ZINEXTS ZiXpress 64 system User manual

Zinexts Life Science
Automated Nucleic Acid Purification System
ZiXpress 64 system
Catalog No.: ZP01014
Rev. Date: 24.05.2022
Manual No. QW-IH01-07
Version: 1.2
For General Laboratory Use Only.
Instruction Manual

Page 1
TABLE OF CONTENTS
Page #
1. Introduction..................................................................................................................................2
1.1 About ZiXpress 64 system............................................................................................................ 2
1.2 Technology Introduction.................................................................................................................. 2
1.3 Safety Information........................................................................................................................... 3
1.4 Important Instructions...................................................................................................................... 4
2. Intended Use & Specifications.......................................................................................6
2.1 Intended Use.................................................................................................................................... 6
2.2 Specifications................................................................................................................................... 6
2.3 Environment Requirements............................................................................................................ 6
2.4 Dimension and Weight.................................................................................................................... 7
3. Instrument Overview.............................................................................................................8
4. Installation.................................................................................................................................10
4.1 Composition of the ZiXpress 64 system.................................................................................... 10
4.2 Operating Environment / Conditions............................................................................................ 12
4.3 Initial Setting .................................................................................................................................. 14
4.4 Notes for Transportation / Shipping............................................................................................. 16
4.5 Functional Descriptions................................................................................................................. 17
5. Getting Started........................................................................................................................20
5.1 Preparation..................................................................................................................................... 20
5.2 Operation........................................................................................................................................ 21
5.3 Protocol Editing.............................................................................................................................. 28
6. Cleaning & Maintenance..................................................................................................30
7. Troubleshooting....................................................................................................................31
8. Contact Zinexts Life Science.......................................................................................32
9. Warranty Information.........................................................................................................33

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INTRODUCTION
About
ZiXpress 64 system
Thank you for purchasing ZiXpress 64 system for your
laboratory. This guide contains important information
regarding the safe use of the ZiXpress 64 system. Please
read this manual carefully, before starting to operate the
instrument for the first time, especially the Safety
Information.
If there is any question about how to install or operate it,
please contact our certified representatives / agents or email
our technical support center ([email protected]).
Technology Introduction
Principle
ZiXpress 64 system utilizes the unique characteristic of
magnetic beads bonding with nucleic acid under a specific
environment to purify the target nucleic acid samples. The
purification protocol includes four basic steps: sample lysis,
nucleic acid binding, wash and elution. Using ZiXpress 64
system, the majority of nucleic acid purification processes will
be done automatically inside of ZiXpress 64 system, it
dramatically reduce the human error and use the lab force
much more efficiently.
During the purification process, a 8-link rod comb, which is
attached to the tip comb rack, moves up and down repeatedly
to mix the reagents by creating turbulences in the 96 Deep-
Well Plate. When the permanent magnetic rods are insert into
the rod combs, the assembly lowers into the solution to
collect the magnetic beads at the bottom of the comb. The
whole assembly then move to a different Well and the
magnetic beads are then release by retracting the permanent
magnetic rods. ZiXpress 64 system equips an exclusive
temperature control module that perfectly connect with the 96
Deep-Well Plate to enhance the lysis and elution efficiency.
Applications
ZiXpress 64 system purifies DNA and RNA from a variety of
samples which are essential and broadly used in molecular
biology, genetic screening, sequencing, food safety, forensic,
etc.

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Safety Information
Attention
This chapter presents safety information that you need to be
familiar with and strictly follow before you operate, maintain or
service this instrument. If you failed to follow the safety
information, or neglected the warning in this user manual, it
might cause damage to the instrument. Please note that
operating the instrument outside its intended use might lead
to personnel injury and material loss.
For your safety and those of others, follow the guidelines
provided in the following pages concerning the use of the
ZiXpress 64 system.
ZINEXTS LIFE SCIENCE CORP. will not be held liable for
any errors, damage, or other unexpected events resulting
from miscarried following safety guideline.
Safety Symbols & Labels
Safety
The meanings of safety precaution marks are as follows:
Warning
“WARNING” indicates a dangerous condition that may lead to
death or serious injury.
Biohazard
This symbol is used to indicate that certain precautions must
be taken when working with potentially infectious material.
Caution
This symbol is used to indicate that non-compliance with
instructions or procedures may lead to physical injury or even
death or could cause damage to the instrument.
Important
“IMPORTANT” shows the important notes for usage, as well
as prohibited actions.
Note
“NOTE” indicates the notes, procedures that should be
obeyed and supplementary information for use.
Hot surface
This symbol is used to label potentially hot instrument
surfaces.

