RBCBioscience MagCore Plus II User manual

MagCore® Plus II
Operation Manual
Ver. 2019-2
www.rbcbioscience.com
CONTENTS
Safety Precautions . . . . . . . . . . . . . . . . . . . . . 1
Specification . . . . . . . . . . . . . . . . . . . . . . . . . . 2
━ Operating Parameters . . . . . . . . . . . . . . . . . . . . . . 2
━ Operating Environment . . . . . . . . . . . . . . . . . . . . . 2
━ Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
━ Before Installation . . . . . . . . . . . . . . . . . . . . . . . . . 3
━ System Overview . . . . . . . . . . . . . . . . . . . . . . . . . 4
━ Install Reagent Cartridge . . . . . . . . . . . . . . . . . . . . . 5
━ Install Tube, Tip . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
━ Installation Settings And Test . . . . . . . . . . . . . . . . . . . 6
Touch Panel and User Interface . . . . . . . . . . . 7
Operation Before Starting the Instrument . . . . . . . . . . . . 8
━ Emergency Stop . . . . . . . . . . . . . . . . . . . . . . . . . . 9
━ Start Programs . . . . . . . . . . . . . . . . . . . . . . . . . . 10
━ Saved Settings . . . . . . . . . . . . . . . . . . . . . . . . . . 11
━ Access to Saved Settings . . . . . . . . . . . . . . . . . . . . 11
━ Set and Select Users . . . . . . . . . . . . . . . . . . . . . . . 12
━ Set User . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
━ Select User Name . . . . . . . . . . . . . . . . . . . . . . . . 12
━ Scan Sample / Elution tube . . . . . . . . . . . . . . . . . . 13
━ Scan Kit Lot . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
━ Pause and Abort . . . . . . . . . . . . . . . . . . . . . . . . . 14
━ Set up the System Time . . . . . . . . . . . . . . . . . . . . 14
━ Set the Instrument Nickname . . . . . . . . . . . . . . . . . 15
━ Network Connection Settings . . . . . . . . . . . . . . . . . 15
━ LIMS Data Collection . . . . . . . . . . . . . . . . . . . . . . 16
Get Data from USB Flash Drive . . . . . . . . . . . . . . . . . 16
Access to Information by Internet Connection . . . . . . . . 16
Easy Maintenance . . . . . . . . . . . . . . . . . . . 17
━ Replace O-Ring . . . . . . . . . . . . . . . . . . . . . . . .17
━ Piston Test . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
━ Clean Piercing Needles . . . . . . . . . . . . . . . . . . . .20
━ Eject Tip . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
━ Heater Test . . . . . . . . . . . . . . . . . . . . . . . . . . .21
━ UV Sterilization . . . . . . . . . . . . . . . . . . . . . .21
━ Alarm History . . . . . . . . . . . . . . . . . . . . . . . . . .22
━ System Status . . . . . . . . . . . . . . . . . . . . . . . . . .22
━ Program Update . . . . . . . . . . . . . . . . . . . . . . .23
━ Clean the Waste Box . . . . . . . . . . . . . . . . . . . . .23
━ Replace Fuse . . . . . . . . . . . . . . . . . . . . . . . . . .24

