Endress+Hauser Analytik Jena Biometra TSC ThermoShaker User manual

Biometra TSC ThermoShaker
Order No. 846-051-600 (230 V)
Order No. 846-051-690 (115 V)
Manual
846-9-990-031
Please read these instructions carefully
before using this apparatus!
Biometra GmbH
Service Department
Rudolf-Wissell-Str. 30 Rudolf-Wissell-Str. 14-16
D-37079 Göttingen D-37079 Göttingen
Germany Germany
Phone: +49 5 51 / 50 68 6-0 Phone: [phone], -12, -14
Fax: +49 5 51 / 50 68 6-66 Fax.: +49 5 51 / 50 88 1-11
E-mail: [e-mail] e-mail: [e-mail]
Internet: http://www.analytik-jena.com

Manual Biometra TSC ThermoShaker, Vers. 01_2019
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1 Contents
1CONTENTS ....................................................................................................... 1
2SAFETY............................................................................................................. 2
2.1 DEFINITION OF SYMBOLS:.................................................................................. 2
2.2 SAFETY PRECAUTIONS ...................................................................................... 2
3SCOPE OF DELIVERY...................................................................................... 4
4ACCESSORIES................................................................................................. 5
5GENERAL INFORMATION ............................................................................... 6
6INSTALLATION................................................................................................. 7
6.1 UNPACK AND CHECK......................................................................................... 7
6.2 INSTALLATION................................................................................................... 7
6.3 INSTALLATION OF BLOCK MODULES................................................................... 7
6.4 CHANGING BLOCK MODULES............................................................................. 8
7OPERATION OF TSC THERMOSHAKER........................................................ 9
7.1 PARAMETER SETTING...................................................................................... 10
7.1.1 Reaction Time (TIME).......................................................................................10
7.1.2 Shaking Intensity (RPM)...................................................................................10
7.1.3 Reaction Temperature (T, ºC) .........................................................................10
7.1.4 Program Execution............................................................................................10
8CALIBRATION ................................................................................................ 12
8.1 CALIBRATION PROCEDURE............................................................................... 12
9MAINTENANCE............................................................................................... 14
9.1 CLEANING ...................................................................................................... 14
9.2 RUBBER BELT REPLACEMENT .......................................................................... 14
10 SPECIFICATIONS........................................................................................... 15
11 SERVICE ......................................................................................................... 17
11.1 DEVICE RETURN INFORMATION ......................................................................... 17
12 NOTE FOR DISPOSAL OF ELECTRIC/ELECTRONIC WASTE .................... 18
13 DECLARATION OF CONFORMITY................................................................ 19
Manual for apparatus with serial number 010143-1208-0106 or higher

Manual Biometra TSC ThermoShaker, Vers. 01_2019
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2 Safety
2.1 Definition of Symbols:
Caution: Read these operating instructions fully before use and pay
particular attention to sections containing this symbol.
Caution: Surfaces can become hot during use.
Caution: Danger! High voltage!
Caution: Fragile!
2.2 Safety Precautions
General Safety:
Use only as specified by the operating instructions, or the intrinsic protection may
be impaired.
The unit should be saved from shocks or drops.
The unit must be stored and transported in a horizontal position (see packaging
labelling).
After transport or storage in humid conditions, dry out the unit before connecting it
to the supply voltage. During drying out the intrinsic protection may be impaired.
The unit must be placed on a level, non-flammable surface away from flammable
materials.
Use appropriate vessels / tubes for temperature required. Ensure that the
operating temperature is less than the maximum operating temperature of your
sample material.
Clean the unit only with a damp cloth. Do not use chemical cleaning agents.
Before using any cleaning or decontamination method except those
recommended in this manual, users should check with the Biometra Service
Department that the proposed method will not damage the equipment.
Do not make modifications to the design of the unit.
The ThermoShaker TSC has to be used only with a power supply compatible with
the connection port and with similar electrical parameters like the included power
supply. It is highly recommended only to use this supplied power supply.

