Olympus BHA-P User manual

I
OLYMPUS POLARIZING MICROSCOPE
MODELS
BHA
-P&B;H.-P
ATTACHMENT

i
This
instruction rnancral has been written for the use
of
the Olyn~pusPolarizing Microscope
!,
Modcl
CHA-P
and Polarizirl!j Attach~nentModel
131-I-P.
It
is
recommended
to
r-ead
the
I
>
manual carefully in order to familiarize yourself fully
with
the use
of
Ihe microscope on the
!
polarizing attachment so
lhal
you
can
ob~an
the
best
performance
and
~ffectiveness.
t
IMPORTANT
Observe
the
following points carefully:
Operation
1.
Always
handle
the microscope
with
the
care
it
deserves, and avoid abrupt motions.
2.
Avoid exposureof
the
microscope
to
direct sunlight.
dust
and vibration.
3.
Only
use
the
tension adjustment ringfor altering the tension
of
the coarse adjustment.
Do
not twist the two coarse adjustment
knobs
in
the
opposite directions sirnultane-
ously, which
might
cause damage.
4.
Ascertain that
the
voltage selector switch on the base plate is set
to
conformwit11
the
local mainsvoltage.
5.
Disconnect
the
line cord frorn
the
AC
power outlet for fuse replacement.
Maintenance
1.
Lenses
must
always
be
kept
clean. Fine dust
on
lens
surfaces
should
be
blown or
wiped off by means
of
an air blower
or
a
clean
brush.
Carefi~llywipe off oil
or
finger-
prints deposited
on
the
lens
surfaces
with
yaclzs moistened
with
a
small amount
of
xylene, alcohol or ether.
2.
Do
not use organic solutions
lo
wipe
the surfaces of various components.
Plastic
parts, especially,should
be
cleaned with
a
neutral detergent.
3.
Never disassemble the rnicroscope for repair
4.
The microscope should
be
stored
in
its container immediately aftel- use. li this
is
not
possible, it slioi~ld
be
covered with
ti
vinyl dust cover.
It
is best
to
kcap
objectives and
eyepieces in
a
desiccator, containingcjcsiccants.
5.
Disconnect the linecord
frorn
the
AC
power
soirrce before
fuse
replacement.

CONTENTS
t.
STANDARD
EQUIPMENT
. . .
.
.
. . .
.
.
.
. .
.
.
.
.
.
.
it.
NOMENCLATURE
.
.
.
.
.
. .
.
.
.
.
.
.
.
.
.
.
.
.
.
I1
I. ASSEMBLY
.
.
.
.
. .
.
.
.
.
.
.
.
.
. .
. . . . .
.
.
.
1V.
IDENTIFICATIONAND FUNCTION OF VARIOUS
COMPONENTS
.
. .
V. OPERATION
.
.
.
.
.
.
.
. .
.
.
.
.
.
.
.
.
.
.
.
.
.
. .
1.
ElectricSystem
2,
lnterpupillary Distance
and
Diopter
Adjustments
.
.
.
.
.
.
.
.
.
.
3.
Light Path Selection
4.
Centering
the Condenser
.
.
. .
.
.
.
.
.
.
.
.
5.
Centering
the
Stage
.
.
.
,
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
6.
Centering
the
Objectives
7.
Use of
Iris
Diaphragms
8.
Focusing Adjustment
.
.
.
.
.
.
.
.
.
. .
.
.
.
.
.
.
.
.
9.
OrthoscopicObservation
10.
Conoscopic Observation
.
.
.
.
.
. .
.
.
.
.
,
.
.
.
.
.
.
11.
Photomicrography
VI. OPTICAL
DATA
.
.
.
.
.
.
. .
.
.
.
.
.
.
.
.
.
.
.
.
.
.
VII. TROUBLESHOOTING.
.
.
.
.
.t
.
.
.
.
.
.
.
.
.
.
. .
.