1www.rbcbioscience.com
Handle the power cord carefully.
Hold the plug when unplugging the cord.
Keep the set free from moisture,
liquid, and dust.
Unplug the power cord if the system
will not be used again in a long time.
Do not obstruct the ventilation space.
Do not let foreign objects in the set.
Do not let insecticides, benzene, and
thinner come in contact with the set.
Do not ever disassemble or modify
the set in any way.
Avoid high temperatures.
Leave space for sufficient heat dissipation during installation.
CAUTIONS
Risk Of Electric Shock
Do Not Open
Safety Precautions
Before Use
WARNINGS and CAUTIONS stated below are intended to protect the individual from injuries
and avoid damage to machines.
CAUTION:
To reduce the risk of electric shock, do not remove the cover. Refer to qualified
service technician for inner part servicing.
CAUTION:
1. Handle The Power Supply Cord Carefully
Do not damage or twist the power supply cord. Damaged or twisted cord may
cause electric shock or error when used. When you remove it from the outlet, be
sure to remove by holding the plug and not by pulling the cord.
2. Do Not Place Anything Inside
Do not place metal objects inside the MagCore®System and wipe away all spilled
liquid to minimize the risk of system error and electric shock.
NOTE ON USE:
1. Read Instructions – All the safety and operating instructions should be read before
operating the instrument.
2. Keep the Instructions – Please keep the safety and operating instruction manual for
future reference.
3. Cautions and Warnings – All warning signs should be attached on the product and
shown in the user's manual.
4. Follow Instructions – All operating and use instructions should be followed.
5. Cleaning – Unplug the product before cleaning. Use only 75% of EtOH to clean the
surface of instrument.
6. Attachments – Do not attach anything on the instrument.
7. Liquid and Moisture – Do not use this product near water and avoid moist areas -
bath tub, wash bowl, kitchen sink, laundry, swimming pool or in a moist basement.
8. Proper Installation – Do not place this product on an unstable cart, stand, tripod,
bracket, or table. The product may fall and caues serious injury to a child or adult, and
serious damage to the product. Use only with a cart, stand, tripod, bracket, or table
recommended by the manufacturer, or sold with the product. Any mounting of the
product should follow the manufacturer’s instructions, and should use a mounting
tool recommended by the manufacturer.
9. Ventilation – Slots and openings in the cabinet are designed for ventilation, which
is required for safe operation and minimizing the chance of overheating. The product
should be placed on a hard surface to ensure proper ventilation. This product should
not be placed in a built-in installation such as a bookcase or rack unless proper
ventilation is ensured or instructed by the manufacturer.
10. Power Sources – This product should be operated only from the type of power
source indicated on the label. If you are not sure of the type of power supply at your
lab, consult the seller or local power company. For products intended to operate from
battery or other sources, refer to the operating instructions.
11. Grounding or Polarization – This product may be equipped with a polarized
alternating-current plug. This plug will fit into the power outlet in one way only. This is
a safety feature. If you are unable to insert the plug fully into the outlet, try reversing the
plug. If the plug should still fail to fit, contact your electrician to replace your obsolete
outlet. Do not defeat the safety purpose of the polarized plug.
12. Power-Cord Protection – Power-supply cords should be routed so that they are
not likely to be walked on or pinched by items placed near or against them. Pay extra
attention to the plugs, convenience receptacles, and the connecting point where the
cord extends out of the product.
13. Lightning – Unplug the product if it will not be used again for a long period or in the
case of a heavy storm. This will prevent damage to the product due to lightning and
power surges.
14. Overloading – Do not overload wall outlets, extension cords, or integral convenience
receptacles as this can result in a risk of fire or electric shock.
15. Object and Liquid Entry – Never push objects of any kind into this product as they
may touch dangerous voltage points or short-out parts that could result in a fire or
electric shock. Never spill liquid of any kind on the product.
16. Servicing – Do not attempt to repair this product by yourself. Refer all servicing to
qualified service technician.
17. Damage Requiring Service – Unplug this product from the wall outlet and refer
servicing to qualified service technician under the following conditions:
a) When the power-supply cord or plug is damaged,
b) If liquid has been spilled, or objects have fallen into the product,
c) If the product has been exposed to rain or water,
d) If the product does not operate normally following the operating instructions.
Adjust only controls covered by the operating instructions as an improper adjustment
of other controls may result in damage and will often require extensive work by a
qualified technician to restore the product to its normal operation,
e) If the product has been dropped or damaged in any way, and
f)When the product exhibits a distinct change in performance
– this indicates a need for service.
18. Replacement Parts – When replacement is required, be sure the service technician
uses replacement parts specified by the manufacturer or those with similar features
as the original part. Unauthorized substitutes may result in fire, electric shock, or other
hazards.
19. Safety Check – Upon completion of any shipment, storage, installation or
maintenance service to this product, ask the service technician perform safety checks
to confirm that the products is in proper operating condition.
20. Heat – The product should be situated away from heat sources such as radiators, heat
registers, stoves, or other products that produce heat.
21. Moving Product - The machine requires two people lifting from both side at the
bottom edge. Do not attempt to lift by bending forward. Bend your hips and knees to
squat down, keep the machine close to your body, and straighten your legs to lift.
General Warning. Please follow the instruction for operation,
be aware of electrical hazard caused by inappropriate
operation.
1. Power Supply
2. Barcode Scanner
Warning: Biological Hazard
Warning: High Temperature
Please read the instruction carefully and keep for future
reference.
This product is confirmed with European Union conformity
assessment regulations regarding health and safety or
environmental protection.
Waning: Electricity
SAFETY INSTRUCTIONS

