Quansys Q-View IMAGER LS User manual

Version 2.03
For Research Use Only
Not For Use in Diagnostic Procedures
Operator’s Manual

04 For Research Use Only
Introduction
The Q-View™ Imager LS is designed to acquire
high quality images of chemiluminescent 96-well
microtiter plates. It is intended for research use only.
Q-View Software is intended to be used to control the
instrument and for the analysis of data produced by
the Q-View Imager LS.
Notes on Safety
The Q-View Imager LS has been designed and
certied to meet EN61010-1 requirements, which are
internationally accepted electrical safety standards.
Certied products are safe to use when operated in
accordance with the device instruction manual. It is
critical that the user read this manual in its entirety
and train on the device frequently to ensure proper
operation. For laboratory use only, Pollution Degree
2 or better. Recommended for use below 2000 m. If
the device is used outside of the instructions herein
or modied, dissembled, or altered, the safety of the
device cannot be guaranteed. Improper operation
may:
1. Void the manufacturer’s warranty
2. Void the regulatory certication
3. Create a potential safety hazard
Manual Markings
Indicates a warning: Warnings help prevent safety
issues or failures in performance.
Indicates an important notication: Important
notications alert the user to certain steps that are
critical for optimal performance.

05For Research Use Only
Notice of Warranty
THIS LIMITED WARRANTY COVERS NORMAL USE.
QUANSYS BIOSCIENCES DOES NOT WARRANT
AND IS NOT RESPONSIBLE FOR DAMAGES CAUSED
BY MISUSE, ABUSE, ACCIDENTS, VIRUSES,
UNAUTHORIZED SERVICE OR PARTS, OR THE
COMBINATION OF QUANSYS BIOSCIENCES BRANDED
PRODUCTS WITH OTHER PRODUCTS. THIS LIMITED
WARRANTY DOES NOT COVER NON-QUANSYS
BIOSCIENCES BRANDED PRODUCTS. ANY WARRANTY
APPLICABLE TO NON-QUANSYS BIOSCIENCES
BRANDED SOFTWARE OR PRODUCTS IS PROVIDED
BY THE ORIGINAL MANUFACTURER.
Printing History
Version Number: Date:
1.0 September 2015
1.1 July 2020
1.2 April 2021
2.0 July 2022
Contact Information
Mailing Address:
Quansys Biosciences
365 North 600 West
Logan, Utah 84321
Phone Numbers:
U.S. Toll Free: [phone]
Direct: [phone]
Fax: [phone]
Email Addresses:
General Information: [e-mail]
Sales: [email protected]
Technical Support: [email protected]

06 For Research Use Only
TABLE OF CONTENTS
Introduction IV
Notes on Safety IV
Manual Markings IV
Notice of Warranty IV
Printing History V
Contact Information V
Chapter 1: Installation and Safety
Information 2
Safety Precautions 2
Lid and Glass/Tray Insert 2
Electricity 2
Placement in the Laboratory 2
Temperature and Humidity 2
Ventilation and Dust 2
Space 3
Vibration 3
Q-View Imager LS Setup 3
Assembly 3
Focusing the Q-View Imager LS 4
Light Leak Check 4
Acquire Dark Field 4
Setup Completion 5
Disassembly 5
Moving the Q-View Imager LS 5

1For Research Use Only
Chemical Safety 6
Electronic Considerations 6
Power Cord 6
USB Cord 7
Routine Maintenance 7
Cleaning 7
Chapter 2: System Overview 8
External Features 8
Back 8
Computer Connection 8
Q-View Software Introduction 8
Chapter 3: Operation 9
Before you Begin 9
Cleaning the Tray Surface 9
Loading the Plate 9
Imaging the Plate 9
Using the Qualication Plate 10
Troubleshooting 10
Troubleshooting Guide: 11
Appendix 12
Q-View Imager LS Specications 12
Operating Specications 12
Plate Diagram 18
Notes 19

