤85%)
4. Before connecting the power, check the rated voltage.
5. Should not use unapproved rotors and associated accessories. Only use rotors from NIPPON GENETICS EUROPE with
appropriate centrifugal tubes and suitable adaptors to embrace sample containers tightly enough inside rotors.
6. Before operating the instrument, check if the rotor and the rotor lid are securely fastened. Should operate the instrument
with a rotor properly installed and secured to the motor shaft.
7. Mount the rotor on the motor shaft properly, check it with spinning manually.
8. Do not stop the rotor by touching with hand during the instrument is running.
9. Emergency door open should be performed only when spinning is completely stopped.

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10. Should not exceed the rated speed or specific gravity. Samples whose density is greater than 1.2g/ml must have reduced
maximum rotational speed to avoid rotor failure.
11. The sample content should not exceed 80% of total capacity of a tube. Otherwise, it would cause spillage of sample fluid
and even the tube breakage.
12. ALWAYS load the tubes symmetrically with evenly weighted samples to avoid rotor imbalance. If necessary, use the water
blank to counterbalance the unpaired sample.
13. The operation speed should not exceed the highest value of the individual guaranteed g-forces of each centrifuge, rotor,
bucket or adaptors and sample container, especially the guaranteed g-force of sample container should not be neglected.
14. The rotors should be cleaned and kept dry after every use for longer life and safety.
15. ALWAYS disconnect the power supply prior to maintenance care and service to avoid electrical shock.
16. ALWAYS use proven disinfection procedures after centrifuging bio hazardous materials.
17. Should not centrifuge flammable, toxic, radioactive, explosive, or corrosive materials.
18. When it is necessary to use toxic or radioactive materials or pathogenic micro-organisms which belong to the Risk Group II
of WHO: “Laboratory Bio- safety Manual,” should follow national regulations.
Do not place dangerous materials within 30 cm distance around the instrument, and that is also
recommended by IEC 61010-2-020.
Use the emergency door open function only when the [Door] button on the control panel is dumb under
the condition of complete stop of rotor running.
Never try to open or move the instrument if it is not completely stopped.
If the power input is more than +/- 10% of the recommended voltage or fluctuates frequently, it may
cause malfunction of the instrument and often result serious damage.
Install the instrument at the place without any kinds of corrosive gases.

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4. PRODUCT DESCRIPTION & TECHNICAL SPECIFICATIONS
Max. RPM/RCF
(Rotor)
13,500 rpm/ 12,300 xg
(Fixed Angle)
6,000 rpm/ 1,850 xg
(PCR Strip Rotor)
(optional accessory)
Max. capacity 12 x 2.0 ml or 1.5 ml Tubes 4 x 8-well PCR Strips
Time control Pulse or timed ≤30 min
RPM/RCF conversion Yes
Noise level (dB) ≤56
Acc/Dec (sec) ≤12 / < 16
Display Blue LCD
Automatic door release at completion Yes
Power supply (V/Hz) 220/50~60 (110V optional)
Power requirement(VA) 110
Dimension (W x D x H, mm) 208 x 245 x 145
Weight without rotor (kg) 4.4
CE mark Yes
Cat. No. NG003

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6
5. INSTALLATION
Power On/Off
1. Connect the AC Power cord at the power socket on the back of the instrument.
2. Turn on the instrument by pressing the switch on the back of the instrument.
Door Release
1. For opening the door, press the [DOOR] button.
The door is automatically opened after completion of spinning operation with
beeping sound.
When you close the door, close it until hearing clank shut.
☞
The door is not opened while the instrument is running.
If the door is opened, the instrument could not be operated even with pressing the [Start] button.
Power Failure: If there is any power failure during the operation, door is not opened with [Door] button. Door
can be opened only when the operation is completely stopped and the power is on again. If you want to
open the door at the power failure, please refer to 6. Emergency Door Open (page 10).
Rotor Coupling and Disassembling
Before coupling a rotor, clean the motor shaft and chamber with soft dry towel.
Fixed Angle Rotor
1. Mount a proper rotor into the motor shaft.
2. Place the Rotor Locking Nut at the center hole of the rotor.


To assemble the rotor: Rotate the Rotor Locking Nut clockwise
until tightly assembled.


To disassemble the rotor: Rotate the Rotor Locking Nut counterclockwise.
3. After loading sample tubes, close the rotor lid until hearing clank shut.


To open the lid, lift the nut.

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8-well strip Rotor
1. Mount a proper rotor into the motor shaft.
2. Place the Rotor Locking Nut at the center hole of the rotor.


To assemble the rotor: Rotate the Rotor Locking Nut clockwise until tightly assembled.


