PERSEE PF7 User manual

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Instruction manual
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Contents
Chapter 1 Preface...................................................................................................... 1
1.1 Purpose............................................................................................................ 1
1.2 Principle............................................................................................................ 1
1.3 Structure........................................................................................................... 2
1.4 Main Features .................................................................................................. 3
1.4.1 Special pneumatic flow system .............................................................. 3
1.4.2 Double beam optical system.................................................................. 3
1.4.3 New Design gas-liquid separator ........................................................... 3
1.4.4 Efficient electronic Peltier....................................................................... 3
1.4.5 Pre-aligned lamp assemblies................................................................. 3
1.4.6 Scientific structural design...................................................................... 4
1.5 Specifications................................................................................................... 4
1.5.1 Performance........................................................................................... 4
1.5.2 Key Parts List......................................................................................... 4
1.5.3 Optional Accessories.............................................................................. 5
1.5.4 Optional Accessory Information.............................................................. 5
Chapter 2 Installation and Acceptance.......................................................................... 6
2.1 Lab requirements ............................................................................................. 6
2.1.1 Lab environment conditions: .................................................................. 6
2.1.2 Equipment requirements for the lab....................................................... 6
2.1.3 Gas Source Requirements..................................................................... 7
2.1.4 Power Supply Requirements.................................................................. 7
2.3 Installation........................................................................................................ 8
2.3.1. Connecting the computer system：...................................................... 8
2.3.2. Connecting with the PC system. ........................................................... 9
2.3.3 PF7 installation....................................................................................... 9
2.4 Acceptance......................................................................................................11
2.5 Testing method................................................................................................12
2.5.1 Test Conditions......................................................................................12
2.5.2 Repeatability (Relative Standard Deviation /RSD）..............................12
2.5.3 Detection Limit (DL) and Correlation Coefficient (R) .............................13
Chapter 3 Software Installation ....................................................................................14
3.1 PC Configuration.............................................................................................14
3.2 Installation.......................................................................................................14
3.3 The File System ..............................................................................................15
Chapter 4 AFWin Software Introduction .......................................................................16
4.1 Login ...............................................................................................................16
4.2 Initialization......................................................................................................16
4.3 Main Operating Interface.................................................................................17

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Chapter 5 AFWin Functions..........................................................................................18
5.1 Parameter settings..........................................................................................18
5.1.1 Instrument Control.................................................................................18
5.1.2 Sampling and measurement settings....................................................20
5.2.2 Standard Sample Concentration ...........................................................21
5.2.3 Sample Settings....................................................................................24
5.2 Sample Measurement .....................................................................................28
5.3.1 Measurement........................................................................................28
5.2.2 Save result............................................................................................29
5.2.3 Print result.............................................................................................29
5.3.4 Print preview .........................................................................................30
5.3.5 Right key menu in chart.........................................................................30
5.3 Calibration Curve.............................................................................................31
5.4 Testing result...................................................................................................32
5.5 User management...........................................................................................34
5.6 Set communication port...................................................................................35
5.7 Scanning .........................................................................................................35
Chapter 6 Analysis Conditions......................................................................................37
6.1 Arsenic (As).....................................................................................................37
6.2 Antimony (Sb)..................................................................................................38
6.3 Bismuth (Bi).....................................................................................................39
6.4 Mercury (Hg) ...................................................................................................41
6.5 Selenium (Se)..................................................................................................42
6.6 Tellurium (Te)...................................................................................................43
6.7 Tin (Sn)............................................................................................................45
6.8 Germanium (Ge) .............................................................................................46
6.9 Lead (Pb).........................................................................................................47
6.10 Zinc (Zn)........................................................................................................48
6.11 Cadmium (Cd)...............................................................................................49
Chapter 7 Notice of Storage andTransportation...........................................................51
Chapter 8 Daily Care and Maintenance........................................................................52
8.1 Daily care ........................................................................................................52
8.2 Troubleshooting...............................................................................................52
8.2.1 Tools......................................................................................................52
8.2.2 Communication failed............................................................................52
8.2.3 The problem of software........................................................................53
8.2.4 The problem of element lamp................................................................54
8.2.5 The problem of the testing.....................................................................54
8.2.6 Injection needle error of auto-sampler...................................................55
8.2.7 PC.........................................................................................................56
Appendix 1 Replacement of furnacefilament................................................................57
Appendix 2 After sales service .....................................................................................58