2For Research Use Only
Chapter 1: Installation
and Safety Information
SAFETY PRECAUTIONS
Lid and Glass/Tray Insert
Make sure the lid is open and is not moving when
placing the plate in the tray. Also, if removing the
glass/tray insert for cleaning, exercise caution that
nothing is dropped on the lens of the camera.
Electricity
Since the Q-View Imager LS has a 110V/220V power
supply, exercise typical precautions to ensure shocks
do not occur. Do not place liquids close to the Q-View
Imager LS and do not cut cords that provide power or
connect the device to the PC.
PLACEMENT IN THE
LABORATORY
Temperature and Humidity
The Q-View Imager LS has a high resolution camera,
which may be subject to performance failures if the
temperature is too high. If the lab temperature is too
high, the camera may be unable to perform correctly.
Recommended operating conditions are 15–35°
C. Humidity can also cause operation problems
for the Q-View Imager LS. Ensure your laboratory
environment does not exceed a dew point of 20° C to
prevent condensation.
Ventilation and Dust
Ensure the room where the Q-View Imager LS is
placed has adequate ventilation to ensure passive
cooling can occur. Dust can collect on the glass on

3For Research Use Only
the tray deck and cause dust particles to be visible in
your image. Please take precautions to keep the lab
bench surface and oors free from dust.
Space
The footprint of the Q-View
Imager LS is 25.4 W x 23.5
D x 55.88 H cm (10 x 9.25 x
22 inches). Ensure the space
around the Q-View Imager
LS is free of chemicals that
can spill or other equipment
that can fall and damage the
Q-View Imager LS.
Vibration
The exposure times of the Q-View Imager LS can be
up to 5 minutes. It’s important that the location of the
Q-View Imager LS is free of excessive vibration. Make
sure equipment that might cause vibrations such as
vortex mixers, shakers, centrifuges, etc. are not co-
located on the bench with the Q-View Imager LS. Also
consider where the Q-View Imager LS is placed in
the laboratory. For example, the Q-View Imager LS
should not be placed on a bench near a door that
could be slammed or on a bench near a desk where
someone could bump it during exposures.
Q-VIEW IMAGER LS SETUP
Assembly
The Q-View Imager LS weighs approximately 9.12 kg
(20.12 lbs). To correctly setup the Q-View Imager LS
the following steps should be followed:
1. Place the Q-View Imager LS in a clean work area.
Ensure the location meets the requirements
addressed in Placement in the Laboratory ()
2. Connect the wall cord to the Q-View Imager LS..
3. Plug the wall cord into a surge protector.
4. Connect the USB cord to the computer that will

4For Research Use Only
be running Q-View™ Software.
5. Open Q-View Imager LS lid, ensure the enclosure
where the glass/tray insert ts is free from
debris and liquids.
6. Clean the Q-View Imager
LS glass within the glass/
tray insert with a lint-
free cloth and ethanol,
rubbing alcohol, or
window cleaner.
7. Place the glass/tray insert
on the Q-View Imager
LS with the glass on the
bottom.
8. Open Q-View Software.
The connected imager should automatically be
detected and a dialog box should open with a
link to Manage Imagers. If this does not occur,
in the Settings menu, click Manage Imagers.
9. From the Manage Imagers menu the connected
imager may be selected, named, focused and a
dark eld acquired.
Focusing the Q-View Imager LS
Open the Q-View Imager LS lid. Place the 96-well
focusing plate (included) on the plate tray. From the
Manage Imagers menu, select Focus. With the lid
still open, use the + and – buttons on the software
to bring the words printed on the focusing plate into
focus if needed. You can click on the image to zoom in
or out. When zoomed in, right-click and drag to scroll.
Light Leak Check
Upon completing the Q-View Imager LS installation
it may be necessary to ensure there are no light
leaks. To perform this test remove any plates from
the Q-View Imager LS, ensure the lid is closed, and
capture an image. The average pixel intensity of the
image should be less than 500. If the pixel intensity
is above this specication, please contact customer
support at Quansys Biosciences 1-888-QUANSYS
(782-6797) or [email protected].

5For Research Use Only
Acquire Dark Field
Dark Field Image Acquisition minimizes background
noise in subsequent plate images. Remove any plates
from the Q-View Imager LS, close the Q-View Imager
LS lid, and click OK on the software. In the Settings
menu click on Manage Imagers. Select the correct
imager and press Acquire Dark Field. The process
will take approximately ve minutes.
Setup Completion
Once the Q-View Imager LS setup is nished, the
software opens with the Update Preferences dialog
box. Select your preferences and log in if user accounts
were enabled. If your Internet connection on any
computer running Q-View Software goes through a
proxy, specify these settings under Administration
> Network Settings. Your Q-View Software and
Q-View Imager LS are now ready to use.
Disassembly
Carefully remove the glass/
tray insert from the Q-View
Imager LS. Take caution that,
while removing the glass/tray
insert, nothing is dropped
into the Q-View Imager LS
cavity. Anything dropped will
fall directly onto the lens of
the camera and cause damage
which will adversely aect the
Q-View Imager LS.
MOVING THE Q-VIEW IMAGER
LS
Ensure there is no plate on the tray of the Q-View
Imager LS and unplug all cords. The Q-View Imager
LS weighs approximately 9.12 kg (20.12 lbs).
In order to move or ship the Q-View Imager LS, there
are several critical steps that should take place.