To disassemble the rotor: Rotate the Rotor Locking Nut counterclockwise.
When the PCR rotor is coupled, please do not speed over 6,000 rpm / 2,400 rcf,
as mentioned on the rotor.
Positioning of Sample Tubes
1. Before loading sample tubes, check the water drop or dirt in the rotor hole or inner adaptor.
2. If there is a water drop or dirt in the rotor hole or inner adaptor, remove it with soft dry cloth.
3. Tubes should be placed in the rotor with same amount of samples at symmetrical positions.
4. Only use appropriate centrifugal tubes and do not exceed the speed beyond the tube’s max g-force.
5. For safety, fill the sample for 70~80% in the tubes.
Correct Ways of Sample Balancing & Tube Usage
☞ If the number of samples is not in pair, please load the control tubes at each symmetrical position. Otherwise, it results
noise and vibration, which eventually damage the instrument.

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6. OPERATION
Display Window
Shows speed, time, status of running and the status of door opening or closing.
RPM/RCF Modes are displayed as rpm ot rcf.
While running, is flickering.
appears when the Door is opened and appears when the Door is closed.
Time is displayed as ‘min’.
Setting Buttons
When setting up the RPM/RCF and Time, you can put the set value with up (▲) and down (▼) button.
Function Buttons
[Door] For opening instrument door
[Pulse] For quick spin down
 [Start/Stop] Commend start and stop operation
Setting the RPM/RCF Value
Maximum RPM/RCF: 13,500 RPM/ 12,300 x g
Display value: multiply 1,000 to check real value. (Example: RPM Display value 13.5 indicates RPM 13,500)

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Setting the RPM Value
1. Press the [RPM/RCF] button once to adjust RPM.
RPM LED is flickering on the display window, indicating that the instrument is in the RPM Mode.
2.Press the [ ▲▼ ] buttons to change input value.
RPM setting unit: 0.1 unit (0.1=100rpm)
After 5 seconds from pressing the input value, the setting value is saved.
If you want to check the input value, press [RPM/RCF] button.
If you do not press the [ ▲▼ ] button for 5 seconds, the setting mode is cleared.
Setting the RCF Value
1. Press the [RPM/RCF] button twice to adjust RCF.


RCF LED is flickering on the display window, indicating that the instrument is in the RCF Mode.
2. Press the [▲▼] buttons to change input value.


RCF setting unit: 0.1 unit (0.1=100 rcf)


After 5 seconds from pressing the input value, the setting value is saved.


If you want to check the input value, press [RPM/RCF] button.


If you do not press the [▲▼] button for 5 seconds, the setting mode is cleared.
When the PCR rotor is coupled, please keep in mind that do not over speed at the max. 6,000 rpm/2,400 rcf,
as mentioned on the rotor.

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10
Setting the Time Value
Time control: 1 min. ~30 min ; Time setting unit: 1min
1. Press the [TIME] button once.
‘min’ on LED is flickering.
2. Press the [▲▼] buttons to change input value.
After 5 seconds from pressing the input value, the setting value is saved.
If you do not press the [▲▼] button for 5 seconds, the setting mode is cleared.
Start/Stop
1. After setting RPM/RCF and Time, press [Start/Stop] button.
The running starts only when the door is closed.
In case of pressing the [Start/Stop] button while running, the running is
stopped.
Pulse
Button for quick spin down
1. If you press [Pulse] button and release at the point you want to stop, the centrifuge
decelerates immediately.
When the running is stopped, the door is opened automatically with beeping
sound.
Emergency Door Open
The door can be unlocked manually with Emergency Door Open Function.
1. After pulling the instrument forward about 10 cm, find the “Emergency Door Open Hole”
at the bottom of the instrument
2. Insert spikes (a car key, scissors, etc.) 2~3 cm into the “Emergency Door Open Hole”
and pull the spikes at the opposite direction of the arrow.
Manual opening should be performed only when spinning is completely stopped. Otherwise, harmful
damage will be accompanied to not only operators but samples.
After opening the door manually, it is recommended to wait until normal electricity comes back.

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11
7. MAINTENANCE
Outer part of Instrument
1. Clean the outside of the instrument with dry soft cloth. If necessary, dip the cloth in neutral detergent and clean
contaminated area. Keep completely dry after cleaning.
2. Do not use any volatile chemicals such as alcohol and benzene, etc.
3. Be careful not to make scratches on the surface of the instrument. The scratches can cause corrosion on the surface of the
instrument. If any rust appears, clean it with neutral detergents and keep dry.
Chamber
1. Keep dry inside the chamber after every use.
2. If the chamber is contaminated, dip the cloth in neutral detergent and clean contaminated area.
Shaft
1. Always make special attention to clean the motor shaft to avoid any imbalance problem due to the contaminants.
2. After using the instrument, take out the rotor from the shaft, and clean the shaft with dry soft cloth to keep dry.
Rotor
1. If any parts are contaminated with samples, clean the rotor with soft wet cloth and keep the rotor dry.
2. Be careful not to make scratches inside or on the surface of rotors. Any small scratches can cause corrosion of the rotor
and big damage to the instrument.
3. If you do not use the instrument, keep the rotor separately from the motor shaft and stand it upside down.
8. TRANSPORTATION OF THE INSTRUMENT
1. If you need to move or ship the instrument, be cautious to protect the motor shaft from any physical impact or turbulence.
2. Do not mount a rotor in any cases of movement. Fill inside the chamber with proper materials to keep the motor shaft on
place and not to be influenced by physical pressure.

