Rankin Basics CRY85 User manual

OPERATOR MANUAL
Rankin Basics Cryostat
CRY85
Attention: Before using the instrument, please read this Instruction carefully, and keep the Instruction for further
reference.
Please refer to the nameplate on the back of the instrument for the serial number and manufacturing date of the
instrument

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Table of Contents
1. Overview···················································································3
2. Technical Specifications ·······························································4
3. Installation and Working Conditions ················································5
4. Operation ··················································································6
4.1 Central operation panel··························································6
Left: Programming ON/OFF switch ······································6
Left: Fast defrost·······························································6
Middle: Peltier freezing·······················································7
Middle: Standby································································7
Middle: Sectioning/trimming setting·······································7
Right: Clock setting ··························································8
Right: Temperature setting ·················································8
Right: Fast freezing ··························································8
Right: Start-of-work timer ···················································9
Right: End-of-work timer ····················································9
Right: Defrost timer ··························································9
4.2 Side operation panel ··························································10
UV sterilization ······························································10
Cryochamber illumination ················································10
Slow feeding ·································································10
Fast feeding ·································································10
4.3 Identification and Operation of Mechanical Parts ······················· 11

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Blade holder ································································· 11
Changing blade ·····························································11
Adjusting the blade angle ················································11
Fit and adjust the anti-roll plate ········································· 11
Handwheel ···································································12
Specimen setup ····························································12
Sectioning procedures ····················································14
Tips for Successful Sectioning ···········································14
Recommended Temperature Ranges···································15
5. Troubleshooting ········································································16
6. Cleaning and Maintenance ··························································17
Cleaning the display screens·············································17
Cleaning procedures························································17
Empty the waste liquid bottle ·············································17
Repair and maintenance···················································17
7. Transportation ··········································································18
8. Safety Information ·····································································18
9. Accessory List ··········································································20
10. Limited Warranty ·····································································21

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1. OVERVIEW
This model has an ergonomic design and is manufactured using numerically - controlled
machine tools. It is recommended for use in hospitals, medical schools, scientific research institutes,
and food quarantine and inspection organizations.
With its novel design, this instrument has a high precision, stable performance, and easy
operation. It possesses the following features:
▪A specimen retraction function protects the specimen from blade damage.
▪A trim button allows for easy switching between trim and sectioning mode.
▪It has a counting function for the number of sections and the total thickness.
▪The cryogenic refrigeration system utilizes a forced cooling structure. The refrigeration
chamber, freezing shelf, blade holder, and specimen holder are cooled independently by a
German double compressor to enhance cooling performance and increase cooling speed.
▪Imported R404A non-CFC refrigerants that are environment-friendly.
▪It achieves the desirable sectioning temperature within 60 minutes after turning on the unit
when using the specimen head cooling.
▪UV option enables surface disinfection of harmful bacteria, viruses, and spores by using 35
min after each use.
▪The Peltier semiconductor cooling function on the quick freeze shelf can be turned on and off.
▪Heated and removable glass door.
▪Two defrosting modes: programmed defrosting and manual defrosting.
▪Microtome is outside of the refrigeration chamber to avoid the effect of heat-expansion and
cold-contraction, thus minimizing the need for maintenance.
▪User-friendly intelligent operational interface is easy to learn and operate.
▪LCD screen displays the number of sections and the total thickness, section thickness,
specimen retraction value, temperature setting, date, time, temperature, and on/off timer are
all included on the main display.
▪Auto sleep function results in longer compressor life: the cryochamber temperature
automatically stays in a range of -4 to -9℃under the sleep mode, and returns to the sectioning
temperature within 10 minutes after canceling the sleep mode.
▪Keyboard locking function to avoid accident mis-operation.
▪A mechanical handwheel lock ensure safe chamber access.
▪A large quick-freezing shelf allows for the preparation of 26 specimens at the same time, two
of which are cooled by a Peltier semiconductor.