6For Research Use Only
1. Ensure the new location meets the requirements
addressed in Placement in the Laboratory
(page 2)
2. Remove any remaining
plates on the tray
3. Remove the glass/tray
insert
4. Close Q-View Software on
the PC
5. Remove all cords from
the device
6. With a piece of tape, tape
the lid down so it doesn’t
open during transit
7. Insert the Q-View Imager LS into the provided
plastic sleeve
8. Insert the glass/tray insert into the protective
sleeve
9. Bundle and tie cables together
10. Insert the Q-View Imager LS into the foam
insert in the Q-View Imager LS box
11. Place the glass/tray insert, and cords into the
box
CHEMICAL SAFETY
The only chemicals used with the Q-View Imager LS
are deposited within the wells of the plate, which is
placed on the glass/tray insert. It is important to take
precaution that these chemicals do not spill onto the
glass/tray insert. If any spillage occurs on the glass/
tray insert, clean it immediately before closing the
lid. If liquids are present on the glass/tray insert,
they may drip onto the camera lens and damage the
Q-View Imager LS.

7For Research Use Only
ELECTRONIC CONSIDERATIONS
Power Cord
The power cord is a 3-wire grounding-type plug.
This cord will only t into a grounded outlet, which
is a requirement for the Q-View Imager LS. Do not
remove the grounding leg of the plug. Doing so will
void all warranties on the Q-View Imager LS.
The power cord provided with the Q-View Imager LS
is specic to the power supplied in your region. If you
have a dierent voltage supply or need a dierent
power cord for any reason, please contact technical
support. Do not use an extension cord for the Q-View
Imager LS. Ensure that all equipment plugged into
the same circuit as the Q-View Imager LS does not
exceed the amperes of service, or you may experience
a tripped breaker or power failure that could result in
the loss of your image.
Before unplugging the power cord, make sure Q-View
Software is closed on the PC. Make sure the USB cord
is unplugged. At this point, it is safe to remove the
power cord.
USB Cord
The USB cord connects the Q-View Imager LS to the
PC, which controls and operates the Q-View Imager
LS. This cord should only be unplugged from the
Q-View Imager LS once Q-View Software is closed.

8For Research Use Only
ROUTINE MAINTENANCE
Cleaning
Cleaning is recommended before every use. Ensure
that the glass/tray insert is free from dust and any
chemicals. To clean the device, use 70 percent
ethanol or warm water. Do not use scouring cleaners
or abrasive pads as this can scratch the glass. Be
careful not to spill any cleaning materials inside the
Q-View Imager LS.
Regularly inspect all cables for fraying or loose
connections.

9For Research Use Only
Chapter 2: System
Overview
EXTERNAL
FEATURES
Back
There are three features on
the back of the Q-View Imager
LS. A power plug receptacle,
the USB cord receptacle,
and a label containing the
serial number and power
specications for the unit.
COMPUTER CONNECTION
The Q-View Imager LS is connected to the PC via a
USB cable. Do not unplug either end of the USB cable
without closing Q-View Software on the PC. From
within Q-View Software, the user can open Imager
Management and see an entry in a table for the
Q-View Imager LS with one of the columns saying
“connected” or “disconnected.”
Q-VIEW SOFTWARE
INTRODUCTION
A copy of Q-View Software will accompany the
Q-View Imager LS. To download and install Q-View
Software from online, you must request a license key
from customer support ([e-mail]). You
may also request a copy of Q-View Software from:
quansysbio.com/request-software.
Please contact our technical support sta at support@
quansysbio.com for more assistance with Q-View
Software.
Note: The label
is placed on the
back panel and
contains the serial
number along with
power specications
including voltage,
current, and
frequency.

