Orphee Mythic 18 Vet User manual

MYTHIC 18
Copyright© Orphée SA. All Rights Reserved.
Page 1/93
ORPHEE
19, chemin du champ-des-filles
CH-1228 Geneva / Plan-les-Ouates
SWITZERLAND
Tel : [phone]
Fax : [phone]
http://www.orphee-medical.com
Mythic™
Mythic™ 18 18 Vet Vet
Operator’s
Operator’s
Manual
Manual

REF : M18Vet/UM-EN/001
Page 2/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18

REF : M18 Vet/UM-EN/001
MYTHIC 18
Copyright© Orphée SA. All Rights Reserved.
Page 3/93
REVISIONS
Revision Nb
Date
Author
Software
Comments
01
01/02/2010
PhD
> V 1.0
Creation
CONTACT ADDRESS
MANUFACTURER
M
Manufactured in France for
ORPHEE SA
19, chemin du champ des filles
CH-1228 Geneva / Plan-les-Ouates
SWITZERLAND
Tel : [phone]
Fax : [phone]
http://www.orphee-medical.com
LOCAL AGENT

REF : M18Vet/UM-EN/001
Page 4/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18
READ THIS BEFORE USING THE EQUIPMENT
WARNING! RISK OF DANGER! Indicates a procedure to be strictly respected in
order to avoid any risks for the operator (user) or damages on the instrument or on
the quality of results.
Indicates that wearing gloves is mandatory before performing the described
operation due to risk of contact with materials that may be infectious.
NOTA
Indicates important additional information
DANGER
Misuse of electrical equipment may cause electrocution, burns, fire and other hazards.
Check that the voltage setting matches the supply voltage.
Protective earthing is required; plug the MYTHIC 18 VET into a supply outlet which has an earth connection.
Preserve a good access to the supply outlet to be able to unplug the MYTHIC 18 VET in emergency case.
Do not place the power supply adapter in liquid, nor put it where it could fall into liquid. If the power supply adapter
becomes wet, unplug it before touching it.
Do not use the MYTHIC 18 VET if it is not working properly, or if it has suffered any damage (damage to the supply
cord or its plug; damaged caused by dropping the power supply adapter).
Do not let the power supply adapter or its flexible cord come into contact with surfaces which are too hot to touch.
Do not place anything on top of the MYTHIC 18 VET
Do not use the MYTHIC 18 VET where aerosol sprays are being used, or where oxygen is being administered.
Do not use the MYTHIC 18 VET out of doors
Always switch off the MYTHIC 18 VET and disconnect the power adaptor before dismantling any part.
The MYTHIC 18 VET is an automated hematology analyzer for in vitro diagnostic use in clinical laboratories by an
authorized people.
- Only Veterinary blood or artificial control blood should be run.
- Only the reagents mentioned in this manual are permitted to use.
- The optimum performances can be only achieved if the cleaning and maintenance procedures are carefully
followed.
Due to the use of this equipment, all parts and surfaces of the MYTHIC 18 VET are potentially infective. Wearing
rubber gloves is highly recommended and after completion of work, washes hands with disinfectant.
Always replace or use parts of the equipment by parts supplied by ORPHEE distributor.
Basic safety precautions should always be taken. If the equipment is not used according to the manufacturer’s
instructions, the protective by the equipment may be impaired.
The treatment of waste and the elimination of a part or the complete instrument must be done in compliance with the
local legislation.
Any output or input connections (except the printer and the barcode reader supplied by ORPHEE) cannot be done
without the ORPHEE representative authorization.
Do not open the door located on the right side of the instrument (see section 1.1.3) when a hydraulic cycle is in
progress for it would lead to an immediate stop. To re-start, shut the door and run a Control cycle (see section 9.3.1)
KEEP THESE INSTRUCTIONS