2
MagCore® Plus II
Model Plus II
System Method Cellulose coated magnetic beads
System Components 1. Pipetting Unit: Dispensing, transferring, X -Y two axis movements.
2. Electric Control: PLC module and Arm-based main board embedded in.
3. UV Light: Power 8W, life duration 11,000 hrs .
4. Heating Block: RT-90° C .
5. Display Screen: 7 inch colored touch panel .
6. Accessories: T - Rack, Cartridge Rack s, barcode scanner
Power Supply Voltage: AC 100V~240V; Frequency: 50/60Hz ; Max. 4.5AMax, 450W
Dimension W523 x D602 x H605 (mm) / W20.59x D23.7 x H23.81 (inches)
Net Weight 70kg / 154.35lbs
Specification
━ Operating Parameters
━ Operating Environment
━ Applications
Applications: Whole Blood DNA (200l/400l/1,200l), Viral Nucleic Acid (DNA/RNA 200l/400l/1,200l), FFPE Genomic DNA,
FFPE Total RNA, Forensic DNA, Plasma and Circulating Cell-Free DNA (1,200l/4,000µl), Tissue Genomic DNA, Plant Genomic
DNA, Bacterial DNA, Whole Blood total RNA, Cultured Cells total RNA, Tissue total RNA, Plant total RNA.
Processing Capacity 1-16 samples per batch
Processing Time 30-90 minutes (depends on sample type and method)
Sample Volume 200 l /400 l /1,200 l / 4ml
Elution Volume 30l /40l /60l /100l /150l /200 l
Yield Average 6g Genomic DNA from
200l human whole blood
Purity DNA : O.D A260/280 ratio 1.8 ± 0.1
RNA : O.D A260/280 ratio 2.0 ± 0.2
Pipetting Accuracy 500l ≤ 4%
Humidity Allowed During Operation: 30%-75%
Pressure Allowed During Operation: 700hPa-1060hPa
Temperatures Allowed During Transportation. Storage Packaging 15°C-35°C
Temperatures Allowed During Operation 10°C-40°C
Pollution Degree Level 2

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Accessories
Installation
1. T-Rack X 1
2. Cartridge Rack X 1
3. Power Cable X 2 (Europe standard and U.S.A standard)
4. Grease X 1
5. 3mm O-Rings X 16
6. 5mm O-Rings X 16
7. Empty Cartridge X 10
8. Sample Tube /ElutionTube X 20
9. PipetteTip X 20
10. Waste Box X 1
11. Barcode Scanner X 1
Please refer to the machine size shown in the above figure to prepare an appropriate position for the machine and
reserve space for thermal dissipation.
12345
7
6
89
10 11
━ Before Installation
605 mm
Left Side (Keep space clear)
Height (Keep space clear)
602mm
523mm
Depth
Width

4
MagCore® Plus II
━ System Overview
Touch Panel
Logo Indicator
Front Door
Back Service Lid
Operation Area
Waste Box
Scanner Port
USB Port
Main Power Switch
Ethernet Port
Power Outlet