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2. TECHNICAL SPECIFICATIONS
Section thickness range: Adjustable range 1-100μm
1 –20 μm Increment: 1 μm;
20 –40 μm Increment: 2 μm;
40 –100 μm Increment: 5 μm;
Trimming thickness range: Adjustable range 10-400 μm
10-50 μm Increment: 5 μm;
50-100 μm Increment: 10 μm;
100-400 μm Increment: 50 μm;
Specimen retraction: Adjustable range 0-80 μm Increment: 5 μm
Horizontal stroke: 20 mm
Vertical stroke: 60 mm
Voltage: AC 110V±10% / AC 220V±10%
Frequency: 50~60Hz
Power: 600W
Largest start-up current（5 second）45A
Chamber temperature: -10℃to - 40℃
Freezing shelf temperature: -10℃to - 45℃
Number of freezing stations: 26
Pelletier number: 2
Refrigerant: R404a,300±10g
Compressor oil: 0.6L EMKARATE RL-22S, ICI
Working noise: 52dB(A)
Dimension size(mm): 650 x 805 x 1160
Net Weight: 240 Lbs.

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3. INSTALLATION AND WORKING CONDITIONS
Package handling
•Please inspect the package and contact the shipper if the package has serious damage.
•Please place the instrument on a stable surface after taking it out from the wooden box.
•Do not place the instrument in an environment near explosive or flammable material.
•If the instrument may need to be transported again, we suggest using the original packaging
materials.
Installation
•The instrument is a movable floor type with 4 caster wheels. Two front wheels can be adjusted
up and down by turning the knobs on the foot, which should be used only when the instrument
needs to be moved. After the package is opened, put the front wheels on the ground and
move it to the working location; lock the front wheels to secure the instrument.
•Avoid obstruction around the instrument and leave a space at least 12 in wide on both sides
as well as the back of the instrument to facilitate ventilation and heat dispersion.
•The instrument can be used after it is kept standing still for at least 2 hours.
•Please ensure the grounding wire of the 3-line power is securely connected before starting the
instrument, which must comply with the GB9706.1 standard. Please ensure the socket is
stable before connecting it and turning on the power switch.
•Insert the supplied power cord into the power socket and then connect the power cord to the
mains supply.
•Install the blade holder if necessary.
•Close the heated window.
•Turn on the power switch.
•Program all settings if necessary.
•For the first time use, the default setting of the blade holder and specimen head temperature
are -15°C.
•The cooling phase will take approximately 1 to 1.5 hours depending on the preset
cryochamber temperature.
No other instruments should be connected to the circuit used for the cryostat, as the
compressor needs high surge currents when started. Do not use multi-socket power
outlets with small wire sizes for the supply of the instrument.

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Working environmental conditions
● Temperature: +5℃to +28℃
An ambient temperature below 26℃is conducive to a longer lifetime of the cooling
compressor
●Humidity: < 80%
4. OPERATION
4.1 Central Operation Panel (Fig 1)
Fig 1
After the power source is connected to the rear of the cryostat, turn on the power switch
at the rear to allow it to enter the working mode. The feeding arm will go back to the original
position and the compressor starts running to cool down the freezing chamber.
Note: To protect the instrument from being damaged by an abrupt startup, the instrument
cannot be re-started within 3 minutes after it is turned off.
The central operation panel includes three zones: left, middle and right.
Left zone
The left zone is to control software and fast defrost.
Programming ON/OFF switch
Press this button to turn on or off on the entire operation panel. When the indicator light
is on, user may set up the instrument, such as adjusting on/off timer, defrost schedule,
and temperature setting.
Fast defrost
Press this button to activate fast defrost function and put the system into a fast defrost
High temperature or humidity will affect refrigeration performance.