REF : M18 Vet/UM-EN/001
MYTHIC 18
Copyright© Orphée SA. All Rights Reserved.
Page 5/93
This equipment needs special precautions regarding EMC and needs to be installed and put
into service according to the EMC information listed below.
Guidance and manufacturer’s declaration – Electromagnetic immunity
The MYTHIC 18 VET is intended for use in the electromagnetic environment specified below. The customer
or the user of the MYTHIC 18 VET should assure that it is used in such environment.
Immunity test
IEC 60601 test level
Compliance
level
Electromagnetic environment - guidance
Portable and mobile RF communications equipment should
be used no closer to any part of the MYTHIC 18 VET,
including cables, than the recommended separation
distance calculated from the equation applicable to the
frequency of the transmitter.
Recommended separation distance
Conducted RF
IEC 61000-4-6
3 Vrms
150Khz to 80Mhz
3 Vrms
d = 1,2√ P
Radiated RF
IEC 61000-4-3
3 Vrms
80Mhz to 2,5Ghz
3 Vrms
d = 1,2√ P 80MHz to 800MHz
d = 2,3√ P 800MHz to 2,5GHz
Where P is the maximum output power rating of the
transmitter in watts (W) according to the transmitter
manufacturer and d is the recommended separation
distance in meters (m).
Field strengths from fixed RF transmitters, as
determined by an electromagnetic site survey,ashould be
less than the compliance level in each frequency range
Interference may occur in the vicinity of equipment
marked with the following symbol:
NOTE 1 At 80Mhz and 800MHz, the higher frequency range applies.
NOTE 2 Theses guidelines may not apply in all situations. Electromagnetic propagation is affected by absorption and reflection from
structures, objects and people.
aField strengths from fixed transmitters, such as base stations for radio (cellular/cordless) telephones and land mobile radios,
amateur radio, AM an FM radio broadcast and TV broadcast cannot be predicted theoretically with accuracy. To assess the
electromagnetic environment due to fixed RF transmitters, an electromagnetic site survey should considered. If the measured field
strength in the location in which the MYTHIC 18 VET is used exceeds the applicable RF compliance level above, the MYTHIC 18 VET
should be observed to verify normal operation. If abnormal performance is observed, additional measures may be necessary, such as re-
orienting or relocating the MYTHIC 18 VET.
bOver the frequency range 150KHz to 80MHz, field strengths should be less than 3V/m.
The symbol on the product indicates that this product may not be treated as household waste. Instead it shall be handed over
the applicable collection point for the recycling of electrical and electronic equipment. By ensuring this product is disposed of
correctly, you will help prevent potential negative consequences for the environment and human health, which could otherwise be
caused by inappropriate waste handling of this product. For more detailed information about recycling of this product, please contact
your local city office or your distributor of this product.

REF : M18Vet/UM-EN/001
Page 6/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18
KONFORMITÄTSERKLÄRUNG /
DECLARATION DE CONFORMITE
DECLARATION OF CONFORMITY /
DICHIARAZIONE DI CONFORMITA
Name und Adresse der Firma
Nom et adresse de l’entreprise
Nome e indirizzo della ditta
Name and address of the firm
Orphée S.A.
19 Chemin du Champ des Filles
1228 Plan Les Ouates
Wir erklären in alleiniger Verantwortung, dass
Nous déclarons sous notre propre responsabilité que
Dichiariamo sotto nostra responsabilità che
We declare under our sole responsibility that
das Medizinprodukt für die In-vitro-Diagnostik
le dispositif médical de diagnostic in vitro
il dispositivo medico-diagnostico in vitro
the in vitro diagnostic medical device
Mythic 18 Vet
mit folgender Klassifizierung nach der Richtlinie über In-vitro-Diagnostika 98/79/EG
avec la classification selon la directive relative aux dispositifs médicaux de diagnostic in vitro 98/79/CE
con la classificazione secondo la direttiva relativa ai dispositivi medico-diagnostici in vitro 98/79/CE
classified as follows according to the directive on in vitro diagnostic medical devices 98/79/EC
Produkt der Liste A, Anhang II / Dispositif de la liste A, annexe II /
Dispositivo dell’elenco A, allegato II / Device of List A, Annex II
Produkt der Liste B, Anhang II / Dispositif de la liste B, annexe II /
Dispositivo dell’elenco B, allegato II / Device of List B, Annex II
Produkt zur Eigenanwendung, das nicht in Anhang II genannt ist /
Dispositif destiné à l’autodiagnostic non listé dans l’annexe II /
Dispositivo per test autodiagnostico non elencato nell’allegato II /
Device for self-testing not listed in Annex II
Sonstiges Produkt / Autre dispositif / Altro dispostivo / Other device
allen Anforderungen der Richtlinie über In-vitro-Diagnostika 98/79/EG entspricht, die anwendbar sind.
remplit toutes les exigences de la directive relative aux dispositifs médicaux de diagnostic in vitro 98/79/CE
qui le concernent.
soddisfa tutte le disposizioni della direttiva relativa ai dispositivi medico-diagnostici in vitro 98/79/CE che lo
riguardano.
meets all the provisions of the directive on in vitro diagnostic medical devices 98/79/EC which apply to it.

