PERSEE T6 U User manual

PERSEE ANALYTICS, INC.
T6 U
Spectrophotometer
INSTRUCTION MANUAL

i
Introduction
Thanks for your purchase of the PERSEEANALYTICS, INC. T6U spectrophotometer.
UV-Visible Spectrophotometer is a well-accepted, documented technique with many applications. The
technique is extensively used for the analysis of agricultural products and is widely used in public health,
environmental protection, life sciences industries and many other organic and biochemical applications.
PERSEE ANALYTICS, INC. developed its latest spectrophotometer-T6U based on its proved
technology. The advanced mould design ensures the outstanding quality, excellent specifications and stable
performance, which can fully meet the requirements of the analyzer.
For obtaining maximum performance and trouble-free operation, we give you the following
suggestions:
◆Upon receiving your instrument, please check all the items listed in the packing list are
included and install the instrument according to the instruction manual.
◆The spectrophotometer is a precise electronic instrument. Please read the instruction manual
carefully before operation. If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be impaired.
◆Avoid collision and handle the instrument carefully during transportation.
◆If the tested solution is harmful to humans, please wear gloves or use other protection
methods.
◆Please keep the accessories in a safe place for the use in future maintenance.
For more information, please visit www.perseena.com

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Icons
Please pay attention to the following icons appearing in the book.
and Direction guide: direction guide when you mount and dismount the instrument.
Caution: Improper operation may cause unnecessary damage or accidents.
Eyes protection: Please wear glasses for the inner ultraviolet radiation and strong light.
Scald: Don’t touch to avoid scalding.
Wear gloves: Please wear gloves when you maintain some parts.
Don’t touch: Do not touch the optics components.

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Contents
CHAPTER 1 INSTALLATION.................................................................................................................... 1
1.1 Packing List........................................................................................................................ 1
1.2 Environmental Requirements.............................................................................. 2
1.3 check the mains voltage.......................................................................................... 3
1.4 Instrument Installation............................................................................................... 4
CHAPTER 2 INSTRUMENT CONSTRUCTION ..................................................................................... 6
2.1 Front view............................................................................................................................. 6
2.2 Side view............................................................................................................................... 6
2.3 Rear view............................................................................................................................... 7
2.4 keypad description....................................................................................................... 7
CHAPTER 3 OPERATION......................................................................................................................... 8
3.1 Introduction......................................................................................................................... 8
3.2 Photometric Measurement..................................................................................... 9
3.3 Card Program.................................................................................................................. 15
3.4 System Set......................................................................................................................... 16
CHAPTER 4 SPECIFICATIONS.............................................................................................................. 21
CHAPTER 5 OPTIONAL ACCESSORIES............................................................................................ 22
CHAPTER 6 MAINTENANCE................................................................................................................. 23
6.1 Daily Care............................................................................................................................ 23
6.2 Maintenance..................................................................................................................... 24
APPENDIX 1............................................................................................................................................... 26
APPENDIX 2............................................................................................................................................... 28

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Announcement
This manual contains copyrighted and proprietary information. No part of this publication may be
reproduced, or transmitted in any form by any means, electronic, mechanical, photocopying, recording or
otherwise, without the permission of PERSEE ANALYTICS, INC.
Information in this publication is subject to change without notice, as PERSEE ANALYTICS, INC.
continues to develop the latest technologies and products for our customers.
The units described in this manual are designed to be operated only by professionals.
Any errors or omissions that may have occurred in this publication despite the utmost care taken in its
production will be corrected as soon as possible, but not necessarily upon detection.

1
Chapter 1 Installation
1.1 Packing List
T6U main unit and the necessary accessories are packed in the packing box. We also provide optional
accessories at your discretion. Upon receiving your instrument, please check that all the items listed below
are present:
NO.
Name
Q’ty
01
T6U main unit
1
02
Fuse (T, 250V, 2A)
2
03
Quantitative program card
1
04
Installation guide
1
05
Black block
1
06
AC power cord
1
07
Instruction manual
2
08
Packing list
1
09
Quartz cell (10mm)
2
10
Tool kit
1
Table 1-1
Any shortage or damage should be reported immediately to your local dealer.
Please keep the packing and accessories for the possible later packing
transportation and maintenance.

