ibidi 11920 User manual

Instruction Manual
ibidi Gas Incubation System – Blue Line
Version 2.3
11920 ibidi Gas Incubation System for CO2– Blue Line
11922 ibidi Gas Incubation System for CO2and O2– Blue Line
.com
Flow
I 9.9
S[ 10.0] Run
CO2% Hum%
5.1
5.0 75.2
80.0
Setup

ibidi Gas Incubation System – Blue Line
Instruction Manual
Contact
ibidi GmbH
Lochhamer Schlag 11
82166 Gräfelfing
Germany
Phone: +49 89 / 520 46 17 - 0
Fax: +49 89 / 520 46 17 - 59
E-mail: inf[email protected]
Web: ibidi.com
Version 2.3 (October 18, 2023) © ibidi GmbH 3

ibidi Gas Incubation System – Blue Line
Instruction Manual
Contents
1 Preamble 6
1.1 Introduction ......................................... 6
1.2 Safety Symbols ....................................... 6
1.3 Nomenclature ........................................ 7
1.4 Specifications ........................................ 7
1.5 Disclaimer .......................................... 10
1.6 Safety Considerations .................................... 10
1.7 Limited Warranty ...................................... 12
1.8 Transporting the ibidi Gas Incubation System – Blue Line ................ 12
1.9 Repairing the ibidi Gas Incubation System – Blue Line ................. 12
1.10 Waste Disposal – WEEE/RoHS Compliance Statement ................. 13
1.10.1 EU Directive WEEE ................................. 13
1.10.2 EU Directive RoHS ................................. 13
1.11 Regulatory Statement .................................... 14
2 Intended Use 15
3 Principle 16
4 Equipment 18
4.1 Components of the ibidi Gas Incubation System – Blue Line .............. 18
4.2 Gas Mixer CO2or CO2/O2................................. 20
4.3 Humidifying Column ..................................... 21
4.4 Heated Tubing Package ................................... 22
4.5 Humidity Sensor ....................................... 23
5 Operation 25
5.1 Installation and Connection ................................. 25
5.1.1 Connection to the Gas Supply ........................... 26
5.1.2 Connection of the Heated Tubing Package and the Humidifying Column . . . 28
5.1.3 Connection of Humidity Sensor Cable ...................... 30
5.1.4 Connection of the Power Supply (and optionally USB) ............. 30
5.2 (Re)-Filling the Humidifying Column ............................ 31
5.3 Switching on the System .................................. 32
5.4 Setting Parameters With the Front Display ........................ 32
5.4.1 Run/Stop Mode ................................... 33
5.4.2 Incubation Parameters ............................... 33
5.4.3 Setup Menu ..................................... 34
6 IncuControl Software 36
4 © ibidi GmbH Version 2.3 (October 18, 2023)

