NUVE NF 1200 User manual

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NÜVE SANAYİ MALZEMELERİ İMALAT VE TİCARET A.Ş.
NF 1200 / 1200 R
MULTI-PURPOSE BENCH
TOP CENTRIFUGES
USER’S MANUAL
Z14. K25 249 Rev. No: 07 Rev.Date: 01/2016

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MANUFACTURER :
NÜVE SANAYİ MALZEMELERİ
İMALAT VE TİCARET A.Ş.
Saracalar Mah. Saracalar Kümeevleri No: 4/2
Akyurt 06750 Ankara-TURKEY
TEL: +(90) 312 399 28 30 (pbx)
FAX: +(90) 312 399 21 97
E-mail:[e-mail]
WARRANTY CERTIFICATE
1. Nüve warrants that the equipment delivered is free from defects during material and
workmanship. This warranty is provided for a period of two years. The warranty period
begins from the delivery date.
2. Warranty does not apply to parts normally consumed during operation or general
maintenance or any adjustments described in the operating instructions provided with the
equipment.
3. Nüve does not accept any liability in the case where the goods are not used in accordance
with their proper intent.
4. The warranty may not be claimed for damages occurred during the shipment, for damages
resulting from improper handling or use, the defects in maintenance, negligence, bad
functioning of auxiliary equipment, in the case of force majeure or accident and incorrect
power supply.
5. In the event of failure, Nüve shall be under no liability for any injury, or any loss or damage
as the result of the failure other than the guarantee conditions.
BEFORE OPERATING THE INSTRUMENT THIS MANUAL SHOULD BE READ
CAREFULLY.
THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF THE
INSTRUCTIONS AND PRECAUTIONS DESCRIBED IN THIS MANUAL.
INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF NÜVE. IT MAY
NOT BE DUPLICATED OR DISTRIBUTED WITHOUT PERMISSION.

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Dear Nüve User,
We would like to take this opportunity to thank you for preferring this Nüve product. Please read
the operating instructions carefully and keep them handy for future reference.
Please detain the packing material until you see that the unit is in good condition and it is operating
properly. If an external or internal damage is observed, contact the transportation company
immediately and report the damage. According to ICC regulations, this responsibility belongs to the
customer.
While you are operating the instrument please;
•obey all the warning labels,
•do not remove the warning labels,
•do not operate damaged instrument,
•do not operate the instrument with a damaged cable,
•do not move the instrument during operation.
In case of a problem contact your Nüve agent for an authorized service or maintenance.
The validity of the guarantee is subject to compliance with the instructions and precautions
described in this manual.
Nüve reserves the right to improve or change the design of its products without any obligation to
modify previously manufactured products.
Information contained in this document is the property of Nüve. It may not be duplicated or
distributed without its permission.
PLEASE REGISTER ONLINE TO VALIDATE WARRANTY:
To register your warranty online, please visit our web page www.nuve.com.tr and fill in WARRANTY
REGISTRATION FORM.