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9. TROUBLE SHOOTING
Symptom Check list
Power failure
Connect the AC Power cord and make sure that the line is completely
connected between the instrument and power outlet. Check the power switch
is turned on. (Please refer to 5. Power On/Off).
Can’t be started
If the door is not closed completely, the instrument can’t run.
Check the Door LED on the display window and close the door completely.
Can’t open the door
If the power is out, check the main fuse for the laboratory to supply the
power. If it is not solved in shortly, open the door with spikes for safety of
sample. (Please refer to 6. Emergency Door Open).
Can’t close the door
Remove the dirt at the door latch and then close the door completely again. If
the door seems not being closed by mechanical reason, please contact our
service team.
Noise and vibration during
running
Please check the balanced status of both the table and the instrument.
Please re-check the coupling status of the following three matches to
minimize the noise
1. the balanced way of coupling of the rotor into the motor shaft.
2. the completeness of fixing of the Rotor Locking Nut on the rotor.
3. the matching status of Rotor Lid with the rotor.
Check balances of samples in the rotor. (Please refer to 5. Positioning of
Sample Tubes) and load the same weight of samples symmetrically.

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13
9.1 ERROR CODES
If the instrument shows the error code with beeping sound, press [Stop] button to stop the beeping sound and press [Enter]
button to release of the error status and make the instrument go to the default setting again.
Error Possible Causes Actions
Error 1 or
Error 9 RPM Sensor
Shut off the power supply, and then, turn on the power switch again to check the
instrument.
If the error code shows continuously although you try to operate again, please call
NIPPON GENETICS EUROPE or your local distributor.
Error 2 Door
If the door is not closed completely, this message is appeared.
Remove the dirt at the door latch and then close the door completely again. Check the
Door LED on the display window. If it is not solved in shortly, open the door with
emergency door tool manually for safety of sample. (Please refer to 6. Emergency
Door Open.)
Error 3 Motor
Overheating
If the motor is overheated, this message is appeared.
Shut off the power supply for an hour, and then turn on the power switch for checking
the instrument.
If the error code shows continuously, please call NIPPON GENETICS EUROPE or
your local distributor.
Error 4 Low Voltage
If the power input of Power supply (V/Hz) is 10% less than required power, this
message is appeared.
Shut off the power supply and then check the voltage of the Power supply (V/Hz).
Use AVR (Automatic Voltage Regulation) to provide proper power.
Error 5 High Voltage
If the power input of Power supply (V/Hz) is 10% more than required power, this
message is appeared.
Shut off the power supply and then check the voltage of the Power supply (V/Hz).
Use AVR (Automatic Voltage Regulation) to provide proper power.
Error 6 Over Speed
If the instrument is spun with over speed, there will be some problems in the overload
of motor and the output of motor.
Shut off the power supply, and then, turn on the power switch again to check the
instrument.
Error 7 Software If the installed software has bugs, this message is appeared.
Tuning the firmware (Download)*
Error 8 Imbalance Check weight-balances of samples (Please refer to 5 Positioning of Sample Tubes)
and then turn off and on the instrument for checking.
* Any wire disconnection or tuning of the instrument must be performed only by a service engineer who is authorized by
NIPPON GENETICS Europe GmbH

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14
10. ROTORS AND ACCESSORIES
11. ORDERING INFORMATION
Product
Cat.No.
FastGene
®
HighSpeed Mini Centrifuge NG003
PCR Rotor for FastGene
®
HighSpeed Mini Centrifuge NG004
FastGene
®
1,5 ml Tubes (500) FG-015
FastGene
®
PCR Tubes 0.2 ml with domed caps (1000) FG-021D
FastGene
®
PCR Tubes 0.2 ml with flat caps (1000) FG-021F
8well PCR TubeStrips 0.2ml+single domed caps (120) FG-088WD
8well PCR TubeStrips 0.2ml+single flat caps (120)) FG-088WF
8well PCR TubeStrips 0.2ml+Domed CapStrips (120) FG-016DC
8well PCR TubeStrips 0.2ml+Flat CapStrips (120) FG-016FC

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15
12. CONTACT INFORMATION
Nippon Genetics Europe GmbH
Binsfelder Str. 77
D-52351 Düren
Germany
Phone: [phone]
Email: [e-mail]
[email protected]
For more detailed product information, contact details, questions, or trouble shooting please visit our English website
www.nippongenetics.eu or our German website www.nippongenetics.de.
FastGene
®
is a registered trademark of Nippon Genetics Europe GmbH
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