REF : M18 Vet/UM-EN/001
MYTHIC 18
Copyright© Orphée SA. All Rights Reserved.
Page 7/93
Angewandte Gemeinsame Technische
Spezifikationen, harmonisierte Normen,
nationale Normen oder andere normative
Dokumente
Spécifications techniques communes,
normes harmonisées, normes nationales et
autres documents normatifs appliqués
Specifiche tecniche comuni, norme
armonizzate o nazionali applicate, altri
documenti normativi applicati
Applied common technical specifications,
harmonised standards, national standards or
other normative documents
IEC 60601-1-2 (2001)
EN 61000-3-2
EN 61000-3-3
EN 61000-4-2 (95) A1 (98) A2 (01)
EN 61000-4-3 (02)
EN 61000-4-4 (95) A1(01)
EN 61000-4-5 A1 (01)
EN 61000-4-6 (96) A1 (01)
EN 61000-4-11 (94) A1 (01)
EN 55011 Class B
EN 55022 Class B
IEC 61010-1 (2001)
IEC 61010-2-081 (2001)
IEC 61010-2-101 (2002)
Konformitätsbewertungsverfahren
Procédure d’évaluation de la conformité
Procedimentodi valutazionedellaconformità
Conformity assessment procedure
Annex III
Konformitätsbewertungsstelle (falls beigezogen)
Organe respons. de l'évaluat.de la conformité(si
consulté)
Organo incaric. della valutaz. della conform. (se
consultato)
Notified Body (if consulted)
N/A
Ort, Datum / Lieu, date /
Luogo, data / Place, date
Genève le 06 Juin 2009
Name und Funktion / Nom et fonction /Nome e
funzione / Name and function
Philippe Daire
RA & QA

REF : M18Vet/UM-EN/001
Page 8/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18
TABLE OF CONTENTS
1. INSTALLATION 10
1.1 UNPACKING .........................................................................................................................................................................................10
1.1.1 Introduction..................................................................................................................................................................................................10
1.1.2 Unpacking Procedure ..................................................................................................................................................................................10
1.1.3 Visual checking..............................................................................................................................................................................................11
1.2 INSTALLATION CONSTRAINTS ..........................................................................................................................................................11
1.2.1 Installation place .........................................................................................................................................................................................11
1.2.2 Installation environment ...........................................................................................................................................................................11
1.3 ELECTRICAL CONNECTIONS ...............................................................................................................................................................12
1.3.1 Power supply block ......................................................................................................................................................................................12
1.4 PRINTER CONNECTION.......................................................................................................................................................................12
1.5 CONNECTION,CHANGE AND PRIMING REAGENTS .............................................................................................................................13
1.5.1 Connection.....................................................................................................................................................................................................13
1.5.2 Priming...........................................................................................................................................................................................................14
1.6 TRANSPORTATION AND STORAGE .....................................................................................................................................................16
2. GENERAL OVERVIEW 17
2.1 GENERALITIES ...................................................................................................................................................................................17
2.2 OVERVIEW .........................................................................................................................................................................................18
2.3 MAIN PART DESCRIPTION .................................................................................................................................................................19
2.3.1 Display / Keyboard .....................................................................................................................................................................................19
2.3.2 Dilution hydraulic part..............................................................................................................................................................................21
2.3.3 Mono electronic board .............................................................................................................................................................................22
2.3.4 Power Supply Block ...................................................................................................................................................................................22
2.3.5 Reagent tray...............................................................................................................................................................................................23
3. INSTRUMENT SET UP 24
3.1 USER’S IDENTIFICATION .................................................................................................................................................................24
3.1.1 Start Up........................................................................................................................................................................................................24
3.1.2 In process ....................................................................................................................................................................................................24
3.2 SYSTEM STATUS ...............................................................................................................................................................................25
3.3 SET UP ...............................................................................................................................................................................................26
3.4 ADVANCED SET-UP ............................................................................................................................................................................27
3.4.1 Printer set up:.............................................................................................................................................................................................27
3.4.2 Communication:...........................................................................................................................................................................................27
3.4.3 Analysis options: ........................................................................................................................................................................................28
3.4.4 Lab. parameters:........................................................................................................................................................................................28
3.4.5 Calibration factor: ....................................................................................................................................................................................32
3.4.6 Other Setting: ...........................................................................................................................................................................................32
3.4.7 Storage options: ........................................................................................................................................................................................33
3.4.8 Version release: .........................................................................................................................................................................................34
4. SPECIFICATIONS 35
4.1 ANALYTICAL SPECIFICATIONS.........................................................................................................................................................35
4.2 PHYSICAL SPECIFICATIONS .............................................................................................................................................................39
4.3 REAGENTS SPECIFICATIONS............................................................................................................................................................41
4.3.1 Diluent ...........................................................................................................................................................................................................41
4.3.2 Lysis reagent...............................................................................................................................................................................................41
4.3.3 Cleaning solution ........................................................................................................................................................................................42
4.4 ANALYTICAL LIMITATIONS..............................................................................................................................................................43
4.3.1 Recommendations.......................................................................................................................................................................................43
4.3.1 Interferences.............................................................................................................................................................................................43
5. SAMPLE ANALYSIS 44
5.1 VERIFICATIONS BEFORE STARTING .................................................................................................................................................44
5.2 START UP...........................................................................................................................................................................................44
5.3 REAGENT REPLACEMENT....................................................................................................................................................................45
5.4 START UP RINSING ...........................................................................................................................................................................46
5.5 PREPARATIONS BEFORE ANALYSIS...................................................................................................................................................46
5.6 ANALYSIS .........................................................................................................................................................................................46
5.6.1 Introduction................................................................................................................................................................................................46
5.6.2 Sample Identification ..............................................................................................................................................................................47
5.6.3 Sample run...................................................................................................................................................................................................49

