Okolab H201-T-UNIT-BL-CRYO User manual

H201-T-UNIT-BL-CRYO
Heating & Cooling Control Unit
Manual
IST2121_REV01
IST2122_REV01
IST2124_REV01
IST2125_REV01
SV 3.5.4C5-32.3053

Index
1PREFACE .....................................................................................................................................................................................1
2SYMBOL DESCRIPTION ..........................................................................................................................................................2
SYMBOLS USED IN THIS MANUAL ...............................................................................................................................................................2
SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................2
3SAFETY NOTES..........................................................................................................................................................................3
4SUPPLIED EQUIPMENT..........................................................................................................................................................5
5COMPATIBILITY.......................................................................................................................................................................6
6EQUIPMENT DESCRIPTION ..................................................................................................................................................7
7INSTALLATION .........................................................................................................................................................................8
HOW TO SETUP THE T-UNIT......................................................................................................................................................................8
HOW TO SETUP THE HEATING/COOLING BOX........................................................................................................................................9
HOW TO SETUP THE CALORFLEX TUBES...............................................................................................................................................10
HOW TO SETUP THE CHILLER .................................................................................................................................................................11
HOW TO SETUP THE CONDENSATE DRAINER.......................................................................................................................................14
7.5.1 How to configure the Condensate Drainer ......................................................................................................................15
HOW TO START UP THE SYSTEM .............................................................................................................................................................15
HOW TO SHUT DOWN THE SYSTEM ........................................................................................................................................................17
8USER INTERFACE .................................................................................................................................................................. 18
HOMEPAGE.................................................................................................................................................................................................18
8.1.1 How to enter the set point temperature ...........................................................................................................................18
8.1.2 Controller Status: colours led and meaning ....................................................................................................................19
SETTINGS....................................................................................................................................................................................................20
8.2.1 Temperature .................................................................................................................................................................................20
8.2.1.1 Control Mode ..................................................................................................................................................................................................... 21
8.2.1.2 Fan Speed ............................................................................................................................................................................................................ 23
8.2.1.3 Chiller....................................................................................................................................................................................................................24
8.2.1.4 Status ....................................................................................................................................................................................................................25
8.2.2 Touch Screen.................................................................................................................................................................................25
8.2.2.1 Options.................................................................................................................................................................................................................. 26
8.2.2.2 Brightness ........................................................................................................................................................................................................... 26
8.2.2.3 Visual Effects...................................................................................................................................................................................................... 27
8.2.2.4 Date & Time........................................................................................................................................................................................................ 27
8.2.2.5 Alarms...................................................................................................................................................................................................................28
8.2.2.6 Data Logging .....................................................................................................................................................................................................28
OVERVIEW..................................................................................................................................................................................................33
ICON AND GLANCE MODE VIEW.............................................................................................................................................................33
INFO PAGE ..................................................................................................................................................................................................33
9TOUCH SCREEN CALIBRATION ........................................................................................................................................ 35
10 CLEANING & MAINTENANCE......................................................................................................................................... 36
11 SUPPORT ............................................................................................................................................................................. 37
12 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 38
13 TROUBLESHOOTING........................................................................................................................................................ 39
14 FIGURE LIST........................................................................................................................................................................ 40
15 TABLE LIST ......................................................................................................................................................................... 41
16 MANUAL REVISION TABLE ............................................................................................................................................ 41

1
1Preface
The H201-T-UNIT-BL-CRYO, together with the H201-ENCLOSURE-CRYO, is designed to maintain all the
thermal conditions required around your microscope, thus enabling to carry out prolonged observations on
biological specimens. The system controls the temperature in the range from 10°C below the Room temperature
to 45°C.
Note ►The minimum temperature reached in the microscope enclosure is generally about 10°C below the
Room temperature at an air flow of 100 m3/h or higher. For a Room temperature of 24°C the minimum
temperature is 14°C. The value of the minimum temperature may vary with the dimensions and the shape of
the microscope enclosure and it can be affected by the power consumption of the devices placed inside it.
The system is composed of the enclosure surrounding the microscope, H201-ENCLOSURE-CRYO, which
design depends on the user’s microscope, the Temperature Control Unit, H201-T-UNIT-BL with the
Heating/Cooling Box, the Chiller, water circulation chiller for the cooling system, a fine gauge thermocouple to
monitor the temperature of a blank sample or Reference Well located in close proximity to the experimental one,
a thermistor to monitor the temperature inside the enclosure or the Room temperature. The Condensate Drainer,
a peristaltic pump, drains the water from the Heating/Cooling Box system and collects it into the Condensate
Vessel.
H201-T-UNIT-BL-CRYO is operated via the OKO-TOUCH touch screen control panel (to be ordered
separately). OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition, data logging is possible via SMART-BOX, via DATA-LOG Okolab software and via following
third-party Imaging software Okolab DATA LOG is an optional software allowing logging temperature data or
modification of selected parameters. DATA LOG can be installed on your PC; a mini USB connection to the
Temperature Control Unit allows data download.
Okolab recommends reading carefully this manual to familiarize yourself with the functions and the
operation of H201-T-UNIT-BL-CRYO before use.

2
2Symbol description
This paragraph describes the symbols used in this manual and on the product label.
Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully use the instrument.
Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.
This symbol indicates the product production date.
This symbol indicates the Manufacturer data.
This symbol warns you to read the user manual before starting the device.