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Important Instructions
Read First!
For your safety and those of others, follow carefully the
guidelines provided in the following pages concerning the use
of the ZiXpress 64 system.
About Instrument
Warning:
◼Make sure the instrument is installed in a well ventilated
environment and away from any water source
◼Make sure the power source is matching the rating,
labelled on the instrument
◼Turn off, remove the power cord and cover the
instrument before a period of non-use or any
transportation
◼The power cord is one of the emergency power supply
control, please do not place the power cord in a location
hard to reach
◼If you heard or smelled anything during the operation,
please immediately disconnect the power and contact
your local representative / agent
Caution:
◼Never attempt to remodel the ZiXpress 64 system
without the manufacturer’s permission
◼Do not place or drop objects on the ZiXpress 64
system. Also, refrain from bumping or knocking it.
◼Repairs to the ZiXpress 64 system should be only
performed by representatives / agents that are
specifically authorized, and with original spare parts that
are certified by the ZINEXTS LIFE SCIENCE CORP.
◼If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment
may be impaired.
About Reagent Kits
Caution:
◼When handling any of the kits, refer to the respective
handbook.
◼Reagents in each kit should be handled by observing the

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safety information and precautions regarding the kit.
◼Extraction should be performed in an appropriate
laboratory or workspace.
◼Note:
◼Reagent kits will be generally not supplied with the
ZiXpress 64 system instrument. Please select your
desired Reagent Kits and order them separately.
About Samples
Biohazard:
Always wear appropriate gloves, a mask, and safety goggles
etc. when handling any infectious samples.
About Infectious
Wastes
When handling or disposing of infectious materials, follow the
laboratory guideline or the law regarding infectious waste to
perform proper incineration, fusion, sterilization, and/or
disinfection.
When you use a third party to dispose of it, outsource this
work an operator licensed to handle medical waste subject to
special control, and give them the manifest of the medical
waste at the same time.

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INTENDED USE & SPECIFICATIONS
Intended Use
This product and any of its accessories is for the extended
use of nucleic acid extraction, purification isolation in
molecular biology research laboratories and clinical
diagnosis laboratories.
Specifications
Product
Specifications
Model Name
ZiXpress 64 system
Instrument Type
Bench-top automated Nucleic
Acid Extractor
Sample Processing
1 - 64 samples per batch
Consumable
2.2 ml 96 Deep-Well Plate
Collection Efficiency
100 copies/mL, positive detecting
rates > 95%
Inter-Well Purification
Accuracy
CV < 3%
Heat Block Temperature
Room temperature to 80 °C
Mixing Speed
3 adjustable speeds
Reagent Type
Magnetic Beads
Control Interface
7-inch touch panel
Protocol
4 preset protocols;
16 user-defined protocols
Decontamination
Built-in UV light
Fuse
F3.15A 250V
Environment Requirements
Operating
Environment temperature:
10 - 40°C
Relative humidity:
< 80%
Power rating:
AC 100-240V, 400VA, UPS
optional
Storage
Environment temperature:
-20 - 55°C
Relative humidity:
≤80%

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Dimension & Weight
Dimensions:
722 x 360 x 490 mm
Weight:
38 kg
EMC
EN61326-1
Safety
EN60101-1, EN60101-2
Transportation
Environment temperature:
-20 - 55°C
Relative humidity:
< 80%

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INSTRUMENT OVERVIEW
Front view
Rear view
Touch Panel
Handler
Door
Window
Power inlet
Fixing block sensor
USB Port
Rear cover
Power switch

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Inside view
Magnetic Pillar
Mixing Sleeves
Reagent plate
Temperature Control
UV Lamp
Mixing Sleeves Track

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INSTALLATION
Composition of the ZiXpress 64 system
Packaging
Instrument
Check that the following parts are included in the package.
Contact your local representative if you notice any missing
part(s).
A.
B.
C.
D.
E.
F.
Quantity:
A. Instrument
x1
B. User Manual
x1
C. Power Cord
x1
D. 2.2ml Deepwell 96 Plate
x4
E. 8-Tip Comb
x4
F. Fixing block
x1
Please make sure all components are free of damages as soon
as you get the system. If any damage is found, please contact
your local representative for support.
Note:
Zinexts Life Science’s global warranty does not cover
transportation damages or improper handling and operation.

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Extraction Kits
Quantity:
A. 2.2ml Deepwell 96 Plate
x12 (plates)
B. 8-Tip Comb
x24 (pcs)
Note:
Extraction kits have to be purchased separately. Please contact
your local representative / agent for further information.
The contents of extraction kits are various. Refer to the Reagent
Kit Handbook, enclosed in the reagent box, for further details.