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TABLE OF CONTENTS
SECTION 1 ........................................................................................................................................................4
INTRODUCTION................................................................................................................................................4
1.1 USE AND FUNCTION .........................................................................................................................4
SECTION 2 ........................................................................................................................................................5
TECHNICAL SPECIFICATIONS.......................................................................................................................5
2.1 TECHNICAL SPECIFICATIONS TABLE.............................................................................................5
2.2 ACCESSORIES FOR NF 1200/1200R................................................................................................5
2.3 GENERAL PRESENTATION...............................................................................................................6
2.4 ROTOR SELECTION TABLE ..............................................................................................................7
2.5 PRECAUTIONS AND USAGE LIMITATIONS.....................................................................................8
SECTION 3 ........................................................................................................................................................9
SYMBOLS .........................................................................................................................................................9
SECTION 4 ........................................................................................................................................................9
OPERATING UNIT ............................................................................................................................................9
4.1. OPERATING........................................................................................................................................9
4.2. DISPLAYS AND CONTROL PANEL..................................................................................................10
4.2.1. Introduction to Control Display Panel Components and Functions............................................10
4.3. MAKING ANEW PROGRAM.............................................................................................................11
4.4. RUNNING THE PROGRAM ..............................................................................................................13
4.4.1. SELECTING THE MEMORIZED PROGRAM............................................................................13
4.5. SAFETY INTERLOCK SYSTEM........................................................................................................14
4.6. IMBALANCE DETECTION SYSTEM.................................................................................................14
4.7. MANUAL LID OPENING....................................................................................................................14
SECTION 5 ......................................................................................................................................................15
OPERATING PRINCIPLES.............................................................................................................................15
5.1. PREPARATION OF THE ROTORTO RUN.......................................................................................15
5.2. LOADING...........................................................................................................................................15
5.3. ROTOR INSTALLATION ...................................................................................................................16
5.4. DRIVE SYSTEM................................................................................................................................17
SECTION 6 ......................................................................................................................................................17
CLEANING AND PERIODIC MAINTENANCE ...............................................................................................17
6.1. PERIODIC MAINTENANCE..............................................................................................................17
6.2. STERILIZATION................................................................................................................................17
6.3. CORROSION INFORMATION ..........................................................................................................17
6.3.1. Chemical Corrosion....................................................................................................................18
6.4. STRESS CORROSION............................................................................................................................18
6.5. CLEANING ........................................................................................................................................18
6.6. ELECTRICITY ...................................................................................................................................18
SECTION 7 ......................................................................................................................................................19
DISPOSAL MANAGEMENT CONCEPT.........................................................................................................19
SECTION 8 ......................................................................................................................................................19
TROUBLESHOOTING.....................................................................................................................................19
SECTION 9 ......................................................................................................................................................20
ELECTRICAL CIRCUIT DIAGRAMS..............................................................................................................20
9.1. ELECTRICAL CIRCUIT DIAGRAM OF NF 1200...................................................................................20
9.2. ELECTRICAL CIRCUIT DIAGRAM OF NF 1200R ............................................................................21
SECTION 10 ....................................................................................................................................................22
WARNING LABEL...........................................................................................................................................22

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SECTION 1
INTRODUCTION
1.1 USE AND FUNCTION
The NF 1200 / NF 1200 R centrifuges are designed to separate liquids of different densities by
applying centrifugal force. Different types of rotors, which are swing-out and angle rotors, can be
fitted to the centrifuges.
The microprocessor control system of the centrifuges enables the user to observe and to control
the speed (RPM or RCF), the acceleration and braking rates, run time, program number and
temperature of the bowl for NF 1200 R. The microprocessor control system also displays the RPM
or RCF value during the run.
The ventilation system of the NF 1200 limits temperature in the bowl between the ambiant
temperature + 3 °C and ambiant temperature + 12 °C depending on the rotor type. The NF 1200 R
is equipped with a cooling system which provides to operate between -9 °C and + 40 ° C.
It ensures safe processes by means of the locking system which does not allow the centrifuge to
operate if the lid is open and which does not allow the rotor to spin if the lid is not closed.
The lid locking system which does not allow the centrifuge to operate if the lid is open, which also
does not allow the lid to be opened while the rotor is spinning and the possibility to open the lid by
pushing only one key when the program ends, provide safe and easy working conditions. There
are audible and visible alarms to inform the operator when the lid is open, when the program ends
and when any error conditions occur. In case of a power failure, the lid could be opened manually
by using a manual lid opening tool
The NF series centrifuges are manufactured according to the following standards,
EN 61010-1, EN 61010-2-020, EN 61000-6-3, EN 50419.
This device is in compliance with WEEE Regulation.

