RWD D180P User manual

CO2Incubator
User Manual A

© 2019 Shenzhen RWD Life Science Co., Ltd,All rights reserved.
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The intellectual property rights of this product and its instruction manual belong to RWD Life
Science Co., Ltd (hereinafter referred to as the RWD), including but not limited to patent,
trademark, copyright etc..
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RWD have the right to use the instruction as confidential information. Any individual and
organization shall not disclose the instruction of all or parts of the information by any means
without RWD’s written permission. Nor shall any other person or organization be allowed to
obtain all or part of the information of this instruction manual by any means.
No individual and organization shall but not limited to publish, modify, reproduce, issue, rental,
adapted, and translated into other languages with RWD’s written permission.
is RWD’s registered trademark or mark, these trademarks and the related security
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Statement
RWD reserves the right to modify the content of this manual without prior notice.
RWD reserves the right to change the technology without prior notice.
RWD reserves the right to modify the product specifications without prior notice.
RWD is not to guarantee the information in any forms, including (but not limited to) the guarantee
responsibility of proposing the implied merchantability and suitability for a specific purpose.
RWD only in the following conditions is considered to be responsible for the safety, reliability and
performance of the instruments, i.e.:
Assembly operation, expansion, adjustment, improvements and repairs were carried out by RWD
authorized personnel;
Relevant electrical equipments in line with national standards;
The instrument is operated according to the instruction manual.
RWD is not responsible for the products’ safety, reliability and operation status in the following
conditions:
Components are disassembled, stretched and debugged;
Non RWD authorized personnel repairs or alterations to the instruments;
Product using not in accordance with the manual.

Contents
1-Introduction ...................................................................................................... 1
1.1 Overview.......................................................................................................................... 1
1.2 Safety............................................................................................................................... 1
1.3 Comprehensive description............................................................................................. 1
1.4 Product features.............................................................................................................. 2
1.5 Equipment environmental requirements........................................................................ 2
1.6 Specification .................................................................................................................... 2
1.7 Settable parameters........................................................................................................ 3
1.8 Product list....................................................................................................................... 3
2-System safety .................................................................................................... 4
2.1 Safety symbols................................................................................................................. 4
2.2 Safety instructions........................................................................................................... 5
2.3 Precautions for operation ............................................................................................... 5
3. Product assembly and structure ........................................................................ 7
3.1 Incubator Installation ...................................................................................................... 7
3.1.1 Precautions before Installiation ............................................................................... 7
3.1.2 Preliminary cleaning ................................................................................................. 7
3.1.3 Install the inner cover assembly............................................................................... 7
3.1.4 Install vertical plates and ejector rob assembly....................................................... 8
3.1.5 Install the horizontal shelve ..................................................................................... 9
3.1.6 Install the access port filter .................................................................................... 10
3.1.7 Installing the HEPA filter......................................................................................... 10
3.1.8 Adjust the equipment level .................................................................................... 10
3.1.9 Connecting the Unit to Electrical Power ................................................................ 10
3.1.10 Filling of humidity pan .......................................................................................... 11
3.1.11 Connect to CO2gas supply ................................................................................... 11
3.1.12 Reverse Door Swing.............................................................................................. 12
3.1.13 Stacking incubators .............................................................................................. 14

3.2 Product show and ports introduction ........................................................................... 16
4-Operation Instruction .......................................................................................18
4.1 Initial start ..................................................................................................................... 18
4.2 Menu ............................................................................................................................. 18
4.3 Temperature and CO2concentration settings .............................................................. 19
4.4 Alarm settings................................................................................................................ 20
4.5 High temperature sterilization ...................................................................................... 21
4.6 Sensor calibration.......................................................................................................... 23
4.7 General .......................................................................................................................... 24
4.8 Other operations ........................................................................................................... 24
4.9 Alarm and prompt message .......................................................................................... 26
5-Maintenance ....................................................................................................29
5.1 Preventive maintenance................................................................................................ 29
5.2 Clean the inside of incubator ........................................................................................ 30
5.3 Clean Cabinet Exterior................................................................................................... 30
5.4 Clean the inner door (glass door) .................................................................................. 30
5.5 Clean the humidity pan ................................................................................................. 31
5.6 HEPA filter maintenance ............................................................................................... 31
5.7 Replace the fuse ............................................................................................................ 31
6-Troubleshooting ...............................................................................................32
7-Product warranty..............................................................................................33