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━ Install Tube, Tip
Warning :
Please do not use the Tips and Tubes which are not provided by the original manufacturer.
The test result may be not correct and the machine may be damaged due to different Tips
and Tubes.
━ Install Reagent Cartridge
1. Put the tip into the corresponding well
according to the left figure.
2. Put the T-Rack on the machine.
Please install the Tip and Tube according to
the instructions of extraction kit user manual.
Please insert the Cartridge Rack before
the T-Rack.
Please insert the front end of the cartridge into the
space below the fixing plate of the Cartridge Rack.
W5 Elution Tube
W1
W2
W3
W4
W5
W4 200μl SP Tip
W3 Pipette Tip
W2 DNase / Tip Holder set
W1 Sample Tube - FS Tip

6
MagCore® Plus II
━ Installation Settings And Test
Step 1.
Please Set up the Local Time.
Please refer to System Status chapter for the information
about the operation.
Step 2.
Set the Instrument Nickname
Go to Page 13
Step 3.
Network Connection Settings
Go to Page 15
Step 4.
Test Piston
Please refer to Piston Test chapter for information
about the operation.
Step 5.
Test Heater
Please refer to Heater Test chapter for information
about the operation.
SystemTime Setup
Month
Day
Minute
Current Time
Year Hour
59
01
01
2014 23
2014/01/01 23:59
CancelUpdate

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Touch Panel and User Interface
MagCore® Plus II provides 7-inch full-color touch panel and easy-to-use user interface. Operator
only needs to pay attention to the panel to know the current status, ending time and test report of
the machine. The above figure shows the function list of the operation screen and the description
of the status icons.
Start
Report
Setup
Saved Protocols
Maintenance
Eject Tip
Execute Extraction Execute quick run with
selection of pre-saved
protocols
System Maintenance
Functions
Overview of the latest
runs with barcodes
System Settings
Status Column Icons Panel Board Lighting
Front Door is Open
Front Door is Closed
Machine Connected to Network
Machine is not Connected to
Network
USB Flash Drive Plugged In
* Format USB Flash Drive to FAT32
The Panel will light up the following lights when the
instrument is in operation:
Blue Light: The Instrument is on Stand-by
Green Light: The Instrument is in Operation
Red Light: The Instrument Gives a Warning Alert

8
MagCore® Plus II
Operation Before Starting the Instrument
Close the door after the setting is completed and
press START to run the extraction.
Please Follow the Instructions Below to Operate the Instrument:
After loading the reagents, cartridges and tips, see page 5 and set the required parameters without
opening the door.
After closing the door, press START to start operation.
Plunged Upward from
the Lower Edge of the
Front Door
The Front Door Will
Rise Automatically For the sake of safety, the front door will not open to the top.
Please push the front door up to
raise it to the top when needed.
Precautions When Opening the Front Door
A.
B.
BA

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━ Emergency Stop
Please read the security information to correctly stop the machine
when an accident takes place.
When an emergency situation occurs and the machine is
running, please press the stop button or open the door of
the machine to stop the machine immediately.
Pause
Current Status

10
MagCore® Plus II
Select DNase Treatment. (Only for RNA kit.)
Select Sample Volume.
Select Elution Buffer. (Only for tissue kit.)
Close door and press Start.
Please pretreat the sample according to the
instructions of the user manual of the MagCore®
Kit and put consumables into the machine.
Press Start to go to the next step.
To Select User and Scan Barcodes: See Page 12
Press next to choose the code number.
Please confirm the reagent set code number to be
used in the extraction.
Select the number of the cartridge.
Select User
Select
Scan Sample Scan Elution Tube
Scan Kit LOT
Back Next
Jemes
01/31/2019 13:15:47
━ Start Programs
13:1
5:47
13:1
47
!The number
can be found in the
sticker on the cartridge.
If you need to save this setting, please
refer to page 11 to know how to save
customized settings.