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mode, as indicated by the indicator light. Fast defrost takes about 6 minutes. Press again
to stop fast defrost, and meanwhile the indicator light goes off. Freezing recovers after 25
seconds. In the case where the system automatically enters the defrost mode and the indicator
lights is on, user may press this button to stop the defrost process.
Middle zone
The middle zone is for section/trimming settings, controlling the Peltier, and the standby
mode.
Peltier freezing (not available under the standby mode)
Press this Peltier switch button to run the Peltier element on the quick freeze shelf, as
indicated by the indicator light. The 4th line on the screen shows the remaining cooling
time of the Peltier, e.g., [15] indicates 15 minutes, which will count down to [14], [13] …[2], [1]
until the Peltier stops cooling and the indicator light is off. User may also press this button to
stop the Peltier when it is running. The Peltier stops running automatically if the temperature of
the cooled storage is not below -10℃.
Standby
In the standby mode, the cryochamber remains at a temperature of -4℃to -9℃. This mode is
recommended if the instrument won’t be in use for a period time, which may prolong the
lifetime of the compressor.
Press the standby button to turn on/off the standby mode, which is indicated by the
bright or dim LCD screen, respectively.
Section/trimming setting
When the system is turned on, the LCD screen is as below:
→Section thickness
→Retraction thickness
→Number of sections
→Total thickness
Press this mode selection button to select between sectioning or trimming.
Press this button to clear the displayed values of total number of sections and
thickness into “0”.

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When both M and C buttons are pressed together, one “*”signal appears in front of “Retr”
to indicate that user can now press “+”or “-“to adjust the retraction value.
“+”or “-“is to increase or decrease the values for sectioning/trimming, or
retraction (when “*”appears in front of Retr).
Right zone
This zone is for time and temperature setting. The two red LED lights above indicate the working
status of the big compressor (left) and the smaller Peltier compressor (right). The left
light is on when the compressor starts to work, and the right light turns on when
pressing to activate fast freezing.
After the cryostat turns on, it automatically enters the initial mode as below:
→Current weekday (1-7 indicates Monday to Sunday)
→Current time (00:00 - 23:59)
→T0=Chamber T2 = Spec. head (actual temp. [set temp.])
→T1 = Quick freeze shelf temp. (actual temp. [Peltier on/off]
Clock setting
The clock can be set by pressing “D” to adjust weekday and pressing ”H” and “M” to adjust hour
and minute, respectively.
Temperature setting
Press the temperature button, the third line on the LCD screen will shift between T0
(Chamber temperature) and T2 (Specimen head temperature).
These two buttons are to increase or decrease the temperature value for
cryochamber (T0) or specimen head (T2). See Page 16 for optimal temperature
settings per tissue type.
Fast freezing (invalid under standby mode)
This cryostat has two compressors: one large compressor for cryochamber, and one small
compressor for the storage area and specimen head. The small compressor does not run if the
system is in the standby mode. Fast freezing requires both compressors to run and can be
activated only when the system is under the normal working mode. Once fast freezing is
activated, the small compressor starts in 3 minutes to cool the storage area and clamp.
Day 1
Time 12:00
T0 -21 [-24]
T1 -45 [off]

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Fast freezing can be stopped by the user, or automatically if the Peltier begins to freeze over.
Press this button to activate/inactivate fast freezing, which is indicated by the right LED
light and the running sound of both compressors.
Programming start-of-work timer
Press this timer setting button until the second line on the LED screen shows “TON”,
then use the “D” ”H”, and “M” buttons to set the start-of-work time.
User can set single-day start-of-work time as following:
After pressing the “W-DAY”button, the first line in the LCD will show “Dwork 1&6”(
“1&6”represents Monday to Saturday). Press the “D”button to select a working day,
then press the ”H” and “M” buttons to adjust hour and minute, respectively. The system
will automatically set 24 hours of the standby mode for non-working days.
Programming end-of-work timer
Press this timer setting button until the second line on the LED screen shows “TOff”,
then use the “D” ”H”, and “M” buttons to set the end-of-work time. After setting, press
this timer setting button again to store the settings and return the LED screen to the
initial mode.
Programming defrost timer
Press this defrost timer button, the first line on the LCD screen displays “Defrost”and
the second line displays “Time”. User may press the ”H” and “M” buttons to adjust hour
and minute. If defrost is not needed, please set “hours”to 24. Press this defrost timer
button again to store the settings and return the LED screen to the initial mode.