1-Introduction
1.1 Overview
First of all, sincerely thanks for selecting the CO2 Incubator manufactured by RWD.
Please read this user manual and all other auxiliary materials carefully before
installing and using the product, it will be helpful for you to work better.
RWD has been always dedicated to improving the product function and the service
quality, and will reserve the rights to revise the products itself and contents described
in the instruction manual at any time without notice in advance.
If you find the practical situations about the supplied goods do not agree with the
contents described in the manual, or have any questions or ideas about our products
and service, welcome to contact us. For latest information, please visit our web site
(http://www.rwdstco.com/) or contact us immediately.
The User Manual applies to CO2Incubator below:
CO2Incubator—D180-P
1.2 Safety
Before operating the system, please read the “2-System Safety” section
carefully to avoid damages to laboratory animals, operators and the system during
application.
If you have any question or suggestion regarding safety, please contact RWD
for after-sales support.
1.3 Comprehensive description
The CO2Incubator is an experiment box product designed, developed and
produced by RWD, and also an advanced type of equipment for cell, tissue and
bacteria culture. The CO2Incubator ensures stable internal temperature, stable
carbon dioxide level, constant pH, and high saturated humidity by the precise
control systems for temperature, carbon dioxide and humidity, that to make the
normal growth of cells.
This product is commonly used in fields such as microbial culture,
cytodynamics research, collection of mammalian cell secretions, carcinogenic or
toxicological effects of various physical and chemical factors, antigen research and
This equipment should be operated and managed by
trained professionals!

production, culture of hybridoma cells for antibody production, in vitro fertilization
(IVF), stem cells, tissue engineering, and drug screening.
1.4 Product features
Triple sterilization function. Continuous sterilization for 24 hours,
professional HEPA filtration system, and irregular sterilization;
Efficient, fast and easy high temperature sterilization cycle ensures not only
effective disinfection and sterilization, but also automatic running at night.
The entire sterilization cycle is less than 3 hours, and the total cycle is about
14 hours;
Temperature homogeneity. Thermoelements are located on the outer wall of
the incubator and is directly heated via six sides to ensure superior
temperature uniformity and reduce the time of heating sterilization.
The microprocessor control system is used to monitor the whole process of
high temperature sterilization in real time, and also display the status
information in different stages of high temperature sterilization cycle in real
time.
1.5 Equipment environmental requirements
The equipment operating environment is prepared according to the conditions
listed below to ensure the operability and safety of the system.
Detailed description
Working environment
Temperature: 5°C - 40°C
Humidity: 15%-95% (non-condensing)
Air pressure: 103.4 kPa
Storage environment
Temperature: -20°C - 60°C
Humidity: 10%-95% (non-condensing)
Air pressure: 50kPa-106kPa
Operating voltage 1) 210V-230V VAC 50/60HZ, 5A
2) 100V-120V VAC 50/60HZ, 10A
1.6 Specification
Parameters
Detailed description
Screen size 7-inch capacitive touch screen
External dimension:
Length × width × height 67cm*64.7cm*97cm
Internal dimension: Length
54.7cm*51cm*67.5cm

× width × height:
Capacity ~180L
Weight ~120kg
Heating method Inner wall heating, air casing
1.7 Settable parameters
Parameters Detailed description
Temperature 10.0-50.0°C
CO2 concentration 0.0-20.0%
Temperature alarm threshold
Upper limit: (T+0.1) - (T+5.0)
Lower limit: (T-0.1) - (T-5.0)
CO2 concentration alarm threshold Lower limit: (C-0.1) - (C-5.0)
Upper limit: (C+0.1) - (C+1.0)
Lower limit of humidity alarm Lower limit: 80-100%
HEPA filter screen countdown Adjustable range: 0 - 365 day(s)
1.8 Product list
Attachment Accessory name Quantity Application description
Standard Host 1 Body of CO2Incubator
Standard
Vertical plate of CO2
Incubator (provided
with 6 button holes)
2 For supporting stainless steel shelves
Standard Stainless steel shelves 3
Carrier for culture dishes (up to 15
optional shelves, each with load
bearing of 7kg)
Standard 5.5 inch HEPA filter 1 Air filtering
Standard Humidity pan 1 Holding water to increase humidity
Standard Components of stacking 4 For stacking and fixing of equipment
Standard Access port filter 2 For air filtering
Standard Ejector rob assembly 3 For supporting the vertical plate of
CO
2
Incubator