REF : M18 Vet/UM-EN/001
MYTHIC 18
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5.7 RESULTS............................................................................................................................................................................................49
5.8 PRINTING...........................................................................................................................................................................................51
5.8.1 Model report (A4) – external printer....................................................................................................................................................51
5.8.2 Model report – Ticket printer ................................................................................................................................................................51
5.9 LOGS..................................................................................................................................................................................................52
5.10 ARCHIVE ..........................................................................................................................................................................................52
5.10.1 Results ........................................................................................................................................................................................................53
5.10.2 View.............................................................................................................................................................................................................54
5.11 STAND BY AND SHUT DOWN............................................................................................................................................................55
6. QUALITY CONTROL 56
6.1 INTRODUCTION .................................................................................................................................................................................56
6.2 QC.....................................................................................................................................................................................................56
6.2.1 Change...........................................................................................................................................................................................................58
6.2.2 Run control blood.......................................................................................................................................................................................59
6.2.3 Levey-Jennings graph...............................................................................................................................................................................60
6.2.4 Restore ........................................................................................................................................................................................................60
6.3 REPEATABILITY.................................................................................................................................................................................60
7. CALIBRATION 62
7.1 RESULTS.............................................................................................................................................................................................64
7.1.1 Calibration blood analysis .........................................................................................................................................................................64
7.1.2 Calibration....................................................................................................................................................................................................65
7.2 TARGET VALUE MODIFICATIONS ......................................................................................................................................................65
8. TECHNOLOGY 66
8.1 DETECTION PRINCIPLE ......................................................................................................................................................................66
8.1.1 WBC, RBC, PLT Counting ...........................................................................................................................................................................66
8.1.2 Hemoglobin measurement ........................................................................................................................................................................67
8.2 LEUCOCYTE ANALYSIS ......................................................................................................................................................................67
8.3 ERYTHROCYTE ANALYSIS..................................................................................................................................................................68
8.4 ANALYSIS OF PLATELETS .................................................................................................................................................................69
8.5 ALARMS .............................................................................................................................................................................................69
8.5.1 General Flags...............................................................................................................................................................................................69
8.5.2 Leukocytes Flags .......................................................................................................................................................................................70
8.5.3 Erythrocyte and HGB Flags ....................................................................................................................................................................70
8.5.4 Platelet Flags..............................................................................................................................................................................................70
8.5.5 Machine Flags.............................................................................................................................................................................................70
8.6 HYDRAULIC DESCRIPTION .................................................................................................................................................................71
8.6.1 Sampling module..........................................................................................................................................................................................71
8.6.2 Counting bath module................................................................................................................................................................................71
8.6.3 Syringes module .........................................................................................................................................................................................71
8.7 SOFTWARE ........................................................................................................................................................................................72
8.8.1 Windows .......................................................................................................................................................................................................72
9. SERVICE 73
9.1 MAINTENANCE ..................................................................................................................................................................................73
9.1.1 Maintenance table ......................................................................................................................................................................................73
9.1.2 Concentrate cleaning.................................................................................................................................................................................73
9.1.3 Piston greasing............................................................................................................................................................................................76
9.2 HYDRAULIC CYCLES ...........................................................................................................................................................................77
9.3 MECHANICS.......................................................................................................................................................................................78
9.4 REPAIRING ........................................................................................................................................................................................79
9.4.1 Emergency stop ..........................................................................................................................................................................................79
9.4.2 Needle or o-ring replacement ................................................................................................................................................................80
9.4.3 Baths dismantling ......................................................................................................................................................................................82
9.4.4 Baths o-ring replacement ........................................................................................................................................................................85
9.4.5 Aperture block replacement...................................................................................................................................................................85
9.5 TROUBLESHOOTING..........................................................................................................................................................................87
9.5.1 Analytical problems ...................................................................................................................................................................................87
9.5.2 Other problems..........................................................................................................................................................................................88
9.6 TROUBLESHOOTING MESSAGE .........................................................................................................................................................88
9.7 LOGS ERRORS .....................................................................................................................................................................................91
9.8 HYDRAULIC DIAGRAM .......................................................................................................................................................................92