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1.2 Environmental Requirements
To insure correct operation and stable performance over an extended period of time, install the T6U in
a location that meets the following conditions:
1. Operation conditions:
Condition
Altitude
Temp (ºC)
Humidity (%RH)
Non-condensing
Non-operating
(Transport)
0-2000m
5-45
20-80
Non-operating
&meeting
dielectric
strength tests
Sea level
40
91-95
Operating but
not
necessarily
meeting
performance
specifications
0-2000m
5-31
31-40
≤80
≤[80-3.33(t-31)]
Operating
within
performance
specifications
0-853m
853-2000m
10-35
10-25
8-80
8-80
2. Protection degree: IP20
3. Avoid magnetic fields
Away from the electric appliances with magnetic field, electromagnetic field and high frequency
waves;
4. Avoid corrosive gas
Area free from corrosive gas, such as chlorine, hydrochloric acid gas, hydrogen sulphides gas,
sulfurous acid gas;
5. Area with very little dust or other airborne particles.
6. Placing on the stable workbench
The workbench should be stable and strong enough to support the total weight of equipment to be used.
The worktops should be large enough to permit a free circulation of air around each unit in the system.
Remember to provide space for the computer, monitor and printer. The workbench should be about 90cm
high. To avoid damage through spillage of the samples being analyzed, the worktops should be covered
with a material that is corrosion resistant and impervious to liquids.
7. Do not share a power socket with other electric appliances.
Equip the instrument with a power socket, which should not be shared with other electric appliance.
The power should be with protective ground wire.
8. Avoid direct sunlight
The improper environment will affect the measurement result and the
instrument service life!

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1.3 check the mains voltage
Verify the mains voltage before turning on the power, or it may damage the instrument.
T6U is designed to operate on 230V, 120VA and 50/60Hz mains supply.
Confirm the AC main is earthed in the building before turning on the instrument.
Turn off the power and cover the instrument after use.

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1.4 Instrument Installation
The following procedures will help you finish the installation.
Step one: Unpack the instrument and place it on the stable workbench.
Unpack the instrument carefully and set it down gently! The workbench should be stable and
free from vibration.
Step two: Connect to the printer
Be sure to turn off the power of the instrument and the printer before connecting via the parallel
port, otherwise it may damage the devices.
Step three: Connect the power cords, and then put the plug into the AC outlet in the building.
Confirm that the power switch is set to “0”before connecting the power cords.
Step four: Switch on the instrument power and then it will start initialization, as shown in the following
display.

5
Step five: When the initialization procedure is completed, the main menu will automatically appear on the
screen.

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Chapter 2 Instrument construction
2.1 Front view
2.2 Side view

7
2.3 Rear view
2.4 keypad description
Function keys
Set the working mode
Set the wavelength
Zero Abs (100%T)
Print data
Edit keys
Start measurement
Used to quit operation and
return
Used to confirm the current
parameters and operating mode
Numeric keys
Input the numeric values
Page keys
Page up
Page down

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Chapter 3 Operation
3.1 Introduction
The main menu
(Fig.3-1)
Press 【▲】 and 【▼】 to move the cursor to the required function, then press【ENTER】to gain
access to the screen of selected option; Press 【RETURN】to return to the preceding page.
There are three options available on the main menu:
⊙Photometric Measurement
This mode allows the user to measure Abs or transmittance at a fixed wavelength and K factor and
print out.
⊙Card Program
The instrument can perform a required measurement such as quantitative measurement, DNA/Protein
analysis etc. by loading a pre-programmed card into the extended interface of the instrument. If there is no
pre-programmed card in the extended interface, this option is inoperative.
⊙System Set
This mode allows the user to specify the working parameters, such as backlight working mode,
tungsten lamp On/Off, deuterium lamp On/Off, control mode, time set, wavelength calibration, and dark
current correction etc.

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3.2 Photometric Measurement
This mode allows the user to measure the Abs or transmittance at a fixed wavelength, and perform
measurement by using K factor; you also can print out the measurement result.
Main menu of photometric measurement
Press【▲】 and 【▼】 to select “Photo”, then press【ENTER】to gain access to the main menu of
photometric measurement.
(Fig.3-2)
Setting parameters
In the case of photometric measurement (Fig.3-2), press【SET】to gain access to the menu of
parameters setting.
(Fig.3-3)
Press 【▲】 and 【▼】 to move the cursor to the required option, then press 【ENTER】to gain
access to the required menu. Press 【RETURN】to quit, returning to the preceding menu.
Selecting the photometric method
On the menu of (Fig.3-3), select ⊙Method, then press 【ENTER】to display the menu as shown
in (Fig.3-4):
(Fig.3-4)
Press 【▲】 and 【▼】 to move the cursor to the required option, then press【ENTER】to gain
access to the relevant menu. Press 【RETURN】to quit, returning to the preceding menu.
When the user selects energy, the gain setting menu will be displayed (Fig.3-5):