2-System safety
Please read the safety instructions carefully. For safety’s sake, please be aware
of the following:
Correct connection cables
Make sure all connection cables are safely and securely connected to the
equipment.
Avoidance of all exposed electronic cables
Do not touch any electronics and wiring inside the equipment!
Shutdown due to suspected failure
In the case of equipment safety hazard or failure of normal operation,
please contact the authorized technical support personnel.
Correct connection of equipment
Connect the equipment correctly to avoid operation difficulty or even
disconnection of equipment due to incorrect connection.
2.1 Safety symbols
The following safety symbols and general marks may be used in the User
Manual and on the equipment. If you have any question or suggestion regarding
safety, please contact RWD for after-sales support. To prevent damages to the
equipment and extend the service life of the equipment, please follow these
instructions carefully.
- General safety warning.
Operate the device only in accordance with this user manual. Follow
these general precautions during operation.
- High temperature warning.
Hot surfaces can burn unprotected skin or heat can damage materials.
- Risk of electric shock.
To avoid electric shock, the device must be properly connected to the
protective ground wire.
-Waste treatment. Separate taking back of electrical and electronic
instruments in countries of the European Union: this is applicable in
countries of the European Union and other European countries with a

separate collecting system for electronic waste. This product must be
disposed of within the waste management regulations.
- Warning!
Parts and components that are susceptible to electrostatic discharge
(ESD) are included
–
Asphyxiation Hazard Warning.
High concentrations of CO2 can displace oxygen and cause
asphyxiation!
–
Equipment being maintained or serviced must be turned off and
locked off to prevent possible injury.
2.2 Safety instructions
Plug in the power cord to prevent improper power contact;
Pay attention to shockproof, waterproof, moisture proof, pressure proof and
fireproof measures;
During moving and handling equipment, pay attention to handling strength to
prevent damages to equipment or falling of equipment;
The CO2Incubator should be managed by specific personnel. It is strictly
forbidden for untrained personnel to operate the equipment. Please use a
reliable grounding device.
The responsibility for equipment failure arising from any improper cleaning,
maintenance and operation of the equipment should be borne by the user.
If the equipment host is disassembled without the authorization of RWD, RWD
will no longer fulfill its commitments for quality assurance and technical
maintenance services to the equipment. For any technical problems please
contact the authorized personnel or RWD for supports.
2.3 Precautions for operation
The CO2Incubator should be regularly cleaned and disinfected with
disinfectant. Its stainless steel shelves can be removed for cleaning and
disinfection to avoid failure of the experiment due to contamination by other
microorganisms.
If no carbon dioxide will be used for a long time, the CO2cylinder should be
closed to prevent the CO2regulator from malfunctioning.
The purity of used CO2must be 99.5%, or otherwise the sensitivity of CO2
sensor will be reduced and the CO2filter will also be contaminated.
The inner door of the CO2Incubator cannot be condensated. Any observed

water mist should be wiped out.
The pressure value of carbon dioxide inlet should between 1- 2 atmospheres.
(1Standard atmosphere =101.325kPa=1bar=14.5Psi）).
If the CO2Incubator is overheated at 160°C, the internal temperature protector
will cut off the heating power. In this case, it is necessary to restart the power
supply after 8-12 hours of power-off.

3. Product assembly and structure
3.1 Incubator Installation
3.1.1 Precautions before Installiation
1) At least 10 cm of clearance should be left behind the CO2Incubator to
allow the connection of electrical or gas supply devices to the equipment.
In addition, at least 10 cm of ventilation clearance should be left on each
side of the equipment.
2) The equipment should be mounted on a solid horizontal surface capable of
supporting a weight of 120 kg.
3) The equipment should be placed away from doors, windows, heaters and
air conditioner ducts.
4) Lift the CO2Incubator along both sides of its base instead of lifting it from
its front and rear sides.
3.1.2 Preliminary cleaning
1) Any plastic film observed on the horizontal shelves, vertical plates, and
ejector rob assembly should be removed.
2) Clean all internal surfaces with suitable laboratory disinfectants.
3.1.3 Install the inner cover assembly
1) Install the inner cover assembly on the erection column on the top of the inner
container (note that the air duct direction needs to be facing inside), and lock the inner
cover with butterfly nuts from the accessory kit.
2) Connect the other end of the air duct to the carbon dioxide sampling port, and
ensure that the connection is correct and airtight.
Erection column
Inner cover assembly
Butterfly nut