-
1.
STANDARD
EQUIPMENT
Optional
Accessories:
Mechanical
stage
for
polarizing
use Model
AH-FMP
Berek
cornpensalor
Modsl
AH-CTP
Qhjectivc
(srrain-free)
P020X
Model
Microscope
stand
wrth
aux~lrary
lens
BHA-F
Rcvo
lving
nosepiece
RH-PRE
l~itermediate
polarizing
a~tachmenl
AH-PA
Quarter
wave
plate
AH-TPI47
BHA-751-P
J
1
1
BHA-651-P
1
1
1
1
pp
BH-P-1
I
0
1
I
1
Sensrt~ve
tint
plate
(53OrnpCI)
AH-TP530
Polarlztng
b~necular
tube
(30~)
BH-PB
130
Polari~lnq
tr~nocular
tube
(30~1
BH-PTH30
Circular
rotatable
stage
BH-SRP
Swing-out
condenser
BH
-POC
Tungsten
lamp
house
BH-LH
30-watt
tungsten
filament
bulbs
LS30
Objecrrves
(strain-free)
BH-P-2
0
1
1
1
1
0
1
1
1
3
1
1
PO4
X
POIOX
P040X
PO1
00X
Iollj
0
1
1
111
1
3
1
'
1
1
1
K5X
(with
cross
hatrs)
1
1-1
1
7
WFlOX
(with
cross
ha~rs}
1
1
1
1
Eyepieces
WF1
OX
(with
1011
00mm
micrometer)
1
BIKSX
1
1
1
1
WFlOX
1
1
11
1
1
1
1
1
1
1
Photo
~yep~ece
F
K5X
0
0
Spare
fuses
F~lter
45C
Immersion
nil
I
V~nyt
dust
cover
-
1
1
0
0
0
0
-
2
1
1
1
1
10
-
2
0
I
1
I0
1
0
'
,!
0
1
\
1
1
0
1
1
1
1
t
1
1
0
0
0
0

II.
NOMENCLATURE
Base
1

Ill.
ASSEMBLY
Tfic
141ctl1re
i~clow11111~t~atCsthe
scq~leritidl
~prncodr~r
1%
ol
assernbly
T!ie
nt~nhrvc
~ndtcrlt~
the
assembly
cjrrlnr
ol
vilrlous
colnponenls. Rel~iove
dust
caps
before mounting
Lurnpunents.
Take
cars
lo
Ireep
all
glaqs
surfaces cleat),
and
avalcl
si.4
al~4t111g
tlie
srrr
faces.
0
Eyepiece
Insert
the
objective
10X
inlo
the
fixed
aperture
of
the
nose-
piece.
$1
Objective
"I
@
Qbservat~on
tu11e
Clamping
screw
Quarter
wave
plate
Sensitive
rint
@j
lntermedrate Dolarizing
tube
plate
Attach
the
tube
to
the
stand
--
wrth
rhe
letters
"OLYMPUS"
Berek
facing
in front
of
the
micro-
compensator
scope
(or
align
the
red
dots).
(TI
Mechanical
stage
I
0
Nosepiece
\
k---
Rotatable
stage
M~croscops
stand
@
Lamp
house
Insert
the
two
pins
01
the
aux~liary
lens ~nto
the
bush.
rng5 on
the
microscoae
stand
unt~l
the
lens
clicks
into
posi-
tion.
w~th
the
convex
surface
(with
engraving
"UPS1DE")
tac~ng
upward.
Align~ng
red dots,
on
condens-
er
mount
and
condenser,
Insert
the
condenser
inro
the
mount
Prior
to
mounting
the
stage.
rack
down
the
condenser
mount
all
the
way.
To
rernwe
the
stage,
(*move
rhe
cbn-
denser
firs[.
Insert
the
lamp
house
and
pres-
sing
the clamping
screw
at
the
bottom
of
the
base,
rotate
he
lamp
house
until
the
locating
hole
and
clamplng
screw
are
al~sned.
,
Electr~c
connection
1)
Pluq 111
11i(!
t:o~hnr?ct~ng
cord
of
th~
lari~p
hocrsc
10
~hc
r~ccptactr:
at
tho
back
of
the
bds~
2)
Inscr
i
onc
plz~g
ol
the
linc
cord
to
the
Illis
cord
socket
on
rhe
llasc
and
coiincct
the
orher
or lug
to
rhe
AC
[lnwer
outlet.

