PIP 120260 User manual
+98 2188765561
ACCREDITED®
Management
systems
Certification Body
MSCB 105
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Institute of Global Certification
CERTIFIED
ISO13485:2016
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Institute of Global Certification
CERTIFIED
ISO 9001:2015
+98 912 3340197
www.medpip.com
Pole Ideal Pars Co.
poleideal
Single Channel
Pipettes
National Health, Not Only Our Job
But Also, Our Mission
Innovation, Precision, Quality
Pole Ideal Pars )P.I.P.( is a manufacturing company, providing products that
are designed to promote innovation and improve productivity, thanks to the
We benefit from skilled and dedicated employees with great passion who played
a significant role in research and development of new products, strict quality
control, and improving customer satisfaction.
A Good Beginning Makes a Good Ending
P.I.P. pipettes are designed to deliver superior performance and accuracy year
after year. The high quality and long-term reliability of P.I.P. pipettes are highly
based upon the design, the quality of components and the entire controlled
production process. The specifications of this ergonomic and well-designed
pipette meet ISO 8655.
We Offer Complete Sales Support to Our Customers
products.
with any problems and provide after-sale and routine services for P.I.P. pipettes.
Introduction......................................................6
P.I.P. Pipette Description ..............................8
Pipetting Techniques ....................................12
Proper PipettingAdvices ...............................16
Cleaning and Maintenance .........................20
Decontamination............................................21
Autoclaving....................................................23
Pipette Service ...........................................24
Shipment .......................................................26
P.I.P. Pipette Serial Number ........................27
P.I.P. Pipette Warranty ................................28
Choosing the Proper Pipette ......................30
P.I.P. Pipette Technical Data ........................31
Contents
POLE IDEAL PARS
www.medpip.com
1. Introduction
Read this Operating Manual completely before using P.I.P. pipettes for the
first time.
ThisOperating Manualcontains allthe informationrequired for pipettemaintenance,
serviceintervals, decontamination, etc.So,keep it safeandhandy.
Enclose this Operating Manual when transferring the pipette to third parties.
Pole Ideal Pars Co. takes no responsibility for any damages arising from not
following the instructions of this Operating Manual.
1-1. How to Register
1. Visit my.medpip.com/en
2. Use the pipette Serial Number to register your device. )see page 27(
Register your pipette via www.medpip.com to
benefit your warranty period.
6
1-2. Symbols
1-3. Delivery Package
Table 1. P.I.P. Pipette Delivery Package
!
Product Qty.
Pipette
Operating Manual
Spare O-ring
Guarantee Card
Mini Brochure
1
1
1
5
1
1
7
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Ergonomic Finger Hook
2. P.I.P. Pipette Description
2-1. P.I.P. Pipette Overview
Push Button
Tip ejector
Tip ejector Button
Robust and Lightweight Body
Metal Tip Cone with O-rings for
optimal sealing and precision
Figure 1. P.I.P. Pipette Overview
8
2-2. Features
2-2-1. Pipetting System
Metal pipette parts, such as the tip cones, prevent breakage resulting from
accidental dropping and offer superior durability of the pipette.
The leak-tight O-rings prevent leakage and provide precise results.
2-2-2. Push Button
This ergonomic and accurate pipette has a unique design with detachable
push button. In the case a user switch to the left hand, the push button simply
can be taken out and turned in order to have a left-hand pipette with a noticeable
nominal volume on the push button.
The soft-touch push button of P.I.P. pipettes causes smooth pipetting and
reduces hand stress.
Color-coded cap is designed for easy volume identification.
2-2-3. Tip ejector Button
Separate tip ejector button with curved shape reduces
handmotion.
Figure 3. Buttons
Figure 2. Metal Pipetting System
9
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2-2-4. Body
High-Quality and robust body of P.I.P. pipette protects internal components
from damage if dropped.
Lightweight and ergonomic body of the P.I.P. pipette with balanced weight
P.I.P. pipettes are resistant to UV radiation and common laboratory chemicals,
organic solvents and detergents.
Ergonomic finger hook for comfortable handling
reduces hand stress.
P.I.P. Pipette is a fully autoclavable lab pipette
requires no disassembly before autoclaving. Just
simply take off the push button and put the pipette
in an autoclave.
0
C and a period of 20
minutes. )see page 23(
All P.I.P. pipettes have been engraved with a serial number on the body of the
pipette which can be used for registration, service and repair. )see page 27(
Figure 4. Robust and Lightweight Body
Pipette registration via my.medpip.com/en
guarantees you excellent after-sale services.
)see page 6(
10
2-3. Working Principles
P.I.P. pipette operates according to air cushion principles )air displacement
pipettes(. There is always a cushion of air )dead volume( between the piston
and the liquid. When the push button is pressed down, the piston inside the
instrument moves down to let air out. Air is displaced by the piston. The volume
of air displaced is equivalent to the volume of liquid aspirated. P.I.P. pipettes are
highly accurate for most pipetting applications.
Figure 5. Pipetting System
Piston
Air cushion
Shaft
Disposable tip
Sample
11
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3. Pipetting Techniques
Inordertopipettedifferent samples by P.I.P. pipettes, 2mainpipettingtechniques
1. Forward Pipetting Mode.
2. Reverse Pipetting Mode.
Use Forward Pipetting Mode for aqueous solutions.
Use Reverse Pipetting Mode for volatile and viscous samples.
Refer to table 2 for Proper Pipetting Techniques.