ibidi Gas Incubation System – Blue Line
Instruction Manual
7 Maintenance 37
7.1 Disinfection and Cleaning .................................. 37
7.1.1 Wiping the Outer Surface ............................. 37
7.1.2 Cleaning the Column’s Inner Face ......................... 37
7.2 Influence of Surrounding Temperature and Airflow .................... 37
8 Troubleshooting 38
8.1 Controller Display Errors .................................. 38
8.2 Flow Readings ........................................ 38
8.3 CO2Channel Readings ................................... 39
8.4 O2Channel Readings .................................... 40
8.5 Humidity Channel Readings ................................ 41
8.6 Humidifying Column ..................................... 42
8.7 Focus not Stable ....................................... 43
8.8 Too High Evaporation .................................... 43
8.9 Condensation Inside the Stage Top Incubator ...................... 43
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ibidi Gas Incubation System – Blue Line
Instruction Manual
1 Preamble
1.1 Introduction
This manual is your guide for using the ibidi Gas Incubation System – Blue Line for cell culture
experiments on an optical microscope. It instructs first-time users on how to use the instrument, and
serves as a reference for experienced users.
Before using the ibidi Gas Incubation System – Blue Line, please read this instruction manual care-
fully and make sure that the contents are fully understood. This manual should be easily accessible
to the operator at all times during instrument operation. If this manual gets lost, see the online version
on ibidi.com or request a replacement via techsuppor[email protected].
To ensure safe operation, the ibidi Gas Incubation System – Blue Line must only be operated with
the supplied components and according to the instruction manual.
For Research Use Only! Not for use in diagnostic procedures.
1.2 Safety Symbols
Note that the signal words WARNING,CAUTION and NOTE have specific meanings in this man-
ual. Do not proceed beyond a signal word until you have performed the indicated actions. Warning
messages in the text are displayed in a gray shaded box. Please see Section 1.6 for general safety
considerations.
WARNING – A potentially hazardous situation which, if not avoided, could result in
serious injury or even death.
CAUTION – A potentially hazardous situation which, if not avoided, could result in
minor or moderate injury. It is also used to alert against damaging the equipment or
the instrument.
NOTE – Additional information to help achieve optimal instrument and assay perfor-
mance.
Symbols on the product identification label and back panel of the device:
CE Marking: This symbol indicates the product’s compliance with EU legislation.
This label is positioned on the back of the device and prompts you to read the manual
before using the device.
Product disposal: The symbol indicates that this product must be recycled/disposed
of separately from other household waste. See page 13 for details.
6 © ibidi GmbH Version 2.3 (October 18, 2023)

ibidi Gas Incubation System – Blue Line
Instruction Manual
1.3 Nomenclature
The ibidi Gas Incubation System – Blue Line consists of the following three main components:
Gas Mixer Humidifying Column Heated Tubing Package
1.4 Specifications
Table 1: Specifications of the ibidi Gas Incubation System – Blue Line
Electrical Specifications Power Supply
Protection class I
International protection marking (IEC
60529)
IP 20
Overvoltage category II
External power supply AC 100–240 V, 50/60 Hz, 1.4 A
Input line voltage Gas Mixer DC 24 V, 5 A, 120 W
Output voltage (to Humidifying Col-
umn)
DC 24 V, max. 3.6 A
Operating Conditions
Operating site Indoor use only
Operating temperature 18–30 °C/64–86 °F
Operating humidity max. 80 % relative humidity (RH)
Operating altitude max. 2000 m
(atmospheric pressure 800–1060 hPa/11.6–15.4 psi)
Storage Conditions -5–50 °C/23–122 °F
Humidity <60 % relative humidity (RH)
continued. . .
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ibidi Gas Incubation System – Blue Line
Instruction Manual
Table 1: (continued)
Outer Dimensions and Characteristics of the Components
Gas Mixer 90 × 170 × 230 mm3
2230 g/4.9 lbs
Humidifying Column 70 × 110 × 300 mm3
660/710/790 g /1.45/1.57/1.74 lbs (empty/min/max)
Heated Tubing Package 0.6 m (between Controller and Column)
1.5 m (between Column and Heated Lid)
82 g/0.18 lbs
Cable for Humidity Sensor 1.5 m
USB cable 1.8 m
Power supply cable 2.0 m (power supply to wall)
1.2 m (power supply to device)
Gas Connections
Gas tubing material Polyurethane, hydrolysis resistant
Gas tubing connections Push-in fittings
Gas Mixer input ID 4 mm
OD 6 mm
Gas Mixer output and ID 2.5 mm
Humidifying Column connection OD 4 mm
Connection to the Heated Lid Male Luer Lock
Gas Input Requirements
Compressed air Purity class: ISO 8573-1:2010 [1:2:1]
- from source with pressure reducer
(labline / gas bottle / Air Pressure
Generator 11929-230 or 11929-115)
1 bar/14.5 psi optimum (0.8–1.2 bar / 11.6–17.4 psi)
- from ibidi Air Pressure Generator
(11928-230)
300–400 mbar/4.35–5.8 psi
Carbon dioxide (CO2) Purity: >99 % CO2
from source with pressure reducer 1 bar/14.5 psi optimum (0.8–1.2 bar / 11.6–17.4 psi)
Nitrogen (N2)* Purity: >99 % N2
from source with pressure reducer 1 bar/14.5 psi optimum (0.8–1.2 bar / 11.6–17.4 psi)
*only for the CO2/O2version
CO2Control
Control range 0–15 %
Accuracy ±(0.2 % vol. + 3 % of reading)
e.g., ±0.35 % at 5 % CO2concentration
Resolution 0.1 %
continued. . .
8 © ibidi GmbH Version 2.3 (October 18, 2023)

