Okolab H201-T-UNIT-BL-CRYO User manual

H201-T-UNIT-BL-CRYO
Heating & Cooling Control Unit
Manual
IST2121_REV01
IST2122_REV01
IST2124_REV01
IST2125_REV01
SV 3.5.4C5-32.3053

Index
1PREFACE .....................................................................................................................................................................................1
2SYMBOL DESCRIPTION ..........................................................................................................................................................2
SYMBOLS USED IN THIS MANUAL ...............................................................................................................................................................2
SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................2
3SAFETY NOTES..........................................................................................................................................................................3
4SUPPLIED EQUIPMENT..........................................................................................................................................................5
5COMPATIBILITY.......................................................................................................................................................................6
6EQUIPMENT DESCRIPTION ..................................................................................................................................................7
7INSTALLATION .........................................................................................................................................................................8
HOW TO SETUP THE T-UNIT......................................................................................................................................................................8
HOW TO SETUP THE HEATING/COOLING BOX........................................................................................................................................9
HOW TO SETUP THE CALORFLEX TUBES...............................................................................................................................................10
HOW TO SETUP THE CHILLER .................................................................................................................................................................11
HOW TO SETUP THE CONDENSATE DRAINER.......................................................................................................................................14
7.5.1 How to configure the Condensate Drainer ......................................................................................................................15
HOW TO START UP THE SYSTEM .............................................................................................................................................................15
HOW TO SHUT DOWN THE SYSTEM ........................................................................................................................................................17
8USER INTERFACE .................................................................................................................................................................. 18
HOMEPAGE.................................................................................................................................................................................................18
8.1.1 How to enter the set point temperature ...........................................................................................................................18
8.1.2 Controller Status: colours led and meaning ....................................................................................................................19
SETTINGS....................................................................................................................................................................................................20
8.2.1 Temperature .................................................................................................................................................................................20
8.2.1.1 Control Mode ..................................................................................................................................................................................................... 21
8.2.1.2 Fan Speed ............................................................................................................................................................................................................ 23
8.2.1.3 Chiller....................................................................................................................................................................................................................24
8.2.1.4 Status ....................................................................................................................................................................................................................25
8.2.2 Touch Screen.................................................................................................................................................................................25
8.2.2.1 Options.................................................................................................................................................................................................................. 26
8.2.2.2 Brightness ........................................................................................................................................................................................................... 26
8.2.2.3 Visual Effects...................................................................................................................................................................................................... 27
8.2.2.4 Date & Time........................................................................................................................................................................................................ 27
8.2.2.5 Alarms...................................................................................................................................................................................................................28
8.2.2.6 Data Logging .....................................................................................................................................................................................................28
OVERVIEW..................................................................................................................................................................................................33
ICON AND GLANCE MODE VIEW.............................................................................................................................................................33
INFO PAGE ..................................................................................................................................................................................................33
9TOUCH SCREEN CALIBRATION ........................................................................................................................................ 35
10 CLEANING & MAINTENANCE......................................................................................................................................... 36
11 SUPPORT ............................................................................................................................................................................. 37
12 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 38
13 TROUBLESHOOTING........................................................................................................................................................ 39
14 FIGURE LIST........................................................................................................................................................................ 40
15 TABLE LIST ......................................................................................................................................................................... 41
16 MANUAL REVISION TABLE ............................................................................................................................................ 41