Manual Biometra TSC ThermoShaker, Vers. 01_2019
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Electrical Safety:
Connect only to a power supply with a voltage corresponding to that on the serial
number label at the rear of the unit.
The TSC Thermoshaker has to be used only with a power supply compatible with the
connection port and with similar electrical parameters like the included power supply.
It is highly recommended only to use this supplied power supply.
Ensure that the mains switch and isolating device (external power supply) are easily
accessible during use.
Do not plug the unit into the mains outlet without grounding, and do not use extension
lead without grounding.
Before moving, disconnect at the power supply socket.
To turn off the unit completely, disconnect the external power supply from the mains
outlet.
Never fill liquid directly into the blocks (always use vessels).
If liquid is spilt inside the unit, disconnect it from the power supply and have it
checked by a competent person.
Disconnect the mains before removing the outer cover. Note there are no user
serviceable parts inside in the unit. (Warranty is void if cover has been removed by
user!)
During Operation:
Do not leave the operating unit unattended.
Do not impede the platform motion during operation.
Do not operate the unit in environments with aggressive or explosive chemical
mixtures.
Do not operate the unit if it is faulty or been incorrectly installed.
For indoor use only.
Do not operate the unit outside the laboratory premises.
Do not check the temperature by touch. Use a thermometer.
Do not touch surfaces which become hot during high temperature operation.
Do not put any hot blocks on inflammable surfaces.
To reduce the risk of eye injury during high temperature operation, use safety goggles
or spectacles.
Biological & Cchemical Safety:
It is the user's responsibility to carry out appropriate decontamination if hazardous
material is spilt on or inside the equipment.

Manual Biometra TSC ThermoShaker, Vers. 01_2019
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3 Scope of Delivery
The Biometra TSC ThermoShaker (Order No. 846-051-600/690) is delivered with
Fig. 1: Delivered parts
1 x ThermoShaker apparatus (Fig. 1/1)
1 x External power supply unit + cable (Fig. 1/2)
2 x Spare rubber belt (Fig. 1/3)
4 x Rubber spacers for block screws
1 x Manual
Interchangeable block modules (Fig. 2) have to be ordered separately and will
be delivered separately.
1
2
3

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4 Accessories
Interchangeable block-modules
Order No. Description
846-051-615 Interchangeable block module for TSC, 24 x 1.5 ml tubes
846-051-616 Interchangeable block module for TSC, 24 x 2.0 ml tubes
846-051-612 Interchangeable block module for TSC, 20 x 0.2 ml + 12 x 1.5 ml tubes
846-051-613 Interchangeable block module for TSC, 20 x 0.5 ml + 12 x 1.5 ml tubes
846-051-614 Interchangeable block module for 96 well microtiter plates or 96 x 0.2 ml tubes





846-051-615 24 x 1.5 ml tubes
846-051-616 24 x 2.0 ml tubes
846-051-612 20 x 0.2 ml + 12 x 1.5 ml tubes
846-051-613 20 x 0.5 ml + 12 x 1.5 ml tubes
846-051-614 96 well microtiter plates or 96 x 0.2 ml tubes
Fig. 2: Biometra TSC ThermoShaker and interchangeable block modules

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5 General Information
The TSC ThermoShaker is
a compact bench top
shaking incubator, ideal for
all applications requiring
heating, cooling and shaking
in microtubes up to 2.0 ml,
within the temperature range
of -15 °C below room
temperature (RT) to +100 °C.
The intensive mixing
operation and the
incubation/heating/cooling
mode can be used either
combined or independently
from each other, i.e. the
device can work as a
thermoshaker, incubator
without shaking/mixing or as
shaker/mixer without temperature control.
The TSC ThermoShaker is applicable for DNA and RNA analysis, extraction of lipids and
other cell components, DNA library creation, biochemical studies of enzymatic reactions and
processes, extraction of metabolites from cellular material, preparation of samples for
electrophoresis, etc.
The TSC ThermoShaker provides:
Peltier elements for active cooling
Variable speed, variable temperature micro tube thermoshaker
Gentle to vigorous mixing of samples (2 mm shaking orbit)
Soft start function
Even amplitude throughout the shaker platform
Timed shaking operation (1 min. to 96 hours) with buzzer and automatic
switch-off
Set parameters will be saved
Low voltage power supply (for safe cold room operation)