Instruction manual
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Chapter 1 Preface
The Atomic Fluorescence Spectrophotometer is an excellent element analysis instrument
whichhas the advantages of atomic absorption spectrophotometer and atomic emission
spectrophotometer but overcomes their shortages in techniques. The instrument has good
accuracy, high sensitivity, simple structure, small volume and easy operation. It is designed
to test elements that could easily form hydride, gaseous constituents or deoxidizes to atomic
smoke. It is dedicated to the analysis of Arsenic(As), Mercury(Hg), Cadmium(Cd),
Germanium(Ge), Selenium(Se), Lead(Pb), Bismuth(Bi), Tellurium(Ti), Tin(Sn), Zinc(Zn),
Antimony(Sb) elements.
1.1 Purpose
The Atomic Fluorescence Spectrophotometer can be widely used in many industries, such
asenvironmental, food, pharmaceutics, water supply and drainage, cosmetics, soil and
fertilizer,clinical, agricultural products, geologic investigation, metallurgy, teaching and
research.
1.2 Principle
Principle: as shown in figure 1-1, The Gas source provides a constant pressure environment
for a constant liquid supply and solenoid valve control. The gas pressure takes up the
sample (in sample loop) and the KBH4 (in reaction bottle) into the gas liquid separator which
reacts and produces hydride gas (or atomic vapor). Hydride gas enters the atomizer and is
atomized, producing fluorescence light. The detector receives and tests intensity of the
fluorescence light which then transmits a signal to the processor. The processed result is
seen at the software on the PC system.
The instrument measures characteristics of the radiation exposure in the measured
elements of atomic vapor, around the electronic ground state atoms that are excited to be
high-energy state. Because the high-energy electronic state of the atoms become unstable,
they return to a low energy state, The external radiation is measured by Fluorescence which
is used for the quantitative analysis. The fluorescence intensity and the concentration of
elements following the relations: In the formula：
If=ΦI0(I-e-KλLN)
If ---------atomic fluorescence intensity；
Φ---------atomic fluorescence quantum efficiency；
I0---------Source radiation intense
Kλ--------Peak absorption in wavelengthλ；
L----------Light-absorption；

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N---------Units within the length of the ground state atoms
Fig. 1.1 Principle of PF7
1.3 Structure
The PF7 basic instrument information is shown in figure 1-2：
Figure 1-2 Sketch photo of PF7

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1.4 Main Features
1.4.1 Special pneumatic flow system
Argon is used as a driving source to achieve the automatic sampling, quantitative, discharge
functions with no need for maintenance. The pneumatic flow system is used instead of a
sipper pump system to avoid ripple caused by sipper pump and sampling errors caused by
tube deformation. The material of the carrier tube and reducer tube is FEP which has
excellent chemical stability and has a much longer life. The pneumatic sequential injection
system is used for the auto dilution, on-line washing of the sampling system which avoids
sample residue and high-concentration samples contamination. This helps to guarantee the
reliability of analytical measurement. The fully enclosed reagent bottles reduce the
decomposition rate of the reducer and guarantees the stability of long-term measurements.
1.4.2 Double beam optical system
The unique double beam optical design achieves time-share sample signal and reference
signal collection by one detector, and overcomes the influence on the results by the
temperature drift of the detector in a single beam instrument and has a significant correction
for lamp drift.
1.4.3 New Design gas-liquid separator
A magnetic stirring hydride generator changes the way the reaction is produced of existing
atomic fluorescence injection systems. The simplified flow structure of the atomic
fluorescence has the sample and reducer solutions inflow directly into the gas-liquid
separator via a three-way valve. The magnetic stirring forms an increased plume, thus
making the sample and reducer mixing more complete, and the reaction more intense. This
effectively improves the efficiency of the hydride’s outflow, transmission and reproducibility
of the analytical results.
1.4.4 Efficient electronic Peltier
The Peltier is used as a condensing device and offers direct contact cooling for the
gas-liquid separator, minimizing the water content of the hydride. This greatly reduces the
signal scattering interference and quenching which improves the detection sensitivity,
1.4.5 Pre-aligned lamp assemblies
The well-designed integrated pre-aligned element lamp with built-in electrical cable offers
automatic recognition, hot plug functionality and plug and play. The lamps are pre-aligned to
give consistency of the beam.

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1.4.6 Scientific structural design
Harmful gas exhaust is emitted via the exhaust hood. Due to the sealed design the
emissions are vastly reduced. The relatively high density harmful elements are absorbed by
a special reagent bag minimizing harmful gas inhalation. A fully enclosed waste bottle avoids
volatile of acid and waste gas, reducing environmental pollution and laboratory personnel
injuries. Inward optical design avoids ultraviolet light damage to personnel.
1.5 Specifications
1.5.1 Performance
The instrument should be allowed to warm up for > 30 minutes
Table 1.1 Specifications of PF7
Performance
specification
Stability
Drift ≤1.5%
Noise ≤1.5%
Reproducibility (RSD)
≤1.0%
Detection Limit (D.L.)
≤ 0.01 ng/mL ( Hg ≤ 0.001ng/ml )
Correlation coefficient (r)
≥ 0.999
Channel interference
±2%
Instrument
parameters
Optical system
Double beam
Communication interface
RS232, CAN
PC system
WINDOWSXP，WIN7，WIN8
Power
AC(100～240)V，50/60Hz，300W
All specifications above for As, Sb, Bi
Detection limit for Hg ≤0.001ng/ml
1.5.2 Key Parts List
Table 1-2
No.
Part No.
Name
1
A1701-02-01-03-00DQ
As lamp assembly
2
A1701-02-01-13-00DQ
Sb lamp assembly
3
A1701-02-01-09-00DQ
Bi lamp assembly
4
A1701-02-01-04-00DQ
Hg lamp assembly
5
A1701-06-05-00DQ
Main board
6
DH0200715401
PMT R7145

