ZINEXTS ZiXpress 64 system User manual

Zinexts Life Science
Automated Nucleic Acid Purification System
ZiXpress 64 system
Catalog No.: ZP01014
Rev. Date: 24.05.2022
Manual No. QW-IH01-07
Version: 1.2
For General Laboratory Use Only.
Instruction Manual

Page 1
TABLE OF CONTENTS
Page #
1. Introduction..................................................................................................................................2
1.1 About ZiXpress 64 system............................................................................................................ 2
1.2 Technology Introduction.................................................................................................................. 2
1.3 Safety Information........................................................................................................................... 3
1.4 Important Instructions...................................................................................................................... 4
2. Intended Use & Specifications.......................................................................................6
2.1 Intended Use.................................................................................................................................... 6
2.2 Specifications................................................................................................................................... 6
2.3 Environment Requirements............................................................................................................ 6
2.4 Dimension and Weight.................................................................................................................... 7
3. Instrument Overview.............................................................................................................8
4. Installation.................................................................................................................................10
4.1 Composition of the ZiXpress 64 system.................................................................................... 10
4.2 Operating Environment / Conditions............................................................................................ 12
4.3 Initial Setting .................................................................................................................................. 14
4.4 Notes for Transportation / Shipping............................................................................................. 16
4.5 Functional Descriptions................................................................................................................. 17
5. Getting Started........................................................................................................................20
5.1 Preparation..................................................................................................................................... 20
5.2 Operation........................................................................................................................................ 21
5.3 Protocol Editing.............................................................................................................................. 28
6. Cleaning & Maintenance..................................................................................................30
7. Troubleshooting....................................................................................................................31
8. Contact Zinexts Life Science.......................................................................................32
9. Warranty Information.........................................................................................................33

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INTRODUCTION
About
ZiXpress 64 system
Thank you for purchasing ZiXpress 64 system for your
laboratory. This guide contains important information
regarding the safe use of the ZiXpress 64 system. Please
read this manual carefully, before starting to operate the
instrument for the first time, especially the Safety
Information.
If there is any question about how to install or operate it,
please contact our certified representatives / agents or email
our technical support center ([email protected]).
Technology Introduction
Principle
ZiXpress 64 system utilizes the unique characteristic of
magnetic beads bonding with nucleic acid under a specific
environment to purify the target nucleic acid samples. The
purification protocol includes four basic steps: sample lysis,
nucleic acid binding, wash and elution. Using ZiXpress 64
system, the majority of nucleic acid purification processes will
be done automatically inside of ZiXpress 64 system, it
dramatically reduce the human error and use the lab force
much more efficiently.
During the purification process, a 8-link rod comb, which is
attached to the tip comb rack, moves up and down repeatedly
to mix the reagents by creating turbulences in the 96 Deep-
Well Plate. When the permanent magnetic rods are insert into
the rod combs, the assembly lowers into the solution to
collect the magnetic beads at the bottom of the comb. The
whole assembly then move to a different Well and the
magnetic beads are then release by retracting the permanent
magnetic rods. ZiXpress 64 system equips an exclusive
temperature control module that perfectly connect with the 96
Deep-Well Plate to enhance the lysis and elution efficiency.
Applications
ZiXpress 64 system purifies DNA and RNA from a variety of
samples which are essential and broadly used in molecular
biology, genetic screening, sequencing, food safety, forensic,
etc.

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Safety Information
Attention
This chapter presents safety information that you need to be
familiar with and strictly follow before you operate, maintain or
service this instrument. If you failed to follow the safety
information, or neglected the warning in this user manual, it
might cause damage to the instrument. Please note that
operating the instrument outside its intended use might lead
to personnel injury and material loss.
For your safety and those of others, follow the guidelines
provided in the following pages concerning the use of the
ZiXpress 64 system.
ZINEXTS LIFE SCIENCE CORP. will not be held liable for
any errors, damage, or other unexpected events resulting
from miscarried following safety guideline.
Safety Symbols & Labels
Safety
The meanings of safety precaution marks are as follows:
Warning
“WARNING” indicates a dangerous condition that may lead to
death or serious injury.
Biohazard
This symbol is used to indicate that certain precautions must
be taken when working with potentially infectious material.
Caution
This symbol is used to indicate that non-compliance with
instructions or procedures may lead to physical injury or even
death or could cause damage to the instrument.
Important
“IMPORTANT” shows the important notes for usage, as well
as prohibited actions.
Note
“NOTE” indicates the notes, procedures that should be
obeyed and supplementary information for use.
Hot surface
This symbol is used to label potentially hot instrument
surfaces.

