Optics11 Life PIUMA NANOINDENTER User manual

PIUMA NANOINDENTER
USER MANUAL

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CONTACT INFORMATION
Optics11 Life
[phone]
[email protected]
www.optics11life.com
VISITING ADDRESS
Optics11 Life
VU University Campus
W&N Building room O-236
De Boelelaan 1081
1081 HV Amsterdam
The Netherlands
SHIPPING ADDRESS
Optics11 Life
De Boelelaan 1081
1081 HV Amsterdam
The Netherlands
COMPANY INFORMATION
Optics11 B.V.
KvK/CC: 52469417
VAT: NL850459734B01
Amsterdam, NL
Please see our website
for more information
about our products.
www.optics11life.com

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CONTENTS
Revision history 6
Safety 7
Introduction 8
1. Installation 9
1.1 The Piuma Nanoindenter and its components 9
1.2 Connecting components 10
2. Preparing the setup for measurements 11
2.1 Starting the system 11
Powering up the system 11
Starting the software 11
2.2 Mounting a probe to the Piuma Nanoindenter 12
Selecting the right probe 12
Installing a probe 13
Connecting the probe 13
Front panel window 15
Configuring the probe in the software 16
Setting the correct indenter head 17

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2.3 Calibrating the optical signal 18
Automated optical signal calibration 19
Failed optical calibration –“Quadrature Scan failed” 21
2.4 Calibrating the probe geometrical factor 22
Saving Calibration Data 26
3. Using the Piuma Nanoindenter 27
3.1 Stage controls 27
3.2 Piezo controls 28
3.3 The Find surface procedure 28
Approaching the sample using the manual stage 29
Using the automated find-surface function 29
3.4 Configure experiment 32
Find Surface 34
Wait 34
Move 35
Single indentations 36
Matrix indentations 36
Saving configure experiment file 38
3.5 Modes of operation 38
D-mode 39
P-mode 39
I-mode 40
3.6 Dynamic mechanical analysis - DMA 41
3.7 Maintenance menu and PID settings and PID tuner 42
3.8 Saving measurements 43
3.9 Performing measurements 44
3.10 Results 45
Indentation results tab 45
Scan results tab 46
3.11 Data processing and analysis 48
4. Optimizing instrumental set up 50
4.1 Environment stability 50
Temperature 50

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Vibrations 50
Laminar flow cabinet 51
4.2 Sample stability 51
Sample temperature and integrity 51
Sample immobilization 52
4.3 Probe stability 52
Solution temperature 52
Air bubble 53
Suitable probe cantilever stiffness 53
Suitable probe tip size 54
Adhesion 54
5. Working principle of the Piuma Nanoindenter 55
5.1 Principle of operation 55
Interferometric displacement sensing 55
The Piuma optical indentation probes 56
5.2 The measurement and calculation of the Young’s modulus 57
Oliver and Pharr / Field and Swain 58
Hertzian contact 59
Effective vs. bulk Young’s Modulus 59
DMA Analysis 60
6. The OP1550 interferometer 61
6.1 Using the OP1550 interferometer 62
The Measurement, Wavelength, Demodulation and Settings menu 62
The Laser, ADC, Output, Modulation and Maintenance menu 63
Adjusting Laser Power to improve the signal to noise ratio 64
Live calibration 64
7. FAQ & troubleshoot 66
Changing fuses 67
Flowchart - Calibration 67

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REVISION HISTORY
Version 2.0
New document
December 6, 2016
Ernst Breel
Version 2.1
Update
March 14, 2017
Jakob Pyszkowski
Version 2.2
Update
March 21, 2017
Jakob Pyszkowski
Version 2.3
Update
March 29, 2017
Jakob Pyszkowski
Version 2.4
Update
May 16, 2017
Jakob Pyszkowski
Version 2.5
Update
July 18, 2017
Jakob Pyszkowski
Version 2.6
Update
June 20, 2018
Jakob Pyszkowski
Version 2.7
Update
November 7, 2018
Jakob Pyszkowski
Version 2.8
Update
May 1, 2019
Jakob Pyszkowski
Version 3.0
Update
October 8, 2019
Kevin Bielawski
Version 3.1
Update
February 26, 2020
Nikolai Born
Version 3.2
Update
March 11, 2020
Nikolai Born
Version 3.3
Update
June 18, 2020
Elias Labordus
Version 3.4
Update
October 13, 2020
Eric Rutjes, Nelda Antonovaite

