Orflo moxi z User manual

The information in this document is subject to change without notice and should not be construed as a
commitment by ORFLO Technologies, a DBA of E.I. Spectra, LLC. Neither Orflo Technologies nor any of
its affiliated corporations assumes responsibility for any errors that may appear in this document. This
manual is believed to be complete and accurate at the time of publication. In no event shall Orflo
Technologies or any of its affiliated corporations be liable for incidental or consequential damages in
connection with or arising from the use of this manual.
© 2012 ORFLO Technologies, a DBA of E.I. Spectra, LLC. All rights reserved.
Rev. 5.0.1, 03/2012
The ORFLO symbol and Moxi are trademarks of E.I Spectra, LLC, registered in the U.S. and other countries.
Windows, Windows XP, Windows Vista, and Windows 7 are trademarks of Microsoft Corporation, registered in the
U.S. and other countries.
Mac and Mac OS are trademarks of Apple Corporation, registered in the U.S. and other countries.
Bluetooth and the Bluetooth logos are registered trademarks of Bluetooth SIG, Inc., registered in the U.S. and other
countries.
Accutase is a registered trademark of Innovative Cell Technologies.
TÜV SÜD and the TÜV SÜD symbol are registered trademarks of TÜV SÜD Aktiengesellschaft.

Contents
Introduction ................................................................................................................................... 1!
Symbols Used in This User Guide................................................................................................ 1!
Safety Precautions........................................................................................................................ 2!
Consignes de sécurité .................................................................................................................. 3!
Moxi Z Mini Automated Cell Counter Kit....................................................................................... 4!
Moxi Z Mini Automated Cell Counter Accessories........................................................................ 5!
Installation..................................................................................................................................... 6!
General Guidelines ....................................................................................................................... 6!
Materials Required........................................................................................................................ 7!
Using the Moxi Z Automated Cell Counter ................................................................................... 7!
Settings ..................................................................................................................................... 7!
Cell Counting............................................................................................................................. 7!
Managing the Data.................................................................................................................... 8!
Retrieving and Deleting Data .................................................................................................. 11!
Moxi Z Performance Test Using Calibration Check Beads..................................................... 13!
Moxi Z Performance Test Using Electronic Calibration Cassette ........................................... 14!
Moxi Z Help ............................................................................................................................. 14!
Installing MoxiChart Software on a PC ................................................................................... 15!
Connecting to Moxi Z via Bluetooth with a PC........................................................................ 15!
Connecting to Moxi Z via USB (requires Moxi Z OS v4.0 or greater) ..................................... 17!
Installing MoxiChart Software on a Mac.................................................................................. 18!
Connecting to Moxi Z via Bluetooth with a Mac ...................................................................... 18!
Instrument Firmware and Software Upgrades ........................................................................ 18!
Updating Moxi Z Firmware via Bluetooth (Moxichart) ............................................................. 18!
Updating Moxi Z Firmware via USB (requires Moxi Z OS v4.0 or greater) ............................. 20!
Moxi Apps ............................................................................................................................... 21!
Troubleshooting .......................................................................................................................... 22!
Maintenance and Storage........................................................................................................... 24!

Specifications for the Moxi Z Automated Cell Counter ............................................................... 26!
Ordering Information................................................................................................................... 28!
Technical Service ....................................................................................................................... 29!
Warranty ..................................................................................................................................... 29!
Applicable Patents ...................................................................................................................... 30!