11 www.rbcbioscience.com
If you need to save a customized setting, you can
save it here and use the predetermined parameters
for later uses.
After selecting the parameters, the confirmation
of your settings will be displayed. Press SAVE AS to
open the Saved Protocols page.
Select the parameters that you want to save shown
in the blue area. Saved Protocol option saves up to
six records.
Press Save Protocol to save the customized
parameters.
Press Back to confirm your settings.
After loading the Tips and Cartridges:
Press Saved Protocols on the start page
Select the parameters that you want to execute
shown in the blue area.
Press Start Protocol
Press Start after confirmation of your selected
parameters.
━ Saved Settings ━ Access to Saved Settings

12
MagCore® Plus II
Instrument can help you record the User Name as
well as the Sample, Elution Tubes and Kit Lot No.
used in the extraction for LIMS integration data.
Select this icon if you want to add or edit an user´s
information. Instrument provides up to six records.
Edit name on the name block as shown in the
figure.
Press Enter when finish editing.
Press the name block you want to select
After displaying of the selected name, press Done
to finish.
━ Set and Select Users
━ Set User ━ Select User Name
Select User
Select
Scan Sample Scan Elution Tube
Scan Kit LOT
Back Next
Jemes
01/31/2019 13:15:47
Select User
Select
Scan Sample Scan Elution Tube
Scan Kit LOT
Back Next
Jemes
01/31/2019 13:15:47
It
A S D F G H J K L
Z X C V B N M
Q W E R T Y U I O P
Enter BackspaceEsc
!@# $ % ^ & * ( ) .
,
JAMES
er
(
e
(

13 www.rbcbioscience.com
Select Scan Kit LOT
The system will ask you if you want to delete all
the barcode data stored before. Press Execute to
delete it
Deletion Completed
You can start scanning, and the system will
automatically jump to the next item after scanning.
If you need to rescan an item, please press the
digital block to rescan.
━ Scan Sample / Elution tube ━ Scan Kit Lot
Select User
Select
Scan Sample Scan Elution Tube
Scan Kit LOT
Back Next
Jemes
01/31/2019 13:15:47
Select User
Select
Scan Sample Scan Elution Tube
Scan Kit LOT
Back Next
Jemes
01/31/2019 13:15:47
Select Scan Sample or Scan Elution tube
The system will ask you if you want to delete all
the barcode data stored before. Press Execute to
delete it.
Deletion Completed
You can start scanning, and the system will
automatically jump to the next item after scanning.
If you need to rescan an item, please press the
digital block to rescan.
There is a kit LOT barcode
on the Cartridge Box label
of the KIT you purchased.
You can scan this barcode
for LIMS data storage
purposes.

14
MagCore® Plus II
━ Pause and Abort
You can pause or abort the current extraction
while the program is in progress. Press the Pause
button to pause at any time, and the machine will
immediately suspend operation. This button is
used as an emergency stop button.
After the Pause screen appears, you can press
Continue to continue with the extraction or press
Abort to cancel all.
Aborting operation in progress
Abortion Completed
━ Set up the System Time
Select System Time Setup to set up the system
time of the machine.
Please input the local date and time and then press
the Update button to save the system time. You
can see the time that you changed in “Current
Time” after the change is finished.
Setup
Back
01/31/2019 13:15:47
System Time Network InfoUnit nickname System Status
Ba
m T
Ba
m T

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You can give the instrument an easy-to-remember
nickname for remote APP identification.
Select Setup Button
Select Unit Nickname
Select the blue square as shown in the figure
above.
Enter the name you want to give to the instrument
and then press Enter to finish adding or changing
the name.
The network connection is used for the remote
monitoring APP and LIMS data transmission.
To set the remote monitoring app, you need
to first to check up with your IT staff that your
network environment has DHCP service and can
connect to the Internet and open the firewall 8083
(out), 1883 (out), 8883 (out), 80 (out) , 443 (out)
port.
When using LIMS data transfer, you need to
first establish a 21 (in) connection with the IT
staff to determine the firewall of your PC. When
transmitting as a LIMS data, we operate on the
internal network.
After setting the network environment to meet
the above rules, please insert the network route
into the network port of the instrument and boot
it.
After the connection is made, the network
will automatically assign one IP location to
the instrument. We must make sure that the
instrument is assigned to one IP
Press Setup Button
Select Network Info
Please confirm the displayed TCP/IP location and
DHCP server. The same network segment is sent
to complete the network settings.
━ Set the Instrument Nickname ━ Network Connection Settings
It
A S D F G H J K L
Z X C V B N M
Q W E R T Y U I O P
Enter BackspaceEsc
!@# $ % ^ & * ( ) .
,
JAMES
Ethernet Port
Remote Monitoring APP
Server Connection Status
Regional Network
Connection Status