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(Fig.3-5)
At the bottom of this page, enter the gain value by pressing the numeric keys (1~8). The range is 1~8.
If the entered figure exceeds the range, the system will delete what has been entered automatically and you
should start a new entry again. Press 【ENTER】to confirm, and press 【RETURN】to quit, returning to
the menu of parameters setting.
Note: Generally speaking, the user needn’t use the energy method.
Selecting math calculation
On the menu of (Fig.3-3), select ⊙Math Count, then press 【ENTER】to display the menu as
shown in (Fig.3-6):
(Fig.3-6)
At the bottom of this page, enter the K factor by pressing the numeric keys (0~9), decimal point【．】
and negative sign 【-】. Press 【RETURN】key to clear the mistakes, and press 【CE】to clear step
by step, then press 【ENTER】key to confirm. The system will prompt the user to input factor B. Press
【ENTER】key to confirm and quit. If nothing is to be entered, press【RETURN】key to quit this page
directly. The range is from -999 to 999. If the entered figure exceeds the range, the system will delete what
has been entered automatically and you should start a new entry again.
Selecting the sample cell
On the menu of (Fig.3-3), select ⊙Cell Setting, then press 【ENTER】to display the menu as
shown in (Fig.3-7) (Fig.3-8):

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(Fig.3-7)
(Fig.3-8)
Press 【▲】 and 【▼】 to move the cursor to the required option, then press【ENTER】to gain
access to the menu. Press 【RETURN】to quit, returning to the preceding menu.
⊙Setting Module Style
Press 【▲】 and 【▼】 to move the cursor to the required option, then press【ENTER】to select
among “8 cell”, “5 cell”and “fixed cell”. If the user choose “fixed cell”, the options irrelated to the fixed
cell are inoperative.
⊙Cell Number
This option allows the user set the cells in use. Press 【▲】 and 【▼】 to move the cursor to the
required option, then press【ENTER】. The figure will increases one by pressing 【ENTER】each time.
The range is 1—8.
⊙Reagent Blank/Cell Blank/Blank Test
1) Reagent Blank
Press 【▲】 and 【▼】 to move the cursor to the required option, then press【ENTER】to toggle
between “yes”and “no”. If you choose “yes”, the system will take the sample in the No.1 Cell as the
reagent blank to measure the samples in other cells (2-8).
2) Cell Blank
Press 【▲】 and 【▼】 to move the cursor to the required option, then press【ENTER】to toggle
between “yes”and “no”. The sample cells will differ from each other in optics characteristics subtly even
though they are made in the same way. When the user start cell blank, first put the blank in each sample
cell and save the measured value. Then put and measure the unknown sample in the cells. Using the
measured value deducts the saved blank sample value, and you can get the required measurement result.
3) Blank Test
Press 【▲】 and 【▼】 to move the cursor to the required option, then press【ENTER】to test the

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blank value. If you select yes for the cell blank, the test is necessary. Put blank samples in each cell before
test, the system will record the measurement values of each cell automatically.
If you select “yes”for both reagent blank and cell blank, you can perform cell blank for each cell and
take the sample in No.1 cell as the blank to correct the measured values of other cells.
Blank correction is a kind of data processing. The following table shows the calculation.
Cell
number
Reagent blank and
Cell blank are
“NO”
Only the
reagent
blank
“YES”
Only the
cell blank
“YES”
Both blank
correction
“YES”
The
output
data
NO. n
sample
cell
(n=2---8)
Abs
An
An - a1
An - an
(An - an) -
( A1 - a1)
Transmittance
Tn
Tn / t1 ×
100(%)
Tn / tn ×
100(%)
Tn * t1 / tn *
T1 ×
100(%)
NO. 1
sample
cell (as
the
reagent
sample
cell)
Abs
A1
A1
A1 - a1
A1 - a1
Transmittance
T1
T1
T1 / t1 ×
100(%)
T1 / t1 ×
100(%)
An
The Abs of NO. n sample cell without
correction
Tn
The transmittance of NO. n sample cell
without correction
an
The blank value of NO. n sample cell (Abs)
tn
The blank value of NO. n sample cell
(transmittance)
⊙Move Cell
Press【▲】and【▼】to move the cursor to the required option, then press【ENTER】. The sample
cell will move forward one cell by pressing【ENTER】each time. When it moves to the maximum cell
number, next it will return to No.1 cell automatically. If the user selects fixed cell, the option is
inoperative.
⊙Factor K
Input the required magnified multiple times of the measurement value.
Press 【▲】 and 【▼】 to move the cursor to the required option, then press 【ENTER】to set
factor K. At the bottom of this page, enter the factor K by pressing the numeric keys (0~9), decimal point
【．】and negative sign【-】. Press【RETURN】key to clear the mistakes, and press【CE】to clear step
by step, then press【ENTER】key to confirm . If nothing is to be entered, press 【RETURN】key to quit
this page directly. The range is from -99 to 99. If the entered figure exceeds the range, the system will
delete what has been entered automatically and you should start a new entry again.
⊙Cell Init.
If the user moves the cell during measurement, cell init. should be performed.
Press【▲】and【▼】to move the cursor to the required option, then press【ENTER】to perform
cell init. It will return to No.1 cell.