1
1Preface
The H201-T-UNIT-BL-CRYO, together with the H201-ENCLOSURE-CRYO, is designed to maintain all the
thermal conditions required around your microscope, thus enabling to carry out prolonged observations on
biological specimens. The system controls the temperature in the range from 10°C below the Room temperature
to 45°C.
Note ►The minimum temperature reached in the microscope enclosure is generally about 10°C below the
Room temperature at an air flow of 100 m3/h or higher. For a Room temperature of 24°C the minimum
temperature is 14°C. The value of the minimum temperature may vary with the dimensions and the shape of
the microscope enclosure and it can be affected by the power consumption of the devices placed inside it.
The system is composed of the enclosure surrounding the microscope, H201-ENCLOSURE-CRYO, which
design depends on the user’s microscope, the Temperature Control Unit, H201-T-UNIT-BL with the
Heating/Cooling Box, the Chiller, water circulation chiller for the cooling system, a fine gauge thermocouple to
monitor the temperature of a blank sample or Reference Well located in close proximity to the experimental one,
a thermistor to monitor the temperature inside the enclosure or the Room temperature. The Condensate Drainer,
a peristaltic pump, drains the water from the Heating/Cooling Box system and collects it into the Condensate
Vessel.
H201-T-UNIT-BL-CRYO is operated via the OKO-TOUCH touch screen control panel (to be ordered
separately). OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition, data logging is possible via SMART-BOX, via DATA-LOG Okolab software and via following
third-party Imaging software Okolab DATA LOG is an optional software allowing logging temperature data or
modification of selected parameters. DATA LOG can be installed on your PC; a mini USB connection to the
Temperature Control Unit allows data download.
Okolab recommends reading carefully this manual to familiarize yourself with the functions and the
operation of H201-T-UNIT-BL-CRYO before use.

2
2Symbol description
This paragraph describes the symbols used in this manual and on the product label.
Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully use the instrument.
Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.
This symbol indicates the product production date.
This symbol indicates the Manufacturer data.
This symbol warns you to read the user manual before starting the device.

3
3Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.
−The equipment must only be used as intended and as described in this Manual.
−Equipment should be operated only by technically qualified personnel.
−Do not start up the equipment if some of its parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Transport the equipment with care.
−Equipment and its internal parts can be damaged by dropping and by shock.
−Not following these instructions can result in damage or breakdown of the device and its
accessories.
−The products labels can be found on the bottom panel of the Main Box.
−Do not disassemble any part of the system.
−Do not use a volatile solvent such as paint thinner to clean the instrument, because
deformation or discoloration may occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Do not exceed voltage indicated in this manual and on the product label.
−Avoid excessive induction noise, static electricity and magnetic fields.
−Do not expose this instrument to rain or moisture.
−This device is not designed for use for medical applications.
−Power cord of unit should be unplugged from electrical outlet when left unused for a long
period of time.
−VENTILATION. Unit should be situated so that its location or position does not interfere
with proper ventilation. Neither the gas mixer nor stream destinations should be in poorly
ventilated areas.
−Unit should be situated away from heat sources such as open flames, radiators, heat
registers, stoves, or other appliances or processes that produce heat.
−Do not start up the equipment if the supply cable is damaged.
−Connect the equipment only to grounded mains power socket.
−Do not disconnect cables while in operation.
−Do not open the unit. Do not remove cover or back.
−Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric
shock, fire or malfunction.
−No user serviceable parts inside.
−Unit should never be used where it can fall or be pushed into water.
International caution symbol marks this device. It is important to read the “Safety
Notes” before installing, using and commissioning this device, as the notes contain
important information relating to safety and EMC. Not following these instructions
can result in damage or breakdown of the device and its accessories
We reserve the right to make technical variations.

4
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE
OF ANY PRODUCT.

5
4Supplied equipment
H201-T-UNIT-BL-CRYO includes:
1. T-Unit (pointer 1 in Figure 1) –Temperature Control Unit
2. Fine Gauge Thermocouple (pointer 3 in Figure 1). To measure specimen temperature.
3. NTC Thermistor (pointer 4 in Figure 1). To measure the temperature inside the enclosure.
4. Heating/Cooling Box (pointer 5 in Figure 1). To connect to the T-Unit for heating/cooling operation.
5. Calorflex Tubes (x4) (pointer 6 in Figure 1). To connect the Heating/Cooling Box to the microscope
enclosure.
6. Water Tubes (x2) (pointer 8 in Figure 1). To connect the Heating/Cooling Box to the Cooler.
7. Condensate Drainer (pointer 9 in Figure 1). Peristaltic pump to drain the condensate from the
Heating/Cooling Box system.
8. Condensate Vessel (pointer 10 in Figure 1). To collect the condensate.
9. Chiller (pointer 11 in Figure 1). Water circulation chiller for cooling system.
10. KRYO 30 –LZB 109 (5 l bottle), to add to water for the cooling mixture of the Chiller.
5
6
8
10
11
9
7
1
2
34
Figure 1. H201-T-UNIT-BL-CRYO equipment overview.