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Table 1-3
No.
Part No.
Name
1
A1701-04-10
Gas-liquid separator
2
S1201-04-01-00
Sampling tube assembly
3
9601-02-08-02-09
Resistance wire
4
9601-29-02-07
Target
5
A1701-13-00
Reagent bottle assembly
6
9601-02-08-02-15
Quartz tube
7
DK 1225330501
Fuse (5×20-3A)
8
A1701-29-02-01-01-00
As lamp package
9
A1701-29-02-01-10-00
Sb lamp package
10
A1701-29-02-01-11-00
Bi lamp package
11
A1701-29-02-01-02-00
Hg lamp package
12
A1701-14-02
Three Way Mixer
13
0987-05-000
987 Adapter
14
A1701-14-15-00
Waste bottle
1.5.3 Optional Accessories
Table 1-4
No.
Name
1
Speciation analysis accessories SA420 (isocratic orgradient)
2
AS420 Auto-sampler
3
WTM7 trace Hg in water analysis accessory
4
TGU7 Exhaust treatment accessory
5
MIU7 Micro-volume Syringe accessory
6
Fingerprint identification
7
Humidor Meter accessory
8
Laser Bar Code Reader
9
Bar code printer
1.5.4 Optional Accessory Information
Please contact your local dealer or Persee Analytics Inc. directly for information about our
optional accessories.

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Chapter 2 Installation and Acceptance
2.1 Lab requirements
2.1.1 Lab environment conditions:
The lab should be located away from strong electromagnetic interferences, strong light
source,strong vibrating equipment. The instrument should also be kept from influences of
direct sunshine,smoke dust, dirty air current and vapor. The AFS lab should be separated
from any chemical treatment room so as to avoid acid erosion. The lab should always be
kept clean with a correct temperature (at 15-35°C) and humidity (below 75% relative
humidity).
2.1.2 Equipment requirements for the lab
The laboratory bench should be large enough to install the PF7 instrument, Auto
Sampler and Speciation Accessory (if required), at a recommended height of 0.75 meters; it
should be stable and solid with a flat surface.
Around the bench, a space at least half a meter should be left to facilitate the operation
and maintenance.
The bench and instrument should be positioned out of direct sunlight, close to electricity
and gas connections.
Size and Weights of Instruments and Accessories:
Instrument
Length
Width
Height
Weight
AF402
610mm
595mm
450mm
35Kg
Auto Sampler
420mm
320mm
320mm
20Kg
SpeciationAccessory
600mm
560mm
415mm
40Kg
Efficient fume extraction should be installed in the laboratory to remove any fumes
generated by the instrument. See figure 2.1.
Note: Without adequate extraction, potentially toxic vapour could build up in the
laboratory. Always use an efficient fume extraction system when operating the instrument.

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A suitable extraction system should be fitted as Figure 2-1 above
2.1.3 Gas Source Requirements
It is the responsibility of the user to ensure that the gas installation conforms to the local
safety regulations.
The Argon gas, when configured with the AFS system, should have a purity of 99.99%.
Thesupply pressure should be set using a gas regulator. Aspecial gas cylinder storage room
is needed and a gas on-off valve in the laboratory should be installed.
The gas pressure should be set to 40psi / 2.8bar
2.1.4 Power Supply Requirements
The instrument and its accessories use a 220v AC (+/-10%) 50/60Hz power supply with
standard mains plugs, so a standard switchboard arrangement can be used. The power
supply should be free from noise and have a good earth connection. If the supply is outside
the requirements, a stabilized power supply unit can be used but must be rated higher than
1.5KW.
Please note: The main unit of the AFS, Auto Sampler and Accessories should all be
connected to the same electrical phase
Preparations before installation
1. Items check: unpack the instrument; check item by item against the packing list to
see if the main unit, accessories, parts, spare parts and the operation manual are
complete. Please check if there is any damage to the appearance of the instrument.
If there is a problem, please contact your local distributor.
2. Please read the operation manual carefully to familiarize yourself with the operating
principles and structure of the instrument.
3. Prepare the power supply, the fuel gas tubing, the waste fluid collector and the