ibidi Gas Incubation System – Blue Line
Instruction Manual
Table 1: (continued)
O2Control∗
Control range 1–21 % (note: settings below 1 % are less accurate and
a typical minimum value in an ibidi Heating Chamber is
0.5 % O2)
Accuracy ±0.2 % (typical)
±0.7 % (max.)
Resolution 0.1 %
Heat-up time Five minutes before proper operation of sensor
CO2cross sensitivity The O2sensor has some cross-sensitivity to CO2gas at
0 % O2concentration. This manifests as an offset in the
measured O2concentration, with typical values shown in
the table below:
CO2Conc. Set O2Conc. Displayed
O2Conc.
Actual
O2Conc.
0 % 0 % 0.1 % 0.1 %
5 % 0 % 0.5 % 0.1 %
10 % 0 % 0.6 % 0.1 %
Sensor lifetime Three years under continuous operation
*only for the CO2/O2version
Humidity Control
Humidity sensor type Sensirion SHT31
Control range 20–99 % (lower limit dependent on environment and gas
supply, min. set humidity is 0 %)
Sensor accuracy ±2.5 % (max.)
Resolution 0.1 %
Humidifying Column Temperature Control*
Control range 45 °C to 55 °C
Accuracy ±2 °C
Resolution 0.1 %
*only controllable on the Gas Mixer itself, not in IncuControl
Flow Control Mixed Gas
Control range 0–20 l/h
Accuracy ±25 %
Resolution 0.1 %
Humidifying Column
Liquid Ultra-pure water
Minimum volume 50 ml
Maximum volume 130 ml
Refilling 80 ml (approximately after 7 days at 10 l/h)
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ibidi Gas Incubation System – Blue Line
Instruction Manual
1.5 Disclaimer
• ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of
product use.
• The contents of this manual are subject to change without notice for product improvement.
• This manual is considered complete and accurate at publication.
• This manual does not guarantee the validity of any patent rights or other rights.
• If an ibidi software program doesn’t function properly, this may be caused by a conflict from
another program operating on the computer. In this case, take corrective action by uninstalling
the conflicting product(s).
• ibidi is a registered trademark of ibidi GmbH in Germany and other countries.
1.6 Safety Considerations
WARNING
• Only operate the ibidi Gas Incubation System – Blue Line with the supplied components.
• Only use the cables and plugs delivered with the system. The power plug of the control unit
must be inserted in an outlet with a ground (earth) contact.
• Do not replace detachable power cables by power cables with inadequate specifications. By
violating these instructions, you risk electric shock and fire.
• Only use extension cables that have a protective ground wire.
• Do not operate the ibidi Gas Incubation System – Blue Line under conditions that pose a risk
of explosion, implosion, or the release of gases.
• Do not place flammable solids, liquids, gases, or gas outlets near the system (e.g., matches,
ethanol, propane gas, solvents). Do not bring these products in contact with any other compo-
nent of the system either.
• Do not operate a damaged ibidi Gas Incubation System – Blue Line. If the housing seems
damaged or something is rattling inside the controller, contact the ibidi technical support for
repair.
• Some accessible parts of the ibidi Gas Incubation System – Blue Line (Humidifying Column
base, water inside the column, and Heated Tubing Package) can reach temperatures up to
55°C/131°F (60°C/140°F the water inside the column). Avoid touching the temperature-controlled
parts of the system when hot.
• The use of medical gases must take place in rooms with appropriate air exchange in order to
avoid harmful gas concentrations.
10 © ibidi GmbH Version 2.3 (October 18, 2023)