6
5Compatibility
H201-T-UNIT-BL-CRYO is compatible with:
•OKO-TOUCH (required, to be ordered separately) (see point 2 in Figure 1), touch screen interface to
be connected to T-Unit. It includes:
−Touch Screen Interface
−#1 RS232 Serial cable. To connect the Touch Screen to the unit.
−MINI-USB-OTG cable. To connect a USB pen to the OKO-TOUCH for data download.
•H201-ENCLOSURE-CRYO. Microscope enclosure (pointer 7 in Figure 1).
•DATA-LOG. Software used to log, view, and store data from a PC. It may be used to change parameters of
the controllers via PC. Data can be exported in .xlsx format.
Figure 2. DATA LOG software.
•SM-BL Smart Box. To store data, to operate the controllers via web/smartphone/tablet and to allow
remote assistance for service.

7
6Equipment Description
Figure 3 describes the front and the rear panel of T-Unit.
1. OKO-TOUCH RS232 serial port.
2. Power Input.
3. Heating/Cooling Box connector.
4. Air Flow Sensor connector.
5. Chiller RS232 serial port with Chiller RS232 -Cable embedded.
6. General Purpose Input Output (GPIO) port.
7. NTC Thermistor port.
8. Gauge Thermocouple port.
9. USB port.
2354 6 7 8
1
9
Figure 3. T-Unit Overview

8
7Installation
Follow the instructions below for the H201-T-UNIT-BL-CRYO setup:
How to setup the T-Unit
1. Connect the 8-pins cable between the port labeled “Control Unit” on the side of the Heating/Cooling
Box and the port labeled “Heating Box” on the rear panel of the T-Unit (see Figure 5).
2. Use the 3-pins –Flow Sensor Cable to connect the flow sensor attached on the blue side of
Heating/Cooling Box to the connector labeled “Air flow sensor”on the rear panel of the T-Unit (see
Figure 5).
3. Plug the NTC Thermistor to the port labeled ‘Ambient Temperature’ on the rear panel of the T-Unit
(see Figure 5). This temperature sensor must be placed in air close to the illumination pillar in
proximity to the microscope stage. This thermistor monitors the air temperature inside the cage. The
NTC thermistor can be also used to monitor the room temperature when working in Sample Feedback
Mode. In this case, you have to place it outside the cage.
4. The Fine Gauge Thermocouple (green) plugs into the green port labeled ‘Reference Temperature’
located on the rear panel of the T-Unit (see Figure 5). When operating the device in ‘Sample Feedback
Mode’ place this thermocouple in a Reference Well filled with water. Make sure that the end of the
Fine Gauge Thermocouple is fully immersed.
5. Connect the 24V-DC Power adapter to the “Power Input” port on the rear panel of the T-Unit.
Note ►It is strongly recommended to attach the NTC Thermistor close to the sample holder, on the side of
the stage or on the top condenser (use a standard adhesive tape to fix the sensor on those parts). Do not cover
the metal probe with the adhesive tape.
Adhesive
Tape
Thermistor
Figure 4. NTC Thermistor –position

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Electrical Connection
NTC Thermistor
Sensor cable
Heating/Cooling
Box
Fine gauge
thermocouple
8-pins
cable
3-pins
cable
Figure 5. T-Unit - Connection
6. Connect the touch screen interface OKO-TOUCH to the T-Unit using the serial cable provided (see
Figure 6).
OKO-TOUCH
T-UNIT
Figure 6.OKO-TOUCH Connection
How to setup the Heating/Cooling Box
Make sure that the Heating/Cooling Box –Cable (see pointer 1 in Figure 7) is connected.
Plug the power cord to the “Power Input” port on the side of the Heating/Cooling Box.