16
MagCore® Plus II
There are two ways to get the data. 1. Get it via
USB Flash Drive.
Get Data from USB Flash Drive
Plug the USB Flash Drive into the instrument USB
Port.
Select Report
Select Save Data to save the data to the USB Flash
Drive. * Format USB Flash Drive to FAT32
Access to Information by Internet
Connection
Obtaining data via internet connection. We
provide FTP protocol as a means of data
transmission. You can use any FTP client software.
Get the information FTP location information is as
follows:
Ftp:// instrument IP location (eg 192.168.3.155)
Username: uploadhis
Password: 111111
Open the Internet browser and enter the FTP
location and enter the username.
Select and download the data list and passwords.
━ LIMS Data Collection
USB Port

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━ Replace O-Ring ━ Piston Test
Easy Maintenance
Easy Maintenance includes the maintenance items
which need to be done by users. These items include
sterilization, clearing, simple test and error message
report, etc. Users must perform these checkups
periodically to ensure the system status is always
good.
Please select Replace O-Ring button.
1. Please disassemble the damaged O-Ring.
2. Replace a new O-Ring.
3. Greasing the O-Ring, and perform the Piston
Test.
The Piston test includes the tests for three Tip
types, which mainly test the tightness of Piston
O-Ring and Tip, and the smooth of taking the
Piston from the T-Rack.
Please select Piston Test button.
Maintenance:
Please perform the Piston Test andgrease the
O-rings once a month.

18
MagCore® Plus II
A. Test the Tip of the W1.
1. Please put the FS Tip into the W1 of T-Rack.
2. Please add 1 ml water to the Elution Tube and
put it in the W5 of the T-Rack.
3. The test can be divided into two stages. The first
stage is to observe the smoothness of taking the
tip out of the Tip Holder. Please check whether
the tip holder is taken out together with the tip.
If the Tip Holder is taken out together with the
tip, please reset the Tip Holder and try it again.
4. Please check whether the liquid levels of all tips
are consistent with each other. If the liquid levels
of all tips are not consistent with each other,
please check the O-Ring (Please refer to the
Replace O-Ring chapter for more information).
B. Test the Tip of the W2.
1. Please put the Tip Holder Set (not provided in
accessary) into the W2 of T-Rack.
2. Please add 1 ml water to the Sample Tube and
put it in the W1 of the T-Rack.
3. Please select the Test W2 Tip.
4. The test can be divided into two stages. The first
stage is to observe the smooth of taking the tip
out of the Tip Holder. Please check whether the
tip holder is taken out together with the tip. If
the Tip Holder is taken out together with the tip,
please reset the Tip Holder and try it again.
5. Please check whether the liquid levels of all tips
are consistent with each other. If the liquid levels
of all tips are not consistent with each other,
please check the O-Ring (Please refer to the
Replace O-Ring chapter for more information).

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C. Test the Tip of the W3
1. Please put the Pipette Tip into Well 3 of T-Rack.
2. Please add 1 ml water to the Sample Tube and
put it in the Well1 of the T-Rack.
3. Please select the Test W3 Tip.
4. Please check whether the liquid levels in all tips
are consistent with each other. If the liquid levels
are not consistent, please check the O-Ring
(Please refer to the Replace O-Ring chapter for
more information).
D. Test the Tip of the W4
1. Please put the 200μl SP Tip into Well 4 of T-Rack.
2. 2. Please add 1 ml water to the Sample Tube
and put it in Well1 of the T-Rack.
3. Please select the Test W4 Tip.
4. Please check whether the liquid levels in all tips
are consistent with each other. If the liquid levels
are not consistent, please check the O-Ring
(Please refer to the Replace O-Ring chapter for
more information).