;
)
IV. IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS
L~ght
path
selector
kr~ol)
/
Pull out the selector knob all
the way for photomicrography.
Analyicl- scaic
Tension adjustment ring
Arrow mark ~ndicatesIncrease
in coarse adjustment tension.
Voltmeter
\
SIlcIinq cont~~ollever
For cont~nuouslyvariable light
intensity.
\
L~icvol~~g~
selector
switch

Mer.lianical tube length
adjust-
Clarnping screw for
mechanical stage
ngraved letters "IN" for in-
sertion
of
Bertrand lens into
the light path;
"OUT"
for
removal of the Bertrand lens
from the light
path.
45'
click stop lever
Clamping screw for
stage
uated.
Filter mount
Polarizer scale
Reading to
5'.
Field iris diaphragm ring
Polarirer rotation ring
Rheostat trimmer screw
screwdriver until the
voltmeter
indicates IV,
with the slid~ng control lever positioned
closest to vou (low voltage).
Larnp house clamping screw

Nosepiece
clamping
Automatic
the cap
of
the fuse holder, and
replace the defective fuse with
a
replacement fuse.
(Disconnect BEFORE RE-
PLACEMENT.)

A. Match the linevoltage selector switch to local mains voltage (seepage
6).
Summary of Putting the Microscope in Operation
B. Switch on the light source.
Model BHA-P
C.
Rotate the trimmer screw until the voltmeter indicates
1V
(page
9)
D.
Place a specirnen slide on the mechanical stage.
E.
Remove the Bertrand lens and analyzer from the ligh! path.
F. Coarse focc~swith the
10X
objective.
G.
Make interpirpillary and diopter adjustments (page
10)
H.
Center the condenser (page
10)
I. Center the stage (page 12).
J.
Center objectives other than
10X
(page 12)
K.
Swing in the desired objective.
L.
Set the condenser, analyzer and Bertrand lens correctly according to your microscopic
purpose (pages 13 and 14).
M.
Adjust illumination system.
N. Adjust light intensity.
0.
Finefocus.
P.
Adjust aperture irisdiaphragm and field iris diaphragm (page
12).
(
Adjustment of illumination system
I
For biological use ol the Moclel BHA-P, however, remove the analyzer, Bertrand lens
and
sensitive tint plates, and place the high/low magnification selector lever into the
"L"
posi-
tion lor
4X
and
lox,
aricl the
"H"
positior?for
20X. 40X
and
100X
object~ves.
Microscopic
Orthoscopic
observation
Conoscopic
observation
*
Cut
off this page at dotted line and
put
~t on the wall near the microscope for
use
as
a
reminder of
microscopic procedure.
Objective
4X
to
100X
20X
to
100X
Intermediate
polarizingat-
tachment
OUT
IN
r
Conder~ser
top lens
OUT
IN
Highllow magnifica-
tion selector lever
L
H

V.
OPERATION
1.
Electric System
1)
Adjustment of Light Intensity
The rninimum voltage required for the light source can be adjusted with the rheostat
trimmer screw at the bottorri of the microscope base in accordance with the line voltage
and frequency.
A
silicon controlled rectifier (SCR)
is
provided lor output voltage
control. The
SCR
has the following advantages over conventional rheostat cor-itrols.
@
Extremely fine adjustment of light intensity can be easily achieved
@
Flickering of the bulb filament is elirninated and light intensity is stabilized.
@
Increased lifeexpectancy of the bulb.
2)
Adjustment of Minimum Line Voltage
@
Ascertain that the voltage selector switch is set to conform with the local mains
voltage. (This switch can be turned with a coin, and can be set to the following
voltages: 100V-110V-I20V or 220V-240V.)
(3
Ascertain that the sliding control lever is positioned closest to you (lowvoltage),and
then activate the mainswitch. The pilot lan-iplights up.
@
If the bulb is dimnily lit, and the voltmeter indicates about IV,the secondary voltage
is correct, and you have only to push the sliding control lever forward in order to
obtain optimum light intensity.
@
If the bulb does not light or lights up brightly immediately after switching on, rotate
the rheostat trimmer screw gradually w~tha coin, until the voltmeter indicates
about 1V.
3)
Light Source
The standard light source incorporates a 30W pre-centered tungsten filament bulb,
provided with a socket for positive contact, eliminating the problems of defective
contact and over-heating.
When used at the rated voltage 6V. the aver-age lrfe
of
the tungsten bulb LS30 is longer
than 200 hours. This is,.however, greatly reduced, if the bulb is used at higher voltage; for
instance, the bulb life is reduced to 1/50 at 8V. Therefore, it is advisable to avoid
prolonged use at readingsover 6V (inthe red rorie).
Ifthe light source should be used at high voltage constantly, it is reconirnended to usea
high intensity halogen bulb.
*
Do not switch the tungsten bulb on &ith the sliding control lever at high intensity posi-
tion (away from the user). It reduces bulb life.