10 For Research Use Only
Chapter 3: Operation
BEFORE YOU BEGIN
The following procedure is recommended before
every use.
1. Clean the top of the glass/tray insert and ensure
it is free of debris.
2. Check within Q-View Software that the Q-View
Imager LS is connected to the PC.
CLEANING THE TRAY SURFACE
If any particulate, liquid, or dried salt residues or
smudges are left on the glass or the tray, it will
adversely aect the quality of the image and the
overall quality of your data. This can be cleaned with
70 percent ethanol using a lint-free cloth. Do not use
scouring cleaners or abrasive pads as this can scratch
the glass.
LOADING THE PLATE
The user sets the Q-Plex™ plate in the glass/tray
insert with well A1 in the top left corner of the tray
when facing the Q-View Imager LS. Make sure the
plate rests at on the glass on all edges of the plate.
Make sure there is no lid on the plate and there is
only one plate on the tray. Then, gently close the lid
and proceed to image.

11For Research Use Only
IMAGING THE PLATE
Within Q-View Software, the user will select Image
Processing and select the tab Acquire Image. The
name of the connected imager should appear in the
dropdown box under Imager. If the dropdown says
“No Imagers Connected”, select Refresh. The name
of the Q-View Imager LS will appear and connection
can be made.
At this point, the user may select the Exposure Time
and Image Name. Once these settings are made,
click on Capture Image(s) and Q-View Software will
image the plate that has been inserted.
USING THE QUALIFICATION
PLATE
The optional Q-View™ Qualication Plate can be used
to qualify the performance of the Q-View Imager LS
over time. This plate has built-in LEDs that create
a constant signal that can be imaged over certain
periods of time with Q-View Imagers. Parameters
can be imported or determined dynamically by
Q-View Software to determine acceptable levels of
performance.
As an example of how to use the Q-View Qualication
Plate, for Quansys in-house purposes, 10 images
are taken of the Qualication Plate. Acceptable
parameters are established by taking the average of
the 10 images, plus or minus two times the standard
deviation of each window read by the Q-View Imager
LS.
To purchase or learn more about the Q-View
Qualication Plate, please contact Quansys sales or
technical support.
Note: The
qualication process
will take 45 minutes
to complete.

12 For Research Use Only
TROUBLESHOOTING
To resolve any issue with the Q-View Imager LS,
please contact Quansys Biosciences by calling
1-877-QUANSYS or send an email describing the
issue to [email protected]. If outside the
USA, please contact your Quansys distributor for
your area. Have your serial number available with a
detailed description of the issue. The serial number is
located on the label on the back of the device.
Troubleshooting Guide:
Issue Problem Solution
Q-View
Software is
unable to
recognize
the Q-View
Imager LS
Failure of
communication
between
the Q-View
Imager LS and
computer
• Ensure power is
supplied to the
Q-View Imager
LS
• Ensure the
USB cable is
connected to
both the Q-View
Imager LS and
the computer
• Disconnect
the Q-View
Imager LS USB
cable from the
computer and
restart the
computer
Plate images
are blurry
The Q-View
Imager LS is
out of focus
Using the focus
functionality
built into Q-View
Software, focus the
Q-View Imager LS.
Cannot
focus
Q-View
Imager LS
Clicking the
focus buttons
has no eect
Disconnect the
Q-View Imager LS
USB cable from
the computer and
power cycle the
Q-View Imager LS.

13For Research Use Only
Appendix
Q-VIEW IMAGER LS
SPECIFICATIONS
Resolution: 18 Megapixels
Pixel Pitch: 4.11 microns
Sensor Type: CMOS
Sensor Size: 337.5mm2
Optics: EF-S 60mm f/2.8 Macro
USM Lens
Image Application: SBS plate format with
Chemiluminescent blots
(7.6 cm x 12.1 cm)
Imaging Time: 1-5 minutes
Control: Q-View Software
Connection to PC: USB
OPERATING SPECIFICATIONS
Operating Conditions: Temperature range of
15-35°C and dew point
of no greater than 20°C,
non-condensing
Power Requirements:
(includes ±10% of
nominal voltage)
110-120V/60 Hz/0.38A
Max
220-240V/50 Hz/0.2A
Max
Dimensions: 20.32 W x 25.4 D x
55.88 H cm (8 x 10 x 22
inches)
Weight: 9.12 kg (20.12 lbs)
Overvoltage Category: OVC II

Part Number
365 North 600 West, Logan, Utah 84321
Tel: [phone] | Fax: [phone] | www.quansysbio.com
For technical support, email: [email protected]