Sakura Tissue-Tek TEC 6 User manual

Tissue-Tek®TEC™6
Cryo Module
Operating Manual
Part Number M01-022E-01
Revision Letter Original
Released 2018-08-03

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The brand names of products that have been registered or trademarked by and are owned
by Sakura Finetek USA, Inc., Sakura Finetek Japan Co., Ltd., and Sakura Finetek Europe B.V.
appear here:
Sakura
Tissue-Tek
Please visit our website www.sakura-netek.com
Manufactured for:
Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Europe B.V., 2408 AV Alphen aan den Rijn, NL
Sakura Finetek Japan Co., Ltd., Tokyo, 103-0023, Japan
Manufactured by:
Sakura Seiki Co., Ltd., Nagano, 387-0015, Japan
© 2018 Sakura Finetek Japan Co., Ltd.
All Rights Reserved
Printed in Japan.

Table of contents
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1. Introduction
1.1 Intended Use…………………………………………………………7
1.2 Safety Precautions …………………………………………………8
1.3 Precautions for Use……………………………………………… 13
1.4 Overview of the Instrument …………………………………… 14
1.4.1 Instrument Conguration ……………………………… 14
1.4.2 Specications …………………………………………… 15
1.5 Accessories ……………………………………………………… 16
1.5.1 Standard Accessories (Cryo Module) ………………… 16
1.6 Description of Instrument Parts ……………………………… 17
1.6.1 Instrument (Front Side) ………………………………… 17
1.6.2 Instrument (Rear Side) ………………………………… 17
2. Installation
2.1 Installation ………………………………………………………… 19
2.1.1 General Information on Installing the Instrument…… 19
2.1.2 Installation Environment………………………………… 19
2.1.3 Unpacking Method of the Cryo Module ……………… 20
2.1.4 Installation Method ……………………………………… 22
2.2 Setting Up the Instrument ……………………………………… 25
2.2.1 Temperature Setting for Each Part of the Instrument 25
2.2.2 Setting the Schedule of the Auto Operation (with the
Auto Cryo Mode) ………………………………………… 28
2.3 Operational Overview …………………………………………… 30
2.3.1 Operation Method ……………………………………… 30
3. Explanation of Operations
3.1 Explanation of Operations ……………………………………… 33
3.1.1 Basic Operations ………………………………………… 33
4. Error Log
4.1 Error Log ………………………………………………………… 35
4.1.1 Viewing the Error Log …………………………………… 35
5. Troubleshooting
5.1 Troubleshooting ………………………………………………… 37
5.1.1 Instrument Condition and Solution …………………… 37
5.1.2 Message Window………………………………………… 38
5.1.3 What to Do Following a Power Outage ……………… 44

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5.2 Cleaning…………………………………………………………… 45
5.2.1 Inspection and Cleaning ………………………………… 45
5.2.2 Cleaning the Cryo Module ……………………………… 45
6. Replacement Parts
6.1 Replacement Parts ……………………………………………… 47
6.1.1 Cryo Module …………………………………………… 47
7. Glossary of Terms
7.1 Glossary of Terms ……………………………………………… 49

Table of contents
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1. Introduction

1. Introduction
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1.1 Intended Use
The Tissue-Tek®TEC™6 Cryo Module (hereinafter referred to “the instrument”)
is intended to embed processed human or animal tissue in parafn by cooling
blocks which contain tissues and paraffin on cassettes after dispensing
molten paraffin. This instrument is used to facilitate as part of steps to
produce tissue specimens for histological studies and tests conducted in the
elds of pathology, anatomy, clinical pathology, etc.
The Cryo Module is controlled through the Embedding Module, so it must be
connected with a control cable. This document is the Operating Manual for
the Cryo Module. For more detail on how to operate the instrument, refer to
the Operating Manual for the Tissue-Tek®TEC™6 Embedding Module.
In the case of disposal or maintenance of the instrument, comply with
national, regional and local laws and regulations for equipment containing
hydrouorocarbon to ensure proper management and collection of HFC.
Carefully read this Operating Manual and use the instrument correctly so
that its performance can be utilized fully. Any other use of this instrument is
considered improper operation.

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1.2 Safety Precautions
Designate the "Instrument Control Manager."
• Operation of the instrument requires expert knowledge of the target
application, method of use, and so on. Therefore, to use the instrument
correctly and safely, designate an "Instrument Control Manager."
• When the instrument is delivered, the Instrument Control Manager should
receive explanation on the handling of the instrument directly from the
staff trained in the instrument or distributor.
Before using the instrument, read the "Safety Precautions" to ensure the
correct use of the instrument.
The cautionary instructions provided herein are intended to ensure that the
instrument will be used safely in a manner preventing operator from injury and
property damage. These instructions provide important safety information
that must be needed at all times.
In this manual, instructions pertaining to different levels of potential hazards
are classified and indicated respectively as danger, warning, caution and
note.
The denition of each hazard is dened below.
DANGER pertains to a potential hazard where failure to observe the specied
instruction may result in death or serious injury of the user or another person.
WARNING pertains to a potential hazard where failure to observe the
specified instruction may result in a serious injury of the user or another
person.
CAUTION pertains to a potential hazard where failure to observe the specied
instruction may result in damage to the instrument or other property, or affect
the process results.
NOTE pertains to an item to pay attention to or other useful information.
DANGER
WARNING
CAUTION

1. Introduction
9
The symbols used on the labels attached to the instrument are explained.
The labels bearing one of the following symbols provide particularly important
information you must know in order to ensure safety of the operator, improve
work efficiency and protect the instrument from damage. Be sure to check
these labels and understand the specified instructions before commencing
your work
A label bearing this symbol specifies an action that must be taken. Always
follow the instruction.
A label bearing this symbol specifies a prohibited item. Always follow the
instruction.
A label bearing this symbol specifies a precaution. Wrong handling against
the instruction may potentially expose the operator to danger or damage the
instrument, so be sure to follow the instruction.
A label bearing this symbol is provided near a high-temperature area. Exercise
caution against burns caused by high temperatures.
If any biologically hazardous substance is handled, follow applicable
regulations or guidelines in your country or region to ensure safety.

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WARNINGS:
• Follow instructions in this operating manual.
If the instrument is not used properly, it may induce failure of the
instrument, injury to operators, and/or damages to facilities.
• Observe the instructions described in the Operating Manual.
Failure to do so will lead to instrument failure, a risk of injury to the
user and damage to facilities.
• Do not disassemble or modify the instrument.
Do not disassemble or modify the instrument except for the areas
specified in this manual.The instrument may malfunction or cause
accident, thus creating a dangerous situation.
• Do not wet the instrument.
Do not wet the instrument except for the areas specified in this
manual. If the instrument gets wet, electrical current leak may occur
and cause re or electric shock.
• Do not operate the switches with a wet hand.
Failure to observe the instruction may result in electric shock.
• Do not connect/remove the power plug with a wet hand.
Failure to observe the instruction may result in electric shock.
• When connecting/removing the power plug, do so by holding the
plug.
Connecting/removing the power plug by holding parts other than the
plug may cause re or electric shock.
• Do not damage the power cord.
Do not forcibly bend, pull, twist or bundle the power cord. If the
damaged power cord is used, fire or electric shock may occur. If
the power cord or power plug has scratches or other abnormalities,
contact a Sakura instrument distributor or representative.
• Check the power cord and electrical outlet for damage and
accumulated dust periodically.
Failure to do so may cause re depending on the condition.