Manual Biometra TSC ThermoShaker, Vers. 01_2019
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6 Installation
6.1 Unpack and Check
Unpack and carefully examine the TSC ThermoShaker. Report any damage to Analytik
Jena. Save all packing material if damage is found.
Attention! Automatic balancing system in this product produces light metal-like
noise when moving the unit which is likely to be heard during unpacking. It is
normal occurrence and does not indicate to a fault!
Do not attempt to operate this device if physical damage is present.
If you would like to send the unit back to us, please read the return instructions (page 17).
6.2 Installation
Place the apparatus upon even horizontal non-flammable surface away from any flammable
material.
To provide optimum ventilation ensure clearance around the apparatus: 20 cm on each side.
Ensure additional clearance from the bottom side and do not place any object below the unit.
Plug the external power supply unit (AC/DC adapter supplied with the shaker) into the 12 V
socket at the rear side of the apparatus.
6.3 Installation of Block Modules
Attention: Installation and change of block modules have to be performed
only when the Power switch is turned off and the external power supply is
disconnected from the mains outlet.
Choose the block module.
Connect the plug to the contact terminal according to Fig. 3/1 on the bottom side of the block
module. Make sure that the connector is mounted tightly.
Fig. 3: Connecting block modules
Align the block module that the warning labels are facing the front of the unit.
Secure with the four knurled screws.
Installing the 96-well block module, drive one by one 4 screws for several turns and fix them
with the hexagonal key (included with the block module.
1

Manual Biometra TSC ThermoShaker, Vers. 01_2019
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6.4 Changing Block Modules
Turn the power switch to off and disconnect the external power supply from the mains outlet.
Remove the four knurled screws (Fig. 4/1) and disconnect the 10 way plug (Fig. 4/2).
Fig. 4: Changing block modules
Select the new block module.
Install the block module according to 6.3.
Attention:
Please handle the block modules carefully, do not let them drop.
Please disconnect and connect the 10 way plug carefully, do not damage it.
10 way plug

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7 Operation of TSC ThermoShaker
Before starting, please
check the tubes / microtiter plates before using.
be sure that tubes / microtiter plates are thermo-resistant.
don't heat the tubes / microtiter plates over the melting point of the material they are
made of (use thermo-resisting polypropylene tubes).
remember that thin-walls tubes have a higher thermo-conducting factor.
Under the action of high temperature (> 85°C) tube caps can open, thus causing sample
volume shrinkage or potential health risk when working with infected material. To prevent
such cases it is recommended to use tubes with cap lock of Safe-Lock®type.
For efficient mixing it is recommended to fill test tubes up to 75% of the rated volume.
Starting Operation:
Connect the power supply unit to the mains. Switch on by using the on-off switch at the rear
side of the TSC (Fig. 5).
Fig. 5: Rear side of the TSC
The upper line of the display (settings) shows time, RPM and temperature set earlier. (Fig. 6)
The lower line of the display (actuals) shows current readings of the same parameters
(STOP - time, 000 - RPM, thermoblock temperature °C, which automatically starts rising
according to the temperature set in the upper line). (Fig. 6)
Fig. 6: Control panel
1
2
3
4
5
On/off switch

