Olympus BHA-P User manual

I
OLYMPUS POLARIZING MICROSCOPE
MODELS BHA -P&B;H.-P ATTACHMENT

i
This instruction rnancral has been written for the use of the Olyn~pusPolarizing Microscope
!,
Modcl CHA-P and Polarizirl!j Attach~nentModel 131-I-P. It is recommended to r-ead the
I
>
manual carefully in order to familiarize yourself fully with the use of Ihe microscope on the
!
polarizing attachment so lhal you can ob~an the best performance and ~ffectiveness.
t
IMPORTANT
Observe the following points carefully:
Operation
1. Always handle the microscope with the care it deserves, and avoid abrupt motions.
2. Avoid exposureof the microscope to direct sunlight. dust and vibration.
3. Only use the tension adjustment ringfor altering the tension of the coarse adjustment.
Do not twist the two coarse adjustment knobs in the opposite directions sirnultane-
ously, which
might cause damage.
4. Ascertain that the voltage selector switch on the base plate is set to conformwit11 the
local mainsvoltage.
5. Disconnect the line cord frorn the AC power outlet for fuse replacement.
Maintenance
1. Lenses must always be kept clean. Fine dust on lens surfaces should be blown or
wiped off by means
of an air blower or a clean brush. Carefi~llywipe off oil or finger-
prints deposited
on the lens surfaces with yaclzs moistened with a small amount of
xylene, alcohol or ether.
2. Do not use organic solutions lo wipe the surfaces of various components. Plastic
parts, especially,should be cleaned with a neutral detergent.
3. Never disassemble the rnicroscope for repair
4. The microscope should be stored in its container immediately aftel- use. li this is not
possible, it slioi~ld
be covered with ti vinyl dust cover. It is best to kcap objectives and
eyepieces in
a desiccator, containingcjcsiccants.
5. Disconnect the linecord frorn the AC power soirrce before fuse replacement.

CONTENTS
t. STANDARD EQUIPMENT . . . . . . . . . . . . . . . . . . .
it. NOMENCLATURE . . . . . . . . . . . . . . . . . . . .
I1 I. ASSEMBLY . . . . . . . . . . . . . . . . . . . . . . . .
1V. IDENTIFICATIONAND FUNCTION OF VARIOUS COMPONENTS . . .
V. OPERATION . . . . . . . . . . . . . . . . . . . . . . . .
1. ElectricSystem
2, lnterpupillary Distance and Diopter Adjustments . . . . . . . . . .
3. Light Path Selection
4. Centering the Condenser . . . . . . . . . . . .
5. Centering the Stage . . . , . . . . . . . . . . . . . . . .
6. Centering the Objectives
7. Use of Iris Diaphragms
8. Focusing Adjustment . . . . . . . . . . . . . . . . . . .
9. OrthoscopicObservation
10. Conoscopic Observation . . . . . . . . . . . , . . . . . .
11. Photomicrography
VI. OPTICAL
DATA . . . . . . . . . . . . . . . . . . . . . .
VII. TROUBLESHOOTING. . . . . .t . . . . . . . . . . . . .

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1. STANDARD EQUIPMENT
Optional Accessories: Mechanical stage for polarizing use Model AH-FMP
Berek cornpensalor Modsl AH-CTP
Qhjectivc (srrain-free) P020X
Model
Microscope stand wrth aux~lrary lens
BHA-F
Rcvo lving nosepiece RH-PRE
l~itermediate polarizing a~tachmenl AH-PA
Quarter wave plate AH-TPI47
BHA-751-P
J
1
1
BHA-651-P
1
1
1
1
pp
BH-P-1
I
0
1
I
1
Sensrt~ve tint plate (53OrnpCI) AH-TP530
Polarlztng b~necular tube (30~) BH-PB 130
Polari~lnq tr~nocular tube (30~1 BH-PTH30
Circular rotatable stage BH-SRP
Swing-out condenser BH -POC
Tungsten lamp house BH-LH
30-watt tungsten filament bulbs LS30
Objecrrves (strain-free)
BH-P-2
0
1
1
1
1
0
1
1
1
3
1
1
PO4 X
POIOX
P040X
PO1 00X Iollj
0
1
1
111
1
3
1
' 1
1
1
K5X (with cross hatrs) 1 1-1 1
7
WFlOX (with cross ha~rs} 1 1 1 1
Eyepieces WF1 OX (with 1011 00mm micrometer) 1
BIKSX
1 1 1 1
WFlOX 1 1 11
1
1
1
1
1
1
1
Photo ~yep~ece F K5X 0
0
Spare fuses
F~lter 45C
Immersion nil
I
V~nyt dust cover
-
1
1
0
0
0
0
-
2
1
1
1
1 10
-
2 0
I
1 I0
1
0
' ,!
0
1
\
1
1
0
1
1
1
1
t
1
1
0 0
0 0