10
1
2
Figure 7. Heating/Cooling –setup.
How to setup the Calorflex Tubes
Use the Calorflex Tubes to connect the H201-T-UNIT-BL-CRYO to the microscope enclosure:
1. Attach the two-red marked Calorflex Tubes to the red side of the Heating/Cooling Box and to the
hoses, placed on the back panel of the microscope enclosure (see Figure 8).
2. Attach the two-blue marked Calorflex Tubes to the blue side of the Heating/Cooling Box and to the
hoses, placed on the top panel of the microscope enclosure (see Figure 8).
Figure 8. Calorflex Tubes –setup.
Note ►To connect the Calorflex Tubes to the Heating/Cooling Box, insert the hose bayonet connector ring
that is on the end part of the tubes on the hose bayonet connector flange mounted on the blue/red side of the
Heating/Cooling Box. Then rotate the bayonet connector ring until the screws do not fit into their housings.
Repeat the same procedure for the microscope enclosure connectors.

11
How to setup the Chiller
Use the Water Tubes to connect the Chiller to the Heating/Cooling Box.:
1. One of the ends of the red marked Water Tube is composed of 10 mm ID black butyl isolated tube
with a manual valve in the middle. Connect it to the color-related connector, the water inlet port, on
the rear panel of the Chiller. The other end has to be connected to the color-related push to fit
connector on the side panel of the Heating/Cooling Box (see Figure 9).
Chiller
Heating /
Cooling Box
Figure 9. red marked Water Tube –connection.
Make sure to strongly push the tubes all the way into push to fit connectors to avoid water leakage.
To remove tubing from the connectors, push the rubber ring while pulling the tubing.
If the tubing does not come out easily, do not force it, simply make sure the ring is pushed properly.
2. One of the ends of the blue marked Water Tube is composed of 10 mm ID black butyl isolated tube
with a manual valve in the middle. Connect it to the color-related connector, the water inlet port, on
the rear panel of the Chiller. The other end has to be connected to the color-related push to fit
connector on the side panel of the Heating/Cooling Box (see Figure 10).
Chiller
Heating /
Cooling Box
Figure 10. blue marked Water Tube –connection.

12
At first startup, check that the Water Tubes are connected properly to avoid any water leakage.
3. Make sure that the valves along the Water Tubes are in the opened position (see Figure 11, a).
CLOSED
OPENED
ba
Figure 11.Opened and closed position of the valve (a -b).
Note ►Close the valves before disconnecting the Water Tubes.
4. Prepare the cooling mixture adding 5 liters of KRYO 30 –LZB 109 to 5 liters of distilled water to obtain a
freezing temperature of about -15°C. The total amount of the cooling mixture should be about 10 liters.
Ensure that the water meets the required criteria. See Chiller’s User Manual (paragraph 4.4
“Cooling Water”).
5. Close the Drain Plug (see the Chiller’s User Manual pag. 16). Turn the Drain Plug clockwise as far as
the stop.
6. Fill the Chiller with the cooling mixture. Check the level indicator placed on the front panel of the
Chiller. The top arrow of the level indicator (see pointer 1 in Figure 12) indicates the maximum level
allowed by the Chiller. The bottom arrow (see pointer 2 in Figure 12) indicates the minimum
permissible level.

13
2
1
Figure 12. Chiller's Level Indicator
Do not exceed the maximum permissible level.
Note ►Monitor regularly the water level inside the Chiller from the Level Indicator. If the water level drops
too far, the Chiller automatically goes into alarm condition.
7. Connect the Chiller to the T-Unit using the Chiller RS232 –Cable already integrated in the T-Unit (see
Figure 13).
Chiller
Figure 13. Chiller RS232 serial connection

14
For the proper operation of the system, do not place anything on the top of the chiller (see Figure
14).
Figure 14. Correct setup of the chiller.
How to setup the Condensate Drainer
Follow the steps below to install the Condensate Drainer:
1. Connect the end of the yellow marked tube to the Draining Pump Tube Connector - push-to-fit
connector - on the side panel of the Cooling Block of the Heating/Cooling Box. Connect the other end
to the yellow marked inlet port of the Condensate Drainer by the provided connector (See Figure 15)
2. Connect the end of the green marked tube to the green marked outlet port of the Condensate Drainer
by the provided connector. Connect the other end to the push to fit connector of the Condensate Vessel
(See Figure 15).
Note ►Make sure to strongly push the tubes all the way into the connectors to avoid water leakage.

