Optics11 Life PIUMA NANOINDENTER User manual

PIUMA NANOINDENTER
USER MANUAL

2www.optics11life.com
CONTACT INFORMATION
Optics11 Life
[phone]
[email protected]
www.optics11life.com
VISITING ADDRESS
Optics11 Life
VU University Campus
W&N Building room O-236
De Boelelaan 1081
1081 HV Amsterdam
The Netherlands
SHIPPING ADDRESS
Optics11 Life
De Boelelaan 1081
1081 HV Amsterdam
The Netherlands
COMPANY INFORMATION
Optics11 B.V.
KvK/CC: 52469417
VAT: NL850459734B01
Amsterdam, NL
Please see our website
for more information
about our products.
www.optics11life.com

3
CONTENTS
Revision history 6
Safety 7
Introduction 8
1. Installation 9
1.1 The Piuma Nanoindenter and its components 9
1.2 Connecting components 10
2. Preparing the setup for measurements 11
2.1 Starting the system 11
Powering up the system 11
Starting the software 11
2.2 Mounting a probe to the Piuma Nanoindenter 12
Selecting the right probe 12
Installing a probe 13
Connecting the probe 13
Front panel window 15
Configuring the probe in the software 16
Setting the correct indenter head 17

4www.optics11life.com
2.3 Calibrating the optical signal 18
Automated optical signal calibration 19
Failed optical calibration –“Quadrature Scan failed” 21
2.4 Calibrating the probe geometrical factor 22
Saving Calibration Data 26
3. Using the Piuma Nanoindenter 27
3.1 Stage controls 27
3.2 Piezo controls 28
3.3 The Find surface procedure 28
Approaching the sample using the manual stage 29
Using the automated find-surface function 29
3.4 Configure experiment 32
Find Surface 34
Wait 34
Move 35
Single indentations 36
Matrix indentations 36
Saving configure experiment file 38
3.5 Modes of operation 38
D-mode 39
P-mode 39
I-mode 40
3.6 Dynamic mechanical analysis - DMA 41
3.7 Maintenance menu and PID settings and PID tuner 42
3.8 Saving measurements 43
3.9 Performing measurements 44
3.10 Results 45
Indentation results tab 45
Scan results tab 46
3.11 Data processing and analysis 48
4. Optimizing instrumental set up 50
4.1 Environment stability 50
Temperature 50

5
Vibrations 50
Laminar flow cabinet 51
4.2 Sample stability 51
Sample temperature and integrity 51
Sample immobilization 52
4.3 Probe stability 52
Solution temperature 52
Air bubble 53
Suitable probe cantilever stiffness 53
Suitable probe tip size 54
Adhesion 54
5. Working principle of the Piuma Nanoindenter 55
5.1 Principle of operation 55
Interferometric displacement sensing 55
The Piuma optical indentation probes 56
5.2 The measurement and calculation of the Young’s modulus 57
Oliver and Pharr / Field and Swain 58
Hertzian contact 59
Effective vs. bulk Young’s Modulus 59
DMA Analysis 60
6. The OP1550 interferometer 61
6.1 Using the OP1550 interferometer 62
The Measurement, Wavelength, Demodulation and Settings menu 62
The Laser, ADC, Output, Modulation and Maintenance menu 63
Adjusting Laser Power to improve the signal to noise ratio 64
Live calibration 64
7. FAQ & troubleshoot 66
Changing fuses 67
Flowchart - Calibration 67