3
3Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.
−The equipment must only be used as intended and as described in this Manual.
−Equipment should be operated only by technically qualified personnel.
−Do not start up the equipment if some of its parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Transport the equipment with care.
−Equipment and its internal parts can be damaged by dropping and by shock.
−Not following these instructions can result in damage or breakdown of the device and its
accessories.
−The products labels can be found on the bottom panel of the Main Box.
−Do not disassemble any part of the system.
−Do not use a volatile solvent such as paint thinner to clean the instrument, because
deformation or discoloration may occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Do not exceed voltage indicated in this manual and on the product label.
−Avoid excessive induction noise, static electricity and magnetic fields.
−Do not expose this instrument to rain or moisture.
−This device is not designed for use for medical applications.
−Power cord of unit should be unplugged from electrical outlet when left unused for a long
period of time.
−VENTILATION. Unit should be situated so that its location or position does not interfere
with proper ventilation. Neither the gas mixer nor stream destinations should be in poorly
ventilated areas.
−Unit should be situated away from heat sources such as open flames, radiators, heat
registers, stoves, or other appliances or processes that produce heat.
−Do not start up the equipment if the supply cable is damaged.
−Connect the equipment only to grounded mains power socket.
−Do not disconnect cables while in operation.
−Do not open the unit. Do not remove cover or back.
−Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric
shock, fire or malfunction.
−No user serviceable parts inside.
−Unit should never be used where it can fall or be pushed into water.
International caution symbol marks this device. It is important to read the “Safety
Notes” before installing, using and commissioning this device, as the notes contain
important information relating to safety and EMC. Not following these instructions
can result in damage or breakdown of the device and its accessories
We reserve the right to make technical variations.

4
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE
OF ANY PRODUCT.

5
4Supplied equipment
H201-T-UNIT-BL-CRYO includes:
1. T-Unit (pointer 1 in Figure 1) –Temperature Control Unit
2. Fine Gauge Thermocouple (pointer 3 in Figure 1). To measure specimen temperature.
3. NTC Thermistor (pointer 4 in Figure 1). To measure the temperature inside the enclosure.
4. Heating/Cooling Box (pointer 5 in Figure 1). To connect to the T-Unit for heating/cooling operation.
5. Calorflex Tubes (x4) (pointer 6 in Figure 1). To connect the Heating/Cooling Box to the microscope
enclosure.
6. Water Tubes (x2) (pointer 8 in Figure 1). To connect the Heating/Cooling Box to the Cooler.
7. Condensate Drainer (pointer 9 in Figure 1). Peristaltic pump to drain the condensate from the
Heating/Cooling Box system.
8. Condensate Vessel (pointer 10 in Figure 1). To collect the condensate.
9. Chiller (pointer 11 in Figure 1). Water circulation chiller for cooling system.
10. KRYO 30 –LZB 109 (5 l bottle), to add to water for the cooling mixture of the Chiller.
5
6
8
10
11
9
7
1
2
34
Figure 1. H201-T-UNIT-BL-CRYO equipment overview.

6
5Compatibility
H201-T-UNIT-BL-CRYO is compatible with:
•OKO-TOUCH (required, to be ordered separately) (see point 2 in Figure 1), touch screen interface to
be connected to T-Unit. It includes:
−Touch Screen Interface
−#1 RS232 Serial cable. To connect the Touch Screen to the unit.
−MINI-USB-OTG cable. To connect a USB pen to the OKO-TOUCH for data download.
•H201-ENCLOSURE-CRYO. Microscope enclosure (pointer 7 in Figure 1).
•DATA-LOG. Software used to log, view, and store data from a PC. It may be used to change parameters of
the controllers via PC. Data can be exported in .xlsx format.
Figure 2. DATA LOG software.
•SM-BL Smart Box. To store data, to operate the controllers via web/smartphone/tablet and to allow
remote assistance for service.

7
6Equipment Description
Figure 3 describes the front and the rear panel of T-Unit.
1. OKO-TOUCH RS232 serial port.
2. Power Input.
3. Heating/Cooling Box connector.
4. Air Flow Sensor connector.
5. Chiller RS232 serial port with Chiller RS232 -Cable embedded.
6. General Purpose Input Output (GPIO) port.
7. NTC Thermistor port.
8. Gauge Thermocouple port.
9. USB port.
2354 6 7 8
1
9
Figure 3. T-Unit Overview

8
7Installation
Follow the instructions below for the H201-T-UNIT-BL-CRYO setup:
How to setup the T-Unit
1. Connect the 8-pins cable between the port labeled “Control Unit” on the side of the Heating/Cooling
Box and the port labeled “Heating Box” on the rear panel of the T-Unit (see Figure 5).
2. Use the 3-pins –Flow Sensor Cable to connect the flow sensor attached on the blue side of
Heating/Cooling Box to the connector labeled “Air flow sensor”on the rear panel of the T-Unit (see
Figure 5).
3. Plug the NTC Thermistor to the port labeled ‘Ambient Temperature’ on the rear panel of the T-Unit
(see Figure 5). This temperature sensor must be placed in air close to the illumination pillar in
proximity to the microscope stage. This thermistor monitors the air temperature inside the cage. The
NTC thermistor can be also used to monitor the room temperature when working in Sample Feedback
Mode. In this case, you have to place it outside the cage.
4. The Fine Gauge Thermocouple (green) plugs into the green port labeled ‘Reference Temperature’
located on the rear panel of the T-Unit (see Figure 5). When operating the device in ‘Sample Feedback
Mode’ place this thermocouple in a Reference Well filled with water. Make sure that the end of the
Fine Gauge Thermocouple is fully immersed.
5. Connect the 24V-DC Power adapter to the “Power Input” port on the rear panel of the T-Unit.
Note ►It is strongly recommended to attach the NTC Thermistor close to the sample holder, on the side of
the stage or on the top condenser (use a standard adhesive tape to fix the sensor on those parts). Do not cover
the metal probe with the adhesive tape.
Adhesive
Tape
Thermistor
Figure 4. NTC Thermistor –position