3.1.4 Install vertical plates and ejector rob assembly
1) Attach the provided buttonholes to the flanges on the rear side of each
vertical plate (each plate is provided with 6 button holes).
2) Install two vertical plates with buttonholes into the CO2Incubator, and
ensure that the hanging piece is toward the center of the chamber with the
hanging slot facing up. The installation direction of vertical plates need not
to be distinguished, any one of them can be installed on the opposite side
by rotated. During the installation process, tilt vertical plates slightly so
that they can be easily loaded, and then adjust them to the vertical position.
3) Install the vertical plates on the both sides, and then install the bracket for
horizontal shelf. The rear hole of the horizontal shelf bracket is hung on the
Carbon dioxide sampling
port
Air duct
Button hole (3 on left and 3 on right)
Vertical plate
Enlarged
view
Hanging slot
Opening
upwards

corresponding hanging piece of the vertical plate, and the position can be
adjusted as needed. Pull the horizontal shelf bracket forward so that its
front hole engages with the corresponding front hanging piece of the side
vertical plate.
4) Ejector rob assembly. Find three ejector robs, springs and end covers from
the accessory kit (2 pieces for the rear part of inner container and 1 piece
for the upper front part of inner container). Insert spring ends from ridges
into ejector robs and press the springs against the opposite side of the
ejector robs. Spring will then be inserted into place after a cracking sound.
Slide the end covers into ejector rods from above the springs. Insert one
end of ejector rod into the corresponding hole of the vertical plate, and then
the installation is completed.
Note: To install an ejector rod assembly, do not install the assembly to the lower
edge of incubator near the humidity pan, or otherwise the pan may not be easily
replaced.
3.1.5 Install the horizontal shelve
Install the horizontal shelf into corresponding bracket for horizontal shelf as
shown below.
Vertical plate
Horizontal shelf bracket
End cover
Spring
Ejector rod
The opening of horizontal
shelf is facing down
Hanging slot

3.1.6 Install the access port filter
1) Find an access port in the upper left corner of the inner side of incubator
chamber.
2) Find the plug with filter from the accessory kit.
3) Push the opening cover to the right in the incubator and insert the filter
assembly into the opening.
3.1.7 Installing the HEPA filter
1) Take out a filter from the packaging box.
2) Remove the plastic sleeve from the filter, and do not touch the filter media.
3) Install the filter as shown below.
Note: Treat the filter carefully. Improper handling may damage the filter media.
To avoid damages to the incubator, do not operate the equipment if no HEPA
filter is installed.
3.1.8 Adjust the equipment level
Place a bubble level on the horizontal shelf to check whether the equipment is
level. Operation method: Rotate the hex nut of bubble level counterclockwise to
extend the frame, or turn it clockwise to shorten the frame. Level the equipment in the
front and rear, and left and right directions.
3.1.9 Connecting the Unit to Electrical Power
Note:
1) Make sure that the circuit can carry the current for sterilization cycle.
2) Connect the CO2Incubator to a grounded and dedicated circuit.
3) Please place the CO2Incubator reasonably so that the power supply can be
easily disconnected.
4) Insert the attached power cord into the power outlet on the back of the CO2
Incubator, and then insert the other end into a grounded and dedicated
circuit.
HEPA fixture
HEPA filter

3.1.10 Filling of humidity pan
1) Add sterile distilled water to the humidity pan until its water level is no more
than 2/3 of total distance from the top of humidity pan. Place the humidity
pan directly in the center of the bottom of the CO2Incubator.
2) If a high humidity is required, the humidity pan should be placed close to the
left side wall of the CO2Incubator, and then the CO2sampling port should
be closed to obtain the optimum humidity. However, this may also cause
condensation in the incubator chamber. To accelerate the recovery of
humidity after opening the door, a humidity pan should be placed in the right
tube.
3) In order to achieve optimal operating conditions for the CO2Incubator,
sterile distilled water, desalinated water or DI water should be used in the
humidity pan. The purity of water should be in the resistance range from
50K to 1M Ohm/cm, or in the conductivity range from 20.0 to 1.0 uS/cm.
4) Distillation systems and certain types of reverse osmosis and purification
systems can produce the water in a specified quality range. Tap water is not
recommended because it may contain chlorine and erode stainless steel. Tap
water may also contain large amounts of minerals that form scale in the
humidity pan. High purity water or ultrapure water is not recommended
because they are extremely corrosive solvents that can corrode stainless
steel.
5) The resistance of high purity water is from 1M to 18M Ohm. Even high
purity water may contain bacteria and organic pollutants. To ensure its safe
use for stainless steel materials and products, water should always be
sterilized and decontaminated before being poured into a humidity pan.
Note:
The resistance of distilled or DI water used in humidity pans must be in the
range from 50K to 1M Ohm/cm to protect stainless steel parts and extend
their service life. Using water outside the specified range will reduce the
service life of the equipment and may also void the warranty.
Check the water level regularly and change the water frequently to avoid
water contamination. Maintain a stable water level to avoid drastic
fluctuations in the water level.
3.1.11 Connect to CO2gas supply
Warning:
High concentrations of CO2 gas can cause asphyxiation! OSHA Standards
specify that employee exposure to carbon dioxide in any eight hour shift of