Manual Biometra TSC ThermoShaker, Vers. 01_2019
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The time of temperature stabilisation depends on the initial temperature but does not exceed
15-20 min if the set temperature is 37.0 °C.
7.1 Parameter Setting
Use the readings in the upper line of the display (set point), while setting the necessary
parameters.
7.1.1 Reaction Time (TIME)
With the help of “▲” and “▼“ buttons (Fig. 6/1) set the required working time interval in hours
and minutes. If the button is pressed for longer time the increment becomes bigger
(1 min increments to reach 10, 20, 30, 40, 50 or 60 min, after this 10 min increments until
reaching the full hour and finally 1 hour increments).
7.1.2 Shaking Intensity (RPM)
With the help of “▲” and “▼“ buttons (Fig. 6/2) set the required shaking intensity in
revolutions per minute (increment 10 RPM). If the button is pressed for longer time the
increment becomes bigger (10 RPM for the first 10 steps, after these 100 RPM increments to
reach 1,400 RPM).
7.1.3 Reaction Temperature (T, ºC)
With the help of “▲” and “▼“ buttons (Fig. 6/3) set the necessary temperature (increment
0.1ºC). If the button is pressed for longer time the increment becomes bigger (0.1°C
increments to reach 1°C, 2°C, …., after this 1°C increments until reaching 10°C, 20°C,… and
finally 10°C increments to reach 100°C).
Notes:
The set parameters can also be changed during operation.
The block temperature is maintained independently of the set time and
shaking speed
7.1.4 Program Execution
After the TS1 ThermoShaker has reached thermal stabilisation (when the set and current
temperature readings become the same) insert tubes into the platform sockets of the
interchangeable block module.
Press the “Shaker”-Start/Stop button (Fig. 6/4). The platform will start rotation and the timer
indicator will start counting up the time interval (with 1 min precision).
Note:
If the rotation speed is set to zero, pressing “Shaker”-Start/Stop button starts
the timer but the platform does not move.

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At the end of the program (after the set time elapses) the platform motion stops and the timer
shows the flashing reading STOP accompanied by the repetitive sound signal until the
“Shaker”-Start/Stop button is pressed.
If the working time is not set (or deleted) and the timer indicator in the upper line shows
00:00, pressing the “Shaker”-Start/Stop button causes the apparatus to operate continuously
until the “Shaker”-Start/Stop button is pressed again.
If required, there is possibility to restart the timer when it is running. Press the “Timer”-
Start/Stop button once (Fig. 6/5) to stop the timer. Press the “Timer”-Start/Stop button again
to restart the timer.
The platform motion can be stopped at any time by pressing the “Shaker”-Start/Stop button.
In this case the program and the platform motion stop, the timer is set back to zero and
switches into the STOP mode. Press the “Shaker”-Start/Stop” button to repeat the operation
with the same time and speed.
Attention:
At the end of the set time period the platform movement is stopped
automatically, but the heating can be stopped only manually by reducing the
temperature with the “▼“ T(ºC) key (Fig. 6/3 - lower button) till the OFF sign
appears in the upper line of the display.
At the end of operation switch off the unit by using the on-off switch at the rear side of the
TS1 (Fig. 5).

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8 Calibration
The instrument is pre-calibrated at the factory (calibrating coefficient - 1.00) for operation with
measured temperature from sensor installed in the thermoblock.
To enter the calibration coefficient, hold TIMER-START/STOP key (Fig. 6/5) pressed for
more than 8 sec to activate calibration mode. Display will show the calibration coefficient as
shown on fig.8/1.
To restore the factory settings with the help of Temp. “▲” and “▼“ keys (Fig. 6/3) set 1.000
value as shown on fig.8/1.
To exit calibration mode press SHAKER-START/STOP key (Fig. 6/4) once.
8.1 Calibration procedure
To perform calibration install independent sensor (accuracy 0.5°C) inside tubes into the block
sockets or right in thermoblock special sensor socket.
Set the necessary temperature (e.g. 40°C).
After the unit reaches the set temperature (when the set and current temperature readings
becomes the same) it is necessary to leave unit for 30 min for thermal stabilization.
Let us assume that the readings of independent sensor is 39 °C, but the display’s actual
temperature is 40 °C (Fig.7), it is necessary to add correction 1 °C.
Fig.7 Control panel in operation mode
Hold TIMER-START/STOP key (Fig. 6/5) pressed for more than 8 sec. to activate calibration
mode. Display will show the following parameters as shown on fig.8.
Fig.8 Control panel in calibration mode
Actual p.
Set p.
set temperature
actual temperature
hr:min RPM T( C)
o
00:00 1000 40.0
STOP 0000 40.0
sct. 40.0 40.0
1.000 40.0 40.0
calibration
mode set
temp.
actual temp. multiplied by last stored
actual coefficient
calibration
coefficient
temperature
the internal sensor
from
temp. with
coeff.multiplier -