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SECTION 2
TECHNICAL SPECIFICATIONS
2.1 TECHNICAL SPECIFICATIONS TABLE
NF 1200
NF 1200 R
Max Speed
RA 280 rotor: 4100 rpm, MP 200 rotor: 4100 rpm,
RS 600 rotor: 9000 rpm, RS 100 rotor: 12000 rpm, RS24 rotor: 14000 rpm,
RS 30 rotor: 13500 rpm
Max. RCF
RA 280 rotor: 3082 x g, MP 200 rotor: 2349 x g, RS 600 rotor: 8965 x g, RS
100 rotor: 14167, RS24 rotor : 18405 x g, RS 30 rotor: 20170 x g
Max. Capacity
RA 280 rotor: 4x280 ml, MP 200 rotor: 2x3 microtitre plates,
RS 600 rotor: 6x100 ml, RS 100 rotor: 10x10 ml, RS 24 rotor: 24x1.5/2 ml,
RS 30 rotor: 30x2 ml
Control System
Microprocessor controlled
Display
High Visibility Digital
No. of Memories
10
Speed Range
500-14000 rpm
Speed Step
10 rpm
Speed Accuracy
± 20 rpm
Timer Range
01-99 min + Hold Position
Timer Step
1 min
Acceleration Rate
0: Slowest 9: Fastest
Braking Rate
0: Slowest 9: Fastest
Power supply
230 V, 50 Hz
Temp. range
-9° C / 40 ° C
Refrigerant Liquid
R134a
Temp. Accuracy
1°C
Temp. Performance
(at maximum speed) ambiant temp+12°C
+4°C at max. speed
Max. Power
500 W
800 W
Ext. Dimension
455 x 545 x 400
770 x 540 x 400
Packing Dim.
600 x 530 x 470
870 x 650 x 550
Net / Gross Weight
42 / 47 kg
75 / 80 kg
2.2 ACCESSORIES FOR NF 1200/1200R
A 08 062
Puller for rotor removal
B 50 016
RA 280 swing- out rotor 4*280 ml , 4.100rpm , 3.082 x g
G 51 007
Set of 4 buckes for RA 280
G 51 036
Set of 4 buckets with sealing cap
G 51 008
Set of 4 insert 1x280 ml
G 51 009
Set of 4 insert 1x250 ml flat
G 51 010
Set of 4 inserts 1x200 ml flat
G 51 011
Set of 4 inserts 1x100 ml
G 51 012
Set of 4 inserts 2x50 ml conical
G 51 013
Set of 4 inserts 4x25 ml
G 51 014
Set of 4 inserts 2x15 ml conical

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G 51 015
Set of 4 inserts 12x15 ml
G 51 016
Set of 4 inserts 12x5/7 ml
G 51 017
Set of 4 inserts 19x5 ml RIA
B 50 021
MP 200 microtitre rotor 4.100 rpm 2.349 xg
B 50 023
RS 600 Angle rotor 6x100 ml 9.000 rpm , 8.965 xg
G 51 018
Set of 6 adaptors 1x50 ml
G 51 019
Set of 6 adaptors 1x50 ml cocinal
G 51 020
Set of 6 adaptors 1x30/38 ml
G 51 021
Set of 6 adaptors 1x15 ml cocinal
B 50 022
RS 100 Angle rotor 10x10 ml 12.000 rpm ,14.167xg
G 51 022
Set of 6 adaptors 1x6 ml
G 51 023
Set of 6 adaptors 1x1.5/2 ml
B 50 014
RS 24 Angle rotor 24 x1,5/2 ml 14.000 rpm , 18.407xg
A 14 005
Adaptor 500/800 µl
A 14 006
Adaptor 200 µl PCR
A 14 007
Adaptor 250/400/700 µl
2.3 GENERAL PRESENTATION

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1
LID
7
On / off switch
2
Lock pin
8
Bowl
3
Gas spring
9
Electrıcal cables inlet and fuses
4
Gasket
10
Manual LİD opening hole
5
Rotor
11
Cooling unit
6
Dısplay and conrol panel
2.4 ROTOR SELECTION TABLE
SWING-OUT ROTORS AND ACCESSORIES
Rotor
Description
Capacity
Max.Tube
Dia.(mm)
Radius
(mm)
Max. Speed
(rpm)
Max.RCF
xg
RA280
Swing-out
Rotor
4x280ml
164
4,100
3,082
Set of 4
buckets
380 ml
nominal
76
Set of 4 inserts
1x280ml
65
Set of 4 inserts
1x250ml flat
62
Set of 4 inserts
1x200ml flat
55
Set of 4 inserts
1x100ml
39
Set of 4 inserts
2x50ml
conical
30
Set of 4 inserts
4x25ml
25
Set of 4 inserts
5x15ml
conical
17
Set of 4 inserts
12x15ml
17
Set of 4 inserts
12x5/7ml
13.5
Set of 4 inserts
19x5ml RIA
13
MP200
Microtitre Plate
Rotor
2 x 3
microtitre
plates
125
4,100
2,349