15
Heating /
Cooling Box
Condensate
Vessel
Condensate
Drainer
Figure 15. Condensate Drainer –assembly.
3. Connect the Condensate Drainer to the main power supply using its supplied power cord.
7.5.1 How to configure the Condensate Drainer
The Condensate Drainer has the dosing quantity parameter set by default at 10 8H. This means that the
Condensate Drainer doses 10ml of water every 8 hours. Okolab suggest changing the parameters to 20 1H to dose
20 ml of water every hour. For setting the dosing quantity, press button no.1 (1 ml -steps) and/or button no.2 (10
ml –steps) as often as the desired dosing quantity is indicated. For setting the interval time, press button no.3 as
often as the desired value is indicated.
Note ►If the system runs continuously in cooling mode, check the water content inside the Condensate Vessel
regularly. The Condensate Vessel should be emptied at least once a week. To empty the Condensate Vessel,
remove the green marked tube from the push to fit connector and unscrew the cup of the vessel. If the setpoint
temperature is higher than the Room temperature, there is no need to check the water level.
How to start up the system
To start up H201-T-UNIT-BL-CRYO, follow the instructions below:
Before starting up the Chiller, ensure that the bypass adjustment wheel is completely closed. (see
Chiller’s User Manual, pag. 16 or Figure 16). Turn the bypass adjustment wheel anticlockwise as far
as the stop.
1. Switch the Chiller on.

16
Check that no error message will be displayed on the Chiller’s Control Panel (see Chiller’s User
Manual, pag. 15).
2. Set the Chiller’s pump pressure using the bypass adjustment wheel placed on the rear panel of the
Chiller (see Figure 16).
Figure 16. Chiller’s bypass adjustment wheel.
To increase the pump pressure, turn the bypass adjustment wheel clockwise very slowly until a
pressure value of 1 barg is reached. To check the pressure value, monitor the manometer placed on
the front panel of the Chiller (see Figure 17).
Figure 17. Chiller’s Manometer.
Set the Chiller’s pump pressure at 1 barg. Do not exceed 1.3 barg.
Note ►Make sure that the valves along the Water Tubes are opened.
3. Set the lower limit value for the temperature of the Chiller at 2°C (see the Chiller’s User Manual pag.
39).
4. Switch the T-Unit on using the power button on OKO-TOUCH. A small green LED light will blink on
the front of the T-Unit. This means that the system is initializing.

17
5. Se the Setpoint temperature (see paragraph 8.1.1)
6. Switch the Condensate Drainer on.
Tip ►Wait until system has reached the steady state before loading your samples.
How to shut down the system
To shut down H201-T-UNIT-BL-CRYO follow the instructions below:
1. Switch the T-Unit off using the power button on OKO-TOUCH.
2. Switch the Condensate Drainer off.
3. Switch the Chiller off and let it cool down or heat up to Room temperature.
4. Close the valve along the Water Tube. Remove the end of the Water Tube from the connector on the
rear panel of the Chiller. Keep the Water Tube’s end vertical in order to avoid any water leakage.
Place the end of the Water Tube in a vessel and let the excess water flow. Open the valve and collect
the remain water. Repeat the operation for both Water Tubes.
5. Place a vessel under the drain plug placed on the rear panel of the Chiller and open the drain plug to
empty the Chiller.

18
8User Interface
This chapter describes the user interface of the H201-T-UNIT-BL-CRYO.
Homepage
124
3
5
8
9
10
7
6
Figure 18. Homepage of the H201-T-UNIT-BL-CRYO Controller Touch Screen Display
1. Status LED
2. Temperature set point (see paragraph 8.1.1 to learn how to change the set point value)
3. Temperature current value
4. Minimun and Maximum Temperature reached within the time frame set in the Options subpage
(see paragraph 8.2.2.1)
5. Chart history length set at 1 hour
6. Display mode. Touch here to switch display mode
7. Overview page
8. Settings. Press here to access system options and settings
9. Home. To open the homepage
10. Product info. Press here to know generic info about OKO TOUCH and H201-T-UNIT-BL and
running time
Tip ►The Touch Screen is pre-set at the following temperature: 37°C. Once turning the Touch Screen on it
will start operating to reach this set-point value.
8.1.1 How to enter the set point temperature
To input a new set-point temperature, touch the corresponding tab, as indicated in Figure 19 a.