Manual Biometra TSC ThermoShaker, Vers. 01_2019
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Fig.9 Control panel in calibration and operation mode
To set the new temperature value, use the Temperature with multiplier - coefficient readings
(Fig.8/3).
With the help of Temp. “▲” and “▼“ keys (Fig.6/3) change the calibration coefficient
(Fig.9A/1) so, that the new temperature value (Fig.9A/2) corresponds to the independent
sensor temperature. In our example the calibration coefficient will be 0.974 (in range: 0.936
up to 1.063; increment 0.001).
This calibrating coefficient will correct temperature though all operation range.
After calibration press SHAKER-START/STOP key (Fig.3/4) once to save the changes and
exit calibration mode.
The display will show calibrated temperature as shown on fig.9B/3 and the unit will continue
thermal stabilization according to the previously set temperature.
calibrating
coefficient
sct. 40.0 40.0
0.974 39.0 40.0 00:00 40.01000
STOP 39.00000
temp. with
coeff.multiplier -
set temperature
calibrated temperature

Manual Biometra TSC ThermoShaker, Vers. 01_2019
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9 Maintenance
All products covered in this manual are designed to comply with IEC61010-1 and can be
flash tested. As they are fitted with radio frequency interference suppressers it is
recommended that only a D.C. test is performed.
No other routine service is required.
9.1 Cleaning
The case can be cleaned with a damp cloth after disconnection. Do not use liquids that
contain concentrate organic solvents, alkali or acid nor abrasives.
Standard ethanol (75%) can be used for cleaning and disinfection of the unit.
Attention:
Disconnect the unit from the power supply socket.
Before using any decontamination or cleaning method except that recommended, check with
our Service Department that the proposed method will not damage the equipment.
9.2 Rubber belt replacement
Dependend on the frequency and the mixing speed it might be necessary to regularly replace
the rubber belt. We recommend a replacement after a period of 1.5 years of operation
respectively after 2,000 hours of operation.
Fig. 6: Rubber belt replacement.
Remove the external power supply unit (AC/DC adapter supplied with the shaker)
from the mains and from the 12 V socket at the rear side of the apparatus.
Remove 4 fixation screws on the shaker bottom and remove the bottom plate.
Replace the rubber belt (Fig. 6).
Assemble the unit.

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10 Specifications
Temperature setting range +4 ºC to +100 °C
Temperature control range RT –15 °C to +100 °C
Temperature setting resolution 0.1 °C
Temperature stability ±0.1 ºC
Temperature accuracy at +37°C ±0.5 ºC
Temperature uniformity over the block
at +4 °C ±0.6 °C
at +37 °C ±0.1 ºC
at +100 °C ±1.0 ºC
Average heating rate (20 x 1.5 ml block) 5 °C/min
In the range from +25 °C to +100 °C
Average cooling rate (20 x 1.5 ml block)
- in the range from +100 °C to +25 °CRT 5 °C/min
(from +100 °C to +25 °C in 14 min)
- in the range from RT to 15 °C below +25 °C 1,8 °C/min
(from +25 °C to +4 °C in 12 min)
Time of block-module heating from
+25 °C to +37º C 6 min
15 min (average, including
temperature stabilisation)
Temperature calibration coefficient range 0,936 … 1,063 (± 0.063)
Speed range 250 -1,400 RPM
(increment 10 RPM)
Max. speed deviation
at 250 RPM 2%
at 1,400 RPM 0.7%
Orbit 2 mm
Independent timer with sound signal 1 min - 96 hrs
(increment 1 min)
Maximum continuous operation time max. 96 h
(recommended interval between operation sessions not less than 8 h)
Display 16 x 2 characters, LCD
Capacity of interchangeable block modules 24 x 1.5 ml tubes
24 x 2 ml tubes
20 x 0.5 ml + 12 x 1.5 ml tubes
20 x 0.2 ml + 12 x 1.5 ml tubes
96 x 0.2 ml or 96 well MTP