2.
lnterpupillary Distance and Diopter Adjustments
1)
Insert the eyepiece v~ithcross hairs of your choice into
tile right eyepiece tube, align~ngthe positioning slot
@
and position~ngpi11
@
.
(Fig.
1)
*
When the eyepiece positioning pin is inserted into the
lower slot on the tube, the cross lines in the eyepiece
coincide with the vibration direction of polarizer and
analyzer at
0
settings. When inserted into the other
slot, the cross lines are at
45O
to the direction of
vibration.
2)
Looking through the iight eyepiece (with cross hairs)
with your right eye, rotate the d~opteradjustment ring
@
until the cross hairs are sharpty focused. (Fig.
2)
3)
Looking through the both eyepieces with both eyes,
adjust the interpupillary distance, sliding the knurled
dovetail slides
@
of the right and left eyepiece tubes,
cintil perfect binocular vis~onis obtained. Fig.
2
4)
Memorize your interpupillary distance setting by means of the scale
@
,
))!{
5)
Rotate the tube length adjustment ring
@
on the right eyepiece tube
to
match your
interpupillary distance setting which you obtained from the scale
6)
Look at the image through the right eyepiece with your right eyepiece v~ithyour right
eye and focc~son the specimen with the coarse and fine adjustment knobs.
7) Look at the image through the left eyepiece with yocrr left eye and rotate the tube length
adjustment ring
@)
to focus on the specimen without using the coarse and fine adjust-
ment knobs.
3.
Light Path Selection
The trinoc~.llartcrbe is provided with a light path selector knob to direct the light to the
observation tube or to the phototube.
Knob position
Pushed in all the way.
Pulled out all the way.
Amount of light
100%
into binocc~lartub:!
20%
into binocular tube
80%
into phototube
Application
Observation
Photomicrography

4.
Condenser Centration
1)
Br~ngthe
objective
10X
into the light path.
*
If
a
specimen
is
placed on the circular rotatable stage
without
a
mechanical stage it is recommended to hold
the peripheries of the specimen with the stage clips
provided to the circular stage.
Swing in the condenser top lens, and br~ngthe specimen
into focus.
Stop down the field iris diaphrag~nw~tliknurled ring
a.
A
slightly blurred image of the field diaphragm can now
be seen in the eyepiece. (Fig.
3)
Flg.
3
4) Move the condenser up and down to locus on the image of the field diaphragm.
*
If the specimen slide is too thick, it is sometimes impossible to obtain a sharply-focused
image.
5)
While widening the diameter of the field progressively,
iise
the condenser centering
screws
(3)
to bring the diaphragm iniage Into the center of view. (Fig.
3)
6)
Push the analyzer
@
into the light path, and make sure
that both polarizer and analyzer are set at position
"0"
to attain the "Crossed filter" position. Then loosen the
clamping screw
(2)
of the condenser.
(Fig,
4)
7)
Remove the speclmen o~~tof the light path so that a
transparent area comes into the light path. Keeping the
polarizer at the
"0"
position, rotate the polarirer rota-
.
.,
.
,
I++-&:
,--
j,i
tion ring
@
until the optimum extinction is obtained,
/--:,
then clamp the ring. (Fig.
4)
Fig.
4