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Setting the working wavelength
In the case of photometric measurement (Fig.3-1), press【GOTOλ】to set wavelength, as shown in
Fig.3-9.
(Fig. 3-9)
At the bottom of this page, enter the wavelength by pressing the numeric keys (0~9) and decimal point
【．】. Press 【RETURN】key to clear the mistakes, and press 【CE】to clear step by step, then press
【ENTER】key to confirm . If nothing is to be entered, press 【RETURN】key to quit this page
directly. The range is from 190 to 1100. If the entered figure exceeds the range, the system will delete what
has been entered automatically and you should start a new entry again.
Auto Zero
In the case of photometric measurement (Fig.3-1), press 【ZERO】to zero the Abs (or 100%T) at
current wavelength. Put the blank sample into the sample compartment before correction.
Measurement
In the case of photometric measurement (Fig.3-1), press 【START】to gain access to the measurement
menu, then press 【START】again to measure the samples at current wavelength in the auto cell changer
or the fixed cell holder, as shown in Fig.3-10.
(Fig. 3-10)
500.0 nm
The current wavelength
1.000Abs
The actual measured value of current cell
NO.1-1
The first measurement value of No.1 Cell
Abs
Current measurement mode
K*Abs
Factor K multiply by the current Abs
Five rows of data can be displayed on the screen, the others can be displayed by pressing 【▲】 and
【▼】.
Printout

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On the menu of Fig.3-10, press 【PRINT】to print the data in the data area. The system will clear the
data area after printout.
When the data area is full or the user changes the setting of the system, it will ask the user whether to
print the measured data when the user starting measurement the next time. The user can select print or not.
If the user chooses “Print”, the system will print the measured data. If the user chooses “Don’t print”, the
system will clear the data area.
Changing the system setting means changing the measurement mode or wavelength after getting data
in the measurement mode, such as changing from photometric measurement toAbs measurement.
Note: the data area can save 64 groups of data at most, please printout your data in time.

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3.3 Card Program
The MCU mode only allows the user to perform photometric measurement. If you want to perform
other measurements, please insert the required program card into the instrument.
We provide multiple program cards for your option, as well as other cell holders and software etc.
Please refer to the optional accessories for the detailed information of accessories.
Loading the program card
Make sure the instrument power is off. Insert the program card into the extended interface. Turn on the
instrument and perform initialization. Then, press【▲】 and 【▼】 to move the cursor to the option of ⊙
Card Program on the menu of Fig.3-11. And then press 【ENTER】to gain access to the required menu.
Refer to the instruction manual of the program card for the detailed information.
(Fig. 3-11)
Unloading the program card
Please turn off the instrument before taking out the card. Press the pushing bar above the slot to take
out the card.
Inputting the serial number
When the user uses the program card for the first time, the system will ask the user input the serial
number, which was given to the user by the manufactory when he bought the cards. The user also can
acquire the serial number by telephone. Please keep the serial number in an appropriate place.
If the user input the wrong number for more than 5 times, the system will return to the main menu.
The user should make sure the accuracy of the serial number, if not, please contact our service personnel.
The user is required to input the serial number when he uses the card for the first time, and needn’t to input
afterwards.
Do not insert or take out your program card when power on. Please keep your program card
appropriately.
The program card can only be inserted into the left extended slot, as shown in Fig. 3-11.