Solution / Compound Examples Pipetting Technique Comments
Aqueous Solution
Viscous Solution
Volatile Compounds
Body Fluids
Nucleotide Solutions
Radioactive Compounds
Acids/Alkalis
Buffers
Diluted Salt Solutions
Protein and Nucleic
Acid Solutions
Glycerol
Tween 20/40/60/80
Methanol
Whole Blood
Serum
Genomic DNA
PCR Products
14Carbonate
³H-thymidine
HSO
HCI
NaOH
__
Forward Pipetting
Reverse Pipetting
Reverse Pipetting
Reverse Pipetting
Forward Pipetting
Forward Pipetting
Forward Pipetting
Forward / Reverse
Pipetting
__
Pipette
slowly to avoid
bubble formation.
Pipette rapidly to
avoid evaporation.
Wait one second
after aspirationand
dispensing.
__
__
__
__
Table 2. Pipetting Techniques with P.I.P. Pipettes
12
3-1. Forward Pipetting
Press the push button smoothly to the First Stop.
Allow the push button to move up smoothly to the Rest
Position. Wait one second, so that the liquid has time to
move up into the tip.
While pipetting, hold the pipette vertically, so that the
tip does not touch the vessel wall. )figure 7(
Press down and/or release the push button smoothly
and consistently.
against the inside wall of the receiving vessel. Depress
the push button smoothly to the First Stop. )figure 8(
1 2 3 4 5
Rest Position
First Stop
Second Stop
Figure 7. Hold the pipette
vertically to aspirate
Figure 6. Forward Pipetting
Figure 8. DispensingAngle
13
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Before dispensing, carefully remove droplets
on the outside of the tip with a lint-free cloth,
beingsuretostay clear of the tip opening. )figure9(
button to the Second Stop. This purge stroke
removes any remaining sample from the tip.
Remove pipette tip end from sidewall by sliding
it up the wall.
the First Stop position. Repeat steps 2,3 and 4.
the Rest Position. Press the tip ejector button to
remove the tip. )figure 10(
Figure 9. Removing Droplets on
the Tip
Figure 10. Tip Ejection
14
3-2. Reverse Pipetting
smoothly to the Second Stop.
up smoothly to the Rest Position. Wait one second, so that all the liquid has time
to move up into the tip.
While pipetting, hold the pipette vertically, so that the tip does not touch the
vessel wall. )figure 7(
Press down and/or release the push button smoothly and consistently.
of the receiving vessel. Depress the push button smoothly to the First Stop. Wait
one second. )figure 8(
Before dispensing, carefully remove droplets on the outside of the tip with a
lint-free cloth, being sure to stay clear of the tip opening. )figure 9(
can be dispensed back into the original solution or thrown away.
steps 2 and 3.
ejector button to remove the tip. )figure 10(
1 2 3 4 5
Rest Position
First Stop
Second Stop
Figure 11. Reverse Pipetting
15
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4. Proper Pipetting Advices
To achieve the highest accuracy while using P.I.P. pipette, follow the proper
Choose the right pipette. The nominal volume written on your pipette should fit
the volume you plan to pipette.
Use single-use tips.
Figure 12. Evaluating tip quality
A smooth inner wall and
liquid in a tip.
A rough inner wall with
hang up liquid in a tip,
resulting in poor precision.
hang up liquid, resulting in
poor precision.
16
Figure 13. Press down with a
rotating motion. Figure 14. Avoid hammering on
the pipette tip.
Allow liquids and equipment to reach an equilibrium at an ambient temperature
beforeyou begin pipetting.
Pre-wet the pipette tip by aspirating and dispensing any sample at least 3 times
before aspirating it for delivery. This helps to prevent evaporation loss.
Immerse the tip to the proper depth during aspiration. )see table 3(
1. Hold the micropipette in one hand.
2. Lower the pipette into the tip.
To protect your pipette, avoid tapping the pipette onto the tip like a hammer.
17
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Choose the proper pipetting technique.
)see page 12(
gloves to reduce body heat transfer to the
pipette.
Put the pipette vertically on a pipette
stand when you are not pipetting. )figure 15(
!
penetration of liquids.
Do not set the pipette aside when the
pipette tip is filled. )figure 16(
Immersion DepthVolume
Table 3. Appropriate Immersion Depth
1 mm
2-3 mm
2-4 mm
3-6 mm
Figure 15. Proper Maintenance of P.I.P.
Pipettes.
18
4-1. Pipetting Influencing Parameters
To find out about the factors affecting pipetting accuracy refer to table 4.
Inuencing Parameters Eects Corrective Actions
Leaky/Poorly seated pipette tips
Reuse of pipette tips
The straightness of pipette tips
The difference in vapor pressure of
the liquid to be pipetted versus that of
the water used for calibration
Failure to wipe the pipette tip
on the vessel wall
Improper pipette tip immersion depth
Improper handling angle during pipetting
Irregular rhythm and timing during
pipetting
Uneven push button movement
A leaky pipetting system
0.5% to 50%
Up to 4%
Up to 10%
Up to 2%
Up to 3%
Up to 1%
Up to 1%
Up to 1.5%
Up to 0.5%
1% to 50%
Usingappropriateandrecommended
pipette tips )see page16(
Using pipette tips only once
Using standard tips only
)see page 16(
Sufficient pre-wetting of pipette tips
)see page 17(
Wiping of the pipette tip on the vessel
wall after dispensing the sample
Immerse the tip to the proper depth
during aspiration
)see page 18(
Holding pipette in a vertical position
while pipetting
Applying a consistent
pipetting technique
Smooth operation of push button
Send the pipette back to
P.I.P. company for service and repair
)see page 26(
Table 4. Pipetting Influencing Parameters
19
This manual suits for next models
39
Table of contents