Moxi Z Mini Automated Cell Counter User Manual
Page 1
Introduction
The ORFLO Moxi Z Mini Automated Cell Counter performs cell count and size measurements
for particle sizes of 4 – 25 microns (Type M cassette) and 3 – 20 microns (Type S cassette). It
also provides an assessment of mammalian culture cell health using a proprietary software
algorithm to report the standardized Moxi Population Index (MPI). It combines the gold standard
Coulter Principle with a patented thin-film sensor technology to deliver highly accurate and
repeatable results in just 8 seconds (Type M cassette) or 15 seconds (Type S cassette).
The instrument is ultra-small and runs on a rechargeable battery, making it ideal for use in a
hood. Cell concentration, average cell volume, average cell diameter, and the Moxi Population
Index (MPI) are displayed for each sample. Test results are also displayed in the form of a
histogram. The Moxi Z unit can store approximately 500 histograms, and if desired, the data
may be downloaded to a PC or Mac via a Bluetooth wireless connection or via USB connectivity
(v 4.0 or greater).
The system is intended for research use only and has been tested with cell types that are
representative of those commonly used.
The Moxi Z Mini Automated Cell Counting system is designed and manufactured by:
Orflo Technologies, a DBA of E.I. Spectra, LLC
P.O. BOX 7438 / 260 Northwood Way
Ketchum, ID 83340
www.orflo.com
Symbols Used in This User Guide
The following symbols are used throughout this user guide and/or on product labels. The user
is responsible for operating the product in accordance with the indicated requirements:
Symbol / Symbole
Definition / Définition
Warning alerts you to actions that may cause personal injury or pose a
physical threat.
La mise en garde vous alerte des actions qui risquent de causer des
blessures corporelles ou de constituer une menace physique.
Do not discard with common solid waste at end of life. Segregate with
other waste electrical and electronic equipment (WEEE) and send to an
appropriate facility for recycling.
Affixed in accordance with European Council Directives 2004/108/EC,
(electromagnetic compatibility) and 2006/95/EC (safety requirements)

Moxi Z Mini Automated Cell Counter User Manual
Page 2
Safety tested and certified by TÜV SÜD®Product Service Division.
Safety Precautions
Please review and understand the safety instructions below before operating the Moxi Z Cell
Counter.
WARNINGS:
•To avoid danger of electric shock, do not install the instrument in an area with a high
humidity level, such as a greenhouse or an incubator. Refer to Operating Environmental
Conditions in Specifications section.
•Do not touch the USB cable or USB charging adapter with wet hands.
•To avoid a potential shock hazard, choose the correct plug configuration and make sure
that the USB cable and USB charging adapter are plugged securely into a properly
grounded AC power outlet. Make sure that the connection between the USB cable and
the instrument is secure.
•Always ensure that the power supply input voltage matches the voltage available in your
location.
•Do not use with flammable or explosive liquids.
•Do not immerse instrument body in liquid, or allow liquid to enter any part of the
instrument.
CAUTION:
•Do not expose instrument to vibrations. Vibrations may cause instrument malfunction or
damage.
•Do not autoclave or expose to high temperature.
•Use only authorized accessories (universal power adapters, USB cable).
•If the instrument is dropped and broken, disconnect the USB cable and contact Orflo
Technologies. Do not attempt to disassemble the instrument.

Moxi Z Mini Automated Cell Counter User Manual
Page 3
Consignes de sécurité
Veuillez lire et vous assurer de comprendre les consignes de sécurité ci-dessous avant d’utiliser
le compteur Moxi Z Cell.
AVERTISSEMENTS :
•Afin d’éviter tout danger de choc électrique, ne pas installer l’instrument dans un
endroit où le taux d’humidité est élevé, comme dans une serre ou un incubateur. Se
reporter à la section sur les spécifications en matière de conditions
environnementales d’exploitation.
•Ne pas toucher au câble USB ou à l’adaptateur de charge USB les mains mouillées.
•Afin d’éviter tout risque de choc électrique, choisir la configuration de prise
appropriée et s’assurer que le câble USB et l’adaptateur de charge USB sont bien
branchés dans une prise de courant CA mise à la terre. S’assurer que la connexion
entre le câble USB et l’instrument est bien établie.
•Toujours s’assurer que la tension d’entrée du bloc d’alimentation correspond à la
tension disponible dans votre lieu.
•Ne pas utiliser avec des liquides inflammables ou explosifs.
•Ne pas immerger le corps de l’instrument dans du liquide ou permettre à du liquide
de pénétrer dans l’instrument.
ATTENTION :
•Ne pas exposer l’instrument à des vibrations. Les vibrations peuvent causer le
dysfonctionnement de l’instrument ou des dommages à celui-ci.
•Ne pas autoclaver ou exposer à des températures élevées.
•Utiliser uniquement les accessoires autorisés (adaptateurs de courant universels,
câble USB).Si l’instrument est échappé et brisé, débrancher le câble USB et
communiquer avec Orflo Technologies. Ne pas tenter de désassembler l’instrument.