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SAFETY
The OP1550 interferometer, part of the Piuma Nanoindenter, is equipped with a class 1M laser. The
laser light is coupled out via a fiber connector on the front panel and has a terminator on the back
panel. Do not view directly into the beam with optical instruments.
The OP1550 is equipped with a 220V/110V plug. Disconnect the instrument before changing the fuse
or before switching from 220V to 110V (or vice versa). Do not open the box, as this might result in
serious injuries.

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INTRODUCTION
This document describes the installation and operation of the Piuma Nanoindenter instrument, as
developed by Optics11 Life. Details of the installation of the Piuma Nanoindenter are provided in
Chapter 1. Chapter 2 describes the preparation of the Piuma Nanoindenter for experiments. How to
perform a measurement is described in detail in Chapter 3. Chapter 4 describes the optimization of
sample preparation and measurement conditions. Chapter 5 describes the Piuma Nanoindenter’s
working principle. Chapter 6 describes how to operate the OP1550 interferometer, and finally Chapter
7 provides a FAQ and alternative options for support by a flowchart for troubleshooting.
Figure 1: The Piuma Nanoindenter in action: measuring intact brain tissue.

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1. INSTALLATION
This section describes the installation of the Piuma Nanoindenter by providing an overview of the
Piuma Nanoindenter and its components, the considerations for finding a location to set up the
system, the wiring scheme for connecting the individual components and the actions required to start
up the Piuma Nanoindenter.
1.1 The Piuma Nanoindenter and its components
The Piuma Nanoindenter consists of five components: (1) The Piuma indenter head and (2) patchcord
adapter, the (3) Optics11 Life OP1550 interferometer, (4) the Piuma controller box, (5) the PC running
the Piuma Nanoindenter software suite and (6) the Optics11 Life optical nanoindentation probes.
Figure 2: Picture of the Piuma Nanoindenter setup showing the Piuma indenter head (right), OP1550 interferometer,
Piuma controller and the PC.
The Optics11 Life OP1550 interferometer operates independently of the other components and can
be switched on and off at any time. The Piuma controller box powers the Piuma indenter head and
communicates with the software running on the PC and should not be switched off before exiting the
Piuma nanoindentation software suite. The software will automatically detect and connect to Piuma
components connected to the PC. For the best user experience, it is recommended to first power on
all peripheral components before running the software.
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1.2 Connecting components
Together with the Piuma Nanoindenter the following cables are provided:
1x BNC connector cable (previous Piuma versions 2x BNC)
1x DVI signal cable
2x Power cable
1x PC power cable including adapter
2x USB (B-type) connector cable
50 ΩTerminator
1x Fiber patch-chord & adapter
When installing the system, please carefully follow the scheme below (Figure 3).
Figure 3: Schematic drawing of the connections that are required to set-up the Piuma Nano-indenter.
The controller box is connected to the computer using one of the two USB ‘HUB’ connectors on the
back of the Piuma controller, and a free USB slot on the PC (must be a USB3.0 port). The Piuma
indenter head is connected to the controller box using the original DVI cable supplied with the Piuma
system. The OP1550 readout is connected to the controller box using one BNC and one USB cable.
The BNC cable connects the ‘Output’ at the back of the OP1550 with the ‘Input’ on the back of the
controller box and the second USB cable connects the ‘USB’ output on the back of the OP1550 to
the second ‘HUB’ connector on the back of the controller box (Figure 3).
The optional backlight module requires 5V to operate. To power this module, connect the USB cable
to any free USB port on the PC or the controller box.
The optional inverted camera module requires 5V to operate and can be connected to the Piuma
through a free USB HUB port at the rear of the controller box or directly to a free USB port on the PC.