REF : M18 Vet/UM-EN/001
1. INSTALLATION
MYTHIC 18
Copyright© Orphée SA. All Rights Reserved.
Page 10/93
1. INSTALLATION
1.1 UNPACKING
1.1.1 Introduction
The MYTHIC 18 VET is an automated hematology analyzer for in vitro diagnostic use in clinical laboratories
by an authorized people.
- Only Veterinary blood or artificial control blood should be run.
- Only the reagents mentioned in this manual are permitted to use.
- The optimum performances can be only achieved if the cleaning and maintenance procedures are
carefully followed.
If the MYTHIC 18 VET has been stored at a temperature less than 10°C, it must stay at
room temperature during 24 hours before switching it on.
1.1.2 Unpacking Procedure
Before unpacking the instrument, we recommend to check the box of the instrument and notify any damage
to the carrier.
-Open the box on the top, remove the starter kit.
-Remove the MYTHIC 18 VET from the box.
Starter kit contents:
INSTALLATION KIT
QTY
Designation
Part Number
1
M18 - WASTE
005-1001-90 24
1
70W switching adapter
050-1001-01 70 24
1
European Power line cord
150-2001-CEE C13 1000
1
M18 Vet User manual
605-1201-02
1
Screwdriver Slot 1/4"
700-7101-02
MAINTENANCE KIT
QTY
Designation
Part Number
1
Tygon tubing L=1000mm 1.52x3.2mm
005-1001-90 016 032
1
Tygon tubing L=500mm 2.06x4mm
005-1001-90 020 040
1
Tubing 9
005-1001-90 09
1
Tubing 10
005-1001-90 10
5
Cables Ties
153-0101-100 25
2
O-ring Ø13.1x1.6 Fluocarbon 80SH
312-0505-1310 160
1
O-ring Ø1.4x1.25 Fluocarbon 80SH
312-0505-140 125 010
2
O-ring Ø5x1 Fluocarbon 80SH
312-0505-500 100
1
Silicon grease (3gr)
410-0501-02 02
1
Short Arm TORX T10 Tool
700-1101-10
1
Short Arm TORX T20 Tool
700-1101-20