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Important Instructions
Read First!
For your safety and those of others, follow carefully the
guidelines provided in the following pages concerning the use
of the ZiXpress 64 system.
About Instrument
Warning:
◼Make sure the instrument is installed in a well ventilated
environment and away from any water source
◼Make sure the power source is matching the rating,
labelled on the instrument
◼Turn off, remove the power cord and cover the
instrument before a period of non-use or any
transportation
◼The power cord is one of the emergency power supply
control, please do not place the power cord in a location
hard to reach
◼If you heard or smelled anything during the operation,
please immediately disconnect the power and contact
your local representative / agent
Caution:
◼Never attempt to remodel the ZiXpress 64 system
without the manufacturer’s permission
◼Do not place or drop objects on the ZiXpress 64
system. Also, refrain from bumping or knocking it.
◼Repairs to the ZiXpress 64 system should be only
performed by representatives / agents that are
specifically authorized, and with original spare parts that
are certified by the ZINEXTS LIFE SCIENCE CORP.
◼If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment
may be impaired.
About Reagent Kits
Caution:
◼When handling any of the kits, refer to the respective
handbook.
◼Reagents in each kit should be handled by observing the

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safety information and precautions regarding the kit.
◼Extraction should be performed in an appropriate
laboratory or workspace.
◼Note:
◼Reagent kits will be generally not supplied with the
ZiXpress 64 system instrument. Please select your
desired Reagent Kits and order them separately.
About Samples
Biohazard:
Always wear appropriate gloves, a mask, and safety goggles
etc. when handling any infectious samples.
About Infectious
Wastes
When handling or disposing of infectious materials, follow the
laboratory guideline or the law regarding infectious waste to
perform proper incineration, fusion, sterilization, and/or
disinfection.
When you use a third party to dispose of it, outsource this
work an operator licensed to handle medical waste subject to
special control, and give them the manifest of the medical
waste at the same time.

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INTENDED USE & SPECIFICATIONS
Intended Use
This product and any of its accessories is for the extended
use of nucleic acid extraction, purification isolation in
molecular biology research laboratories and clinical
diagnosis laboratories.
Specifications
Product
Specifications
Model Name
ZiXpress 64 system
Instrument Type
Bench-top automated Nucleic
Acid Extractor
Sample Processing
1 - 64 samples per batch
Consumable
2.2 ml 96 Deep-Well Plate
Collection Efficiency
100 copies/mL, positive detecting
rates > 95%
Inter-Well Purification
Accuracy
CV < 3%
Heat Block Temperature
Room temperature to 80 °C
Mixing Speed
3 adjustable speeds
Reagent Type
Magnetic Beads
Control Interface
7-inch touch panel
Protocol
4 preset protocols;
16 user-defined protocols
Decontamination
Built-in UV light
Fuse
F3.15A 250V
Environment Requirements
Operating
Environment temperature:
10 - 40°C
Relative humidity:
< 80%
Power rating:
AC 100-240V, 400VA, UPS
optional
Storage
Environment temperature:
-20 - 55°C
Relative humidity:
≤80%

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Dimension & Weight
Dimensions:
722 x 360 x 490 mm
Weight:
38 kg
EMC
EN61326-1
Safety
EN60101-1, EN60101-2
Transportation
Environment temperature:
-20 - 55°C
Relative humidity:
< 80%

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INSTRUMENT OVERVIEW
Front view
Rear view
Touch Panel
Handler
Door
Window
Power inlet
Fixing block sensor
USB Port
Rear cover
Power switch

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Inside view
Magnetic Pillar
Mixing Sleeves
Reagent plate
Temperature Control
UV Lamp
Mixing Sleeves Track