II. NOMENCLATURE
Base 1

Ill. ASSEMBLY
Tfic 141ctl1re i~clow11111~t~atCsthe scq~leritidl ~prncodr~r 1% ol assernbly T!ie nt~nhrvc ~ndtcrlt~
the assembly cjrrlnr ol vilrlous colnponenls. Rel~iove dust caps before mounting Lurnpunents.
Take cars lo Ireep all glaqs surfaces cleat), and avalcl si.4 al~4t111g tlie srrr faces.
0 Eyepiece
Insert
the objective 10X inlo
the fixed aperture of the nose-
piece.
$1 Objective
"I
@ Qbservat~on tu11e Clamping screw
Quarter wave plate
Sensitive rint
@j lntermedrate Dolarizing tube
plate Attach the tube to the stand
--
wrth rhe letters "OLYMPUS"
Berek
facing in front of the micro-
compensator
scope (or align the red dots).
(TI Mechanical stage I 0 Nosepiece \ k---
Rotatable stage
M~croscops stand
@ Lamp house
Insert the two pins 01 the
aux~liary lens ~nto the bush.
rng5 on the microscoae stand
unt~l the lens clicks into posi-
tion.
w~th the convex surface
(with engraving "UPS1DE")
tac~ng upward.
Align~ng red dots, on condens-
er mount and condenser, Insert
the condenser inro the mount
Prior to mounting the stage.
rack down the condenser
mount all the way. To rernwe
the stage, (*move rhe cbn-
denser firs[.
Insert the lamp house and pres-
sing the clamping screw at the
bottom of the base, rotate he
lamp house until the locating
hole and clamplng screw are
al~sned.
, Electr~c connection
1) Pluq 111 11i(! t:o~hnr?ct~ng cord of th~ lari~p hocrsc 10 ~hc r~ccptactr: at tho back of the bds~
2) Inscr i onc plz~g ol the linc cord to the Illis cord socket on rhe llasc and coiincct the orher
or lug to rhe AC [lnwer outlet.

; )
IV. IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS
L~ght path selector kr~ol)
/
Pull out the selector knob all
the way for photomicrography.
Analyicl- scaic
Tension adjustment ring
Arrow mark ~ndicatesIncrease
in coarse adjustment tension.
Voltmeter
\ SIlcIinq cont~~ollever
For cont~nuouslyvariable light
intensity.
\ L~icvol~~g~ selector switch

Mer.lianical tube length adjust-
Clarnping screw for
mechanical stage
ngraved letters "IN" for in-
sertion
of Bertrand lens into
the light path;
"OUT" for
removal of the Bertrand lens
from the light
path.
45' click stop lever
Clamping screw for
stage
uated.
Filter mount
Polarizer scale
Reading to 5'.
Field iris diaphragm ring
Polarirer rotation ring
Rheostat trimmer screw
screwdriver until the voltmeter indicates IV,
with the slid~ng control lever positioned
closest to vou (low voltage).
Larnp house clamping screw

Nosepiece clamping
Automatic
the cap of the fuse holder, and
replace the defective fuse with
a replacement fuse.
(Disconnect BEFORE RE-
PLACEMENT.)

A. Match the linevoltage selector switch to local mains voltage (seepage 6).
Summary of Putting the Microscope in Operation
B. Switch on the light source.
Model BHA-P
C.
Rotate the trimmer screw until the voltmeter indicates 1V (page 9)
D.
Place a specirnen slide on the mechanical stage.
E.
Remove the Bertrand lens and analyzer from the ligh! path.
F. Coarse focc~swith the
10X objective.
G.
Make interpirpillary and diopter adjustments (page 10)
H.
Center the condenser (page 10)
I. Center the stage (page 12).
J.
Center objectives other than 10X (page 12)
K.
Swing in the desired objective.
L. Set the condenser, analyzer and Bertrand lens correctly according to your microscopic
purpose (pages 13 and 14).
M. Adjust illumination system.
N. Adjust light intensity.
0. Finefocus.
P.
Adjust aperture irisdiaphragm and field iris diaphragm (page 12).
( Adjustment of illumination system I
For biological use ol the Moclel BHA-P, however, remove the analyzer, Bertrand lens and
sensitive tint plates, and place the high/low magnification selector lever into the "L" posi-
tion lor
4X and lox, aricl the "H" positior?for 20X. 40X and 100X object~ves.
Microscopic
Orthoscopic
observation
Conoscopic
observation
*
Cut off this page at dotted line and put ~t on the wall near the microscope for use as a reminder of
microscopic procedure.
Objective
4X to 100X
20X
to 100X
Intermediate
polarizingat-
tachment
OUT
IN r
Conder~ser
top lens
OUT
IN
Highllow magnifica-
tion selector lever
L
H