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Operating Environment / Conditions
ZiXpress 64 system
Operating
Environment
Use the ZiXpress 64 system system in an environment that
meets the following conditions:
◼The space for the allocation of ZiXpress 64 system must
secure 5-10 cm distance from the instrument to the wall.
◼A location where power can be provided.
◼A location with a temperature between 10 - 40°C, and a
humidity of < 80%RH (non-condensing)
◼A location with a stable temperature (Warming a cold room
suddenly or moving the instrument from a room with low
temperature to a warm room may cause condensation
inside the device, that might resulting in abnormal
extraction!)
◼A location that is flat and stable, without external vibrations
◼A location away from direct sunlight (If necessary, block
the sunlight by closing curtains or blinds)
◼A location which is well ventilated and not dusty
◼A location far from objects which generate strong magnetic
fields, as motors, transformers, TV, audio speakers,
magnets, etc. (Bringing the ZiXpress 64 system close to
any type of magnetic field may cause a malfunction.)
Warning:
Do not use the ZiXpress 64 system in an environment where

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it is wet or can be splashed with water. It may cause a device
failure, fire, or electric shock.
When relocating the ZiXpress 64 system, disconnect the
plug from the outlet first. If the power cable is damaged, this
may cause a device failure, fire, injury, or electric shock.
Caution:
Do not use the ZiXpress 64 system in an unstable place
such as a slanted surface or a place subject to vibrations. It
may cause injury or device failure.
Do not use the ZiXpress 64 system in direct sunlight or close
to a heating device. It may shorten the life of the ZiXpress 64
system, or cause trouble.
ZiXpress 64 system
Operating
Conditions
Items
Conditions
Temperature (°C)
During operation
10 - 40
Humidity (RH)
During operation
< 80%
Max. wet bulb
temperature (°C)
During operation
29 (non-
condensing)
During down time
29 (non-
condensing)
Temperature gradient (°C/hr)
12 or less
(non-condensing)
Humidity gradient (RH/day)
30 or less
(non-condensing)
Altitude, operating (m)
1600 or less

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Initial Setting
ZiXpress 64
system
Settings
(1) Open the packing box and take out the instrument and related
accessories.
Important:
The ZiXpress 64 system has a weight of more than 25kgs.
It should be lift and moved by two persons. Hold the moving
handler of the instrument from two sides to move it out of the
transportation box.
Do not hold the door or the plastic outer covering while
transporting the instrument.
Caution:
Improper handling of the movement of the ZiXpress 64
system will lead to instrument damages.
For correct and safe use of the ZiXpress 64 system, install it
in a location that is close to electrical outlet and has enough
space for installation and operation from the main switch.
Important:
Keep the shipping box and fixing block, they are needed for
transporting the ZiXpress 64 system.

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(2) Connect power cord to the instrument.
(3) Remove the Fixing Block inside the instrument and place it in
the Fixing Block Sensor Spot, following the guideline below:
1. Plug in the power cord to the electric outlet
2. Use a Phillips head screwdriver to release the red
screws
3. Remove the Fixing Block
4. Place the Fixing Block in the upper part of the back
of the instrument, in the Fixing Block Sensor Spot.
Without performing this step, the sensor will not
allow the instrument to operate.
2.
3.
4.
Power inlet

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Notes for Transportation / Shipping
ZiXpress 64 system
Transportation
When transporting / shipping the ZiXpress 64 system to a
new location, perform the following tasks:
1. Insert Fixing Block
(1) Put the Fixing Block to fix the Magnetic Pillar frame.
(2) Install the Fixing Block
(3) Use Phillips head screwdriver for the red screw
(4) Close the door
2. Disconnect the plug from the outlet and remove all
attached parts (Power Cord) from the instrument.
Warning:
If the power cable is damaged, this may cause a device
failure, fire, injury, or electric shock.

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3. Pack the ZiXpress 64 system instrument, including
accessories in its original shipping box or some other
equivalent parcel.
Functional Descriptions
Main Menu
Home
UV
Light
Light
Protocol
Edit
Setting
Hibernate

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Protocol Edit
After pressing , the icon will change into , and you
can select “1” to “12” to edit your protocol.
LED Light
After pressing button, the icon will change into ,
the instrument’s light will turn off.

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UV Light
After pressing button, Press <30 minutes> to start a
default 30 minutes decontamination process or press
<User setting> to set a desired time.
Settings
After pressing button, you can do the instrument
adjustment / check / test.
Sleeping mode
After pressing button, the instrument will get into the
“sleeping mode’’. If you want to wake up the instrument, touch
the screen again.
Note:
Before getting into the sleeping mode, please press
button first to turn off the LED light.