Moxi Z Mini Automated Cell Counter User Manual
Page 4
Moxi Z Mini Automated Cell Counter Kit
The Moxi Z Mini Automated Cell Counter kit includes the Moxi Z instrument, USB Cable, Power
Adapter (US and EU versions only), Cassette Dispenser, Calibration Check Beads, USB Flash
Drive with software/user guide and 25 Cassettes (2 tests per cassette).
Touch Screen Display
Cassette Tray Power/Reset Button USB Cable Port
Cassette
Test 2 Fill Port
Test 1 Fill Port
Cassette

Moxi Z Mini Automated Cell Counter User Manual
Page 5
Part Function
Touch Screen Display Allows user to interface with instrument. Displays all
information needed for operation. Displays test results and
histograms with curve fitting, gating, cell volume,
concentration, diameter, and Moxi Population Index (MPI).
Power/Reset Button Turns Moxi Z on and off.
Resets the unit when pressed and held for >5 seconds.
Cassette Tray Tray that needs to be pressed down for inserting cassette
USB Cable Port Connects instrument to USB cable.
Cassette Disposable used for loading samples. Each cassette
contains two ports thereby allowing for two samples to be
run per cassette.
Moxi Z Mini Automated Cell Counter Accessories
Cassette Dispenser USB Cable & Power Adapter USB Flash Drive with software
Calibration Check Beads Electronic Calibration Cassette (sold separately)

Moxi Z Mini Automated Cell Counter User Manual
Page 6
Part Function
Cassette Dispenser Stores up to 25 cassettes for convenient
dispensing
USB Cable Connects instrument to PC/Mac or power adapter
Power Adapter Connects USB cable to an AC outlet
(US and EU models only)
USB Flash Drive Stores Moxi Z software and user manual
Calibration Check Beads Polystyrene beads for confirming proper system
operation and calibration
Electronic Calibration Cassette Electronic cassette for verifying proper system
(sold separately) operation and calibration
Installation
The Moxi Z is shipped in a condition ready for initial use with the battery partially charged. If
necessary, the battery can be charged by inserting the USB cable into the USB Cable Port and
connecting the cable to a computer or power adapter. If using a power adapter, insert the plug
into an AC outlet (110/220 V). The battery will be fully charged in approximately four hours.
Note: For optimal cell counting results, perform cell counts with the USB cable disconnected.
Although the unit may be operated using AC power, this mode may cause undesirable electrical
noise which could result in less accurate small particle counting results and may affect the MPI.
When not in use, the Moxi Z should be connected to a charging source so that the battery will
maintain a continuous charge and be ready for immediate use. If a low battery warning appears
on the display, stop using the instrument and charge the battery or operate the Moxi Z using an
AC power source.
General Guidelines
The Moxi Z Mini Automated Cell Counter is used with Moxi Z Cassettes. Refer to the
Specifications section for information on the operating ranges for the system.
The sample volume for a test should be 75 µL.
If necessary, prepare dilutions of the sample using ORFLO Diluent (Cat. No. MXA006) or a
diluent compatible with the characteristics of the cells. The diluent used should not cause
changes in the cell size and should have sufficient conductivity to enable operation of the
instrument. Water, hypotonic, or hypertonic solutions are not acceptable diluents.