5.
Centering the Stage
I)
Looking througlk llic eyeplece ancl objective
10X,
cleter~ninesolme particular point, as
you like,
In
the specimen irnage and coincidii thrs point with
thc
center of the cross
hairs of the eyepiece.
2)
Rotating the stage, coincide the center of
11111
the rotation oi the specimen's pointg~ith
I--
>--
-
--
the center of the cross hairs by means of
two centering screws
[I?
provided on tlie
stage. (Fig.
51
*
Repeat this procedure until the centra-
tion
is
secured.
6.
Centering the Objectives
This centration
is
necessary to all the
PO
objectives except
the pre-centered objective
POlOX.
1)
Connect a centering knob
9
to each centering screw
of
the circular rotatable stage. (Fig.
6)
21
By means
of
these two centering screws, coincide the
centers of the cross hairs and the rotation of the
specimen.
3)
After complete centration, remove the centering knobs. Fig.
6
7.
Use of Iris Diaphragms
1
)
Aperture iris diaphragm
Adjust the opening of the aperture iris diaphragm
according to the various conditions scrch as the numeri-
cal aperture of the objective, irnage contrast, depth of
focus, and flatness of field. Generally it is often prefera-
ble to stop do\nln the aperture iris diaphragm to the
70%
-
or
80%
of the N.A.of the objective.
After the eyepiece is removed from the observation
tube, if necessary, look through the observation tube
and check the opening of the aperture diaphragm at the
objective pupil.
2)
Field iris diaphragm
The field [!-isdiaphl-agm controls the diarnetel-
of
tlie ray bcrndle ~nlpingingon the speci-
lneri s~~rfaceand thus increases irriage definition.
Generally, it is prefel-abletoslightly increase the diameter ofthe field iris diaphragm until
it is just outside the field of view.
!

8.
Focusing Adjcistment
1)
Tension adjustment of coarse adjustment ltnobs
A
tension adjustment ring
IS
provided nc!xt to the
riqlit hand coarse adjustment
knob.
With this clevice the
terisior) of the coarse adjustment is freely adjustable
lo^.
eittier- heavy oi licltit movement rfependincl or1 opcrator
01-eference.
Iiowever, do not loosen thc: tension adjustrnent rlny too
rnoch, because the stage clrops, or the
fine
adjustrncnt
knobs sl111eas~ly. Fig.
7
*
Be careful not to rotate the right and left coarse adjust-
ment knobs
in
the opposite directions sim~iltaneously.
2)
Pre-focusing lever
This levci.
3)
is locked after coarse focirs has been
accomplished. It prevents further ~ipwardtravel of the
stage by nienns of the coarse adjustrnent knobs, and
autoniatically provides a liiniting stop if the stage is
lowered and then raised agaln. (Fig.
8)
FI~.
8
9.
Orthoscopic Observation
1)
Swing out tile top eris
of
the condensel
In
principle, polarizided light enters the light path, parallel to the optical axis, to enable
observalion of the optical characler~st~csof the specirnen. However, this method will
darken the field of view and lower the resolving power
of
the objective extremely. There-
fore, swing out the top lens of the condenser, using only the lower aperture of the lower
condenser lens.
2)
Insert the analy~erinto the light path, and attain the crossed filter position with analyzer
and polari7er at
0
setting.
At this pos~tori,the polari~ervibra~i
In
is in the north-south
direction, and the analyzer vibration in the east-west d~rection.To open the filter posi-
tion, pull out the analyrer rotation screw.
3)
Rotatc the stage until the extinction of the image is
attaned, and n)ove tlic
45'
click stop lever
$
toward
the operator.
(Fig,
9)
Frorn this position, it is easy to rotate the stage in
45'
Increments without having to ircfer to
the
angular
scale, and the stage clicks at the clla~onalposition, at
\vliicii position. the retardation angle
is
measured. To
relcase the
45O
cl~ckstops, push back the
45"
click slop
lever.
I
Fig.
9

4)
Insel-t the quarter \Nave pltite or sensitive tint plate into tlie slot in
:he
intcrmccliato
l)oIarr?in(j lul~e,
*
A
Berek compensator
is
optionally available to measure the birefringence of aspecimen.
Sensit~vetint plate Quarter
wave
plate
Berek
compensator
10.
Conoscopic Observation
1)
Swing in the top lens of the condenser, and illornrnate the specimen with no need to
immerse between the condenser and specimen slicle,
2)
Bring the specimen into foccrs, rotate the Bertrand lens tcrrrent ring into the
IN
position.
3)
Focus on the interference
(I~LI~C
formed at the back focal plane of the objective frorn
0.
)
I
20X
ro
100X.
The pinhole cap provided may be ~~sedin place of the eyepiece to directly view the
interference figure mentioned above. In this case, the Bertrdncl lens is disengaged.
11.
Photomicrography
1)
Photomicrographic equipment
Photomicrography i~iththe h4odel
BHA-P
requires photomicrographic equipment such as
the photomicrograpliic system camera, exposure meter, photo eyepiece, etc. Read the
lnsiruction manuals for each equipment, and follow the steps below:
a
It
is
recommended to use a low power photo eyepiece
FK2.5X.
@
Photor~iicrographicmagnification 1s same as witl-1 the standard optrcal tube length,
although the optical tube length for- this use
is
prolonged because of the
intermediate
polariilng tube.