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Input current 12 V, 5 A
Power consumption 60 W
External power supply input AC 100 –240 V, 50/60 Hz
output DC 12 V
Dimensions (W x D x H) 205 x 230 x 130 mm
Weight (with power supply) 4.1 kg (at max.)
The TS1 ThermoShaker is designed for operation in closed laboratory rooms, cold rooms or
incubators at ambient temperature from +4 ºC to +40 ºC and maximum relative humidity of
80% for temperatures up to +31 °C decreasing linearly to 50% relative humidity at +40 °C.

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11 Service
Should you have any problems with this unit, please contact our service department or your
local Biometra dealer:
Analytik Jena/Biometra GmbH
Service Department
Rudolf-Wissell-Straße 14 - 16
D-37079 Göttingen
Germany
Phone: +49 5 51 / 50 88 1 - 10 -12 or -14
Fax: +49 5 51 / 50 88 1 - 11
E-mail: [email protected]
If you would like to send the unit back to us, please read the following instructions.
11.1 Device return information
WARNING
Risk of damage to health due to improper decontamination! Perform a
professional decontamination before returning the device to Analytik Jena
AG.
NOTICE
Biometra GmbH must refuse acceptance of contaminated devices. The
sender may be liable for any damage caused by inadequate
decontamination of the device.
Please clean all device components from biologically hazardous, chemical or radioactive
contamination (see also section "Cleaning”).
Download the decontamination declaration as an editable PDF document in German or
English from the Internet:
https://www.analytik-
jena.com/fileadmin/content/service/customer/Declaration_of_decontamination_en_01.pdf.
Complete the form and attach the signed decontamination declaration to the outside of the
shipment.
Only use the original packaging for the shipment and insert the transport lock.
If the original packaging is no longer available, please contact Biometra GmbH or your
local dealer.
Please attach the warning note
"CAUTION! SENSITIVE ELECTRONIC DEVICE!" to the packaging.
Please include a sheet containing the following data:
Name and address of the sender
Name and telephone number of a contact for inquiries
A detailed description of the fault, the precise conditions and situations under which
the fault occurs

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12 Note for Disposal of Electric/Electronic Waste
This symbol (the crossed-out wheelie bin) means, that this product should be
brought to the return systems and/or separate systems available to end-users
according to yours country regulations, when this product has reached the end
of its lifetime!
For details, please contact your local distributor!
This symbol applies only to the countries within the EEA*.
*EEA = European Economics Area, comprising all EU-members plus Norway, Iceland
and Liechtenstein.

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13 Declaration of Conformity
Biometra GmbH
Rudolf-Wissell-Str. 30
37079 Göttingen
Telefon [phone]
Telefax [phone]
E-Mail [email protected]
Göttingen, den 22.11.2018
Biometra erklärt als Hersteller in alleiniger Verantwortung, dass die Produkte
Biometra declares as manufacturer under sole responsibility that the products
Typen
Types:
Biometra TSC ThermoShaker
Biometra TS1 ThermoShaker
Best.-Nr.
Order No.:
846-051-600, 846-051-690
846-051-500, 846-051-590
den folgenden Europäischen Richtlinien und angewandten harmonisierten Normen entsprechen:
conform to the following European Directives and applied harmonized standards:
Richtlinie
Directive
Norm
Standard
Ausgabejahr
Year of Publication
2014/35/EU
EN 61010-1
2010
Niederspannungsrichtlinie/LVD
EN 61010-2-10
2014
EN 61010-2-051
2015
2014/30/EU
EN 61326-1
2013
EMV/EMC
EN 55011
2009
2011/65/EU
EN 50581
2012
RoHS
Dr. Juergen Otte
Head of Quality Management
For and behalf of Biometra GmbH