REF : M18 Vet/UM-EN/001
1. INSTALLATION
MYTHIC 18
Copyright© Orphée SA. All Rights Reserved.
Page 11/93
1.1.3 Visual checking
Open the door on the right side with the key
provided in the kit.
To be checked :
1- Counting chambers perfectly locked in their
manifold locations.
2- Needle’s dismountable system located in the
rocker.
3- Rocker in front position at the maximum course.
HAZARDOUS MOVING PARTS, BEWARE TO STAY AWAY FROM THESE PARTS WHEN THE
MACHINE IS SWITCH ON.
1.2 INSTALLATION CONSTRAINTS
1.2.1 Installation place
To ensure that the MYTHIC 18 VET fulfills its function, place the instrument on a table which supports the
weight of the instrument, printer and reagents (around 40 Kg). Leave a space of 10 cm in the rear of the
instrument to ensure a well-ventilated place. Avoid a place that can be exposed to direct sunlight.
1.2.2 Installation environment
a) Indoor use;
b) Altitude up to 2 000 m;
c) Temperature 18 °C to 32 °C;
d) Maximum relative humidity 80 % for temperatures up to 31 °C decreasing linearly to 50 % relative
humidity at 40 °C;
e) MAINS supply voltage fluctuations up to ±10 % of the nominal voltage;
f) Transient over voltages typically present on the MAINS supply.
g) Rated pollution degree II.
1
2
3

1. INSTALLATION
REF : M18 Vet/UM-EN/001
Page 12/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18
Please contact Orphée’s representative if you want to use the instrument in special conditions (height higher
than 2000 m or special power supply conditions).
1.3 ELECTRICAL CONNECTIONS
All the connectors are in the rear of the MYTHIC 18 VET
CONNECTION :
SYMBOL :
- 2 USB ports :
- Ethernet connection (TCP/IP) :
- External barcode reader (RS232C)
:
- Host connection (RS232C) :
- Printer connection (Centronics) :
- Power supply cord connection :
- Equipotentiality :
Any output or input connections (except the printer and the bar code reader supplied by
ORPHEE) cannot be done without the ORPHEE representative authorization.
1.3.1 Power supply block
MYTHIC 18 VET must be connected to the power with the power supply block provided with the starter kit.
Choose a well-ventilated place for the block and be sure to connect this power supply in a socket-outlet with
a correct earth connection.
The power supply block must be placed at the rear of the MYTHIC 18 VET and, if possible in an upper
position to avoid the contact with any liquid.
To disconnect electrically the MYTHIC 18 VET, remove the power supply plug from the main circuit.
- In the case of replacement of the main power wire supplied with the MYTHIC 18 VET the
new one must comply with the local regulation (31.5mm cable and 250V 10A plug).
- The MYTHIC 18 VET has been certified with the power supply box provided with the
machine. The use of another external power supply box is not guaranteed. Please contact
your Orphée’s representative.
1.4 PRINTER CONNECTION
Connect the printer cable in conformity with the printer user’s manual.
Use the parallel rear plug of the MYTHIC 18 VET ( )or the USB plug ( ) to connect the printer
cable. Select the printer driver (section 3.3).

REF : M18 Vet/UM-EN/001
1. INSTALLATION
MYTHIC 18
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Page 13/93
1.5 CONNECTION, CHANGE AND PRIMING REAGENTS
MYTHIC 18 VET works with the reagents described in section 4.3. Some commercial reagents can also be
used. Please contact your Orphée’s representative.
1.5.1 Connection
Pack installation:
Before handling the reagents, read carefully their
specifications described in section 4.3.
- Remove the door on the left side of the instrument.
- Put the M-Pack in the dedicated location.
- Remove the caps of the bottles
- Tighten the yellow caps on the Lysis bottle (yellow sticker), the
blue one on the cleaning solution bottle (blue sticker), and the
diluent cap on the bottle diluent.
Waste :
- Connect the waste tube (female connector) on the outlet on the
top and tighten the cap on an empty container.
Do not modify the type and the length of the
diluent and waste tubes.
The diluent must be placed at the same level as
the MYTHIC 18 VET.
Collect the waste in a container and treat it in
compliance with your local legislation.
Example of neutralization procedure usually used in a laboratory:
For 20 liters of waste produced by the MYTHIC 18 VET, add 50 ml of a solution of sodium hydroxide
(NaOH) at 200g/l, mix the container, add 100ml of bleach at 36° Cl, mix the container, wait one hour before
empty it.
Only use this procedure if there is no specific local legislation for the treatment of the
waste.
Waste

1. INSTALLATION
REF : M18 Vet/UM-EN/001
Page 14/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18
1.5.2 Priming
When first starting the MYTHIC 18 VET, it is necessary to perform a complete prime of the fluidic circuit.
Before starting, be sure that all the reagent and waste tubes are properly connected.
Priming procedure:
Switch on:
- Connect the power supply block.
- Press the ON/OFF button.
- The cycle LED turns red. No cycle can be performed before it turns
green.
- The information window could stay up to 3 mn to enable the update of all
files.
Login:
- The operator’s identification display appears.
- Enter the user’s identification, the password (see section 3.1) and press
to validate.
- AUTONOMY (run) indicates the number of samples (runs) you can perform
(calculated with the smaller quantity of reagents).