VII.
OPTICAL
DATA
Immersion objective. Resolving power
is
obtained when the objective
is
used at the full
aperture diaphragm.
The eyepieces
K5X
and WFlOX incorporate a sliding eye shield. This eye shield can be
pulled out to prevent glare and loss of contrast caused byarnbient light hitting the eye lens.
0
W.D.
(Workingdistance):
The distance between the specimen orcover-glass and the nearest point of the objective.
PO20X
0.40
1.58
8.1
3
0.84
1OOX
15.56
1.05
200X
9.19
0.9
Objective Magnification
-
N.A.
W.D.
imml
Focal length
(mrn)
--
Resolving power
(
P
)
0
N.A. (Numerical aperture):
The n~~mericalaperture represents
a
perforrnance number whlch cocrld he compared to
the relative aperture (f-number) of a camera lens. N.A.values can be usecl for directly
comparing the resolving powers of all types of objectives. The larger
N.A..
the higher the
resolving power.
P04X
0.1 0
18.77
28.45
3.4
20X
300.0
5.25
40X
172.5
4.5
K5X
[
r::ber
21
)
WFlOX
1181
-
0
Resolving power:
The abllily of
a
lens to register small details. The resolving power of a lens
IS
measured by
its ability to separate two points.
P040X
0.65
0.6
1
4.33
0.52
(Spring
loaded)
200X
4.99
0.53
400X
3.03
0.45
POlOX
0.25
6.78
16.08
-
'
1.3
50X
48.0
2.1
1OOX
27.60
1.8
Total magnification
Focal depth
(
I*)
)ecW(mm)-
f
otal magnification
Focal depth
(
,U
Field of view (mm)
0
Focal depth:
The
disrance between the upper and lower limits of sharpness in the image formed by an
optical system.
A
POlOOX
1.30
0.1
1
1.81
.-
0.26
(Spring
loaded)
500X
1.05
--
0.21
1,OOOX
0.66
0.18
0
Field number:
t
A
number that repl-esentsthe diameter in
lnln
of the image of
the
field diapliragnl that is
formed bv the lens
~ri
front of it.
0
Field of view diameter: the actual sixof the field
of
view in mnl.
9

VIII. TROUBLESHOOTING
I
Troubles Causes
I
Remedies
I
1.
Optical System
I
'
(a)With the illuminator The highllow magnification selector Place the lever in correct position.
switched on, the field of lever is not correctly positioned.
I
view cannot be seen. The condenser is lowered exces- Raise the condenser to the upper
i-I
Analyzer and polarizer are in the
"crossed filter" position
("0:O").
Sei them at the position
"0:90"
or
"90:O".
(b)Thefield of view is cut off
or illuminated irregularly.
The nosepiece is notclick stopped. Slightly rotate the nosepiece until
1
clicks into position.
The nosepiece is not correctly at- Insert the sliding dovetail mount
tached to the stand. lnto the stand all the way, until it
stops, then lock.
The auxiliary lens is not correctly
attached.
The light path selector lever is stop-
ped midway.
The highllow magnificationselector
lever is not correctly positioned.
-
Correct the lens position.
Push the lever all the way.
Place the lever all the way.
The condenser is not correctly
mounted on the ring mount. Re-insert the condenser all the way.
The sensitive tint plate is stopped
midway. Push the plate all the way until
it
clicks.
In case of orthoscopic observation,
the condenser top lens stays in the
light path or stops midway.
Swing it out of the light path.
The field iris diaphragm
is
stopped
down excessive1y.
Dust oncondeqser top lens.
Open the diaphragm fully.
(c)Dust or dirt
is
visible in
the field of view.
Dirty specimens.
I
Dust on eyepiece.
I
The lamp is not correctly attached.
Dust or dirt on the glass surface at
the light exit onthe base.
Re-insert the lamp correctly.
Clean off the dust or dirt.