a 40-hour work week shall not exceed the eight-hour time weighted
average of 5000 PPM (0.5% CO2).. The short-term exposure limit less
than 15 minutes is 30,000 ppm (3% CO2). In confined areas, it is
recommended to monitor carbon dioxide level due to carbon dioxide
accumulation.
Only CO2applies to this series of incubators. Introduction of flammable or
toxic gases can cause dangerous conditions. Do not introduce gases other
than CO2to the equipment.
The CO2cylinder connected to the incubator should be of industrial grade
with a purity of 99.5%.
Do not use any CO2cylinder with siphons. A siphon is designed to draw
CO2liquid from the cylinder, which can damage the pressure regulator.
Please consult your gas supplier to ensure that the CO2cylinder is not
equipped with any siphon.
The cylinder should be fixed to a wall or other fixed object to prevent it
from falling over.
A CO2twin-stage pressure regulator must be installed on the cylinder
outlet valve. The input pressure of the incubator is suggested to be
maintained between 1.0bar and 1.5bar to ensure the proper operation of the
CO2control system. When the pressure in the CO2cylinder falls, The CO2
single-stage pressure regulator cannot maintain the pressure in the input
incubator at 1.0bar-1.5bar, so a two-stage regulator is suggested.
If CO2of higher purity (above 99.5%) is required in the incubator, a
stainless steel shelf should be installed on the pressure regulator and the
CO2of specified purity should be purchased from the gas supplier, and the
pressure regulator should also be correctly and safely connected to the
cylinder.
3.1.12 Reverse Door Swing
If side-by-side operations or changes to laboratory layout are necessary, you can
change the rotation direction of inside and outside doors in chamber. The
following steps describe how to change the rotation direction of chamber doors
from left to right. In order to facilitate the change to rotation direction of chamber
doors, screw holes have been prepared on the case. Necessary tools include a
cross screwdriver and a normal screwdriver. The entire operation process takes
about 30 minutes.
Operation steps:
1) Disconnect the power supply and ensure that the dead equipment is
operated as below (important).

2) Find 3 set screws at the back of the top cover and use a cross screwdriver
to remove them, and then retain these screws.
3) Push the top cover back from the front side and remove the cover.
4) Pull out the communication interface and front door heating film
terminal at the positions shown below.
5) Use a cross screwdriver to remove the screws on the small cover and
take out the wire plug and wire.
6) Use a screwdriver to remove 3 screws on the cover and change the outlet
hole to the left side.
Combined cross screws
Top cover
Pull out the
communication
interface
Screw
Small cover
Rubber plug

7) Use a screwdriver to remove 8 nuts on one side, remove the hinge and
fasten these nuts and hinge securely on another direction.
8) Use screws to secure the wire cover, small cover and top cover to their
original positions.
3.1.13 Stacking incubators
Warning: Stacking incubators must be installed against a wall (or similar
structure).
Warning: For stacking incubators, do not open the outside doors of two
incubators at the same time.
Warning: If the equipment is already running, turn them off and disconnect the
power before maintenance.
Warning: The weight of incubator is 120 kg. Therefore, make sure that there
are enough people to help lift the equipment. Lift the CO2Incubator
along both sides of its bas.
Four stacking racks are included in the accessory kit for each incubator
1) Stack two incubators and tighten the four supporting feet of the incubator
located above in order to ensure the stacking balance.
2) Use a cross screwdriver to remove the four fixing screws on the lower left
and lower right of the incubator above.
3) Remove the two fixing screws on the top left and top right of the incubator
below.
4) Remove the fixing screws on the left and right sides of the back of the top
cover.
5) Finally, the left and right and the rear sides of the two incubators were
connected and fixed respectively with the cross screw and stacking racks
of M5*12 in the incubator accessory package.
6) The completed sketch map is as follows.
Screw
Wire cover

Stacking rack
Supporting feet

3.2 Product show and ports introduction
Product real shot display
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