REF : M18 Vet/UM-EN/001
1. INSTALLATION
MYTHIC 18
Copyright© Orphée SA. All Rights Reserved.
Page 15/93
System priming:
- The main menu is displayed.
- If this window appears, it means that several
results in memory have not been sent before the
MYTHIC 18 VET was switched off.
- Press YES to send them immediately, or press
LATER to wait at another time or NO if you do
not want to send them.
- No USB key is available, connect an USB key
then press YES or see section 3.4.7 to change
the archive mode.
- Press on , then on REAGENTS to prime the pack.
- First, input LOT and Expiry date, press CHANGE RACK to validate.
- Second, press PRIME PACK to prime all the reagents.
-WASTE:
- Enter only the capacity of the container.
- After replacement of the waste container press
to reset to initialize the waste calculation.
MYTHIC 18 VET IS NOW READY TO WORK.

1. INSTALLATION
REF : M18 Vet/UM-EN/001
Page 16/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18
1.6 TRANSPORTATION AND STORAGE
Storage temperature: -10°C to +50°C.
Before transportation outside the laboratory, perform a complete cleaning with a disinfectant in compliance
with the local legislation.

2. GENERAL OVERVIEW
REF : M18 Vet/UM-EN/001
Page 17/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18
2. GENERAL OVERVIEW
2.1 GENERALITIES
MYTHIC 18 VET is a fully automated analyzer performing hematological analysis on whole blood collected
on EDTA tubes.
-Sample volume : 9,8 µl
-Throughput : 60 samples/hour
-18 analysis parameters :
Leukocyte parameters:
WBC White Blood Cells
LYM% Lymphocytes in percentage
LYM# Lymphocytes
MON% Monocytes in percentage
MON# Monocytes
GRA% Granulocytes in percentage
GRA# Granulocytes
Erythrocyte parameters
RBC Red Blood Cells
HGB Hemoglobin
HCT Hematocrit
MCV Mean Corpuscular Volume
MCH Mean Corpuscular Hemoglobin
MCHC Mean Corpuscular Hemoglobin Concentration
RDW Red Blood cells Distribution Width
Thrombocyte parameters
PLT Platelet
MPV Mean Platelet Volume
PDW* Platelet Distribution Width
PCT* Thrombocrit
* For Investigation Use only in the United States of America.

2. GENERAL OVERVIEW
REF : M18 Vet/UM-EN/001
Page 18/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18
2.2 OVERVIEW
MYTHIC 18 VET consist of 8 main parts:
1. Display / Keyboard.
2. Dilution hydraulic part.
3. Mono electronic board.
4. Reagent tray.
5. Connection.
6. External power supply block.
7. Printer.
8. Barcode reader (option).

REF : M18 Vet/UM-EN/001
2. GENERAL OVERVIEW
MYTHIC 18
Copyright© Orphée SA. All Rights Reserved.
Page 19/93
2.3 MAIN PART DESCRIPTION
2.3.1 Display / Keyboard
Stand By and
ON/OFF button
Et Marche/Arrêt
Numeric keyboard
Delete (DEL)
Exit (ESCAPE)
Cursors
Enter (ENTER)
Cycle in process
Led
Touch screen LCD
display 380*240

2. GENERAL OVERVIEW
REF : M18 Vet/UM-EN/001
Page 20/93
Copyright© Orphée SA. All Rights Reserved.
MYTHIC 18
Main Menu description
Back to previous
screen
Print,
Send,
Select
Options
Direct access to
main menu
Start Up rinsing
and Blank control
Cleaning and stand by
mode
Date and Time.
System status
(section 3.2).
Archive of results
QC and Calibration menu
Access to:
- Logs
- Set Up
- Reagents
- Service
Run Samples :
-Cat
-Dog
-Horse
-Others species