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ANGLE ROTOR ACCESSORIES
Rotor
Description
Capacity
Max.Tube
Dia(mm)
Max.Radius
(mm)
Max.Speed
(rpm)
Max
RCFxg
RS600
Angle Rotor
6x100ml
38
99
9,000
8,965
Set of 6 adaptors
1x50ml
30
Set of 6 adaptors
1x50 ml conical
30.5
Set of 6 adaptors
1x30/38ml
26
Set of 6 adaptors
1x15 ml conical
17
RS100
Angle Rotor
10x10ml
16
88
12,000
14,167
Set of 10
adaptors
1x6ml
13
Set of 10
adaptors
1x1.5/2ml
11
RS24
Angle Rotor
24x1.5/2ml
11
84
14,000
18,405
Adaptor
1x500/800µl
8
Adaptor
1x200 µl PCR
6.5
Adaptor
1x250/400/700
µl
6
RS30
Angle Rotor
30x2ml
11
99
13.500
20.170
Adaptor
1x500/800µl
8
Adaptor
1x200 µl PCR
6.5
Adaptor
1x250/400/700
µl
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2.5 PRECAUTIONS AND USAGE LIMITATIONS
Do not use the device for any purpose other than the usage purpose.
Prior to first use, the user’s manual should be read and the device is only to be used by
authorized and trained personnel. Only authorized technical personnel handle the product in
case of any failure.
The working bench should be durable to the device weight and vibration isolated.
Ensure that the rotor is placed correctly prior to usage.
According to the standard IEC 61010-2-020, anyone and any hazardous materials should not
be in the 300 mm safety zone while centrifuge is running.
Do not move the device while it is running.
Do not open the lid while rotor is spinning.
Apply the manual lid opening procedure in the case of power cut or in the case of any error.
Use only the the spare parts, rotors and accessories which are supplied by NUVE.
Load the rotor according to the explanations in the user’s manual.
Start the device after ensuring the rotor is loaded correctly.
Do not use the centrifuge in areas which are in explosive danger.
Do not centrifuge the explosive, flammable, radioactive, corrosive materials and the materials
which may react with each other.
The centrifuge and the rotor are not microbiologically leak-proof. Use tubes with leak-proof
covers, if hazardous, toxic and pathogenic microorganisms are centrifuged.
Do not use corrosive materials which may be harmful for the device integrity, rotor and
accessories.

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Do not use rotors and accessories with corrosion and mechanical damages.
Mains supply should be appropriate to power of the device and grounded.
Use tubes whose sizes are suitable to the rotor and accessories.
Tubes which are used in the centrifuge should not be deformed by the effect of the centrifuge
force.
Use glass tubes to balance, if glass tubes are used. Use plastics tubes to balance, if plastics
tubes are used.
Do not start the device unless tubes are in balance.
Imbalance loading may cause mixing the samples, broken tubes, and damages on the rotor
and motor shaft.
IF MENTIONED WARNINGS ARE NOT CONSIDERED, NÜVE WILL NOT BE RESPONSIBLE
FROM THEIR RESULTS.
SECTION 3
SYMBOLS
Symbol in the operating instructions:
Attention, general hazard area.
This symbol refers to safety relevant warnings and indicates possibly dangerous
situations.
The non-adherence to these warnings can lead to material damage and injury to
personal.

Symbol in the operating instructions:
This symbol refers to important circumstances.
SECTION 4
OPERATING UNIT
4.1. OPERATING
Open the lid and check that there is no sample in the instrument.
Turn the centrifuges on by using On/Off switch.
See that the display and control panel activates.
Learn the functions of the control panel (See Part 4.2.1).
Set the values and start the operation (See Part 4.3)