Remedies
Raise the condenser.
Operi tile diaphragm.
Mount the auxiliary lens.
Place the lever incorrect position.
Insert the sliding dovetail mount all
the way, until it stops, then lock.
Slightly rotate the nosepiece until
it clicks into
position.
Clean the objective.
Apply immersion oil.
Remove bubbles.
Use the designated oil.
Clean.
Adjust the illumination.
Insert the sliding dovetail mount
into the stand
all
the way, then
lock.
Slightly rotate the nosepiece until
it clicks into position.
Place the specirnen on the stage and
secure it with the specimen clips.
Insert the sliding dovetail mount all
the way, until it stops, then lock.
Slightly rotate the nosepiece until
it clicks inloposilion.
Cenler the condenser.
Mocrnt the lens correctly.
Place the lever in correct position.
Troubles
(cl
)Excessive irnage contrast.
(e) Pesolution problems:
0
1
fiage is not sharp.
0
lrscrfficient contrast.
lrnage
details
lack
defini-
tron.
D
(f)The field of view
is
partial-
lyout of focus.
(g)The image goes out
of
focus cccentr~cally.
Causes
The condenser
IS
lowered exces-
sively.
The aperture irls diaphragm is stop-
pet1 down excessive1
y.
The auxiliary lens is not mounted.
The high/low magnificationselector
lever is not correctly positioned.
The nosepiece
is
not correctly at-
tached.
The objective is not correctly posi-
tioned in the light path.
Dirt on objective front lens.
The immersion objective
is
used
without immersion oil.
Birbbles in the
immersion
oil.
The Olympcrs designted oil
IS
not
used.
Dirty specimen.
Dirt on condenser lens.
The specirnen is not properly il-
luminated.
The nosepiece is not correctly at-
tached.
The objective
IS
not correctly posi-
tioned in the light path.
The specimen is not correctly posi-
tioned on the stage.
The nosepiece is not correctly at-
tached.
The objective
is
not correctly posi-
tioned in the light path.
The condenser
is
out of center.
The auxiliary lens is not correctly
rnoc~ntecl.
The higti/low rnagnlfication selector
lever is stopped midway.

Troubles
-
--
-
!h)Wlien objectives are
changed, they are not par-
focal.
(i) Light intensity does no1
increase although the volt-
age is raised.
(1)
The condenser does not
come to the correct posi-
tion for optimum extinc-
tion.
(k)No conoscopic image can
be seen.
(I)The crossed filter position
Causes
-
-
-
-
-
.-
The mechanical tube length is not
correctly adjusted.
The condenser is not correctly cen-
tered.
The condenser
is
lowered exces-
sively.
The observation tube and condens-
er are not correctly mounted.
The condenser top lens is not in
the light path.
The analyzer is out of the light
Remedies
Adjiist with the tube length adjust-
ment rings on the observation tube.
Center the condenser.
Raisethe condenser.
Re-mo~lntthem correctly.
Swing it in.
Push it in.
is
not atte~ned.
I
path.
2.
Electric System
la)The illuminator
is
too
bright (ortoo dark).
(b)Output voltage for the il-
luminator cannot be reg-
ulated.
(c)The light flickers and the
intensity is unstable.
(d)Fuseburns out too often.
(e)The pilot I;~mpl~glitsb~~t
the bull2
docs
not.
The rheostat trimmer screw is not
matched to the mains voltage.
-
The mains voltage is too high (or
too low).
The rheostat trimmer screw is not
correctly adjusted.
The voltage selector switch is not
matched to the mains voltage.
The mains voltage is too lowor too
high.
The mains voltage is unstable.
The filament of the bulb is likely
to bi.rrn out.
Loose electrical con1~ection.
The fuse
IS
not
a
standard fuse.
The voltage selector switch is not
matched to the mains voltage.
The bulb
IS
burned out.
Loose electrical connection.
Adjust the trimmer screw to match.-B
the mains voltage.
Adjust the mains voltage with
a
variable voltage transformer.
Adjust the trimmer screw until the
voltmeter indicates
1
V.
Adjust the mains voltage selector
switch to the mains voltage.
Adjust the mains voltage with
a
variable voltage transformer.
Use avariable voltage transformer.
Replace the bulb.
Secure theconnection.
Use astandard fuse.
Match the swilch to the mains
voltage.
Replace the b1.115,
Secure the connection.
This manual suits for next models
1
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